Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.192 [Categoria DeCS]
Referências encontradas : 27 [refinar]
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  1 / 27 MEDLINE  
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[PMID]:28196677
[Au] Autor:Pinto-Junior VR; Osterne VJ; Santiago MQ; Lossio CF; Nagano CS; Rocha CR; Nascimento JC; Nascimento FL; Silva IB; Oliveira AS; Correia JL; Leal RB; Assreuy AM; Cavada BS; Nascimento KS
[Ad] Endereço:Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
[Ti] Título:Molecular modeling, docking and dynamics simulations of the Dioclea lasiophylla Mart. Ex Benth seed lectin: An edematogenic and hypernociceptive protein.
[So] Source:Biochimie;135:126-136, 2017 Apr.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Lectins are proteins, or glycoproteins, capable of reversibly binding to specific mono- or oligosaccharides via a noncatalytic domain. The Diocleinae subtribe presents lectins with high structural similarity, but different effects based on biological activity assays. This variability results from small structural differences. Therefore, in this context, the present study aimed to perform a structural analysis of the lectin from Dioclea lasiophylla Mart. ex Benth seeds (DlyL) and evaluate its inflammatory effect. To accomplish this, DlyL was purified in a single step by affinity chromatography on Sephadex G-50 matrix. DlyL primary structure was determined through a combination of tandem mass spectrometry and DNA sequencing. DlyL showed high similarity with other species from the same genus. Its theoretical three-dimensional structure was predicted by homology modelling, and the protein was subjected to ligand screening with monosaccharides, oligosaccharides and complex N-glycans by molecular docking. Stability and binding of the lectin with α-methyl-d-mannoside were assessed by molecular dynamics. DlyL showed acute inflammatory response with hypernociceptive effect in the paw edema model, possibly by interaction with glycans present at the cell surface.
[Mh] Termos MeSH primário: Lectinas/química
Simulação de Acoplamento Molecular
[Mh] Termos MeSH secundário: Dioclea/química
Simulação de Dinâmica Molecular
Monossacarídeos/química
Oligossacarídeos/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lectins); 0 (Monosaccharides); 0 (Oligosaccharides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE


  2 / 27 MEDLINE  
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[PMID]:28130130
[Au] Autor:Pinto-Junior VR; Osterne VJ; Santiago MQ; Correia JL; Pereira-Junior FN; Leal RB; Pereira MG; Chicas LS; Nagano CS; Rocha BA; Silva-Filho JC; Ferreira WP; Rocha CR; Nascimento KS; Assreuy AM; Cavada BS
[Ad] Endereço:Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
[Ti] Título:Structural studies of a vasorelaxant lectin from Dioclea reflexa Hook seeds: Crystal structure, molecular docking and dynamics.
[So] Source:Int J Biol Macromol;98:12-23, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The three-dimensional structure of Dioclea reflexa seed lectin (DrfL) was studied in detail by a combination of X-ray crystallography, molecular docking and molecular dynamics. DrfL was purified by affinity chromatography using Sephadex G-50 matrix. Its primary structure was obtained by mass spectrometry, and crystals belonging to orthorhombic space group P2 2 2 were grown by the vapor diffusion method at 293K. The crystal structure was solved at 1.765Å and was very similar to that of other lectins from the same subtribe. The structure presented R and R of 21.69% and 24.89%, respectively, with no residues in nonallowed regions of Ramachandran plot. Similar to other Diocleinae lectins, DrfL was capable of relaxing aortic rings via NO induction, with CRD participation, albeit with low intensity (32%). In silico analysis results demonstrated that DrfL could strongly interact with complex N-glycans, components of blood vessel glycoconjugates. Despite the high similarity among Diocleinae lectins, it was also reported that each lectin has unique CRD properties that influence carbohydrate binding, resulting in different biological effects presented by these molecules.
[Mh] Termos MeSH primário: Dioclea/química
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Lectinas de Plantas/química
Lectinas de Plantas/farmacologia
Sementes/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Cristalografia por Raios X
Manosídeos/química
Manosídeos/metabolismo
Lectinas de Plantas/metabolismo
Polissacarídeos/química
Polissacarídeos/metabolismo
Domínios Proteicos
Ratos
Vasodilatadores/química
Vasodilatadores/metabolismo
Vasodilatadores/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannosides); 0 (Plant Lectins); 0 (Polysaccharides); 0 (Vasodilator Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


  3 / 27 MEDLINE  
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[PMID]:27664589
[Au] Autor:de Sousa BL; Nagano CS; Simões RD; Silva-Filho JC; Cunha RM; Cajazeiras JB; do Nascimento KS; Cavada BS
[Ad] Endereço:Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60455-970, Fortaleza, Ceará, Brazil; Departamento de Física, Universidade Federal do Ceará, 60455-760, Fortaleza, Ceará, Brazil. Electronic address: brunolopesdesousa@gmail.com.
[Ti] Título:Structure prediction and functional analysis of a non-permutated lectin from Dioclea grandiflora.
[So] Source:Biochimie;131:54-67, 2016 Dec.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Legume lectins have been widely studied and applied for many purposes in the last few decades, but many of their physiological aspects remain elusive. The Diocleinae legume subtribe, which includes intensively explored lectins, such as ConA, presents an unusual and extensive post-translational process which results in minor alterations in protein structure, in turn making its function elusive. Despite previous reports about Diocleinae precursor activity, no structural or functional analyses have ever been carried out to understand the impacts of post-translational processing relative to lectin structure and binding specificity. Here we analyzed the functionality of a non glycosylated, recombinantly expressed lectin precursor from Dioclea grandiflora through inhibition assays, corroborating the experimental data with structural information generated by molecular modeling, docking calculations and molecular dynamics simulations. We demonstrate that Diocleinae precursors are active and share the same carbohydrate specificity as mature lectins. At the same time, however, subtle structural alterations were detected and mostly result in an "incomplete" functionality of the precursor, as consequence of an immature binding site and an unstructured tetramer interface, affecting carbohydrate binding and oligomer formation, respectively.
[Mh] Termos MeSH primário: Dioclea/metabolismo
Lectinas de Plantas/química
Domínios Proteicos
Proteínas Recombinantes/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação/genética
Carboidratos/química
Biologia Computacional/métodos
Cristalografia por Raios X
Dioclea/genética
Eletroforese em Gel de Poliacrilamida
Escherichia coli/genética
Simulação de Dinâmica Molecular
Lectinas de Plantas/genética
Lectinas de Plantas/metabolismo
Ligação Proteica
Precursores de Proteínas/química
Precursores de Proteínas/genética
Precursores de Proteínas/metabolismo
Proteínas Recombinantes/metabolismo
Análise de Sequência de Proteína
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Dioclea grandiflora lectin); 0 (Plant Lectins); 0 (Protein Precursors); 0 (Recombinant Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


  4 / 27 MEDLINE  
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[PMID]:26499084
[Au] Autor:Barroso-Neto IL; Delatorre P; Teixeira CS; Correia JL; Cajazeiras JB; Pereira RI; Nascimento KS; Laranjeira EP; Pires AF; Assreuy AM; Rocha BA; Cavada BS
[Ad] Endereço:Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
[Ti] Título:Structural analysis of a Dioclea sclerocarpa lectin: Study on the vasorelaxant properties of Dioclea lectins.
[So] Source:Int J Biol Macromol;82:464-70, 2016 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lectins are proteins that show a variety of biological activities. However, they share in common at least one domain capable of recognizing specific carbohydrates reversibly without changing its structure. The legume lectins family is the most studied family of plant lectins, in particular the Diocleinae subtribe, which possesses high degree of structural similarity, but variable biological activities. This variability lies in small differences that can be analyzed in studies based on structures. In particular, Dioclea sclerocarpa seed lectin (DSL) presents low ability to relax endothelialized rat aorta in comparison with other Dioclea lectins such as Dioclea violacea (DVL), Dioclea virgata (DvirL) and Dioclea rostrata (DRL). The DSL relaxation mechanism relies on nitric oxide production and carbohydrate recognition domain (CRD). This feature can be explained by structural differences, since DSL has a carbohydrate recognition domain design less favorable. In addition, the presence of a glutamate residue at position 205 proved to be a decisive factor for the low relaxant effect of Dioclea lectins.
[Mh] Termos MeSH primário: Dioclea/química
Lectinas de Plantas/química
Lectinas de Plantas/farmacologia
Vasodilatadores/química
Vasodilatadores/farmacologia
[Mh] Termos MeSH secundário: Animais
Aorta/efeitos dos fármacos
Sítios de Ligação
Carboidratos/química
Modelos Moleculares
Lectinas de Plantas/isolamento & purificação
Ligação Proteica
Conformação Proteica
Ratos
Relação Estrutura-Atividade
Vasodilatadores/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Plant Lectins); 0 (Vasodilator Agents)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151027
[St] Status:MEDLINE


  5 / 27 MEDLINE  
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[PMID]:26464029
[Au] Autor:Pinto-Junior VR; Correia JL; Pereira RI; Pereira-Junior FN; Santiago MQ; Osterne VJ; Madeira JC; Cajazeiras JB; Nagano CS; Delatorre P; Assreuy AM; Nascimento KS; Cavada BS
[Ad] Endereço:Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
[Ti] Título:Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity.
[So] Source:J Mol Recognit;29(4):134-41, 2016 Apr.
[Is] ISSN:1099-1352
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α-methyl-D-mannoside, D-mannose, and D-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, ß, and γ) with masses of 25,562, 12,874, and 12,706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp.
[Mh] Termos MeSH primário: Dioclea/química
Edema/induzido quimicamente
Manose/farmacologia
Lectinas de Plantas/isolamento & purificação
Lectinas de Plantas/farmacologia
[Mh] Termos MeSH secundário: Animais
Cromatografia de Afinidade
Eritrócitos/efeitos dos fármacos
Hemaglutinação/efeitos dos fármacos
Mediadores da Inflamação/isolamento & purificação
Mediadores da Inflamação/farmacologia
Camundongos
Lectinas de Plantas/química
Estrutura Secundária de Proteína
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Inflammation Mediators); 0 (Plant Lectins); PHA4727WTP (Mannose)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151015
[St] Status:MEDLINE
[do] DOI:10.1002/jmr.2512


  6 / 27 MEDLINE  
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[PMID]:26166677
[Au] Autor:Oliveira CT; Kunz D; Silva CP; Macedo ML
[Ad] Endereço:Programa de Pós-Graduação em Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil; Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, SBBq, Brazil.
[Ti] Título:Entomotoxic properties of Dioclea violacea lectin and its effects on digestive enzymes of Anagasta kuehniella (Lepidoptera).
[So] Source:J Insect Physiol;81:81-9, 2015 Oct.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Entomotoxic plant lectins have been extensively studied in the past two decades, yet the exact mechanisms underlying their toxic effects remain unknown. This study investigated the effects of Dioclea violacea lectin (DVL) on larval development in Anagasta kuehniella. Chronic exposure of larvae (from neonates to the fourth instar) demonstrated that DVL interfered with larval growth, retarding development and decreasing larval mass without affecting survival. DVL decreased trypsin-like, chymotrypsin-like, and α-amylase activities and proved resistant to proteolysis by midgut proteases up to 24h. Shorter exposures to dietary DVL had no effect on midgut enzyme activity. Feeding fourth-instar larvae with fluorescently-labeled DVL revealed lectin binding to the peritrophic membrane.
[Mh] Termos MeSH primário: Dioclea
Mariposas/enzimologia
Lectinas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Animais
Peso Corporal
Trato Gastrointestinal/enzimologia
Larva/efeitos dos fármacos
Larva/enzimologia
Larva/crescimento & desenvolvimento
Mariposas/efeitos dos fármacos
Mariposas/crescimento & desenvolvimento
Lectinas de Plantas/toxicidade
Proteólise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Lectins)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:150919
[Lr] Data última revisão:
150919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150714
[St] Status:MEDLINE


  7 / 27 MEDLINE  
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[PMID]:25934529
[Au] Autor:de Queiroz LP; Pastore JF; Cardoso D; Snak C; de C Lima AL; Gagnon E; Vatanparast M; Holland AE; Egan AN
[Ad] Endereço:Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Av. Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, Bahia, Brazil. Electronic address: luciano.paganucci@gmail.com.
[Ti] Título:A multilocus phylogenetic analysis reveals the monophyly of a recircumscribed papilionoid legume tribe Diocleae with well-supported generic relationships.
[So] Source:Mol Phylogenet Evol;90:1-19, 2015 Sep.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deciphering the phylogenetic relationships within the species-rich Millettioid clade has persisted as one of the major challenges in the systematics and evolutionary history of papilionoid legumes (Leguminosae, Papilionoideae). Historically, the predominantly neotropical lianas of subtribe Diocleinae in the Millettioid legumes have been taxonomically tangled together with the largely heterogeneous tribe Phaseoleae. This work presents a comprehensive molecular phylogenetic analysis based on nuclear and chloroplast markers and includes all genera ever referred to Diocleae except for the monospecific Philippine Luzonia, resolving several key generic relationships within the Millettioid legumes. The first of two separate analyses includes 310 matK accessions and strongly supports the reestablishment of tribe Diocleae as a branch of the Millettioid clade. This work sheds greater light on the higher-level phylogeny of Diocleae and allows the recognition of three major lineages: the Canavalia, Dioclea, and Galactia clades. The second set of phylogenetic analyses utilized nuclear (ITS/5.8S and ETS) and plastid (matK and trnT-Y) DNA sequences to reveal (i) the monophyly of Canavalia and Cleobulia; (ii) the monophyly of Bionia with the exclusion of Bionia bella; (iii) the paraphyly of Dioclea with respect to Cleobulia, Cymbosema, and Macropsychanthus; (iv) the paraphyly of Cratylia with respect to the broadly polyphyletic Camptosema; and (v) the polyphyly of Galactia with species scattered widely across the tree.
[Mh] Termos MeSH primário: Dioclea/classificação
Filogenia
[Mh] Termos MeSH secundário: Teorema de Bayes
Evolução Biológica
Núcleo Celular/genética
Cloroplastos/genética
DNA de Plantas/análise
DNA de Plantas/genética
Flores/genética
Seres Humanos
Plastídeos/genética
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150708
[Lr] Data última revisão:
150708
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150503
[St] Status:MEDLINE


  8 / 27 MEDLINE  
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PubMed Central Texto completo
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[PMID]:25431778
[Au] Autor:Monteiro Tínel JM; Benevides MF; Frutuoso MS; Rocha CF; Arruda FV; Vasconcelos MA; Pereira-Junior FN; Cajazeiras JB; do Nascimento KS; Martins JL; Teixeira EH; Cavada BS; dos Santos RP; Lima Pompeu MM
[Ad] Endereço:Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
[Ti] Título:A lectin from Dioclea violacea Interacts with midgut surface of Lutzomyia migonei, unlike its homologues, Cratylia floribunda lectin and Canavalia gladiata lectin.
[So] Source:ScientificWorldJournal;2014:239208, 2014.
[Is] ISSN:1537-744X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania.
[Mh] Termos MeSH primário: Canavalia
Dioclea
Absorção Gastrointestinal/fisiologia
Lectinas de Plantas/metabolismo
Psychodidae
[Mh] Termos MeSH secundário: Animais
Lectinas/isolamento & purificação
Lectinas/metabolismo
Lectinas de Plantas/isolamento & purificação
Sementes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins); 0 (Plant Lectins); 0 (lectin, Canavalia)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141129
[St] Status:MEDLINE
[do] DOI:10.1155/2014/239208


  9 / 27 MEDLINE  
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[PMID]:24446543
[Au] Autor:Leisinger AK; Janzen DH; Hallwachs W; Igloi GL
[Ti] Título:Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica.
[So] Source:Insect Biochem Mol Biol;43(12):1172-80, 2013 Dec.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:L-canavanine, the toxic guanidinooxy analogue of L-arginine, is the product of plant secondary metabolism. The need for a detoxifying mechanism for the producer plant is self-evident but the larvae of the bruchid beetle Caryedes brasiliensis, that is itself a non-producer, have specialized in feeding on the Lcanavanine-containing seeds of Dioclea megacarpa. The evolution of a seed predator that can imitate the enzymatic abilities of the host permits us to address the question of whether the same problem of amino acid recognition in two different kingdoms has been solved by the same mechanism. A discriminating arginyl-tRNA synthetase, detected in a crude C. brasiliensis larval extract, was proposed to be responsible for insect's ability to survive the diet of L-canavanine (Rosenthal, G. A., Dahlman, D. L., and Janzen, D. H. (1976) A novel means for dealing with L-canavanine, a toxic metabolite. Science 192, 256e258). Since the arginyl-tRNA synthetase of at least three genetic compartments (insect cytoplasmic, insect mitochondrial and insect gut microflora) may participate in conferring L-canavanine resistance, we investigated whether the nuclear-encoded C. brasiliensis mitochondrial arginyl-tRNA synthetase plays a role in this discrimination. Steady state kinetics of the cloned, recombinant enzyme have revealed and quantified an amino acid discriminating potential of the mitochondrial enzyme that is sufficient to account for the overall L-canavanine misincorporation rate observed in vivo. As in the cytoplasmic enzyme of the L-canavanine producer plant, the mitochondrial arginyl-tRNA synthetases from a specialist seed predator relies on a kinetic discrimination that prevents L-canavanine misincorporation into proteins.
[Mh] Termos MeSH primário: Arginina-tRNA Ligase/metabolismo
Canavanina/toxicidade
Núcleo Celular/metabolismo
Mitocôndrias/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/genética
Animais
Arginina-tRNA Ligase/química
Arginina-tRNA Ligase/genética
Canavanina/química
Núcleo Celular/genética
Coleópteros/efeitos dos fármacos
Coleópteros/enzimologia
Coleópteros/metabolismo
Costa Rica
Dioclea/química
Cinética
Larva/efeitos dos fármacos
Larva/crescimento & desenvolvimento
Mitocôndrias/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 3HZV514J4B (Canavanine); EC 6.1.1.19 (Arginine-tRNA Ligase)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140122
[St] Status:MEDLINE


  10 / 27 MEDLINE  
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[PMID]:24008245
[Au] Autor:Pinto-Júnior VR; de Santiago MQ; Osterne VJ; Correia JL; Pereira-Júnior FN; Cajazeiras JB; de Vasconcelos MA; Teixeira EH; do Nascimento AS; Miguel TB; Miguel Ede C; Sampaio AH; do Nascimento KS; Nagano CS; Cavada BS
[Ad] Endereço:Laboratory of Biologically Active Molecules (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Av. Humberto Monte s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza-CE 60440-970, Brazil. juniorreis4@hotmail.com
[Ti] Título:Purification, partial characterization and immobilization of a mannose-specific lectin from seeds of Dioclea lasiophylla mart.
[So] Source:Molecules;18(9):10857-69, 2013 Sep 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-D-mannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, ß and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,588 ± 1 Da, respectively, and has no disulfide bonds. The hemagglutinating activity of DlyL was optimal in pH 8.0, stable at a temperature of 70 °C and decreased in EDTA solution, indicating that lectin activity is dependent on divalent metals. DlyL exhibited low toxicity on Artemia sp. nauplii, but this effect was dependent on the concentration of lectin in solution. DlyL immobilized on cyanogen bromide-activated Sepharose® 4B bound 0.917 mg of ovalbumin per cycle, showing the ability to become a tool for glycoproteomics studies.
[Mh] Termos MeSH primário: Dioclea/química
Hemaglutininas/farmacologia
Lectinas de Ligação a Manose/farmacologia
Extratos Vegetais/farmacologia
Sementes/química
[Mh] Termos MeSH secundário: Animais
Artemia
Quelantes/química
Cromatografia de Afinidade
Ácido Edético/química
Eritrócitos/efeitos dos fármacos
Hemaglutinação
Hemaglutininas/química
Hemaglutininas/isolamento & purificação
Concentração de Íons de Hidrogênio
Dose Letal Mediana
Lectinas de Ligação a Manose/química
Lectinas de Ligação a Manose/isolamento & purificação
Ovalbumina/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Ligação Proteica
Coelhos
Sefarose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Hemagglutinins); 0 (Mannose-Binding Lectins); 0 (Plant Extracts); 9006-59-1 (Ovalbumin); 9012-36-6 (Sepharose); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:130906
[Lr] Data última revisão:
130906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130907
[St] Status:MEDLINE
[do] DOI:10.3390/molecules180910857



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