| [PMID]: | 28063388 |
| [Au] Autor: | Chen L; Tse WH; Chen Y; McDonald MW; Melling J; Zhang J |
| [Ad] Endereço: | Department of Chemical & Biochemical Engineering, University of Western Ontario, London, Ontario, Canada N6A 5B9. |
| [Ti] Título: | Nanostructured biosensor for detecting glucose in tear by applying fluorescence resonance energy transfer quenching mechanism. |
| [So] Source: | Biosens Bioelectron;91:393-399, 2017 May 15. |
| [Is] ISSN: | 1873-4235 |
| [Cp] País de publicação: | England |
| [La] Idioma: | eng |
| [Ab] Resumo: | In this paper, a nanostructured biosensor is developed to detect glucose in tear by using fluorescence resonance energy transfer (FRET) quenching mechanism. The designed FRET pair, including the donor, CdSe/ZnS quantum dots (QDs), and the acceptor, dextran-binding malachite green (MG-dextran), was conjugated to concanavalin A (Con A), an enzyme with specific affinity to glucose. In the presence of glucose, the quenched emission of QDs through the FRET mechanism is restored by displacing the dextran from Con A. To have a dual-modulation sensor for convenient and accurate detection, the nanostructured FRET sensors were assembled onto a patterned ZnO nanorod array deposited on the synthetic silicone hydrogel. Consequently, the concentration of glucose detected by the patterned sensor can be converted to fluorescence spectra with high signal-to-noise ratio and calibrated image pixel value. The photoluminescence intensity of the patterned FRET sensor increases linearly with increasing concentration of glucose from 0.03mmol/L to 3mmol/L, which covers the range of tear glucose levels for both diabetics and healthy subjects. Meanwhile, the calibrated values of pixel intensities of the fluorescence images captured by a handhold fluorescence microscope increases with increasing glucose. Four male Sprague-Dawley rats with different blood glucose concentrations were utilized to demonstrate the quick response of the patterned FRET sensor to 2µL of tear samples. |
| [Mh] Termos MeSH primário: |
Técnicas Biossensoriais/métodos
Glicemia/análise
Transferência Ressonante de Energia de Fluorescência/métodos
Glucose/análise
Pontos Quânticos/química
Lágrimas/química
|
| [Mh] Termos MeSH secundário: |
Animais
Compostos de Cádmio/química
Canavalia/química
Corantes/química
Concanavalina A/química
Dextranos/química
Hidrogel de Polietilenoglicol-Dimetacrilato/química
Masculino
Modelos Moleculares
Nanotubos/química
Nanotubos/ultraestrutura
Pontos Quânticos/ultraestrutura
Ratos Sprague-Dawley
Corantes de Rosanilina/química
Compostos de Selênio/química
Razão Sinal-Ruído
Silício/química
Sulfetos/química
Compostos de Zinco/química
Óxido de Zinco/química
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Blood Glucose); 0 (Cadmium Compounds); 0 (Coloring Agents); 0 (Dextrans); 0 (Rosaniline Dyes); 0 (Selenium Compounds); 0 (Sulfides); 0 (Zinc Compounds); 11028-71-0 (Concanavalin A); 12058M7ORO (malachite green); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); A7F646JC5C (cadmium selenide); IY9XDZ35W2 (Glucose); KPS085631O (zinc sulfide); SOI2LOH54Z (Zinc Oxide); Z4152N8IUI (Silicon) |
| [Em] Mês de entrada: | 1703 |
| [Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 170108 |
| [St] Status: | MEDLINE |
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