Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.503 [Categoria DeCS]
Referências encontradas : 15 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2 ir para página        

  1 / 15 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28599241
[Au] Autor:De Freitas L; Jimenez D; Pimentel S; Mitaine-Offer AC; Pouységu L; Quideau S; Paululat T; Gonzalez-Mujica F; Rojas LB; Rodríguez M; Lacaille-Dubois MA
[Ad] Endereço:Laboratoire de Pharmacognosie, PEPITE EA 4267, UFR des Sciences de Santé, Université de Bourgogne Franche-Comté, 7, Bd. Jeanne d'Arc, BP 87900, F-21079 Dijon Cedex, France; Laboratorio de Productos Naturales, Escuela de Química, Facultad de Ciencias, Universidad Central de Venezuela, Caracas 47102,
[Ti] Título:Triterpene saponins from Billia rosea.
[So] Source:Phytochemistry;141:105-113, 2017 Sep.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Five previously undescribed triterpene saponins, billiosides A-E, and a known analogue, were isolated from the seeds of Billia rosea (Planch. & Linden) C. Ulloa & P. Jørg. Their structures were elucidated on the basis of extensive 1D and 2D NMR experiments ( H, C, DEPT, COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC) and mass spectrometry as (3ß,21ß,22α)-3-[(2-O-ß-D-glucopyranosyl-O-[α-L-arabinopyranosyl-(1 â†’ 4)]-ß-D-glucopyranosyl)oxy]-21-[((2E,6S)-2,6-dimethyl-6-hydroxyocta-2,7-dienoyl)oxy]-22-(acetyloxy)-24-hydroxyolean-12-en-28-oic acid, (3ß,21ß,22α)-3-[(2-O-ß-D-galactopyranosyl-ß-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O-α-L-arabinopyranosyl-(1 â†’ 4)-ß-D-glucopyranoside, (3ß,21ß,22α)-3-[(2-O-ß-D-galactopyranosyl-O-[α-L-arabinopyranosyl-(1 â†’ 4)]-ß-D-xylopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O-ß-D-glucopyranoside, (3ß,21ß,22α)-3-[(2-O-ß-D-galactopyranosyl-O-[α-L-arabinopyranosyl-(1 â†’ 4)]-ß-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O-ß-D-glucopyranoside, (3ß,21ß,22α)-3-[(2-O-ß-D-galactopyranosyl-O-[α-L-arabinopyranosyl-(1 â†’ 4)]-ß-D-glucopyranosyl)oxy]-21,22-dihydroxyolean-12-en-28-yl O-ß-D-glucopyranosyl-(1 â†’ 6)-ß-D-glucopyranoside, and dipteroside A. Billiosides B and C exhibited moderate effects when tested as hepatic glucose-6-phosphatase inhibitors and as glucose intestinal absorption inhibitors, using in situ rat intestinal segments.
[Mh] Termos MeSH primário: Hippocastanaceae/química
Intestinos/efeitos dos fármacos
Saponinas/farmacologia
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Glucose/metabolismo
Hipoglicemiantes/isolamento & purificação
Hipoglicemiantes/farmacologia
Absorção Intestinal/efeitos dos fármacos
Microssomos Hepáticos/efeitos dos fármacos
Estrutura Molecular
Ratos
Saponinas/isolamento & purificação
Sementes/química
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Saponins); 0 (Triterpenes); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE


  2 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27268715
[Au] Autor:Harris AJ; Fu C; Xiang QY; Holland L; Wen J
[Ad] Endereço:Oklahoma State University, Department of Botany, 301 Physical Sciences, Stillwater, OK 74078, USA. Electronic address: aj.harris@inbox.com.
[Ti] Título:Testing the monophyly of Aesculus L. and Billia Peyr., woody genera of tribe Hippocastaneae of the Sapindaceae.
[So] Source:Mol Phylogenet Evol;102:145-51, 2016 Sep.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hippocastaneae is a well-supported clade in Sapindaceae that comprises 15+ species; 12+ in Aesculus, two in Billia, and one in Handeliodendron Rehder. The monophyly of Aesculus and Billia were widely assumed, but a recent molecular phylogenetic study of Sapindanceae used seven species of Aesculus and one each of Billia and Handeliodendron and showed that Billia and Handeliodendron were nested within Aesculus. Here, we tested the hypothesis that Aesculus and Billia are mutually monophyletic using phylogenetic analyses of seven molecular markers and 31 accessions of Hippocastaneae representing 14 species. We performed phylogenetic analyses using a dataset of concatenated genes as well as with coalescent method for constructing a species tree from individual gene trees. The analysis of seven concatenated markers and the species tree strongly supported the mutual monophyly of Aesculus and Billia. We also recovered support for the traditional arrangement of genera within Hippocastaneae: Aesculus and Billia comprising a clade that is sister to Handeliodendron. However, the relationships among the genera remain incompletely resolved.
[Mh] Termos MeSH primário: Aesculus/genética
Hippocastanaceae/genética
[Mh] Termos MeSH secundário: Aesculus/classificação
Cloroplastos/genética
DNA de Plantas/química
DNA de Plantas/isolamento & purificação
DNA de Plantas/metabolismo
Hippocastanaceae/classificação
Dados de Sequência Molecular
Filogenia
Folhas de Planta/genética
Sapindaceae/classificação
Sapindaceae/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


  3 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27261572
[Au] Autor:Zhang H; Luo X; Ke J; Duan Y; He Y; Zhang D; Cai M; Sun G; Sun X
[Ad] Endereço:School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zhanghh@mail.ujs.edu.cn.
[Ti] Título:Procyanidins, from Castanea mollissima Bl. shell, induces autophagy following apoptosis associated with PI3K/AKT/mTOR inhibition in HepG2 cells.
[So] Source:Biomed Pharmacother;81:15-24, 2016 Jul.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Procyanidins from Castanea mollissima Bl. shell (CSPCs) induced autophagy and apoptosis in HepG2 cells and its mechanism remains to be examined. In this paper, autophagy was measured by the lipid modification of light chain-3 (LC3) and the formation of autophagosomes. Hoechst 33258 staining and flow cytometer analysis were used to measure apoptosis. The western blot analysis was used to examine the effects of CSPCs on the expression of LC3, PI3K, phosphorylation of AKT, mTOR, Bcl-2, Bad, Bax, BID and cleaved caspase 3 in HepG2 cells. The results showed that 3-methyladenine (3-MA) and apoptosis inhibitor (Z-VAD) could inhibited the death of HepG2 induced by CSPCs for 48h (150µg/mL). CSPCs induced the accumulation of autophagosomes and microtubule-associated proteins light chain 3-II (LC3-II, a marker of autophagy). P-AKT, PI3K and mTOR were significantly decreased on CSPCs exposure. However, these phenomena were not observed in the group pretreated with the autophagy inhibitor 3-MA and Z-VAD. CSPCs also induced the expression of Bad, Bax and Beclin-1 proteins and decreased the expression of Bcl-2, which was inhibited by 3-MA and Z-VAD. Moreover the apoptotic cell death could be inhibited by 3-MA. In addition, inhibition of LC3-II by siRNA-dependent knockdown attenuated the cleavage of caspase 3. These results suggested CSPCs could trigger autophagy via inhibition of the PI3K/AKT/mTOR signaling pathway, enhanced apoptosis in HepG2 cells which may be associated with the mitochondria-dependent signaling way.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Biflavonoides/farmacologia
Catequina/farmacologia
Hippocastanaceae/química
Fosfatidilinositol 3-Quinases/metabolismo
Proantocianidinas/farmacologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Serina-Treonina Quinases TOR/metabolismo
[Mh] Termos MeSH secundário: Adenina/análogos & derivados
Adenina/farmacologia
Proliferação Celular/efeitos dos fármacos
Proteínas de Fluorescência Verde/metabolismo
Células Hep G2
Seres Humanos
Proteínas de Neoplasias/metabolismo
Oligopeptídeos/farmacologia
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biflavonoids); 0 (Neoplasm Proteins); 0 (Oligopeptides); 0 (Proanthocyanidins); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (benzyloxycarbonyl-valyl-alanyl-aspartic acid); 147336-22-9 (Green Fluorescent Proteins); 4852-22-6 (procyanidin); 5142-23-4 (3-methyladenine); 8R1V1STN48 (Catechin); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); JAC85A2161 (Adenine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160605
[St] Status:MEDLINE


  4 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26829530
[Au] Autor:Montiano MG; Díaz-Faes E; Barriocanal C
[Ad] Endereço:Instituto Nacional del Carbón, INCAR-CSIC, Apartado 73, 33080 Oviedo, Spain.
[Ti] Título:Kinetics of co-pyrolysis of sawdust, coal and tar.
[So] Source:Bioresour Technol;205:222-9, 2016 Apr.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two coals, sawdust and coal tar were selected to prepare briquettes. Thermogravimetric analyses at three heating rates (i.e. 10, 20 and 30°C/min) and up to 1000°C were carried out with the briquette components. Four blends were prepared and the experimental decomposition profiles were compared with the calculated data taking into account the amount of each component in the blend. No interaction was found when comparing the experimental and calculated decomposition profiles of the blends. Isoconversional models OFW (Ozawa-Flynn-Wall) and KAS (Kissinger-Akahira-Sunose) were used to obtain the activation energies of the blend components. The activation energies obtained were introduced in the Coats-Redfern (CR) model to derive the pre-exponential factors. The thermal decomposition profiles calculated using the kinetic parameters were in good agreement with the experimental results in the case of the briquette components, but worse results were obtained in the case of the blends due to their greater complexity.
[Mh] Termos MeSH primário: Carvão Mineral
Madeira/química
[Mh] Termos MeSH secundário: Calefação
Hippocastanaceae/química
Cinética
Modelos Químicos
Termogravimetria
Resíduos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coal); 0 (Waste Products)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE


  5 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26119891
[Au] Autor:Jung BS; Lee NK; Na DS; Yu HH; Paik HD
[Ad] Endereço:Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 143-701, Republic of Korea.
[Ti] Título:Comparative analysis of the antioxidant and anticancer activities of chestnut inner shell extracts prepared with various solvents.
[So] Source:J Sci Food Agric;96(6):2097-102, 2016 04.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The chestnut inner shell (CIS) has long been used as a medicinal herb for strengthening the antioxidant in the cosmetic industry. However, little is known about the antioxidant and anticancer effects of the CIS. RESULTS: The antioxidant and anticancer effects of CIS extracts (CISEs) were investigated by the use of various methods and cancer cell lines, respectively. The total polyphenol content of CISEs using ethanol, methanol, butanol, ethyl acetate and water were 53.30, 43.98, 32.16, 26.79 and 11.53 mg gallic acid equivalents g(-1), respectively. The CISEs using ethanol and methanol exhibited high antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, ß-carotene bleaching and ferric reducing ability of plasma assays; the effects were equivalent to those of butylated hydroxytoluene. All CISEs at 2.5 mg mL(-1) were shown to have a cytotoxic effect over 50%, and the CISE using ethyl acetate at 0.6 mg mL(-1) was proved to have 90% cytotoxic effect against the tested cancer cells. CONCLUSION: The ethanol and methanol CISEs had potent antioxidant effects, and the ethyl acetate CISE had the highest cytotoxicity. These results suggest that CISEs could be used as functional ingredients for antioxidant and anticancer effects in foods as extraction solvents.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/farmacologia
Hippocastanaceae/química
Extratos Vegetais/farmacologia
Sementes/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antioxidantes/química
Compostos de Bifenilo/química
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Ácido Elágico/química
Ácido Gálico/química
Seres Humanos
Fenóis/química
Picratos/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); 19YRN3ZS9P (Ellagic Acid); 632XD903SP (Gallic Acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150630
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7324


  6 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:24306533
[Au] Autor:Merino I; Contreras A; Jing ZP; Gallardo F; Cánovas FM; Gómez L
[Ad] Endereço:Center for Plant Biotechnology and Genomics, Campus de Montegancedo, Universidad Politecnica de Madrid, 28223 Pozuelo de Alarcon, Madrid, Spain.
[Ti] Título:Plantation forestry under global warming: hybrid poplars with improved thermotolerance provide new insights on the in vivo function of small heat shock protein chaperones.
[So] Source:Plant Physiol;164(2):978-91, 2014 Feb.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Climate-driven heat stress is a key factor affecting forest plantation yields. While its effects are expected to worsen during this century, breeding more tolerant genotypes has proven elusive. We report here a substantial and durable increase in the thermotolerance of hybrid poplar (Populus tremula×Populus alba) through overexpression of a major small heat shock protein (sHSP) with convenient features. Experimental evidence was obtained linking protective effects in the transgenic events with the unique chaperone activity of sHSPs. In addition, significant positive correlations were observed between phenotype strength and heterologous sHSP accumulation. The remarkable baseline levels of transgene product (up to 1.8% of total leaf protein) have not been reported in analogous studies with herbaceous species. As judged by protein analyses, such an accumulation is not matched either by endogenous sHSPs in both heat-stressed poplar plants and field-grown adult trees. Quantitative real time-polymerase chain reaction analyses supported these observations and allowed us to identify the poplar members most responsive to heat stress. Interestingly, sHSP overaccumulation was not associated with pleiotropic effects that might decrease yields. The poplar lines developed here also outperformed controls under in vitro and ex vitro culture conditions (callus biomass, shoot production, and ex vitro survival), even in the absence of thermal stress. These results reinforce the feasibility of improving valuable genotypes for plantation forestry, a field where in vitro recalcitrance, long breeding cycles, and other practical factors constrain conventional genetic approaches. They also provide new insights into the biological functions of the least understood family of heat shock protein chaperones.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Agricultura Florestal
Aquecimento Global
Proteínas de Choque Térmico Pequenas/metabolismo
Temperatura Alta
Hibridização Genética
Populus/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Aminobutiratos/farmacologia
Estabilidade Enzimática/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Proteínas de Choque Térmico Pequenas/genética
Resposta ao Choque Térmico/efeitos dos fármacos
Resposta ao Choque Térmico/genética
Hippocastanaceae/efeitos dos fármacos
Hippocastanaceae/metabolismo
Hibridização Genética/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/genética
Folhas de Planta/fisiologia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
Populus/efeitos dos fármacos
Populus/genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminobutyrates); 0 (Heat-Shock Proteins, Small); 0 (Plant Proteins); 51276-47-2 (phosphinothricin)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131206
[St] Status:MEDLINE
[do] DOI:10.1104/pp.113.225730


  7 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:22229950
[Au] Autor:Moreno-Cortés A; Hernández-Verdeja T; Sánchez-Jiménez P; González-Melendi P; Aragoncillo C; Allona I
[Ad] Endereço:Centro de Biotecnología y Genómica de Plantas UPM/INIA, Departamento de Biotecnología, E. T. S. Ingenieros de Montes, Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de Alarcón, E-28223 Madrid, Spain.
[Ti] Título:CsRAV1 induces sylleptic branching in hybrid poplar.
[So] Source:New Phytol;194(1):83-90, 2012 Apr.
[Is] ISSN:1469-8137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:• Sylleptic branching in trees may increase significantly branch number, leaf area and the general growth of the tree, particularly in its early years. Although this is a very important trait, so far little is known about the genes that control this process. • This article characterizes the Castanea sativa RAV1 gene, homologous to Arabidopsis TEM genes, by analyzing its circadian behavior and examining its winter expression in chestnut stems and buds. Transgenic hybrid poplars over-expressing CsRAV1 or showing RNA interference down-regulated PtaRAV1 and PtaRAV2 expression were produced and analyzed. • Over-expression of the CsRAV1 gene induces the early formation of sylleptic branches in hybrid poplar plantlets during the same growing season in which the lateral buds form. Only minor growth differences and no changes in wood anatomy are produced. • The possibility of generating trees with a greater biomass by manipulating the CsRAV1 gene makes CsRAV1 transgenic plants promising candidates for bioenergy production.
[Mh] Termos MeSH primário: Genes de Plantas/genética
Hippocastanaceae/genética
Hibridização Genética
Morfogênese/genética
Populus/crescimento & desenvolvimento
Populus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/genética
Ritmo Circadiano/genética
Flores/genética
Regulação da Expressão Gênica de Plantas
Dados de Sequência Molecular
Fenótipo
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Caules de Planta/genética
Plantas Geneticamente Modificadas
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Estações do Ano
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Madeira/anatomia & histologia
Madeira/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:120227
[Lr] Data última revisão:
120227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120111
[St] Status:MEDLINE
[do] DOI:10.1111/j.1469-8137.2011.04023.x


  8 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:21355996
[Au] Autor:Chen MM; Jiang M; Shang J; Lan X; Yang F; Huang J; Nuss DL; Chen B
[Ad] Endereço:Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, The Key Laboratory of Ministry of Education of China for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
[Ti] Título:CYP1, a hypovirus-regulated cyclophilin, is required for virulence in the chestnut blight fungus.
[So] Source:Mol Plant Pathol;12(3):239-46, 2011 Apr.
[Is] ISSN:1364-3703
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cyclophilins are peptidyl-prolyl cis-trans isomerases that are highly conserved throughout eukaryotes and are the cellular target of the immunosuppressive drug cyclosporin A (CsA). We cloned cyp1, a cyclophilin A-encoding gene in the phytopathogenic fungus Cryphonectria parasitica, and showed that this gene was downregulated following infection by a virulence-attenuating hypovirus. The function of cyp1 was further investigated by construction of a cyp1 deletion mutant. Although the wild-type C. parasitica strain EP155 was sensitive to CsA, the Δcyp1 strain was highly tolerant to CsA, indicating that CYP1 was the target of CsA. Deletion of cyp1 resulted in reduced virulence when inoculated to chestnut stems. Transcriptional analysis revealed that deletion of cyp1 also reduced transcript levels for genes encoding key components of the heterotrimeric guanosine triphosphate-binding protein signalling pathway that are essential for sensing environmental cues and are involved in C. parasitica development and virulence.
[Mh] Termos MeSH primário: Ascomicetos/patogenicidade
Ciclofilinas/metabolismo
Proteínas Fúngicas/metabolismo
Hippocastanaceae/microbiologia
Doenças das Plantas/microbiologia
Vírus de RNA/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ascomicetos/virologia
Southern Blotting
Clonagem Molecular
Ciclofilinas/química
Proteínas Fúngicas/química
Deleção de Genes
Regulação Fúngica da Expressão Gênica
Técnicas de Inativação de Genes
Proteínas Heterotriméricas de Ligação ao GTP/genética
Proteínas Heterotriméricas de Ligação ao GTP/metabolismo
Dados de Sequência Molecular
Fenótipo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Transdução de Sinais/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (RNA, Messenger); EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins); EC 5.2.1.- (Cyclophilins)
[Em] Mês de entrada:1109
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110302
[St] Status:MEDLINE
[do] DOI:10.1111/j.1364-3703.2010.00665.x


  9 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:21199036
[Au] Autor:Toju H; Fukatsu T
[Ad] Endereço:National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
[Ti] Título:Diversity and infection prevalence of endosymbionts in natural populations of the chestnut weevil: relevance of local climate and host plants.
[So] Source:Mol Ecol;20(4):853-68, 2011 Feb.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many insects are ubiquitously associated with multiple endosymbionts, whose infection patterns often exhibit spatial and temporal variations. How such endosymbiont variations are relevant to local adaptation of the host organisms is of ecological interest. Here, we report a comprehensive survey of endosymbionts in natural populations of the chestnut weevil Curculio sikkimensis, whose larvae are notorious pests of cultivated chestnuts and also infest acorns of various wild oaks. From 968 insects representing 55 localities across the Japanese Archipelago and originating from 10 host plant species, we identified six distinct endosymbiont lineages, namely Curculioniphilus, Sodalis, Serratia, Wolbachia, Rickettsia and Spiroplasma, at different infection frequencies (96.7%, 12.8%, 82.3%, 82.5%, 28.2% and 6.8%, respectively) and with different geographical distribution patterns. Multiple endosymbiont infections were very common; 3.18±0.61 (ranging from 1.74 to 5.50) endosymbionts per insect on average in each of the local populations. Five pairs of endosymbionts (Curculioniphilus-Serratia, Curculioniphilus-Wolbachia, Sodalis-Rickettsia, Wolbachia-Rickettsia and Rickettsia-Spiroplasma) co-infected the same host individuals more frequently than expected, while infections with Serratia and Wolbachia were negatively correlated to each other. Infection frequencies of the endosymbionts were significantly correlated with climatic and ecological factors: for example, higher Sodalis, Wolbachia and Rickettsia infections at localities of higher temperature; lower Wolbachia and Rickettsia infections at localities of greater snowfall; and higher Curculioniphilus, Sodalis, Serratia, Wolbachia and Rickettsia infections on acorns than on chestnuts. These patterns are discussed in relation to potential host-endosymbiont co-evolution via local adaptation across geographical populations.
[Mh] Termos MeSH primário: Clima
Rickettsia/classificação
Spiroplasma/classificação
Simbiose
Gorgulhos/microbiologia
Wolbachia/classificação
[Mh] Termos MeSH secundário: Adaptação Biológica
Animais
DNA Bacteriano/genética
Geografia
Hippocastanaceae
Japão
Filogenia
Quercus
RNA Ribossômico 16S/genética
Rickettsia/fisiologia
Análise de Sequência de DNA
Spiroplasma/fisiologia
Wolbachia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:110202
[Lr] Data última revisão:
110202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110105
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-294X.2010.04980.x


  10 / 15 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:20394151
[Au] Autor:Kraft K
[Ad] Endereço:Lehrstuhl Naturheilkunde, Universität Rostock. karin.kraft@med.uni-rostock.de
[Ti] Título:[Blunt injuries].
[Ti] Título:Stumpfe Verletzungen..
[So] Source:MMW Fortschr Med;152(11):22-3, 2010 Mar 18.
[Is] ISSN:1438-3276
[Cp] País de publicação:Germany
[La] Idioma:ger
[Mh] Termos MeSH primário: Fitoterapia
Ferimentos não Penetrantes/terapia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/uso terapêutico
Arnica
Bandagens
Confrei
Hippocastanaceae
Seres Humanos
Hypericum
Mentha piperita
Pomadas
Extratos Vegetais/uso terapêutico
Preparações de Plantas/uso terapêutico
Ferimentos não Penetrantes/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Ointments); 0 (Plant Extracts); 0 (Plant Preparations)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:100416
[Lr] Data última revisão:
100416
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100417
[St] Status:MEDLINE



página 1 de 2 ir para página        
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde