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Alves, Tânia Maria de Almeida
Zani, Carlos Leomar
Texto completo SciELO Brasil
[PMID]:29236928
[Au] Autor:Cota BB; Tunes LG; Maia DNB; Ramos JP; Oliveira DM; Kohlhoff M; Alves TMA; Souza-Fagundes EM; Campos FF; Zani CL
[Ad] Endereço:Laboratório de Química de Produtos Naturais Bioativos, Instituto René Rachou, Fundação Oswaldo Cruz-Fiocruz, Belo Horizonte, MG, Brasil.
[Ti] Título:Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood).
[So] Source:Mem Inst Oswaldo Cruz;113(2):102-110, 2018 Feb.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES The aims of this study were to isolate and determine the chemical structures of the compounds responsible for the antileishmanial activity of the organic extract from N. pseudotrichia. METHODS Compounds were isolated by chromatographic fractionation using semi-preparative high-performance liquid chromatography, and their chemical structures were determined by analytical and spectral data and by comparison with published data. The antileishmanial activity of the isolated compounds was evaluated in intracellular amastigote forms of Leishmania (Viannia) braziliensis expressing firefly luciferase as reporter gene, and cytotoxicity was determined in Vero and THP-1 mammalian cell lines by MTT assay. FINDINGS Fractionation of the extract yielded seven compounds: 10-acetyl trichoderonic acid A (1), 6'-acetoxy-piliformic acid (2), 5',6'-dehydropiliformic acid (3), piliformic acid (4), hydroheptelidic acid (5), xylaric acid D (6), and cytochalasin D (7). Compounds 1, 2 and 3 are reported here for the first time. Compounds 1, 2, and 5 were more active, with IC50 values of 21.4, 28.3, and 24.8 µM, respectively, and showed low toxicity to Vero and THP-1 cells. MAIN CONCLUSIONS N. pseudotrichia produces secondary metabolites that are more toxic to intracellular amastigote forms of L. (V.) braziliensis than to mammalian cells.
[Mh] Termos MeSH primário: Caesalpinia/microbiologia
Leishmania braziliensis/efeitos dos fármacos
Nectria/química
Tripanossomicidas/farmacologia
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular
Cercopithecus aethiops
Cromatografia Líquida de Alta Pressão
Concentração Inibidora 50
Testes de Sensibilidade Parasitária
Testes de Toxicidade
Tripanossomicidas/isolamento & purificação
Tripanossomicidas/toxicidade
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trypanocidal Agents)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


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[PMID]:28441723
[Au] Autor:Sánchez-Carranza JN; Alvarez L; Marquina-Bahena S; Salas-Vidal E; Cuevas V; Jiménez EW; Veloz G RA; Carraz M; González-Maya L
[Ad] Endereço:Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Mexico. jes_chazaq@hotmail.com.
[Ti] Título:Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines.
[So] Source:Molecules;22(4), 2017 Apr 22.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:( ), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines. The compounds isolated and identified by chromatography and spectroscopic analysis were stigmasterol, ethyl gallate and gallic acid. Cytotoxic assays on cancer cells showed different ranges of activities. A differential effect on cell cycle progression was observed by flow cytometry. In particular, ethyl gallate and tannic acid induced G2/M phase cell cycle arrest and showed interesting effect on microtubule stabilization in Hep3B cells observed by immunofluorescence. The induction of apoptosis was characterized by morphological characteristic changes, and was supported by increases in the ratio of Bax/Bcl-2 expression and activation of caspase 3/7. This work constitutes the first phytochemical and cytotoxic study of and showed the action of its phenolic constituents on cell cycle, cell death and microtubules organization.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Caesalpinia/química
Extratos Vegetais/farmacologia
Moduladores de Tubulina/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Proteínas Reguladoras de Apoptose/metabolismo
Proliferação Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos
Ácido Gálico/análogos & derivados
Ácido Gálico/isolamento & purificação
Ácido Gálico/farmacologia
Células HeLa
Células Hep G2
Seres Humanos
Concentração Inibidora 50
Microtúbulos/metabolismo
Extratos Vegetais/isolamento & purificação
Estabilidade Proteica
Taninos/isolamento & purificação
Taninos/farmacologia
Moduladores de Tubulina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Apoptosis Regulatory Proteins); 0 (Plant Extracts); 0 (Tannins); 0 (Tubulin Modulators); 235I6UDD3L (ethyl gallate); 632XD903SP (Gallic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


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[PMID]:28064563
[Au] Autor:Câmara AC; Gadelha IC; Castro MB; Medeiros RM; Riet-Correa F; Soto-Blanco B
[Ad] Endereço:Veterinary Hospital, Universidade Federal Rural do Semi-Árido, Mossoró, Rio Grande do Norte, Brazil (Câmara, Gadelha).
[Ti] Título:Embryotoxic effects of Poincianella ( Caesalpinia) pyramidalis leaves on pregnant rats.
[So] Source:J Vet Diagn Invest;29(2):137-142, 2017 Mar.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the embryotoxic and fetotoxic effects of Poincianella pyramidalis (Tul.) L.P. Queiroz (syn. Caesalpinia pyramidalis Tul.) leaves on pregnant rats ( Rattus norvegicus). Pregnant rats were divided into 4 groups: G1-fed a ration containing 5% P. pyramidalis leaves beginning on day 1 of pregnancy; G2-fed a ration containing 5% P. pyramidalis leaves commencing on day 6 of pregnancy; G3-fed a ration containing 10% P. pyramidalis leaves starting on day 6 of pregnancy; G4-control group. On day 21 of pregnancy, parameters of reproductive performance were recorded. Fetal growth was measured, and fetuses were subjected to external examination and identification of skeletal anomalies. Rations containing P. pyramidalis resulted in dead or undeveloped fetuses and reduced the number, length, and weight of the fetuses. Rations also increased postimplantation losses and the frequency of skeletal anomalies. Furthermore, P. pyramidalis was also responsible for dose-dependent lesions in the placentas. In conclusion, the ingestion of P. pyramidalis promotes fetal and placental damage.
[Mh] Termos MeSH primário: Caesalpinia
Embrião de Mamíferos/anormalidades
Folhas de Planta/toxicidade
Teratogênios/toxicidade
[Mh] Termos MeSH secundário: Animais
Feminino
Doenças das Cabras/etiologia
Cabras
Masculino
Placenta/patologia
Gravidez
Ratos
Ratos Wistar
Ovinos
Doenças dos Ovinos/etiologia
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Teratogens)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716682564


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[PMID]:27845264
[Au] Autor:Singh J; Kumar A; Sharma A
[Ad] Endereço:University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
[Ti] Título:Antianxiety activity guided isolation and characterization of bergenin from Caesalpinia digyna Rottler roots.
[So] Source:J Ethnopharmacol;195:182-187, 2017 Jan 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Caesalpinia digyna Rottler (Caesalpiniaceae) roots have been used traditionally for soothing nerves, as nervine tonic and febrifuge. The aim of the present study was to isolate the antianxiety constituent(s) from C. digyna roots following bioactivity guided fractionation approach. MATERIAL AND METHODS: Bioactive fraction F4 was subjected to column chromatography to get three sub-fractions (F4.1-F4.3) and a compound CD2. CD1 and CD2 were characterized using standard spectral techniques. Three fractions (F4.1-F4.3) were evaluated for antianxiety activity in mice using EPM. An effective dose of the antianxiety principle was optimized, and its activity was, further evaluated using open field and mirror chamber tests. RESULTS: CD1 and CD2 characterized as bergenin, exhibited significant antianxiety activity at 80mg/kg, po which was statistically comparable to that of diazepam (2mg/kg, po). However, F4.1-F4.3 were observed to be devoid of antianxiety activity. Bergenin also exhibited significant antianxiety activity in open field and mirrored chamber tests. CONCLUSIONS: Bergenin, isolated from roots of C. digyna, exhibits significant antianxiety activity at 80mg/kg, po in three different models for evaluating antianxiety activity. This activity of bergenin is being reported for the first time.
[Mh] Termos MeSH primário: Ansiolíticos/farmacologia
Ansiedade/prevenção & controle
Comportamento Animal/efeitos dos fármacos
Benzopiranos/farmacologia
Caesalpinia/química
Atividade Motora/efeitos dos fármacos
Extratos Vegetais/farmacologia
Raízes de Plantas/química
[Mh] Termos MeSH secundário: Animais
Ansiolíticos/isolamento & purificação
Ansiedade/psicologia
Benzopiranos/isolamento & purificação
Fracionamento Químico
Cromatografia em Gel
Diazepam/farmacologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Feminino
Masculino
Camundongos
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Anxiety Agents); 0 (Benzopyrans); 0 (Plant Extracts); L84RBE4IDC (bergenin); Q3JTX2Q7TU (Diazepam)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


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[PMID]:27588605
[Au] Autor:Li ZY; Li QZ; Ma GX; Chen L; Zhang C; Chen BD; Yang JS; Li WP
[Ad] Endereço:a Zhongshan School of Medicine , Sun Yat-sen University , Guangzhou 510080 , China.
[Ti] Título:Cassane-type diterpenes from Caesalpinia minax induce apoptosis in pituitary adenoma: structure-activity relationship, ER stress and Wnt/ß-catenin pathways.
[So] Source:J Asian Nat Prod Res;19(5):423-435, 2017 May.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plant-derived natural products have been the highly significant sources of novel antitumor agents. The cassane-type diterpenes of genus Caesalpinia have been reported to bear antiproliferative activities toward different types of cancer cells. In this study, we evaluated the antineoplasmic activities of 16 natural origin cassane-type diterpenes isolated from the CHCl extract of the seeds of C. minax in pituitary adenomas cells and identified caesalpin G (CAG) showed the strongest cytotoxicity. Moreover, we further investigated the structure-activity relationship and molecular mechanism of these derivatives systematically. The results confirmed the unsaturated lactone-type ring, hydroxyl at C-7, and alkenyl at C-11 or C-14 functionality as critical for anticancer activity in this family of natural products. In addition, the mechanism experiments also demonstrated unfolded protein response and ER stress and Wnt/ß-catenin pathway were involved in the CAG-induced apoptosis.
[Mh] Termos MeSH primário: Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Caesalpinia/química
Diterpenos/isolamento & purificação
Diterpenos/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/química
Apoptose/efeitos dos fármacos
Diterpenos/química
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Seres Humanos
Estrutura Molecular
Neoplasias Hipofisárias/tratamento farmacológico
Sementes/química
Relação Estrutura-Atividade
Proteínas Wnt/efeitos dos fármacos
beta Catenina/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Diterpenes); 0 (Drugs, Chinese Herbal); 0 (Wnt Proteins); 0 (beta Catenin); 0 (caesalpin G)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2016.1217520


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[PMID]:27484929
[Au] Autor:Chellappan DR; Purushothaman AK; Brindha P
[Ad] Endereço:Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, Thanjavur, India.
[Ti] Título:Gastroprotective potential of hydro-alcoholic extract of Pattanga (Caesalpinia sappan Linn.).
[So] Source:J Ethnopharmacol;197:294-305, 2017 Feb 02.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNO-PHARMACOLOGICAL RELEVANCE: Pattanga is botanically equated as Caesalpinia sappan Linn. (Family: Caesalpiniaceae) and is used in Ayurveda system of medicine since ages. According to Ayurveda, useful part is Heartwood, which is bitter, astringent and acrid and is useful in vitiated conditions of vata and pitta, burning sensation, wounds, ulcers, leprosy, skin diseases, menorrhagia, leucorrhea, and diabetes. It is used as a major ingredient in Ayurvedic formulations and preparations like Patrangasava, Chandanadya Thalia, and Karpuradyarka. AIM OF THE STUDY: The present study is planned to evaluate the gastroprotective activity of the selected Ayurvedic drug using three different in vivo gastric ulcer models, so as to provide scientific evidence for the Ayurvedic claims. MATERIALS AND METHODS: For this study, Wistar albino rats fasted overnight were selected. The hydroalcoholic extract of Caesalpinia sappan heartwood at the dose level 250 and 500mg/kg body weight was selected and administered orally before necrotizing agents. Antioxidant and antiulcer parameters were evaluated and the stomach samples were subjected for histopathological studies. In addition, PGE2 estimation and protein expressions of COX-1, COX-2 and iNOS were analyzed by Western blot. The plant extract was subjected to LCMS/MS analysis. In addition, Cytoprotective effect in isolated gastric mucosal cells, TUNEL Assay, Acid neutralizing capacity assay, H /K ATPase inhibitory assay were performed. RESULTS: The ulcer protection was found to be 92%, 86% and 64% against ethanol, NSAID and pylorus ligation induced ulcer respectively. The hydro-alcoholic extract of C. sappan heartwood exhibited cytoprotective effect with 76.82% reduction against indomethacin-induced cytotoxicity at the concentration of 25µg/ml. C. sappan showed 63.91% inhibition in H /K ATPase inhibitory assay at the concentration 500µg/ml. CONCLUSIONS: Our results depict that Caesalpinia sappan heartwood possesses gastroprotective activity, possibly mediated through cytoprotection and antioxidant mechanisms. The data obtained in the present study provides scientific support for the traditional use of Caesalpinia sappan in the management of peptic ulcer.
[Mh] Termos MeSH primário: Caesalpinia/química
Mucosa Gástrica/efeitos dos fármacos
Extratos Vegetais/farmacologia
Substâncias Protetoras/farmacologia
Úlcera Gástrica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/farmacologia
Antiulcerosos/química
Antiulcerosos/farmacologia
Antioxidantes/farmacologia
Ciclo-Oxigenase 1/metabolismo
Ciclo-Oxigenase 2/metabolismo
Dinoprostona/metabolismo
Etanol/química
Feminino
Mucosa Gástrica/metabolismo
ATPase Trocadora de Hidrogênio-Potássio/metabolismo
Indometacina/farmacologia
Proteínas de Membrana/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Fitoterapia/métodos
Extratos Vegetais/química
Substâncias Protetoras/química
Ratos
Ratos Wistar
Úlcera Gástrica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Anti-Ulcer Agents); 0 (Antioxidants); 0 (Membrane Proteins); 0 (Plant Extracts); 0 (Protective Agents); 3K9958V90M (Ethanol); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, rat); EC 1.14.99.1 (Cyclooxygenase 1); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (Ptgs1 protein, rat); EC 3.6.3.10 (H(+)-K(+)-Exchanging ATPase); K7Q1JQR04M (Dinoprostone); XXE1CET956 (Indomethacin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160804
[St] Status:MEDLINE


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[PMID]:27340793
[Au] Autor:Erharuyi O; Adhikari A; Falodun A; Jabeen A; Imad R; Ammad M; Choudhary MI; Gören N
[Ad] Endereço:Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
[Ti] Título:Cytotoxic, Anti-inflammatory, and Leishmanicidal Activities of Diterpenes Isolated from the Roots of Caesalpinia pulcherrima.
[So] Source:Planta Med;83(1-02):104-110, 2017 Jan.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A phytochemical investigation on the chloroform extract of roots led to the isolation of ten known furanocassane diterpenoids, vouacapen-5 -ol ( ), 8,9,11,14-didehydrovouacapen-5 -ol ( ), 6 -cinnamoyl-7 -hydroxyvouacapen-5 -ol ( ), pulcherrin A ( ), pulcherrin B ( ), pulcherrin J ( ), pulcherrimin A ( ), pulcherrimin B ( ), pulcherrimin C ( ), and pulcherrimin E ( ). Chemical transformation of and gave compounds 6 -hydroxyisovouacapenol C ( ), 6 -cinnamoyl-7 -acetoxyvouacapen-5 -ol ( ), and pulcherrimin D ( ). Cytotoxicity of compounds - was evaluated against three cancer cell lines (MCF-7, HeLa, and PC-3). Anti-inflammatory potential of the compounds was evaluated via the oxidative burst assay using a luminol-amplified chemiluminescence technique. Leishmanicidal activity was tested against promastigotes of . Compounds , and were found active against all three cancer cell lines with IC s ranging from 7.02 ± 0.31 to 36.49 ± 1.39 µM. Compounds and exhibited a potent inhibitory effect on reactive oxygen species generated from human whole blood phagocytes (IC = 15.30 ± 1.10 µM and 8.00 ± 0.80 µM, respectively). Compounds , and showed significant activity against promastigotes of (IC = 65.30 ± 3.20, 58.70 ± 2.80, and 55.90 ± 2.40 µM, respectively).
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Caesalpinia/química
Diterpenos/farmacologia
Tripanossomicidas/farmacologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Linhagem Celular Tumoral
Diterpenos/química
Diterpenos/isolamento & purificação
Seres Humanos
Estrutura Molecular
Raízes de Plantas/química
Tripanossomicidas/química
Tripanossomicidas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Diterpenes); 0 (Trypanocidal Agents)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE
[do] DOI:10.1055/s-0042-110407


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[PMID]:28044105
[Au] Autor:Cruz-Silva I; Nunes VA; Gozzo AJ; Praxedes-Garcia P; Tanaka AS; Shimamoto K; Araujo MS
[Ad] Endereço:Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, Brazil.
[Ti] Título:Protease Inhibitors Extracted from Lam. Affect Kinin Release during Lung Inflammation.
[So] Source:Pulm Med;2016:9425807, 2016.
[Is] ISSN:2090-1844
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an lung inflammation model in rats, in the presence or absence of CeKI ( kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases.
[Mh] Termos MeSH primário: Caesalpinia
Neutrófilos
Pneumonia
[Mh] Termos MeSH secundário: Animais
Catepsina G/metabolismo
Modelos Animais de Doenças
Cininogênios/metabolismo
Modelos Biológicos
Neutrófilos/efeitos dos fármacos
Neutrófilos/enzimologia
Compostos Fitoquímicos/farmacologia
Calicreína Plasmática/metabolismo
Pneumonia/tratamento farmacológico
Pneumonia/enzimologia
Inibidores de Proteases/farmacologia
Ratos
Sementes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Kininogens); 0 (Phytochemicals); 0 (Protease Inhibitors); EC 3.4.21.20 (Cathepsin G); EC 3.4.21.34 (Plasma Kallikrein)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1155/2016/9425807


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[PMID]:27966950
[Au] Autor:Qiao Y; Xu Q; Hu Z; Li XN; Xiang M; Liu J; Huang J; Zhu H; Wang J; Luo Z; Xue Y; Zhang Y
[Ad] Endereço:Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation and Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, Hubei Province, People's Republic of China.
[Ti] Título:Diterpenoids of the Cassane Type from Caesalpinia decapetala.
[So] Source:J Nat Prod;79(12):3134-3142, 2016 Dec 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eighteen compounds, including eight new cassane-type furanoditerpenoids, 3ß-hydroxyphanginin H (1), 3ß-acetoxyphanginin H (2), 7ß-acetoxyphanginin H (3), 7ß-hydroxyphanginin H (4), 4-epi-3ß-hydroxycaesalpinilinn (5), 4-epi-3ß-acetoxycaesalpinilinn (6), 20-acetoxytaepeenin D (7), and tomocin E (8), along with 10 known compounds (9-18) were isolated from the roots of Caesalpinia decapetala. Compounds 1-13 were isolated from C. decapetala for the first time. The new compounds with their absolute configurations were determined by extensive spectroscopic analysis, single-crystal X-ray diffraction, and electronic circular dichroism calculations. Compounds 1, 4, 5, 7, and 11 exhibited inhibitory activities against the SW1990 human pancreatic cancer cell line with IC values ranging from 2.9 to 8.9 µM.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Caesalpinia/química
Diterpenos/isolamento & purificação
Medicamentos de Ervas Chinesas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Cristalografia por Raios X
Ciclosporina/farmacologia
Diterpenos/química
Diterpenos/farmacologia
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Seres Humanos
Masculino
Camundongos Endogâmicos BALB C
Conformação Molecular
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Raízes de Plantas/classificação
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes); 0 (Drugs, Chinese Herbal); 83HN0GTJ6D (Cyclosporine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00910


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[PMID]:27852125
[Au] Autor:Sandoval TA; Urueña CP; Llano M; Gómez-Cadena A; Hernández JF; Sequeda LG; Loaiza AE; Barreto A; Li S; Fiorentino S
[Ad] Endereço:* Department of Microbiology, Grupo de Investigación Fitoquímica Universidad Javeriana (GIFUJ), Bogotá, Colombia.
[Ti] Título:Standardized Extract from Caesalpinia spinosa is Cytotoxic Over Cancer Stem Cells and Enhance Anticancer Activity of Doxorubicin.
[So] Source:Am J Chin Med;44(8):1693-1717, 2016.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Cancer stem cells (CSC) are the primary cell type responsible for metastasis and relapse. ABC-transporters are integral membrane proteins involved in the translocation of substrates across membranes protecting CSC from chemotherapeutic agents. A plant extract derived from C. spinosa (P2Et) previously investigated for its antitumor activity has been shown to reduce lung and spleen metastasis in mice that have been transplanted with breast cancer cells, suggesting that P2Et has a significant activity against cancer stem cells (CSC). P2Et extract was thoroughly characterized by HPLC/MS. The cytotoxicity of P2Et extract was evaluated using a MTT assay in human and murine cell lines with different profiles of resistance, by Pgp overexpression or by enrichment in cancer stem cells. The synergistic effect of P2Et with doxorubicin was evaluated in vitro in several cell lines and in vivo in mice transplanted with TS/A cells, a highly resistant cell line and enriched in CD44[Formula: see text]CD24[Formula: see text]CSC. The chromatographic fingerprint of P2Et extract revealed 13 gallotannins. We also found that P2Et extract was cytotoxic to cells regardless of their resistant phenotype. Similarly, complementary activities were observed as drug efflux reversion and antioxidant activity. Short-treatment with P2Et extract, revealed a synergistic effect with doxorubicin in resistant cell lines. In vivo the P2Et increases mice survival in a TS/A breast cancer model associated with augmentation of calreticulin expression. Our results suggest that P2Et treatment could be used as adjuvant along with conventional chemotherapy to treat tumors with a MDR phenotype or with high frequency of CSC.
[Mh] Termos MeSH primário: Antibióticos Antineoplásicos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Caesalpinia/química
Doxorrubicina/farmacologia
Células-Tronco Neoplásicas/patologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Calreticulina/metabolismo
Linhagem Celular Tumoral
Modelos Animais de Doenças
Sinergismo Farmacológico
Feminino
Seres Humanos
Taninos Hidrolisáveis/análise
Taninos Hidrolisáveis/isolamento & purificação
Camundongos Endogâmicos BALB C
Fitoterapia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Calreticulin); 0 (Hydrolyzable Tannins); 0 (Plant Extracts); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE



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