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Pesquisa : B01.650.940.800.575.912.250.401.571 [Categoria DeCS]
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[PMID]:29284255
[Au] Autor:Danciu C; Pavel IZ; Babuta R; Ersilia A; Pop G; Soica C; Dehelean C; Radulov I; Oana S
[Ad] Endereço:University of Medicine and Pharmacy "Victor Babes", Timisoara, Romania. corina.danciu@umft.ro.
[Ti] Título:Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania.
[So] Source:Ann Agric Environ Med;24(4):726-731, 2017 Dec 23.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most studied species due to their intense culinary use and potential biological activity. The intention of the study was to characterize total phenolic content, perform FTIR analysis, and antiproliferative effects against A375 human melanoma cells extracts obtained from germinated and ungerminated seeds from Lupinus albus L. and Lupinus angustifolius L. MATERIAL AND METHODS: Total phenolic content was assessed using the Folin-Ciocalteu colorimetric method. FTIR spectra were carried out by a Shimadzu Prestige-21 spectrometer in the range 400-4000 cm-1, using KBr pellets and resolution of 4 cm-1. Antiproliferative capacity was screened by employing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and scratch assay methods. RESULTS: The study showed increased values corresponding to total phenolic content for the germinated extracts. FTIR spectra confirmed the presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids). All tested extracts showed weak antiproliferative potential against A375 human melanoma cells. CONCLUSIONS: Germination increased the amount of bioactive compounds in the seeds of the two studied species of lupin. FTIR analyses provided an important fingerprint of the chemical composition.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
Lupinus/química
Fenóis/química
Fenóis/farmacologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Germinação
Seres Humanos
Lupinus/crescimento & desenvolvimento
Romênia
Sementes/química
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


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[PMID]:29190792
[Au] Autor:Atnaf M; Yao N; Martina K; Dagne K; Wegary D; Tesfaye K
[Ad] Endereço:Pawe Research Center, Ethiopian Institute of Agricultural Research, Pawe, Ethiopia.
[Ti] Título:Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation.
[So] Source:PLoS One;12(11):e0188696, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Variação Genética
Lupinus/genética
[Mh] Termos MeSH secundário: Etiópia
Genes de Plantas
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188696


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[PMID]:28863333
[Au] Autor:Mane S; Bringans S; Johnson S; Pareek V; Utikar R
[Ad] Endereço:Department of Chemical Engineering, Curtin University, Perth, WA, 6845, Australia.
[Ti] Título:Reverse phase HPLC method for detection and quantification of lupin seed γ-conglutin.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:123-129, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple, selective and accurate reverse phase HPLC method was developed for detection and quantitation of γ-conglutin from lupin seed extract. A linear gradient of water and acetonitrile containing trifluoroacetic acid (TFA) on a reverse phase column (Agilent Zorbax 300SB C-18), with a flow rate of 0.8ml/min was able to produce a sharp and symmetric peak of γ-conglutin with a retention time at 29.16min. The identity of γ-conglutin in the peak was confirmed by mass spectrometry (MS/MS identification) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The data obtained from MS/MS analysis was matched against the specified database to obtain the exact match for the protein of interest. The proposed method was validated in terms of specificity, linearity, sensitivity, precision, recovery and accuracy. The analytical parameters revealed that the validated method was capable of selectively performing a good chromatographic separation of γ-conglutin from the lupin seed extract with no interference of the matrix. The detection and quantitation limit of γ-conglutin were found to be 2.68µg/ml and 8.12µg/ml respectively. The accuracy (precision and recovery) analysis of the method was conducted under repeatable conditions on different days. Intra-day and inter-day precision values less than 0.5% and recovery greater than 97% indicated high precision and accuracy of the method for analysis of γ-conglutin. The method validation findings were reproducible and can be successfully applied for routine analysis of γ-conglutin from lupin seed extract.
[Mh] Termos MeSH primário: Cromatografia de Fase Reversa/métodos
Lupinus/química
Proteínas de Plantas/análise
Sementes/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Limite de Detecção
Modelos Lineares
Proteínas de Plantas/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (conglutin-gamma protein, Lupinus angustifolius)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE


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[PMID]:28640886
[Au] Autor:Susek K; Braszewska-Zalewska A; Bewick AJ; Hasterok R; Schmitz RJ; Naganowska B
[Ad] Endereço:Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
[Ti] Título:Epigenomic diversification within the genus Lupinus.
[So] Source:PLoS One;12(6):e0179821, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deciphering the various chemical modifications of both DNA and the histone compound of chromatin not only leads to a better understanding of the genome-wide organisation of epigenetic landmarks and their impact on gene expression but may also provide some insights into the evolutionary processes. Although both histone modifications and DNA methylation have been widely investigated in various plant genomes, here we present the first study for the genus Lupinus. Lupins, which are members of grain legumes (pulses), are beneficial for food security, nutrition, health and the environment. In order to gain a better understanding of the epigenetic organisation of genomes in lupins we applied the immunostaining of methylated histone H3 and DNA methylation as well as whole-genome bisulfite sequencing. We revealed variations in the patterns of chromatin modifications at the chromosomal level among three crop lupins, i.e. L. angustifolius (2n = 40), L. albus (2n = 50) and L. luteus (2n = 52), and the legume model plant Medicago truncatula (2n = 16). Different chromosomal patterns were found depending on the specific modification, e.g. H3K4me2 was localised in the terminal parts of L. angustifolius and M. truncatula chromosomes, which is in agreement with the results that have been obtained for other species. Interestingly, in L. albus and L. luteus this modification was limited to one arm in the case of all of the chromosomes in the complement. Additionally, H3K9me2 was detected in all of the analysed species except L. luteus. DNA methylation sequencing (CG, CHG and CHH contexts) of aforementioned crop but also wild lupins such as L. cosentinii (2n = 32), L. digitatus (2n = 36), L. micranthus (2n = 52) and L. pilosus (2n = 42) supported the range of interspecific diversity. The examples of epigenetic modifications illustrate the diversity of lupin genomes and could be helpful for elucidating further epigenetic changes in the evolution of the lupin genome.
[Mh] Termos MeSH primário: Epigenômica
Variação Genética
Lupinus/genética
[Mh] Termos MeSH secundário: Metilação de DNA
DNA de Plantas/genética
DNA de Plantas/metabolismo
Genoma de Planta/genética
Histonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Histones)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179821


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[PMID]:28578197
[Au] Autor:Rydzynski D; Piotrowicz-Cieslak AI; Grajek H; Michalczyk DJ
[Ad] Endereço:Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland; Department of Physics and Biophysics, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Oczapowsk
[Ti] Título:Instability of chlorophyll in yellow lupin seedlings grown in soil contaminated with ciprofloxacin and tetracycline.
[So] Source:Chemosphere;184:62-73, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With increasing soil concentrations of ciprofloxacin and tetracycline a decrease of leaf chlorophyll content was observed. Tetracycline was more detrimental than ciprofloxacin. The chlorophyll content in plants growing for ten days on a tetracycline containing soil decreased by 68%. The decrease of chlorophyll concentration was even sharper in new leaves that formed after application of the antibiotic (up to 81% drop). The comparison of absorption spectra of commercial, reagent grade chlorophyll, alone and incubated with antibiotics, has shown that ciprofloxacin and tetracycline can react directly with chlorophyll and decrease its concentration by 47.7% and 48.5%, respectively. The changes in fluorescence spectra confirmed the formation of chlorophyll degradation product. The chlorophyll decay was a second order reaction and depended on antibiotic concentration and duration of exposure. Reaction rate constants differed with antibiotics and their soil concentrations. With increasing contents of antibiotics in soil the constant of chlorophyll degradation rate in lupin plants increased from k = 870 M day for 3 mg ciprofloxacin to k = 2490 M day for 90 mg ciprofloxacin, and in the case of tetracycline the reaction rate constant increased from k = 1330 M day to k = 2910 M day . The sensitivity of chlorophyll to ciprofloxacin and tetracycline was confirmed by determining EC and TU indices.
[Mh] Termos MeSH primário: Antibacterianos/toxicidade
Clorofila/metabolismo
Ciprofloxacino/toxicidade
Lupinus/fisiologia
Poluentes do Solo/toxicidade
Tetraciclina/toxicidade
[Mh] Termos MeSH secundário: Antibacterianos/metabolismo
Fluorescência
Lupinus/efeitos dos fármacos
Lupinus/metabolismo
Folhas de Planta/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
Solo
Poluentes do Solo/metabolismo
Tetraciclina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Soil); 0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 5E8K9I0O4U (Ciprofloxacin); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


  6 / 732 MEDLINE  
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[PMID]:28532146
[Au] Autor:Karnpanit W; Coorey R; Clements J; Benjapong W; Jayasena V
[Ad] Endereço:School of Science and Health, Western Sydney University , Locked Bag 1797, Penrith, New South Wales 2751, Australia.
[Ti] Título:Calcium, Iron, and Zinc Bioaccessibilities of Australian Sweet Lupin (Lupinus angustifolius L.) Cultivars.
[So] Source:J Agric Food Chem;65(23):4722-4727, 2017 Jun 14.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we aimed to determine the effect of the cultivar and dehulling on calcium, iron, and zinc bioaccessibilities of Australian sweet lupin (ASL). Ten ASL cultivars grown in 2011, 2012, and 2013 in Western Australia were used for the study. The bioaccessibilities of calcium, iron, and zinc in whole seed and dehulled lupin samples were determined using a dialysability method. The cultivar had significant effects on calcium, iron, and zinc contents and their bioaccessibilities. Average bioaccessibilities of 6% for calcium, 17% for iron, and 9% for zinc were found for whole seeds. Dehulled ASL had average calcium, iron, and zinc bioaccessibilities of 11%, 21%, and 12%, respectively. Compared to some other pulses, ASL had better iron bioaccessibility and poorer calcium and zinc bioaccessibilities. Dehulling increased calcium bioaccessibilities of almost all lupin cultivars. The effect of dehulling on iron and zinc bioaccessibilities depends on the ASL cultivar.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Ferro/metabolismo
Lupinus/metabolismo
Zinco/metabolismo
[Mh] Termos MeSH secundário: Austrália
Cálcio/análise
Ferro/análise
Lupinus/química
Lupinus/classificação
Sementes/química
Sementes/classificação
Sementes/metabolismo
Zinco/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
E1UOL152H7 (Iron); J41CSQ7QDS (Zinc); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00445


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[PMID]:28505579
[Au] Autor:Fresno T; Peñalosa JM; Santner J; Puschenreiter M; Moreno-Jiménez E
[Ad] Endereço:Department of Agricultural Chemistry and Food Sciences, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain. Electronic address: teresa.fresno@uam.es.
[Ti] Título:Effect of Lupinus albus L. root activities on As and Cu mobility after addition of iron-based soil amendments.
[So] Source:Chemosphere;182:373-381, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arsenic and Cu mobility was investigated in the rhizosphere of Lupinus albus L. grown in an iron-amended contaminated soil. White lupin was grown in rhizobags in contaminated soil either left untreated or amended with iron sulphate plus lime (Fe + lime) or biochar (Fe + BC). Porewater was monitored in rhizosphere and bulk soil throughout the experiment and the extractable fraction of several elements and As and Cu plant uptake was analysed after 48 days. The distribution of As, Cu, P and Fe in the lupin rhizosphere was evaluated with chemical images obtained by laser ablation-ICP-MS analysis of diffusive gradients in thin films (DGT) gels. The treatments effectively reduced the soluble and extractable As and Cu fractions in the bulk soil, but they did not affect plant uptake. In all cases, soluble As was slightly enhanced in the rhizosphere. This difference was more pronounced in the Fe + lime-treated rhizosphere soil, where an increase of pH as well as extractable As and Fe concentrations were also observed. Chemical imaging of the lupin rhizosphere also showed slightly higher As- and Fe-DGT fluxes around lupin roots grown in the non-amended soil. Our findings indicate As and Fe co-solubilisation by lupin root exudates, likely as a response to P deficiency. Arsenic mobilisation occurred only in the rhizosphere and was not decreased by the amendments.
[Mh] Termos MeSH primário: Ferro/química
Lupinus/química
Solo/química
[Mh] Termos MeSH secundário: Arsênico/farmacocinética
Cobre/farmacocinética
Raízes de Plantas/química
Rizosfera
Poluentes do Solo/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); 789U1901C5 (Copper); E1UOL152H7 (Iron); N712M78A8G (Arsenic)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE


  8 / 732 MEDLINE  
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Rey, Luís
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[PMID]:28505340
[Au] Autor:Msaddak A; Rejili M; Durán D; Rey L; Imperial J; Palacios JM; Ruiz-Argüeso T; Mars M
[Ad] Endereço:Research Unit Biodiversity and Valorization of Arid Areas Bioresources (BVBAA)-Faculty of Sciences of Gabès, Erriadh, Zrig 6072, Tunisia.
[Ti] Título:Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genetic diversity of bacterial populations nodulating Lupinus luteus (yellow lupine) in Northern Tunisia was examined. Phylogenetic analyses of 43 isolates based on recA and gyrB partial sequences grouped them in three clusters, two of which belong to genus Bradyrhizobium (41 isolates) and one, remarkably, to Microvirga (2 isolates), a genus never previously described as microsymbiont of this lupine species. Representatives of the three clusters were analysed in-depth by multilocus sequence analysis of five housekeeping genes (rrs, recA, glnII, gyrB and dnaK). Surprisingly, the Bradyrhizobium cluster with the two isolates LluI4 and LluTb2 may constitute a new species defined by a separate position between Bradyrhizobium manausense and B. denitrificans. A nodC-based phylogeny identified only two groups: one formed by Bradyrhizobium strains included in the symbiovar genistearum and the other by the Microvirga strains. Symbiotic behaviour of representative isolates was tested, and among the seven legumes inoculated only a difference was observed i.e. the Bradyrhizobium strains nodulated Ornithopus compressus unlike the two strains of Microvirga. On the basis of these data, we conclude that L. luteus root nodule symbionts in Northern Tunisia are mostly strains within the B. canariense/B. lupini lineages, and the remaining strains belong to two groups not previously identified as L. luteus endosymbionts: one corresponding to a new clade of Bradyrhizobium and the other to the genus Microvirga.
[Mh] Termos MeSH primário: Bradyrhizobium/genética
Lupinus/microbiologia
Methylobacteriaceae/genética
Nódulos Radiculares de Plantas/microbiologia
Simbiose/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Bradyrhizobium/classificação
Bradyrhizobium/isolamento & purificação
DNA Girase/genética
DNA Bacteriano/genética
Genes Essenciais/genética
Methylobacteriaceae/classificação
Methylobacteriaceae/isolamento & purificação
Tipagem de Sequências Multilocus
Filogenia
Recombinases Rec A/genética
Análise de Sequência de DNA
Solo
Microbiologia do Solo
Simbiose/genética
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (NodD protein, Bacteria); 0 (Soil); EC 2.7.7.- (Rec A Recombinases); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix068


  9 / 732 MEDLINE  
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[PMID]:28338826
[Au] Autor:Keller J; Rousseau-Gueutin M; Martin GE; Morice J; Boutte J; Coissac E; Ourari M; Aïnouche M; Salmon A; Cabello-Hurtado F; Aïnouche A
[Ad] Endereço:UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l'Univers de Rennes), Université de Rennes 1, 35042 Rennes, France.
[Ti] Título:The evolutionary fate of the chloroplast and nuclear rps16 genes as revealed through the sequencing and comparative analyses of four novel legume chloroplast genomes from Lupinus.
[So] Source:DNA Res;24(4):343-358, 2017 Aug 01.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Fabaceae family is considered as a model system for understanding chloroplast genome evolution due to the presence of extensive structural rearrangements, gene losses and localized hypermutable regions. Here, we provide sequences of four chloroplast genomes from the Lupinus genus, belonging to the underinvestigated Genistoid clade. Notably, we found in Lupinus species the functional loss of the essential rps16 gene, which was most likely replaced by the nuclear rps16 gene that encodes chloroplast and mitochondrion targeted RPS16 proteins. To study the evolutionary fate of the rps16 gene, we explored all available plant chloroplast, mitochondrial and nuclear genomes. Whereas no plant mitochondrial genomes carry an rps16 gene, many plants still have a functional nuclear and chloroplast rps16 gene. Ka/Ks ratios revealed that both chloroplast and nuclear rps16 copies were under purifying selection. However, due to the dual targeting of the nuclear rps16 gene product and the absence of a mitochondrial copy, the chloroplast gene may be lost. We also performed comparative analyses of lupine plastomes (SNPs, indels and repeat elements), identified the most variable regions and examined their phylogenetic utility. The markers identified here will help to reveal the evolutionary history of lupines, Genistoids and closely related clades.
[Mh] Termos MeSH primário: Evolução Molecular
Genes de Plantas
Genoma de Cloroplastos
Genoma de Planta
Lupinus/genética
[Mh] Termos MeSH secundário: Núcleo Celular/genética
DNA de Plantas
Genoma Mitocondrial
Mutação INDEL
Filogenia
Polimorfismo de Nucleotídeo Único
Sequências Repetitivas de Ácido Nucleico
Análise de Sequência de DNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsx006


  10 / 732 MEDLINE  
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[PMID]:28325829
[Au] Autor:Pardini EA; Patten MV; Knight TM
[Ad] Endereço:Environmental Studies, Washington University in St. Louis, 1 Brookings Drive, Box 1137, St. Louis, Missouri 63130, USA epardini@wustl.edu.
[Ti] Título:Effects of seed density and proximity to refuge habitat on seed predation rates for a rare and a common species.
[So] Source:Am J Bot;104(3):389-398, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Biotic interactions such as seed predation can play a role in explaining patterns of abundance among plant species. The effect of seed predation will depend on how the strength of predation differs across species and environments, and on the degree to which seed loss at one life-cycle phase increases fitness at another phase. Few studies have simultaneously quantified predispersal and postdispersal predation in co-occurring rare and common congeners, despite the value of estimating both for understanding causes of rarity. METHODS: We quantified predispersal seed predation on the rare, herbaceous species (Fabaceae) and its common, shrubby congener across multiple years in the same community. We experimentally measured postdispersal seed predation at two seed densities and locations near or far from an exotic grass housing high densities of deer mice ( ), their primary, native seed predator. KEY RESULTS: The common had the lowest predispersal seed predation of the two lupine species, potentially because of its height: its high racemes received less predation than those low to the ground. By contrast, the same species experienced higher postdispersal seed predation, and at predators traveled long distances away from refuge habitat to consume their seeds. Across both plant species, mice preferentially predated high-density seed sources. CONCLUSIONS: Our results show differences in the magnitude and direction of seed predation between the species across different life-cycle phases. We demonstrated possible roles of proximity to refuge habitat, seed density, and seed size in these patterns. Congeneric comparisons would benefit from a comprehensive framework that considers seed predation across different life-cycle phases and the environmental context of predation.
[Mh] Termos MeSH primário: Lupinus/fisiologia
Peromyscus/fisiologia
[Mh] Termos MeSH secundário: Animais
California
Ecossistema
Dinâmica Populacional
Dispersão de Sementes
Sementes/fisiologia
Simpatria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600290



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