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  1 / 2019 MEDLINE  
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[PMID]:28742459
[Au] Autor:Campa A; Trabanco N; Ferreira JJ
[Ad] Endereço:First and third authors: Plant Genetics, Area of Horticultural and Forest Crops, SERIDA, Asturias, Spain; and second author: Division of Plant Production (DiSAA), Università degli Studi di Milano, Via Celoria 2, Milan (Italy).
[Ti] Título:Identification of Clusters that Condition Resistance to Anthracnose in the Common Bean Differential Cultivars AB136 and MDRK.
[So] Source:Phytopathology;107(12):1515-1521, 2017 12.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The correct identification of the anthracnose resistance systems present in the common bean cultivars AB136 and MDRK is important because both are included in the set of 12 differential cultivars proposed for use in classifying the races of the anthracnose causal agent, Colletrotrichum lindemuthianum. In this work, the responses against seven C. lindemuthianum races were analyzed in a recombinant inbred line population derived from the cross AB136 × MDRK. A genetic linkage map of 100 molecular markers distributed across the 11 bean chromosomes was developed in this population to locate the gene or genes conferring resistance against each race, based on linkage analyses and χ tests of independence. The identified anthracnose resistance genes were organized in clusters. Two clusters were found in AB136: one located on linkage group Pv07, which corresponds to the anthracnose resistance cluster Co-5, and the other located at the end of linkage group Pv11, which corresponds to the Co-2 cluster. The presence of resistance genes at the Co-5 cluster in AB136 was validated through an allelism test conducted in the F population TU × AB136. The presence of resistance genes at the Co-2 cluster in AB136 was validated through genetic dissection using the F population ABM3 × MDRK, in which it was directly mapped to a genomic position between 46.01 and 47.77 Mb of chromosome Pv11. In MDRK, two independent clusters were identified: one located on linkage group Pv01, corresponding to the Co-1 cluster, and the second located on LG Pv04, corresponding to the Co-3 cluster. This report enhances the understanding of the race-specific Phaseolus vulgaris-C. lindemuthianum interactions and will be useful in breeding programs.
[Mh] Termos MeSH primário: Colletotrichum/fisiologia
Resistência à Doença/genética
Phaseolus/imunologia
Doenças das Plantas/imunologia
[Mh] Termos MeSH secundário: Cruzamento
Cruzamentos Genéticos
Ligação Genética
Marcadores Genéticos/genética
Phaseolus/microbiologia
Doenças das Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-01-17-0012-R


  2 / 2019 MEDLINE  
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[PMID]:29200506
[Au] Autor:Keller CP
[Ad] Endereço:Department of Biology, Minot State University, 500 University Avenue West, Minot, North Dakota 58707.
[Ti] Título:Leaf expansion in : transient auxin-induced growth increase.
[So] Source:Physiol Plant;130(4):580-589, 2017 Aug.
[Is] ISSN:1399-3054
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Control of leaf expansion by auxin is not well understood. Evidence from short term exogenous applications and from treatment of excised tissues suggests auxin positively influences growth. Manipulations of endogenous leaf auxin content, however, suggests that, long-term, auxin suppresses leaf expansion. This study attempts to clarify the growth effects of auxin on unifoliate (primary) leaves of the common bean ( ) by reexamining the response to auxin treatment of both excised leaf strips and attached leaves. Leaf strips, incubated in culture conditions that promoted steady elongation for up to 48 h, treated with 10 µM NAA responded with an initial surge of elongation growth complete within 10 hours followed by insensitivity. A range of NAA concentrations from 0.1 µM to 300 µM induced increased strip elongation after 24 hours and 48 hours. Increased elongation and epinastic curvature of leaf strips was found specific to active auxins. Expanding attached unifoliates treated once with aqueous auxin α-naphthalene acetic acid (NAA) at 1.0 mM showed both an initial surge in growth lasting 4-6 hours followed by growth inhibition sustained at least as long as 24 hours post treatment. Auxin-induced inhibition of leaf expansion was associated with smaller epidermal cell area. Together the results suggest increasing leaf auxin first increases growth then slows growth through inhibition of cell expansion. Excised leaf strips, retain only the initial increased growth response to auxin and not the subsequent growth inhibition, either as a consequence of wounding or of isolation from the plant.
[Mh] Termos MeSH primário: Ácidos Indolacéticos/farmacologia
Phaseolus/crescimento & desenvolvimento
Reguladores de Crescimento de Planta/farmacologia
Folhas de Planta/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ácidos Naftalenoacéticos/farmacologia
Phaseolus/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Naphthaleneacetic Acids); 0 (Plant Growth Regulators); 33T7G7757C (1-naphthaleneacetic acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1111/j.1399-3054.2007.00916.x


  3 / 2019 MEDLINE  
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[PMID]:28585253
[Au] Autor:Mendoza FA; Cichy KA; Sprague C; Goffnett A; Lu R; Kelly JD
[Ad] Endereço:Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USA.
[Ti] Título:Prediction of canned black bean texture (Phaseolus vulgaris L.) from intact dry seeds using visible/near infrared spectroscopy and hyperspectral imaging data.
[So] Source:J Sci Food Agric;98(1):283-290, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Texture is a major quality parameter for the acceptability of canned whole beans. Prior knowledge of this quality trait before processing would be useful to guide variety development by bean breeders and optimize handling protocols by processors. The objective of this study was to evaluate and compare the predictive power of visible and near infrared reflectance spectroscopy (visible/NIRS, 400-2498 nm) and hyperspectral imaging (HYPERS, 400-1000 nm) techniques for predicting texture of canned black beans from intact dry seeds. Black beans were grown in Michigan (USA) over three field seasons. The samples exhibited phenotypic variability for canned bean texture due to genetic variability and processing practice. Spectral preprocessing methods (i.e. smoothing, first and second derivatives, continuous wavelet transform, and two-band ratios), coupled with a feature selection method, were tested for optimizing the prediction accuracy in both techniques based on partial least squares regression (PLSR) models. RESULTS: Visible/NIRS and HYPERS were effective in predicting texture of canned beans using intact dry seeds, as indicated by their correlation coefficients for prediction (R ) and standard errors of prediction (SEP). Visible/NIRS was superior (R = 0.546-0.923, SEP = 7.5-1.9 kg 100 g ) to HYPERS (R = 0.401-0.883, SEP = 7.6-2.4 kg 100 g ), which is likely due to the wider wavelength range collected in visible/NIRS. However, a significant improvement was reached in both techniques when the two-band ratios preprocessing method was applied to the data, reducing SEP by at least 10.4% and 16.2% for visible/NIRS and HYPERS, respectively. Moreover, results from using the combination of the three-season data sets based on the two-band ratios showed that visible/NIRS (R = 0.886, SEP = 4.0 kg 100 g ) and HYPERS (R = 0.844, SEP = 4.6 kg 100 g ) models were consistently successful in predicting texture over a wide range of measurements. CONCLUSION: Visible/NIRS and HYPERS have great potential for predicting the texture of canned beans; the robustness of the models is impacted by genotypic diversity, planting year and phenotypic variability for canned bean texture used for model building, and hence, robust models can be built based on data sets with high phenotypic diversity in textural properties, and periodically maintained and updated with new data. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Alimentos em Conserva/análise
Phaseolus/química
Espectroscopia de Luz Próxima ao Infravermelho/métodos
[Mh] Termos MeSH secundário: Análise dos Mínimos Quadrados
Controle de Qualidade
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8469


  4 / 2019 MEDLINE  
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[PMID]:29207281
[Au] Autor:Mazur R; Trzcinska-Danielewicz J; Kozlowski P; Kowalewska L; Rumak I; Shiell BJ; Mostowska A; Michalski WP; Garstka M
[Ad] Endereço:Department of Metabolic Regulation, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland. Electronic address: rmazur@biol.uw.edu.pl.
[Ti] Título:Dark-chilling and subsequent photo-activation modulate expression and induce reversible association of chloroplast lipoxygenase with thylakoid membrane in runner bean (Phaseolus coccineus L.).
[So] Source:Plant Physiol Biochem;122:102-112, 2018 Jan.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids. This reaction is the first step in biosynthesis of oxylipins, which play important and diverse roles in stress response. In this study, we identified four LOX genes (PcLOXA, B, C, D) in chilling-sensitive runner bean (Phaseolus coccineus L.) plant and analyzed their expression patterns during long term dark-chilling (4 °C) stress and during day/night (21ºC/4 °C) temperature fluctuations. Three of the four identified LOX genes, namely PcLOXA, PcLOXB and PcLOXD, were induced by wounding stress, while only the PcLOXA was induced by dark-chilling of both detached (wounded) leaves and whole plants. We identified PcLOXA as a chloroplast-targeted LOX protein and investigated its expression during chilling stress in terms of abundance, localization inside chloroplasts and interactions with the thylakoid membranes. The analysis by immunogold electron microscopy has shown that more than 60% of detectable PcLOXA protein was associated with thylakoids, and dark-chilling of leaves resulted in increased amounts of this protein detected within grana margins of thylakoids. This effect was reversible under subsequent photo-activation of chilled leaves. PcLOXA binding to thylakoids is not mediated by the posttranslational modification but rather is based on direct interactions of the protein with membrane lipids; the binding strength increases under dark-chilling conditions.
[Mh] Termos MeSH primário: Temperatura Baixa
Luz
Lipoxigenase/metabolismo
Phaseolus/enzimologia
Proteínas de Plantas/metabolismo
Tilacoides/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 1.13.11.12 (Lipoxygenase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE


  5 / 2019 MEDLINE  
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[PMID]:29190665
[Au] Autor:Delfini J; Moda-Cirino V; Ruas CF; Dos Santos Neto J; Ruas PM; Buratto JS; Ruas EA; Azeredo Gonçalves LS
[Ad] Endereço:Agronomy Department, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid, Londrina, Brazil.
[Ti] Título:Distinctness of Brazilian common bean cultivars with carioca and black grain by means of morphoagronomic and molecular descriptors.
[So] Source:PLoS One;12(11):e0188798, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Common bean (Phaseolus vulgaris L.) is one of the most important legumes for human consumption and is a staple food in the diet of the population of some countries of Latin America, Africa and Asia. The distinction between cultivars is based predominantly on morphological descriptors, which proved inefficient for the differentiation of some cultivars. This study had the objective of describing, distinguishing and evaluating the agronomic potential of 39 common bean cultivars of the carioca and black grain groups registered for cultivation in Brazil, based on 49 morphoagronomic descriptors and microsatellite (simple sequence repeat -SSR) markers. The morphoagronomic traits of each commercial group were characterized in four environments. Thirty-seven SSR markers were used for the molecular description. The morphological data, analyzed by the Shanonon-Weaver index, detected low variability among cultivars for qualitative data. On the other hand, the estimates of variance analysis, relative importance of the traits and hierarchical grouping analysis applied to the quantitative variables showed that the descriptors related to plant morphology were the most important for the carioca group, and those related to seed morphology were determining for the black group. The genetic parameters estimated for SSR markers by hierarchical and Bayesian cluster analysis identified 116 alleles, with 33 and 30 polymorphic loci and 24 and 22 private alleles for the carioca and black groups, respectively. The combined use of morphoagronomic and molecular descriptors improves the distinguishability of cultivars, contributing in a more efficient way to breeding and to the protection of cultivars.
[Mh] Termos MeSH primário: Phaseolus/classificação
[Mh] Termos MeSH secundário: Teorema de Bayes
Análise por Conglomerados
Repetições de Microssatélites/genética
Phaseolus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188798


  6 / 2019 MEDLINE  
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[PMID]:28981522
[Au] Autor:Karmous I; Trevisan R; El Ferjani E; Chaoui A; Sheehan D
[Ad] Endereço:Plant Toxicology and Molecular Biology of Microorganisms, Faculty of Sciences of Bizerta, Zarzouna, Tunisia.
[Ti] Título:Redox biology response in germinating Phaseolus vulgaris seeds exposed to copper: Evidence for differential redox buffering in seedlings and cotyledon.
[So] Source:PLoS One;12(10):e0184396, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In agriculture, heavy metal contamination of soil interferes with processes associated with plant growth, development and productivity. Here, we describe oxidative and redox changes, and deleterious injury within cotyledons and seedlings caused by exposure of germinating (Phaseolus vulgaris L. var. soisson nain hâtif) seeds to copper (Cu). Cu induced a marked delay in seedling growth, and was associated with biochemical disturbances in terms of intracellular oxidative status, redox regulation and energy metabolism. In response to these alterations, modulation of activities of antioxidant proteins (thioredoxin and glutathione reductase, peroxiredoxin) occurred, thus preventing oxidative damage. In addition, oxidative modification of proteins was detected in both cotyledons and seedlings by one- and two-dimensional electrophoresis. These modified proteins may play roles in redox buffering. The changes in activities of redox proteins underline their fundamental roles in controlling redox homeostasis. However, observed differential redox responses in cotyledon and seedling tissues showed a major capacity of the seedlings' redox systems to protect the reduced status of protein thiols, thus suggesting quantitatively greater antioxidant protection of proteins in seedlings compared to cotyledon. To our knowledge, this is the first comprehensive redox biology investigation of the effect of Cu on seed germination.
[Mh] Termos MeSH primário: Cobre/farmacologia
Cotilédone/efeitos dos fármacos
Germinação/efeitos dos fármacos
Phaseolus/efeitos dos fármacos
Plântulas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cotilédone/metabolismo
Germinação/fisiologia
Oxirredução
Phaseolus/metabolismo
Plântulas/metabolismo
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 789U1901C5 (Copper)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184396


  7 / 2019 MEDLINE  
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[PMID]:28895521
[Au] Autor:Cordeiro AB; Ribeiro RA; Helene LCF; Hungria M
[Ad] Endereço:1​Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
[Ti] Título:Rhizobium esperanzae sp. nov., a N2-fixing root symbiont of Phaseolus vulgaris from Mexican soils.
[So] Source:Int J Syst Evol Microbiol;67(10):3937-3945, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Common bean (Phaseolus vulgaris L.) is the most important legume consumed worldwide; its genetic origins lie in the Mesoamerican (main centre) and Andean regions. It is promiscuous in establishing root-nodule symbioses; however, in the centres of origin/domestication, the predominant association is with Rhizobium etli. We have previously identified a new lineage (PEL-3) comprising three strains (CNPSo 661, CNPSo 666 and CNPSo 668 ) isolated from root nodules of common bean in Mexico, and that have now been analysed in more detail. Sequences of the 16S rRNA gene positioned the three strains in a large clade including R. etli. Multilocus sequence analysis (MLSA) with four housekeeping genes (recA, glnII, gyrB and rpoA) positioned the three strains in a clade distinct from all other described species, with 100 % bootstrap support, and nucleotide identity (NI) of the four concatenated genes with the closest species R. etli was 95.0 %. Average nucleotide identity (ANI) values of the whole genome of CNPSo 668 and the closest species, R. etli, was 92.9 %. In the analyses of the symbiotic genes nifH and nodC, the strains comprised a cluster with other rhizobial symbionts of P. vulgaris. Other phenotypic and genotypic traits were determined for the new group and our data support the description of the three CNPSo strains as a novel species, for which the name Rhizobium esperanzae is proposed. The type strain is CNPSo 668 (=UMR 1320 =Z87-8 =LMG 30030 =U 10001 ), isolated from a common-bean nodule in Mexico.
[Mh] Termos MeSH primário: Fixação de Nitrogênio
Phaseolus/microbiologia
Filogenia
Raízes de Plantas/microbiologia
Rhizobium/classificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
México
Tipagem de Sequências Multilocus
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Rhizobium/genética
Rhizobium/isolamento & purificação
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002225


  8 / 2019 MEDLINE  
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[PMID]:28817280
[Au] Autor:Duran NM; Savassa SM; Lima RG; de Almeida E; Linhares FS; van Gestel CAM; Pereira de Carvalho HW
[Ad] Endereço:Laboratory of Nuclear Instrumentation (LIN), Center of Nuclear Energy in Agriculture (CENA), University of São Paulo (USP) , Piracicaba, São Paulo 13416000, Brazil.
[Ti] Título:X-ray Spectroscopy Uncovering the Effects of Cu Based Nanoparticle Concentration and Structure on Phaseolus vulgaris Germination and Seedling Development.
[So] Source:J Agric Food Chem;65(36):7874-7884, 2017 Sep 13.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nanoparticles properties such as solubility, tunable surface charges, and singular reactivity might be explored to improve the performance of fertilizers. Nevertheless, these unique properties may also bring risks to the environment since the fate of nanoparticles is poorly understood. This study investigated the impact of a range of CuO nanoparticles sizes and concentrations on the germination and seedling development of Phaseolus vulgaris L. Nanoparticles did not affect seed germination, but seedling weight gain was promoted by 100 mg Cu L and inhibited by 1 000 mg Cu L of 25 nm CuO and CuSO . Most of the Cu taken up remained in the seed coat with Cu hotspots in the hilum. X-ray absorption spectroscopy unraveled that most of the Cu remained in its pristine form. The higher surface reactivity of the 25 nm CuO nanoparticles might be responsible for its deleterious effects. The present study therefore highlights the importance of the nanoparticle structure for its physiological impacts.
[Mh] Termos MeSH primário: Cobre/farmacologia
Nanopartículas Metálicas/química
Phaseolus/crescimento & desenvolvimento
Sementes/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cobre/química
Germinação/efeitos dos fármacos
Phaseolus/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Plântulas/efeitos dos fármacos
Plântulas/crescimento & desenvolvimento
Sementes/crescimento & desenvolvimento
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
789U1901C5 (Copper)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03014


  9 / 2019 MEDLINE  
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[PMID]:28796793
[Au] Autor:Reed S; Neuman H; Glahn RP; Koren O; Tako E
[Ad] Endereço:USDA-ARS Robert W. Holley Center for Agriculture & Health, Cornell University, Ithaca, NY, United States of America.
[Ti] Título:Characterizing the gut (Gallus gallus) microbiota following the consumption of an iron biofortified Rwandan cream seeded carioca (Phaseolus Vulgaris L.) bean-based diet.
[So] Source:PLoS One;12(8):e0182431, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biofortification is a plant breeding method that introduces increased concentrations of minerals in staple food crops (e.g., legumes, cereal grains), and has shown success in alleviating insufficient Fe intake in various human populations. Unlike other strategies utilized to alleviate Fe deficiency, studies of the gut microbiota in the context of Fe biofortification have not yet been reported, although the consumption of Fe biofortified staple food crops has increased significantly over time. Hence, in this study, we performed a 6-week feeding trial in Gallus gallus (n = 14), aimed to investigate the alterations in the gut microbiome following administration of an Fe biofortified bean-based diet (biofortified, BFe) versus a bean based diet with poorly-bioavailable Fe (standard, SFe). Cream seeded carioca bean based diets were designed in an identical fashion to those used in a recent human clinical trial of Fe biofortified beans in Rwanda. We hypothesized that the different dietary Fe contents in the beans based diets will alter the composition and function of the intestinal microbiome. The primary outcomes were changes in the gut microbiome composition and function analyzed by 16S rRNA gene sequencing. We observed no significant changes in phylogenetic diversity between groups. There were significant differences in the composition of the microbiota between groups, with the BFe group harboring fewer taxa participating in bacterial Fe uptake, increased abundance of bacteria involved in phenolic catabolism, and increased abundance of beneficial butyrate-producing bacteria. Additionally, depletion of key bacterial pathways responsible for bacterial viability and Fe uptake suggest that improvements in Fe bioavailability, in addition to increases in Fe-polyphenol and Fe-phytate complexes due to biofortification, led to decreased concentrations of cecal Fe available for bacterial utilization. Our findings demonstrate that Fe biofortification may improve Fe status without negatively altering the structure and function of the gut microbiota, as is observed with other nutritional methods of Fe supplementation. These results may be used to further improve the efficacy and safety of future biofortification efforts in eradicating global Fe deficiency.
[Mh] Termos MeSH primário: Ração Animal
Galinhas/microbiologia
Alimentos Fortificados
Microbioma Gastrointestinal/genética
Ferro/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Ceco/microbiologia
Dieta
Suplementos Nutricionais
Feminino
Masculino
Metagenoma
Tipagem Molecular
Phaseolus/genética
Phaseolus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
E1UOL152H7 (Iron)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182431


  10 / 2019 MEDLINE  
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[PMID]:28792546
[Au] Autor:Rivera-Vega LJ; Galbraith DA; Grozinger CM; Felton GW
[Ad] Endereço:Department of Entomology, Pennsylvania State University, University Park, PA, United States of America.
[Ti] Título:Host plant driven transcriptome plasticity in the salivary glands of the cabbage looper (Trichoplusia ni).
[So] Source:PLoS One;12(8):e0182636, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Generalist herbivores feed on a wide array of plants and need to adapt to varying host qualities and defenses. One of the first insect derived secretions to come in contact with the plant is the saliva. Insect saliva is potentially involved in both the pre-digestion of the host plant as well as induction/suppression of plant defenses, yet how the salivary glands respond to changes in host plant at the transcriptional level is largely unknown. The objective of this study was to determine how the labial salivary gland transcriptome varies according to the host plant on which the insect is feeding. In order to determine this, cabbage looper (Trichoplusia ni) larvae were reared on cabbage, tomato, and pinto bean artificial diet. Labial glands were dissected from fifth instar larvae and used to extract RNA for RNASeq analysis. Assembly of the resulting sequencing reads resulted in a transcriptome library for T. ni salivary glands consisting of 14,037 expressed genes. Feeding on different host plant diets resulted in substantial remodeling of the gland transcriptomes, with 4,501 transcripts significantly differentially expressed across the three treatment groups. Gene expression profiles were most similar between cabbage and artificial diet, which corresponded to the two diets on which larvae perform best. Expression of several transcripts involved in detoxification processes were differentially expressed, and transcripts involved in the spliceosome pathway were significantly downregulated in tomato-reared larvae. Overall, this study demonstrates that the transcriptomes of the salivary glands of the cabbage looper are strongly responsive to diet. It also provides a foundation for future functional studies that can help us understand the role of saliva of chewing insects in plant-herbivore interactions.
[Mh] Termos MeSH primário: Dieta
Herbivoria/fisiologia
Proteínas de Insetos/metabolismo
Mariposas/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Processamento Alternativo
Animais
Brassica
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/fisiologia
Larva
Lycopersicon esculentum
Mariposas/crescimento & desenvolvimento
Mariposas/imunologia
Phaseolus
Reação em Cadeia da Polimerase em Tempo Real
Saliva/metabolismo
Glândulas Salivares/crescimento & desenvolvimento
Glândulas Salivares/imunologia
Glândulas Salivares/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182636



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