Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.456.500.131 [Categoria DeCS]
Referências encontradas : 61 [refinar]
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[PMID]:28213296
[Au] Autor:Li H; Liu Y; Yi Y; Miao Q; Liu S; Zhao F; Cong W; Wang C; Xia C
[Ad] Endereço:Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China; University of Chinese Academy of Sciences,19 Yuquan Road, Beijing 100049, PR China.
[Ti] Título:Purification of quercetin-3-O-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1048:56-63, 2017 Mar 24.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3-O-sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3-O-sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo-first-order and intraparticle diffusion kinetics models, the pseudo-second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively.
[Mh] Termos MeSH primário: Apocynum/química
Folhas de Planta/química
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Adsorção
Cromatografia Líquida de Alta Pressão/métodos
Dextranos/química
Extratos Vegetais/química
Quercetina/química
Quercetina/isolamento & purificação
Resinas Sintéticas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dextrans); 0 (Plant Extracts); 0 (Resins, Synthetic); 0YX10VRV6J (isoquercitrin); 18609-17-1 (quercetin-3-O-sophoroside); 9014-76-0 (sephadex); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


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[PMID]:26959002
[Au] Autor:Tan Z; Yi Y; Wang H; Zhou W; Wang C
[Ad] Endereço:Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. tanzhijian@caas.cn.
[Ti] Título:Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System.
[So] Source:Molecules;21(3):262, 2016 Mar 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ionic liquids (ILs) are considered as green solvents, and widely applied for the extraction of various compounds. METHODS: The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE). Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS) was applied for further isolation of flavonoids. RESULTS: The flavonoids were mainly distributed in the top phase, while impurities were extracted to the bottom phase. The parameters influencing the extraction, namely type and concentration of salt, temperature, and pH, were studied in detail. Under optimized conditions (72.43% IL extract, 28.57% (NH4)2SO4, 25 °C temperature, pH 4.5), the preconcentration factor and extraction efficiency were found to be 3.78% and 93.35%, respectively. CONCLUSIONS: This simple and efficient methodology is expected to see great use in the extraction and isolation of pharmaceutically active components from medicinal plant resources.
[Mh] Termos MeSH primário: Apocynum/química
Flavonoides/isolamento & purificação
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Líquidos Iônicos
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Plantas Medicinais/química
Solventes
Ultrassom
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Flavonoids); 0 (Ionic Liquids); 0 (Plant Extracts); 0 (Solvents); 059QF0KO0R (Water)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160310
[St] Status:MEDLINE


  3 / 61 MEDLINE  
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[PMID]:26935776
[Au] Autor:Liu XX; Tang L; Ge R; Li JK; Kang Y; Zhu MX; Li QS; Hao XL
[Ad] Endereço:Shanxi Institute of Traditional Chinese Medicine, Taiyuan, Shanxi 030012, P.R. China.
[Ti] Título:iTRAQ-based quantitative proteomic analysis of the anti-apoptotic effect of hyperin, which is mediated by Mcl-1 and Bid, in H2O2-injured EA.hy926 cells.
[So] Source:Int J Mol Med;37(4):1083-90, 2016 Apr.
[Is] ISSN:1791-244X
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Endothelial injury has been implicated in the pathogenesis of many cardiovascular diseases, including thrombotic disorders. Hyperin (quercetin-3-O-galactoside), a flavonoid compound and major bioactive component of the medicinal herb Apocynum venetum L., is commonly used to prevent endothelium dysfunction. However, its mode of action remains unclear. To the best of our knowledge, we have for the first time investigated the protective effect hyperin exerts against H2O2-induced injury in human endothelium-derived EA.hy926 cells using isobaric tags for relative and absolute quantitation (iTRAQ)­based quantitative proteomic analysis. The results showed that H2O2 exposure induced alterations in the expression of 250 proteins in the cells. We noted that the expression of 52 proteins associated with processes such as cell apoptosis, cell cycle and cytoskeleton organization, was restored by hyperin treatment. Of the proteins differentially regulated following H2O2 stress, the anti-apoptotic protein, myeloid cell leukemia-1 (Mcl-1), and the pro-apoptotic protein, BH3-interacting domain death agonist (Bid), exhibited marked changes in expression. Hyperin increased Mcl-1 expression and decreased that of Bid in a dose-dependent manner. In addition, flow cytometric analysis and western blot analysis of the apoptosis-related proteins, truncated BID (tBid), cleaved caspase-3, cleaved caspase-9, Fas, FasL and caspase-8, demonstrated that the rate of apoptosis and the pro-apoptotic protein levels were decreased by hyperin pre­treatment. In the present study we demonstrate that hyperin effectively prevents H2O2­induced cell injury by regulating the Mcl­1­ and Bid-mediated anti­apoptotic mechanism, suggesting that hyperin is a potential candidate for use in the treatment of thrombotic diseases.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Proteína Agonista de Morte Celular de Domínio Interatuante com BH3/metabolismo
Endotélio/efeitos dos fármacos
Proteína de Sequência 1 de Leucemia de Células Mieloides/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Apocynum/química
Caspase 3/metabolismo
Caspase 8/metabolismo
Caspase 9/metabolismo
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular
Endotélio/citologia
Endotélio/metabolismo
Seres Humanos
Peróxido de Hidrogênio/metabolismo
Substâncias Protetoras/química
Proteômica
Quercetina/química
Quercetina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (BH3 Interacting Domain Death Agonist Protein); 0 (BID protein, human); 0 (MCL1 protein, human); 0 (Myeloid Cell Leukemia Sequence 1 Protein); 0 (Protective Agents); 8O1CR18L82 (hyperoside); 9IKM0I5T1E (Quercetin); BBX060AN9V (Hydrogen Peroxide); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 8); EC 3.4.22.- (Caspase 9)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160304
[St] Status:MEDLINE
[do] DOI:10.3892/ijmm.2016.2510


  4 / 61 MEDLINE  
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[PMID]:26133970
[Au] Autor:Lau YS; Ling WC; Murugan D; Kwan CY; Mustafa MR
[Ad] Endereço:Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. lauyehsiang@um.edu.my.
[Ti] Título:Endothelium-Dependent Relaxation Effect of Apocynum venetum Leaf Extract via Src/PI3K/Akt Signalling Pathway.
[So] Source:Nutrients;7(7):5239-53, 2015 Jun 30.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Botanical herbs are consumed globally not only as an essential diet but also as medicines or as functional/recreational food supplements. The extract of the Apocynum venetum leaves (AVLE), also known as Luobuma, exerts its antihypertensive effect via dilating the blood vessels in an endothelium- and concentration-dependent manner with optimal effect seen at as low as 10 µg/mL. A commercial Luoboma "antihypertensive tea" is available commercially in the western province of China. The present study seeks to investigate the underlying cellular mechanisms of the nitric oxide (NO)-releasing property of AVLE in rat aortas and human umbilical vein endothelial cells (HUVECs). Endothelium-dependent relaxation induced by AVLE was assessed in organ chambers in the presence or absence of polyethyleneglycol catalase (PP2, 20 µM; inhibitor of Src kinase), wortmannin (30 nM) and LY294002 (20 µM; PI3 (phosphatidylinositol3)-Kinase inhibitor), N(G)-nitro-L-arginine (L-NAME, 100 µM; endothelial NO synthase inhibitor (eNOS)) and ODQ (1 µM; soluble guanylyl cyclase inhibitor). Total nitrite and nitrate (NOx) level and protein expression of p-Akt and p-eNOS were measured. AVLE-induced endothelium-dependent relaxation was reduced by PP2, wortmannin and LY294002 and abolished by L-NAME and ODQ. AVLE significantly increased total NOx level in rat aortas and in HUVECs compared to control. It also instigated phosphorylation of Akt and eNOS in cultured HUVECs in a concentration-dependent manner and this was markedly suppressed by PP2, wortmannin and LY294002. AVLE also inhibited superoxide generated from both NADPH oxidase and xanthine/xanthine oxidase system. Taken together, AVLE causes endothelium-dependent NO mediated relaxations of rat aortas through Src/PI3K/Akt dependent NO signalling pathway and possesses superoxide scavenging activity.
[Mh] Termos MeSH primário: Apocynum/química
Medicamentos de Ervas Chinesas/farmacologia
Endotélio Vascular/efeitos dos fármacos
Folhas de Planta/química
Transdução de Sinais/efeitos dos fármacos
Vasodilatação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Aorta
Endotélio Vascular/fisiologia
Seres Humanos
Masculino
NADPH Oxidases/metabolismo
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo III/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação
Proteínas Proto-Oncogênicas c-akt/metabolismo
Ratos
Ratos Sprague-Dawley
Veias Umbilicais/metabolismo
Vasodilatação/fisiologia
Quinases da Família src/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type III); EC 1.6.3.- (NADPH Oxidases); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.10.2 (src-Family Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150703
[St] Status:MEDLINE
[do] DOI:10.3390/nu7075220


  5 / 61 MEDLINE  
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[PMID]:26052150
[Au] Autor:Yamatsu A; Yamashita Y; Maru I; Yang J; Tatsuzaki J; Kim M
[Ad] Endereço:Pharma Foods International Co., Ltd.
[Ti] Título:The Improvement of Sleep by Oral Intake of GABA and Apocynum venetum Leaf Extract.
[So] Source:J Nutr Sci Vitaminol (Tokyo);61(2):182-7, 2015.
[Is] ISSN:1881-7742
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The effects of two food materials, γ-aminobutyric acid (GABA) produced by natural fermentation and Apocynum venetum leaf extract (AVLE), on the improvement of sleep were investigated in humans. The electroencephalogram (EEG) test revealed that oral administration of GABA (100 mg) and AVLE (50 mg) had beneficial effects on sleep. GABA shortened sleep latency by 5.3 min and AVLE increased non-rapid eye movement (REM) sleep time by 7.6%. Simultaneous intake of GABA and AVLE shortened sleep latency by 4.3 min and increased non-REM sleep time by 5.1%. The result of questionnaires showed that GABA and AVLE enabled subjects to realize the effects on sleep. These results mean that GABA can help people to fall asleep quickly, AVLE induces deep sleep, and they function complementarily with simultaneous intake. Since both GABA and AVLE are materials of foods and have been ingested for a long time, they can be regarded as safe and appropriate for daily intake in order to improve the quality of sleep.
[Mh] Termos MeSH primário: Apocynum
Extratos Vegetais/uso terapêutico
Transtornos do Sono-Vigília/tratamento farmacológico
Sono/efeitos dos fármacos
Ácido gama-Aminobutírico/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Dieta
Eletroencefalografia
Feminino
Fermentação
Seres Humanos
Masculino
Fitoterapia
Extratos Vegetais/farmacologia
Folhas de Planta/química
Sono REM
Inquéritos e Questionários
Ácido gama-Aminobutírico/farmacologia
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 56-12-2 (gamma-Aminobutyric Acid)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150608
[Lr] Data última revisão:
150608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150609
[St] Status:MEDLINE
[do] DOI:10.3177/jnsv.61.182


  6 / 61 MEDLINE  
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[PMID]:26021846
[Au] Autor:Song Rj; Zhou J
[Ad] Endereço:School of Chemistry and Chemical Engineering, Xi'an University, Xi'an, Shanxi 710065, China.
[Ti] Título:Microemulsion liquid chromatographic method for simultaneous separation and determination of six flavonoids of Apocynum venetum leaf extract.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;995-996:8-14, 2015 Jul 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple, cost-effective, and efficient method was developed for the rapid simultaneous separation and determination of six flavonoids (rutin, hyperoside, quercetin-3-O-sophoroside, isoquercitrin, astragalin and quercetin) of Apocynum venetum leaf extract by reversed phase high performance liquid chromatography using a microemulsion system mixture as the mobile phase. Separations were performed on the Zorbax Extend-C18 column with UV detection at 360nm. The flow rate was 0.8mLmin(-1). The optimized microemulsion mobile phase consisted of 2.5% (v/v) n-butanol, 1.2% (v/v) of Genapol X-080, 0.5% (v/v) ethyl acetate and 95.8% (w/v) of aqueous 20mM phosphoric acid, pH adjusted to 6.0 with 0.3% triethylamine. Under the optimized conditions, the calibration curve for six flavonoids was linear in the range of 5-1000µgmL(-1) with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.11% and the limits of detection (LOD) for the six flavonoids were 1.7-6.0µgmL(-1) (S/N=3). The microemulsion liquid chromatography (MELC) method was successfully applied to separate and determine the six flavonoids of A. venetum leaf extract.
[Mh] Termos MeSH primário: Apocynum/química
Cromatografia Líquida de Alta Pressão/métodos
Flavonoides/análise
[Mh] Termos MeSH secundário: Emulsões
Flavonoides/química
Flavonoides/isolamento & purificação
Limite de Detecção
Modelos Lineares
Extratos Vegetais/química
Folhas de Planta/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Emulsions); 0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150616
[Lr] Data última revisão:
150616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150530
[St] Status:MEDLINE


  7 / 61 MEDLINE  
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[PMID]:25967663
[Au] Autor:Xie W; Chen C; Jiang Z; Wang J; Melzig MF; Zhang X
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
[Ti] Título:Apocynum venetum Attenuates Acetaminophen-Induced Liver Injury in Mice.
[So] Source:Am J Chin Med;43(3):457-76, 2015.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Apocynum venetum L. (A. venetum) has long been used in oriental folk medicine for the treatment of some liver diseases; however, the underlying mechanisms remain to be fully elucidated. Acetaminophen (APAP) is a widely used analgesic drug that can cause acute liver injury in overdose situations. In this study, we investigated the potential protective effect of A. venetum leaf extract (ALE) against APAP-induced hepatotoxicity. Mice were intragastrically administered with ALE once daily for 3 consecutive days prior to receiving a single intraperitoneal injection of APAP. The APAP group showed severe liver injury characterized by the noticeable fluctuations in the following parameters: serum aminotransferases; hepatic malondialdehyde (MDA), 3-nitrotyrosine (3-NT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione (GSH). These liver damages induced by APAP were significantly attenuated by ALE pretreatments. A collective analysis of histopathological examination, DNA laddering and western blot for caspase-3 and cytochrome c indicated that the ALE is also capable of preventing APAP-induced hepatocyte death. Hyperoside, isoquercitrin and their derivatives have been identified as the major components of ALE using HPLC-MS/MS. Taken together, the A. venetum possesses hepatoprotective effects partially due to its anti-oxidant action.
[Mh] Termos MeSH primário: Acetaminofen
Antioxidantes
Apocynum
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Fitoterapia
Extratos Vegetais/administração & dosagem
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Doença Hepática Induzida por Substâncias e Drogas/patologia
Hepatócitos/efeitos dos fármacos
Hepatócitos/patologia
Injeções Intraperitoneais
Masculino
Camundongos Endogâmicos
Estresse Oxidativo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 362O9ITL9D (Acetaminophen)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X15500299


  8 / 61 MEDLINE  
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[PMID]:25835371
[Au] Autor:Kasimu R; Fan Z; Wang X; Hu J; Wang P; Wang J
[Ad] Endereço:College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China. Electronic address: renakasimu@163.com.
[Ti] Título:Anti-platelet aggregation activities of different fractions in leaves of Apocynum venetum L.
[So] Source:J Ethnopharmacol;168:116-21, 2015 Jun 20.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Ethnopharmacological relevance Apocynum venetum: is widely used in Uygur and traditional Chinese medicine. Modern pharmaceutical studies have shown that leaves of A. venetum have effects of liver protection, antidepressant and regulation of blood pressure. However, it is unclear that which components have pharmacological activities. AIM OF THE STUDY: The aim was to study chemical constituents of A. venetum and its anti-platelet aggregation activity. MATERIALS AND METHODS: Nephelometery was applied to evaluate anti-platelet aggregation activity of multi-components of A. venetum. Systematic separation components were characterized by HPLC analysis method, and in vitro screening active components by anti-platelet aggregation study. RESULTS: Ethyl acetate fraction (L-III) and L-III-4 have better anti-platelet aggregation activity than other fractions. The results indicated that isoquercitrin, hyperoside and other flavonoids have anti-platelet aggregation activity in A. venetum. CONCLUSION: Our studies provide basis on the endeavors of screening chemicals with that anti-platelet aggregation activity in leaves of A. venetum.
[Mh] Termos MeSH primário: Apocynum
Extratos Vegetais/farmacologia
Inibidores da Agregação de Plaquetas/farmacologia
[Mh] Termos MeSH secundário: Acetatos/química
Animais
Flavonoides/farmacologia
Masculino
Folhas de Planta
Agregação Plaquetária/efeitos dos fármacos
Coelhos
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Flavonoids); 0 (Plant Extracts); 0 (Platelet Aggregation Inhibitors); 0 (Solvents); 76845O8NMZ (ethyl acetate)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150524
[Lr] Data última revisão:
150524
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150404
[St] Status:MEDLINE


  9 / 61 MEDLINE  
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[PMID]:25625477
[Au] Autor:Zhou J; Sun JB; Xu XY; Cheng ZH; Zeng P; Wang FQ; Zhang Q
[Ad] Endereço:Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
[Ti] Título:Application of mixed cloud point extraction for the analysis of six flavonoids in Apocynum venetum leaf samples by high performance liquid chromatography.
[So] Source:J Pharm Biomed Anal;107:273-9, 2015 Mar 25.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A simple, inexpensive and efficient method based on the mixed cloud point extraction (MCPE) combined with high performance liquid chromatography was developed for the simultaneous separation and determination of six flavonoids (rutin, hyperoside, quercetin-3-O-sophoroside, isoquercitrin, astragalin and quercetin) in Apocynum venetum leaf samples. The non-ionic surfactant Genapol X-080 and cetyl-trimethyl ammonium bromide (CTAB) was chosen as the mixed extracting solvent. Parameters that affect the MCPE processes, such as the content of Genapol X-080 and CTAB, pH, salt content, extraction temperature and time were investigated and optimized. Under the optimized conditions, the calibration curve for six flavonoids were all linear with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.1% and the limits of detection (LOD) for the six flavonoids were 1.2-5.0 ng mL(-1) (S/N=3). The proposed method was successfully used to separate and determine the six flavonoids in A. venetum leaf samples.
[Mh] Termos MeSH primário: Apocynum/química
Flavonoides/química
Extratos Vegetais/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Compostos de Cetrimônio/química
Cromatografia Líquida de Alta Pressão/métodos
Concentração de Íons de Hidrogênio
Quempferóis/química
Polietilenoglicóis/química
Quercetina/análogos & derivados
Quercetina/química
Rutina/química
Tensoativos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Flavonoids); 0 (Kaempferols); 0 (Plant Extracts); 0 (Surface-Active Agents); 0YX10VRV6J (isoquercitrin); 18609-17-1 (quercetin-3-O-sophoroside); 30IQX730WE (Polyethylene Glycols); 5G06TVY3R7 (Rutin); 8O1CR18L82 (hyperoside); 9043-30-5 (genapol X 080); 9IKM0I5T1E (Quercetin); APM8UQ3Z9O (astragalin); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150128
[St] Status:MEDLINE


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[PMID]:25579758
[Au] Autor:Wang W; Liang X; Fu D; Tie R; Xing W; Ji L; Liu F; Zhang H; Li R
[Ad] Endereço:Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, P. R. China , Experiment Teaching Center, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, P. R. China.
[Ti] Título:Apocynum venetum leaf attenuates myocardial ischemia/reperfusion injury by inhibiting oxidative stress.
[So] Source:Am J Chin Med;43(1):71-85, 2015.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Apocynum venetum, a Chinese medicinal herb, is reported to be neuroprotective. However, whether Apocynum venetum leaf extract (AVLE) protects against ischemic myocardium remains elusive. Our present study was aimed to observe the effects of AVLE preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to investigate the possible mechanisms. Rats were treated with AVLE (500 mg/kg/d, o.g.) or distilled water once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. AVLE preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, AVLE reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, AVLE preconditioning significantly inhibited superoxide generation, gp91(phox) expression, malonaldialdehyde formation and enhanced superoxide dismutase (SOD) activity in I/R hearts. Furthermore, AVLE treatment increased Akt and extracellular regulated protein kinases 1/2 (ERK1/2) phosphorylations in I/R rat heart. Either the Phosphatidylinositide 3-kinase (PI3K) inhibitor wortmannin or the ERK1/2 inhibitor PD98059 blocked AVLE-stimulated anti-oxidative effects and cardioprotection. Our study demonstrated for the first time that AVLE reduces oxidative stress and exerts cardioprotection against MI/R injury in rats.
[Mh] Termos MeSH primário: Antioxidantes
Apocynum
Medicamentos de Ervas Chinesas/farmacologia
Medicamentos de Ervas Chinesas/uso terapêutico
Isquemia Miocárdica/prevenção & controle
Traumatismo por Reperfusão Miocárdica/prevenção & controle
Estresse Oxidativo/efeitos dos fármacos
Fitoterapia
[Mh] Termos MeSH secundário: Androstadienos/farmacologia
Apoptose
Creatina Quinase/sangue
Medicamentos de Ervas Chinesas/administração & dosagem
Flavonoides/farmacologia
Precondicionamento Isquêmico
L-Lactato Desidrogenase/sangue
Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Isquemia Miocárdica/enzimologia
Isquemia Miocárdica/patologia
Traumatismo por Reperfusão Miocárdica/enzimologia
Traumatismo por Reperfusão Miocárdica/patologia
Miocárdio/patologia
Fármacos Neuroprotetores
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Fosforilação/efeitos dos fármacos
Folhas de Planta
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one); 0 (Androstadienes); 0 (Antioxidants); 0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Neuroprotective Agents); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 2.7.3.2 (Creatine Kinase); XVA4O219QW (wortmannin)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150113
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X15500056



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