Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.456.750.391 [Categoria DeCS]
Referências encontradas : 273 [refinar]
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  1 / 273 MEDLINE  
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[PMID]:27774729
[Au] Autor:Rho T; Jung M; Lee MW; Chin YW; Yoon KD
[Ad] Endereço:College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon, Republic of Korea.
[Ti] Título:Efficient methods for isolating five phytochemicals from Gentiana macrophylla using high-performance countercurrent chromatography.
[So] Source:J Sep Sci;39(24):4723-4731, 2016 Dec.
[Is] ISSN:1615-9314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Efficient high-performance countercurrent chromatography methods were developed to isolate five typical compounds from the extracts of Gentiana macrophylla. n-Butanol-soluble extract of G. macrophylla contained three hydrophilic iridoids, loganic acid (1), swertiamarin (2) and gentiopicroside (3), and a chromene derivative, macrophylloside D (4) which were successfully isolated by flow rate gradient (1.5 mL/min in 0-60 min, 5.0 mL/min in 60-120 min), and consecutive flow rate gradient HPCCC using n-butanol/0.1% aqueous trifluoroacetic acid (1:1, v/v, normal phase mode) system. The yields of 1-4 were 22, 16, 122, and 6 mg, respectively, with purities over 97% in a flow rate gradient high-performance countercurrent chromatography, and consecutive flow rate gradient high-performance countercurrent chromatography gave 1, 2, 3 (54, 41, 348 mg, respectively, purities over 97%) and 4 (13 mg, purity at 95%) from 750 mg of sample. The main compound in methylene chloride soluble extract, 2-methoxyanofinic acid, was successfully separated by n-hexane/ethyl acetate/methanol/water (4:6:4:6, v/v/v/v, flow-rate: 4 mL/min, reversed phase mode) condition. The structures of five isolates were elucidated by H, C NMR and ESI-Q-TOF-MS spectroscopic data which were compared with previously reported values.
[Mh] Termos MeSH primário: Gentiana/química
Compostos Fitoquímicos/isolamento & purificação
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Distribuição Contracorrente
Hexanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hexanes); 0 (Phytochemicals); 0 (Plant Extracts); 2DDG612ED8 (n-hexane)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/jssc.201600988


  2 / 273 MEDLINE  
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[PMID]:28624556
[Au] Autor:Shimada A; Okumura A; Yamasaki S; Iwata Y; Koizumi N; Nishihara M; Mishiba KI
[Ad] Endereço:Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen, Nakaku, Sakai, Osaka 599-8531, Japan.
[Ti] Título:A 64-bp sequence containing the GAAGA motif is essential for CaMV-35S promoter methylation in gentian.
[So] Source:Biochim Biophys Acta;1860(8):861-869, 2017 08.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study investigated sequence specificity and perenniality of DNA methylation in the cauliflower mosaic virus (CaMV) 35S promoter of transgenic gentian (Gentiana triflora×G. scabra) plants. Unlike conventional transgene silencing models, 35S promoter hypermethylation in gentian is species-specific and occurs irrespective of the T-DNA copy number and genomic location. Modified 35S promoters were introduced into gentian, and single-copy transgenic lines were selected for methylation analysis. Modified 35S promoter lacking a core (-90) region [35S(Δcore)] in gentian conferred hypermethylation and high levels of de novo methylation of the CpHpH/CpCpG sites in the 35S enhancer regions (-298 to -241 and -148 to -85). Therefore, promoter transcription may not be an absolute requirement for the methylation machinery. In vitro, de novo methylation persisted for more than eight years. In another modified 35S promoter, two "GAAGA" motifs (-268 to -264 and -135 to -131) were replaced by "GTTCA" in the two highly de novo methylated regions. It did not support hypermethylation and showed transgene expression. A 64-bp fragment of the 35S enhancer region (-148 to -85) was introduced into gentian and the resultant transgenic lines analyzed. The 64-bp region exhibited hypermethylation at the CpG/CpWpG sites, but the CpHpH/CpCpG methylation frequency was lower than those of the unmodified 35S- and 35S(Δcore) promoters. Nevertheless, a distinct CpHpH/CpCpG methylation peak was found in the 64-bp region of all single-copy transgenic lines. These results suggest that the 64-bp region may contain an element required for 35S methylation but insufficient for high de novo methylation compared with those in the unmodified 35S and 35S(Δcore) promoters.
[Mh] Termos MeSH primário: Caulimovirus/genética
Metilação de DNA/genética
Gentiana/genética
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Regulação da Expressão Gênica de Plantas/genética
Inativação Gênica/fisiologia
Plantas Geneticamente Modificadas/genética
Transcrição Genética/genética
Transgenes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (T-DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE


  3 / 273 MEDLINE  
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[PMID]:28129760
[Au] Autor:Mubashir K; Ganai BA; Ghazanfar K; Akbar S; Rah B; Tantry M; Masood A
[Ad] Endereço:School of Medicine, Goba Referral Hospital, Madda Walabu University, Bale Robe, Ethiopia. kmubashir216@gmail.com.
[Ti] Título:Anti-inflammatory and immuno-modulatory studies on LC-MS characterised methanol extract of Gentiana kurroo Royle.
[So] Source:BMC Complement Altern Med;17(1):78, 2017 Jan 28.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In ayurvedic traditional medicine Gentiana kurroo Royle (family; Gentianaceae) is used to treat several metabolic diseases. This plant is rich in various compounds belonging to flavonoids and glycosides. Till now little work has been carried out on immunomodulatory and anti-inflammatory potential of this plant. This study confirms the presence of bioactive compounds and evaluates the anti-inflammatory and immunomodulatory effect of this plant. METHODS: To carry out this work, the methanol extract was investigated in different doses using in vivo and in vitro models. In vivo study involved haemagglutination titre and DTH methods, and in vitro study was done using splenocyte proliferation assay and LPS stimulated macrophage culture. TNF-α, IL-6 and NO were assayed using ELISA kit methods, while NF-κB was evaluated by western blotting. LC-ESI-MS/MS was used for the characterization of the methanol extract. RESULTS: The results showed suppression of both humoral and cell mediated immunity in vivo. This effect was also observed by inhibition of B and T cell proliferation in splenocyte proliferation assay. TNF-α, IL-6 and NO concentrations were also less in extract treated macrophage cultures. The NF-κB expression was also lowered in treated macrophages as compared to untreated macrophages. All these observations were found to be dose dependent. LC-MS characterization of this extract showed the presence of known compounds which are glycosides, alkaloids and flavonoids in nature. CONCLUSION: The methanol extract of this plant was found to be rich in glycoside, alkaloid and flavonoid compounds. These compounds are probably responsible for the suppression of immune response and anti-inflammatory activity. The extract as such and identified bioactive compounds can be useful for the treatment of inflammatory disorders.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Gentiana/química
Fatores Imunológicos/farmacologia
Mediadores da Inflamação/metabolismo
Inflamação
Linfócitos/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/análise
Alcaloides/farmacologia
Alcaloides/uso terapêutico
Animais
Anti-Inflamatórios/análise
Anti-Inflamatórios/uso terapêutico
Linhagem Celular
Citocinas/metabolismo
Flavonoides/análise
Flavonoides/farmacologia
Flavonoides/uso terapêutico
Glicosídeos/análise
Glicosídeos/farmacologia
Glicosídeos/uso terapêutico
Hipersensibilidade Tardia/induzido quimicamente
Hipersensibilidade Tardia/tratamento farmacológico
Hipersensibilidade Tardia/metabolismo
Imunidade/efeitos dos fármacos
Fatores Imunológicos/análise
Fatores Imunológicos/uso terapêutico
Inflamação/induzido quimicamente
Inflamação/tratamento farmacológico
Inflamação/metabolismo
Macrófagos/metabolismo
Masculino
Medicina Ayurvédica
Camundongos Endogâmicos BALB C
NF-kappa B/metabolismo
Óxido Nítrico/metabolismo
Fitoterapia
Extratos Vegetais/química
Extratos Vegetais/uso terapêutico
Raízes de Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Flavonoids); 0 (Glycosides); 0 (Immunologic Factors); 0 (Inflammation Mediators); 0 (NF-kappa B); 0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1593-7


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[PMID]:28078812
[Au] Autor:Wang K; Coyle ME; Mansu S; Zhang AL; Xue CC
[Ad] Endereço:China-Australia International Research Centre for Chinese Medicine (CAIRCCM), School of Health and Biomedical Sciences, RMIT University, Bundoora Campus, Melbourne, VIC, 3083, Australia.
[Ti] Título:Gentiana scabra Bunge. Formula for Herpes Zoster: Biological Actions of Key Herbs and Systematic Review of Efficacy and Safety.
[So] Source:Phytother Res;31(3):375-386, 2017 Mar.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reviewed the biological action of key herbs and evaluated systematically the efficacy and safety of oral Gentiana formula for herpes zoster (HZ). Experimental studies relevant to HZ were identified in PubMed. Randomized controlled trials using Gentiana formula for HZ were identified from nine English and Chinese databases. The primary outcome was evaluation of pain. Potential risk of bias was assessed. Meta-analysis was conducted using mean difference or risk ratio with 95% confidence intervals. Key herbs Gentiana scabra Bunge, Gentiana triflora Pall, Scutellaria baicalensis Georgi, and Gardenia jasminoides Ellis have shown antiinflammatory actions through inhibition of inflammatory cytokines and pro-inflammatory enzymes. Twenty-six clinical studies, involving 2955 participants, were included. Modified Gentiana formula resolved pain earlier than pharmacotherapy when used alone or combined with topical Chinese herbal medicine. Incidence of postherpetic neuralgia was lower (risk ratio 0.14, 95% confidence interval 0.03 to 0.74) with modified Gentiana formula plus topical Chinese herbal medicine. Mild adverse events were reported. Antiinflammatory actions of key herbs of Gentiana formula may explain clinical benefit in hastening pain relief and decreasing postherpetic neuralgia. Few adverse events were reported. Findings were limited by study quality and diversity in intervention and comparator dosage. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Gentiana/química
Herpes Zoster/tratamento farmacológico
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/uso terapêutico
Asteraceae/química
Seres Humanos
Neuralgia Pós-Herpética/tratamento farmacológico
Fitoterapia/efeitos adversos
Extratos Vegetais/efeitos adversos
Extratos Vegetais/farmacologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 0 (Scutellaria baicalensis extract)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5769


  5 / 273 MEDLINE  
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[PMID]:28060552
[Au] Autor:Sheu MJ; Chiu CC; Yang DJ; Hsu TC; Tzang BS
[Ad] Endereço:1 Division of Gastroenterology and Hepatology, Chi Mei Medical Center , Tainan, Taiwan .
[Ti] Título:The Root Extract of Gentiana macrophylla Pall. Alleviates B19-NS1-Exacerbated Liver Injuries in NZB/W F1 Mice.
[So] Source:J Med Food;20(1):56-64, 2017 Jan.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nonstructural protein NS1 of human parvovirus B19 (B19) is known to exacerbate disease activity in systemic lupus erythematosus (SLE). However, no specific medicine for B19 infection is available. The roots of Gentiana macrophylla Pall. (GM), the traditional Chinese medicine "Qinjiao," have been used for centuries to treat rheumatic disease, including SLE. Herein, we aimed to investigate the effects of GM root extract (100 and 300 mg/kg body weight) on B19-NS1-exacerbated liver injury in NZB/W F1 mice; liver tissues were assessed by hematoxylin-eosin staining and immunoblotting. The GM root extract significantly decreased B19-NS1-exacerbated liver inflammation by suppressing the expressions of hepatic inducible nitric oxide synthase, cyclooxygenase type 2 (COX-2), interleukin (IL)-1ß proteins, values of serum asparate transaminase (AST) and alanine transaminase (ALT), and lymphocyte infiltration (P < .05). It also significantly reduced the B19-NS1-exacerbated hepatic matrix metalloproteinase-9 (MMP-9) and urokinase plasminogen activator (uPA) expressions by downregulating tumor necrosis factor (TNF)-α/NF-κB (p65) signaling. These findings suggest a therapeutic potential of GM root extract against B19-NS1-exacerbated liver inflammation in SLE.
[Mh] Termos MeSH primário: Gentiana/química
Hepatopatias/tratamento farmacológico
Hepatopatias/virologia
Parvovirus B19 Humano/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Ciclo-Oxigenase 2/genética
Ciclo-Oxigenase 2/imunologia
Feminino
Seres Humanos
Interleucina-1beta/genética
Interleucina-1beta/imunologia
Fígado/efeitos dos fármacos
Fígado/imunologia
Hepatopatias/genética
Hepatopatias/imunologia
Metaloproteinase 9 da Matriz/genética
Metaloproteinase 9 da Matriz/imunologia
Camundongos
Camundongos Endogâmicos NZB
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/imunologia
Infecções por Parvoviridae/tratamento farmacológico
Infecções por Parvoviridae/genética
Infecções por Parvoviridae/imunologia
Infecções por Parvoviridae/virologia
Parvovirus B19 Humano/fisiologia
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-1beta); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.3817


  6 / 273 MEDLINE  
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[PMID]:27422574
[Au] Autor:Ni L; Zhao Z; Xu H; Chen S; Dorje G
[Ad] Endereço:School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
[Ti] Título:Chloroplast genome structures in Gentiana (Gentianaceae), based on three medicinal alpine plants used in Tibetan herbal medicine.
[So] Source:Curr Genet;63(2):241-252, 2017 May.
[Is] ISSN:1432-0983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genus Gentiana is the largest in the Gentianaceae family with ca. 400 species. However, with most species growing on the Qinghai-Tibet plateau, the processes of adaptive evolution and speciation within the genus is not clear. Also, the genomic analyses could provide important information. So far, the complete chloroplast (cp) genome data of the genus are still deficient. As the second and third sequenced members within Gentianaceae, we report the construction of complete cp sequences of Gentiana robusta King ex Hook. f. and Gentiana crassicaulis Duthie ex Burk., and describe a comparative study of three Gentiana cp genomes, including the cp genome of Gentiana straminea Maxim. published previously. These cp genomes are highly conserved in gene size, gene content, and gene order and the rps16 pseudogene with exon2 missing was found common. Three repeat types and five SSR types were investigated, and the number and distribution are similar among the three genomes. Sixteen genome divergent hotspot regions were identified across these cp genomes that could provide potential molecular markers for further phylogenetic studies in Gentiana. The IR/SC boundary organizations in Gentianales cp genomes were compared and three different types of boundaries were observed. Six data partitions of cp genomes in Gentianales were used for phylogenetic analyses and different data partitions were largely congruent with each other. The ML phylogenetic tree was constructed based on the fragments in cp genomes commonly available in 33 species from Lamiids, including 12 species in Gentianales, 1 in Boraginaceae, 10 in Solanales, and 10 in Lamiales. The result strongly supports the position of Boraginaceae (Ehretia acuminata) as the sister of Solanales, with the bootstrap values of 97 %. This study provides a platform for further research into the molecular phylogenetics of species in the order Gentianales (family Gentianaceae) notably in respect of speciation and species identification.
[Mh] Termos MeSH primário: DNA Circular/genética
Genoma de Cloroplastos/genética
Genômica/métodos
Gentiana/genética
Medicina Herbária
Plantas Medicinais/genética
[Mh] Termos MeSH secundário: Proteínas de Cloroplastos/genética
DNA de Cloroplastos/química
DNA de Cloroplastos/genética
DNA de Cloroplastos/isolamento & purificação
DNA Circular/química
Ordem dos Genes
Genes de Cloroplastos/genética
Gentiana/classificação
Filogenia
Plantas Medicinais/classificação
Análise de Sequência de DNA
Especificidade da Espécie
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chloroplast Proteins); 0 (DNA, Chloroplast); 0 (DNA, Circular)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160717
[St] Status:MEDLINE
[do] DOI:10.1007/s00294-016-0631-1


  7 / 273 MEDLINE  
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[PMID]:27342232
[Au] Autor:Wu S; Ning Y; Zhao Y; Sun W; Thorimbert S; Dechoux L; Sollogoub M; Zhang Y
[Ti] Título:Research Progress of Natural Product Gentiopicroside - a Secoiridoid Compound.
[So] Source:Mini Rev Med Chem;17(1):62-77, 2017.
[Is] ISSN:1875-5607
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gentiopicroside is a secoiridoid compound isolated from Gentiana lutea which is called Qin Jiao in Chinese. It is one of the most common herbal medicines used in China. In this article, we review the pharmacological and biological activity (antiviral, anti-inflammatory, analgesia, antihepatotoxic and choleretic), as well as biotransformation of the gentiopicroside. In addition, attempt towards the total synthesis of gentiopicroside is also presented.
[Mh] Termos MeSH primário: Produtos Biológicos/farmacologia
Glucosídeos Iridoides/farmacologia
[Mh] Termos MeSH secundário: Analgésicos/química
Analgésicos/isolamento & purificação
Analgésicos/farmacologia
Animais
Anti-Inflamatórios não Esteroides/química
Anti-Inflamatórios não Esteroides/isolamento & purificação
Anti-Inflamatórios não Esteroides/farmacologia
Antivirais/química
Antivirais/isolamento & purificação
Antivirais/farmacologia
Produtos Biológicos/química
Produtos Biológicos/isolamento & purificação
Biotransformação
Colagogos e Coleréticos/química
Colagogos e Coleréticos/isolamento & purificação
Colagogos e Coleréticos/farmacologia
Gentiana/química
Seres Humanos
Glucosídeos Iridoides/química
Glucosídeos Iridoides/isolamento & purificação
Estrutura Molecular
Substâncias Protetoras/química
Substâncias Protetoras/isolamento & purificação
Substâncias Protetoras/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antiviral Agents); 0 (Biological Products); 0 (Cholagogues and Choleretics); 0 (Iridoid Glucosides); 0 (Protective Agents); 0WE09Z21RC (gentiopicroside)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170331
[Lr] Data última revisão:
170331
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160626
[St] Status:MEDLINE


  8 / 273 MEDLINE  
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[PMID]:27851826
[Au] Autor:Cao X; Guo X; Yang X; Wang H; Hua W; He Y; Kang J; Wang Z
[Ad] Endereço:Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
[Ti] Título:Transcriptional Responses and Gentiopicroside Biosynthesis in Methyl Jasmonate-Treated Gentiana macrophylla Seedlings.
[So] Source:PLoS One;11(11):e0166493, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gentiana macrophylla, a medicinal plant with significant pharmacological properties, contains the bioactive compound gentiopicroside. Methyl jasmonate (MeJA) is an effective elicitor for enhancing the production of such compounds. However, little is known about MeJA-mediated biosynthesis of gentiopicroside. We investigated this phenomenon as well as gene expression profiles to determine the molecular mechanisms for MeJA-mediated gentiopicroside biosynthesis and regulation in G. macrophylla. Our HPLC results showed that Gentiana macrophylla seedlings exposed to MeJA had significantly higher concentrations of gentiopicroside when compared with control plants. We used RNA sequencing to compare transcriptional profiles in seedlings treated for 5 d with either 0 µmol L-1 MeJA (C) or 250 µmol L-1 MeJA (M5) and detected differentially expressed genes (DEGs). In total, 77,482 unique sequences were obtained from approximately 34 million reads. Of these, 48,466 (57.46%) sequences were annotated based on BLASTs performed against public databases. We identified 5,206 DEGs between the C and M5 samples, including genes related to the α-lenolenic acid degradation pathway, JA signaling pathway, and gentiopicroside biosynthesis. Expression of numerous enzyme genes in the glycolysis pathway was significantly up-regulated. Many genes encoding transcription factors (e.g. ERF, bHLH, MYB, and WRKY) also responded to MeJA elicitation. Rapid acceleration of the glycolysis pathway that supplies precursors for IPP biosynthesis and up-regulates the expression of enzyme genes in that IPP pathway are probably most responsible for MeJA stimulation of gentiopicroside synthesis. Our qRT-PCR results showed that the expression profiles of 12 gentiopicroside biosynthesis genes were consistent with the RNA-Seq data. These results increase our understanding about how the gentiopicroside biosynthesis pathway in G. macrophylla responds to MeJA.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Vias Biossintéticas/genética
Ciclopentanos/farmacologia
Gentiana/genética
Glucosídeos Iridoides/metabolismo
Oxilipinas/farmacologia
Plântulas/genética
Transcrição Genética/efeitos dos fármacos
[Mh] Termos MeSH secundário: Vias Biossintéticas/efeitos dos fármacos
Ciclopentanos/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Gentiana/efeitos dos fármacos
Glicólise/efeitos dos fármacos
Anotação de Sequência Molecular
Oxilipinas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Metabolismo Secundário/efeitos dos fármacos
Metabolismo Secundário/genética
Plântulas/efeitos dos fármacos
Análise de Sequência de RNA
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
Fatores de Transcrição/metabolismo
Transcriptoma
Regulação para Cima/efeitos dos fármacos
Regulação para Cima/genética
Ácido alfa-Linolênico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (Iridoid Glucosides); 0 (Oxylipins); 0 (Transcription Factors); 0RBV727H71 (alpha-Linolenic Acid); 0WE09Z21RC (gentiopicroside); 6RI5N05OWW (jasmonic acid); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166493


  9 / 273 MEDLINE  
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[PMID]:27589396
[Au] Autor:Berman J; Sheng Y; Gómez Gómez L; Veiga T; Ni X; Farré G; Capell T; Guitián J; Guitián P; Sandmann G; Christou P; Zhu C
[Ad] Endereço:Departament de Producció Vegetal i Ciència Forestal, Universitat de Lleida-Agrotecnio Center, Lleida, Spain.
[Ti] Título:Red Anthocyanins and Yellow Carotenoids Form the Color of Orange-Flower Gentian (Gentiana lutea L. var. aurantiaca).
[So] Source:PLoS One;11(9):e0162410, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flower color is an important characteristic that determines the commercial value of ornamental plants. Gentian flowers occur in a limited range of colors because this species is not widely cultivated as a cut flower. Gentiana lutea L. var. aurantiaca (abbr, aurantiaca) is characterized by its orange flowers, but the specific pigments responsible for this coloration are unknown. We therefore investigated the carotenoid and flavonoid composition of petals during flower development in the orange-flowered gentian variety of aurantiaca and the yellow-flowered variety of G. lutea L. var. lutea (abbr, lutea). We observed minor varietal differences in the concentration of carotenoids at the early and final stages, but only aurantiaca petals accumulated pelargonidin glycosides, whereas these compounds were not found in lutea petals. We cloned and sequenced the anthocyanin biosynthetic gene fragments from petals, and analyzed the expression of these genes in the petals of both varieties to determine the molecular mechanisms responsible for the differences in petal color. Comparisons of deduced amino acid sequences encoded by the isolated anthocyanin cDNA fragments indicated that chalcone synthase (CHS), chalcone isomerase (CHI), anthocyanidin synthase 1 (ANS1) and ANS2 are identical in both aurantiaca and lutea varieties whereas minor amino acid differences of the deduced flavonone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR) between both varieties were observed. The aurantiaca petals expressed substantially higher levels of transcripts representing CHS, F3H, DFR, ANS and UDP-glucose:flavonoid-3-O-glucosyltransferase genes, compared to lutea petals. Pelargonidin glycoside synthesis in aurantiaca petals therefore appears to reflect the higher steady-state levels of pelargonidin synthesis transcripts. Moreover, possible changes in the substrate specificity of DFR enzymes may represent additional mechanisms for producing red pelargonidin glycosides in petals of aurantiaca. Our report describing the exclusive accumulation of pelargonidin glycosides in aurantiaca petals may facilitate the modification of gentian flower color by the production of red anthocyanins.
[Mh] Termos MeSH primário: Antocianinas/análise
Carotenoides/análise
Cor
Flores/química
Regulação da Expressão Gênica de Plantas
Gentiana/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Gentiana/genética
Pigmentação/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Plant Proteins); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162410


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[PMID]:27565299
[Au] Autor:Cheng Z; Zhang Y; Song H; Zhou H; Zhong F; Hu H; Feng Y
[Ad] Endereço:School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China.
[Ti] Título:Extraction optimization, characterization and antioxidant activity of polysaccharide from Gentiana scabra bge.
[So] Source:Int J Biol Macromol;93(Pt A):369-380, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, optimization of smashing tissue extraction (STE), preliminary chemical characterization and antioxidant activity in vitro of crude polysaccharides (CPS) from Gentiana scabra bge (G. scabra) were investigated. The optimal extraction conditions were determined as follows: sample particle size of 80mesh, solid/liquid ratio of 1:34, extraction voltage of 157.09V and extraction time of 130.38s. Under these conditions, the extraction yield of CPS had reached 15.03±0.14% (n=3). Chemical composition analysis indicated CPS was mainly composed of mannose, rhamnose, galacturonic acid, glcose, galactose, arabinose and fucose in a molar ratio of 1.00:9.89:51.59:35.37:38.06:99.13:21.34, respectively. The average molecular weight of CPS was estimated to be 3.8×10 Da. In addition, the potential antioxidant activity of CPS extracted by STE were demonstrated by DPPH radical scavenging assay, superoxide anion radical scavenging assay, hydroxyl radical scavenging assay and ferric reducing power assay. Overall, this study provided an effective extraction technique for G. scabra polysaccharides which would be explored as a promising natural antioxidant agent applied in functional foods or medicines.
[Mh] Termos MeSH primário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Gentiana/química
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Polysaccharides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE



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