[PMID]: | 26542834 |
[Au] Autor: | Schulze AE; De Beer D; Mazibuko SE; Muller CJ; Roux C; Willenburg EL; Nyunaï N; Louw J; Manley M; Joubert E |
[Ad] Endereço: | Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa. |
[Ti] Título: | Assessing similarity analysis of chromatographic fingerprints of Cyclopia subternata extracts as potential screening tool for in vitro glucose utilisation. |
[So] Source: | Anal Bioanal Chem;408(2):639-49, 2016 Jan. |
[Is] ISSN: | 1618-2650 |
[Cp] País de publicação: | Germany |
[La] Idioma: | eng |
[Ab] Resumo: | Similarity analysis of the phenolic fingerprints of a large number of aqueous extracts of Cyclopia subternata, obtained by high-performance liquid chromatography (HPLC), was evaluated as a potential tool to screen extracts for relative bioactivity. The assessment was based on the (dis)similarity of their fingerprints to that of a reference active extract of C. subternata, proven to enhance glucose uptake in vitro and in vivo. In vitro testing of extracts, selected as being most similar (n = 5; r ≥ 0.962) and most dissimilar (n = 5; r ≤ 0.688) to the reference active extract, showed that no clear pattern in terms of relative glucose uptake efficacy in C2C12 myocytes emerged, irrespective of the dose. Some of the most dissimilar extracts had higher glucose-lowering activity than the reference active extract. Principal component analysis revealed the major compounds responsible for the most variation within the chromatographic fingerprints, as mangiferin, isomangiferin, iriflophenone-3-C-ß-D-glucoside-4-O-ß-D-glucoside, iriflophenone-3-C-ß-D-glucoside, scolymoside, and phloretin-3',5'-di-C-ß-D-glucoside. Quantitative analysis of the selected extracts showed that the most dissimilar extracts contained the highest mangiferin and isomangiferin levels, whilst the most similar extracts had the highest scolymoside content. These compounds demonstrated similar glucose uptake efficacy in C2C12 myocytes. It can be concluded that (dis)similarity of chromatographic fingerprints of extracts of unknown activity to that of a proven bioactive extract does not necessarily translate to lower or higher bioactivity. |
[Mh] Termos MeSH primário: |
Cromatografia Líquida de Alta Pressão/métodos Cyclopia (Planta)/química Avaliação Pré-Clínica de Medicamentos/métodos Glucose/metabolismo Células Musculares/metabolismo Extratos Vegetais/análise Extratos Vegetais/farmacologia
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[Mh] Termos MeSH secundário: |
Animais Transporte Biológico/efeitos dos fármacos Camundongos Células Musculares/efeitos dos fármacos
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[Pt] Tipo de publicação: | EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Plant Extracts); IY9XDZ35W2 (Glucose) |
[Em] Mês de entrada: | 1610 |
[Cu] Atualização por classe: | 161230 |
[Lr] Data última revisão:
| 161230 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 151107 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1007/s00216-015-9147-7 |
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