Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.356 [Categoria DeCS]
Referências encontradas : 9 [refinar]
Mostrando: 1 .. 9   no formato [Detalhado]

página 1 de 1

  1 / 9 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:22163018
[Au] Autor:Staats M; Cuenca A; Richardson JE; Vrielink-van Ginkel R; Petersen G; Seberg O; Bakker FT
[Ad] Endereço:Biosystematics Group, Wageningen University, Wageningen, The Netherlands.
[Ti] Título:DNA damage in plant herbarium tissue.
[So] Source:PLoS One;6(12):e28448, 2011.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dried plant herbarium specimens are potentially a valuable source of DNA. Efforts to obtain genetic information from this source are often hindered by an inability to obtain amplifiable DNA as herbarium DNA is typically highly degraded. DNA post-mortem damage may not only reduce the number of amplifiable template molecules, but may also lead to the generation of erroneous sequence information. A qualitative and quantitative assessment of DNA post-mortem damage is essential to determine the accuracy of molecular data from herbarium specimens. In this study we present an assessment of DNA damage as miscoding lesions in herbarium specimens using 454-sequencing of amplicons derived from plastid, mitochondrial, and nuclear DNA. In addition, we assess DNA degradation as a result of strand breaks and other types of polymerase non-bypassable damage by quantitative real-time PCR. Comparing four pairs of fresh and herbarium specimens of the same individuals we quantitatively assess post-mortem DNA damage, directly after specimen preparation, as well as after long-term herbarium storage. After specimen preparation we estimate the proportion of gene copy numbers of plastid, mitochondrial, and nuclear DNA to be 2.4-3.8% of fresh control DNA and 1.0-1.3% after long-term herbarium storage, indicating that nearly all DNA damage occurs on specimen preparation. In addition, there is no evidence of preferential degradation of organelle versus nuclear genomes. Increased levels of C→T/G→A transitions were observed in old herbarium plastid DNA, representing 21.8% of observed miscoding lesions. We interpret this type of post-mortem DNA damage-derived modification to have arisen from the hydrolytic deamination of cytosine during long-term herbarium storage. Our results suggest that reliable sequence data can be obtained from herbarium specimens.
[Mh] Termos MeSH primário: Dano ao DNA
DNA de Plantas/genética
[Mh] Termos MeSH secundário: Núcleo Celular/metabolismo
Fragmentação do DNA
DNA Mitocondrial/genética
Etiquetas de Sequências Expressas
Ginkgo biloba/genética
Laburnum/genética
Mitocôndrias/genética
Modelos Genéticos
Modelos Estatísticos
Nucleotídeos/genética
Plantas/genética
Plastídeos/metabolismo
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Análise de Sequência de DNA
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Plant); 0 (Nucleotides)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:150129
[Lr] Data última revisão:
150129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0028448


  2 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:21385905
[Au] Autor:Thompson-Evans TP; Glover MP; Walker N
[Ad] Endereço:Centre for Tobacco Control Research, Social and Community Health, School of Population Health, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
[Ti] Título:Cytisine's potential to be used as a traditional healing method to help indigenous people stop smoking: a qualitative study with Maori.
[So] Source:Nicotine Tob Res;13(5):353-60, 2011 May.
[Is] ISSN:1469-994X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Maori experience a disproportionate amount of smoking-related harm (46% of adult Maori smoke). Effective cessation treatments that are both accessible and attractive to Maori are urgently needed. Cytisine (a plant extract found in Golden Rain [Cytisus laburnum L.] and the New Zealand Kowhai [Sophora tetraptera L.] has a similar molecular makeup to nicotine, has been used successfully as a cessation product in central and eastern Europe and central Asia for many years, and is low priced. Recent reviews have found that cytisine is twice as effective as a placebo for smoking cessation. This study aimed to explore cytisine's potential as a 'rongoa Maori' (traditional Maori remedy) and its attractiveness to Maori smokers compared with other cessation products. METHODS: Maori that smoked were interviewed in two focus groups and eight individual semi-structured interviews. Two key informants were interviewed also. RESULTS: Barriers to using cessation products were financial and effort cost, pervasive smoking among family and peers, environments permissive of smoking, and perceived cultural inappropriateness of treatments. Participants were very interested in cytisine, supported the idea that it would be acceptable to package it as a rongoa Maori, and all wanted to use it. Named appropriately, packaged and promoted as a Maori cessation product, participants thought cytisine would contribute to the restoration of Maori identity and traditional beliefs and practices in addition to reducing smoking. CONCLUSIONS: Presented as a rongoa Maori, cytisine would likely be more attractive to Maori than currently available cessation products. Confirmation of efficacy and safety will be needed before promotion of the product could occur.
[Mh] Termos MeSH primário: Alcaloides/uso terapêutico
Agonistas Nicotínicos/uso terapêutico
Grupo com Ancestrais Oceânicos
Abandono do Hábito de Fumar/métodos
Fumar/etnologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Azocinas/uso terapêutico
Feminino
Seres Humanos
Laburnum/química
Masculino
Nova Zelândia
Extratos Vegetais/uso terapêutico
Quinolizinas/uso terapêutico
Fumar/psicologia
Prevenção do Hábito de Fumar
Sophora/química
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Azocines); 0 (Nicotinic Agonists); 0 (Plant Extracts); 0 (Quinolizines); 53S5U404NU (cytisine)
[Em] Mês de entrada:1108
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110310
[St] Status:MEDLINE
[do] DOI:10.1093/ntr/ntr002


  3 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:19217730
[Au] Autor:Musshoff F; Madea B
[Ad] Endereço:Institute of Forensic Medicine, Stiftsplatz 12, 53111 Bonn, Germany. f.musshoff@uni-bonn.de
[Ti] Título:Fatal cytisine intoxication and analysis of biological samples with LC-MS/MS.
[So] Source:Forensic Sci Int;186(1-3):e1-4, 2009 Apr 15.
[Is] ISSN:1872-6283
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:We report about a fatal cytisine intoxication in a 20-year-old man who, according to his mother, had drunken tea prepared from plant material of Laburnum anagyroides with the toxic pyridine-like alkaloid as ingredient, which exhibits pharmacological effects similar to nicotine. Using a liquid chromatographic-mass spectrometric (LC-MS) procedure cytisine was quantified in post-mortem specimens. By exclusion of other causes of death an intoxication was determined as the cause of death with respiratory failure as the pathophysiological mechanism.
[Mh] Termos MeSH primário: Alcaloides/análise
Alcaloides/envenenamento
[Mh] Termos MeSH secundário: Azocinas/análise
Azocinas/envenenamento
Bebidas
Bile/química
Química Encefálica
Cromatografia Líquida
Toxicologia Forense
Conteúdo Gastrointestinal/química
Seres Humanos
Rim/química
Laburnum/efeitos adversos
Fígado/química
Masculino
Espectrometria de Massas
Quinolizinas/análise
Quinolizinas/envenenamento
Insuficiência Respiratória/induzido quimicamente
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Azocines); 0 (Quinolizines); 53S5U404NU (cytisine)
[Em] Mês de entrada:0907
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090217
[St] Status:MEDLINE
[do] DOI:10.1016/j.forsciint.2009.01.011


  4 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:19164465
[Au] Autor:Mineur YS; Eibl C; Young G; Kochevar C; Papke RL; Gündisch D; Picciotto MR
[Ad] Endereço:Department of Psychiatry, Yale School of Medicine, New Haven, CT 06508, USA.
[Ti] Título:Cytisine-based nicotinic partial agonists as novel antidepressant compounds.
[So] Source:J Pharmacol Exp Ther;329(1):377-86, 2009 Apr.
[Is] ISSN:1521-0103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nicotine and other nicotinic agents are thought to regulate mood in human subjects and have antidepressant-like properties in animal models. Recent studies have demonstrated that blockade of nicotinic acetylcholine receptors (nAChRs) including those containing the beta2 subunit (beta2(*)), results in antidepressant-like effects. Previous studies have shown that cytisine, a partial agonist at alpha4/beta2(*) nAChRs, and a full agonist at alpha3/beta4(*) and alpha7 nAChRs, has antidepressant-like properties in several rodent models of antidepressant efficacy; however, it is not clear whether more selective partial agonists will also be effective in these models. We tested cytisine and two derivatives, 5-bromo-cytisine (5-Br-Cyt) and 3-(pyridin-3'-yl)-cytisine (3-pyr-Cyt) for their ability to act as a partial agonist of different nAChR subtypes and to show antidepressant-like activity in C57/BL6 mice in the tail suspension, the forced-swim, and the novelty-suppressed feeding tests. 3-pyr-Cyt was a partial agonist with very low efficacy at alpha4/beta2(*) nAChRS but had no agonist effects at other nAChRs normally targeted by cytisine, and it was effective in mouse models of antidepressant efficacy. Animals showed dose-dependent antidepressant-like effects in all three behavioral paradigms. 5-Br-Cyt was not effective in behavioral tests when administered peripherally, probably because of its inability to penetrate the blood-brain barrier, because it efficiently reduced immobility in the tail suspension test when administered intraventricularly. These results suggest that novel nicotinic partial agonists may provide new possibilities for development of drugs to treat mood disorders.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Antidepressivos/farmacologia
Agonistas Nicotínicos/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/química
Animais
Antidepressivos/química
Azocinas/química
Azocinas/farmacologia
Clonagem Molecular
Interpretação Estatística de Dados
Eletrofisiologia
Meio Ambiente
Comportamento Alimentar/efeitos dos fármacos
Elevação dos Membros Posteriores/psicologia
Laburnum/química
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Atividade Motora/efeitos dos fármacos
Agonistas Nicotínicos/química
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
Técnicas de Patch-Clamp
Quinolizinas/química
Quinolizinas/farmacologia
Receptores Colinérgicos/efeitos dos fármacos
Receptores Colinérgicos/genética
Natação/psicologia
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antidepressive Agents); 0 (Azocines); 0 (Nicotinic Agonists); 0 (Quinolizines); 0 (Receptors, Cholinergic); 53S5U404NU (cytisine)
[Em] Mês de entrada:0904
[Cu] Atualização por classe:161122
[Lr] Data última revisão:
161122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090124
[St] Status:MEDLINE
[do] DOI:10.1124/jpet.108.149609


  5 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:18803865
[Au] Autor:Adedapo AA; Jimoh FO; Koduru S; Afolayan AJ; Masika PJ
[Ad] Endereço:Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria. adedapo3a@yahoo.co.uk
[Ti] Título:Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea.
[So] Source:BMC Complement Altern Med;8:53, 2008 Sep 20.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In South Africa, Calpurnia aurea (Ait.) Benth is used to destroy lice and to relieve itches, to destroy maggots and to treat allergic rashes, particularly those caused by caterpillars. Antioxidants play an important role protecting against damage by reactive oxygen species. Plants containing flavonoids have been reported to possess strong antioxidant properties. METHODS: The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of Calpurnia aurea were evaluated using in vitro standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Calpurnia aurea were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant. RESULTS: The results from this study showed that the antioxidant activities of the stem extract of Calpurnia aurea as determined by the total phenol, flavonoids, and FRAP methods were higher than that of the leaves. On the other hand, the leaf extract of the plant has higher level of total flavonols and proanthocyanidins. The leaf extract also has higher radical scavenging activity as shown in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), and 2,2¿-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. The leaf extract showed activity against seven of the bacterial organisms. CONCLUSION: The results from this study indicate that the leaves and stem extracts of Calpurnia aurea possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of Calpurnia aurea are not as effective as the standard drugs- Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. Calpurnia aurea may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Depuradores de Radicais Livres/farmacologia
Laburnum/química
Fenol/farmacologia
Folhas de Planta/química
Caules de Planta/química
[Mh] Termos MeSH secundário: Antibacterianos/análise
Antioxidantes/análise
Benzotiazóis/análise
Compostos de Bifenilo
Cromatografia Líquida de Alta Pressão
Depuradores de Radicais Livres/análise
Seres Humanos
Fenol/análise
Picratos/análise
Extratos Vegetais/química
Ácidos Sulfônicos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Benzothiazoles); 0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Picrates); 0 (Plant Extracts); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); 339NCG44TV (Phenol); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:0811
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080923
[St] Status:MEDLINE
[do] DOI:10.1186/1472-6882-8-53


  6 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:17375674
[Au] Autor:Piskarev VE; Bushueva TL; Iamskov IA
[Ti] Título:[Interaction of the Laburnum anagyroides lectin with fucoantigens].
[So] Source:Bioorg Khim;33(1):182-6, 2007 Jan-Feb.
[Is] ISSN:0132-3423
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:We studied interaction of the lectin from the bark of Golden Rain shrub (Laburnum anagyroides, LABA) with a number of basic fucose-containing carbohydrate antigens by changes in its tryptophan fluorescence. The strongest LABA binding was observed for the trisaccharide H of type 6 [alpha-L-Fucp-(1-2)-beta-D-Galp-(1-4)-D-Glc, Ka= 4.2 x 10(3) M(-1)]. The following antigens were bound with a weaker affinity: H-disaccharide alpha-L-Fucp-(1-2)-D-Gal, a glucoanalogue of tetrasaccharide Ley alpha-L-Fucp-(1-2)-beta-D-Galp-(1-4)-[alpha-L-Fucp-(1-3)]-D-Glc, and 6-fucosyl-N-acetylglucosamine, a fragment of core of the N-glycans family (Ka 1.1-1.7 x 10(3) M(-1)). The lowest binding was observed for L-fucose (Ka = 2.7 x 10(2) M-1) and trisaccharide Lea, (3-Galp-(1-3)-[a-L-Fucp-(1-4)]-GlcNAc (Ka = 6.4 x 10(2) M(-1)). The Lea, Lea, and Lex pentasaccharides and Leb hexasaccharide were not bound to LABA.
[Mh] Termos MeSH primário: Antígenos/química
Fucose/química
Laburnum/química
Lectinas/química
[Mh] Termos MeSH secundário: Fluorescência
Lectinas/isolamento & purificação
Oligossacarídeos/química
Triptofano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Lectins); 0 (Oligosaccharides); 0 (fucose-binding lectin); 28RYY2IV3F (Fucose); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070323
[St] Status:MEDLINE


  7 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:17375661
[Au] Autor:Piskarev VE; Iamskov IA
[Ti] Título:[Structure of carbohydrate chains of fucolectin from the bark of golden rain shrub Laburnum anagyroides].
[So] Source:Bioorg Khim;33(1):69-73, 2007 Jan-Feb.
[Is] ISSN:0132-3423
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1XyllGlcNAc2 (M2FX), Man3XyllGlcNAc2 (M3X), Man3FuclXyllGlcNAc2 (M3FX), and Man3XyllFucIGlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1 : 8 : 1 : 3 ratio.
[Mh] Termos MeSH primário: Laburnum/química
Lectinas/química
Oligossacarídeos/química
[Mh] Termos MeSH secundário: Sequência de Carboidratos
Lectinas/isolamento & purificação
Dados de Sequência Molecular
Oligossacarídeos/isolamento & purificação
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lectins); 0 (Oligosaccharides); 0 (Polysaccharides); 0 (fucose-binding lectin)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:080819
[Lr] Data última revisão:
080819
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070323
[St] Status:MEDLINE


  8 / 9 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:14994587
[Au] Autor:Nozdrachev AD; Akkuratov EG; Fateev MM
[Ad] Endereço:Department of General Physiology, St. Petersburg State University.
[Ti] Título:[Populations of afferent neurons in the sensory ganglia detected using lectin from Laburnum anagyroides].
[Ti] Título:Populiatsii afferentnykh neironov v chuvstvitel'nykh uzlakh, vyiavlennye s ispol'zovaniem lektina bobovnika anagirolistnogo..
[So] Source:Morfologiia;124(6):38-40, 2003.
[Is] ISSN:1026-3543
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The purpose of the present research was the study of afferent neuron subpopulations in vagal caudal ganglia and trigeminal ganglion of adult albino rats using a conjugate of fucose-specific Laburnum anagyroides lectin (LAL) with peroxidase. Histochemical preparations obtained were examined using computer videoanalyzer with the determination of dimensions of afferent neurons and integral optical density (IOD) of their cytoplasm. In the ganglia studied LAL was found to bind to the majority (more than 98%) of neurons. Reaction product was demonstrated in the perikaryon either as discrete granules (Nissl body-type), or as a uniform precipitate. Nucleoplasm in most of neurons remained lightly stained. Determination of IOD of neuronal cytoplasm in various ganglia demonstrated significant differences in degree of LAL accumulation. Analysis of interrelation between neuronal size and IOD permitted to establish non-linear correlation of metric and optical parameters and to detect subpopulations of cells in the sensory ganglia, which were stained with LAL most intensely. Functional specialization of these cells remains to be determined. Thus, a combined application of lectin-histochemical method with a computer videoanalysis of morphological slides enabled the identification neuronal populations and subpopulations in rat afferent ganglia, which are not demonstrated with the standard histological methods.
[Mh] Termos MeSH primário: Laburnum/química
Neurônios Aferentes/citologia
Gânglio Nodoso/citologia
Lectinas de Plantas
Gânglio Trigeminal/citologia
[Mh] Termos MeSH secundário: Animais
Histocitoquímica
Masculino
Gânglio Nodoso/anatomia & histologia
Lectinas de Plantas/isolamento & purificação
Ratos
Gânglio Trigeminal/anatomia & histologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Lectins)
[Em] Mês de entrada:0408
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040305
[St] Status:MEDLINE


  9 / 9 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:14593874
[Au] Autor:Piskarev VE; Lutsik-Kordovskii MD; Piskareva EL; Iamskov IA
[Ad] Endereço:Nesmeyanov Institute of Organoelemental Compounds, Russian Academy of Sciences, Moscow, 119991 Russia. piskarev@ineos.as.ru
[Ti] Título:[Oligosaccharide specificity of the fucolectin from the bark of laburnum Laburnum anagyroides].
[Ti] Título:Oligosakharidnaia spetsifichnost' fukolektina kory bobovnika Laburnum anagyroides..
[So] Source:Prikl Biokhim Mikrobiol;39(5):581-7, 2003 Sep-Oct.
[Is] ISSN:0555-1099
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A comparative study of thin carbohydrate specificity of the lectin from the bark of laburnum Laburnum anagyroides (LABA) and fucolectin from asparagus pea Tetragonolobus purpureus (TPA) was performed using inhibition of agglutination of the complex formed by H-active neoglycoprotein and nanoparticles of colloidal gold. Both lectins bound most strongly the H type 2 oligosaccharides comprising O-glycanes; however, TPA was almost unable to discriminate between them. LABA bound more weakly the H type 6 trisaccharide (Fuc alpha 1-2Gal beta 1-4Glc) and difucosyllactose (Fuc alpha 1-2Gal beta 1-4[Fuc alpha 1-3]Glc), a glucoanalogue of the Le(y) antigen, and, even more weakly, the Le(a) pentasaccharide lacto-N-fucopentaose II (Gal beta 1-3[Fuc alpha 1-4]GlcNAc beta 1-3Gal beta 1-4Glc). However, LABA did not bind the antigens Le(b), Le(c), and Le(d), very poorly interacted with the terminal Le(x), and somewhat more strongly bound the internal Le(x). The lectin also had a hydrophobic binding site. Both lectins exhibited a cluster effect with polymeric ligands (neoglycoproteins).
[Mh] Termos MeSH primário: Laburnum/química
Lectinas/química
Oligossacarídeos/química
Casca de Planta/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Sequência de Carboidratos
Glicoproteínas/química
Laburnum/metabolismo
Dados de Sequência Molecular
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (Lectins); 0 (Oligosaccharides); 0 (fucose-binding lectin)
[Em] Mês de entrada:0406
[Cu] Atualização por classe:080819
[Lr] Data última revisão:
080819
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:031105
[St] Status:MEDLINE



página 1 de 1
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde