Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.401.734 [Categoria DeCS]
Referências encontradas : 534 [refinar]
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[PMID]:28455256
[Au] Autor:Kim JH; Cho CW; Kim HY; Kim KT; Choi GS; Kim HH; Cho IS; Kwon SJ; Choi SK; Yoon JY; Yang SY; Kang JS; Kim YH
[Ad] Endereço:College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea; Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju, 55365, Republic of Korea; Advanced Radiation Technology Institute, Korea Atomic Energy Research I
[Ti] Título:α-Glucosidase inhibition by prenylated and lavandulyl compounds from Sophora flavescens roots and in silico analysis.
[So] Source:Int J Biol Macromol;102:960-969, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The enzyme α-glucosidase is a good drug target for the treatment of diabetes mellitus. Four minor flavonoids (1-4) from roots of Sophora flavescens showed the inhibitory activity, with IC values ranging from 11.0±0.3 to 50.6±1.3µM, toward α-glucosidase. An enzyme kinetics analysis of them revealed that the compounds 1 and 4 were non-competitive, and compounds 2 and 3 were un-competitive inhibitors. For molecular docking, 3-dimensional structure of α-glucosidase was built by homology modeling. As the result, four compounds 1-4 were confirmed to interact into common binding site of α-glucosidase. In addition, all of the four prenylated and lavandulyl compounds (1-4) were abundant in an ethyl acetate fraction separated from a methanol extract, and the potential inhibitor (3) was extracted best using tetrahydrofuran.
[Mh] Termos MeSH primário: Simulação por Computador
Extratos Vegetais/farmacologia
Raízes de Plantas/química
Prenilação
Sophora/química
Terpenos/química
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/metabolismo
Inibidores de Glicosídeo Hidrolases/farmacologia
Simulação de Acoplamento Molecular
Extratos Vegetais/química
Extratos Vegetais/metabolismo
Conformação Proteica
alfa-Glucosidases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoside Hydrolase Inhibitors); 0 (Plant Extracts); 0 (Terpenes); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28748727
[Au] Autor:Yan S; Pan S; Ji J
[Ad] Endereço:a School of Petrochemical Engineering , Changzhou University , Changzhou , PR China.
[Ti] Título:Silk fabric dyed with extract of sophora flower bud.
[So] Source:Nat Prod Res;32(3):308-315, 2018 Feb.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study analysed the use of sophora flower bud extract for dyeing and the resulting colour character and fastness of dyed silk fabric. The pigment composition on the silk fabric and recycling of this extract were also studied. The results indicated that the dyed silk fabric possessed good washing, rubbing and perspiration fastness, and the pigment composition on the silk fabric was mainly rutin and quercetin. The average recovery rate of the dye was 55.00%. These results demonstrate that the sophora flower bud extract is an effective natural dye.
[Mh] Termos MeSH primário: Corantes/química
Extratos Vegetais/química
Seda/química
Sophora/química
Têxteis
[Mh] Termos MeSH secundário: Precipitação Química
Cromatografia Líquida de Alta Pressão
Corantes/análise
Flores/química
Espectroscopia de Ressonância Magnética
Extratos Vegetais/análise
Quercetina/análise
Rutina/análise
Espectroscopia de Infravermelho com Transformada de Fourier
Têxteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Plant Extracts); 0 (Silk); 5G06TVY3R7 (Rutin); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1359170


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[PMID]:28629108
[Au] Autor:Wang J; Gao M; Liu J; Wang Q; Wang C; Yin Z; Wu C
[Ad] Endereço:Department of Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China.
[Ti] Título:Lactic acid production from Sophora flavescens residues pretreated with sodium hydroxide: Reutilization of the pretreated liquor during fermentation.
[So] Source:Bioresour Technol;241:915-921, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The feasibility of lactic acid production from Sophora flavescens residues (SFRs) pretreated with sodium hydroxide with the reutilization of the pretreated liquor during fermentation was investigated. After sodium hydroxide pretreatment, 67.5% of the lignin was removed, and hydrolysis efficiency increased from 37.3% to 79.2%. The reutilization of pretreated liquor at 50% loading during open fermentation of unwashed SFR increased lactic acid production by 34.1%. The pretreated liquor acted as pH buffer and resulted in stable pH and high cellulase activity during fermentation. Inhibitors in the pretreated liquor did not affect the growth of lactic acid bacteria but severely inhibited the growth of ethanol-producing yeast. Consequently, lactic acid production increased and ethanol production was zero at 50% loading. Water consumption during pretreatment and fermentation with 50% pretreated liquor was 1.341L per 100g SFR, which was 67.6% lower than that during fermentation with washed SFR.
[Mh] Termos MeSH primário: Ácido Láctico
Hidróxido de Sódio
Sophora
[Mh] Termos MeSH secundário: Etanol
Fermentação
Hidrólise
Lignina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
33X04XA5AT (Lactic Acid); 3K9958V90M (Ethanol); 55X04QC32I (Sodium Hydroxide); 9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


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[PMID]:28499934
[Au] Autor:Liu SQ; Zhang ML; Zhang HJ; Liu FZ; Chu RJ; Zhang GX; Zhu L
[Ad] Endereço:Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
[Ti] Título:Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway.
[So] Source:Life Sci;180:36-41, 2017 Jul 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, has been recently found to be beneficial in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, mainly through its anti-inflammatory effect. In the present study, we tested the effect of MAT on ongoing EAE and defined possible mechanisms underlying its effects on myelination and oligodendrocytes. MAIN METHODS: EAE was induced in C57BL/6 mice and MAT treatment was started at disease onset. Clinical scores were monitored daily; spinal cords and the corpus callosum brain region of mice were harvested on day 23 p.i. for inflammatory infiltration and demyelination of the central nervous system. Myelin content and the development of oligodendrocytes and their precursors were determined by immunostaining, and expression of p-Akt, p-mTOR, p-PI3K, and p-P70S6 was determined by Western blot. KEY FINDINGS: MAT effectively suppressed EAE severity and increased the expression of proteolipid protein, a myelin protein that is a marker of CNS myelin. MAT treatment largely increased the number of mature oligodendrocytes, and significantly activated the PI3K/Akt/mTOR signaling pathway, which is required for oligodendrocyte survival and axon myelination. SIGNIFICANCE: These findings demonstrate a beneficial effect of MAT on oligodendrocyte differentiation and myelination during EAE, most likely through activating the PI3K/Akt/mTOR signaling pathway.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Encefalomielite Autoimune Experimental/tratamento farmacológico
Esclerose Múltipla/tratamento farmacológico
Oligodendroglia/efeitos dos fármacos
Quinolizinas/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/isolamento & purificação
Animais
Autoimunidade/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Corpo Caloso/efeitos dos fármacos
Corpo Caloso/patologia
Modelos Animais de Doenças
Encefalomielite Autoimune Experimental/patologia
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Esclerose Múltipla/patologia
Bainha de Mielina/metabolismo
Oligodendroglia/metabolismo
Fosfatidilinositol 3-Quinase/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Quinolizinas/isolamento & purificação
Índice de Gravidade de Doença
Transdução de Sinais/efeitos dos fármacos
Sophora/química
Serina-Treonina Quinases TOR/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Quinolizines); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.1.137 (Phosphatidylinositol 3-Kinase); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); N390W430AC (matrine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170514
[St] Status:MEDLINE


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[PMID]:28407107
[Au] Autor:Cheng S; Qiong; Lu F; Yonezawa T; Yin G; Song Z; Wang Y; Yang J; Zhang W
[Ad] Endereço:From the Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanhai 200438, China (Cheng, Lu, Yonezawa, Yin, Song, Wang, Yang, and Zhang); and The Department of Biology, Tibet University, Lhasa, China (Qiong).
[Ti] Título:Phylogeography of Sophora moorcroftiana Supports Wu's Hypothesis on the Origin of Tibetan Alpine Flora.
[So] Source:J Hered;108(4):405-414, 2017 Jun 01.
[Is] ISSN:1465-7333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wu hypothesized that the Tibetan flora originated mostly from the paleotropical Tertiary flora in the Hengduan Mountains by adapting to the cold and arid environments associated with the strong uplift of the Qinghai-Tibet Plateau (QTP). Here, we combine the phylogeographic history of Sophora moorcroftiana with that of Sophora davidii to explore the speciation of S. moorcroftiana to test this hypothesis. We collected 151 individuals from 17 populations and sequenced 2 chloroplast fragments and the internal transcribed spacer of rDNA. Five chlorotypes and 9 ribotypes were detected but no significant phylogeographic structure was revealed. The integrated results of phylogeographic studies of these 2 species clearly support the progenitor-derivative relationship between them. We infer that the western peripheral population of S. davidii migrated westwards from the Hengduan Mountains to the middle reaches of the Yarlung Zangbo River and differentiated from its ancestor in the process of adaptation to increasingly cold and arid environments with the uplift of the QTP and finally evolved into S. moorcroftiana during the Late Pliocene. In addition, our findings shed light on the idea that natural selection, as imposed by climate differentiation (especially mean diurnal range and precipitation seasonality), directly drove this peripatric speciation event after geographic isolation. The speciation of S. moorcroftiana is a strong case supporting Wu's hypothesis about the origin of Tibet's flora.
[Mh] Termos MeSH primário: DNA Espaçador Ribossômico/genética
Genética Populacional
Filogenia
Sophora/genética
[Mh] Termos MeSH secundário: Clima
DNA de Cloroplastos/genética
DNA de Plantas/genética
Haplótipos
Filogeografia
Análise de Sequência de DNA
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (DNA, Plant); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1093/jhered/esx028


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[PMID]:28368325
[Au] Autor:Zhang J; Wang Y; Zheng W
[Ad] Endereço:Department of Oncology, Henan Academy institute of Traditional Chinese Medicine, Zhengzhou 450000, Henan, China. zhangjunping888@163.com.
[Ti] Título:Development of a Novel Electrochemical Sensor for Determination of Matrine in Sophora flavescens.
[So] Source:Molecules;22(4), 2017 Apr 01.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A simple and sensitive electrochemical sensor fabricated with graphene nanosheets (GNs) and a hydroxyapatite (HA) nanocomposite-modified glassy carbon electrode (GCE) was developed for the determination of matrine (MT). The as-prepared electrode (GNs/HA/GCE) was verified to outperform bare a GCE and GNs-modified electrode with increased oxidation peak currents and the decreased over-potential in the redox process of MT, indicating the great enhancement of electrocatalytic activity toward the oxidation of MT by the composite of GNs and HA. Under the optimized conditions, the oxidation peak currents were related linearly with the concentration of MT, ranging from 2 µM to 3 mM, and the detection limit (S/N = 3) was 1.2 µM. In addition, the proposed electrochemical sensor can be successfully applied in the quantitative determination of MT in extract.
[Mh] Termos MeSH primário: Alcaloides/análise
Técnicas Biossensoriais
Técnicas Eletroquímicas
Quinolizinas/análise
Sophora/química
[Mh] Termos MeSH secundário: Grafite
Nanocompostos/química
Nanocompostos/ultraestrutura
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Quinolizines); 7782-42-5 (Graphite); N390W430AC (matrine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


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[PMID]:28337797
[Au] Autor:Mollazadeh S; Neshati V; Fazly Bazzaz BS; Iranshahi M; Mojarrad M; Naderi-Meshkin H; Kerachian MA
[Ad] Endereço:Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
[Ti] Título:Standardized Sophora pachycarpa Root Extract Enhances Osteogenic Differentiation in Adipose-derived Human Mesenchymal Stem Cells.
[So] Source:Phytother Res;31(5):792-800, 2017 May.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bone defect is an important topic in public health. Novel therapies are based on osteogenic induction by natural antiosteoporotic compounds including plant-derived estrogens. In the current study, the osteogenic potential of Sophora pachycarpa root extract (SPRE) was explored on human adipose-derived mesenchymal stem cells. Herein, adipose-derived mesenchymal stem cells were osteoinducted in the presence of increased concentrations of the extract for 21 days. Then, cell viability was evaluated by MTT assay, and the differentiated cells were stained by Alizarin Red S for calcium deposition and subjected to alkaline phosphatase (ALP) assay for enzymatic activity. To assess the expression of bone-related genes, treated cells were evaluated by real-time polymerase chain reaction. The MTT test demonstrated that SPRE had no toxic effects on the cell viability. Treating the cells with SPRE noticeably promoted ALP activity, mineralization, and mRNA expression of runt-related transcription factor 2 (RUNX2), bone gamma-carboxyglutamate protein (BGLAP), secreted phosphoprotein 1 (SPP1), and collagen type I alpha 1 (COL1A1). Additionally, cells subjected to 0.1 µg/mL SPRE showed the highest osteogenic effects. According to high-performance liquid chromatography fingerprinting of SPRE, the osteoprotective effects of SPRE is probably due to presence of phytochemicals with estrogen-like activity in the extract. Thus, SPRE might be a suitable therapeutic agent for bone defects therapy in the future research. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Células Mesenquimais Estromais/fisiologia
Osteogênese/efeitos dos fármacos
Extratos Vegetais/farmacologia
Raízes de Plantas/química
Sophora/química
[Mh] Termos MeSH secundário: Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/fisiologia
Regulação da Expressão Gênica/fisiologia
Seres Humanos
Osteogênese/fisiologia
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5803


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[PMID]:28335757
[Au] Autor:Kim JM; Lee JH; Lee GS; Noh EM; Song HK; Gu DR; Kim SC; Lee SH; Kwon KB; Lee YR
[Ad] Endereço:Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan, 570-749, Republic of Korea.
[Ti] Título:Sophorae Flos extract inhibits RANKL-induced osteoclast differentiation by suppressing the NF-κB/NFATc1 pathway in mouse bone marrow cells.
[So] Source:BMC Complement Altern Med;17(1):164, 2017 Mar 23.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sophorae Flos (SF) is a composite of flowers and buds of Styphnolobium japonicum (L.) Schott and has been used in traditional Korean and Chinese medicine for the treatment of hemostasis and inflammation. Previous studies reported that SF possesses anti-obesity properties, as well as anti-allergic, anti-proliferative, and anti-inflammatory activities. However, the effect of SF in bone resorption has not been studies. In this study, we examined the potential of SF extract (SFE) to inhibit receptor activator of NF-κB ligand (RANKL) -induced osteoclast differentiation in cultured mouse-derived bone marrow macrophages (BMMs). METHODS: BMMs, that act as osteoclast precursors, were cultured with M-CSF (50 ng/ml) and RANKL (100 ng/ml) for 4 days to generate osteoclasts. Osteoclast differentiation was measured by tartrate-resistant acidic phosphatase (TRAP) staining and the TRAP solution assay. Osteoclast differentiation marker genes were analyzed by the quantitative real-time polymerase chain reaction analysis. RANKLs signaling pathways were confirmed through western blotting. RESULTS: SFE significantly decreased osteoclast differentiation in a dose-dependent manner. SFE inhibited RANKL-induced osteoclastogenesis by suppressing NF-κB activation. By contrast, SFE did not affect phospholipase C gamma 2 or subsequent cAMP response element binding activation. SFE inhibited the RANKL-induced expression of nuclear factor of activated T cells c1 (NFATc1). CONCLUSIONS: SFE attenuated the RANKL-mediated induction of NF-κB through inhibition of IκBα phosphorylation, which contributed to inhibiting of RANKL-induced osteoclast differentiation through downregulation of NFATc1.
[Mh] Termos MeSH primário: Células da Medula Óssea/efeitos dos fármacos
Fatores de Transcrição NFATC/metabolismo
Osteoclastos/efeitos dos fármacos
Osteogênese/efeitos dos fármacos
Extratos Vegetais/farmacologia
Ligante RANK/metabolismo
Sophora/química
[Mh] Termos MeSH secundário: Animais
Células da Medula Óssea/citologia
Células da Medula Óssea/metabolismo
Células Cultivadas
Regulação para Baixo/efeitos dos fármacos
Flores/química
Camundongos
Camundongos Endogâmicos BALB C
NF-kappa B/genética
NF-kappa B/metabolismo
Fatores de Transcrição NFATC/genética
Osteoclastos/citologia
Osteoclastos/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (NFATC Transcription Factors); 0 (Nfatc1 protein, mouse); 0 (Plant Extracts); 0 (RANK Ligand)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170429
[Lr] Data última revisão:
170429
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1550-x


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[PMID]:28236591
[Au] Autor:Yang X; Deng S; Huang M; Wang J; Chen L; Xiong M; Yang J; Zheng S; Ma X; Zhao P; Feng Y
[Ad] Endereço:School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China.
[Ti] Título:Chemical constituents from Sophora tonkinensis and their glucose transporter 4 translocation activities.
[So] Source:Bioorg Med Chem Lett;27(6):1463-1466, 2017 03 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bioassay-guided phytochemical investigation of the EtOAc fraction (ST-EtOAc) from the roots of Sophora tonkinensis resulted in the isolation of a new compound 6aR,11aR-1-hydroxy-4-isoprenyl-maackiain (1), along with 12 known compounds (2-13). The structure of the new compound was established by 1D and 2D NMR, MS data and circular dichroism analysis. Polyprenylated flavonoids 6-9 and 11-13 increased GLUT-4 translocation by the range of 1.35-2.75 folds. Sophoranone (8) exerted the strongest activity with 2.75 folds GLUT-4 translocation enhancement at the concentration of 10µM. This is the first report of the GLUT-4 translocation activity of the plant Sophora tonkinensis.
[Mh] Termos MeSH primário: Transportador de Glucose Tipo 4/metabolismo
Sophora/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucose Transporter Type 4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


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[PMID]:28126209
[Au] Autor:Wang L; Lu J; Sun W; Gu Y; Zhang C; Jin R; Li L; Zhang Z; Tian X
[Ad] Endereço:Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
[Ti] Título:Hepatotoxicity induced by radix Sophorae tonkinensis in mice and increased serum cholinesterase as a potential supplemental biomarker for liver injury.
[So] Source:Exp Toxicol Pathol;69(4):193-202, 2017 Apr 04.
[Is] ISSN:1618-1433
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Radix Sophorae tonkinensis (S. tonkinensis) is used in Chinese folk medicine to treat sore throats, viral hepatitis, and jaundice. However, little is known about the hepatotoxicity induced by it. This study is to investigate hepatotoxicity induced by radix S. tonkinensis and a potential supplemental biomarker for liver injury through acute toxicity, accumulative toxicity, tolerance test, and sub-chronic toxicity. The contents of cytisine (CYT), matrine (MT), and oxymatrine (OMT) in radix S. tonkinensis extracts were determined simultaneously by the method we developed. In the acute toxicity study, mice were scheduled for single oral gavage at doses of 0, 2.4, 3.2, 4.2, 5.6, 7.5g/kg of radix S. tonkinensis extracts respectively. Another three groups of mice received radix S. tonkinensis extracts orally in single doses of 0, 4.3, 5.6g/kg, while the two groups of the hepatic injury model were induced by intraperitoneal injection with 0.1% and 0.2% carbon tetrachloride (CCl ). Mortality rate, analysis of serum biochemistry, and histopathological examination were used to assess the acute toxicity. In the accumulative toxicity study, mice were treated radix S. tonkinensis extracts orally by the method of dose escalation for 20days respectively. Accumulative toxicity was assessed by mortality rate. In the tolerance test, half of the mice of test group in the accumulative toxicity were administered the dose of 4.3g/kg radix S. tonkinensis extracts, and the rest of the mice in the test group were assigned to receive the dose of 5.6g/kg radix S. tonkinensis extracts. In the sub-chronic toxicity study, mice were treated with daily doses of 0, 0.25, 1.0, 2.5g/kg radix S. tonkinensis extracts for 90days. Assessments of body weights, serum biochemical analysis, and histopathological examination were performed. An enzyme-inhibition assay for butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE) of CYT, MT, and OMT was also carried out. The contents of CYT, MT, and OMT in radix S. tonkinensis extracts were 5.63mg/g, 27.63mg/g, and 16.20mg/g respectively. In the acute toxicity study, LD50 of radix S. tonkinensis extracts was 4.3g/kg. No mice were found dead in the accumulative toxicity study. In the acute toxicity and tolerance test, increased ALT, AST, and CHE levels were observed in a dose-response manner, while the severity of histological changes in liver was shown in a dose-dependent mode. In the sub-chronic toxicity, though there was a decline trend of ALT and AST levels found in 0.25g/kg, 1.0g/kg, and 2.5g/kg radix S. tonkinensis extracts as compared to control, which might be related to weight loss, the severity of histopathological changes in the liver and the increased serum CHE level was shown in a dose-response manner. MT, OMT, and CYT showed inhibitory effects on BuChE and AChE in the enzyme-inhibition assay. The results of this study indicate that radix S. tonkinensis should have hepatotoxicity, and increased serum CHE is a potential supplemental biomarker for liver injury.
[Mh] Termos MeSH primário: Doença Hepática Induzida por Substâncias e Drogas/enzimologia
Doença Hepática Induzida por Substâncias e Drogas/patologia
Colinesterases/sangue
Medicamentos de Ervas Chinesas/toxicidade
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Cromatografia Líquida de Alta Pressão
Modelos Animais de Doenças
Camundongos
Camundongos Endogâmicos ICR
Sophora
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Drugs, Chinese Herbal); EC 3.1.1.8 (Cholinesterases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE



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