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Pesquisa : B01.650.940.800.575.912.250.456.937.876 [Categoria DeCS]
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[PMID]:28276734
[Au] Autor:Wu Y; Jin F; Wang Y; Li F; Ren Z; Wang Y
[Ad] Endereço:a Guangzhou Jinan Biomedicine Research and Development Center, Institute of Biomedicine, College of Life Science and Technology , Jinan University , Guangzhou , P.R, China.
[Ti] Título:In vitro and in vivo inhibitory effects of 6-hydroxyrubiadin on lipopolysaccharide-induced inflammation.
[So] Source:Immunopharmacol Immunotoxicol;39(3):107-116, 2017 Jun.
[Is] ISSN:1532-2513
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inflammation is a defensive response against a multitude of harmful stimuli and stress conditions such as tissue injury, and is one of the most common pathological processes of human diseases. 6-Hydroxyrubiadin, an anthraquinone isolated from Rubia cordifolia L., exhibits several bioactive properties. The aim of this study was to evaluate whether 6-hydroxyrubiadin can reduce the production of pro-inflammatory cytokines and ameliorate acute lung injury (ALI) in a mouse model. In this study, we demonstrated that 6-hydroxyrubiadin suppressed lipopolysaccharide (LPS)-induced nuclear factor-kappa B activation as well as the phosphorylation of c-Jun N-terminal kinase in RAW 264.7 macrophages. In addition, we also showed that 6-hydroxyrubiadin inhibited the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1ß and IL-6 in phorbol myristate acetate (PMA)-primed U937 and RAW 264.7 cells. Furthermore, 6-hydroxyrubiadin treatment reduced the production of these cytokines in vivo and attenuated the severity of LPS-induced ALI. Thus, these results suggested that 6-hydroxyrubiadin may be a potential therapeutic candidate for the treatment of inflammation and inflammatory diseases.
[Mh] Termos MeSH primário: Lesão Pulmonar Aguda/induzido quimicamente
Lesão Pulmonar Aguda/tratamento farmacológico
Antraquinonas/farmacologia
Lipopolissacarídeos/toxicidade
Rubia/química
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/metabolismo
Lesão Pulmonar Aguda/patologia
Animais
Antraquinonas/química
Citocinas/biossíntese
Modelos Animais de Doenças
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Inflamação/induzido quimicamente
Inflamação/tratamento farmacológico
Inflamação/metabolismo
Camundongos
Células RAW 264.7
Células U937
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Cytokines); 0 (Lipopolysaccharides)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/08923973.2017.1295053


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[PMID]:28131591
[Au] Autor:Ford L; Henderson RL; Rayner CM; Blackburn RS
[Ad] Endereço:School of Design, University of Leeds, Leeds LS2 9JT, United Kingdom; School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom.
[Ti] Título:Mild extraction methods using aqueous glucose solution for the analysis of natural dyes in textile artefacts dyed with Dyer's madder (Rubia tinctorum L.).
[So] Source:J Chromatogr A;1487:36-46, 2017 Mar 03.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Madder (Rubia tinctorum L.) has been widely used as a red dye throughout history. Acid-sensitive colorants present in madder, such as glycosides (lucidin primeveroside, ruberythric acid, galiosin) and sensitive aglycons (lucidin), are degraded in the textile back extraction process; in previous literature these sensitive molecules are either absent or present in only low concentrations due to the use of acid in typical textile back extraction processes. Anthraquinone aglycons alizarin and purpurin are usually identified in analysis following harsh back extraction methods, such those using solvent mixtures with concentrated hydrochloric acid at high temperatures. Use of softer extraction techniques potentially allows for dye components present in madder to be extracted without degradation, which can potentially provide more information about the original dye profile, which varies significantly between madder varieties, species and dyeing technique. Herein, a softer extraction method involving aqueous glucose solution was developed and compared to other back extraction techniques on wool dyed with root extract from different varieties of Rubia tinctorum. Efficiencies of the extraction methods were analysed by HPLC coupled with diode array detection. Acidic literature methods were evaluated and they generally caused hydrolysis and degradation of the dye components, with alizarin, lucidin, and purpurin being the main compounds extracted. In contrast, extraction in aqueous glucose solution provides a highly effective method for extraction of madder dyed wool and is shown to efficiently extract lucidin primeveroside and ruberythric acid without causing hydrolysis and also extract aglycons that are present due to hydrolysis during processing of the plant material. Glucose solution is a favourable extraction medium due to its ability to form extensive hydrogen bonding with glycosides present in madder, and displace them from the fibre. This new glucose method offers an efficient process that preserves these sensitive molecules and is a step-change in analysis of madder dyed textiles as it can provide further information about historical dye preparation and dyeing processes that current methods cannot. The method also efficiently extracts glycosides in artificially aged samples, making it applicable for museum textile artefacts.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Corantes/análise
Glucose/química
Rubia/química
[Mh] Termos MeSH secundário: Animais
Antraquinonas/análise
Técnicas de Química Analítica/normas
Cromatografia Líquida de Alta Pressão
Corantes/química
Glicosídeos/química
Hidrólise
Extratos Vegetais/análise
Raízes de Plantas/química
Têxteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Coloring Agents); 0 (Glycosides); 0 (Plant Extracts); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


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[PMID]:28056930
[Au] Autor:Gong XP; Sun YY; Chen W; Guo X; Guan JK; Li DY; Du G
[Ad] Endereço:Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095# Hangkong Road, Wuhan, 430030, People's Republic of China.
[Ti] Título:Anti-diarrheal and anti-inflammatory activities of aqueous extract of the aerial part of Rubia cordifolia.
[So] Source:BMC Complement Altern Med;17(1):20, 2017 Jan 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Shaanxi province, China, the aqueous extract of Rubia cordifolia's aerial part (AERCAP) is traditionally used to manage diarrhea. However, there is no scientific evidence to verify the safety and efficacy of its use. The aim of this study was to investigate the anti-diarrheal and anti-inflammatory effects of AERCAP by using a rodent model. METHODS: The anti-diarrheal effects were studied by senna leaf-induced diarrheal and intestinal transit experiments in mice. The anti-inflammatory activity was investigated by trinitrobenzenesulfonic acid (TNBS)-induced colonic inflammation in rats. RESULTS: The results indicated that AERCAP delayed the onset of semi-solid feces, reduced the evacuation index (EI) in senna leaf-induced diarrheal in mice, and inhibited the propulsive movement in castor oil-induced intestinal transit but not in the normal intestinal transit test. The results were compared with the standard anti-diarrheal drug loperamide. Additionally, oral treatment with AERCAP significantly decreased the macroscopic damage area, improved the microscopic structure, and reduced the malondialdehyde (MDA) content, IL-1ß and TNF-α levels in colonic tissue compared with the TNBS control group in rats. CONCLUSIONS: AERCAP exhibited anti-diarrheal and anti-inflammatory activities in a rodent model. The study validated the traditional use of the plant in Chinese herbal medicine as a valuable natural remedy for the treatment of diarrhea.
[Mh] Termos MeSH primário: Anti-Inflamatórios/administração & dosagem
Antidiarreicos/administração & dosagem
Diarreia/tratamento farmacológico
Medicamentos de Ervas Chinesas/administração & dosagem
Rubia/química
[Mh] Termos MeSH secundário: Animais
Colo/efeitos dos fármacos
Colo/imunologia
Diarreia/genética
Diarreia/imunologia
Seres Humanos
Interleucina-1beta/genética
Interleucina-1beta/imunologia
Masculino
Camundongos
Folhas de Planta/química
Ratos
Ratos Wistar
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antidiarrheals); 0 (Drugs, Chinese Herbal); 0 (Interleukin-1beta); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1527-9


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[PMID]:27999402
[Au] Autor:Shan M; Yu S; Yan H; Chen P; Zhang L; Ding A
[Ad] Endereço:Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. shanmingqiu@
[Ti] Título:A Review of the Botany, Phytochemistry, Pharmacology and Toxicology of Rubiae Radix et Rhizoma.
[So] Source:Molecules;21(12), 2016 Dec 20.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Linn (Rubiaceae) is a climbing perennial herbal plant, which is widely distributed in China and India. Its root and rhizome, Rubiae Radix Rhizoma (called Qiancao in China and Indian madder in India), is a well known phytomedicine used for hematemesis, epistaxis, flooding, spotting, traumatic bleeding, amenorrhea caused by obstruction, joint impediment pain, swelling and pain caused by injuries from falls. In addition, it is a kind of pigment utilized as a food additive and a dye for wool or fiber. This review mainly concentrates on studies of the botany, phytochemistry, pharmacology and toxicology of this Traditional Chinese Medicine. The phytochemical evidences indicated that over a hundred chemical components have been found and isolated from the medicine, such as anthraquinones, naphthoquinones, triterpenoids, cyclic hexapeptides and others. These components are considered responsible for the various bioactivities of the herbal drug, including anti-oxidation, anti-inflammation, immunomodulation, antitumor, effects on coagulation-fibrinolysis system, neuroprotection and other effects. Additionally, based on these existing results, we also propose some interesting future research directions. Consequently, this review should help us to more comprehensively understand and to more fully utilize the herbal medicine Rubiae Radix Rhizoma.
[Mh] Termos MeSH primário: Extratos Vegetais/química
Extratos Vegetais/farmacologia
Extratos Vegetais/toxicidade
Rubia/química
[Mh] Termos MeSH secundário: Animais
China
Medicamentos de Ervas Chinesas/farmacologia
Seres Humanos
Camundongos
Raízes de Plantas/química
Plantas Medicinais/química
Ratos
Rizoma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


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[PMID]:27704813
[Au] Autor:Quan KT; Park HS; Oh J; Park HB; Ferreira D; Myung CS; Na M
[Ad] Endereço:Department of Pharmaceutical Analysis and Standardization, National Institute of Medicinal Materials , Hanoi, Vietnam.
[Ti] Título:Arborinane Triterpenoids from Rubia philippinensis Inhibit Proliferation and Migration of Vascular Smooth Muscle Cells Induced by the Platelet-Derived Growth Factor.
[So] Source:J Nat Prod;79(10):2559-2569, 2016 Oct 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are associated with cardiovascular diseases and related complications. Such deleterious proliferation and migration events are triggered by cytokines and growth factors, and among them, platelet-derived growth factor (PDGF) is recognized as the most potent inducer. Despite the genus Rubia being researched to identify valuable commercial and medicinal virtues, Rubia philippinensis has rarely been investigated. Nine arborinane-type triterpenoids (1-9) were identified from this underutilized plant species. In particular, 4 was identified as the first arborinane derivative carrying a ketocarbonyl motif at C-19. The presence of the cyclopentanone moiety and the associated configurational assignment were determined by utilizing NOE and coupling constant analysis. These compounds were assessed for their inhibitory potential on PDGF-induced proliferation and the migration of VSMCs. Treatment with 5 µM compound 5 (62.6 ± 10.7%) and compound 9 (41.1 ± 4.7%) impeded PDGF-stimulated proliferation without exerting cytotoxicity. Compound 7 exhibited antimigration activity in a dose-dependent manner (38.5 ± 3.0% at 10 µM, 57.6 ± 3.2% at 30 µM). These results suggest that the arborinane-type triterpenoids may be a pertinent starting point for the development of cardiovascular drugs capable of preventing the intimal accumulation of VSMCs.
[Mh] Termos MeSH primário: Músculo Liso Vascular/efeitos dos fármacos
Plantas Medicinais/química
Fator de Crescimento Derivado de Plaquetas/farmacologia
Rubia/química
Triterpenos/isolamento & purificação
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Aorta/citologia
Sobrevivência Celular/efeitos dos fármacos
Masculino
Estrutura Molecular
Músculo Liso Vascular/metabolismo
Ressonância Magnética Nuclear Biomolecular
Raízes de Plantas/química
Ratos
Triterpenos/química
Vietnã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Platelet-Derived Growth Factor); 0 (Triterpenes)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


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[PMID]:27258244
[Au] Autor:Gao M; Yang J; Wang Z; Yang B; Kuang H; Liu L; Wang L; Yang C
[Ad] Endereço:Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin 150081, Heilongjang, China. gaomingjie8888@163.com.
[Ti] Título:Simultaneous Determination of Purpurin, Munjistin and Mollugin in Rat Plasma by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry: Application to a Pharmacokinetic Study after Oral Administration of Rubia cordifolia L. Extract.
[So] Source:Molecules;21(6), 2016 Jun 01.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A specific, simple, sensitive Ultra High Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-MS/MS) method has been developed and validated for the simultaneous determination and pharmacokinetic study of purpurin, munjistin, and mollugin in rat plasma. Chromatographic separation was carried out using a C18 column (ACQUITY UPLC(®) HSS T3, 1.8 µm, 2.1 × 100 mm) with gradient elution. The compounds were detected on a 6430 triple-quadrupole tandem MS with an electrospray ionization (ESI) interface using multiple reaction monitoring (MRM) in positive ionization mode. The samples were prepared by a liquid-liquid extraction (LLE) method with ethyl acetate after being spiked with an internal standard (bifendate). The current UHPLC-MS/MS assay was validated for its linearity, intra-day and inter-day precisions, accuracy, extraction recovery, matrix effect and stability in different conditions. The method was linear for all analytes over the investigated range with all determined correlation coefficients exceeding 0.9900. The intra-day and inter-day precisions were in the range of 4.21% to 14.84%, and the relative errors of accuracies were in the range of -14.05% to 14.75%. The mean recoveries and matrix effects of purpurin, munjistin, and mollugin were higher than 78.87% and 92.56%, repectively. After oral administration of 0.82 g/kg of Rubia cordifolia extract, the maximum plasma concentrations (Cmax) were 70.10 ± 11.78 ng/mL for purpurin, 26.09 ± 6.6 ng/mL for munjistin, and 52.10 ± 6.71 ng/mL for mollugin. The time for maximal concentration (Tmax) was 1.61 ± 0.24 h for purpurin, 2.58 ± 0.19 h for munjistin, and 1.99 ± 0.21 h for mollugin. The established method was further applied to a pharmacokinetic study of purpurin, munjistin, and mollugin in rat plasma. It was concluded from the pharmacokinetic parameters that the three analytes showed a process of slow absorption and metabolism after oral administration of R. cordifolia extract to rats.
[Mh] Termos MeSH primário: Antraquinonas/farmacocinética
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacocinética
Extratos Vegetais/química
Extratos Vegetais/farmacocinética
Piranos/farmacocinética
Rubia/química
[Mh] Termos MeSH secundário: Administração Oral
Animais
Antraquinonas/química
Cromatografia Líquida de Alta Pressão
Estabilidade de Medicamentos
Medicamentos de Ervas Chinesas/administração & dosagem
Masculino
Estrutura Molecular
Extratos Vegetais/administração & dosagem
Piranos/química
Ratos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 0 (Pyrans); 55481-88-4 (rubimaillin); L1GT81LS6N (purpurin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE


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[PMID]:27251124
[Au] Autor:Shkryl YN; Veremeichik GN; Makhazen DS; Silantieva SA; Mishchenko NP; Vasileva EA; Fedoreyev SA; Bulgakov VP
[Ad] Endereço:Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Sciences, 159 Stoletija St., Vladivostok, 690022, Russia. yn80@mail.ru.
[Ti] Título:Increase of anthraquinone content in Rubia cordifolia cells transformed by native and constitutively active forms of the AtCPK1 gene.
[So] Source:Plant Cell Rep;35(9):1907-16, 2016 Sep.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Overexpression of both native and mutant forms of AtCPK1 in Rubia cordifolia cells increased anthraquinone production and transcript abundance of the RcIPPI, RcOSBL, RcOSBS , and RcICS genes to different extents. Calcium-dependent protein kinases (CDPKs) are involved in various cell processes and are regulated by a calcium signal system. CDPKs also function in plant defense against stress factors such as pathogens, temperature, and salinity. In this study, we compared the effect of heterologous expression of two forms of the Arabidopsis AtCPK1 gene, native and constitutively active (Ca(2+)-independent), on anthraquinone production in transgenic Rubia cordifolia cells. Significant qualitative and quantitative differences were found in the content of anthraquinone derivatives in control and AtCPK1-transgenic calli. Expression of the AtCPK1 gene increased anthraquinone production by 3 and 12 times for native and constitutively active forms, respectively, compared with control cells. In addition, we identified and quantified the expression of genes encoding key enzymes of the anthraquinone biosynthesis pathway, including isochorismate synthase (ICS), o-succinylbenzoate synthase (OSBS), o-succinylbenzoate ligase (OSBL), and isopentenyl diphosphate isomerase (IPPi). In all AtCPK1-transgenic cell lines, expression of ICS, OSBS, OSBL, and IPPi increased considerably at 14-15 days of subculture and decreased at the end of cultivation (30 days). The results suggest that both native and constitutively active AtCPK1 forms induced anthraquinone accumulation at the logarithmic growth stage via enhancement of expression of genes involved in the metabolism of anthraquinones or their regulatory mechanisms.
[Mh] Termos MeSH primário: Antraquinonas/metabolismo
Proteínas de Arabidopsis/genética
Arabidopsis/genética
Genes de Plantas
Proteínas Quinases/genética
Rubia/genética
Rubia/metabolismo
Transformação Genética
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/metabolismo
Biomassa
Cromatografia Líquida de Alta Pressão
Regulação da Expressão Gênica de Plantas
Espectrometria de Massas
Fenótipo
Plantas Geneticamente Modificadas
Proteínas Quinases/metabolismo
Rubia/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Arabidopsis Proteins); EC 2.7.- (Protein Kinases); EC 2.7.1.- (CPK1 protein, Arabidopsis)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160603
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2005-z


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[PMID]:27208504
[Au] Autor:Veremeichik G; Bulgakov V; Shkryl Y
[Ad] Endereço:Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia.
[Ti] Título:Modulation of NADPH-oxidase gene expression in rolB-transformed calli of Arabidopsis thaliana and Rubia cordifolia.
[So] Source:Plant Physiol Biochem;105:282-289, 2016 Aug.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Expression of rol genes from Agrobacterium rhizogenes induces reprogramming of transformed plant cells and provokes pleiotropic effects on primary and secondary metabolism. We have previously established that the rolB and rolC genes impair reactive oxygen species (ROS) generation in transformed cells of Rubia cordifolia and Arabidopsis thaliana. In the present investigation, we tested whether this effect is associated with changes in the expression levels of NADPH oxidases, which are considered to be the primary source of ROS during plant-microbe interactions. We identified two full-length NADPH oxidase genes from R. cordifolia and examined their expression in non-transformed and rolB-transformed calli. In addition, we examined the expression of their homologous genes from A. thaliana in non-transformed and rolB-expressing cells. The expression of Rboh isoforms was 3- to 7-fold higher in both R. cordifolia and A. thaliana rolB-transformed cells compared with non-transformed cells. Our results for the first time show that Agrobacterium rolB gene regulates particular NADPH oxidase isoforms.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Proteínas de Bactérias/metabolismo
Regulação Enzimológica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
NADPH Oxidases/genética
Rubia/genética
Transformação Genética
beta-Glucosidase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/enzimologia
Arabidopsis/genética
NADPH Oxidases/química
NADPH Oxidases/metabolismo
Filogenia
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
Rubia/enzimologia
Alinhamento de Sequência
Estresse Fisiológico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Plant Proteins); EC 1.6.3.- (NADPH Oxidases); EC 3.2.1.- (RolB protein, Agrobacterium rhizogenes); EC 3.2.1.21 (beta-Glucosidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE


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[PMID]:25926096
[Au] Autor:Anantharaman A; Priya RR; Hemachandran H; Akella S; Rajasekaran C; Ganesh J; Fulzele DP; Siva R
[Ad] Endereço:School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, 632014, India.
[Ti] Título:Toxicity study of dibutyl phthalate of Rubia cordifolia fruits: in vivo and in silico analysis.
[So] Source:Environ Toxicol;31(9):1059-67, 2016 Sep.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Natural toxins from plant sources with wide ranges of biological activities reflect the upswing of drug design in the pharmaceutical industry. Rubia cordifolia L. is one of the most important red dye yielding plants. Most of the former researches have focused on the bioactive compounds from the roots of R. cordifolia, while no attention was paid towards the fruits. For the first time, here we report the presence of dibutyl phthalate in the fruits of R. cordifolia. Structural characterization was carried out using Ultraviolet-Visible spectrophotometer (UV-Vis), Fourier transform infrared (FTIR), gas chromatography-mass spectrophotometer (GC-MS), Nuclear magnetic resonance (NMR). Acute toxicity of the crude ethanolic extracts of the R. cordifolia fruits was examined in Swiss albino mice. No mortality was observed in all treated mice with 100, 500, 1000 mg/kg body weight of crude extract of R. cordifolia fruit and it indicates that the LD50 value is higher than 1000 mg/kg body weight. This study exhibited a significant change in the body weight. Alanine transaminase (ALT), total protein, triglycerides, glucose, and also the histopathological analysis of liver for all treated mice showed difference from the control group. The dibutyl phthalate was further evaluated for the toxicity study through in silico analysis. Together, the results highlighted that the toxic potential of R. cordifolia fruits extracts and also the toxicity profile of the fruit should be essential for the future studies dealing with the long term effect in animals. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1059-1067, 2016.
[Mh] Termos MeSH primário: Dibutilftalato/toxicidade
Fígado/efeitos dos fármacos
Extratos Vegetais/química
Rubia/química
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Animais
Peso Corporal/efeitos dos fármacos
Dibutilftalato/química
Dibutilftalato/isolamento & purificação
Feminino
Frutas/química
Frutas/metabolismo
Cromatografia Gasosa-Espectrometria de Massas
Fígado/metabolismo
Fígado/patologia
Espectroscopia de Ressonância Magnética
Camundongos
Raízes de Plantas/química
Raízes de Plantas/metabolismo
Rubia/metabolismo
Espectroscopia de Infravermelho com Transformada de Fourier
Testes de Toxicidade Aguda
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Triglycerides); 2286E5R2KE (Dibutyl Phthalate); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150501
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22115


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[PMID]:26697688
[Au] Autor:Yang HS; Wang J; Guo C; Liu W; Chen YY; Wei JF; Kang WY
[Ti] Título:Simultaneous determination of alizarin and rubimaillin in Rubia cordifolia by ultrasound-assisted ionic liquid-reversed phase liquid chromatography.
[So] Source:Zhongguo Zhong Yao Za Zhi;40(13):2617-23, 2015 Jul.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Four kinds of ionic liquids were adopted to analyze the content of rubimaillin and alizarin in Rubia cordifolia roots with ultrasonic-assisted extraction coupled with HPLC. The chromatographic column, Purospher star RP-C18 (4.6 mm x 250 mm, 5 microm), was used. Methanol and 0.4% acetic acid-water as mobile phase with flow rate at 0.85 mL min(-1), gradient elution, detection wavelength at 250 nm, chromatographic column temperature was controlled at room temperature. The result showed that rubimaillin and alizarin had the highest extraction yield when the [ HMIM] PF6methanol solution concentration of 0.6 mol x L(-1) as extraction solvent and the conditions were solid-liquid ratio of 1:80 (g x mL(-1)). Under the optimal extraction conditions, the content of alizarin from 0.01 to 0.04 microg showed a good linearity (r = 0.9999), the average recovery was 97.12%, the content of rubimaillin from 0.41 to 1.35 microg showed a good linearity (r = 0.9999), the average recovery was 98.10%. This experiment adopted environmentally friendly reagent as extraction solvent, the extraction efficiency was improved, and the environmental pollution caused by organic solvent was avoided, the harm of human body aslo was reduced. This method was simple and reliable, its repeatability was also very good, which had an important significance in the study of traditional Chinese medicine active ingredient extraction methods.
[Mh] Termos MeSH primário: Antraquinonas/análise
Cromatografia de Fase Reversa/métodos
Líquidos Iônicos/química
Piranos/análise
Rubia/química
[Mh] Termos MeSH secundário: Ultrassom
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Ionic Liquids); 0 (Pyrans); 55481-88-4 (rubimaillin); 60MEW57T9G (alizarin)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:151224
[Lr] Data última revisão:
151224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151225
[St] Status:MEDLINE



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