Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.456.500.336 [Categoria DeCS]
Referências encontradas : 28 [refinar]
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  1 / 28 MEDLINE  
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[PMID]:25765093
[Au] Autor:Tian J; Wang YG; Ma J; Yang JB; Zhou L; Ji TF; Wang AG; Su YL
[Ad] Endereço:a State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Material Medica , Beijing 100050 , China.
[Ti] Título:Hepatoprotective benzofurans and furanolignans from Gymnema tingens.
[So] Source:J Asian Nat Prod Res;17(3):268-73, 2015.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new benzofurans, gymnefuranols A (1) and B (2), together with six known furanolignans (3-8), were isolated from Gymnema tingens. The structures of the new compounds were elucidated by comprehensive analysis of the NMR and HR-MS data. Compounds 1, 2, 6, and 7 showed hepatoprotective activities against D-galactosamine-induced HL-7702 cell damage.
[Mh] Termos MeSH primário: Benzofuranos/isolamento & purificação
Benzofuranos/farmacologia
Gymnema/química
Lignanas/isolamento & purificação
Lignanas/farmacologia
Fígado/efeitos dos fármacos
[Mh] Termos MeSH secundário: Benzofuranos/química
Galactosamina/farmacologia
Lignanas/química
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzofurans); 0 (Lignans); 0 (gymnefuranol A); 0 (gymnefuranol B); 7535-00-4 (Galactosamine)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150313
[Lr] Data última revisão:
150313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150314
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2015.1016002


  2 / 28 MEDLINE  
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[PMID]:25523465
[Au] Autor:Ahamad J; Amin S; Mir SR
[Ad] Endereço:Phytopharmaceutical Research Laboratory, Faculty of Pharmacy, Jamia Hamdard University, Hamdard Nagar, New Delhi 110 062, India.
[Ti] Título:Simultaneous Quantification of Gymnemic Acid as Gymnemagenin and Charantin as ß-Sitosterol Using Validated HPTLC Densitometric Method.
[So] Source:J Chromatogr Sci;53(7):1203-9, 2015 Aug.
[Is] ISSN:1945-239X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gymnemic acid and charantin are well-established antidiabetic phytosterols found in Gymnema sylvestre and Momordica charantia, respectively. The fact that these plants are often used together in antidiabetic poly-herbal formulations lured us to develop an HPTLC densitometric method for the simultaneous quantification of their bioactive compounds. Indirect estimation of gymnemic acid as gymnemagenin and charantin as ß-sitosterol after hydrolysis has been proposed. Aluminum-backed silica gel 60 F254 plates (20 × 10 cm) were used as stationary phase and toluene-ethyl acetate-methanol-formic acid (60 : 20 : 15 : 5, v/v) as mobile phase. Developed chromatogram was scanned at 550 nm after derivatization with modified vanillin-sulfuric acid reagent. Regression analysis of the calibration data showed an excellent linear relationship between peak area versus concentration of the analytes. Linearity was found to be in the range of 500-2,500 and 100-500 ng/band for gymnemagenin and ß-sitosterol, respectively. The suitability of the developed HPTLC method for simultaneous estimation of analytes was established by validating it as per the ICH guidelines. The limits of detection and quantification for gymnemagenin were found to be ≈60 and ≈190 ng/band, and those for ß-sitosterol ≈30 and ≈90 ng/band, respectively. The developed method was found to be linear (r(2) = 0.9987 and 0.9943), precise (relative standard deviation <1.5 and <2% for intra- and interday precision) and accurate (mean recovery ranged between 98.43-101.44 and 98.68-100.20%) for gymnemagenin and ß-sitosterol, respectively. The proposed method was also found specific and robust for quantification of both the analytes and was successfully applied to herbal drugs and in-house herbal formulation without any interference.
[Mh] Termos MeSH primário: Alcaloides/análise
Cromatografia em Camada Delgada/métodos
Densitometria/métodos
Hipoglicemiantes/análise
Saponinas/análise
Sitosteroides/análise
Triterpenos/análise
[Mh] Termos MeSH secundário: Química Farmacêutica
Gymnema/química
Limite de Detecção
Momordica/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkaloids); 0 (Hypoglycemic Agents); 0 (Saponins); 0 (Sitosterols); 0 (Triterpenes); 327O38FRK1 (gymnemic acid); 5LI01C78DD (gamma-sitosterol); U396WHT2FZ (gymnemagenin)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141220
[St] Status:MEDLINE
[do] DOI:10.1093/chromsci/bmu166


  3 / 28 MEDLINE  
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[PMID]:25053002
[Au] Autor:Srisurichan S; Puthong S; Pornpakakul S
[Ad] Endereço:Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok 10330, Thailand.
[Ti] Título:Pregnane-type steroidal glycosides from Gymnema griffithii Craib.
[So] Source:Phytochemistry;106:197-206, 2014 Oct.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eight pregnane-type steroidal glycosides substituted with ortho-acetate groups were isolated from the methanolic extract of the pericarp of Gymnema griffithii fruits, and named gymnemogriffithosides A-H. Their structures were determined by spectroscopic analysis (one and two dimensional nuclear magnetic resonance, high resolution electrospray ionization mass spectrometry and attenuated total reflectance-Fourier transformed infrared spectroscopy), while the absolute structure of the steroidal skeleton of one of these was additionally determined using Mosher's method. All compounds were evaluated for their in vitro (i) cytotoxic effects against five human tumor cell lines (BT 474, Chago, Hep-G2, KATO-III and SW620) and (ii) α-glucosidase inhibitory activity.
[Mh] Termos MeSH primário: Frutas/química
Glicosídeos/química
Gymnema/química
Pregnanos/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Glicosídeos/isolamento & purificação
Seres Humanos
Concentração Inibidora 50
Estrutura Molecular
Pregnanos/isolamento & purificação
Espectrometria de Massas por Ionização por Electrospray
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Glycoside Hydrolase Inhibitors); 0 (Glycosides); 0 (Pregnanes); 0 (pregnane glycoside)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140724
[St] Status:MEDLINE


  4 / 28 MEDLINE  
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[PMID]:23702904
[Au] Autor:Tian J; Ma QG; Yang JB; Wang AG; Ji TF; Wang YG; Su YL
[Ad] Endereço:State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Material Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
[Ti] Título:Hepatoprotective phenolic glycosides from Gymnema tingens.
[So] Source:Planta Med;79(9):761-7, 2013 Jun.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Six new phenolic diglycosides, named gymnetinosides A-F (1-6), were isolated from the ethanolic extract of Gymnema tingens, together with three known diglycosides, sequinoside K (7), khaephuoside B (8), and albibrissinoside A (9). The structures of the new compounds were determined by spectroscopic techniques including 1D-, 2D NMR, mass spectroscopy, and circular dichroism. Compounds 1, 5, and 6 showed hepatoprotective activities against D-galactosamine-induced HL-7702 cell damage.
[Mh] Termos MeSH primário: Gymnema/química
Substâncias Protetoras/química
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular/efeitos dos fármacos
Dicroísmo Circular
Avaliação Pré-Clínica de Medicamentos/métodos
Galactosamina/toxicidade
Glicosídeos/química
Glicosídeos/farmacologia
Seres Humanos
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Fenóis/química
Extratos Vegetais/análise
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycosides); 0 (Phenols); 0 (Plant Extracts); 0 (Protective Agents); 7535-00-4 (Galactosamine)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:130618
[Lr] Data última revisão:
130618
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130525
[St] Status:MEDLINE
[do] DOI:10.1055/s-0032-1328587


  5 / 28 MEDLINE  
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[PMID]:23692085
[Au] Autor:Ramkumar KM; Manjula C; Elango B; Krishnamurthi K; Saravana Devi S; Rajaguru P
[Ad] Endereço:SRM Research Institute, SRM University, Kattankulathur, Tamil Nadu 603 203, India. ramkumar.km@res.srmuniv.ac.in
[Ti] Título:In vitro cytotoxicity of Gymnema montanum in human leukaemia HL-60 cells; induction of apoptosis by mitochondrial membrane potential collapse.
[So] Source:Cell Prolif;46(3):263-71, 2013 Jun.
[Is] ISSN:1365-2184
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Gymnema montanum Hook, an Indian Ayurvedic medicinal plant, is used traditionally to treat a variety of ailments. Here, we report anti-cancer effects and molecular mechanisms of ethanolic extract of G. montanum (GLEt) on human leukaemia HL-60 cells, compared to peripheral blood mononuclear cells. MATERIALS AND METHODS: HL-60 cells were treated with different concentrations of GLEt (10-50 µg/ml) and cytotoxicity was assessed by MTT assay. Levels of lipid peroxidation, antioxidants, mitochondrial membrane potential and caspase-3 were measured. Further, apoptosis was studied using annexin-V staining and the cell cycle was analyzed by flow cytometry. RESULTS: GLEt had a potent cytotoxic effect on HL-60 cells (IC50 -20 µg/ml), yet was not toxic to normal peripheral blood mononuclear cells. Exposure of HL-60 cells to GLEt led to elevated levels of malonaldehyde formation, but to reduced glutathione, superoxide dismutase, catalase and glutathione peroxidase activities (P < 0.05). Induction of apoptosis was confirmed by observing annexin-V positive cells, associated with loss of mitochondrial membrane potential. Cell cycle arrest at G0/G1 was observed in GLEt-treated HL-60 cells, indicating its potential at inducing their apoptosis. CONCLUSIONS: Findings of the present study suggest that G. montanum induced apoptosis in the human leukaemic cancer cells, mediated by collapse of mitochondrial membrane potential, generation of reactive oxygen species and depletion of intracellular antioxidant potential.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Gymnema
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anexina A5/metabolismo
Antioxidantes/metabolismo
Caspase 3/metabolismo
Linhagem Celular Tumoral
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Células HL-60
Seres Humanos
Leucemia/tratamento farmacológico
Leucócitos Mononucleares/efeitos dos fármacos
Peroxidação de Lipídeos/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Annexin A5); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Plant Extracts); 0 (Reactive Oxygen Species); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:130522
[Lr] Data última revisão:
130522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130523
[St] Status:MEDLINE
[do] DOI:10.1111/cpr.12033


  6 / 28 MEDLINE  
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[PMID]:22432729
[Au] Autor:Ulbricht C; Abrams TR; Basch E; Davies-Heerema T; Foppa I; Hammerness P; Rusie E; Tanguay-Colucci S; Taylor S; Ulbricht C; Varghese M; Weissner W; Woods J
[Ad] Endereço:Massachusetts General Hospital, Boston, Massachusetts, USA. ulbricht@naturalstandard.com
[Ti] Título:An evidence-based systematic review of gymnema (Gymnema sylvestre R. Br.) by the Natural Standard Research Collaboration.
[So] Source:J Diet Suppl;8(3):311-30, 2011 Sep.
[Is] ISSN:1939-022X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An evidence-based systematic review of gymnema (Gymnema sylvestre R. Br.), including written and statistical analysis of scientific literature, expert opinion, folkloric precedent, history, pharmacology, kinetics/dynamics, interactions, adverse effects, toxicology, and dosing.
[Mh] Termos MeSH primário: Gymnema
Fitoterapia
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Gymnema/efeitos adversos
Seres Humanos
Medicina Tradicional
Extratos Vegetais/efeitos adversos
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:120321
[Lr] Data última revisão:
120321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120322
[St] Status:MEDLINE
[do] DOI:10.3109/19390211.2011.597977


  7 / 28 MEDLINE  
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[PMID]:21978819
[Au] Autor:Ramkumar KM; Vanitha P; Uma C; Suganya N; Bhakkiyalakshmi E; Sujatha J
[Ad] Endereço:Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, Tamilnadu, India. ramkumar@ktr.srmuniv.ac.in
[Ti] Título:Antidiabetic activity of alcoholic stem extract of Gymnema montanum in streptozotocin-induced diabetic rats.
[So] Source:Food Chem Toxicol;49(12):3390-4, 2011 Dec.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, the effect of alcoholic stem extract of Gymnema montanum (GMSt) on blood glucose, plasma insulin, and carbohydrate metabolic enzymes were studied in experimental diabetes. Diabetes mellitus was induced by a single intraperitoneal injection of STZ (60 mg/kg bw). Five days after STZ induction, diabetic rats received GMSt orally at the doses of 25, 50, 100 and 200mg/kg daily for 3 weeks. Graded doses of stem extract showed a significant reduction in blood glucose levels and improvement in plasma insulin levels. The effect was more pronounced in 100 and 200mg/kg than 50mg/kg. GMSt showed significant increase in hexokinase, Glucose-6-phosphate dehydrogenase and glycogen content in liver of diabetic rats while there was significant reduction in the levels of glucose-6-phosphatase and fructose-1,6-bisphosphatase. The present study clearly indicated significant antidiabetic effect with the stem extract of G. montanum and lends support for its traditional usage.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/tratamento farmacológico
Gymnema/química
Hipoglicemiantes/administração & dosagem
Fitoterapia
Extratos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Glicemia/efeitos dos fármacos
Diabetes Mellitus Experimental/patologia
Relação Dose-Resposta a Droga
Frutose-Bifosfatase/análise
Frutose-Bifosfatase/efeitos dos fármacos
Frutose-Bifosfatase/metabolismo
Glucose-6-Fosfatase/análise
Glucose-6-Fosfatase/efeitos dos fármacos
Glucose-6-Fosfatase/metabolismo
Glucosefosfato Desidrogenase/análise
Glucosefosfato Desidrogenase/efeitos dos fármacos
Glucosefosfato Desidrogenase/metabolismo
Glicogênio/análise
Glicogênio/metabolismo
Insulina/sangue
Fígado/efeitos dos fármacos
Fígado/enzimologia
Masculino
Folhas de Planta/química
Caules de Planta/química
Plantas Medicinais
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Plant Extracts); 9005-79-2 (Glycogen); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); EC 3.1.3.11 (Fructose-Bisphosphatase); EC 3.1.3.9 (Glucose-6-Phosphatase)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:111123
[Lr] Data última revisão:
111123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111008
[St] Status:MEDLINE
[do] DOI:10.1016/j.fct.2011.09.027


  8 / 28 MEDLINE  
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[PMID]:21913493
[Au] Autor:Haque N; Salma U; Nurunnabi TR; Uddin MJ; Jahangir MF; Islam SM; Kamruzzaman M
[Ti] Título:Management of type 2 diabetes mellitus by lifestyle, diet and medicinal plants.
[So] Source:Pak J Biol Sci;14(1):13-24, 2011 Jan 01.
[Is] ISSN:1028-8880
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:Globally, the prevalence of chronic, noncommunicable diseases is increasing at an alarming rate and diabetes is one of them. If diabetes is not controlled then a lot of complication like coronary artery disease, cerebrovascular disease, peripheral vascular disease, retinopathy, nephropathy and neuropathy arise in diabetic patients and causes morbidity and/or mortality. Diabetes is increasing at an epidemic form and in near future the largest increases will take place in the regions dominated by developing economies. So, it will be a great social and economical burden to developing countries as well as the developed. But if we be aware about our diet and lifestyle and take proper medication we may prevent and reduce the prevalence of diabetes. Oral medicine plays an important role in management of diabetes. But most of the oral drugs are costly and have a lot of side effects. For this it is also necessary to take medicines with fewer or no side effects. And antidiabetic medicinal plants may play an important role in this case. In this article we have tried to describe how diet and lifestyle with using medicinal plants may help to prevent or maintain diabetes and help to reduce the mortality and morbidity due to diabetes or complication related to it.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/terapia
Extratos Vegetais/farmacologia
Plantas Medicinais/metabolismo
[Mh] Termos MeSH secundário: Administração Oral
Glicemia/metabolismo
Cucurbitaceae
Diabetes Mellitus Tipo 2/tratamento farmacológico
Diabetes Mellitus Tipo 2/metabolismo
Dieta
Alho
Índice Glicêmico
Gymnema
Seres Humanos
Estilo de Vida
Ocimum basilicum
Cebolas
Resultado do Tratamento
Trigonella
Vinca
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Plant Extracts)
[Em] Mês de entrada:1110
[Cu] Atualização por classe:150311
[Lr] Data última revisão:
150311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110915
[St] Status:MEDLINE


  9 / 28 MEDLINE  
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[PMID]:19950392
[Au] Autor:Ramkumar KM; Sankar L; Manjula C; Krishnamurthi K; Devi SS; Chakrabarti T; Kalaiselvi K; Palanivel M; Rajaguru P
[Ad] Endereço:Department of Biotechnology, Anna University Tiruchirappalli, Tiruchirappalli 620 024, Tamil Nadu, India.
[Ti] Título:Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line.
[So] Source:Environ Mol Mutagen;51(4):285-93, 2010 May.
[Is] ISSN:1098-2280
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study we have evaluated the genoprotective effect of the ethanol extract of Gymnema montanum (GLEt) leaves in human peripheral blood lymphocytes and HL-60 cell line in vitro using the comet assay. DNA damage was induced by treating the cells with H(2)O(2) and methyl methane sulphonate (MMS). GLEt treatment effectively protected the lymphocytes and HL-60 cell line from H(2)O(2)-induced oxidative DNA damage in a dose-dependent manner whereas it was not effective against alkylative DNA damage caused by MMS. The global percent repair efficiency also showed that both pre- and post- GLEt treatment provided effective protection against H(2)O(2) induced DNA damage but not as effective against MMS. At 200 microg ml(-1) level, its repair capacity against H(2)O(2) induced DNA damage was comparable to that of vitamin-C (100 microM). Furthermore, exposure to GLEt reduced the formation of apoptotic cells caused by H(2)O(2), which was demonstrated by the decreased sub-G1-DNA content in cell cycle analysis and apoptotic frequencies of lymphocytes in an annexin-V binding assay. In addition, GLEt was found to have effective peroxide scavenging ability in dose-dependent manner. The protective efficiency of the extract was found to be directly proportional to its total phenolic content. The present study indicates that G. montanum leaves are a significant source of phytochemicals with antigenotoxic and antioxidant activity, and thus has potential therapeutic use.
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Dano ao DNA/efeitos dos fármacos
Gymnema/química
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Células HL-60
Seres Humanos
Hidroxibenzoatos/análise
Linfócitos/efeitos dos fármacos
Linfócitos/metabolismo
Mutagênese/efeitos dos fármacos
Extratos Vegetais/farmacologia
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimutagenic Agents); 0 (Antioxidants); 0 (Hydroxybenzoates); 0 (Plant Extracts); 0 (Protective Agents); 29656-58-4 (phenolic acid)
[Em] Mês de entrada:1004
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091202
[St] Status:MEDLINE
[do] DOI:10.1002/em.20543


  10 / 28 MEDLINE  
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[PMID]:19910683
[Au] Autor:Ramkumar KM; Lee AS; Krishnamurthi K; Devi SS; Chakrabarti T; Kang KP; Lee S; Kim W; Park SK; Lee NH; Rajaguru P
[Ad] Endereço:Department of Internal Medicine, Research Institute of Clinical Medicine and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, South Korea.
[Ti] Título:Gymnema montanum H. protects against alloxan-induced oxidative stress and apoptosis in pancreatic beta-cells.
[So] Source:Cell Physiol Biochem;24(5-6):429-40, 2009.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study evaluated the molecular mechanism of antidiabetic property of G. montanum leaf extract (GLEt) against alloxan-induced apoptotic cell death in rat insulinoma cells (RINm5F). The pre-treatment of GLEt (5 microg and 10 microg/ml) resulted in significant decrease in intracellular Ca(2+) concentration, nitric oxide (NO) production along with increase in mitochondrial membrane potential in alloxan (7mM/ml) treated cells. Further GLEt reduced apoptosis by inhibiting the release of cytochrome c and subsequent cleavage of PARP and caspase-3. The immunochemical staining of 8-hydroxydeoxyguanosine (8-OHdG) also evidenced the suppression of oxidative stress by GLEt. The cell cycle analysis, annexin-V labelling assay and TUNEL assay showed the suppression of apoptosis by the treatment of GLEt. Moreover, GLEt significantly increased the cellular antioxidant levels and decreased the lipid peroxides in alloxan-treated RINm5F cells. Taken together, these findings suggest that G. montanum protects pancreatic beta-cells against reactive oxygen species (ROS) by counteracting with mitochondrial membrane permeability and inhibition of the apoptotic pathway.
[Mh] Termos MeSH primário: Aloxano/toxicidade
Apoptose
Gymnema/química
Células Secretoras de Insulina/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Cálcio/metabolismo
Caspase 3/metabolismo
Células Secretoras de Insulina/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Óxido Nítrico/metabolismo
Estresse Oxidativo
Oxirredutases/metabolismo
Extratos Vegetais/química
Folhas de Planta/química
Ratos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 31C4KY9ESH (Nitric Oxide); 6SW5YHA5NG (Alloxan); EC 1.- (Oxidoreductases); EC 3.4.22.- (Caspase 3); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1002
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091114
[St] Status:MEDLINE
[do] DOI:10.1159/000257480



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