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Pesquisa : B01.650.940.800.575.912.250.456.500.043 [Categoria DeCS]
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[PMID]:28393571
[Au] Autor:Yan TL; Han DX; Hu J; Huang XY; Wang HK
[Ad] Endereço:a Department of Basic Medicine , Qujing Medical College , Qujing 655000 , China.
[Ti] Título:Monoterpenoid indole alkaloids from Alstonia mairei and their cytotoxicity.
[So] Source:J Asian Nat Prod Res;19(6):550-556, 2017 Jun.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation on the 70% ethanol extract of the leaves of Alstonia mairei resulted in the isolation of three new monoterpenoid indole alkaloids, alstomairines A-C (1-3), along with one known compound, alpneumine A (4). Structural elucidation of all the compounds was accomplished by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic activities against four osteosarcoma cell lines. Consequently, alkaloids 2 and 3 exhibited cytotoxic activities for all tested tumor cell lines with IC values from 9.2 to 13.0 µM.
[Mh] Termos MeSH primário: Alstonia/química
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Alcaloides de Triptamina e Secologanina/isolamento & purificação
Alcaloides de Triptamina e Secologanina/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Concentração Inibidora 50
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Folhas de Planta/química
Alcaloides de Triptamina e Secologanina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Secologanin Tryptamine Alkaloids); 0 (alstomairine B); 0 (alstomairine C)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2017.1313242


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[PMID]:28314480
[Au] Autor:Zhao YL; Cao J; Shang JH; Liu YP; Khan A; Wang HS; Qian Y; Liu L; Ye M; Luo XD
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
[Ti] Título:Airways antiallergic effect and pharmacokinetics of alkaloids from Alstonia scholaris.
[So] Source:Phytomedicine;27:63-72, 2017 Apr 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Alstonia scholaris (L.) R. Br. (Apocynaceae), an important herbal medicine, has been widely used to treat respiratory tract diseases, such as cough, asthma, phlegm, and chronic obstructive pulmonary disease. PURPOSE: To evaluate pharmacological effect of alkaloids from A. scholaris on ovalbumin induced airways allergic inflammatory model, and explore whether the dosing frequency is related to pharmacokinetics. STUDY DESIGN: After oral administration of total alkaloids, the pharmacokinetic study of it was investigated. In addition, anti-allergic studies were carried out on ovalbumin-sensitized airways allergic inflammatory model of mice. METHODS: The pharmacokinetics of total alkaloids (TA) was investigated in SD rat plasma by a fully-validated LC-MS/MS method. Then, an ovalbumin (OVA)-sensitized airways allergic inflammatory model was established, in which mice were intra-gastrically administrated by 3 times a day (8.3 and 16.7mg/kg) based on the pharmacokinetic behavior of TA) and single (25, 50mg/kg) treatment regimen. Dexamethasone was used as a positive control for corticosteroid drugs. Cellular infiltration was assessed in the broncho-alveolar lavage fluid (BALF). Expressions of interleukin-4 (IL-4) and interleukin-10 (IL-10) in the BALF were determined, levels of immunoglobulin E (IgE) and eotaxin in serum were measured, and superoxide dismutase (SOD) activities as well as malondialdehyde (MDA) content in the serum and BALF were examined. Finally, histopathological examination in the lung was assessed by H. E. staining. RESULTS: The time course of plasma concentration of 4 bioactive indole alkaloids fitted an open two-compartment model after oral administration of total alkaloids at doses of 10, 25, and 50mg/kg. The area under the curve and the maximum concentration values of four major alkaloids increased dose-dependently, and half-life suggested a short-lasting pharmacological effect. Then, an ovalbumin-provoked airways allergic inflammatory model indicated that the pharmacological effect of administration of total alkaloids 3 times a day was a little better than that of single dose daily. The percentage of eosinophils in BALF was reduced obviously and the pathological damage of lung was also attenuated. There was also a significant reduction in IL-4 and promotion in IL-10 in the BALF. Serum IgE and eotaxin expression also significantly decreased in treated animals. Furthermore, the activity of SOD elevated remarkably and lipid peroxidation product (MDA) decreased in the administrated mice. CONCLUSION: The pharmacological effects administrated for 3 times a day had precedence over single dose daily, which was related to the prolonged retention time and the maintained plasma concentration. Moreover, scholaricine and vallesamine might be responsible for the treatment of allergic asthma, mainly in total alkaloids.
[Mh] Termos MeSH primário: Alcaloides/farmacocinética
Alstonia/química
Antialérgicos/farmacologia
Eosinófilos/efeitos dos fármacos
Hipersensibilidade/tratamento farmacológico
Hipersensibilidade/etiologia
Ovalbumina/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Medicamentos de Ervas Chinesas/farmacocinética
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Fitoterapia
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Allergic Agents); 0 (Drugs, Chinese Herbal); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170319
[St] Status:MEDLINE


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[PMID]:28103857
[Au] Autor:Singh H; Arora R; Arora S; Singh B
[Ad] Endereço:Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, 143005, India.
[Ti] Título:Ameliorative potential of Alstonia scholaris (Linn.) R. Br. against chronic constriction injury-induced neuropathic pain in rats.
[So] Source:BMC Complement Altern Med;17(1):63, 2017 Jan 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Alstonia scholaris commonly known as 'Saptaparni' is an Indian traditional medicinal plant used in Ayurveda. It is commonly used to treat various disorders like asthma, bronchitis, diarrhea, dysentery and malaria. In folklore medicine the milky juice of the plant is applied on wounds and ulcers to treat pain, ear ache and also in rheumatic pains. AIM: The present study was designed to investigate the potential of A. scholaris R. Br. in chronic constriction injury of sciatic nerve (CCI) induced neuropathic pain in rats. METHODS: Peripheral neuropathy was induced by chronic constriction injury of sciatic nerve. The behavioral parameters like mechanical and thermal hyperalgesia and cold allodynia were assessed on the 14 day. Tissue parameters like total protein, thiobarbituric acid reactive substances, reduced glutathione, myeloperoxidase, total calcium and TNF-α were assessed to check biochemical changes. Chloroform and methanol extract of A. scholaris leaves (100 and 200 mg/kg) and pregabalin (10 mg/kg, as positive control) were administered orally for 14 consecutive days starting from the day of surgery. RESULTS: CCI resulted in significant development of mechanical hyperalgesia, heat hyperalgesia and cold allodynia along with alteration in the biochemical changes. Administration of methanol extract at 200 mg/kg significantly attenuated the CCI induced change in nociceptive threshold and biochemical changes which was comparable to that of pregabalin. The high-performance liquid chromatography (HPLC) of the bioactive methanol extract revealed the presence of different types of flavonoids such as gallic acid, catechin, epicatechin, ellagic acid and kaempferol, in which kaempferol was observed to be in higher concentration. CONCLUSION: Methanol extract (200 mg/kg) of A. scholaris showed the ameliorative effect in CCI induced neuropathic pain which may be due to the presence of kaempferol and attributed to its anti-oxidative and anti-inflammatory properties.
[Mh] Termos MeSH primário: Alstonia/química
Neuralgia/tratamento farmacológico
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Clorofórmio
Dor Crônica/tratamento farmacológico
Constrição
Hiperalgesia/tratamento farmacológico
Medicina Ayurvédica
Metanol
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 7V31YC746X (Chloroform); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1577-7


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[PMID]:28040532
[Au] Autor:Li CJ; Chen S; Sun C; Zhang L; Shi X; Wu SJ
[Ad] Endereço:Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.
[Ti] Título:Cytotoxic monoterpenoid indole alkaloids from Alstonia yunnanensis Diels.
[So] Source:Fitoterapia;117:79-83, 2017 Mar.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The ethanol extract of the aerial parts of Alstonia yunnanensis Diels afforded five new monoterpenoid indole alkaloids, alstiyunnanenines A-E (1-5), along with one known compound, alstoniascholarine I (6). The structures of the isolated compounds were established based on 1D and 2D ( H- H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for cytotoxic potential using eight tumor cell lines. As a result, alkaloids 4-6 exhibited cytotoxicities against all tested tumor cell lines, especially against osteosarcoma cell lines (SOSP-9607, MG-63, Saos-2, M663) with IC values<6µM.
[Mh] Termos MeSH primário: Alstonia/química
Antineoplásicos Fitogênicos/química
Alcaloides de Triptamina e Secologanina/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Seres Humanos
Estrutura Molecular
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Alcaloides de Triptamina e Secologanina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Secologanin Tryptamine Alkaloids)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:27974282
[Au] Autor:Akinnawo OO; Anyasor GN; Osilesi O
[Ad] Endereço:Department of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, Babcock University, Ilishan-Remo, Ogun State, PMB, 21244 Ikeja, Nigeria. Electronic address: wumibukola@gmail.com.
[Ti] Título:Aqueous fraction of Alstonia boonei de Wild leaves suppressed inflammatory responses in carrageenan and formaldehyde induced arthritic rats.
[So] Source:Biomed Pharmacother;86:95-101, 2017 Feb.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Alstonia boonie de Wild is an ethnomedical plant used as therapy against inflammatory disorders. This study evaluated the most active anti-inflammatory and anti-oxidant fraction of A. boonei leaves using in vitro and in vivo models. Quantitative phytochemical analysis, anti-protein denaturation and hypotonicity-induced hemolysis of human red blood cell membrane (HRBC), radical scavenging activity assays, carrageenan and formaldehyde-induced inflammation models were carried out. Results showed that aqueous and ethyl acetate fractions of 70% methanol extract of A. boonie leaves contained high quantities of total phenolic and flavonoid compounds compared with hexane and butanol fractions. Aqueous fraction of A. boonie leaves significantly (P<0.05) inhibited heat-induced protein denaturation, stabilized hypotonicity-induced hemolysis of HRBC, scavenged DPPH, NO and H O radicals in a concentration-dependent manner compared with other fractions in vitro. In addition, orally administered 50-250-mg/kg body weight (b.w.) aqueous fraction of A. boonei leaves suppressed carrageenan-induced rat paw edema thickness by 74.32%, 79.22% and 89.86% respectively at 6th h in a dose-dependent manner comparable with animals treated with standard diclofenac sodium (88.69%) in vivo. Furthermore, investigation of formaldehyde-induced inflammation in rats showed that 50-250 mg/kg b.w. aqueous fraction of A. boonei reduced plasma alanine aminotransferase and aspartate aminotransferase activities. Aqueous fraction of A. boonei also suppressed eosinophils, monocytes and basophils, total white blood cell, total platelet, neutrophil and lymphocyte counts and modulated plasma lipid profile compared with control group. Aqueous fraction of A. boonei leaves exhibited substantial active anti-inflammatory and antioxidant activities. Hence, an aqueous fraction of A. boonei leaves could be channeled towards pharmaceutical drug development. In addition, this study provided scientific insight to account for the traditional use of A. boonei leaves in ethnomedical practice.
[Mh] Termos MeSH primário: Alstonia
Anti-Inflamatórios/uso terapêutico
Artrite Experimental/prevenção & controle
Carragenina/toxicidade
Formaldeído/toxicidade
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Artrite Experimental/induzido quimicamente
Artrite Experimental/metabolismo
Relação Dose-Resposta a Droga
Seres Humanos
Masculino
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Ratos
Ratos Wistar
Água/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 059QF0KO0R (Water); 1HG84L3525 (Formaldehyde); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE


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[PMID]:27721538
[Au] Autor:Barua AG; Raj H; Konch P; Hussain P; Barua CC
[Ad] Endereço:Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
[Ti] Título:Evaluation of antimycobacterial activity of some folklore medicinal plants and enumeration of colony forming unit in murine model.
[So] Source:Indian J Pharmacol;48(5):526-530, 2016 Sep-Oct.
[Is] ISSN:1998-3751
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The present study was carried out to investigate the antimycobacterial activity of methanol extract of and in murine model. MATERIALS AND METHODS: Female BALB/c mice were infected with the H Rv suspension. Extracts were administered orally for 2 weeks from 7 day postinfection at a dose of 200 mg/kg and rifampicin at 20 mg/kg as standard. The synergistic groups were 10 and 100 mg/kg for rifampicin and extract, respectively. RESULTS: The final body weight of mycobacteria-infected group was significantly reduced (15.41 ± 0.42, < 0.01), but following treatment with the plant extract plus rifampicin could elevate the body weight. Colony forming unit (CFU) count of lung (8.71 ± 0.01) and spleen (8.59 ± 0.01) was significantly higher in infected and untreated group ( < 0.01). It was observed that activity of the synergistic group displayed powerful and maximum response against tuberculosis (TB) infection with lower CFU counts. Histopathology study showed cells such as lymphocytes, epithelioid, Langhans giant cell, and fibrous tissue proliferation in lungs; depletion of lymphocytes in the spleen. CONCLUSIONS: The data indicate that methanol extract of has potential antimycobacterial activity, and the synergistic group consisting of rifampicin and could be a rational choice for the treatment of TB.
[Mh] Termos MeSH primário: Alstonia
Antituberculosos/uso terapêutico
Mucuna
Extratos Vegetais/uso terapêutico
Tuberculose/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Contagem de Colônia Microbiana
Feminino
Folclore
Pulmão/efeitos dos fármacos
Pulmão/microbiologia
Pulmão/patologia
Camundongos Endogâmicos BALB C
Mycobacterium tuberculosis/isolamento & purificação
Fitoterapia
Plantas Medicinais
Baço/efeitos dos fármacos
Baço/microbiologia
Tuberculose/microbiologia
Tuberculose/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


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[PMID]:26821000
[Au] Autor:Wang CM; Chen HT; Wu ZY; Jhan YL; Shyu CL; Chou CH
[Ad] Endereço:Research Center for Biodiversity, China Medical University, Taichung, 40402, Taiwan. wangchaomin@mail.cmu.edu.tw.
[Ti] Título:Antibacterial and Synergistic Activity of Pentacyclic Triterpenoids Isolated from Alstonia scholaris.
[So] Source:Molecules;21(2):139, 2016 Jan 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:(1) BACKGROUND: Alstonia scholaris (Apocynaceae) is an important medicinal plant that has been historically used in "Dai" ethnopharmacy to treat infectious diseases in China. Although various pharmacological activities have been reported, the antimicrobial constitutes of A. scholaris have not yet been identified. The objective of this study is to evaluate the antibacterial constitutes from the leaf extract of A. scholaris and to assess the synergistic effects of isolated compounds with antibiotics against bacterial pathogens.; (2) METHODS: The chemical constitutes isolated from the leaf extract of A. scholaris were structurally identified by NMR. The antibacterial and synergistic effect of compounds was assessed by calculating the minimal inhibitory concentration (MIC), checkerboard dilution test, and time-kill assay.; (3) RESULTS: Six pentacyclic triterpenoids were structurally identified as (1) lupeol, (2) betulin, (3) 3-hydroxy-11-ursen-28,13-olide, (4) betulinic acid, (5) oleanolic acid and (6) ursolic acid. Both oleanolic and ursolic acid showed antibacterial activity but were limited to Gram-positive bacteria. Ursolic acid showed a synergistic effect with ampicillin and tetracycline against both Bacillus cereus and S. aureus.; (4) CONCLUSION: These findings reflect that pentacyclic triterpenoids are the antibacterial chemicals in A. scholaris. The ability of ursolic acid to enhance the activity of antibiotics can constitute a valuable group of therapeutic agents in the future.
[Mh] Termos MeSH primário: Alstonia/química
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Triterpenos Pentacíclicos/isolamento & purificação
Triterpenos Pentacíclicos/farmacologia
[Mh] Termos MeSH secundário: Ampicilina/farmacologia
Bacillus cereus/efeitos dos fármacos
Sinergismo Farmacológico
Testes de Sensibilidade Microbiana
Estrutura Molecular
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
Staphylococcus aureus/efeitos dos fármacos
Tetraciclina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Pentacyclic Triterpenes); 0 (Plant Extracts); 7C782967RD (Ampicillin); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160129
[St] Status:MEDLINE


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[PMID]:26800290
[Au] Autor:Pan Z; Qin XJ; Liu YP; Wu T; Luo XD; Xia C
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, Yunnan, China.
[Ti] Título:Alstoscholarisines H-J, Indole Alkaloids from Alstonia scholaris: Structural Evaluation and Bioinspired Synthesis of Alstoscholarisine H.
[So] Source:Org Lett;18(4):654-7, 2016 Feb 19.
[Is] ISSN:1523-7052
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alstoscholarisines H-J (1-3), new monoterpenoid indole alkaloids with an unprecedented skeleton created via the formation of a C-3/N-1 bond, were isolated from Alstonia scholaris. Their structures were established by extensive spectroscopic analyses and the assessment of single-crystal X-ray diffraction data. The total synthesis of alstoscholarisine H was achieved via the regioselective nucleophilic addition of pyridinium through a bioinspired iminium ion intermediate followed by Pictet-Spengler-like cyclization.
[Mh] Termos MeSH primário: Alstonia/química
Alcaloides de Triptamina e Secologanina
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Conformação Molecular
Estrutura Molecular
Alcaloides de Triptamina e Secologanina/síntese química
Alcaloides de Triptamina e Secologanina/química
Alcaloides de Triptamina e Secologanina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Secologanin Tryptamine Alkaloids); 0 (alstoscholarisine H)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160219
[Lr] Data última revisão:
160219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160123
[St] Status:MEDLINE
[do] DOI:10.1021/acs.orglett.5b03583


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[PMID]:26707569
[Au] Autor:Zhao YL; Shang JH; Pu SB; Wang HS; Wang B; Liu L; Liu YP; Shen HM; Luo XD
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, China.
[Ti] Título:Effect of total alkaloids from Alstonia scholaris on airway inflammation in rats.
[So] Source:J Ethnopharmacol;178:258-65, 2016 Feb 03.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Alstonia scholaris (Apocynaceae) have been traditionally used for treatment of respiratory diseases in "dai" ethnopharmacy for hundreds years, especially for cough, asthma, phlegm, chronic obstructive pulmonary disease and so on. The formulas including the leaf extract have also been prescribed in hospitals and sold over the retail pharmacies. AIM OF THE STUDY: A. scholaris is used as a traditional herbal medicine for the treatment of respiratory tract inflammation. However, there is no scientific evidence to validate the use of total alkaloids of A. scholaris in the literature. Here, we investigated the protective activity of total alkaloids (TA), extracted from the leaves of Alstonia scholaris, against lipopolysaccharide (LPS)-induced airway inflammation (AI) in rats. MATERIALS AND METHODS: 200 µg/µL LPS was instilled intratracheally in each rat, and then the modeling animals were divided into six groups (n=10, each) randomly: sham group, LPS group, Dexamethasone [1.5mg/kg, intra-gastricly (i.g.)] group, and three different doses (7.5, 15, and 30 mg/kg, i.g.) of total alkaloids-treated groups. Corresponding drugs or vehicles were orally administered once per day for 7 days consecutively. The concentration of albumin (ALB), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), and the number of inflammatory cells in bronchoalveolar lavage fluid (BALF) were determined by fully automatic biochemical analyzer and blood counting instrument. Nitric oxide (NO) level, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activities were examined by multiskan spectrum, and histological change in the lungs was analyzed by H.E. staining. The levels of inflammatory cytokine tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) were measured using ELISA. RESULTS: Total alkaloids decreased the percentage of neutrophil, number of WBC, levels of ALB, AKP and LDH in the BALF, while increased the content of ALB in serum. It also improved SOD activity and increased NO level in the lungs, serum and BALF, and reduced the concentration of MDA in the lungs. Total alkaloids also inhibited the production of inflammatory cytokines TNF-α and IL-8 in the BALF and lung. Finally, histopathological examination indicated that total alkaloids attenuated tissue injury of the lungs in LPS-induced AI. CONCLUSIONS: Total alkaloids have an inhibitory effect against LPS-induced airway inflammation in rats.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Alstonia/química
Inflamação/tratamento farmacológico
Pulmão/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Animais
Líquido da Lavagem Broncoalveolar
Modelos Animais de Doenças
Inflamação/metabolismo
Interleucina-8/metabolismo
L-Lactato Desidrogenase/metabolismo
Pulmão/metabolismo
Masculino
Malondialdeído/metabolismo
Óxido Nítrico/metabolismo
Extratos Vegetais/química
Folhas de Planta/química
Ratos
Ratos Sprague-Dawley
Superóxido Dismutase/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Interleukin-8); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide); 4Y8F71G49Q (Malondialdehyde); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.15.1.1 (Superoxide Dismutase); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151229
[St] Status:MEDLINE


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[PMID]:26667977
[Au] Autor:Datta A; Moitra S; Hazra I; Mondal S; Das PK; Singh MK; Chaudhuri S; Bhattacharya D; Tripathi SK; Chaudhuri S
[Ad] Endereço:Department of Laboratory Medicine, School of Tropical Medicine, 108 C. R. Avenue, Kolkata 700073, West Bengal, India; Department of Clinical & Experimental Pharmacology, School of Tropical Medicine, 108 C. R. Avenue, Kolkata 700073, West Bengal, India.
[Ti] Título:Specific allergen immunotherapy attenuates allergic airway inflammation in a rat model of Alstonia scholaris pollen induced airway allergy.
[So] Source:Int Immunopharmacol;30:111-120, 2016 Jan.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pollen grains are well established to be an important cause of respiratory allergy. Current pharmacologic therapies for allergic asthma do not cure the disease. Allergen specific immunotherapy is the only treatment method which re-directs the immune system away from allergic response leading to a long lasting effect. The mechanism by which immunotherapy achieves this goal is an area of active research world-wide. The present experimental study was designed to develop an experimental model of allergic lung inflammation based on a relevant human allergen, Alstonia scholaris pollen, and to establish the immunological and cellular features of specific allergen immunotherapy using this same pollen extract. Our results revealed that Alstonia scholaris pollen sensitization and challenge causes eosinophilic airway inflammation with mucin hypersecretion. This is associated with increased total IgE, increased expression of FcɛRI on lung mast cells and increased levels of IL-4, IL-5 & IL-13 as confirmed by ELISA, in-situ immunofluorescence and FACS assay. Allergen specific immunotherapy reduced airway inflammation and also decreased total IgE level, FcɛRI expression, IL-4, IL-5 & IL-13 levels. It was further noted that the reduction of these levels was more by intra-nasal route than by intra-peritoneal route. Thus we present a novel animal model of Alstonia scholaris pollen allergic disease and specific allergen immunotherapy which will pave the way towards the development of better treatment modalities.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Dessensibilização Imunológica
Eosinófilos/imunologia
Mastócitos/imunologia
Pneumonia/terapia
Pólen/imunologia
Rinite Alérgica Sazonal/terapia
[Mh] Termos MeSH secundário: Administração Intranasal
Alstonia/imunologia
Animais
Células Cultivadas
Citocinas/metabolismo
Modelos Animais de Doenças
Seres Humanos
Imunoglobulina E/sangue
Mucinoses
Pneumonia/imunologia
Ratos
Ratos Wistar
Receptores de IgE/metabolismo
Rinite Alérgica Sazonal/imunologia
Células Th2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Allergens); 0 (Cytokines); 0 (FcepsilonRI protein, rat); 0 (Receptors, IgE); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170729
[Lr] Data última revisão:
170729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151216
[St] Status:MEDLINE



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