Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.184 [Categoria DeCS]
Referências encontradas : 36 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 4 ir para página            

  1 / 36 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27706097
[Au] Autor:Magcwebeba T; Swart P; Swanevelder S; Joubert E; Gelderblom W
[Ad] Endereço:Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland (Stellenbosch) 7602, South Africa. tmagcwebeba@sun.ac.za.
[Ti] Título:Anti-Inflammatory Effects of Aspalathus linearis and Cyclopia spp. Extracts in a UVB/Keratinocyte (HaCaT) Model Utilising Interleukin-1α Accumulation as Biomarker.
[So] Source:Molecules;21(10), 2016 Oct 02.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Ultraviolet B (UVB) radiation is one of the major predisposing risk factors of skin cancer. The anticancer and photoprotective effects of unoxidized rooibos ( ) and honeybush ( ) herbal teas, containing high levels of dihydrochalones and xanthones, respectively, have been demonstrated in skin cancer models in vivo. In the current study, the anti-inflammatory effects of methanol and aqueous extracts of these herbal teas were investigated in a UVB/HaCaT keratinocyte model with intracellular interleukin-1α (icIL-1α) accumulation as a biomarker. Extracts of green tea ( ) served as benchmark. Both extracts of green tea and rooibos, as well as the aqueous extract of , enhanced UVB-induced inhibition of cell viability, proliferation and induction of apoptosis, facilitating the removal of icIL-1α. The underlying mechanisms may involve mitochondrial dysfunction exhibiting pro-oxidant responses via polyphenol-iron interactions. The methanol extracts of honeybush, however, protected against UVB-induced reduction of cell growth parameters, presumably via antioxidant mechanisms that prevented the removal of highly inflamed icIL-1α-containing keratinocytes via apoptosis. The dual antioxidant and/or pro-oxidant role of the polyphenolic herbal tea constituents should be considered in developing preventive strategies against UVB-induced skin carcinogenesis. The indirect removal of UVB damaged keratinocytes by herbal tea extracts via apoptosis may find application in the prevention of photo-induced inflammation.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Fabaceae/química
Interleucina-1alfa/metabolismo
Queratinócitos/efeitos dos fármacos
Queratinócitos/efeitos da radiação
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Aspalathus/química
Biomarcadores/metabolismo
Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Cyclopia (Planta)/química
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Modelos Biológicos
Extratos Vegetais/química
Chá/química
Chás de Ervas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Biomarkers); 0 (IL1A protein, human); 0 (Interleukin-1alpha); 0 (Plant Extracts); 0 (Tea); 0 (Teas, Herbal)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


  2 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27671741
[Au] Autor:Magcwebeba TU; Riedel S; Swanevelder S; Swart P; De Beer D; Joubert E; Andreas Gelderblom WC
[Ad] Endereço:Department of Biochemistry, Stellenbosch University, Stellenbosch, South Africa.
[Ti] Título:The potential role of polyphenols in the modulation of skin cell viability by Aspalathus linearis and Cyclopia spp. herbal tea extracts in vitro.
[So] Source:J Pharm Pharmacol;68(11):1440-1453, 2016 Nov.
[Is] ISSN:2042-7158
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The relationship between polyphenol constituents, antioxidant properties of aqueous and methanol extracts of green tea (Camellia sinensis), the herbal teas, rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.), against skin cell viability was investigated in vitro. METHODS: The effect of extracts, characterised in terms of polyphenol content and antioxidant properties, on cell viability of premalignant, normal and malignant skin cells was determined. KEY FINDINGS: Phenolic composition, particularly high levels of potent antioxidants, of rooibos and green tea methanol extracts was associated with a strong reduction in cell viability specifically targeting premalignant cells. In contrast, the aqueous extracts of Cyclopia spp. were more effective in reducing cell viability. This correlated with a relatively high flavanol/proanthocyanidin content and ABTS radical cation scavenging capacity. The major green tea flavanol (epigallocatechin gallate) and rooibos dihydrochalcone (aspalathin) exhibited differential effects against cell viability, while the major honeybush xanthone (mangiferin) and flavanone (hesperidin) lacked any effect presumably due to a cytoprotective effect. The underlying mechanisms against skin cell viability are likely to involve mitochondrial dysfunction resulting from polyphenol-iron interactions. CONCLUSIONS: The polyphenol constituents and antioxidant parameters of herbal tea extracts are useful tools to predict their activity against skin cell survival in vitro and potential chemopreventive effects in vivo.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/farmacologia
Aspalathus/química
Camellia sinensis/química
Cyclopia (Planta)/química
Extratos Vegetais/farmacologia
Polifenóis/farmacologia
Lesões Pré-Cancerosas/tratamento farmacológico
Neoplasias Cutâneas/tratamento farmacológico
Pele/efeitos dos fármacos
Chá
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Antioxidantes/isolamento & purificação
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Peroxidação de Lipídeos/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Polifenóis/isolamento & purificação
Lesões Pré-Cancerosas/metabolismo
Lesões Pré-Cancerosas/patologia
Pele/metabolismo
Pele/patologia
Neoplasias Cutâneas/metabolismo
Neoplasias Cutâneas/patologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Plant Extracts); 0 (Polyphenols); 0 (Tea)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160928
[St] Status:MEDLINE
[do] DOI:10.1111/jphp.12629


  3 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27392243
[Au] Autor:Roza O; Martins A; Hohmann J; Lai WC; Eloff J; Chang FR; Csupor D
[Ad] Endereço:Department of Pharmacognosy, University of Szeged, Szeged, Hungary.
[Ti] Título:Flavonoids from Cyclopia genistoides and Their Xanthine Oxidase Inhibitory Activity.
[So] Source:Planta Med;82(14):1274-8, 2016 Sep.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The present paper reports the chemical analysis of the methanolic extracts of fermented and non-fermented Cyclopia genistoides herbs and an investigation of the xanthine oxidase inhibitory activity of the isolated constituents. Chemical analysis of the leaves and stems of C. genistoides yielded the isolation and identification of two benzophenone glucosides, iriflophenone 2-O-ß-glucopyranoside (1) and iriflophenone 3-C-ß-glucopyranoside (2), two pterocarpans, (6aR,11aR)-(-)-2-methoxymaackiain (5) and (6aR,11aR)-(-)-maackiain (6), along with the flavanones liquiritigenin (9) and hesperetin (10), the flavone diosmetin (11), the isoflavones afrormosin (7) and formononetin (8), piceol (3), and 4-hydroxybenzaldehid (4). Among the eleven compounds, nine are reported for the first time from this species, and six from the genus Cyclopia. These compounds, together with previously isolated secondary metabolites of this species, were tested for xanthine oxidase inhibitory activity. The 5,7-dihydroxyflavones luteolin and diosmetin significantly inhibited the enzyme in vitro, while hesperetin (10) and 5,7,3',5'-tetrahydroxyflavone exerted weak activity.
[Mh] Termos MeSH primário: Cyclopia (Planta)/química
Inibidores Enzimáticos/isolamento & purificação
Flavonoides/isolamento & purificação
Extratos Vegetais/farmacologia
Xantina Oxidase/antagonistas & inibidores
[Mh] Termos MeSH secundário: Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Flavonoides/química
Flavonoides/farmacologia
Estrutura Molecular
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Flavonoids); 0 (Plant Extracts); EC 1.17.3.2 (Xanthine Oxidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1055/s-0042-110656


  4 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27142456
[Au] Autor:Visser K; Zierau O; Macejová D; Goerl F; Muders M; Baretton GB; Vollmer G; Louw A
[Ad] Endereço:Biochemistry Department, Stellenbosch University, Stellenbosch, Western Cape, South Africa.
[Ti] Título:The phytoestrogenic Cyclopia extract, SM6Met, increases median tumor free survival and reduces tumor mass and volume in chemically induced rat mammary gland carcinogenesis.
[So] Source:J Steroid Biochem Mol Biol;163:129-35, 2016 10.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:SM6Met, a phytoestrogenic extract of Cyclopia subternata indigenous to the Western Cape province of South Africa, displays estrogenic attributes with potential for breast cancer chemoprevention. In this study, we report that SM6Met, in the presence of estradiol, induces a significant cell cycle G0/G1 phase arrest similar to the selective estrogen receptor modulator, tamoxifen. Furthermore, as a proof of concept, in the N-Methyl-N-nitrosourea induced rat mammary gland carcinogenesis model, SM6Met increases tumor latency by 7days and median tumor free survival by 42 days, while decreasing palpable tumor frequency by 32%, tumor mass by 40%, and tumor volume by 53%. Therefore, the current study provides proof of concept that SM6Met has definite potential as a chemopreventative agent against the development and progression of breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Cyclopia (Planta)/química
Glândulas Mamárias Animais/efeitos dos fármacos
Neoplasias Mamárias Experimentais/tratamento farmacológico
Fitoestrógenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Antagonistas de Estrogênios/farmacologia
Feminino
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Pontos de Checagem da Fase G1 do Ciclo Celular/genética
Seres Humanos
Glândulas Mamárias Animais/metabolismo
Glândulas Mamárias Animais/patologia
Neoplasias Mamárias Experimentais/induzido quimicamente
Neoplasias Mamárias Experimentais/mortalidade
Neoplasias Mamárias Experimentais/patologia
Metilnitrosoureia
Fitoestrógenos/isolamento & purificação
Ratos
Receptores Estrogênicos/antagonistas & inibidores
Receptores Estrogênicos/genética
Receptores Estrogênicos/metabolismo
Análise de Sobrevida
Tamoxifeno/farmacologia
Carga Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Estrogen Antagonists); 0 (Phytoestrogens); 0 (Receptors, Estrogen); 094ZI81Y45 (Tamoxifen); 684-93-5 (Methylnitrosourea)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE


  5 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26850159
[Au] Autor:Postma A; Slabbert E; Postma F; Jacobs K
[Ad] Endereço:Department of Microbiology, University of Stellenbosch, Private Bag X1, Stellenbosch 7602, South Africa 16234111@sun.ac.za.
[Ti] Título:Soil bacterial communities associated with natural and commercial Cyclopia spp.
[So] Source:FEMS Microbiol Ecol;92(3), 2016 Mar.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The commercially important plants in the genus Cyclopia spp. are indigenous to the Cape Floristic Region of South Africa and are used to manufacture an herbal tea known as honeybush tea. Growing in the low nutrient fynbos soils, these plants are highly dependent on symbiotic interactions with soil microorganisms for nutrient acquisition. The aim of this study was to investigate the soil bacterial communities associated with two commercially important Cyclopia species, namely C. subternata and C. longifolia. Specific interest was the differences between rhizosphere and bulk soil collected from natural sites and commercially grown plants. Samples were collected on two occasions to include a dry summer and wet winter season. Results showed that the dominant bacterial taxa associated with these plants included Acidobacteria, Actinobacteria, Bacteroidetes and Proteobacteria. Commercial and natural as well as rhizosphere and bulk soil samples were highly similar in bacterial diversity and species richness. Significant differences were detected in bacterial community structures and co-occurrence patterns between the wet and dry seasons. The results of this study improved our knowledge on what effect commercial Cyclopia plantations and seasonal changes can have on soil bacterial communities within the endemic fynbos biome.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Cyclopia (Planta)/microbiologia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/genética
Fenômenos Fisiológicos Bacterianos
Filogenia
Rizosfera
África do Sul
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160207
[St] Status:MEDLINE


  6 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26542834
[Au] Autor:Schulze AE; De Beer D; Mazibuko SE; Muller CJ; Roux C; Willenburg EL; Nyunaï N; Louw J; Manley M; Joubert E
[Ad] Endereço:Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.
[Ti] Título:Assessing similarity analysis of chromatographic fingerprints of Cyclopia subternata extracts as potential screening tool for in vitro glucose utilisation.
[So] Source:Anal Bioanal Chem;408(2):639-49, 2016 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Similarity analysis of the phenolic fingerprints of a large number of aqueous extracts of Cyclopia subternata, obtained by high-performance liquid chromatography (HPLC), was evaluated as a potential tool to screen extracts for relative bioactivity. The assessment was based on the (dis)similarity of their fingerprints to that of a reference active extract of C. subternata, proven to enhance glucose uptake in vitro and in vivo. In vitro testing of extracts, selected as being most similar (n = 5; r ≥ 0.962) and most dissimilar (n = 5; r ≤ 0.688) to the reference active extract, showed that no clear pattern in terms of relative glucose uptake efficacy in C2C12 myocytes emerged, irrespective of the dose. Some of the most dissimilar extracts had higher glucose-lowering activity than the reference active extract. Principal component analysis revealed the major compounds responsible for the most variation within the chromatographic fingerprints, as mangiferin, isomangiferin, iriflophenone-3-C-ß-D-glucoside-4-O-ß-D-glucoside, iriflophenone-3-C-ß-D-glucoside, scolymoside, and phloretin-3',5'-di-C-ß-D-glucoside. Quantitative analysis of the selected extracts showed that the most dissimilar extracts contained the highest mangiferin and isomangiferin levels, whilst the most similar extracts had the highest scolymoside content. These compounds demonstrated similar glucose uptake efficacy in C2C12 myocytes. It can be concluded that (dis)similarity of chromatographic fingerprints of extracts of unknown activity to that of a proven bioactive extract does not necessarily translate to lower or higher bioactivity.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cyclopia (Planta)/química
Avaliação Pré-Clínica de Medicamentos/métodos
Glucose/metabolismo
Células Musculares/metabolismo
Extratos Vegetais/análise
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Transporte Biológico/efeitos dos fármacos
Camundongos
Células Musculares/efeitos dos fármacos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151107
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-9147-7


  7 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26243020
[Au] Autor:Lee W; Yoon EK; Kim KM; Park DH; Bae JS
[Ad] Endereço:a College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 702-701, Republic of Korea.
[Ti] Título:Antiseptic effect of vicenin-2 and scolymoside from Cyclopia subternata (honeybush) in response to HMGB1 as a late sepsis mediator in vitro and in vivo.
[So] Source:Can J Physiol Pharmacol;93(8):709-20, 2015 Aug.
[Is] ISSN:1205-7541
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Cyclopia subternata is a medicinal plant commonly used in traditional medicine to relieve pain. In this study, we investigated the antiseptic effects and underlying mechanisms of vicenin-2 and scolymoside, which are 2 active compounds from C. subternata that act against high mobility group box 1 (HMGB1)-mediated septic responses in human umbilical vein endothelial cells (HUVECs) and mice. The antiseptic activities of vicenin-2 and scolymoside were determined by measuring permeability, neutrophil adhesion and migration, and activation of proinflammatory proteins in HMGB1-activated HUVECs and mice. According to the results, vicenin-2 and scolymoside effectively inhibited lipopolysaccharide-induced release of HMGB1, and suppressed HMGB1-mediated septic responses such as hyperpermeability, the adhesion and migration of leukocytes, and the expression of cell adhesion molecules. In addition, vicenin-2 and scolymoside suppressed the production of tumor necrosis factor-α and interleukin 6, and activation of nuclear factor-κB and extracellular regulated kinases 1/2 by HMGB1. Collectively, these results indicate that vicenin-2 and scolymoside could be a potential therapeutic agents for the treatment of various severe vascular inflammatory diseases via inhibition of the HMGB1 signaling pathway.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/farmacologia
Anti-Inflamatórios/farmacologia
Apigenina/farmacologia
Cyclopia (Planta)
Glucosídeos/farmacologia
Proteína HMGB1/farmacologia
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Luteolina/farmacologia
Sepse/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Apigenina/isolamento & purificação
Adesão Celular/efeitos dos fármacos
Permeabilidade da Membrana Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Células Cultivadas
Técnicas de Cocultura
Cyclopia (Planta)/química
Glucosídeos/isolamento & purificação
Proteína HMGB1/antagonistas & inibidores
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Mediadores da Inflamação/metabolismo
Lipopolissacarídeos/farmacologia
Luteolina/isolamento & purificação
Masculino
Camundongos Endogâmicos C57BL
Neutrófilos/efeitos dos fármacos
Neutrófilos/metabolismo
Fitoterapia
Plantas Medicinais
Sepse/metabolismo
Sepse/microbiologia
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Anti-Inflammatory Agents); 0 (Glucosides); 0 (HMGB1 Protein); 0 (HMGB1 protein, human); 0 (Inflammation Mediators); 0 (Lipopolysaccharides); 0 (apigenin-6,8-di-C-glycopyranoside); 0 (lipopolysaccharide, Escherichia coli O111 B4); 0 (scolymoside); 7V515PI7F6 (Apigenin); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160627
[Lr] Data última revisão:
160627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150806
[St] Status:MEDLINE
[do] DOI:10.1139/cjpp-2015-0021


  8 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26084452
[Au] Autor:Lee W; Ku SK; Bae JS
[Ad] Endereço:College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu, 702-701, Republic of Korea.
[Ti] Título:Ameliorative Effect of Vicenin-2 and Scolymoside on TGFBIp-Induced Septic Responses.
[So] Source:Inflammation;38(6):2166-77, 2015 Dec.
[Is] ISSN:1573-2576
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transforming growth factor ß-induced protein (TGFBIp) is an extracellular matrix protein whose expression in several cell types is greatly increased by TGF-ß. TGFBIp is released by the human umbilical vein endothelial cells (HUVECs) and functions as a mediator of experimental sepsis. Cyclopia subternata is a medicinal plant commonly used in traditional medicine to relieve pain in biological processes. In this study, we investigated the antiseptic effects and underlying mechanisms of vicenin-2 and scolymoside, two active compounds in C. subternata against TGFBIp-mediated septic responses in HUVECs and mice. The anti-inflammatory activities of vicenin-2 or scolymoside were determined by measuring permeability, human neutrophils adhesion and migration, and activation of pro-inflammatory proteins in TGFBIp-activated HUVECs and mice. According to the results, vicenin-2 or scolymoside effectively inhibited lipopolysaccharide-induced release of TGFBIp and suppressed TGFBIp-mediated septic responses, such as hyperpermeability, adhesion and migration of leukocytes, and expression of cell adhesion molecules. In addition, vicenin-2 or scolymoside suppressed the production of tumor necrosis factor-α and interleukin 6 and activation of nuclear factor-κB and extracellular regulated kinases 1/2 by TGFBIp. Vicenin-2 or scolymoside reduced cecal ligation and puncture (CLP)-induced septic mortality and pulmonary injury. Collectively, these results indicate that vicenin-2 and scolymoside could be a potential therapeutic agent for treatment of various severe vascular inflammatory diseases via inhibition of the TGFBIp signaling pathway.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Apigenina/farmacologia
Cyclopia (Planta)
Proteínas da Matriz Extracelular/metabolismo
Glucosídeos/farmacologia
Células Endoteliais da Veia Umbilical Humana/metabolismo
Luteolina/farmacologia
Extratos Vegetais/farmacologia
Sepse/prevenção & controle
Fator de Crescimento Transformador beta/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Apigenina/isolamento & purificação
Permeabilidade Capilar/efeitos dos fármacos
Adesão Celular/efeitos dos fármacos
Moléculas de Adesão Celular/metabolismo
Células Cultivadas
Quimiotaxia de Leucócito/efeitos dos fármacos
Cyclopia (Planta)/química
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Ativação Enzimática
Proteínas da Matriz Extracelular/imunologia
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Glucosídeos/isolamento & purificação
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/imunologia
Mediadores da Inflamação/metabolismo
Luteolina/isolamento & purificação
Masculino
Camundongos Endogâmicos C57BL
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
Neutrófilos/metabolismo
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Edema Pulmonar/imunologia
Edema Pulmonar/prevenção & controle
Sepse/imunologia
Sepse/metabolismo
Sepse/microbiologia
Fatores de Tempo
Fator de Crescimento Transformador beta/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cell Adhesion Molecules); 0 (Extracellular Matrix Proteins); 0 (Glucosides); 0 (Inflammation Mediators); 0 (Plant Extracts); 0 (Transforming Growth Factor beta); 0 (apigenin-6,8-di-C-glycopyranoside); 0 (scolymoside); 148710-76-3 (betaIG-H3 protein); 7V515PI7F6 (Apigenin); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150619
[St] Status:MEDLINE
[do] DOI:10.1007/s10753-015-0199-9


  9 / 36 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25969161
[Au] Autor:Beelders T; de Beer D; Joubert E
[Ad] Endereço:†Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa.
[Ti] Título:Thermal Degradation Kinetics Modeling of Benzophenones and Xanthones during High-Temperature Oxidation of Cyclopia genistoides (L.) Vent. Plant Material.
[So] Source:J Agric Food Chem;63(22):5518-27, 2015 Jun 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Degradation of the major benzophenones, iriflophenone-3-C-glucoside-4-O-glucoside and iriflophenone-3-C-glucoside, and the major xanthones, mangiferin and isomangiferin, of Cyclopia genistoides followed first-order reaction kinetics during high-temperature oxidation of the plant material at 80 and 90 °C. Iriflophenone-3-C-glucoside-4-O-glucoside was shown to be the most thermally stable compound. Isomangiferin was the second most stable compound at 80 °C, while its degradation rate constant was influenced the most by increased temperature. Mangiferin and iriflophenone-3-C-glucoside had comparable degradation rate constants at 80 °C. The thermal degradation kinetic model was subsequently evaluated by subjecting different batches of plant material to oxidative conditions (90 °C/16 h). The model accurately predicted the individual contents of three of the compounds in aqueous extracts prepared from oxidized plant material. The impact of benzophenone and xanthone degradation was reflected in the decreased total antioxidant capacity of the aqueous extracts, as determined using the oxygen radical absorbance capacity and DPPH(•) scavenging assays.
[Mh] Termos MeSH primário: Benzofenonas/química
Cyclopia (Planta)/química
Glucosídeos/química
Extratos Vegetais/química
Xantonas/química
[Mh] Termos MeSH secundário: Temperatura Alta
Cinética
Oxirredução
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzophenones); 0 (Glucosides); 0 (Plant Extracts); 0 (Xanthones)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150610
[Lr] Data última revisão:
150610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.5b01657


  10 / 36 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25419864
[Au] Autor:Beelders T; Brand DJ; de Beer D; Malherbe CJ; Mazibuko SE; Muller CJ; Joubert E
[Ad] Endereço:Department of Food Science, Stellenbosch University , Private Bag X1, Matieland 7602, South Africa.
[Ti] Título:Benzophenone C- and O-glucosides from Cyclopia genistoides (Honeybush) inhibit mammalian α-glucosidase.
[So] Source:J Nat Prod;77(12):2694-9, 2014 Dec 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An enriched fraction of an aqueous extract prepared from the aerial parts of Cyclopia genistoides Vent. yielded a new benzophenone di-C,O-glucoside, 3-C-ß-d-glucopyranosyl-4-O-ß-d-glucopyranosyliriflophenone (1), together with small quantities of a known benzophenone C-glucoside, 3-C-ß-d-glucopyranosylmaclurin (2). The isolated compounds showed α-glucosidase inhibitory activity against an enzyme mixture extracted from rat intestinal acetone powder. Compound 2 exhibited significantly (p < 0.05) higher inhibitory activity (54%) than 1 (43%) at 200 µM. In vitro tests in several cell models showed that 1 and its 3-C-monoglucosylated derivative (3-C-ß-d-glucopyranosyliriflophenone) were marginally effective (p ≥ 0.05) in increasing glucose uptake.
[Mh] Termos MeSH primário: Benzofenonas/isolamento & purificação
Benzofenonas/farmacologia
Cyclopia (Planta)/química
Glucosídeos/isolamento & purificação
Glucosídeos/farmacologia
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Inibidores de Glicosídeo Hidrolases/farmacologia
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Algoritmos
Animais
Benzofenonas/química
Glucose/metabolismo
Glucosídeos/química
Inibidores de Glicosídeo Hidrolases/química
Masculino
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Folhas de Planta/química
Ratos
África do Sul
alfa-Glucosidases/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3-C-beta-D-glucopyranosyl-4-O-beta-D-glucopyranosyliriflophenone); 0 (3-C-beta-D-glucopyranosylmaclurin); 0 (Benzophenones); 0 (Glucosides); 0 (Glycoside Hydrolase Inhibitors); EC 3.2.1.20 (alpha-Glucosidases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:141226
[Lr] Data última revisão:
141226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141125
[St] Status:MEDLINE
[do] DOI:10.1021/np5007247



página 1 de 4 ir para página            
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde