Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.187 [Categoria DeCS]
Referências encontradas : 49 [refinar]
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  1 / 49 MEDLINE  
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[PMID]:27678162
[Au] Autor:Chaumont L; Malécot V; Pymar R; Sbai C
[Ad] Endereço:LAREMA - UMR CNRS 6093, Université d'Angers, 2 bd Lavoisier, 49045 Angers Cedex 01, France.
[Ti] Título:Reconstructing pedigrees using probabilistic analysis of ISSR amplification.
[So] Source:J Theor Biol;412:8-16, 2017 Jan 07.
[Is] ISSN:1095-8541
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Data obtained from ISSR amplification may readily be extracted but only allows us to know, for each gene, if a specific allele is present or not. From this partial information we provide a probabilistic method to reconstruct the pedigree corresponding to some families of diploid cultivars. This method consists in determining for each individual what is the most likely couple of parent pair amongst all older individuals, according to some probability measure. The construction of this measure bears on the fact that the probability to observe the specific alleles in the child, given the status of the parents does not depend on the generation and is the same for each gene. This assumption is then justified from a convergence result of gene frequencies which is proved here. Our reconstruction method is applied to a family of 85 living accessions representing the common broom Cytisus scoparius.
[Mh] Termos MeSH primário: Alelos
Cytisus/genética
Modelos Genéticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:27824938
[Au] Autor:Shao F; Zhang Q; Liu H; Lu S; Qiu D
[Ad] Endereço:State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
[Ti] Título:Genome-Wide Identification and Analysis of MicroRNAs Involved in Witches'-Broom Phytoplasma Response in Ziziphus jujuba.
[So] Source:PLoS One;11(11):e0166099, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) play an important role in responding to biotic and abiotic stresses in plants. Jujube witches'-broom a phytoplasma disease of Ziziphus jujuba is prevalent in China and is a serious problem to the industry. However, the molecular mechanism of the disease is poorly understood. In this study, genome-wide identification and analysis of microRNAs in response to witches'-broom was performed. A total of 85 conserved miRNA unique sequences belonging to 32 miRNA families and 24 novel miRNA unique sequences, including their complementary miRNA* strands were identified from small RNA libraries derived from a uninfected and witches'-broom infected Z. jujuba plant. Differentially expressed miRNAs associated with Jujube witches'-broom disease were investigated between the two libraries, and 12 up-regulated miRNAs and 10 down- regulated miRNAs identified with more than 2 fold changes. Additionally, 40 target genes of 85 conserved miRNAs and 49 target genes of 24 novel miRNAs were predicted and their putative functions assigned. Using the modified 5'-RACE method, we confirmed that SPL and MYB were cleaved by miR156 and miR159, respectively. Our results provide insight into the molecular mechanisms of witches'-broom disease in Z. jujuba.
[Mh] Termos MeSH primário: Cytisus/genética
Cytisus/microbiologia
MicroRNAs/genética
Phytoplasma/patogenicidade
Doenças das Plantas/microbiologia
Ziziphus/genética
Ziziphus/microbiologia
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Regulação para Baixo/genética
Estudo de Associação Genômica Ampla/métodos
Filogenia
Doenças das Plantas/genética
Regulação para Cima/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (MicroRNAs)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166099


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[PMID]:27417986
[Au] Autor:Li B; Bewick S; Barnard MR; Fagan WF
[Ad] Endereço:Department of Mathematics, University of Louisville, Louisville, KY, 40059, USA. bing.li@louisville.edu.
[Ti] Título:Persistence and Spreading Speeds of Integro-Difference Equations with an Expanding or Contracting Habitat.
[So] Source:Bull Math Biol;78(7):1337-79, 2016 Jul.
[Is] ISSN:1522-9602
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We study an integro-difference equation model that describes the spatial dynamics of a species in an expanding or contracting habitat. We give conditions under which the species disperses to a region of poor quality where the species eventually becomes extinct. We show that when the species persists in the habitat, the rightward and leftward spreading speeds are determined by c, the speed at which the habitat quality increases or decreases in time, as well as [Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text], which are formulated in terms of the dispersal kernel and species growth rates in both directions. We demonstrate that in the case that the species grows everywhere in space, the rightward spreading speed is [Formula: see text] if c is relatively small and is [Formula: see text] if c is large, and the leftward spreading speed is one of [Formula: see text], [Formula: see text], or [Formula: see text]. We also show that it is possible for a solution to form a two-layer wave, with the propagation speeds of the two layers analytically determined.
[Mh] Termos MeSH primário: Ecossistema
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Afídeos
Mudança Climática
Simulação por Computador
Cytisus
Espécies Introduzidas/estatística & dados numéricos
Lagartos
Conceitos Matemáticos
Mariposas
Dinâmica Populacional/estatística & dados numéricos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1007/s11538-016-0180-2


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[PMID]:27105397
[Au] Autor:Rostgaard Nielsen L; Brandes U; Dahl Kjaer E; Fjellheim S
[Ad] Endereço:Department of Geosciences and Natural Resource Management, University of Copenhagen, Rolighedsvej 23, Frederiksberg C, 1958, Denmark.
[Ti] Título:Introduced Scotch broom (Cytisus scoparius) invades the genome of native populations in vulnerable heathland habitats.
[So] Source:Mol Ecol;25(12):2790-804, 2016 Jun.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytisus scoparius is a global invasive species that affects local flora and fauna at the intercontinental level. Its natural distribution spans across Europe, but seeds have also been moved among countries, mixing plants of native and non-native genetic origins. Hybridization between the introduced and native gene pool is likely to threaten both the native gene pool and the local flora. In this study, we address the potential threat of invasive C. scoparius to local gene pools in vulnerable heathlands. We used nuclear single nucleotide polymorphic (SNP) and simple sequence repeat (SSR) markers together with plastid SSR and indel markers to investigate the level and direction of gene flow between invasive and native heathland C. scoparius. Analyses of population structures confirmed the presence of two gene pools: one native and the other invasive. The nuclear genome of the native types was highly introgressed with the invasive genome, and we observed advanced-generation hybrids, suggesting that hybridization has been occurring for several generations. There is asymmetrical gene flow from the invasive to the native gene pool, which can be attributed to higher fecundity in the invasive individuals, measured by the number of flowers and seed pods. Strong spatial genetic structure in plastid markers and weaker structure in nuclear markers suggest that seeds spread over relatively short distances and that gene flow over longer distances is mainly facilitated by pollen dispersal. We further show that the growth habits of heathland plants become more vigorous with increased introgression from the invaders. Implications of the findings are discussed in relation to future management of invading C. scoparius.
[Mh] Termos MeSH primário: Cytisus/genética
Pool Gênico
Hibridização Genética
Espécies Introduzidas
[Mh] Termos MeSH secundário: Conservação dos Recursos Naturais
DNA de Plantas/genética
Dinamarca
Ecossistema
Fluxo Gênico
Genética Populacional
Genoma de Planta
Repetições de Microssatélites
Modelos Genéticos
Pólen/genética
Polimorfismo de Nucleotídeo Único
Sementes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.1111/mec.13666


  5 / 49 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:26871495
[Au] Autor:Cordero RL; Torchelsen FP; Overbeck GE; Anand M
[Ad] Endereço:School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada.
[Ti] Título:Cytisus scoparius (Fam. Fabaceae) in southern Brazil - first step of an invasion process?
[So] Source:An Acad Bras Cienc;88(1):149-54, 2016 Mar.
[Is] ISSN:1678-2690
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The occurrence of Scotch broom Cytisus scoparius (L.) Link (Fabaceae), is reported for the first time in Brazil. The species has been registered in the species-rich Campos Sulinos grasslands, in the Campos de Cima da Serra, and in the Serra do Sudeste. Naturalizing populations were frequently formed in natural habitats near to human settlements, where prevailing land uses and disturbances facilitate dispersal and establishment. The plant is an invasive species that has globally caused significant damage to biodiversity and economic losses. In Brazil, the species has a strong potential for spreading into a wide range of ecosystems. The Atlantic Forest biome and part of the Pampa biome, together known as the Campos Sulinos, represent optimal areas for the species. Features of the observed populations and recommendations for management are presented.
[Mh] Termos MeSH primário: Cytisus/crescimento & desenvolvimento
Espécies Introduzidas
[Mh] Termos MeSH secundário: Brasil
Cytisus/classificação
Florestas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE


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[PMID]:26628515
[Au] Autor:Hogg BN; Smith L; Daane KM
[Ti] Título:Impacts of the Adventive Psyllid Arytainilla spartiophila (Hemiptera: Psyllidae) on Growth of the Invasive Weed Cytisus scoparius Under Controlled and Field Conditions in California.
[So] Source:Environ Entomol;45(1):109-16, 2016 Feb.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The postrelease impact of weed biological control agents on their target weeds is rarely assessed. This study focuses on the impacts of the univoltine broom psyllid Arytainilla spartiophila Forster on the growth of its target weed, the invasive shrub Scotch broom, Cytisus scoparius (L.) Link (Fabaceae), in California. Arytainilla spartiophila is an adventive species that has been present in North America for several decades. In a greenhouse experiment, plant growth as measured by both height and total stem length (height + length of all branches) was reduced on plants that received psyllids, and psyllid densities were negatively correlated to proportional change in plant height. There were no effects on number of leaves, however. Furthermore, the psyllid had no statistically detectable impacts on plant growth parameters in a field experiment, underscoring how results obtained under controlled conditions may not fully predict the performance of biological control agents in the field. The high psyllid mortality that occurred at higher psyllid densities in both the greenhouse and the field suggests that the impacts of A. spartiophila may be moderated by intraspecific competition.
[Mh] Termos MeSH primário: Cytisus/crescimento & desenvolvimento
Hemípteros/fisiologia
Controle Biológico de Vetores
Plantas Daninhas/crescimento & desenvolvimento
Controle de Plantas Daninhas
[Mh] Termos MeSH secundário: Animais
California
Espécies Introduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151203
[St] Status:MEDLINE


  7 / 49 MEDLINE  
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[PMID]:26555402
[Au] Autor:Macci C; Peruzzi E; Doni S; Poggio G; Masciandaro G
[Ad] Endereço:a Consiglio Nazionale delle Ricerche (CNR) , Istituto per lo Studio degli Ecosistemi (ISE) , Pisa , Italy.
[Ti] Título:The phytoremediation of an organic and inorganic polluted soil: A real scale experience.
[So] Source:Int J Phytoremediation;18(4):378-86, 2016.
[Is] ISSN:1549-7879
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A phytoremediation process with horse manure, plants (Populus alba, Cytisus scoparius, Paulownia tomentosa) and naturally growing vegetation was carried out at a real-scale in order to phytoremediate and functionally recover a soil contaminated by metals (Zn, Pb, Cd, Ni, Cu, Cr), hydrocarbons (TPH) and polychlorobiphenyls (PCB). All the plants were effective in two years in the reclamation of the polluted soil, showing an average reduction of about 35%, 40%, and 70% in metals, TPH and PCB content, respectively. As regards the plants, the poplar contributed the most to organic removal. In fact, its ability to take up and detoxify organic pollutants is well known. Paulownia tomentosa, instead, showed high metal removal. The Cytisus scoparius was the least effective plant in soil decontamination. The recovery of soil functionality was followed by enzyme activities, expressing the biochemical processes underway, and nutrient content useful for plant growth and development. Throughout the area, an enhancement of metabolic processes and soil chemical quality was observed. All the enzymatic activities showed a general increase over time (until 3-4 fold than the initial value for urease and ß-glucosidase). Moreover, Cytisus scoparius, even though it showed a lower decontamination capability, was the most effective in soil metabolic stimulation.
[Mh] Termos MeSH primário: Cytisus/metabolismo
Recuperação e Remediação Ambiental/métodos
Lamiales/metabolismo
Populus/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cytisus/química
Recuperação e Remediação Ambiental/instrumentação
Hidrocarbonetos/análise
Hidrocarbonetos/metabolismo
Lamiales/química
Esterco/análise
Metais Pesados/análise
Metais Pesados/metabolismo
Populus/química
Poluentes do Solo/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons); 0 (Manure); 0 (Metals, Heavy); 0 (Soil Pollutants)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151112
[St] Status:MEDLINE
[do] DOI:10.1080/15226514.2015.1109595


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[PMID]:26243687
[Au] Autor:Niu S; Fan G; Deng M; Zhao Z; Xu E; Cao L
[Ad] Endereço:Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinsui District, Zhengzhou, 450002, Henan, People's Republic of China.
[Ti] Título:Discovery of microRNAs and transcript targets related to witches' broom disease in Paulownia fortunei by high-throughput sequencing and degradome approach.
[So] Source:Mol Genet Genomics;291(1):181-91, 2016 Feb.
[Is] ISSN:1617-4623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Paulownia witches' broom (PaWB) caused by the phytoplasma is a devastating disease of Paulownia trees. It has caused heavy yield losses to Paulownia production worldwide. However, knowledge of the transcriptional and post-transcriptional regulation of gene expression by microRNAs (miRNAs), especially miRNAs responsive to PaWB disease stress, is still rudimentary. In this study, to identify miRNAs and their transcript targets that are responsive to PaWB disease stress, six sequencing libraries were constructed from healthy (PF), PaWB-infected (PFI), and PaWB-infected, 20 mg L(-1) methyl methane sulfonate-treated (PFI20) P. fortunei seedlings. As a result, 95 conserved miRNAs belonging to 18 miRNA families, as well as 122 potential novel miRNAs, were identified. Most of them were found to be a response to PaWB disease-induced stress, and the expression levels of these miRNAs were validated by quantitative real-time PCR analysis. The study simultaneously identified 109 target genes from the P. fortunei for 14 conserved miRNA families and 24 novel miRNAs by degradome sequencing. Furthermore, the functions of the miRNA targets were annotated based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The results presented here provide the groundwork for further analysis of miRNAs and target genes responsive to the PaWB disease stress, and could be also useful for addressing new questions to better understand the mechanisms of plant infection by phytoplasma in the future.
[Mh] Termos MeSH primário: Infecções Bacterianas/genética
Cytisus/genética
Regulação da Expressão Gênica de Plantas/genética
Magnoliopsida/genética
MicroRNAs/genética
Phytoplasma/genética
Doenças das Plantas/genética
[Mh] Termos MeSH secundário: Infecções Bacterianas/microbiologia
Cytisus/microbiologia
Genes de Plantas/genética
Magnoliopsida/microbiologia
Doenças das Plantas/microbiologia
Plântulas/genética
Plântulas/microbiologia
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150806
[St] Status:MEDLINE
[do] DOI:10.1007/s00438-015-1102-y


  9 / 49 MEDLINE  
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PubMed Central Texto completo
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[PMID]:26545585
[Au] Autor:Schnippel K; Michelow P; Chibwesha CJ; Makura C; Lince-Deroche N; Goeieman B; Mulongo M; Jordaan S; Firnhaber C
[Ad] Endereço:Right to Care, Johannesburg, South Africa. kschnipp@gmail.com.
[Ti] Título:Cost-effectiveness of using the Cervex-Brush (broom) compared to the elongated spatula for collection of conventional cervical cytology samples within a high-burden HIV setting: a model-based analysis.
[So] Source:BMC Health Serv Res;15:499, 2015 Nov 06.
[Is] ISSN:1472-6963
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: From 2010 to 2014, approximately 2 million Pap smears from HIV-infected women were submitted to the South African National Health Laboratory Services (NHLS) through the national cervical cancer screening programme. The objective of this analysis was to determine whether using the plastic Cervex brush ("broom") would be a cost-effective approach to improve cytology specimen quality as compared to the wooden spatula used currently. METHODS: A decision analysis model was built using the expected adequacy rates for samples collected with the spatula (<$0.02) and broom ($0.23) and the probability of detecting cervical dysplasia. NHLS data was used for testing volumes and rates of HIV-positivity, suitability of specimens, and presence of endocervical cells. Expected positivity of Pap smears in HIV-infected women (73 %), odds ratios of the effectiveness of the broom (OR: 1.57), and improved sensitivity when endocervical cells present (OR: 1.89) are from literature. NHLS costs were used for the collection devices and conventional cytology ($4.89). Cost of clinic visit is from WHO CHOICE ($8.36). RESULTS: In 2010, 80 % of specimens submitted to NHLS were adequate for evaluation; in 2014, only 54 % met the same criteria. For HIV-infected women, according to the guidelines model, using the wooden spatula costs $6.25 million per year, $16.79 per woman tested. Under intended practice, for each additional HSIL case detected among HIV-infected women, the South African cervical cancer screening programme could save $13.64 (95 % CI: $13.52 to $13.76) by using the broom as its standard of care collection device through increased collection of endocervical cells and consequent reduction in repeat Pap smears. CONCLUSION: Under a wide range of parameters tested using a simulation model, the more expensive plastic broom could save the South African cervical cancer screening programme money and increase detection of high-grade cervical dysplasia in HIV-infected women compared to the current wooden spatula.
[Mh] Termos MeSH primário: Análise Custo-Benefício
Infecções por HIV
Teste de Papanicolaou/instrumentação
Manejo de Espécimes/instrumentação
Instrumentos Cirúrgicos/economia
Esfregaço Vaginal/instrumentação
[Mh] Termos MeSH secundário: Cytisus
Feminino
Seres Humanos
Laboratórios
África do Sul
Neoplasias do Colo do Útero/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151108
[St] Status:MEDLINE
[do] DOI:10.1186/s12913-015-1163-y


  10 / 49 MEDLINE  
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[PMID]:26416239
[Au] Autor:Pérez Galende P; Hidalgo Cuadrado N; Kostetsky EY; Roig MG; Villar E; Shnyrov VL; Kennedy JF
[Ad] Endereço:Centro de Investigación y Desarrollo Tecnológico del Agua (CIDTA), Universidad de Salamanca, 37007 Salamanca, Spain.
[Ti] Título:Kinetics of Spanish broom peroxidase obeys a Ping-Pong Bi-Bi mechanism with competitive inhibition by substrates.
[So] Source:Int J Biol Macromol;81:1005-11, 2015 Nov.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In plants, adverse conditions often induce an increase in reactive oxygen species (ROS) such as hydrogen peroxide (H2O2). H2O2 is reduced to water, and thus becomes detoxified by enzymes such as Cytisus multiflorus peroxidase (CMP). Here, the steady-state kinetics of the H2O2-supported oxidation of different organic substrates by CMP was investigated. Analysis of the initial rates vs. H2O2 and reducing substrate concentrations proved to be consistent with a substrate-inhibited Ping-Pong Bi-Bi reaction mechanism. The phenomenological approach expresses the peroxidase Ping-Pong mechanism in the form of the Michaelis-Menten equation and affords an interpretation of the effects in terms of the kinetic parameters [Formula: see text] , [Formula: see text] , kcat, [Formula: see text] , [Formula: see text] and of the microscopic rate constants, k1 and k3, of the shared three-step catalytic cycle of peroxidases.
[Mh] Termos MeSH primário: Cytisus/enzimologia
Peroxidase/metabolismo
[Mh] Termos MeSH secundário: Biocatálise
Guaiacol/metabolismo
Peróxido de Hidrogênio/metabolismo
Cinética
Modelos Moleculares
Oxirredução
Peroxidase/antagonistas & inibidores
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
6JKA7MAH9C (Guaiacol); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150930
[St] Status:MEDLINE



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