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Pesquisa : B01.650.940.800.575.912.250.462.750 [Categoria DeCS]
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[PMID]:29351747
[Au] Autor:Park SM; Min BG; Jung JY; Jegal KH; Lee CW; Kim KY; Kim YW; Choi YW; Cho IJ; Ku SK; Kim SC
[Ad] Endereço:College of Korean Medicine, Daegu Haany University, Gyeongsan, 38610, Republic of Korea.
[Ti] Título:Combination of Pelargonium sidoides and Coptis chinensis root inhibits nuclear factor kappa B-mediated inflammatory response in vitro and in vivo.
[So] Source:BMC Complement Altern Med;18(1):20, 2018 Jan 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pelargonium sidoides (PS) and Coptis chinensis root (CR) have traditionally been used to treat various diseases, including respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders. The present study was conducted to evaluate the anti-inflammatory effects of a combination of PS and CR in vitro and in vivo. METHODS: The in vitro effects of PS + CR on the induction of inflammation-related proteins were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The levels of nitric oxide (NO) and of inflammatory cytokines and prostaglandin E (PGE ) were measured using the Griess reagent and enzyme-linked immunosorbent assay (ELISA) methods, respectively. The expression of inflammation-related proteins was confirmed by Western blot. Additionally, the effects of PS + CR on paw edema volume, skin thickness, and numbers of infiltrated inflammatory cells, mast cells, COX-2-, iNOS-, and TNF-α-immunoreactive cells in dorsum and ventrum pedis skin were evaluated in a rat model of carrageenan (CA)-induced paw edema. RESULTS: PS + CR significantly reduced production of NO, PGE and three pro-inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß, and IL-6) and also decreased levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Treatment with PS + CR significantly reduced the protein expression levels of LPS-stimulated nuclear factor kappa B (NF-κB) and phosphorylated inhibitor of NF-κB (p-I-κBα). Additionally, PS + CR significantly inhibited the increases in paw swelling, skin thickness, infiltrated inflammatory cells, mast cell degranulation, COX-2-, iNOS-, and TNF-α-immunoreactive cells in the rat model of CA-induced acute edematous paw. CONCLUSIONS: These results demonstrate that PS + CR exhibits anti-inflammatory properties through decreasing the production of pro-inflammatory mediators (NO, PGE , TNF-α, IL-1ß, and IL-6), suppressing NF-κB signaling in LPS-induced RAW 264.7 cells. Additionally, the results of the CA-induced rat paw edema assay revealed an anti-edema effect of PS + CR. Furthermore, it is suggested that PS + CR also inhibits acute edematous inflammation by suppressing mast cell degranulation and inflammatory mediators (COX-2, iNOS, and TNF-α). Thus, PS + CR may be a potential candidate for the treatment of various inflammatory diseases, and it may also contribute to a better understanding of the molecular mechanisms underlying inflammatory response regulation.
[Mh] Termos MeSH primário: Coptis/química
Inflamação/metabolismo
NF-kappa B/metabolismo
Pelargonium/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Raízes de Plantas/química
[Mh] Termos MeSH secundário: Animais
Citocinas/metabolismo
Edema/metabolismo
Expressão Gênica/efeitos dos fármacos
Masculino
Mastócitos/efeitos dos fármacos
Camundongos
Óxido Nítrico/metabolismo
Células RAW 264.7
Ratos
Ratos Sprague-Dawley
Pele/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (NF-kappa B); 0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2088-x


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[PMID]:28407721
[Au] Autor:Seker Karatoprak G; Göger F; Yerer MB; Kosar M
[Ad] Endereço:a Department of Pharmacognosy, Pharmacology, Faculty of Pharmacy , Erciyes University , Kayseri , Turkey.
[Ti] Título:Chemical composition and biological investigation of Pelargonium endlicherianum root extracts.
[So] Source:Pharm Biol;55(1):1608-1618, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Pelargonium endlicherianum Fenzl. (Geraniaceae) roots and flowers are traditionally used in Turkey as a decoction treatment against intestinal parasites. Neither the chemical composition nor the potential bioactivity of the plant roots has been studied before. OBJECTIVES: The phenolic content and effects of P. endlicherianum root extracts on antioxidant enzyme levels on A549 cells were studied for the first time. MATERIALS AND METHODS: The chemical composition was analyzed via spectrophotometric and chromatographic (HPLC MS/MS and HPLC) techniques. The antioxidant activity was determined at different concentrations ranging from 0.001 to 2 mg/mL using DPPH and ABTS radical scavenging activity, ß-carotene-linoleic acid co-oxidation assay, protection of 2-deoxyribose and bovine brain-derived phospholipids against a hydroxyl radical-mediated degradation assay. Glutathione peroxidase and superoxide dismutase activities were also studied as well as the effects of the extracts on nitric oxide levels on IL-1ß stimulated A549 cells. RESULTS: The key parameters for the most active ethyl acetate extract included the following: DPPH IC : 0.23 mg/mL, TEAC/ABTS: 2.17 mmol/L Trolox, reduction: 0.41 mmol/g AsscE, and protection of lipid peroxidation IC : 0.05 mg/mL. Furthermore, the ethyl acetate extract increased the SOD level significantly compared to control group (4.48 U/mL) at concentrations of 100 and 200 µg/mL SOD, 5.50 and 5.67 U/mL, respectively. Apocynin was identified as the major component, and the ethyl acetate fraction was found to be rich in phenolic compounds. DISCUSSION AND CONCLUSION: Pelargonium endlicherianum root extracts displayed antioxidant activity and increased the antioxidant enzyme levels in IL-1ß stimulated A549 cells, while decreasing the NO levels.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Pelargonium/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Células A549
Animais
Antioxidantes/administração & dosagem
Antioxidantes/isolamento & purificação
Encéfalo/efeitos dos fármacos
Encéfalo/metabolismo
Bovinos
Cromatografia Líquida de Alta Pressão
Relação Dose-Resposta a Droga
Glutationa Peroxidase/metabolismo
Seres Humanos
Concentração Inibidora 50
Óxido Nítrico/metabolismo
Fosfolipídeos/metabolismo
Extratos Vegetais/administração & dosagem
Extratos Vegetais/química
Raízes de Plantas
Superóxido Dismutase/metabolismo
Espectrometria de Massas em Tandem
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phospholipids); 0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1314511


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[PMID]:28389086
[Au] Autor:Naveenkumar R; Muthukumar A; Sangeetha G; Mohanapriya R
[Ad] Endereço:Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India; Annamalai University, Annamalainagar, Chidambaram 608002, Tamil Nadu, India. Electronic address: pathonaveen92@yahoo.com.
[Ti] Título:Developing eco-friendly biofungicide for the management of major seed borne diseases of rice and assessing their physical stability and storage life.
[So] Source:C R Biol;340(4):214-225, 2017 Apr.
[Is] ISSN:1768-3238
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Three plant oils (Cymbopogon citratus, Cymbopogon martini, and Pelargonium graveolens) were developed as EC formulations and tested for their physical stabilities. EC formulations (10EC, 20EC and 30EC) of C. citratus, C. martini and P. graveolens had emulsion stability, spontaneity property, heat and cold stability. EC formulated plant oils were screened against the major seed borne fungi of rice such as Curvularia lunata, Fusarium moniliforme, Bipolaris oryzae, and Sarocladium oryzae. The level of inhibition varied among the concentrations of EC formulations. Among the three EC formulations, that of C. citratus oil 30EC recorded 100% inhibition on the mycelial growth of test pathogens. In the blotter paper method, rice seeds treated with a formulation of C. citratus oil 30EC controlled the infection of C. lunata, F. moniliforme, B. oryzae and S. oryzae in rice seed to the tune of 66.0%, 60.4%, 66.0% and 69.1%, respectively. Seed soaking with formulation of C. citratus oil 30EC showed the highest percentage of normal seedlings, the lowest number of abnormal seedling and fresh ungerminated seeds when tested with the roll-towel method. Seed soaking with 30EC formulation of C. citratus oil increased seed germination, shoot length, root length and vigour of rice seedlings when tested with the plastic tray method. Transmission of pathogens from seed to seedling was reduced significantly by the 30EC formulation of C. citratus oil when tested with the plastic pot method. The effect of the storage life of the 30EC formulation of C. citratus oil showed that it had retained their antifungal effect till the end of the incubation period (120 days), and is able to inhibit the mycelial growth of all test pathogens to the 100% level.
[Mh] Termos MeSH primário: Antifúngicos/química
Oryza
Doenças das Plantas/microbiologia
Óleos Vegetais/química
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Ascomicetos/efeitos dos fármacos
Cymbopogon/química
Estabilidade de Medicamentos
Fusarium/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Pelargonium/química
Óleos Vegetais/farmacologia
Sementes/efeitos dos fármacos
Sementes/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Oils)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE


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[PMID]:28110082
[Au] Autor:Benelli G; Pavela R; Canale A; Cianfaglione K; Ciaschetti G; Conti F; Nicoletti M; Senthil-Nathan S; Mehlhorn H; Maggi F
[Ad] Endereço:Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy. Electronic address: benelli.giovanni@gmail.com.
[Ti] Título:Acute larvicidal toxicity of five essential oils (Pinus nigra, Hyssopus officinalis, Satureja montana, Aloysia citrodora and Pelargonium graveolens) against the filariasis vector Culex quinquefasciatus: Synergistic and antagonistic effects.
[So] Source:Parasitol Int;66(2):166-171, 2017 Apr.
[Is] ISSN:1873-0329
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mosquito vector control is facing a number of important and timely challenges, mainly due to the rapid development of pesticide resistance and environmental concerns. In this scenario, screening of botanical resources for their mosquitocidal activity may offer effective and eco-friendly tools against Culicidae vectors. Culex quinquefasciatus Say (Diptera: Culicidae) is a vector of lymphatic filariasis and of dangerous arboviral diseases, such as West Nile and St. Louis encephalitis. In this study, the chemical composition of five essential oils obtained from different plants, namely Pinus nigra J.F. Arnold var. italica (Pinaceae), Hyssopus officinalis L. subsp. aristatus (Lamiaceae), Satureja montana L. subsp. montana (Lamiaceae), Aloysia citriodora Palau (Verbenaceae) and Pelargonium graveolens L'Hér (Geraniaceae), was investigated by GC-MS analysis. Furthermore, it was evaluated their acute toxicity on larvae of C. quinquefasciatus. Then, the most effective oils were selected, in order to focus on the potential synergistic and antagonistic effects, testing them in binary mixtures on C. quinquefasciatus larvae. Results showed that the higher effectiveness was obtained by S. montana subsp. montana essential oil (LC =25.6µL·L ), followed by P. nigra var. italica (LC =49.8µL·L ) and A. citriodora (LC =65.6µL·L ), while the other essential oils showed LC values higher than 90µL·L . The larvicidal effectiveness can be enhanced by preparing simple binary mixtures of essential oils, such as S. montana+A. citriodora (ratio 1:1), which showed higher larvicidal toxicity (LC =18.3µL·L ). On the other hand, testing S. montana+P. nigra (1:1) an antagonistic effect was detected, leading to a LC (72.5µL·L ) higher than the LC values calculated for the two oils tested separately. Overall, our results add useful knowledge to allow the employ of synergistic essential oil blends as effective, cheap and eco-friendly mosquito larvicides.
[Mh] Termos MeSH primário: Culex/efeitos dos fármacos
Inseticidas/farmacologia
Mosquitos Vetores/efeitos dos fármacos
Óleos Voláteis/farmacologia
Óleos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antagonismo de Drogas
Sinergismo Farmacológico
Filariose Linfática/prevenção & controle
Filariose Linfática/transmissão
Encefalite de St. Louis/prevenção & controle
Encefalite de St. Louis/transmissão
Cromatografia Gasosa-Espectrometria de Massas
Hyssopus/química
Inseticidas/química
Larva/efeitos dos fármacos
Óleos Voláteis/química
Pelargonium/química
Pinus/química
Folhas de Planta/química
Óleos Vegetais/química
Satureja/química
Verbenaceae/química
Febre do Nilo Ocidental/prevenção & controle
Febre do Nilo Ocidental/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Oils, Volatile); 0 (Plant Oils)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


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[PMID]:27768575
[Au] Autor:Lavasanijou MR; Sohrabi HR; Karimi M; Ashjazade MA; Salajeghe M; Farzineejadizadeh H; Parsaei P; Elmamooz A
[Ad] Endereço:Medical Education Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
[Ti] Título:Wound Healing Effects of Quercus Brantii and Pelargonium Graveolens Extracts in Male Wistar Rats.
[So] Source:Wounds;28(10):369-375, 2016 Oct.
[Is] ISSN:1943-2704
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Pelargonium/química
Extratos Vegetais/farmacologia
Quercus/química
Cicatrização/efeitos dos fármacos
Ferimentos e Lesões/tratamento farmacológico
Ferimentos e Lesões/patologia
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/farmacologia
Modelos Animais de Doenças
Masculino
Óleos Voláteis/química
Óleos Voláteis/farmacologia
Extratos Vegetais/química
Folhas de Planta/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Oils, Volatile); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE


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[PMID]:27717933
[Au] Autor:Fal AM; Conrad F; Schönknecht K; Sievers H; Pawinska A
[Ad] Endereço:Zaklad Ekonomiki i Organizacji Ochrony Zdrowia, Narodowy Instytut Zdrowia Publicznego - Panstwowy Zaklad Higieny, Warszawa, Poland, Katedra Zdrowia Publicznego Uniwersytet Medyczny, Wroclaw, Poland.
[Ti] Título:Antiviral activity of the "Virus Blocking Factor" (VBF) derived i.a. from Pelargonium extract and Sambucus juice against different human-pathogenic cold viruses in vitro.
[So] Source:Wiad Lek;69(3 pt 2):499-511, 2016.
[Is] ISSN:0043-5147
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:THE AIM: The in-vitro antiviral activity of the "Virus Blocking Factor" (VBF), a combination of Pelargonium extract and Sambucus juice with addition of Betaglucan 1,3 / 1,6, Zincum gluconium, Acidum ascorbicum, was studied against human pathogenic viruses: Influenza A H1N1 (FluA H1N1), Rhinovirus B subtype 14 (HRV14), Respiratory Syncytial Virus (RSV), Parainfluenzavirus subtype 3 (Para 3), and Adenovirus C subtype 5 (Adeno 5). METHOD: Antiviral activity was assessed using plaque-reduction assays after adding the test substance post infection of the MDCK, HeLa and HEp-2 cells with the viruses. Ribavirin Virazol and - in case of Adenovirus an internal laboratory standard - were used as positive controls. Cytotoxic effects of VBF and VBF Control onto the virus permissive MDCK, HeLa and HEp-2 cells were examined. Non-toxic concentrations of VBF were determined by the Methylthiazoletetrazolium test (MTT-Test). RESULTS AND CONCLUSIONS: In all antiviral studies VBF showed (2.1%) a dose-dependent antiviral activity against FluA H1N1 and HRV14 at non-toxic concentrations. A very strong effect was demonstrated in concentrations of 2.5% and 1.25% where replication of H1N1 and HRV14 was nearly completely blocked. Dose-dependent antiviral activity was detectable against RSV in a concentration range of 1.25% to 0.63% of the test item. Due to toxic side effects of a 2.5% concentration at least a "minor effect" of about 30% (1.25% solution) against Para 3 infected HEp-2 cells could be determined. Concerning Adeno 5 not any antiviral activity could be demonstrated in all studies with all tested substance concentrations of VBF. VBF Control did not show any cytotoxicity and antiviral effects. Further research is needed to elucidate clinical effect of VBF.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Pelargonium/química
Sambucus/química
Viroses/tratamento farmacológico
[Mh] Termos MeSH secundário: Adenovírus Humanos/efeitos dos fármacos
Animais
Linhagem Celular
Cães
Sucos de Frutas e Vegetais/análise
Seres Humanos
Extratos Vegetais/química
Raízes de Plantas/química
Vírus de RNA/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE


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[PMID]:27688091
[Au] Autor:Vidovic M; Morina F; Prokic L; Milic-Komic S; Zivanovic B; Jovanovic SV
[Ad] Endereço:Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Kneza Viseslava 1, 11030 Belgrade, Serbia. Electronic address: marija@imsi.rs.
[Ti] Título:Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H O in (peri)vascular tissue induced by sunlight and paraquat.
[So] Source:J Plant Physiol;206:25-39, 2016 Nov 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study we exposed variegated leaves of Pelargonium zonale to strong sunlight (>1100µmolm s of photosynthetically active radiation) with and without paraquat (Pq), with the aim to elucidate the mechanisms of H O regulation in green and white tissues with respect to the photosynthetically-dependent generation of reactive oxygen species (ROS). Sunlight induced marked accumulation of H O in the apoplast of vascular and (peri)vascular tissues only in green sectors. This effect was enhanced by the addition of Pq. In the presence of diphenyl iodide, an NADPH oxidase inhibitor, H O accumulation was abolished. Distinct light-induced responses were observed: in photosynthetic cells, sunlight rapidly provoked ascorbate (Asc) biosynthesis and an increase of glutathione reductase (GR) and catalase activities, while in non-photosynthetic cells, early up-regulation of soluble ascorbate peroxidase, dehydroascorbate reductase (DHAR) and GR activities was observed. Paraquat addition stimulated DHAR and GR activities in green sectors, while in white sectors activities of monodehydroascorbate reductase, DHAR and class III peroxidases, as well as Asc content rapidly increased. Differential antioxidative responses in the two tissues in the frame of their contrasting metabolisms, and the possible role of (peri)vascular H O in signaling were discussed.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Peróxido de Hidrogênio/metabolismo
Paraquat/toxicidade
Pelargonium/efeitos dos fármacos
Pelargonium/metabolismo
Folhas de Planta/metabolismo
Feixe Vascular de Plantas/metabolismo
Luz Solar
[Mh] Termos MeSH secundário: Ácido Ascórbico/metabolismo
Catalase/metabolismo
Espaço Extracelular/metabolismo
Glutationa/metabolismo
Pelargonium/efeitos da radiação
Peroxidases/metabolismo
Fotossíntese/efeitos dos fármacos
Fotossíntese/efeitos da radiação
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/enzimologia
Folhas de Planta/efeitos da radiação
Estômatos de Plantas/efeitos dos fármacos
Estômatos de Plantas/fisiologia
Estômatos de Plantas/efeitos da radiação
Feixe Vascular de Plantas/efeitos dos fármacos
Feixe Vascular de Plantas/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.- (Peroxidases); EC 1.11.1.6 (Catalase); GAN16C9B8O (Glutathione); PLG39H7695 (Paraquat); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE


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[PMID]:27664178
[Au] Autor:Grewe F; Zhu A; Mower JP
[Ad] Endereço:Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska.
[Ti] Título:Loss of a Trans-Splicing nad1 Intron from Geraniaceae and Transfer of the Maturase Gene matR to the Nucleus in Pelargonium.
[So] Source:Genome Biol Evol;8(10):3193-3201, 2016 Oct 30.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mitochondrial nad1 gene of seed plants has a complex structure, including four introns in cis or trans configurations and a maturase gene (matR) hosted within the final intron. In the geranium family (Geraniaceae), however, sequencing of representative species revealed that three of the four introns, including one in a trans configuration and another that hosts matR, were lost from the nad1 gene in their common ancestor. Despite the loss of the host intron, matR has been retained as a freestanding gene in most genera of the family, indicating that this maturase has additional functions beyond the splicing of its host intron. In the common ancestor of Pelargonium, matR was transferred to the nuclear genome, where it was split into two unlinked genes that encode either its reverse transcriptase or maturase domain. Both nuclear genes are transcribed and contain predicted mitochondrial targeting signals, suggesting that they express functional proteins that are imported into mitochondria. The nuclear localization and split domain structure of matR in the Pelargonium nuclear genome offers a unique opportunity to assess the function of these two domains using transgenic approaches.
[Mh] Termos MeSH primário: Endorribonucleases/genética
Evolução Molecular
Transferência Genética Horizontal
NADH Desidrogenase/genética
Nucleotidiltransferases/genética
Pelargonium/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Núcleo Celular/metabolismo
Íntrons
Deleção de Sequência
Trans-Splicing
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Plant Proteins); EC 1.6.99.3 (NADH Dehydrogenase); EC 2.7.7.- (Nucleotidyltransferases); EC 2.7.7.- (mRNA maturase); EC 3.1.- (Endoribonucleases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27544324
[Au] Autor:Giongo JL; de Almeida Vaucher R; Fausto VP; Quatrin PM; Lopes LQS; Santos RCV; Gündel A; Gomes P; Steppe M
[Ad] Endereço:Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Tecnologia Farmacêutica, Universidade Regional Integrada do Alto Uruguai (URI), Santiago, Rio Grande do Sul, Brazil.
[Ti] Título:Anti-Candida activity assessment of Pelargonium graveolens oil free and nanoemulsion in biofilm formation in hospital medical supplies.
[So] Source:Microb Pathog;100:170-178, 2016 Nov.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infections due to microbial biofilm formation on the surface of catheters and other medical devices are constantly reported as a major cause of morbidity and mortality in patients admitted to hospitals. Furthermore, sessile cells are more resistant to phagocytosis and most antimicrobial, which complicates the treatment of such infections. Researches aimed at new antimicrobial originating mainly from plants have increased in recent years and the development of new strategies for their release is critical in combating the formation of biofilms. Geranium oil (GO) has proven antimicrobial activity. Because of this, the aim of this study was to develop nanoemulsions containing this oil (NEG) and evaluate its activity after the biofilm formation of Candida albicans, Candida tropicalis, Candida glabrata, and Candida krusei in hospital medical supplies. For quantification of the biofilm, crystal violet, total protein, and ATP-bioluminescence assays were used. The results revealed that GO and NEG showed lower MIC for C. albicans and C. tropicalis. The biofilms formed by different species of Candida on the surfaces of polyethylene and polyurethane were quantified. GO and NEG significantly inhibited the formation of biofilms in all species tested on the surfaces of polyethylene. However, NEG antibiofilm has had better activity than GO for C. albicans, C. tropicalis and C. glabrata, according to the surface potential analysis by atomic force microscopy (AFM). The analysis of the biofilm formation on the polyethylene surface by ATP-bioluminescence and CFU showed similar results. In both methods the formation of biofilm in the catheter occurred in greater quantity for C. albicans and C. tropicalis. GO did not significantly inhibit the formation of biofilms only in C. krusei, although NEG significantly increased this activity GO in all species tested when compared to the control training biofilm. The following study shows that the development of NEG may become an effective alternative to reduce the adhesion of microorganisms and prevent infections resulting from the use of some hospital medical materials.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Biofilmes/efeitos dos fármacos
Candida/efeitos dos fármacos
Equipamentos e Provisões/microbiologia
Pelargonium/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/isolamento & purificação
Candida/fisiologia
Emulsões/farmacologia
Hospitais
Testes de Sensibilidade Microbiana
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Emulsions); 0 (Plant Extracts)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171008
[Lr] Data última revisão:
171008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160822
[St] Status:MEDLINE


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[PMID]:27130640
[Au] Autor:Kozan E; Küpeli Akkol E; Süntar I
[Ad] Endereço:Department of Parasitology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Turkey.
[Ti] Título:Potential anthelmintic activity of Pelargonium endlicherianum Fenzl.
[So] Source:J Ethnopharmacol;187:183-6, 2016 Jul 01.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: The decoction prepared from the roots of Pelargonium endlicherianum Fenzl. has been used for the treatment of gastrointestinal parasitism in small ruminants in Turkish Folk Medicine. AIM OF THE STUDY: The aim of the present study is to investigate in vitro anthelmintic activity of the extracts prepared from the roots of Pelargonium endlicherianum Fenzl. (Geraniaceae). MATERIALS AND METHODS: So as to determine the potential anthelmintic effect of the roots of the plant, n-hexane, ethyl acetate (EtOAc) and methanol (MeOH) extracts were successively prepared. In vitro test methods were used for the determination of the anthelmintic effect of the extracts on eggs, larvae and adults of Haemonchus contortus. The extracts were prepared in three increasing concentrations by using Phosphate Buffered Saline (PBS) for egg hatch, larval development assay and adult motility inhibition assay. PBS was used as negative control, levamisole (in PBS) was used as a reference. RESULTS: The extracts exerted significant anthelmintic activity on three lifecycle stages of Haemonchus contortus when compared to the negative control group (P<0.05). The activity was proportional to the concentrations of the plant extracts for egg hatching and the first stage larvae but not for the adult worms. Moreover, the results have shown that the MeOH extract was found to have higher ovicidal and larvicidal effects than the other extracts. CONCLUSIONS: Results of the present research have revealed that MeOH extract obtained from P. endlicherianum demonstrated in vitro anthelmintic effect against the eggs, the first stage larvae and the adult stage of H. contortus. These results confirmed the folkloric use of the plant. It was suggested that the tannin content of the plant could be responsible for the anthelmintic activity.
[Mh] Termos MeSH primário: Anti-Helmínticos/toxicidade
Haemonchus/efeitos dos fármacos
Pelargonium
Extratos Vegetais/toxicidade
[Mh] Termos MeSH secundário: Animais
Feminino
Haemonchus/crescimento & desenvolvimento
Larva/efeitos dos fármacos
Larva/crescimento & desenvolvimento
Dose Letal Mediana
Raízes de Plantas
Zigoto/efeitos dos fármacos
Zigoto/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE



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