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[PMID]:29381775
[Au] Autor:Sundaramoorthy J; Park GT; Mukaiyama K; Tsukamoto C; Chang JH; Lee JD; Kim JH; Seo HS; Song JT
[Ad] Endereço:School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
[Ti] Título:Molecular elucidation of a new allelic variation at the Sg-5 gene associated with the absence of group A saponins in wild soybean.
[So] Source:PLoS One;13(1):e0192150, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In soybean, triterpenoid saponin is one of the major secondary metabolites and is further classified into group A and DDMP saponins. Although they have known health benefits for humans and animals, acetylation of group A saponins causes bitterness and gives an astringent taste to soy products. Therefore, several studies are being conducted to eliminate acetylated group A saponins. Previous studies have isolated and characterized the Sg-5 (Glyma.15g243300) gene, which encodes the cytochrome P450 72A69 enzyme and is responsible for soyasapogenol A biosynthesis. In this study, we elucidated the molecular identity of a novel mutant of Glycine soja, 'CWS5095'. Phenotypic analysis using TLC and LC-PDA/MS/MS showed that the mutant 'CWS5095' did not produce any group A saponins. Segregation analysis showed that the absence of group A saponins is controlled by a single recessive allele. The locus was mapped on chromosome 15 (4.3 Mb) between Affx-89193969 and Affx-89134397 where the previously identified Glyma.15g243300 gene is positioned. Sequence analysis of the coding region for the Glyma.15g243300 gene revealed the presence of four SNPs in 'CWS5095' compared to the control lines. One of these four SNPs (G1127A) leads to the amino acid change Arg376Lys in the EXXR motif, which is invariably conserved among the CYP450 superfamily proteins. Co-segregation analysis showed that the missense mutation (Arg376Lys) was tightly linked with the absence of group A saponins in 'CWS5095'. Even though Arg and Lys have similar chemical features, the 3D modelled protein structure indicates that the replacement of Arg with Lys may cause a loss-of-function of the Sg-5 protein by inhibiting the stable binding of a heme cofactor to the CYP72A69 apoenzyme.
[Mh] Termos MeSH primário: Alelos
Genes de Plantas
Saponinas/genética
Feijão de Soja/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Saponins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192150


  2 / 18065 MEDLINE  
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[PMID]:28968940
[Au] Autor:Li Z; Wang P; Menzies NW; Kopittke PM
[Ad] Endereço:The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland, 4072, Australia.
[Ti] Título:Defining appropriate methods for studying toxicities of trace metals in nutrient solutions.
[So] Source:Ecotoxicol Environ Saf;147:872-880, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of inappropriate experimental conditions for examining trace metal phytotoxicity results in data of questionable value. The present study aimed to identify suitable parameters for study of phytotoxic metals in nutrient solutions. First, the literature was reviewed to determine the concentration of six metals (Cd, Cu, Hg, Ni, Pb, and Zn) from solution of contaminated soils. Next, the effects of pH, P, Cl, NO , and four Fe-chelators were investigated by using thermodynamic modelling and by examining changes in root elongation rate of soybean (Glycine max cv. Bunya). The literature review identified that the solution concentrations of metals in soils were low, ranging from (µM) 0.069-11Cd, 0.19-15.8 Cu, 0.000027-0.000079 Hg, 1.0-8.7 Ni, 0.004-0.55 Pb, and 0.4-36.3 Zn. For studies in nutrient solution, pH should generally be low given its effects on solubility and speciation, as should the P concentration due to the formation of insoluble phosphate salts. The concentrations of Cl, NO , and various chelators also influence metal toxicity through alteration of metal speciation. The nutrient solutions used to study metal toxicity should consider environmentally-relevant conditions especially for metal concentrations, with concentrations of other components added at levels that do not substantially alter metal toxicity.
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Hidroponia
Metais Pesados/toxicidade
Feijão de Soja/efeitos dos fármacos
Oligoelementos/toxicidade
[Mh] Termos MeSH secundário: Metais Pesados/análise
Modelos Teóricos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Solubilidade
Soluções
Feijão de Soja/crescimento & desenvolvimento
Termodinâmica
Oligoelementos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Solutions); 0 (Trace Elements)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


  3 / 18065 MEDLINE  
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[PMID]:29339254
[Au] Autor:ElBordiny HS; El-Miligy MM; Kassab SE; Daabees H; Mohamed Ali WA; Abdelhamid Mohamed El-Hawash S
[Ad] Endereço:Pharmaceutical Chemistry Department, Faculty of Pharmacy, Damanhour University, Damanhour, El-Buhaira, 22516, Egypt.
[Ti] Título:Design, synthesis, biological evaluation and docking studies of new 3-(4,5-dihydro-1H-pyrazol/isoxazol-5-yl)-2-phenyl-1H-indole derivatives as potent antioxidants and 15-lipoxygenase inhibitors.
[So] Source:Eur J Med Chem;145:594-605, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:New candidates of 3-(4,5-dihydro-1H-pyrazol/isoxazol-5-yl)-2-phenyl-1H-indole derivatives (4-7) were designed combining the pyrazoline/isoxazoline heterocycles and 2-phenylindole to explore its potential as 15-lipoxygenase (15-LOX) inhibitors. The design of the new derivatives was based on utilizing the antioxidant properties of pyrazoline, 2-phenylindole and the good 15-LOX inhibition properties of indolylpyrazoline. The derivatives were synthesized adopting simple and laboratory friendly reaction conditions to give the target compounds in quantitative yields. The resulting indolylpyrazolines/isoxazolines were evaluated as antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and superoxide dismutase (SOD); indolylpyrazoline (4b) was the most potent antioxidant against SOD assay (IC = 1.78 µM) to be superior to ascorbic by 2 folds. Consistently, (4b) was the most potent inhibitor when tested against Soybean 15-LOX (IC = 3.84 µM) excelling quercetin as standard inhibitor by 1.8 folds. Some of the new derivatives were docked into the active binding site of human 15-LOX (PDB entry 4NRE) emphasizing the most potent derivative (4b) and the least potent one (4c). Docking solutions of compounds (4b), (4c), (5b) and (6c) revealed that (4b) was the only compound that got stabilized into the catalytic pocket of enzyme by π-cation interaction with the catalytic Fe and formation of one hydrogen bond with Ile 676 amino acid. Other derivatives including the least potent one variably got stabilized into the active binding pocket by π-cation interaction with the catalytic Fe but failed to form hydrogen bond with Ile 676. For the future optimization of the generated inhibitors, (i) antioxidant activity against SOD, (ii) the inhibitor stabilization by π-cation interaction with the catalytic Fe and (iii) formation of hydrogen bond with Ile 676 should be regarded.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Araquidonato 15-Lipoxigenase/metabolismo
Desenho de Drogas
Indóis/farmacologia
Inibidores de Lipoxigenase/farmacologia
[Mh] Termos MeSH secundário: Androstenóis/síntese química
Androstenóis/química
Androstenóis/farmacologia
Antioxidantes/síntese química
Antioxidantes/química
Relação Dose-Resposta a Droga
Seres Humanos
Indóis/síntese química
Indóis/química
Inibidores de Lipoxigenase/síntese química
Inibidores de Lipoxigenase/química
Simulação de Acoplamento Molecular
Estrutura Molecular
Pirazóis/síntese química
Pirazóis/química
Pirazóis/farmacologia
Feijão de Soja/enzimologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androstenols); 0 (Antioxidants); 0 (Indoles); 0 (Lipoxygenase Inhibitors); 0 (Pyrazoles); 1XA84ITL1H (azastene); 3QD5KJZ7ZJ (pyrazole); EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


  4 / 18065 MEDLINE  
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[PMID]:29220517
[Au] Autor:Varenhorst AJ; Pritchard SR; O'Neal ME; Hodgson EW; Singh AK
[Ad] Endereço:Plant Science Department, South Dakota State University.
[Ti] Título:Determining the Effectiveness of Three-Gene Pyramids Against Aphis glycines (Hemiptera: Aphididae) Biotypes.
[So] Source:J Econ Entomol;110(6):2428-2435, 2017 12 05.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Since the discovery of Aphis glycines Matsumura (Hemiptera: Aphididae) in the United States, the primary management tactic has been foliar insecticides. Alternative management options such as host plant resistance to A. glycines have been developed and their effectiveness proved. However, the use of host plant resistance was complicated by the discovery of multiple, virulent biotypes of A. glycines in the United States that are capable of overcoming single Rag genes, Rag1 and Rag2, as well as a two-gene pyramid of Rag1+Rag2. However, current models predict that the virulent allele frequency of A. glycines decreases in response to the use of pyramided Rag genes, suggesting that pyramids represent a more sustainable use of these traits. Previous research has demonstrated that virulent biotypes can be effectively managed using a three-gene pyramid of Rag1+Rag2+Rag3. Additional Rag-genes have been discovered (Rag4 and Rag5), but whether the incorporation of these genes into novel three-gene pyramids will improve efficacy is not known. We tested single-gene (Rag1 and Rag2) and pyramid cultivars (Rag1+Rag2, Rag1+Rag2+Rag3, Rag1+Rag2+Rag4) to multiple biotypes in laboratory assays. Our results confirm that the Rag1+Rag2+Rag3 pyramid effectively manages all known A. glycines biotypes when compared with cultivars that are overcome by the associated biotype. Our results indicate that Rag1+Rag2+Rag4 would be an effective management option for biotype-1, biotype-2, and biotype-3 A. glycines, but had a negligible impact on biotype-4.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Herbivoria
Controle Biológico de Vetores/métodos
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Animais
Fenótipo
Melhoramento Vegetal
Feijão de Soja/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox230


  5 / 18065 MEDLINE  
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[PMID]:29478629
[Au] Autor:Jiang L; Feng Z; Dai L; Shang B; Paoletti E
[Ad] Endereço:State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; College of
[Ti] Título:Large variability in ambient ozone sensitivity across 19 ethylenediurea-treated Chinese cultivars of soybean is driven by total ascorbate.
[So] Source:J Environ Sci (China);64:10-22, 2018 Feb.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The sensitivity of Chinese soybean cultivars to ambient ozone (O ) in the field is unknown, although soybean is a major staple food in China. Using ethylenediurea (EDU) as an O protectant, we tested the gas exchange, pigments, antioxidants and biomass of 19 cultivars exposed to 28ppm·hr AOT40 (accumulated O over an hourly concentration threshold of 40ppb) over the growing season at a field site in China. By comparing the average biomass with and without EDU, we estimated the cultivar-specific sensitivity to O and ranked the cultivars from very tolerant (<10% change) to highly sensitive (>45% change), which helps in choosing the best-suited cultivars for local cultivation. Higher lipid peroxidation and activity of the ascorbate peroxidase enzyme were major responses to O damage, which eventually translated into lower biomass production. The constitutional level of total ascorbate in the leaves was the most important parameter explaining O sensitivity among these cultivars. Surprisingly, the role of stomatal conductance was insignificant. These results will guide future breeding efforts towards more O -tolerant cultivars in China, while strategies for implementing control measures of regional O pollution are being implemented. Overall, these results suggest that present ambient O pollution is a serious concern for soybean in China, which highlights the urgent need for policy-making actions to protect this critical staple food.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Antioxidantes/metabolismo
Ozônio/toxicidade
Compostos de Fenilureia/metabolismo
Feijão de Soja/fisiologia
[Mh] Termos MeSH secundário: Ácido Ascórbico
China
Fotossíntese/fisiologia
Folhas de Planta/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Antioxidants); 0 (Phenylurea Compounds); 54924-46-8 (ethylene diurea); 66H7ZZK23N (Ozone); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180227
[St] Status:MEDLINE


  6 / 18065 MEDLINE  
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[PMID]:29381755
[Au] Autor:Caetano JM; Tessarolo G; de Oliveira G; Souza KDSE; Diniz-Filho JAF; Nabout JC
[Ad] Endereço:Campus de Ciências Exatas e Tecnológicas (CCET), Universidade Estadual de Goiás, Anápolis, Goiás, Brazil.
[Ti] Título:Geographical patterns in climate and agricultural technology drive soybean productivity in Brazil.
[So] Source:PLoS One;13(1):e0191273, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The impacts of global climate change have been a worldwide concern for several research areas, including those dealing with resources essential to human well being, such as agriculture, which directly impact economic activities and food security. Here we evaluate the relative effect of climate (as indicated by the Ecological Niche Model-ENM) and agricultural technology on actual soybean productivity in Brazilian municipalities and estimate the future geographic distribution of soybeans using a novel statistical approach allowing the evaluation of partial coefficients in a non-stationary (Geographically Weighted Regression; GWR) model. We found that technology was more important than climate in explaining soybean productivity in Brazil. However, some municipalities are more dependent on environmental suitability (mainly in Southern Brazil). The future environmental suitability for soybean cultivation tends to decrease by up 50% in the central region of Brazil. Meanwhile, southern-most Brazil will have more favourable conditions, with an increase of ca. 25% in environmental suitability. Considering that opening new areas for cultivation can degrade environmental quality, we suggest that, in the face of climate change impacts on soybean cultivation, the Brazilian government and producers must invest in breeding programmes and more general ecosystem-based strategies for adaptation to climate change, including the development of varieties tolerant to climate stress, and strategies to increase productivity and reduce costs (social and environmental).
[Mh] Termos MeSH primário: Feijão de Soja/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Agricultura
Brasil
Mudança Climática
Produtos Agrícolas/crescimento & desenvolvimento
Ecossistema
Meio Ambiente
Abastecimento de Alimentos
Geografia
Seres Humanos
Modelos Biológicos
Modelos Teóricos
Tecnologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191273


  7 / 18065 MEDLINE  
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[PMID]:29333856
[Au] Autor:Xie Y; Zhu X; Li Y; Wang C
[Ad] Endereço:School of Food Science and Technology, Henan University of Technology , Zhengzhou, Henan 450001, People's Republic of China.
[Ti] Título:Analysis of the pH-Dependent Fe(III) Ion Chelating Activity of Anthocyanin Extracted from Black Soybean [Glycine max (L.) Merr.] Coats.
[So] Source:J Agric Food Chem;66(5):1131-1139, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Fe(III) chelating activity of anthocyanin extracted from black soybean coats was investigated at pH 3.0, 5.0, 6.5, 7.0, and 7.4 with fluorescence spectroscopy and microscale thermophoresis (MST). Cyanidin-3-glucoside (C3G) was determined to be 98% of the total anthocyanin by high-performance liquid chromatography. The binding affinity (K ) exhibited significant pH-dependent behavior: K was 9.7167 × 10 , 1.0837 × 10 , 1.4284 × 10 , 5.4550 × 10 , and 3.0269 × 10 M at pH 3.0, 5.0, 6.5, 7.0, and 7.4, respectively (p < 0.05). The MST data showed that ΔG < 0 and ΔH < 0, demonstrating that chelation is spontaneous and exothermic. Because both ΔH and ΔS < 0, the chelation involves hydrogen bonds and/or van der Waals forces for pH 3.0, 5.0, and 6.5. Electrostatic interactions contributed to chelation at pH 7.0 and 7.4 with ΔH < 0 and ΔS > 0. With the formation of chelates, C3G improved the solubility of Fe(III) at pH 6.5, 7.0, and 7.4 to enhance the ferric ion bioavailability, except for aggregation observed at pH 5.0.
[Mh] Termos MeSH primário: Antocianinas/química
Antocianinas/isolamento & purificação
Compostos Férricos/química
Quelantes de Ferro/química
Feijão de Soja/química
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Ligações de Hidrogênio
Concentração de Íons de Hidrogênio
Solubilidade
Espectrometria de Fluorescência
Eletricidade Estática
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Ferric Compounds); 0 (Iron Chelating Agents)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180116
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04719


  8 / 18065 MEDLINE  
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[PMID]:29305261
[Au] Autor:Magni C; Sessa F; Capraro J; Duranti M; Maffioli E; Scarafoni A
[Ad] Endereço:Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Via G. Celoria, 2, 20133, Milan, Italy.
[Ti] Título:Structural and functional insights into the basic globulin 7S of soybean seeds by using trypsin as a molecular probe.
[So] Source:Biochem Biophys Res Commun;496(1):89-94, 2018 01 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The basic 7S globulin (Bg7S) is one of the major globulins of soybean seeds. Despite its dual subunit composition and oligomeric assembly, Bg7S has a compact 3D structure (PDB: 3AUP) which is stabilized by a network of inter- and intra-chain disulphide bridges. Bg7S shares several structural elements with a number of homologous proteins from other seeds, whose function is still uncertain. In this work, Bg7S native conformation was probed by using the proteolytic enzyme trypsin. In spite of the presence of many arginine and lysine residues, the protein resulted extremely recalcitrant to in vitro enzymatic cleavage. Indeed, only two scissile bonds located near the C- and N-termini of the large and small subunits, respectively, were cleaved. The partially cleaved products were stable even at prolonged incubation times. Although the generated small peptide fragments were not covalently bound to the remnant of the main chains, they were held in place, as assessed by denaturing and non-denaturing chromatographic approaches. Moreover, both the already observed pH-dependent association/dissociation behaviour of the protein and its insulin binding capacity were preserved both at neutral and acidic pH values. These results are in line with the growing view that the degradation of seed proteins, either storage and non-storage, may be a controlled process related to specific functionalities.
[Mh] Termos MeSH primário: Globulinas/química
Técnicas de Sonda Molecular
Sementes/química
Proteínas de Soja/química
Feijão de Soja/química
Tripsina/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Modelos Químicos
Modelos Moleculares
Sondas Moleculares/química
Ligação Proteica
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Globulins); 0 (Molecular Probes); 0 (Soybean Proteins); EC 3.4.21.4 (Trypsin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  9 / 18065 MEDLINE  
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[PMID]:29240415
[Au] Autor:Fu QL; Blaney L; Zhou DM
[Ad] Endereço:Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences , Nanjing, Jiangsu 210008, People's Republic of China.
[Ti] Título:Identifying Plant Stress Responses to Roxarsone in Soybean Root Exudates: New Insights from Two-Dimensional Correlation Spectroscopy.
[So] Source:J Agric Food Chem;66(1):53-62, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Roxarsone (ROX) is an organoarsenic feed additive of increasing interest used in the poultry industry. Soybean responses to ROX stress were investigated in root exudates (REs) using two-dimensional correlation spectroscopy (2D-COS) with fluorescence and Fourier transform infrared spectra. Environmentally relevant ROX concentrations caused negligible toxicity to crop growth and photosynthesis activity but blackened soybean roots at high concentrations. 2D-COS analysis revealed that the protein-like fluorophore and C═C and C═O, aliphatic OH, and polysaccharide C-O-H moieties in soybean REs were most sensitive to ROX stress. Heterospectral 2D-COS results suggested that aromatic, amide I, quinone, ketone, and aliphatic functional groups were the foundational components of protein-like and short-wavelength excited humic-like fluorophores in soybean REs. Carboxyl and phenolic moieties were related to the long-wavelength excited humic-like fluorophore. Overall, 2D-COS combined with molecular-based spectral analysis of REs provided an innovative approach to characterize the physiological responses of crops to contaminants at sublethal levels.
[Mh] Termos MeSH primário: Raízes de Plantas/efeitos dos fármacos
Roxarsona/farmacologia
Feijão de Soja/efeitos dos fármacos
Espectrometria de Fluorescência/métodos
Espectroscopia de Infravermelho com Transformada de Fourier/métodos
[Mh] Termos MeSH secundário: Arsênico/metabolismo
Clorofila/metabolismo
Cromatografia Líquida de Alta Pressão
Coccidiostáticos/farmacologia
Poluentes Ambientais/farmacocinética
Poluentes Ambientais/farmacologia
Espectrometria de Massas
Exsudatos de Plantas/química
Exsudatos de Plantas/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Raízes de Plantas/metabolismo
Roxarsona/farmacocinética
Feijão de Soja/química
Feijão de Soja/metabolismo
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coccidiostats); 0 (Environmental Pollutants); 0 (Plant Exudates); 1406-65-1 (Chlorophyll); H5GU9YQL7L (Roxarsone); N712M78A8G (Arsenic)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04706


  10 / 18065 MEDLINE  
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[PMID]:29229386
[Au] Autor:Cui X; Lu L; Wang Y; Yuan X; Chen X
[Ad] Endereço:Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, 210014, PR China.
[Ti] Título:The interaction of soybean reticulon homology domain protein (GmRHP) with Soybean mosaic virus encoded P3 contributes to the viral infection.
[So] Source:Biochem Biophys Res Commun;495(3):2105-2110, 2018 01 15.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Soybean mosaic virus (SMV), a member of the Potyvirus genus, is a prevalent and devastating viral pathogen in soybean-growing regions worldwide. Potyvirus replication occurs in the 6K2-induced viral replication complex at endoplasmic reticulum exit sites. Potyvirus-encoded P3 is also associated with the endoplasmic reticulum and is as an essential component of the viral replication complex, playing a key role in viral replication. This study provides evidence that the soybean (Glycine max) reticulon homology domain protein (designated as GmRHP) interacts with SMV-P3 by using a two-hybrid yeast system to screen a soybean cDNA library. A bimolecular fluorescence complementation assay further confirmed the interaction, which occurred on the cytomembrane, endoplasmic reticulum and cytoskeleton in Nicotiana benthamiana cells. The transient expression of GmRHP can promote the coupling of Turnip mosaic virus replication and cell-to-cell movement in N. benthamiana. The interaction between the membrane protein SMV-P3 and GmRHP may contribute to the potyvirus infection, and GmRHP may be an essential host factor for P3's involvement in potyvirus replication.
[Mh] Termos MeSH primário: Doenças das Plantas/virologia
Potyvirus/fisiologia
Proteínas de Soja/metabolismo
Feijão de Soja/metabolismo
Feijão de Soja/virologia
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Tabaco/metabolismo
Tabaco/virologia
Virulência/fisiologia
Replicação Viral/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Soybean Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE



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