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[PMID]:28475604
[Au] Autor:Honório ICG; Bertoni BW; Telles MPC; Braga RDS; França SC; Coppede JDS; Correa VSC; Diniz Filho JAF; Pereira AMS
[Ad] Endereço:Universidade Estadual Paulista "Júlio de Mesquita Filho", Campus Lageado, Botucatu, São Paulo, Brazil.
[Ti] Título:Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon.
[So] Source:PLoS One;12(5):e0177103, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Uncaria tomentosa (Willd. ex Schult.) DC., a plant native to the Amazon region, is used widely in popular medicine and by the pharmaceutical industry because of its anti-inflammatory activity. However, the survival of this species is endangered by deforestation and indiscriminate collection, and a preservation plan is urgently required. The objectives of this study were to determine the genetic and chemical variability between and within eight populations of U. tomentosa from the Brazilian states of Acre, Pará and Amapá, and to investigate possible correlations between genetic and geographical distances, and between geographical distances or altitude and the accumulation of bioactive oxindole alkaloids. Three sequence-related amplified polymorphism (SRAP) markers were employed to fingerprint genomic DNA, and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high-performance liquid chromatography. Although significant divergence existed between the tested populations (FST = 0.246), the largest genetic diversity and the highest percentage of polymorphism (95.68%) was found within the population from Mâncio Lima, Acre. Gene flow was considered rather limited (Nm = 1.57), and no correlations between genetic and geographical distances were detected, suggesting that population structure followed an island model. Accumulations of mitraphylline and isomitraphylline varied in the range 32.94 to 0.57 and 3.75 to 0.36 mg g-1 dry weight, respectively. The concentration of isomitraphylline was positively influenced by altitude, such that the population collected at the site with the highest elevation (Tarauacá, Acre) exhibited the greatest alkaloid content. SRAP markers were very efficient in fingerprinting genomic DNA from U. tomentosa populations and clearly showed that genetic variability within populations was greater than between populations. A conservation and management plan should prioritize the creation of germplasm banks to prevent the loss of existing genetic variability, particularly within alkaloid-rich populations such as those of Tarauacá.
[Mh] Termos MeSH primário: Unha-de-Gato/química
Unha-de-Gato/genética
Variação Genética
Alcaloides de Indol/análise
[Mh] Termos MeSH secundário: Brasil
Casca de Planta/química
Extratos Vegetais/química
Floresta Úmida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indole Alkaloids); 0 (Plant Extracts); 0 (isomitraphylline); 1H9SRL2456 (mitraphylline)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177103


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[PMID]:28417940
[Au] Autor:Kosmider A; Czepielewska E; Kuras M; Gulewicz K; Pietrzak W; Nowak R; Nowicka G
[Ad] Endereço:Department of Biochemistry and Pharmacogenomics, Faculty of Pharmacy with the Laboratory Medicine Division, and Center for Preclinical Studies, Medical University of Warsaw, 02097 Warsaw, Poland. anita.kosmider@wum.edu.pl.
[Ti] Título:Uncaria tomentosa Leaves Decoction Modulates Differently ROS Production in Cancer and Normal Cells, and Effects Cisplatin Cytotoxicity.
[So] Source:Molecules;22(4), 2017 Apr 12.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is a woody vine with a long history of use in traditional Peruvian medicine and nowadays supplements containing this vine as ingredient are available. Immunomodulating, anti-inflammatory and anticancer properties of have been suggested and attributed mainly to the presence of tetracyclic or pentacyclic oxindole alkaloids. However, the synergic action of different compounds occurring in extracts and modulation of redox processes may significantly influence the anticancer activity of . The aim of the present study was to investigate for the first time the cytotoxic effects of the tetracyclic alkaloids free aqueous extract (decoction) of dried leaf blades in normal and cancer cells, and to assess the effect of the tested extract on cisplatin (CDDP) cytotoxicity. Tested extract was not cytotoxic for NHDF cells, but demonstrated cytotoxic effect against HepG2 cells. The extract increased ROS production in HepG2 cells, which resulted in decreased GSH level, leading to apoptosis of these cells through activation of caspase-3 and caspase-7. A reduction of NF-κB active form was observed in cancer cells. In normal cells the extract did not affect ROS production, GSH level and NF-κB activity, and maintained cell viability. HepG2 cells incubation with decoction and simultaneously with CDDP resulted in an increase in CDPP cytotoxic activity against HepG2, while under the same conditions prevents NHDF cell viability reduction due to CDDP. The results indicate that leaves decoction modulates differently cancer and normal cells oxidative metabolism and, enhanced cytotoxicity of CDDP against cancer cells and at the same time increased normal healthy cells resistance to cisplatin. Further studies are needed to confirm our observations and to describe underlying molecular mechanism, and the potential usefulness of decoction in adjuvant therapy for cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Unha-de-Gato/química
Oxirredução/efeitos dos fármacos
Extratos Vegetais/farmacologia
Folhas de Planta/química
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Apoptose/efeitos dos fármacos
Caspase 3/metabolismo
Caspase 7/metabolismo
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cisplatino/farmacologia
Células Hep G2
Seres Humanos
NF-kappa B/metabolismo
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Reactive Oxygen Species); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE


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[PMID]:28009448
[Au] Autor:Chang CW; Yeh YY; Chang LC; Hsu MC; Wu YT
[Ad] Endereço:School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
[Ti] Título:Rapid determination of oxindole alkaloids in cat's claw by HPLC using ionic liquid-based microwave-assisted extraction and silica monolithic column.
[So] Source:Biomed Chromatogr;31(8), 2017 Aug.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cat's claw is a large woody vine with hook-like thorns, and has been traditionally used to treat inflammatory disorders in South and Central America. In this study, a rapid, validated high-performance liquid chromatographic (HPLC) method using a silica monolithic column was developed for the simultaneous determination of oxindole alkaloids, namely rhynchophylline, pteropodine, isomitraphylline and isopteropodine, in cat's claw. The ionic liquid-based microwave-assisted extraction (ILMAE), considered as an environmentally friendly and powerful tool, was first applied in the extraction of oxindole alkaloids. To optimize the HPLC method, the stationary phases, pH values of mobile phase and flow rates were investigated. The validated HPLC method using a Monolithic RP column (100 × 4.6 mm) enables these analytes to be separated almost twice as fast as with a conventional particulate column (~16 vs ~30 min) with limits of quantification and detection of 0.5 and 0.15 µg/mL, respectively. The ILMAE conditions were optimized by the Taguchi orthogonal array design. In comparison with conventional water boiling extraction, ILMAE offers almost four times higher yields within an extremely short extraction time. The developed HPLC coupled with ILMAE method could be efficient and practical for rapid determination of oxindole alkaloids in cat's claw.
[Mh] Termos MeSH primário: Alcaloides/análise
Unha-de-Gato/química
Cromatografia Líquida de Alta Pressão/métodos
Alcaloides de Indol/análise
Indóis/análise
Compostos de Espiro/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/economia
Cromatografia Líquida de Alta Pressão/instrumentação
Líquidos Iônicos/química
Limite de Detecção
Micro-Ondas
Dióxido de Silício/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkaloids); 0 (Indole Alkaloids); 0 (Indoles); 0 (Ionic Liquids); 0 (Spiro Compounds); 0 (isomitraphylline); 0 (uncarine C); 0 (uncarine E); 46BQ79VJ8D (rhyncophylline); 7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3925


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[PMID]:27931150
[Au] Autor:Moraes RC; Carvalho AR; Lana AJ; Kaiser S; Pippi B; Fuentefria AM; Ortega GG
[Ad] Endereço:a Post-Graduate Program in Pharmaceutical Sciences (PPGCF), Faculty of Pharmacy , Universidade Federal do Rio Grande do Sul (UFRGS) , Porto Alegre , RS , Brazil.
[Ti] Título:In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non-Candida albicans isolates.
[So] Source:Pharm Biol;55(1):406-415, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Uncaria tomentosa D.C. (Rubiaceae) has several biological activities, including activity against resistant Candida strains. The synergistic interaction with terbinafine or fluconazole can be an important alternative to overcome this resistance. OBJECTIVES: The potential synergy between a water insoluble fraction (WIF) from Uncaria tomentosa bark and the antifungals terbinafine (TRB) and fluconazole (FLZ) against non-Candida albicans resistant strains was investigated. MATERIALS AND METHODS: TRB and FLZ, alone and combined with WIF, were tested by the checkerboard procedure using the micro-dilution technique against seven isolates of Candida glabrata and C. krusei. The molecular interactions occurring outside the cell wall were evaluated by scanning electron microscopy, Fourier transform infrared (FT-IR) and differential scanning calorimetry (DSC) analysis. RESULTS: The checkerboard inhibitory assay demonstrated synergy for WIF:TRB and WIF:FLZ combinations, respectively. The best synergistic cell damage was demonstrated unequivocally for the associations of WIF and TRB (1.95:4.0 µg/mL) and WIF and FLZ (1.95:8.0 µg/mL). The comparison of the FT-IR spectra of the antifungal alone, and in combination with WIF, allows recognizing clear differences in 3000, 1600, 1400, and 700-800 cm bands. Additionally, modifications on TRB and FLZ thermograms were clearly noticed after their combination with WIF. CONCLUSIONS: DSC and infrared analysis demonstrated intermolecular interactions between WIF and either TRB or FLZ. Hence, quite likely the synergistic effect is related to interaction events occurring outside the cell wall between antifungal and cat's claw proanthocyanidins. A direct action on the cell wall is suggested, without connection with the ABC efflux pump mechanism.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida/efeitos dos fármacos
Unha-de-Gato/química
Farmacorresistência Fúngica/efeitos dos fármacos
Fluconazol/farmacologia
Naftalenos/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/isolamento & purificação
Varredura Diferencial de Calorimetria
Candida/crescimento & desenvolvimento
Candida/ultraestrutura
Parede Celular/efeitos dos fármacos
Parede Celular/ultraestrutura
Sinergismo Farmacológico
Microscopia Eletrônica de Varredura
Fitoterapia
Casca de Planta
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Solubilidade
Espectroscopia de Infravermelho com Transformada de Fourier
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Naphthalenes); 0 (Plant Extracts); 059QF0KO0R (Water); 8VZV102JFY (Fluconazole); G7RIW8S0XP (terbinafine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE


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[PMID]:28074748
[Au] Autor:Schuh MJ
[Ti] Título:Possible Parkinson's Disease Induced by Chronic Manganese Supplement Ingestion.
[So] Source:Consult Pharm;31(12):698-703, 2016 Dec 01.
[Is] ISSN:0888-5109
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The objective is to report a case of possible neurotoxicity resulting from an incorrect dietary supplement for osteoporosis taken at a toxic dose. SUMMARY: The case study examined here is a 37-year-old African-American female who consumed excessive manganese over a period of years, resulting in Parkinson's disease (PD). This patient was referred to the pharmacist pharmacotherapy service by a neurology physician. PD has been shown in the medical literature to be caused by chronic exposure to high levels of manganese. It may be concluded that daily doses of manganese well above the upper limit of 9 mg per day were taken by this patient for an extended period of time, possibly causing PD via manganism. CONCLUSION: This case illustrates the unknown risks taken by patients who use excessive amounts of over-the-counter herbals and supplements and how pharmacists can assist patients and physicians in the proper use of these popular products. ABBREVIATIONS: AI = Adequate intake, EMS = Eosinophilia myalgia syndrome, MTM = Medication therapy management, UL = Tolerable upper limit.
[Mh] Termos MeSH primário: Suplementos Nutricionais/toxicidade
Manganês/toxicidade
Conduta do Tratamento Medicamentoso/organização & administração
Doença de Parkinson Secundária/induzido quimicamente
[Mh] Termos MeSH secundário: Adulto
Unha-de-Gato
Relação Dose-Resposta a Droga
Eucalyptus
Feminino
Seres Humanos
Preparações de Plantas/administração & dosagem
Preparações de Plantas/efeitos adversos
Serotonina/administração & dosagem
Serotonina/efeitos adversos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Preparations); 333DO1RDJY (Serotonin); 42Z2K6ZL8P (Manganese)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.4140/TCP.n.2016.698


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[PMID]:27590731
[Au] Autor:Santos KF; Gutierres JM; Pillat MM; Rissi VB; Santos Araújo MD; Bertol G; Gonçalves PB; Schetinger MR; Morsch VM
[Ad] Endereço:Department of Biochemistry and Molecular Biology, CCNE, Federal University of Santa Maria (UFSM), Avenida Roraima, Prédio 18, CEP 97105-900 Santa Maria, RS, Brazil; Department of Health Sciences, Regional Integrada University (URI), CEP 984000-000 Frederico Westphalen, RS, Brazil. Electronic address
[Ti] Título:Uncaria tomentosa extract alters the catabolism of adenine nucleotides and expression of ecto-5'-nucleotidase/CD73 and P2X7 and A1 receptors in the MDA-MB-231 cell line.
[So] Source:J Ethnopharmacol;194:108-116, 2016 Dec 24.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHOPHARMACOLOGICAL RELEVANCE: Uncaria tomentosa (Willd.) DC. (Rubiaceae) (Ut), also known as cat's claw, is a woody liana widely spread throughout the Amazon rainforest of Central and South America, containing many chemical constituents such as oxindole alkaloids, which are responsible for various biological activities. Since ancient times, the indigenous people of Peru have used it as a bark infusion for the treatment of a wide range of health problems gastric ulcers, arthritis and rheumatism. Recently, Ut is distributed worldwide and used as an immunomodulatory and anti-inflammatory herbal remedy. Additionally, U. tomentosa also has antitumural activity. However, little is known about the action of U. tomentosa on the purinergic system mechanisms, which is involved in tumor progression. AIM OF THE STUDY: Considering the pharmacological properties of U. tomentosa, we sought to evaluate the hydroalcoholic extract U tomentosa is able to influence the purinergic system in breast cancer cells, MDA-MB-231. Through the activity and expression of ectonucleotidases (NTPDase - CD39; Ecto-5'-nucleotidase - CD73) and purinergic repceptores (P2X7 and A1). MATERIALS AND METHODS: A hydroalcoholic extract was prepared in two concentrations, 250 and 500µg/mL. (Ut250; Ut500). The effect of these concentrations on the activity and expression of ectonucleotidases, as well as on the density of purinergic receptors were investigated in MDA-MB-231 breast cancer cells. Cells were treated with the hydroalcoholic extract of Uncaria tomentosa and/or doxorubicin (Doxo 1µM; Ut250+Doxo; Ut500+Doxo) for 24h. RESULTS: Although the results were not significant for the hydrolysis of the ATP, they presented an increase in the ADP hydrolysis in the Ut500+Doxo group when compared to the control group. Additionally, the activity of 5'-nucleotidase was inhibited in all groups when compared with the untreated group of cells. Inhibition of the enzyme was more evident in groups with U. tomentosa per se. The expression of CD39 was increased in the Ut250 and Ut250+Doxo groups when compared to the control group. No changes were found in the CD73 expression. Furthermore, a reduction in the density of the P2X7 receptor in all treated groups was detected. On the other hand, the density of the A1 receptor increased in all groups compared to the control group, with the exception of the Ut500+Doxo group. CONCLUSION: Therefore, we conclude that hydroalcoholic extract of U. tomentosa may be responsible for the reduction of adenosine levels in the extracellular medium, which accelerates tumor progression. Interestingly, the dysregulation of A1 and P2X7 receptors in the MDA-MB-231 cells exacerbate the proliferation of this cells and U. tomentosa treatment may be stimulate the antitumor activity of adenosine A1 receptor and control the P2X7 effects. Our study demonstrates the significant participation of purinergic pathway in the regulation of MDA-MB-231 progression; additionally, U. tomentosa treatment alone or combined with chemotherapy may favor the action of doxorubicin.
[Mh] Termos MeSH primário: 5´-Nucleotidase/metabolismo
Nucleotídeos de Adenina/metabolismo
Unha-de-Gato/química
Receptor A1 de Adenosina/metabolismo
Receptores Purinérgicos P2X7/metabolismo
[Mh] Termos MeSH secundário: Neoplasias da Mama/enzimologia
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Linhagem Celular Tumoral
Seres Humanos
Extratos Vegetais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adenine Nucleotides); 0 (Plant Extracts); 0 (Receptor, Adenosine A1); 0 (Receptors, Purinergic P2X7); EC 3.1.3.5 (5'-Nucleotidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE


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[PMID]:27209428
[Au] Autor:Moura Mdel G; Lopes LC; Biavatti MW; Busse JW; Wang L; Kennedy SA; Bhatnaga N; Bergamaschi Cde C
[Ad] Endereço:Department of Pharmaceutical Sciences, University of Sorocaba, Sorocaba, São Paulo, Brazil.
[Ti] Título:Brazilian oral herbal medication for osteoarthritis: a systematic review protocol.
[So] Source:Syst Rev;5:86, 2016 May 21.
[Is] ISSN:2046-4053
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Osteoarthritis affects 1 % of the world's population and is the most common cause of musculoskeletal impairment in the elderly. Herbal medications are commonly used in Brazil to manage symptoms associated with osteoarthritis, and some of them are financed by the Brazilian government; however, the effectiveness of most of these agents is uncertain. The aim was to systematically review the efficacy and safety of 13 oral herbal medications used in Brazil for the treatment of osteoarthritis. METHODS: Randomized clinical trials eligible for our systematic review will enroll adults with osteoarthritis treated by a Brazilian herbal medication or a control group (placebo or active control). Using terms to include all forms of osteoarthritis combined with herbal medications, we will search the following electronic databases: Cochrane Central Register of Controlled Trials (CENTRAL); MEDLINE; EMBASE; CINAHL; Web of Science; Health Star; AMED, the database of the Cochrane Complementary Medicine Field, LILACS; CAB abstracts, Clinical trial.gov, WHO trials registry, and Bank of Brazil Thesis (CAPES), to 31 January 2016, without restrictions concerning language or status of publication. Outcomes of interest include the following: symptom relief (e.g., pain), adverse events (gastrointestinal bleeding, epigastric pain, nausea, and allergic reactions), discontinuation due to adverse events, quality of life, and the satisfaction with the treatment. Dichotomous data will be summarized as risk ratios; continuous data will be given as standard average differences with 95 % confidence intervals. A team of reviewers will assess each citation independently for eligibility and in duplicate it. For eligible studies, the same reviewers will perform data extraction, bias risk assessment, and determination of the overall quality of evidence for each of the outcomes using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) classification system. DISCUSSION: This is the first study that will evaluate the use of herbal medications used in Brazil for the treatment of pain caused by osteoarthritis. The results could guide prescribers in decision-making in clinical practice, to inform the patients with pain caused by osteoarthritis in relation to effective and safe treatment options and to inform the managers of the public health system which of the plants could actually be financed by the Brazilian government. SYSTEMATIC REVIEW REGISTRATION: PROSPERO 42015019793.
[Mh] Termos MeSH primário: Osteoartrite/tratamento farmacológico
Fitoterapia
Preparações de Plantas/uso terapêutico
[Mh] Termos MeSH secundário: Boswellia
Brasil
Unha-de-Gato
Chenopodium ambrosioides
Cordia
Curcuma
Fabaceae
Harpagophytum
Seres Humanos
Persea
Salix
Resultado do Tratamento
Uncaria
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Plant Preparations)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160523
[St] Status:MEDLINE
[do] DOI:10.1186/s13643-016-0261-1


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[PMID]:27180878
[Au] Autor:Kaiser S; Carvalho ÂR; Pittol V; Dietrich F; Manica F; Machado MM; de Oliveira LF; Oliveira Battastini AM; Ortega GG
[Ad] Endereço:Laboratório de Desenvolvimento Galênico (LDG), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. Electronic address: samokaiser@yahoo.com.br.
[Ti] Título:Genotoxicity and cytotoxicity of oxindole alkaloids from Uncaria tomentosa (cat's claw): Chemotype relevance.
[So] Source:J Ethnopharmacol;189:90-8, 2016 Aug 02.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Uncaria tomentosa (Willdenow ex Roemer & Schultes) DC. (Rubiaceae) or cat's claw is a climber vine from the South American rainforest used in folk medicine for cancer treatment. Its antitumor activity has been mostly ascribed to pentacyclic oxindole alkaloids (POA) from stem bark and leaves while the activity of tetracyclic oxindole alkaloids (TOA) remains unknown. In recent times, the occurrence of three chemotypes based on its oxindole alkaloid profile was noticed in U. tomentosa, namely, chemotype I (POA cis D/E ring junction); chemotype II (POA trans D/E ring junction) or chemotype III (TOA). Consequently, the relationship between the chemotype and cytotoxic and genotoxic activities deserves attention. AIM OF THE STUDY: To evaluate the influence of cat's claw chemotypes on genotoxicity and cytotoxicity against non malignant and malignant human cell line models. MATERIAL AND METHODS: Four authentic stem bark cat's claw samples (SI-SIV) and two leaf samples (LII and LIII) were analyzed by HPLC-PDA, properly extracted and fractioned by ion-exchange to obtain oxindole alkaloid purified fractions (OAPFs). The freeze-dried fractions were assayed for genotoxicity and cytotoxicity against human leukocytes (non malignant cell line) by the micronuclei frequency method and the alkaline comet DNA assay, and the trypan blue method, respectively. Moreover, the cytotoxicity of each OAPF was evaluated against a human bladder cancer cell line (T24) and human glioblastoma cell line (U-251-MG) by MTT method (malignant cell lines). Additionally, the isomerization of oxindole alkaloids throughout the course of cell incubation was monitored by HPLC-PDA. RESULTS: Based on HPLC-PDA analyses, sample SI was characterized as chemotype I, while samples SII and LII were characterized as chemotype II, and samples SIII, SIV and LIII as chemotype III. The chemotypes showed comparable cytotoxic activity toward malignant cell lines (T24 and U-251-MG) unlike human leukocytes (non malignant cell line), where this activity was clearly distinct. Chemotype II (POA trans D/E ring junction) showed a higher selectivity index (SI) against malignant cells (SI=1.11-3.04) than chemotype I (SI=0.10-0.19) and III (SI=0.21-0.57). No important genotoxic potential was found by micronuclei frequency and alkaline comet DNA assays. Despite the isomerization of oxindole alkaloids during the cell incubation, the chemotype of the cat's claw samples remained unchanged. CONCLUSION: Cat's claw chemotypes showed different selectivity against human malignant cells, so that the correct identification of each chemotype seems to be important to better understand its antitumor potential.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Unha-de-Gato/química
Dano ao DNA
Alcaloides de Indol/farmacologia
Indóis/farmacologia
Leucócitos/efeitos dos fármacos
Neoplasias/tratamento farmacológico
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/toxicidade
Sobrevivência Celular/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Cromatografia por Troca Iônica
Ensaio Cometa
Relação Dose-Resposta a Droga
Seres Humanos
Alcaloides de Indol/química
Alcaloides de Indol/isolamento & purificação
Alcaloides de Indol/toxicidade
Indóis/química
Indóis/isolamento & purificação
Indóis/toxicidade
Concentração Inibidora 50
Leucócitos/patologia
Masculino
Micronúcleos com Defeito Cromossômico/induzido quimicamente
Testes para Micronúcleos
Estrutura Molecular
Neoplasias/patologia
Fitoterapia
Casca de Planta/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/toxicidade
Folhas de Planta/química
Plantas Medicinais
Medição de Risco
Relação Estrutura-Atividade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Indole Alkaloids); 0 (Indoles); 0 (Plant Extracts); 0S9338U62H (2-oxindole)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160517
[St] Status:MEDLINE


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Texto completo SciELO Brasil
[PMID]:27119588
[Au] Autor:Herrera DR; Durand-Ramirez JE; Falcão A; Silva EJ; Santos EB; Gomes BP
[Ad] Endereço:Endodontic Division, Department of Restorative Dentistry, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
[Ti] Título:Antimicrobial activity and substantivity of Uncaria tomentosa in infected root canal dentin.
[So] Source:Braz Oral Res;30(1):e61, 2016.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to analyze the antimicrobial activity and substantivity of Uncaria tomentosa Willd DC (cat's claw, CC) in root dentin contaminated with Enterococcus faecalis. Forty-eight human premolars were contaminated with E. faecalis (ATCC 29212) and randomly divided into four groups according to the irrigant used during chemomechanical preparation (CMP): CC group: 2% CC gel; CHX group: 2% chlorhexidine digluconate gel (CHX); NaOCl group: 5.25% sodium hypochlorite (NaOCl); and SS group: sterile saline (SS). Microbiological samples were collected before (S1) and after (S2) CMP and after 7 days (S3). Colony-forming units (CFU/mL) at the different sampling times and comparisons among the groups were statistically analyzed by Wilcoxon and Kruskal-Wallis tests (p < 0.05). Significant bacterial reduction was achieved in all groups after CMP (p < 0.05). Results show no significant difference between S3 and S2 (p > 0.05) in the CC and CHX groups. Bacterial load was higher in S3 than in S2 samples (p < 0.05) in the NaOCl and SS groups. Our results suggest antibacterial effect of 2% CC gel against E. faecalis in infected dentin, in addition to antibacterial substantivity of 2% CC and 2% CHX up to 7 days.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Unha-de-Gato/química
Cavidade Pulpar/efeitos dos fármacos
Cavidade Pulpar/microbiologia
Dentina/efeitos dos fármacos
Dentina/microbiologia
Enterococcus faecalis/efeitos dos fármacos
[Mh] Termos MeSH secundário: Carga Bacteriana/efeitos dos fármacos
Clorexidina/análogos & derivados
Clorexidina/farmacologia
Contagem de Colônia Microbiana
Seres Humanos
Distribuição Aleatória
Valores de Referência
Irrigantes do Canal Radicular/farmacologia
Hipoclorito de Sódio/farmacologia
Estatísticas não Paramétricas
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Root Canal Irrigants); DY38VHM5OD (Sodium Hypochlorite); MOR84MUD8E (chlorhexidine gluconate); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE


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[PMID]:26926175
[Au] Autor:Montserrat-de la Paz S; Fernandez-Arche A; de la Puerta R; Quilez AM; Muriana FJ; Garcia-Gimenez MD; Bermudez B
[Ad] Endereço:Department of Pharmacology, School of Pharmacy, University of Seville, 41012, Seville, Spain ; Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, CSIC, Seville, Spain.
[Ti] Título:Mitraphylline inhibits lipopolysaccharide-mediated activation of primary human neutrophils.
[So] Source:Phytomedicine;23(2):141-8, 2016 Feb 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mitraphylline (MTP) is the major pentacyclic oxindolic alkaloid presented in Uncaria tomentosa. It has traditionally been used to treat disorders including arthritis, heart disease, cancer, and other inflammatory diseases. However, the specific role of MTP is still not clear, with more comprehensivestudies, our understanding of this ancient herbal medicine will continue growing. HYPOTHESIS/PURPOSE: Some studies provided its ability to inhibit proinflamatory cytokines, such as TNF-α, through NF-κB-dependent mechanism. TNF-α primes neutrophils and modulates phagocytic and oxidative burst activities in inflammatory processes. Since, neutrophils represent the most abundant pool of leukocytes in human blood and play a crucial role in inflammation, we aimed to determine the ability of MTP to modulate neutrophil activation and differentially regulate inflammatory-related cytokines. METHODS: To determine the mechanism of action of MTP, we investigated the effects on LPS-activated human primary neutrophils responses including activation surface markers by FACS and the expression of inflammatory cytokines, measured by real time PCR and ELISA. RESULTS: Treatment with MTP reduced the LPS-dependent activation effects. Activated neutrophils (CD16(+)CD62L(-)) diminished after MTP administration. Moreover, proinflamatory cytokines (TNF-α, IL-6 or IL-8) expression and secretion were concomitantly reduced, similar to basal control conditions. CONCLUSION: Taken together, our results demonstrate that MTP is able to elicit an anti-inflammatory response that modulates neutrophil activation contributing to the attenuation of inflammatory episodes. Further studies are need to characterize the mechanism by which MTP can affect this pathway that could provide a means to develop MTP as new candidate for inflammatory disease therapies.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Citocinas/metabolismo
Alcaloides de Indol/farmacologia
Neutrófilos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Unha-de-Gato/química
Seres Humanos
Interleucina-6/metabolismo
Interleucina-8/metabolismo
Lipopolissacarídeos
NF-kappa B/metabolismo
Neutrófilos/citologia
Casca de Planta/química
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (IL6 protein, human); 0 (IL8 protein, human); 0 (Indole Alkaloids); 0 (Interleukin-6); 0 (Interleukin-8); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha); 1H9SRL2456 (mitraphylline)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE



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