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Pesquisa : B01.650.940.800.575.912.250.401.592 [Categoria DeCS]
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[PMID]:28910286
[Au] Autor:Wu F; Ma J; Meng Y; Zhang D; Pascal Muvunyi B; Luo K; Di H; Guo W; Wang Y; Feng B; Zhang J
[Ad] Endereço:State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
[Ti] Título:Potential DNA barcodes for Melilotus species based on five single loci and their combinations.
[So] Source:PLoS One;12(9):e0182693, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes. Based on inter-specific divergence, we analysed sequences and confirmed that each candidate barcode was able to identify some of the 18 species. The resolution of a single barcode and its combinations ranged from 33.33% to 88.89%. Analysis of pairwise distances showed that matK+rbcL+trnL-F+trnH-psbA+ITS (MRTPI) had the greatest value and rbcL the least. Barcode gap values and similarity value analyses confirmed these trends. The results indicated that an ITS region, successfully identifying 13 of 18 species, was the most appropriate single barcode and that the combination of all five potential barcodes identified 16 of the 18 species. We conclude that MRTPI is the most effective tool for Melilotus species identification. Taking full advantage of the barcode system, a clear taxonomic relationship can be applied to identify Melilotus species and enhance their practical production.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico/métodos
DNA de Plantas/genética
Melilotus/classificação
[Mh] Termos MeSH secundário: Cloroplastos/genética
DNA de Cloroplastos/genética
DNA Intergênico
Loci Gênicos
Variação Genética
Melilotus/genética
Filogenia
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (DNA, Intergenic); 0 (DNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182693


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[PMID]:28426165
[Au] Autor:Sowa P; Grabek-Lejko D; Wesolowska M; Swacha S; Dzugan M
[Ad] Endereço:Department of Chemistry and Food Toxicology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.
[Ti] Título:Hydrogen peroxide-dependent antibacterial action of Melilotus albus honey.
[So] Source:Lett Appl Microbiol;65(1):82-89, 2017 Jul.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Honey originating from different floral sources exhibits the broad spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as nonperoxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennial = 3 and annual = 5, Melilotus officinalis = 1) were collected directly from beekeepers and analysed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolic contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp. The physicochemical properties of melilot honey were specific for light-coloured unifloral honey samples and were not dependent on its botanical and geographical origin (P > 0·05). All tested honey samples exhibited inhibitory activity (above 90%) against Gram-positive bacteria at the concentration of 12·5-25%. Above 30-50% of antibacterial activity of melilot honey was connected with glucose oxidase enzyme action and was destroyed in the presence of catalase. Hydrogen peroxide-dependent antibacterial activity of honey was inversely correlated with its radical scavenging activity (r = -0·67) and phenolic compounds (r = -0·61). Antibacterial action of melilot honey depends not only on hydrogen peroxide produced by glucose oxidase, but also on other nonperoxide bioactive components of honey. SIGNIFICANCE AND IMPACT OF THE STUDY: Melilot honey is used in traditional medicine as an anticoagulant agent due to the possibility of the presence of the coumarin compounds which are specific for Melilotus plant. Melilotus albus is rarely used to produce honey, and antibacterial properties of this variety of honey had not been studied yet. Nine samples of melilot honey produced in different regions of Poland were analysed according to their antibacterial activity which was correlated with physiochemical parameters and antioxidant activity. It was shown that antibacterial activity of melilot honey is created by hydrogen peroxide and other bioactive compounds.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Escherichia coli/efeitos dos fármacos
Mel/análise
Peróxido de Hidrogênio/química
Klebsiella pneumoniae/efeitos dos fármacos
Salmonella/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Criação de Abelhas
Abelhas/metabolismo
Catalase
Melilotus/metabolismo
Testes de Sensibilidade Microbiana
Fenóis/farmacologia
Plantas/química
Polônia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Phenols); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12749


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[PMID]:27626608
[Au] Autor:Sheikh NA; Desai TR; Tirgar PR
[Ad] Endereço:Department of Pharmacology, Smt. C. V. Gajera Pharmacy Mahila College, Amreli, Gujarat, India.
[Ti] Título:Investigation into Iron Chelating and Antioxidant Potential of Melilotus officinalis in Iron Dextran Induced Iron Overloaded Sprague Dawley Rat Model.
[So] Source:Drug Res (Stuttg);66(12):618-627, 2016 Dec.
[Is] ISSN:2194-9387
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Excess of iron leads to generates free radicals, causes organ damage. (Fabaceae) reported to have various pharmacological activities. It contains flavonoids and phenolic compounds which have iron chelating and antioxidant property. Hence, present study was designed to investigate the beneficial effects of different fractions of for the management of iron overload disease and its complications. Iron overload was induced by 6 IP injections of iron dextran (12.5 mg/100 g) uniformly distributed over the period of 30 days. The different fractions of were given orally and Deferoxamine (DFO) subcutaneously for 30 days. The iron chelating and various biochemical parameters were estimated on 15 and 30 day. The different fractions of demonstrated dose dependant in-vitro iron chelating ability. There were significant (P<0.01) iron chelating potential shows in rats treated with methanolic fraction of methanolic extract (MFME) and methanolic fraction of aqueous extract (MFAE) of as compared to disease control (DC) rats. The rats treated with MFME and MFAE of shows significant (P<0.01) antioxidant and vital organ protective effect as compared to DC rats. Better iron chelation was observed on 30 day and at higher dose (300 mg/kg) as compared to 15 day and at lower dose (150 mg/kg). The present study concludes that MFME and MFAE of have reversible iron chelating and antioxidant potential in rats. The study also proves the possible mechanism of action, as an iron chelator by increasing the excretion of iron in urine and feces.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Dextranos/farmacologia
Quelantes de Ferro/farmacologia
Sobrecarga de Ferro/tratamento farmacológico
Complexo Ferro-Dextran/farmacologia
Melilotus/química
[Mh] Termos MeSH secundário: Animais
Antioxidantes/fisiologia
Desferroxamina/farmacologia
Radicais Livres/metabolismo
Ferro
Masculino
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Dextrans); 0 (Free Radicals); 0 (Iron Chelating Agents); 9004-66-4 (Iron-Dextran Complex); E1UOL152H7 (Iron); J06Y7MXW4D (Deferoxamine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE


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[PMID]:27302610
[Au] Autor:Lam HK; Ross JJ; McAdam EL; McAdam SA
[Ad] Endereço:a School of Biological Sciences, University of Tasmania , Hobart , TAS , Australia.
[Ti] Título:The single evolutionary origin of chlorinated auxin provides a phylogenetically informative trait in the Fabaceae.
[So] Source:Plant Signal Behav;11(7):e1197467, 2016 Jul 02.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chlorinated auxin (4-chloroindole-3-acetic acid, 4-Cl-IAA), a highly potent plant hormone, was once thought to be restricted to species of the tribe Fabeae within the Fabaceae, until we recently detected this hormone in the seeds of Medicago, Melilotus and Trifolium species. The absence of 4-Cl-IAA in the seeds of the cultivated species Cicer aeritinum from the Cicerae tribe, immediately basal to the Fabeae and Trifolieae tribes, suggested a single evolutionary origin of 4-Cl-IAA. Here, we provide a more robust phylogenetic placement of the ability to produce chlorinated auxin by screening key species spanning this evolutionary transition. We report no detectable level of 4-Cl-IAA in Cicer echinospermum (a wild relative of C. aeritinum) and 4 species (Galega officinalis, Parochetus communis, Astragalus propinquus and A. sinicus) from tribes or clades more basal or sister to the Cicerae tribe. We did detect 4-Cl-IAA in the dry seeds of 4 species from the genus Ononis that are either basal to the genera Medicago, Melilotus and Trigonella or basal to, but still within, the Fabeae and Trifolieae (ex. Parochetus) clades. We conclude that the single evolutionary origin of this hormone in seeds can be used as a phylogenetically informative trait within the Fabaceae.
[Mh] Termos MeSH primário: Fabaceae/classificação
Fabaceae/metabolismo
Ácidos Indolacéticos/metabolismo
Filogenia
[Mh] Termos MeSH secundário: Evolução Molecular
Fabaceae/genética
Medicago/classificação
Medicago/metabolismo
Melilotus/classificação
Melilotus/metabolismo
Trifolium/classificação
Trifolium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 2519-61-1 (4-chloroindole-3-acetic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160616
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2016.1197467


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[PMID]:27255310
[Au] Autor:Han Y; Wu X; Gu J; Zhao J; Huang S; Yuan H; Fu J
[Ad] Endereço:Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang, 330032, China. hyl1957@163.com.
[Ti] Título:Effects of organic acids on the photosynthetic and antioxidant properties and accumulations of heavy metals of Melilotus officinalis grown in Cu tailing.
[So] Source:Environ Sci Pollut Res Int;23(18):17901-9, 2016 Sep.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The effect of citric acid (CA), acetic acid (Ac), and ethylene diamine tetraacetic acid (EDTA) on the photosynthetic and antioxidant properties and the accumulation of some heavy metals (HMs) of Melilotus officinalis seedling growing in Cu mine tailings for 25 days were studied. Results showed that the formation of photosynthesizing cells of M. officinalis was inhibited by EDTA at 2 mmol/kg. Photosynthetic pigment contents under EDTA of 2 mmol/kg were reduced by 26, 40, and 19 %, respectively, compared to the control. The proline contents in aboveground and underground parts increased as the level of EDTA was enhanced. CA and Ac enhanced the activities of superoxide dismutase (SOD) and peroxidase (POD) in the aboveground parts and EDTA inhibited the activity of POD in the underground parts. The addition of CA promoted significantly the growth of M. officinalis, while the biomass decreased significantly under 2 mmol/kg EDTA. Cu contents in the aboveground parts treated with 0.5 and 2.0 mmol/kg EDTA reached 175.50 and 265.17 µg/g dry weight, respectively. Ac and EDTA treatments promoted Cd to translocate from root to aboveground parts. The result indicated that M. officinalis was a tolerant species of Cu tailing and can be used to remediate Cu contaminated environment, and rationally utilization of organic acids, especially EDTA, in the phytoremediation can improve the growth and metals accumulation of M. officinalis.
[Mh] Termos MeSH primário: Ácido Acético/farmacologia
Antioxidantes/farmacologia
Ácido Cítrico/farmacologia
Ácido Edético/farmacologia
Fotossíntese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Melilotus/efeitos dos fármacos
Melilotus/crescimento & desenvolvimento
Peroxidase/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Plântulas/efeitos dos fármacos
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 2968PHW8QP (Citric Acid); 9G34HU7RV0 (Edetic Acid); EC 1.11.1.7 (Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-6920-x


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[PMID]:26507574
[Au] Autor:Cornara L; Xiao J; Burlando B
[Ad] Endereço:a Dipartimento di Scienze della Terra dell'Ambiente e della Vita , Università degli Studi di Genova , Genova , Italy.
[Ti] Título:Therapeutic Potential of Temperate Forage Legumes: A Review.
[So] Source:Crit Rev Food Sci Nutr;56 Suppl 1:S149-61, 2016 Jul 29.
[Is] ISSN:1549-7852
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. Temperate forage legumes are Fabaceae species that include worldwide-important crops. These plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. We have examined here Medicago sativa (alfalfa), Trifolium pratense and T. repens (clovers), Melilotus albus and M. officinalis (sweet clovers), Lotus corniculatus (birdsfoot trefoil), Onobrychis viciifolia (sainfoin), Lespedeza capitata (roundhead lespedeza), and Galega officinalis (goat's rue). The phytochemical complexes of these species contain secondary metabolites whose pharmacological potentials deserve investigation. Major classes of compounds include alkaloids and amines, cyanogenic glycosides, flavonoids, coumarins, condensed tannins, and saponins. Some of these phytochemicals have been related to antihypercholesterolemia, antidiabetic, antimenopause, anti-inflammatory, antiedema, anthelmintic, and kidney protective effects. Two widely prescribed drugs have been developed starting from temperate forage legumes, namely, the antithrombotic warfarin, inspired from sweet clover's coumarin, and the antidiabetic metformin, a derivative of sainfoin's guanidine. Available evidence suggests that temperate forage legumes are a potentially important resource for the extraction of active principles to be used as nutraceuticals and pharmaceuticals.
[Mh] Termos MeSH primário: Fabaceae/química
Compostos Fitoquímicos/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/análise
Anti-Inflamatórios/farmacologia
Ensaios Clínicos como Assunto
Cumarínicos/análise
Cumarínicos/farmacologia
Modelos Animais de Doenças
Fibrinolíticos/análise
Fibrinolíticos/farmacologia
Flavonoides/análise
Flavonoides/farmacologia
Galega/química
Seres Humanos
Hipoglicemiantes/análise
Hipoglicemiantes/farmacologia
Lespedeza/química
Loteae/química
Medicago/química
Medicago sativa/química
Melilotus/química
Compostos Fitoquímicos/análise
Proantocianidinas/análise
Proantocianidinas/farmacologia
Saponinas/análise
Saponinas/farmacologia
Trifolium/química
Varfarina/análise
Varfarina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Coumarins); 0 (Fibrinolytic Agents); 0 (Flavonoids); 0 (Hypoglycemic Agents); 0 (Phytochemicals); 0 (Proanthocyanidins); 0 (Saponins); 5Q7ZVV76EI (Warfarin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151029
[St] Status:MEDLINE
[do] DOI:10.1080/10408398.2015.1038378


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[PMID]:26324856
[Au] Autor:Popp D; Schrader S; Kleinsteuber S; Harms H; Sträuber H
[Ad] Endereço:Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.
[Ti] Título:Biogas production from coumarin-rich plants--inhibition by coumarin and recovery by adaptation of the bacterial community.
[So] Source:FEMS Microbiol Ecol;91(9):fiv103, 2015 Sep.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants like sweet clover (Melilotus spp.) are not suitable as fodder for cattle because of harmful effects of the plant secondary metabolite coumarin. As an alternative usage, the applicability of coumarin-rich plants as substrates for biogas production was investigated. When coumarin was added to continuous fermentation processes codigesting grass silage and cow manure, it caused a strong inhibition noticeable as decrease of biogas production by 19% and increase of metabolite concentrations to an organic acids/alkalinity ratio higher than 0.3(gorganic acids) gCaCO3 (-1). Microbial communities of methanogenic archaea were dominated by the genera Methanosarcina (77%) and Methanoculleus (11%). This community composition was not influenced by coumarin addition. The bacterial community analysis unraveled a divergence caused by coumarin addition correlating with the anaerobic degradation of coumarin and the recovery of the biogas process. As a consequence, biogas production resumed similar to the coumarin-free control with a biogas yield of 0.34 LN g(volatile solids) (-1) and at initial metabolite concentrations (∼ 0.2 g(organic acids) gCaCO3 (-1)). Coumarin acts as inhibitor and as substrate during anaerobic digestion. Hence, coumarin-rich plants might be suitable for biogas production, but should only be used after adaptation of the microbial community to coumarin.
[Mh] Termos MeSH primário: Biocombustíveis/microbiologia
Reatores Biológicos/microbiologia
Cumarínicos/metabolismo
Silagem/microbiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Anaerobiose/fisiologia
Euryarchaeota/classificação
Euryarchaeota/crescimento & desenvolvimento
Euryarchaeota/metabolismo
Fermentação/fisiologia
Esterco/microbiologia
Melilotus/metabolismo
Methanomicrobiaceae/classificação
Methanomicrobiaceae/crescimento & desenvolvimento
Methanosarcina/classificação
Methanosarcina/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biofuels); 0 (Coumarins); 0 (Manure); A4VZ22K1WT (coumarin)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150915
[Lr] Data última revisão:
150915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150902
[St] Status:MEDLINE


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[PMID]:26314034
[Au] Autor:Miwa K; Meinke LJ
[Ad] Endereço:Department of Entomology, University of Nebraska-Lincoln, NE 68583.
[Ti] Título:Diel Patterns of Colaspis brunnea and Colaspis crinicornis (Coleoptera: Chrysomelidae) in Southeastern Nebraska.
[So] Source:Environ Entomol;44(6):1553-61, 2015 Dec.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A field study was conducted to increase our understanding of diel activity patterns of Colaspis brunnea (F.) and Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) in key crop habitats. Within 24-h periods, C. brunnea was sampled in clover fields (primarily red clover, Trifolium pretense (L.), with some sweet clover, Melilotus officinalis (L.) Pallas, and downy brome, Bromus tectorum (L.)) and soybean, Glycine max (L.) Merrill, fields, using a sweep-net, while whole-plant-count sampling was used to monitor C. crinicornis densities in field corn, Zea mays (L.). Sweep-net captures of C. brunnea were significantly greater at night than during the day, suggesting possible vertical movement within the canopy during a 24-h period. Colaspis crinicornis densities on corn plants were fairly constant throughout a 24-h period, but beetle activity (e.g., walking, mating) was significantly greater at night than during the day. Results suggest that both Colaspis species may be exhibiting similar increases in activity at night that facilitates movement from more protected to more exposed areas within a habitat. It is unclear what mechanisms drive this diel pattern, but vegetation architecture and associated interactions with environmental conditions may play a role. Sweep-netting in clover or soybean fields and use of whole-plant-counts in cornfields were effective sampling methods for Colaspis adults. However, because activity and behaviors of Colaspis beetles were influenced by time of day in this study, use of a consistent sampling time within a diel period would be recommended for future population studies or integrated pest management decision-making.
[Mh] Termos MeSH primário: Comportamento Animal/fisiologia
Ritmo Circadiano
Coleópteros/fisiologia
[Mh] Termos MeSH secundário: Animais
Bromus
Locomoção
Melilotus
Nebraska
Densidade Demográfica
Feijão de Soja
Especificidade da Espécie
Trifolium
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150828
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvv132


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[PMID]:26167689
[Au] Autor:Di H; Duan Z; Luo K; Zhang D; Wu F; Zhang J; Liu W; Wang Y
[Ad] Endereço:State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou City, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou City, China.
[Ti] Título:Interspecific Phylogenic Relationships within Genus Melilotus Based on Nuclear and Chloroplast DNA.
[So] Source:PLoS One;10(7):e0132596, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Melilotus comprises 19 species, while the phylogenetic relationships between species remain unclear. In the present work, three chloroplast genes, rbcL, matK, trnL-F, and one nuclear region, ITS (internal transcribed spacer) belonging to 48 populations of 18 species of Melilotus were sequenced and phylogenetic trees were constructed to study their interspecific relationships. Based on the phylogenetic tree generated in this study using rbcL analysis, the Melilotus genus is clearly monophyletic in the legume family. Both Bayesian and maximum-parsimony approaches were used to analyze the data. The nrDNA ITS provided more informative characteristics (9.8%) than cpDNA (3.0%). Melilotus contains two closely related groups, clade I and clade II. M. spicatus, M. indicus and M. segetalis have a close relationship. M. infestus, M. siculus and M. sulcatus are closely related. The comparing between molecular phylogeny and flower color classification in Melilotus showed that the flower color is not much informative for phylogenetics of this genus.
[Mh] Termos MeSH primário: Núcleo Celular/genética
DNA de Cloroplastos/genética
Genes de Plantas
Melilotus/genética
Filogenia
[Mh] Termos MeSH secundário: Melilotus/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0132596


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[PMID]:26071209
[Au] Autor:Spellman KV; Schneller LC; Mulder CP; Carlson ML
[Ad] Endereço:Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK, 99775, USA. katie.spellman@alaska.edu.
[Ti] Título:Effects of non-native Melilotus albus on pollination and reproduction in two boreal shrubs.
[So] Source:Oecologia;179(2):495-507, 2015 Oct.
[Is] ISSN:1432-1939
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The establishment of abundantly flowered, highly rewarding non-native plant species is expected to have strong consequences for native plants through altered pollination services, particularly in boreal forest where the flowering season is short and the pollinator pool is small. In 18 boreal forest sites, we added flowering Melilotus albus to some sites and left some sites as controls in 2 different years to test if the invasive plant influences the pollination and reproductive success of two co-flowering ericaceous species: Vaccinium vitis-idaea and Rhododendron groenlandicum. We found that M. albus increased the pollinator diversity and tended to increase visitation rates to the focal native plant species compared to control sites. Melilotus albus facilitated greater seed production per berry in V. vitis-idaea when we added 120 plants compared to when we added 40 plants or in control sites. In R. groenlandicum, increasing numbers of M. albus inflorescences lowered conspecific pollen loads and percentage of flowers pollinated; however, no differences in fruit set were detected. The number of M. albus inflorescences had greater importance in explaining R. groenlandicum pollination compared to other environmental variables such as weather and number of native flowers, and had greater importance in lower quality black spruce sites than in mixed deciduous and white spruce sites for explaining the percentage of V. vitis-idaea flowers pollinated. Our data suggest that the identity of new pollinators attracted to the invaded sites, degree of shared pollinators between invasive and native species, and variation in resource limitation among sites are likely determining factors in the reproductive responses of boreal native plants in the presence of an invasive.
[Mh] Termos MeSH primário: Ericaceae/fisiologia
Espécies Introduzidas
Melilotus/fisiologia
Polinização
[Mh] Termos MeSH secundário: Meio Ambiente
Flores/fisiologia
Frutas/fisiologia
Pólen/fisiologia
Reprodução
Sementes/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150614
[St] Status:MEDLINE
[do] DOI:10.1007/s00442-015-3364-9



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