Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.456.500.260 [Categoria DeCS]
Referências encontradas : 508 [refinar]
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[PMID]:28701463
[Au] Autor:Kries H; Kellner F; Kamileen MO; O'Connor SE
[Ad] Endereço:From the Department of Biological Chemistry, The John Innes Centre, Norwich NR4 7UH, United Kingdom and.
[Ti] Título:Inverted stereocontrol of iridoid synthase in snapdragon.
[So] Source:J Biol Chem;292(35):14659-14667, 2017 Sep 01.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The natural product class of iridoids, found in various species of flowering plants, harbors astonishing chemical complexity. The discovery of iridoid biosynthetic genes in the medicinal plant has provided insight into the biosynthetic origins of this class of natural product. However, not all iridoids share the exact five- to six-bicyclic ring scaffold of the iridoids. For instance, iridoids in the ornamental flower snapdragon ( , Plantaginaceae family) are derived from the C7 epimer of this scaffold. Here we have cloned and characterized the iridoid synthase enzyme from (AmISY), the enzyme that is responsible for converting 8-oxogeranial into the bicyclic iridoid scaffold in a two-step reduction-cyclization sequence. Chiral analysis of the reaction products reveals that AmISY reduces C7 to generate the opposite stereoconfiguration in comparison with the homologue CrISY. The catalytic activity of AmISY thus explains the biosynthesis of 7-epi-iridoids in and related genera. However, although the stereoselectivity of the reduction step catalyzed by AmISY is clear, in both AmISY and CrISY, the cyclization step produces a diastereomeric mixture. Although the reduction of 8-oxogeranial is clearly enzymatically catalyzed, the cyclization step appears to be subject to less stringent enzyme control.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo
Antirrhinum/enzimologia
Iridoides/metabolismo
Modelos Moleculares
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/química
Alquil e Aril Transferases/genética
Substituição de Aminoácidos
Biocatálise
Domínio Catalítico
Catharanthus/enzimologia
Iridoides/química
Estrutura Molecular
Monoterpenos/química
Monoterpenos/metabolismo
Mutação
NADP/química
NADP/metabolismo
Oxirredução
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Conformação Proteica
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Estereoisomerismo
Homologia Estrutural de Proteína
Especificidade por Substrato
Terpenos/química
Terpenos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iridoids); 0 (Monoterpenes); 0 (Plant Proteins); 0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 0 (Terpenes); 53-59-8 (NADP); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase); PL902IKN0A (decaprenoic acid); T7EU0O9VPP (citral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.800979


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[PMID]:27890577
[Au] Autor:Bahieldin A; Atef A; Shokry AM; Al-Karim S; Al Attas SG; Gadallah NO; Edris S; Al-Kordy MA; Hassan SM; Abo-Aba S; El-Domyati FM
[Ad] Endereço:Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: bahieldin55@gmail.com.
[Ti] Título:Transcription factors regulating uspA genes in Catharanthus roseus.
[So] Source:C R Biol;340(1):1-6, 2017 Jan.
[Is] ISSN:1768-3238
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethylene-responsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses.
[Mh] Termos MeSH primário: Catharanthus/genética
Genes de Plantas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Simulação por Computador
Bases de Dados Genéticas
Regulação da Expressão Gênica de Plantas
RNA de Plantas/genética
Reação em Cadeia da Polimerase em Tempo Real
Estresse Fisiológico/genética
Regulação para Cima/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Plant); 0 (Transcription Factors)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE


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[PMID]:27819493
[Au] Autor:Khan WU; Ahmad SR; Yasin NA; Ali A; Ahmad A
[Ad] Endereço:a College of Earth and Environmental Sciences , University of the Punjab , Lahore , Pakistan.
[Ti] Título:Effect of Pseudomonas fluorescens RB4 and Bacillus subtilis 189 on the phytoremediation potential of Catharanthus roseus (L.) in Cu and Pb-contaminated soils.
[So] Source:Int J Phytoremediation;19(6):514-521, 2017 Jun 03.
[Is] ISSN:1549-7879
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The remediation of heavy metal-contaminated soils has become a critical issue due to toxic effects of these metals on living organisms. The current research was conducted to study the effect of Pseudomonas fluorescens RB4 and Bacillus subtilis 189 on the growth and phytoremediation potential of Catharanthus roseus in Cu- and Pb-contaminated soils. The bacterial strains exhibited significantly higher level of water-extractable Pb and Cu in Pb, Cu, and Cu+Pb-contaminated. The P. fluorescens RB4 inoculated plants, produced 102%, 48%, and 45% higher fresh weight (FW) in soils contaminated with Cu, Pb, and both elements, respectively, as compared to un-inoculated control plants. Similarly, B. subtilis 189 inoculated plants produced 108%, 43%, and 114% more FW in the presence of Cu, Pb, and both elements. The plants co-cultivated with both bacteria exhibited 121%, 102%, and 177% higher FW, in Cu, Pb, and both elements contaminated soils, as compared to respective un-inoculated control. Co-cultivation of P. fluorescens RB4, B. subtilis 189, and P. fluorescens RB4 + B. subtilis 189 resulted in higher accumulation of Cu and Pb in shoots of the C. roseus grown in contaminated soils as compared to un-inoculated control. Bacterial treatments also improved the translocation and metal bioconcentration factors. The growth and phytoextraction capability of C. roseus was improved by inoculation of P. fluorescens RB4 and B. subtilis 189.
[Mh] Termos MeSH primário: Bacillus subtilis/fisiologia
Catharanthus/metabolismo
Cobre/metabolismo
Chumbo/metabolismo
Pseudomonas fluorescens/fisiologia
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Catharanthus/crescimento & desenvolvimento
Catharanthus/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 2P299V784P (Lead); 789U1901C5 (Copper)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE
[do] DOI:10.1080/15226514.2016.1254154


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[PMID]:28036025
[Au] Autor:Liu J; Cai J; Wang R; Yang S
[Ad] Endereço:College of Chinese Herbal Medicine, Jilin Agricultural University, Changchun 130047, China. liujiaqi0817@163.com.
[Ti] Título:Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions.
[So] Source:Int J Mol Sci;18(1), 2016 Dec 28.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:As one of the model medicinal plants for exploration of biochemical pathways and molecular biological questions on complex metabolic pathways, synthesizes more than 100 terpenoid indole alkaloids (TIAs) used for clinical treatment of various diseases and for new drug discovery. Given that extensive studies have revealed the major metabolic pathways and the spatial-temporal biosynthesis of TIA in plant, little is known about subcellular and inter-cellular trafficking or long-distance transport of TIA end products or intermediates, as well as their regulation. While these transport processes are indispensable for multi-organelle, -tissue and -cell biosynthesis, storage and their functions, great efforts have been made to explore these dynamic cellular processes. Progress has been made in past decades on transcriptional regulation of TIA biosynthesis by transcription factors as either activators or repressors; recent studies also revealed several transporters involved in subcellular and inter-cellular TIA trafficking. However, many details and the regulatory network for controlling the tissue-or cell-specific biosynthesis, transport and storage of serpentine and ajmalicine in root, catharanthine in leaf and root, vindoline specifically in leaf and vinblastine and vincristine only in green leaf and their biosynthetic intermediates remain to be determined. This review is to summarize the progress made in biosynthesis, transcriptional regulation and transport of TIAs. Based on analysis of organelle, tissue and cell-type specific biosynthesis and progresses in transport and trafficking of similar natural products, the transporters that might be involved in transport of TIAs and their synthetic intermediates are discussed; according to transcriptome analysis and bioinformatic approaches, the transcription factors that might be involved in TIA biosynthesis are analyzed. Further discussion is made on a broad context of transcriptional and transport regulation in order to guide our future research.
[Mh] Termos MeSH primário: Catharanthus/metabolismo
Alcaloides de Triptamina e Secologanina/metabolismo
Ativação Transcricional
[Mh] Termos MeSH secundário: Transporte Biológico
Catharanthus/genética
Regulação da Expressão Gênica de Plantas
Redes Reguladoras de Genes
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Secologanin Tryptamine Alkaloids)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE


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[PMID]:27816224
[Au] Autor:Kotland A; Chollet S; Diard C; Autret JM; Meucci J; Renault JH; Marchal L
[Ad] Endereço:GEPEA, UMR CNRS 6144, University of Nantes, 37 bd de l'Université 44602 Saint Nazaire Cedex, France; ICMR, UMR CNRS 7312, University of Reims Champagne-Ardenne, France.
[Ti] Título:Industrial case study on alkaloids purification by pH-zone refining centrifugal partition chromatography.
[So] Source:J Chromatogr A;1474:59-70, 2016 Nov 25.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The industrial potential of pH-zone refining centrifugal partition chromatography has been evaluated by studying the purification of pharmaceutical ingredients at the pilot scale. For the first time, a scale up methodology based on both column capacity and mass transfer efficiency as invariants was developed. The purification of catharanthine and vindoline from an industrial crude extract of aerial parts of Catharanthus roseus, was used as a case of study. Toluene/CH CN/water (4/1/5, v/v/v) was selected as biphasic solvent system, triethylamine as retainer in the organic stationary phase and sulphuric acid as displacer in the aqueous mobile phase. The separation intensification was performed on a 36mL CPC column equipped with 832 partition twin-cells. The combined effects of four parameters (displacer and retainer concentrations for intensive parameters, flow rate and rotational speed for extensive parameters) were studied by design of experiment in order to maximize both recoveries and productivities. Then, scale change was done on two larger columns (305mL and 1950mL of capacity) equipped with only 231 and 238 partition cells. For this step, it has been shown that the global mass transfer coefficient k a (the efficiency of a column design) and the stationary phase retention (the capacity of the column) were relevant and useful scale up invariants. A CPC model based on acid-base equilibriums and interfacial mass transfer in continuously stirred tank reactors in series was used to predict fully separations on larger CPC column at the optimized operating conditions and to guide the CPC user in its scale-up strategy. The experimental validation on pilot CPC column, by injecting up to 150g of Catharanthus roseus crude extract on the 1950mL column highlighted the preservation of the separation quality, the non-linear character of the scale up in centrifugal partition chromatography and that a productivity of about 4kg of processed crude extract per day can be reached by implementing developed methodology.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
[Mh] Termos MeSH secundário: Algoritmos
Catharanthus/química
Centrifugação
Cromatografia Líquida de Alta Pressão
Distribuição Contracorrente/métodos
Concentração de Íons de Hidrogênio
Folhas de Planta/química
Solventes
Vimblastina/análogos & derivados
Vimblastina/isolamento & purificação
Alcaloides de Vinca/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Solvents); 0 (Vinca Alkaloids); 571PJ1LW03 (vindoline); 5V9KLZ54CY (Vinblastine); WT0YJV846J (catharanthine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27694525
[Au] Autor:Mertens J; Van Moerkercke A; Vanden Bossche R; Pollier J; Goossens A
[Ad] Endereço:Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium.
[Ti] Título:Clade IVa Basic Helix-Loop-Helix Transcription Factors Form Part of a Conserved Jasmonate Signaling Circuit for the Regulation of Bioactive Plant Terpenoid Biosynthesis.
[So] Source:Plant Cell Physiol;57(12):2564-2575, 2016 Dec.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Plants produce many bioactive, specialized metabolites to defend themselves when facing various stress situations. Their biosynthesis is directed by a tightly controlled regulatory circuit that is elicited by phytohormones such as jasmonate (JA). The basic helix-loop-helix (bHLH) transcription factors (TFs) bHLH iridoid synthesis 1 (BIS1) and Triterpene Saponin Activating Regulator (TSAR) 1 and 2, from Catharanthus roseus and Medicago truncatula, respectively, all belong to clade IVa of the bHLH protein family and activate distinct terpenoid pathways, thereby mediating monoterpenoid indole alkaloid (MIA) and triterpene saponin (TS) accumulation, respectively, in these two species. In this study, we report that promoters of the genes encoding the enzymes involved in the specific terpenoid pathway of one of these species can be transactivated by the orthologous bHLH factor from the other species through recognition of the same cis-regulatory elements. Accordingly, ectopic expression of CrBIS1 in M. truncatula hairy roots up-regulated the expression of all genes required for soyasaponin production, resulting in strongly increased levels of soyasaponins in the transformed roots. Likewise, transient expression of MtTSAR1 and MtTSAR2 in C. roseus petals led to up-regulation of the genes involved in the iridoid branch of the MIA pathway. Together, our data illustrate the functional similarity of these JA-inducible TFs and indicate that recruitment of defined cis-regulatory elements constitutes an important aspect of the evolution of conserved regulatory modules for the activation of species-specific terpenoid biosynthesis pathways by common signals such as the JA phytohormones.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Catharanthus/genética
Ciclopentanos/metabolismo
Medicago truncatula/genética
Oxilipinas/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Terpenos/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Catharanthus/fisiologia
Flores/genética
Flores/fisiologia
Regulação da Expressão Gênica de Plantas
Medicago truncatula/fisiologia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/genética
Raízes de Plantas/fisiologia
Plantas Geneticamente Modificadas
Regiões Promotoras Genéticas/genética
Saponinas/metabolismo
Transdução de Sinais
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Cyclopentanes); 0 (Oxylipins); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Saponins); 0 (Terpenes); 6RI5N05OWW (jasmonic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:27688619
[Au] Autor:Parage C; Foureau E; Kellner F; Burlat V; Mahroug S; Lanoue A; Dugé de Bernonville T; Londono MA; Carqueijeiro I; Oudin A; Besseau S; Papon N; Glévarec G; Atehortùa L; Giglioli-Guivarc'h N; St-Pierre B; Clastre M; O'Connor SE; Courdavault V
[Ad] Endereço:Université François-Rabelais de Tours, EA2106 Biomolécules et Biotechnologies Végétales, F-37200 Tours, France (C.P., E.F., S.M., A.L., T.D.d.B., M.A.L., I.C., A.O., S.B., G.G., N.G.-G., B.S.-P., M.C., V.C.).
[Ti] Título:Class II Cytochrome P450 Reductase Governs the Biosynthesis of Alkaloids.
[So] Source:Plant Physiol;172(3):1563-1577, 2016 Nov.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Expansion of the biosynthesis of plant specialized metabolites notably results from the massive recruitment of cytochrome P450s that catalyze multiple types of conversion of biosynthetic intermediates. For catalysis, P450s require a two-electron transfer catalyzed by shared cytochrome P450 oxidoreductases (CPRs), making these auxiliary proteins an essential component of specialized metabolism. CPR isoforms usually group into two distinct classes with different proposed roles, namely involvement in primary and basal specialized metabolisms for class I and inducible specialized metabolism for class II. By studying the role of CPRs in the biosynthesis of monoterpene indole alkaloids, we provide compelling evidence of an operational specialization of CPR isoforms in Catharanthus roseus (Madagascar periwinkle). Global analyses of gene expression correlation combined with transcript localization in specific leaf tissues and gene-silencing experiments of both classes of CPR all point to the strict requirement of class II CPRs for monoterpene indole alkaloid biosynthesis with a minimal or null role of class I. Direct assays of interaction and reduction of P450s in vitro, however, showed that both classes of CPR performed equally well. Such high specialization of class II CPRs in planta highlights the evolutionary strategy that ensures an efficient reduction of P450s in specialized metabolism.
[Mh] Termos MeSH primário: Alcaloides/biossíntese
Vias Biossintéticas
Catharanthus/enzimologia
NADPH-Ferri-Hemoproteína Redutase/metabolismo
[Mh] Termos MeSH secundário: Biocatálise
Vias Biossintéticas/genética
Catharanthus/genética
Cotilédone/metabolismo
Retículo Endoplasmático/metabolismo
Regulação da Expressão Gênica de Plantas
Inativação Gênica
Genes de Plantas
Alcaloides de Indol/metabolismo
NADPH-Ferri-Hemoproteína Redutase/química
NADPH-Ferri-Hemoproteína Redutase/genética
Folhas de Planta/metabolismo
Ligação Proteica
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Análise de Sequência de Proteína
Homologia de Sequência de Aminoácidos
Frações Subcelulares/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Indole Alkaloids); 0 (RNA, Messenger); EC 1.6.2.4 (NADPH-Ferrihemoprotein Reductase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


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[PMID]:27620272
[Au] Autor:Killiny N
[Ad] Endereço:Department of Plant Pathology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, USA. Electronic address: nabilkilliny@ufl.edu.
[Ti] Título:Generous hosts: What makes Madagascar periwinkle (Catharanthus roseus) the perfect experimental host plant for fastidious bacteria?
[So] Source:Plant Physiol Biochem;109:28-35, 2016 Dec.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Although much attention has been paid to the metabolism and biosynthesis of monoterpene alkaloids in Catharanthus roseus, its value as an experimental host for a variety of agriculturally and economically important phytopathogenic bacteria warrants further study. In the present study, we evaluated the chemical composition of the phloem and xylem saps of C. roseus to infer the nutritional requirements of phloem- and xylem-limited phytopathogens. Periwinkle phloem sap consisted of a rich mixture of sugars, organic acids, amino acids, amines, fatty acids, sugar acids and sugar alcohols while xylem contained similar compounds in lesser concentrations. Plant sap analysis may lead to a better understanding of the biology of fastidious Mollicutes and their complex nutritional requirements, and to successful culture of phytoplasmas and other uncultured phloem-restricted bacteria such as Candidatus Liberibacter asiaticus, the causal agent of huanglongbing in citrus.
[Mh] Termos MeSH primário: Bactérias/crescimento & desenvolvimento
Catharanthus/microbiologia
Floema/microbiologia
Xilema/microbiologia
[Mh] Termos MeSH secundário: Aminas/análise
Aminoácidos/análise
Bactérias/classificação
Produtos Biológicos/química
Produtos Biológicos/metabolismo
Carboidratos/análise
Catharanthus/metabolismo
Ácidos Graxos/análise
Cromatografia Gasosa-Espectrometria de Massas
Interações Hospedeiro-Patógeno
Concentração de Íons de Hidrogênio
Floema/metabolismo
Phytoplasma/fisiologia
Doenças das Plantas/microbiologia
Álcoois Açúcares/análise
Tenericutes/fisiologia
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Amino Acids); 0 (Biological Products); 0 (Carbohydrates); 0 (Fatty Acids); 0 (Sugar Alcohols)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


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[PMID]:27550200
[Au] Autor:Kuriakose GC; Palem PP; Jayabaskaran C
[Ad] Endereço:Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India.
[Ti] Título:Fungal vincristine from Eutypella spp - CrP14 isolated from Catharanthus roseus induces apoptosis in human squamous carcinoma cell line -A431.
[So] Source:BMC Complement Altern Med;16(1):302, 2016 Aug 22.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp - CrP14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in vitro against HeLa cell line. The present study was conducted to identify the anticancer compound responsible for the anti-proliferative activity of the fungal extract and to evaluate its in vitro anticancer and apoptotic effects. METHODS: The anti-proliferative activity of the fungal anticancer compound, vincristine was analyzed by MTT assay against different cancer cell lines. We examined its efficacy of apoptotic induction on A431 cells. The parameters examined included cell cycle distribution, loss of mitochondrial membrane potential (MMP), DNA fragmentation and reactive oxygen species (ROS) generation. RESULTS: The presence of vincristine in fungal culture filtrate was confirmed through chromatographic and spectroscopic analyses, and the amount was estimated to be 53 ± 5.0 µg/l. The partially purified fungal vincristine had strong cytotoxic activity towards human squamous carcinoma cells - A431 in the MTT assay. Furthermore, we showed that the fungal vincristine was capable of inducing apoptosis in A431 cells through generation of reactive oxygen species and activation of the intrinsic pathway leading to loss of MMP. CONCLUSIONS: We have demonstrated for the first time that the vincristine from Eutypella spp - CrP14 is an efficient inducer of apoptosis in A431 cells, meriting its further evaluation in vivo.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
Catharanthus/microbiologia
Vincristina/farmacologia
Xylariales/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Carcinoma de Células Escamosas
Linhagem Celular Tumoral
Seres Humanos
Vincristina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 5J49Q6B70F (Vincristine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1299-2


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[PMID]:27534099
[Au] Autor:Wang M; Zi J; Zhu J; Chen S; Wang P; Song L; Yu R
[Ti] Título:Artemisinic Acid Serves as a Novel ORCA3 Inducer to Enhance Biosynthesis of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells.
[So] Source:Nat Prod Commun;11(6):715-7, 2016 Jun.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the effect of artemisinic acid (AA) on improving the production of terpenoid indole alkaloids (TIAs) of Catharanthus roseus cambial meristematic cells (CMCs), feeding AA to C. roseus CMCs caused 2.35-fold and 2.51-fold increases in the production of vindoline and catharanthine, respectively, compared with those of the untreated CMCs. qRT-PCR experiments showed that AA resulted in a 1.36-8.52 fold increase in the transcript levels of several related genes, including octadecanoid-derivative responsive Catharanthus AP2-domain protein 3 (ORCA3), tryptophan decarboxylase (TDC), strictosidine synthase (STR) and desacetoxyvindoline 4-hydroxylase (D4H). However, no effect was observed on the concentration of either jasmonic acid (JA), or the octadecanoid-pathway inhibitors block TIA accumulation caused by AA. The results indicated that AA might serve as a novel ORCA3 inducer to manipulate biosynthesis of TIAs in C. roseus CMCs via an unknown mechanism.
[Mh] Termos MeSH primário: Artemisininas/farmacologia
Catharanthus/metabolismo
Meristema/metabolismo
Proteínas de Plantas/genética
Alcaloides de Triptamina e Secologanina/metabolismo
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Catharanthus/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Meristema/efeitos dos fármacos
Proteínas de Plantas/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Artemisinins); 0 (ORCA3 protein, plant); 0 (Plant Proteins); 0 (Secologanin Tryptamine Alkaloids); 0 (Transcription Factors); 53N99527G7 (artemisic acid)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE



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