Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.456.500 [Categoria DeCS]
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[PMID]:28460278
[Au] Autor:Kamireddy K; Matam P; P S P; Parvatam G
[Ad] Endereço:Academy of Scientific and Innovative Research (CSIR-CFTRI campus, Mysore), India; Plant Cell Biotechnology Department, CSIR-CFTRI, Mysore-570020, India.
[Ti] Título:Biochemical characterization of a key step involved in 2H4MB production in Decalepis hamiltonii.
[So] Source:J Plant Physiol;214:74-80, 2017 Jul.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Decalepis hamiltonii is widely known for its flavour molecule 2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB), a structural isomer of vanillin. As the biosynthetic pathway of 2H4MB is not known, we hypothesised 2H4MB origins could be from phenylpropanoid pathway (PPP). Accordingly, a study was conducted using PPP inhibitors (viz. piperonylic acid, MDCA and propanil) against in vitro root cultures of D. hamiltonii to find the branch of PPP which catalyses the 2H4MB formation. HPLC analysis was carried out to quantify 2H4MB levels in control and respective inhibitor treated root cultures in vitro. The results obtained revealed that piperonylic acid did not inhibit 2H4MB biosynthesis in the given period, whereas MDCA and propanil had the marked inhibitory effect. The inhibitory effect was evident with 13.2, 33.6 and 37.9% decrease in 2H4MB levels at 50, 100 and 150mM concentration of MDCA respectively in comparison with control roots. Similarly, the inhibitory effect of propanil on 2H4MB biosynthesis was obvious with 23.7, 49.5 and 57.9% decrease in 2H4MB levels at 50, 100 and 150µM concentration of inhibitor respectively when compared with control roots. Propanil showed a greater slow down effect on 2H4MB biosynthesis compared to MDCA. Incorporation of 0.1, 0.5 and 1.0mM ferulic acid as a precursor to in vitro root cultures of D. hamiltonii showed an increase in 2H4MB levels at the rate of 3.1, 107 and 94.1% respectively as quantified by HPLC analysis. However, ferulic acid in conjunction with propanil did not show any increase in 2H4MB levels. This clearly explains that ferulic acid is channelled through the 4-CL (4-coumarate CoA ligase) enzyme, where it would be converted to feruloyl-CoA and could be further converted to 2H4MB in D. hamiltonii.
[Mh] Termos MeSH primário: Apocynaceae/metabolismo
Extratos Vegetais/metabolismo
[Mh] Termos MeSH secundário: Benzaldeídos/metabolismo
Benzoatos/metabolismo
Ácidos Cumáricos/metabolismo
Fenilpropionatos/metabolismo
Raízes de Plantas/metabolismo
Propanil/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzaldehydes); 0 (Benzoates); 0 (Coumaric Acids); 0 (Phenylpropionates); 0 (Plant Extracts); 0 (phenylpropanoid 3,4-(methylenedioxy)cinnamic acid); 709-98-8 (Propanil); AVM951ZWST (ferulic acid); QX3V1NO0KH (piperonylic acid); TA269SD04T (benzaldehyde)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28876059
[Au] Autor:Oghumu S; Varikuti S; Saljoughian N; Terrazas C; Huntsman AC; Parinandi NL; Fuchs JR; Kinghorn AD; Satoskar AR
[Ad] Endereço:College of Public Health, §College of Pharmacy, and ∥Department of Microbiology, The Ohio State University , Columbus, Ohio 43210, United States.
[Ti] Título:Pentalinonsterol, a Constituent of Pentalinon andrieuxii, Possesses Potent Immunomodulatory Activity and Primes T Cell Immune Responses.
[So] Source:J Nat Prod;80(9):2515-2523, 2017 Sep 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The use of natural products as adjuvants has emerged as a promising approach for the development of effective vaccine formulations. Pentalinonsterol (PEN) is a recently isolated compound from the roots of Pentalinon andrieuxii and has been shown to possess antileishmanial activity against Leishmania spp. The objective of this study was to examine the immunomodulatory properties of PEN and evaluate its potential as an adjuvant. Macrophages and bone-marrow-derived dendritic cells (BMDCs) were stimulated with PEN and tested for gene expression, cytokine production, and their ability to activate T cells in vitro. PEN was also evaluated for its ability to generate antigen-specific Th1 and Th2 responses in vivo, following ovalbumin (OVA) immunization using PEN as an adjuvant. The results obtained demonstrate that PEN enhances the expression of NF-κB and AP1 transcription factors, promotes gene expression of Tnfα, Il6, Nos2, and Arg1, and upregulates MHCII, CD80, and CD86 in macrophages. PEN also enhanced IL-12 production in BMDCs and promoted BMDC-mediated production of IFN-γ by T cells. Further, mice immunized with OVA and PEN showed enhanced antigen-specific Th1 and Th2 cytokines in their splenocytes and lymph node cells, as well as increased levels of IgG1 and IgG2 in their sera. Taken together, this study demonstrates that PEN is a potent immunomodulatory compound and potentially can be used as an adjuvant for vaccine development against infectious diseases.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Apocynaceae/química
Citocinas/imunologia
Interleucina-12/imunologia
NF-kappa B/imunologia
Ovalbumina/imunologia
Esteróis/isolamento & purificação
Esteróis/farmacologia
Fator de Necrose Tumoral alfa/farmacologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/química
Animais
Citocinas/biossíntese
Camundongos
Camundongos Endogâmicos BALB C
Estrutura Molecular
NF-kappa B/metabolismo
Ovalbumina/química
Esteróis/química
Linfócitos T
Fator de Necrose Tumoral alfa/química
Fator de Necrose Tumoral alfa/imunologia
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Cytokines); 0 (NF-kappa B); 0 (Pentalinonsterol); 0 (Sterols); 0 (TNF protein, human); 0 (Tumor Necrosis Factor-alpha); 187348-17-0 (Interleukin-12); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00445


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[PMID]:28663081
[Au] Autor:Wang Y; Hang L; Jiao L; Liu H; Li F
[Ad] Endereço:Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, China.
[Ti] Título:Cytotoxic aspidofractinine alkaloids from Kopsia hainanensis.
[So] Source:Fitoterapia;121:53-57, 2017 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Five new aspidofractinine-type alkaloids, kopsiahainins A-E (1-5), were isolated from a 80% EtOH extract of the leaves and stems of Kopsia hainanensis. Structural elucidation of all the compounds were performed by spectral methods such as 1D- and 2D-NMR, IR, UV, and HR-ESI-MS. Alkaloids 3 and 4 exhibited some cytotoxic activity against all of six tested tumor cell lines (BGC-823, HepG2, MCF-7, SGC-7901, SK-MEL-2, and SK-OV-3) with IC values of 7.3-9.5µM and 9.2-10.6µM, respectively.
[Mh] Termos MeSH primário: Apocynaceae/química
Alcaloides de Indol/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
Alcaloides de Indol/isolamento & purificação
Estrutura Molecular
Folhas de Planta/química
Caules de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indole Alkaloids); 0 (aspidofractinine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE


  4 / 879 MEDLINE  
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[PMID]:28662725
[Au] Autor:Alagrafi FS; Alawad AO; Abutaha NM; Nasr FA; Alhazzaa OA; Alharbi SN; Alkhrayef MN; Hammad M; Alhamdan ZA; Alenazi AD; Wadaan MA
[Ad] Endereço:National Center for StemCell Technology (NCSCT), Life Sciences and Environment Research Institute (LSERI), King Abdulaziz City for Science and Technology (KACST), Riyadh, 11442, Saudi Arabia.
[Ti] Título:In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta.
[So] Source:BMC Complement Altern Med;17(1):342, 2017 Jun 29.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rhazya stricta Decne. is a medicinal plant that is widespread in Saudi Arabia and desert areas of the Arabian Peninsula. Its extract contains alkaloids, tannins, and flavonoids that are involved in different biological activities. The study aim was to evaluate the effects of Rhazya stricta plant extracts on the proliferation and differentiation of NTERA-2 (NT2) pluripotent embryonal carcinoma cells. METHODS: Soxhlet extraction was carried out using different solvents to extract stems, leaves and fruit parts of this plant. Cytotoxicity was evaluated by an MTS cell viability assay. The ability of the plant extract to induce cell differentiation was examined phenotypically using an inverted light microscope. The expression of pluripotency markers was investigated by reverse transcriptase polymerase chain reaction (RT-PCR) and immunocytochemistry. Phytochemical screening of chloroform stem extracts was carried out and a chromatographic fingerprint was generated using gas chromatography - mass spectrometry (GC-MS). RESULTS: Chloroform stem extract induced differentiation of NT2 cells at 5 µg/ml, and the differentiated cells exhibited neurite formation. Following induction of differentiation, there was significant down-regulation of the pluripotency marker genes Oct4 and Sox2. In addition, the surface antigen pluripotency marker, TRA-1-60, was strongly down-regulated. Phytochemical analysis of the extract showed the presence of alkaloids and saponins. The chromatogram revealed the presence of fifteen compounds with different retention times. CONCLUSION: Our results demonstrate for the first time that chloroform stem extract of R. stricta can induce neuronal differentiation of stem cells at an early stage and may contain potential therapeutic agent that can be used in neurodegenerative diseases.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apocynaceae/química
Carcinoma Embrionário/fisiopatologia
Diferenciação Celular/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Carcinoma Embrionário/tratamento farmacológico
Carcinoma Embrionário/genética
Carcinoma Embrionário/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Fator 3 de Transcrição de Octâmero/genética
Fator 3 de Transcrição de Octâmero/metabolismo
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Fatores de Transcrição SOXB1/genética
Fatores de Transcrição SOXB1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Octamer Transcription Factor-3); 0 (POU5F1 protein, human); 0 (Plant Extracts); 0 (SOX2 protein, human); 0 (SOXB1 Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1852-7


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[PMID]:28626040
[Au] Autor:Mochizuki K; Furukawa S; Kawakita A
[Ad] Endereço:Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan ko.mochizuki@ecology.kyoto-u.ac.jp.
[Ti] Título:Pollinia transfer on moth legs in (Apocynaceae).
[So] Source:Am J Bot;104(6):953-960, 2017 Jun.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: The genus (Apocynaceae; Asclepiadoideae) is characterized by a set of complex floral characters unique among the asclepiads, but their role in pollination is poorly understood. Here, we report a new mechanism of asclepiad pollination in the wax plant the pollinaria are transferred on the legs of medium or large settling moths. METHODS: Floral visitors and their behavior were observed on Amami-Oshima Island during 2013-2015, and the efficacy of different pollinators was determined by counting the pollinarium loads on different flower visitors. The floral characters were studied to establish the process of pollination in relation to pollinator behavior on the flower. KEY RESULTS: was visited by various settling moths at night, but pollinia attachment was confirmed predominantly on the legs of the large moth (Noctuidae) and less frequently on the legs of the medium-sized moths (Crambidae) and (Geometridae). The moths walked around and searched for nectar on the inflorescence, and the corpusculum became clipped to the arolia (adhesive pads on moth tarsi) as they stepped on the pollinaria between the staminal corona. The downward spherical inflorescence of aggregated flowers with flat, velvety petals and a slippery corona provides restricted footholds for the visitors, which efficiently leads pollinator legs to the pollinaria. CONCLUSIONS: Although the pollination system of is largely unknown, pollination by insect legs may be a major pollination system in this genus because these basic floral characters are shared among many species.
[Mh] Termos MeSH primário: Apocynaceae/fisiologia
Mariposas
Polinização
[Mh] Termos MeSH secundário: Animais
Flores
Néctar de Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Nectar)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1700078


  6 / 879 MEDLINE  
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[PMID]:28603121
[Au] Autor:Ahmed AS; Ahmed Q; Saxena AK; Jamal P
[Ad] Endereço:Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia.
[Ti] Título:Evaluation of in vitro antidiabetic and antioxidant characterizations of Elettaria cardamomum (L.) Maton (Zingiberaceae), Piper cubeba L. f. (Piperaceae), and Plumeria rubra L. (Apocynaceae).
[So] Source:Pak J Pharm Sci;30(1):113-126, 2017 Jan.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:Inhibition of intestinal α-amylase and α-glucosidase is an important strategy to regulate diabetes mellitus (DM). Antioxidants from plants are widely regarded in the prevention of diabetes. Fruits of Elettaria cardamomum (L.) Maton (Zingiberaceae) and Piper cubeba L. f. (Piperaceae) and flowers of Plumeria rubra L. (Apocynaceae) are traditionally used to cure DM in different countries. However, the role of these plants has been grossly under reported and is yet to receive proper scientific evaluation with respect to understand their traditional role in the management of diabetes especially as digestive enzymes inhibitors. Hence, methanol and aqueous extracts of the aforementioned plants were evaluated for their in vitro α-glucosidase and α-amylase inhibition at 1 mg/mL and quantification of their antioxidant properties (DPPH, FRAP tests, total phenolic and total flavonoids contents). In vitro optimization studies for the extracts were also performed to enhance in vitro biological activities. The % inhibition of α-glucosidase by the aqueous extracts of the fruits of E. cardamomum, P. cubeba and flowers of P. rubra were 10.41 (0.03), 95.19 (0.01), and -2.92 (0.03), while the methanol extracts exhibited % inhibition 13.73 (0.02), 92.77 (0.01), and -0.98 (0.01), respectively. The % inhibition of α-amylase by the aqueous extracts were 82.99 (0.01), 64.35 (0.01), and 20.28 (0.02), while the methanol extracts displayed % inhibition 39.93 (0.01), 31.06 (0.02), and 39.40 (0.01), respectively. Aqueous extracts displayed good in vitro antidiabetic and antioxidant activities. Moreover, in vitro optimization experiments helped to increase the α-glucosidase inhibitory activity of E. cardamomum. Our findings further justify the traditional claims of these plants as folk medicines to manage diabetes, however, through digestive enzymes inhibition effect.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Apocynaceae/química
Hipoglicemiantes/farmacologia
Lignanas/farmacologia
Piper/química
Extratos Vegetais/farmacologia
Zingiberaceae/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Compostos de Bifenilo/química
Cloretos/química
Compostos Férricos/química
Flores/química
Frutas/química
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Inibidores de Glicosídeo Hidrolases/farmacologia
Hipoglicemiantes/química
Hipoglicemiantes/isolamento & purificação
Lignanas/química
Lignanas/isolamento & purificação
Metanol/química
Oxirredução
Fitoterapia
Picratos/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Solventes/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Chlorides); 0 (Ferric Compounds); 0 (Glycoside Hydrolase Inhibitors); 0 (Hypoglycemic Agents); 0 (Lignans); 0 (Picrates); 0 (Piper cubeba extract); 0 (Plant Extracts); 0 (Solvents); 059QF0KO0R (Water); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); U38V3ZVV3V (ferric chloride); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


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[PMID]:28598616
[Au] Autor:Kaneta Y; Arai MA; Ishikawa N; Toume K; Koyano T; Kowithayakorn T; Chiba T; Iwama A; Ishibashi M
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
[Ti] Título:Identification of BMI1 Promoter Inhibitors from Beaumontia murtonii and Eugenia operculata.
[So] Source:J Nat Prod;80(6):1853-1859, 2017 Jun 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:B-Cell-specific Moloney murine leukemia virus insertion region 1 (BMI1) is a core component of the polycomb repressive complex 1 (PRC1). Abnormal expression of BMI1 is associated with a number of human malignances and cancer stem cells (CSCs), which cause chemotherapy resistance. Therefore, small molecules that inhibit BMI1 expression are potential candidates for cancer therapy. In this study, a cell-based reporter gene assay was developed that allowed BMI1 promoter activity to be measured in 293T human embryonic kidney cells based on luciferase expression levels. Using this screening assay, the methanol-soluble extracts of Beaumontia murtonii and Eugenia operculata were selected as leads. Bioassay-guided fractionation of the extracts led to the isolation of three known cardenolides (1-3) and one new compound (4) from B. murtonii and two known triterpenoids (5 and 6) and one new compound (7) from E. operculata. These seven compounds inhibited BMI1 promoter activity (IC range 0.093-23.0 µM), and the most active compound, wallichoside (1), was further evaluated. Western blot analysis revealed that wallichoside (1) decreases BMI1 protein levels in HCT116 human colon carcinoma cells, and flow cytometry analysis showed that it significantly reduced levels of the CSC biomarker epithelial cell adhesion molecule. Wallichoside (1) also inhibited sphere formation of Huh7 human hepatocellular carcinoma cells, indicating that it diminished the self-renewal capability of CSCs.
[Mh] Termos MeSH primário: Apocynaceae/química
Eugenia/química
Complexo Repressor Polycomb 1/metabolismo
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Western Blotting
Carcinoma Hepatocelular/tratamento farmacológico
Cardenolídeos/química
Cardenolídeos/isolamento & purificação
Cardenolídeos/farmacologia
Linhagem Celular Tumoral
Células HCT116
Células HEK293
Seres Humanos
Concentração Inibidora 50
Neoplasias Hepáticas/tratamento farmacológico
Camundongos
Estrutura Molecular
Células-Tronco Neoplásicas/efeitos dos fármacos
Folhas de Planta/química
Tailândia
Triterpenos/química
Triterpenos/isolamento & purificação
Triterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (BMI1 protein, human); 0 (Cardenolides); 0 (Triterpenes); EC 2.3.2.27 (Polycomb Repressive Complex 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00138


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[PMID]:28561586
[Au] Autor:Seeka C; Prabpai S; Kongsaeree P; Tewtrakul S; Lhinhatrakool T; Sutthivaiyakit S
[Ad] Endereço:Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University , Hua Mark, Bangkapi, Bangkok 10240, Thailand.
[Ti] Título:Anti-inflammatory 12,20-Epoxypregnane and 11,12-seco-Pregnane Glycosides from the Stems of Hoya kerrii.
[So] Source:J Nat Prod;80(6):1714-1724, 2017 Jun 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Five 12,20-epoxypregnane glycosides (1-3, 5, and 6) and two 11,12-seco-pregnane glycosides (4 and 7) with spirodilactone motifs, as well as spirodilactone cleavage products 8 and 9, were isolated from the stems of Hoya kerrii. The relative configurations of the three related skeletons were supported by ROESY experiments and X-ray crystallographic analyses. The isolates were evaluated for their anti-inflammatory activity based on the inhibition of NO production in RAW264.7 cells, and some showed IC values ranging from 12.6 to 96.5 µM. The most potent compound, 9a, was also examined for its anti-inflammatory mechanism against mRNA expression and was found to down-regulate mRNA expression of iNOS and COX-2 in a dose-dependent manner.
[Mh] Termos MeSH primário: Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Apocynaceae/química
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Pregnanos/isolamento & purificação
Pregnanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Cristalografia por Raios X
Ciclo-Oxigenase 2/efeitos dos fármacos
Ciclo-Oxigenase 2/metabolismo
Relação Dose-Resposta a Droga
Glicosídeos/química
Concentração Inibidora 50
Lipopolissacarídeos/farmacologia
Macrófagos/metabolismo
Camundongos
Conformação Molecular
Estrutura Molecular
Óxido Nítrico/biossíntese
Óxido Nítrico Sintase Tipo II/efeitos dos fármacos
Ressonância Magnética Nuclear Biomolecular
Caules de Planta/química
Pregnanos/química
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Glycosides); 0 (Lipopolysaccharides); 0 (Pregnanes); 0 (pregnane glycoside); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00730


  9 / 879 MEDLINE  
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[PMID]:28520766
[Au] Autor:Hajrah NH; Obaid AY; Atef A; Ramadan AM; Arasappan D; Nelson CA; Edris S; Mutwakil MZ; Alhebshi A; Gadalla NO; Makki RM; Al-Kordy MA; El-Domyati FM; Sabir JSM; Khiyami MA; Hall N; Bahieldin A; Jansen RK
[Ad] Endereço:Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia.
[Ti] Título:Transcriptomic analysis of salt stress responsive genes in Rhazya stricta.
[So] Source:PLoS One;12(5):e0177589, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rhazya stricta is an evergreen shrub that is widely distributed across Western and South Asia, and like many other members of the Apocynaceae produces monoterpene indole alkaloids that have anti-cancer properties. This species is adapted to very harsh desert conditions making it an excellent system for studying tolerance to high temperatures and salinity. RNA-Seq analysis was performed on R. stricta exposed to severe salt stress (500 mM NaCl) across four time intervals (0, 2, 12 and 24 h) to examine mechanisms of salt tolerance. A large number of transcripts including genes encoding tetrapyrroles and pentatricopeptide repeat (PPR) proteins were regulated only after 12 h of stress of seedlings grown in controlled greenhouse conditions. Mechanisms of salt tolerance in R. stricta may involve the upregulation of genes encoding chaperone protein Dnaj6, UDP-glucosyl transferase 85a2, protein transparent testa 12 and respiratory burst oxidase homolog protein b. Many of the highly-expressed genes act on protecting protein folding during salt stress and the production of flavonoids, key secondary metabolites in stress tolerance. Other regulated genes encode enzymes in the porphyrin and chlorophyll metabolic pathway with important roles during plant growth, photosynthesis, hormone signaling and abiotic responses. Heme biosynthesis in R. stricta leaves might add to the level of salt stress tolerance by maintaining appropriate levels of photosynthesis and normal plant growth as well as by the participation in reactive oxygen species (ROS) production under stress. We speculate that the high expression levels of PPR genes may be dependent on expression levels of their targeted editing genes. Although the results of PPR gene family indicated regulation of a large number of transcripts under salt stress, PPR actions were independent of the salt stress because their RNA editing patterns were unchanged.
[Mh] Termos MeSH primário: Apocynaceae/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Tolerância a Sal/genética
Estresse Fisiológico/genética
Transcriptoma
[Mh] Termos MeSH secundário: Apocynaceae/metabolismo
Análise por Conglomerados
Biologia Computacional/métodos
Ontologia Genética
Sequenciamento de Nucleotídeos em Larga Escala
Família Multigênica
Folhas de Planta
Salinidade
Tetrapirróis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tetrapyrroles)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177589


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[PMID]:28480400
[Au] Autor:Bamigboye J T; Josephine Y O; Olujide O O; A OI; Shakir A M A; Mark R J E; Raymond C F J
[Ad] Endereço:Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University. Ile Ife. Nigeria.
[Ti] Título:ISOLATION OF NOVEL PARA-PENTYL PHENYL BENZOATE FROM . (HOOK.F.) SKEELS (PERIPLOCACEAE), ITS STRUCTURE, SYNTHESIS AND NEUROPHARMACOLOGICAL EVALUATION.
[So] Source:Afr J Tradit Complement Altern Med;14(1):219-230, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: L. (Hook. F.) Skeels (Periplocaceae) is a medicinal plant used locally in managing pain, fever, loss of appetite and as aphrodiasc in the South-Western states of Nigeria. However, the fruit is consumed habitually in the South-Eastern states of Nigeria, leading to speculation that it may possess some central nervous system effect but which has not been scientifically investigated, hence this study. METHODOLOGY: Fresh fruits of were collected and identified by a taxonomist. They were chopped into small pieces and extracted with absolute ethanol. The crude extract was subjected to various chromatographic techniques to isolate a novel compound whose structure was elucidated from the analysis of the crystal data and by extensive use of spectroscopy. The structure was confirmed by synthesis. The compound was subjected to anxiolytic and sedative activity assay. Computational analysis of the receptor binding event of isolated compound at the gamma amino butyric acid A receptor was also evaluated. RESULTS: The structure of the compound was elucidated as pentyl phenyl benzoate. The neuropharmacological evaluation of the compound indicated significant (p<0.05) depression of the central nervous system. The binding characteristics of the compound to gamma amino butyric acid A receptors appears to be more favorable than those obtained for gamma amino butyric acid, chlorpromazine, benzamidine, and is comparable with the affinity obtained for pentobarbitone and diazepam. CONCLUSION: These present data provide evidence for the role of pentyl phenyl benzoate in the habitual consumption of the fruit as well as its central nervous system activities.
[Mh] Termos MeSH primário: Apocynaceae/química
Benzoatos/química
Benzoatos/isolamento & purificação
Fármacos do Sistema Nervoso Central/química
Fármacos do Sistema Nervoso Central/isolamento & purificação
Extratos Vegetais/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Benzoatos/síntese química
Benzoatos/farmacologia
Sistema Nervoso Central/efeitos dos fármacos
Sistema Nervoso Central/fisiologia
Fármacos do Sistema Nervoso Central/síntese química
Fármacos do Sistema Nervoso Central/farmacologia
Camundongos
Neurofarmacologia
Nigéria
Extratos Vegetais/síntese química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoates); 0 (Central Nervous System Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i1.24



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