Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.581 [Categoria DeCS]
Referências encontradas : 59 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 6 ir para página                

  1 / 59 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28651174
[Au] Autor:Belicky S; Cernocká H; Bertok T; Holazova A; Réblová K; Palecek E; Tkac J; Ostatná V
[Ad] Endereço:Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak Republic.
[Ti] Título:Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins.
[So] Source:Bioelectrochemistry;117:89-94, 2017 Oct.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA). Incubation of PSA-modified electrode with specific SNA lectin resulted in an increase of CPS peak H of the complex as compared to this peak of individual PSA. By adjusting polarization current and temperature, PSA-MAA interaction can be either eliminated or distinguished from the more abundant PSA-SNA complex. CPS data were in a good agreement with the data obtained by complementary methods, namely surface plasmon resonance and fluorescent lectin microarray. It can be anticipated that CPS will find application in glycomics and proteomics.
[Mh] Termos MeSH primário: Aglutininas/metabolismo
Condutividade Elétrica
Ácido N-Acetilneuramínico/metabolismo
Antígeno Prostático Específico/química
Antígeno Prostático Específico/metabolismo
[Mh] Termos MeSH secundário: Eletroquímica
Maackia/química
Sambucus nigra/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agglutinins); EC 3.4.21.77 (Prostate-Specific Antigen); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


  2 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28399123
[Au] Autor:Choi IS; Choi BH
[Ad] Endereço:Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
[Ti] Título:The distinct plastid genome structure of Maackia fauriei (Fabaceae: Papilionoideae) and its systematic implications for genistoids and tribe Sophoreae.
[So] Source:PLoS One;12(4):e0173766, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditionally, the tribe Sophoreae sensu lato has been considered a basal but also heterogeneous taxonomic group of the papilionoid legumes. Phylogenetic studies have placed Sophoreae sensu stricto (s.s.) as a member of the core genistoids. The recently suggested new circumscription of this tribe involved the removal of traditional members and the inclusion of Euchresteae and Thermopsideae. Nonetheless, definitions and inter- and intra-taxonomic issues of Sophoreae remain unclear. Within the field of legume systematics, the molecular characteristics of a plastid genome (plastome) have an important role in helping to define taxonomic groups. Here, we examined the plastome of Maackia fauriei, belonging to Sophoreae s.s., to elucidate the molecular characteristics of Sophoreae. Its gene contents are similar to the plastomes of other typical legumes. Putative pseudogene rps16 of Maackia and Lupinus species imply independent functional gene loss from the genistoids. Our overall examination of that loss among legumes suggests that it is common among all major clades of Papilionoideae. The M. fauriei plastome has a novel 24-kb inversion in its large single copy region, as well as previously recognized 50-kb and 36-kb inversions. The 36-kb inversion is shared by the core genistoids. The 24-kb inversion is present in the eight genera belonging to three tribes: Euchresteae, Sophoreae s.s., and Thermopsideae. The phylogenetic distribution of this 24-kb inversion strongly supports the monophyly of members of Sophoreae s.s. with Euchresteae and Thermopsideae. Hence, it can be used as a putative synapomorphic characteristic for the newly circumscribed Sophoreae, including Euchresteae and Thermopsideae. However, plastome conformation suggests a slightly narrower taxonomic group because of heterogeneous results from Bolusanthus and Dicraeopetalum. The phylogenetic analysis, based on plastome sequences from 43 legumes, represents well our understanding of legume systematics while resolving the genistoid clade as a sister group to an Old World clade. It also demonstrates the value that plastomes are powerful marker for systematic studies of basal papilionoid legumes.
[Mh] Termos MeSH primário: Genomas de Plastídeos
Maackia/genética
[Mh] Termos MeSH secundário: Variação Genética
Filogenia
Plastídeos/genética
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173766


  3 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28259967
[Au] Autor:Shen L; Luo Z; Wu J; Qiu L; Luo M; Ke Q; Dong X
[Ad] Endereço:Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
[Ti] Título:Enhanced expression of α2,3-linked sialic acids promotes gastric cancer cell metastasis and correlates with poor prognosis.
[So] Source:Int J Oncol;50(4):1201-1210, 2017 Apr.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Gastric cancer (GC) is a highly metastatic disease and one of the leading causes of cancer death in the world. Aberrant glycosylation is one of many molecular changes that accompany malignant transformation. This study was aimed at identification of glycan profiling changes in GC progression and its potential mechanisms. We employed a microarray with 91 lectins to compare the differential glycans in the three human GC cell lines, SGC-7901, BGC-823 and MGC-803. According to glycan-binding specificities of lectins, all GC cell lines expressed common sugar structures, such as mannose, galactose and fucose. Importantly, we found that the binding of Maackia amurensis lectin-I (MAL-I) to GC cells was proportional to their metastatic capacity. Further analysis revealed that the level of α2,3-linked sialic acids (α2-3Sia), which can be recognized by MAL-I, was significantly overexpressed in MGC-803 cells, while low expression was detected in SGC-7901 cells. In addition, the mRNA and protein expression levels of ß-galactoside α2,3-sialyltransferase IV (ST3Gal-IV), which was related to the synthesis of α2-3Sia, were substantially increased in MGC-803 cells. Knockdown of ST3Gal-IV in MGC-803 cells led to a decreased level of α2-3Sia and decreased ability of invasion and migration. Exogenous expression of ST3Gal-IV in SGC-7901 cells enhanced cell migration, invasion and the content of α2-3Sia. Furthermore, the staining of MAL-I in GC tissues showed that high expression of α2-3Sia was closely correlated with lymph node metastasis, TNM stage and poor overall survival. These findings lead to better understanding of the function of α2-3Sia in the progression and metastasis of GC. This property may be important for developing new therapeutic approaches for GC.
[Mh] Termos MeSH primário: Hexoses/metabolismo
Lectinas/metabolismo
Ácidos Siálicos/biossíntese
Sialiltransferases/metabolismo
Neoplasias Gástricas/metabolismo
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/biossíntese
Linhagem Celular Tumoral
Membrana Celular/metabolismo
Movimento Celular
Feminino
Galactosídeos/metabolismo
Glicosilação
Seres Humanos
Metástase Linfática
Maackia/química
Masculino
Análise em Microsséries
Meia-Idade
Terapia de Alvo Molecular
Estadiamento de Neoplasias
Polissacarídeos/metabolismo
Prognóstico
RNA Mensageiro/metabolismo
Neoplasias Gástricas/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Galactosides); 0 (Hexoses); 0 (Lectins); 0 (Polysaccharides); 0 (RNA, Messenger); 0 (Sialic Acids); 0 (beta-galactoside); EC 2.4.99.- (Sialyltransferases); EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2017.3882


  4 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27720757
[Au] Autor:B S GK; Surolia A
[Ad] Endereço:Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka 560012, India.
[Ti] Título:Comprehensive analysis of α 2-3-linked sialic acid specific Maackia amurensis leukagglutinin reveals differentially occupied N-glycans and C-terminal processing.
[So] Source:Int J Biol Macromol;94(Pt A):114-121, 2017 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Seeds of Maackia amurensis constitutes two sialic acid specific agglutinins known as leukagglutinin and hemagglutinin. Maackia amurensis leukagglutinin (MAL) recognizes α2-3-linked sialic acid present mainly in N-glycans and composed of two disulfide linked monomers. It exhibits potential N-glycosylation sites (four PNGs) which have been assumed to undergo differential occupancy. In this study we have characterized the site specific macro- and microheterogeneity of monomers in detail by analysing N-glycopeptides and peptides through liquid chromatography coupled to ion trap mass spectrometer in MS mode (LC-MS ). We observed the presence of mainly paucimannose N-glycans at Asn , Asn and Asn whereas a high mannose type with varying Man occurs at Asn . Interestingly Asn and Asn exhibited differential occupancy which was evident by the presence of non-glycosylated peptides. This has contributed to the difference in molecular mass of monomers upon SDS-PAGE. Further the presence of disulfide linked peptides confirmed the covalent linkage of monomers which also undergoes uniform C-terminal processing.
[Mh] Termos MeSH primário: Maackia/química
Fito-Hemaglutininas/química
Ácidos Siálicos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Configuração de Carboidratos
Sequência de Carboidratos
Glicosilação
Processamento de Proteína Pós-Traducional
Análise de Sequência de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytohemagglutinins); 0 (Sialic Acids)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170612
[Lr] Data última revisão:
170612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


  5 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27063013
[Au] Autor:Grishchenko OV; Kiselev KV; Tchernoded GK; Fedoreyev SA; Veselova MV; Bulgakov VP; Zhuravlev YN
[Ad] Endereço:Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Stoletija Str., 159, Vladivostok, 690022, Russia. grishchenkoov@gmail.com.
[Ti] Título:RolB gene-induced production of isoflavonoids in transformed Maackia amurensis cells.
[So] Source:Appl Microbiol Biotechnol;100(17):7479-89, 2016 Sep.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Maackia amurensis Rupr. et Maxim is a valuable leguminous tree grown in the Russian Far East, in China, and in Korea. Polyphenols from the heartwood of this species (primarily stilbenes and isoflavonoids) possess strong hepatoprotective activity. Callus culture of M. amurensis produced isoflavonoids and their derivatives. In pharmacological experiments, the callus complex was at least as effective, as the plant complex. To increase the yield of isoflavonoids, calli were transformed with the rolB gene of Agrobacterium rhizogenes. Neomycin phosphotransferase (nptII) gene was used for transgenic cell selection. Three rolB transgenic callus lines with different levels of the rolB gene expression were established. Insertion of the rolB gene caused alterations in callus structure, growth, and isoflavonoid production, and stronger alterations were observed with higher expression levels. MB1, MB2, and MB4 cultures accumulated 1.4, 1.5, and 2.1 % of dry weight (DW) isoflavonoids, respectively. In contrast, the empty vector-transformed MV culture accumulated 1.22 % DW. Isoflavonoid productivity of the obtained MB1, MB2, and MB4 cultures was equal to 117, 112, and 199 mg/L of medium, respectively, comparing to 106 mg/L for the MV culture. High level of expression of the rolB gene in MB4 culture led to a 2-fold increase in the isoflavonoid content and productivity and reliably increased dry biomass accumulation. Lower expression levels of the rolB gene in MB1 and MB2 calli did not significantly enhance biomass accumulation and isoflavonoid content, although the rolB gene activated isoflavonoid biosynthesis during the early growth stages and caused the increased content of several distinct compounds.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Isoflavonas/biossíntese
Maackia/genética
Maackia/metabolismo
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas/metabolismo
beta-Glucosidase/genética
[Mh] Termos MeSH secundário: Agrobacterium/genética
Regulação da Expressão Gênica de Plantas
Isoflavonas/química
Canamicina Quinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Isoflavones); 0 (Plant Proteins); EC 2.7.1.95 (Kanamycin Kinase); EC 3.2.1.- (RolB protein, Agrobacterium rhizogenes); EC 3.2.1.21 (beta-Glucosidase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160412
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7483-y


  6 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26146020
[Au] Autor:Tan M; Wei C; Huang P; Fan Q; Quigley C; Xia M; Fang H; Zhang X; Zhong W; Klassen JS; Jiang X
[Ad] Endereço:1] Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH [2] Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH.
[Ti] Título:Tulane virus recognizes sialic acids as cellular receptors.
[So] Source:Sci Rep;5:11784, 2015 Jul 06.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo-blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell culture model. In this study, we demonstrated that Tulane virus (TV), a cultivable primate calicivirus, also recognizes SAs in addition to the previously known TV-HBGA interactions. Evidence supporting this discovery includes that TV virions bound synthetic sialoglycoconjugates (SGCs) and that treatment of TV permissive LLC-MK2 cells with either neuraminidases or SA-binding lectins inhibited TV infectivity. In addition, we found that Maackia amurensis leukoagglutinin (MAL), a lectin that recognizes the α-2,3 linked SAs, bound LLC-MK2 cells, as well as TV, by which MAL promoted TV infectivity in cell culture. Our findings further highlight TV as a valuable surrogate for huNoVs, particularly in studying virus-host interactions that may involve two host carbohydrate receptors or co-receptors for infection.
[Mh] Termos MeSH primário: Caliciviridae/fisiologia
Receptores de Superfície Celular/metabolismo
Ácidos Siálicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Antígenos de Grupos Sanguíneos/genética
Antígenos de Grupos Sanguíneos/metabolismo
Caliciviridae/isolamento & purificação
Linhagem Celular
Fezes/virologia
Interações Hospedeiro-Patógeno
Seres Humanos
Maackia/metabolismo
Macaca mulatta/virologia
Microscopia de Fluorescência
Neuraminidase/metabolismo
Norovirus/fisiologia
Fito-Hemaglutininas/química
Fito-Hemaglutininas/metabolismo
RNA Viral/análise
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Superfície Celular/química
Ácidos Siálicos/química
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Group Antigens); 0 (Phytohemagglutinins); 0 (RNA, Viral); 0 (Receptors, Cell Surface); 0 (Sialic Acids); 0 (leukoagglutinins, plants); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150707
[St] Status:MEDLINE
[do] DOI:10.1038/srep11784


  7 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26088695
[Au] Autor:Birikaki L; Pradeau S; Armand S; Priem B; Márquez-Domínguez L; Reyes-Leyva J; Santos-López G; Samain E; Driguez H; Fort S
[Ad] Endereço:Univ. Grenoble Alpes, CERMAV, 38000 Grenoble (France), Fax: (+33) 4-76-54-72-03.
[Ti] Título:Chemoenzymatic Syntheses of Sialylated Oligosaccharides Containing C5-Modified Neuraminic Acids for Dual Inhibition of Hemagglutinins and Neuraminidases.
[So] Source:Chemistry;21(30):10903-12, 2015 Jul 20.
[Is] ISSN:1521-3765
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A fast chemoenzymatic synthesis of sialylated oligosaccharides containing C5-modified neuraminic acids is reported. Analogues of GM3 and GM2 ganglioside saccharidic portions where the acetyl group of NeuNAc has been replaced by a phenylacetyl (PhAc) or a propanoyl (Prop) moiety have been efficiently prepared with metabolically engineered E. coli bacteria. GM3 analogues were either obtained by chemoselective modification of biosynthetic N-acetyl-sialyllactoside (GM3 NAc) or by direct bacterial synthesis using C5-modified neuraminic acid precursors. The latter strategy proved to be very versatile as it led to an efficient synthesis of GM2 analogues. These glycomimetics were assessed against hemagglutinins and sialidases. In particular, the GM3 NPhAc displayed a binding affinity for Maackia amurensis agglutinin (MAA) similar to that of GM3 NAc, while being resistant to hydrolysis by Vibrio cholerae (VC) neuraminidase. A preliminary study with influenza viruses also confirmed a selective inhibition of N1 neuraminidase by GM3 NPhAc, suggesting potential developments for the detection of flu viruses and for fighting them.
[Mh] Termos MeSH primário: Hemaglutininas/metabolismo
Engenharia Metabólica
Ácidos Neuramínicos/síntese química
Neuraminidase/antagonistas & inibidores
Oligossacarídeos/síntese química
Ácidos Siálicos/síntese química
Vibrio cholerae/enzimologia
[Mh] Termos MeSH secundário: Aglutininas/metabolismo
Animais
Bovinos
Escherichia coli/genética
Escherichia coli/metabolismo
Hidrólise
Maackia/metabolismo
Ácidos Neuramínicos/química
Ácidos Neuramínicos/metabolismo
Ácidos Neuramínicos/farmacologia
Oligossacarídeos/química
Oligossacarídeos/metabolismo
Oligossacarídeos/farmacologia
Ácidos Siálicos/química
Ácidos Siálicos/metabolismo
Ácidos Siálicos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Agglutinins); 0 (Hemagglutinins); 0 (Neuraminic Acids); 0 (Oligosaccharides); 0 (Sialic Acids); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150716
[Lr] Data última revisão:
150716
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150620
[St] Status:MEDLINE
[do] DOI:10.1002/chem.201500708


  8 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26058603
[Au] Autor:Pshenichnyuk SA; Elkin YN; Kulesh NI; Lazneva EF; Komolov AS
[Ad] Endereço:Institute of Molecule and Crystal Physics, Ufa Research Centre, Russian Academy of Sciences, Prospect Oktyabrya 151, 450075 Ufa, Russia. sapsh@anrb.ru.
[Ti] Título:Low-energy electron interaction with retusin extracted from Maackia amurensis: towards a molecular mechanism of the biological activity of flavonoids.
[So] Source:Phys Chem Chem Phys;17(26):16805-12, 2015 Jul 14.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antioxidant isoflavone retusin efficiently attaches low-energy electrons in vacuo, generating fragment species via dissociative electron attachment (DEA), as has been shown by DEA spectroscopy. According to in silico results obtained by means of density functional theory, retusin is able to attach solvated electrons and could be decomposed under reductive conditions in vivo, for instance, near the mitochondrial electron transport chain, analogous to gas-phase DEA. The most intense decay channels of retusin temporary negative ions were found to be associated with the elimination of H atoms and H2 molecules. Doubly dehydrogenated fragment anions were predicted to possess a quinone structure. It is thought that molecular hydrogen, known for its selective antioxidant properties, can be efficiently generated via electron attachment to retusin in mitochondria and may be responsible for its antioxidant activity. The second abundant species, i.e., quinone bearing an excess negative charge, can serve as an electron carrier and can return the captured electron back to the respiration cycle. The number of OH substituents and their relative positions are crucial for the present molecular mechanism, which can explain the radical scavenging activity of polyphenolic compounds.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Elétrons
Flavonoides/metabolismo
Maackia/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Flavonoides/química
Flavonoides/isolamento & purificação
Maackia/química
Teoria Quântica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150625
[Lr] Data última revisão:
150625
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150611
[St] Status:MEDLINE
[do] DOI:10.1039/c5cp02890f


  9 / 59 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25978938
[Au] Autor:Chhetra Lalli R; Kaur K; Dadsena S; Chakraborti A; Srinivasan R; Ghosh S
[Ad] Endereço:Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh 160012, India. Electronic address: rchhetra@gmail.com.
[Ti] Título:Maackia amurensis agglutinin enhances paclitaxel induced cytotoxicity in cultured non-small cell lung cancer cells.
[So] Source:Biochimie;115:93-107, 2015 Aug.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Maackia amurensis agglutinin (MAA) is gaining recognition as the potential diagnostic agent for cancer. Previous studies from our laboratory have demonstrated that this lectin could interact specifically with the cells and biopsy samples of non-small cell lung cancer (NSCLC) origin but not with normal lung fibroblast cells. Moreover, this lectin was also found to induce apoptosis in NSCLC cells. Further, the biological activity of this lectin was shown to survive gastrointestinal proteolysis and inhibit malignant cell growth and tumorigenesis in mice model of melanoma thereby indicating the therapeutic potential of this lectin. Paclitaxel is one of the widely used traditional chemotherapeutic drugs for treatment of NSCLC but it exerts side-effects on normal healthy cells too. Studies have revealed that lectins have potential to act as an adjuvant chemotherapeutic agent in cancer of different origin. Thus, in the present study, an attempt was made to assess the chemo-adjuvant role of MAA in three types of NSCLC cell lines [adenocarcinoma cell line (A549), squamous cell carcinoma cell line (NCI-H520) and large cell carcinoma cell line (NCI-H460)]. We have observed that the non-cytotoxic concentration of this lectin was able to enhance the cytotoxic activity of Paclitaxel even at low dose by inducing apoptosis through intrinsic/mitochondrial pathway in all the three types of NSCLC cell lines, although the involvement of extrinsic pathway of apoptosis in case of NCI-H460 cell line could not be ruled out. Further, this lectin was also found to augment the chemo-preventive activity of this drug by arresting cells in G2-M phase of the cell cycle. Collectively, our results have suggested that Maackia amurensis agglutinin may have the potential to be used as adjuvant chemotherapeutic agent in case of NSCLC.
[Mh] Termos MeSH primário: Aglutininas/farmacologia
Antineoplásicos/farmacologia
Carcinoma Pulmonar de Células não Pequenas/patologia
Neoplasias Pulmonares/patologia
Maackia/química
Paclitaxel/farmacologia
[Mh] Termos MeSH secundário: Aglutininas/metabolismo
Apoptose/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Sinergismo Farmacológico
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Lectinas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Agglutinins); 0 (Antineoplastic Agents); 0 (Plant Lectins); 0 (jacalin); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150713
[Lr] Data última revisão:
150713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150517
[St] Status:MEDLINE


  10 / 59 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25828081
[Au] Autor:Hushegyi A; Bertok T; Damborsky P; Katrlik J; Tkac J
[Ad] Endereço:Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia. Jan.Tkac@savba.sk.
[Ti] Título:An ultrasensitive impedimetric glycan biosensor with controlled glycan density for detection of lectins and influenza hemagglutinins.
[So] Source:Chem Commun (Camb);51(35):7474-7, 2015 May 01.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An impedimetric glycan biosensor with optimised glycan density was applied for the detection of lectins and influenza hemagglutinins down to attomolar concentrations (aM).
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Glicoproteínas de Hemaglutininação de Vírus da Influenza/análise
Lectinas/análise
Polissacarídeos/análise
[Mh] Termos MeSH secundário: Seres Humanos
Vírus da Influenza A Subtipo H1N1/metabolismo
Vírus da Influenza A Subtipo H5N1/metabolismo
Maackia/metabolismo
Microscopia de Força Atômica
Fito-Hemaglutininas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Lectins); 0 (Phytohemagglutinins); 0 (Polysaccharides); 0 (leukoagglutinins, plants)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150402
[St] Status:MEDLINE
[do] DOI:10.1039/c5cc00922g



página 1 de 6 ir para página                
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde