[PMID]: | 29246138 |
[Au] Autor: | Ayeka PA; Bian Y; Githaiga PM; Zhao Y |
[Ad] Endereço: | International College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, 312 Anshan Western Road, Nankai District, Tianjin, 300193, People's Republic of China. |
[Ti] Título: | The immunomodulatory activities of licorice polysaccharides (Glycyrrhiza uralensis Fisch.) in CT 26 tumor-bearing mice. |
[So] Source: | BMC Complement Altern Med;17(1):536, 2017 Dec 15. |
[Is] ISSN: | 1472-6882 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: The increasing use of complementary and alternative medicine (CAM) has kindled the need for scientific evaluation of the mechanism of action of CAMs. Although, licorice, a common ingredient in many Traditional Chinese medicine (TCM) has attracted great attention for its antitumor and immunomodulatory activities, the mechanism of action of its polysaccharides is still unclear. Here we report the immunomodulatory activity of licorice polysaccharides in vivo. METHODS: The differential anticancer activities of licorice polysaccharides by tumorigenesis and immunomodulation was evaluated in vivo. Six weeks old, 120 CT-26 tumor bearing BALB/c mice, weighing 20 ± 2 g were used. They were randomly divided into six groups, three groups receiving high molecular weight (fraction A), low molecular weight (fraction B) polysaccharides and crude extract (fraction C); positive, negative and normal groups receiving cytoxin, saline and normal diet respectively. Weight of mice and tumors was determined and tumorigenicity assay calculated to determine the anticancer effects. Immunomodulatory potential was determined by immune organ indices, immune cell population and serum cytokine levels using immune organ weight and index, flow cytometry and cytokine/chemokine bead panel kit respectively. RESULTS: Licorice polysaccharides exhibited immunomodulatory activities in CT 26 tumor bearing BALB/c mice. The polysaccharides significantly suppressed tumor growth and increased immune organ index. Furthermore, the immunomodulatory effect was evident with activation of CD4 and CD8 immune cells population. The polysaccharides also affected the production of various cytokines, by increasing IL 2, IL 6, IL 7 levels and a decreasing TNFα levels. CONCLUSION: In summary, licorice polysaccharide especially of low molecular weight exhibit anticancer and immunomodulatory activities by suppressing tumor growth and improving general health of mice. They also augment the thymus/spleen index and population of T lymphocytes. Furthermore, the polysaccharides enhance the levels of serum antitumor cytokines, IL 2, IL 6 and IL 7 while decreasing pro-tumor cytokine TNFα. |
[Mh] Termos MeSH primário: |
Antineoplásicos/farmacologia Glycyrrhiza uralensis Fatores Imunológicos/farmacologia Extratos Vegetais/farmacologia Polissacarídeos/farmacologia
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[Mh] Termos MeSH secundário: |
Animais Antineoplásicos/química Linhagem Celular Tumoral Citocinas/sangue Fatores Imunológicos/química Camundongos Camundongos Endogâmicos BALB C Neoplasias Experimentais Extratos Vegetais/química Polissacarídeos/química Baço/química Baço/efeitos dos fármacos Timo/química Timo/efeitos dos fármacos
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antineoplastic Agents); 0 (Cytokines); 0 (Immunologic Factors); 0 (Plant Extracts); 0 (Polysaccharides) |
[Em] Mês de entrada: | 1801 |
[Cu] Atualização por classe: | 180108 |
[Lr] Data última revisão:
| 180108 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171217 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1186/s12906-017-2030-7 |
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