Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.583.520.362 [Categoria DeCS] Referências encontradas : 291 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28869908 [Au] Autor: Zhang YY; Jiang HY; Liu M; Hu K; Wang WG; Du X; Li XN; Pu JX; Sun HD [Ad] Endereço: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 10039, People's Republic of China; Yunnan Key Laboratory of Natural Med [Ti] Título: Bioactive ent-kaurane diterpenoids from Isodon rubescens. [So] Source: Phytochemistry;143:199-207, 2017 Nov. [Is] ISSN: 1873-3700 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Seven previously undescribed 7,20-epoxy-ent-kaurane diterpenoids, isojiangrubesins A-G, along with seventeen known ones, were isolated from the aerial parts of Isodon rubescens. Their structures were characterized on the basis of spectroscopic methods and signal-crystal X-ray diffraction. All of these compounds were evaluated for their in vitro cytotoxicity against five human tumor cell lines (HL-60, SMMC-7721, A-549, MCF-7, and SW480). Four isolates exhibited significant inhibitory ability against all cell lines, with IC values ranging from 0.5 to 6.5 µM; They also strongly inhibited NO production in LPS-stimulated RAW264.7 cells. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação Antineoplásicos Fitogênicos/farmacologia Diterpenos Caurânicos/isolamento & purificação Diterpenos Caurânicos/farmacologia Medicamentos de Ervas Chinesas/isolamento & purificação Medicamentos de Ervas Chinesas/farmacologia Isodon/química [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Cristalografia por Raios X Diterpenos Caurânicos/química Ensaios de Seleção de Medicamentos Antitumorais Medicamentos de Ervas Chinesas/química Células HL-60 Seres Humanos Lipopolissacarídeos/farmacologia Macrófagos/efeitos dos fármacos Estrutura Molecular Óxido Nítrico/biossíntese Componentes Aéreos da Planta/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Kaurane); 0 (Drugs, Chinese Herbal); 0 (Lipopolysaccharides); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171002 [Lr] Data última revisão: 171002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170905 [St] Status: MEDLINE

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[PMID]: 28758169 [Au] Autor: Liu M; Wang WG; Sun HD; Pu JX [Ad] Endereço: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, P. R. China. pujianxin@mail.kib.ac.cn. [Ti] Título: Diterpenoids from Isodon species: an update. [So] Source: Nat Prod Rep;34(9):1090-1140, 2017 Aug 30. [Is] ISSN: 1460-4752 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Covering: December 2005 to June 2016. Previous review: Nat. Prod. Rep., 2006, 23, 673-698Over the last decade, great efforts have been made to conduct phytochemistry research on the genus Isodon, which have led to the isolation and identification of a number of diterpenoids. At the same time, these newly reported diterpenoids with diverse structures have led to new findings on their biological functions and chemical synthesis research. In this update, we review more than 600 new diterpenoids, including their structures, classifications, biogenetic pathways, bioactivities, and chemical synthesis. [Mh] Termos MeSH primário: Diterpenos Isodon/química [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/isolamento & purificação Antineoplásicos Fitogênicos/farmacologia Diterpenos/química Diterpenos/isolamento & purificação Diterpenos/farmacologia Estrutura Molecular Ressonância Magnética Nuclear Biomolecular [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170801 [St] Status: MEDLINE [do] DOI: 10.1039/c7np00027h

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[PMID]: 28654256 [Au] Autor: Jiang HY; Wang WG; Tang JW; Liu M; Li XR; Hu K; Du X; Li XN; Zhang HB; Pu JX; Sun HD [Ad] Endereço: State Key Laboratory of Phytochemistry and Plant Resources in West China, and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, People's Republic of China. [Ti] Título: Structurally Diverse Diterpenoids from Isodon scoparius and Their Bioactivity. [So] Source: J Nat Prod;80(7):2026-2036, 2017 Jul 28. [Is] ISSN: 1520-6025 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Fourteen new diterpenoids (1-14) based on four skeletal types and two known analogues (15 and 16) were isolated from the aerial parts of Isodon scoparius. Compound 2 is the first ent-kaurane diterpenoid featuring a 1,11-ether bridge, and the structures of these new compounds were established mainly by NMR and MS methods. The absolute configurations of 1 and 5 and the relative configuration of 3 were determined using single-crystal X-ray diffraction. The absolute configuration of 14 was determined by comparison of the experimental and calculated electronic circular dichroism spectra. Compounds 1, 4, and 15 were active against five human tumor cell lines (HL-60, SMMC-7721, A-549, MCF-7, and SW-480), and they also inhibited NO production in LPS-stimulated RAW264.7 cells, with IC values of 1.0, 3.1, and 1.8 µM, respectively. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos Diterpenos Caurânicos Isodon/química Componentes Aéreos da Planta/química [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/isolamento & purificação Antineoplásicos Fitogênicos/farmacologia Cristalografia por Raios X Diterpenos Caurânicos/química Diterpenos Caurânicos/isolamento & purificação Diterpenos Caurânicos/farmacologia Ensaios de Seleção de Medicamentos Antitumorais Medicamentos de Ervas Chinesas/química Medicamentos de Ervas Chinesas/isolamento & purificação Medicamentos de Ervas Chinesas/farmacologia Células HL-60 Seres Humanos Lipopolissacarídeos/farmacologia Macrófagos/efeitos dos fármacos Estrutura Molecular Óxido Nítrico/biossíntese Ressonância Magnética Nuclear Biomolecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Kaurane); 0 (Drugs, Chinese Herbal); 0 (Lipopolysaccharides); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170628 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jnatprod.7b00163

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[PMID]: 28558679 [Au] Autor: Zeb A; Ullah F; Ayaz M; Ahmad S; Sadiq A [Ad] Endereço: Department of Pharmacy, University of Malakand, Chakdara, Dir, KPK, (L)18000, Pakistan. [Ti] Título: Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract. [So] Source: BMC Complement Altern Med;17(1):284, 2017 May 30. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Since long, natural sources have been explored for possible managements of various diseases. In this context, the study is designed to evaluate Isodon rugosus Wall. ex Benth for biological potentials including antibacterial, anthelmintic, insecticidal, anti-termites and anti-Pharaoh activities followed by GC-MS analysis of active fraction to identify various bioactive compounds. METHODS: I. rugosus was investigated against eight bacterial strains using well diffusion method and microdilution method with ceftriaxone as positive control. Similarly, the insecticidal activity was carried out against Tribolium castaneum, Rhyzopertha dominica, Monomorium pharaonis and Heterotermis indicola following contact toxicity method. Likewise, anthelmintic activity was performed against Ascaridia galli and Pherethima posthuma using albendazole as positive control, in which the paralysis and death times of the worms were observed. The GC-MS analysis of the most active solvent fraction was performed for identifications of various bioactive compounds. RESULTS: Among the tested samples of I. rugosus, flavonoids and ethyl acetate fraction exhibited high antibacterial activities. The crude saponins showed highest anthelmintic activity against Pherethima posthuma and Ascaridia galli with death times of 27.67 and 29.22 min respectively at concentrations of 40 mg/ml. In insecticidal activity, chloroform fraction and saponins exhibited notable results against R. dominica (60 and 70%) and T. castaneum (70 and 76%) at concentration of 200 mg/ml. In anti-termite assay, all the plant samples showed overwhelming results, i.e. all the 25 termites were killed on the 3rd day. Similarly, in anti-Pharaoh activity, the chloroform, ethyl acetate and saponins fractions were most potent, each exhibiting LD of <0.1 mg/ml. In GC-MS analysis, total of 57 compounds were identified. Some of the bioactive compounds identified in GC-MS analysis are palmitic acid, hinokiol, α-amyrin, phytol, ethyl linolate, cyclohexanone, hinokione, methyl palmitate, ethyl palmitate and stigmasterol acetate. CONCLUSIONS: Based on our current results, it can be concluded that I. rugosus possess strong antibacterial, insecticidal and anthelmintic potentials having crude saponins and ethyl acetate as the most active fractions. The GC-MS analysis and biological assays reveal that ethyl acetate fraction is a suitable target for the isolation of diverse array of bioactive compounds. [Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia Antibacterianos/farmacologia Inseticidas/farmacologia Isodon/química Extratos Vegetais/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Anti-Helmínticos/química Anti-Helmínticos/isolamento & purificação Antibacterianos/química Antibacterianos/isolamento & purificação Bactérias/efeitos dos fármacos Cromatografia Gasosa-Espectrometria de Massas Helmintos/efeitos dos fármacos Insetos/efeitos dos fármacos Inseticidas/química Inseticidas/isolamento & purificação Extratos Vegetais/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anthelmintics); 0 (Anti-Bacterial Agents); 0 (Insecticides); 0 (Plant Extracts) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170601 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1798-9

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[PMID]: 28445526 [Au] Autor: Pelot KA; Hagelthorn DM; Addison JB; Zerbe P [Ad] Endereço: Department of Plant Biology, University of California-Davis, Davis, California, United States of America. [Ti] Título: Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant Isodon rubescens is catalyzed by a pair of diterpene synthases. [So] Source: PLoS One;12(4):e0176507, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Plants produce an immense diversity of natural products (i.e. secondary or specialized metabolites) that offer a rich source of known and potentially new pharmaceuticals and other desirable bioproducts. The Traditional Chinese Medicinal plant Isodon rubescens (Lamiaceae) contains an array of bioactive labdane-related diterpenoid natural products. Of these, the ent-kauranoid oridonin is the most prominent specialized metabolite that has been extensively studied for its potent antimicrobial and anticancer efficacy. Mining of a previously established transcriptome of I. rubescens leaf tissue identified seven diterpene synthase (diTPSs) candidates. Here we report the functional characterization of four I. rubescens diTPSs. IrTPS5 and IrTPS3 were identified as an ent-copalyl diphosphate (CPP) synthase and a (+)-CPP synthase, respectively. Distinct transcript abundance of IrTPS5 and the predicted ent-CPP synthase IrTPS1 suggested a role of IrTPS5 in specialized ent-kaurene metabolism possibly en route to oridonin. Nicotiana benthamiana co-expression assays demonstrated that IrTPS4 functions sequentially with IrTPS3 to form miltiradiene. In addition, IrTPS2 converted the IrTPS3 product (+)-CPP into the hydroxylated tricyclic diterpene nezukol not previously identified in I. rubescens. Metabolite profiling verified the presence of nezukol in I. rubescens leaf tissue. The proposed IrTPS2-catalyzed reaction mechanism proceeds via the common ionization of the diphosphate group of (+)-CPP, followed by formation of an intermediary pimar-15-en-8-yl+ carbocation and neutralization of the carbocation by water capture at C-8 to yield nezukol, as confirmed by nuclear magnetic resonance (NMR) analysis. Oxygenation activity is rare for the family of class I diTPSs and offers new catalysts for developing metabolic engineering platforms to produce a broader spectrum of bioactive diterpenoid natural products. [Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo Diterpenos/metabolismo Isodon/metabolismo Proteínas de Plantas/metabolismo [Mh] Termos MeSH secundário: Alquil e Aril Transferases/química Alquil e Aril Transferases/classificação Biocatálise Clonagem Molecular Diterpenos/química Diterpenos Caurânicos/biossíntese Diterpenos Caurânicos/química Cromatografia Gasosa-Espectrometria de Massas Expressão Gênica Isodon/química Isodon/genética Espectroscopia de Ressonância Magnética Metaboloma Filogenia Folhas de Planta/química Folhas de Planta/metabolismo Proteínas de Plantas/química Proteínas de Plantas/classificação Plantas Medicinais/química Plantas Medicinais/metabolismo Tabaco/química Tabaco/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Diterpenes); 0 (Diterpenes, Kaurane); 0 (Plant Proteins); 0 (nezukol); EC 2.5.- (Alkyl and Aryl Transferases) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170907 [Lr] Data última revisão: 170907 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170427 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0176507

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[PMID]: 28411855 [Au] Autor: Pi J; Jin H; Jiang J; Yang F; Cai H; Yang P; Cai J; Chen ZW [Ad] Endereço: State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau 999078, China; Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago 60612, USA. [Ti] Título: Single molecule force spectroscopy for in-situ probing oridonin inhibited ROS-mediated EGF-EGFR interactions in living KYSE-150 cells. [So] Source: Pharmacol Res;119:479-489, 2017 May. [Is] ISSN: 1096-1186 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: As the active anticancer component of Rabdosia Rubescens, oridonin has been proved to show strong anticancer activity in cancer cells, which is also found to be closely related to its specific inhibition effects on the EGFR tyrosine kinase activity. In this study, atomic force microscopy based single molecule force spectroscopy (AFM-SMFS) was used for real-time and in-situ detection of EGF-EGFR interactions in living esophageal cancer KYSE-150 cells to evaluate the anticancer activity of oridonin for the first time. Oridonin was found to induce apoptosis and also reduce EGFR expression in KYSE-150 cells. AFM-SMFS results demonstrated that oridonin could inhibit the binding between EGF and EGFR in KYSE-150 cells by decreasing the unbinding force and binding probability for EGF-EGFR complexes, which was further proved to be closely associated with the intracellular ROS level. More precise mechanism studies based on AFM-SMFS demonstrated that oridonin treatment could decrease the energy barrier width, increase the dissociation off rate constant and decrease the activation energy of EGF-EGFR complexes in ROS dependent way, suggesting oridonin as a strong anticancer agent targeting EGF-EGFR interactions in cancer cells through ROS dependent mechanism. Our results not only suggested oridonin as a strong anticancer agent targeting EGF-EGFR interactions in ROS dependent mechanism, but also highlighted AFM-SMFS as a powerful technique for pharmacodynamic studies by detecting ligand-receptor interactions, which was also expected to be developed into a promising tool for the screening and mechanism studies of drugs. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Diterpenos Caurânicos/farmacologia Fator de Crescimento Epidérmico/metabolismo Neoplasias Esofágicas/tratamento farmacológico Espécies Reativas de Oxigênio/metabolismo Receptor do Fator de Crescimento Epidérmico/metabolismo [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Apoptose/efeitos dos fármacos Linhagem Celular Tumoral Diterpenos Caurânicos/química Neoplasias Esofágicas/metabolismo Esôfago/efeitos dos fármacos Esôfago/metabolismo Seres Humanos Isodon/química Microscopia de Força Atômica [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Kaurane); 0 (Reactive Oxygen Species); 0APJ98UCLQ (oridonin); 62229-50-9 (Epidermal Growth Factor); EC 2.7.10.1 (Receptor, Epidermal Growth Factor) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170619 [Lr] Data última revisão: 170619 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170417 [St] Status: MEDLINE

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[PMID]: 28376864 [Au] Autor: Tian L; Xie K; Sheng D; Wan X; Zhu G [Ad] Endereço: Traditional Chinese medicine pharmacy, Zhejiang Hospital, No. 12 Lingyin Road, Xihu District, Zhejiang, Hangzhou, China. [Ti] Título: Antiangiogenic effects of oridonin. [So] Source: BMC Complement Altern Med;17(1):192, 2017 Apr 04. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Oridonin, the major terpene found in Rabdosia rubescens (Henmsl.) Hara, is widely used as a dietary supplement and therapeutic drug. Oridonin has been proven to possess good anti-tumour activity, but little is known about its effect on angiogenesis. The aim of this study was to investigate the antiangiogenic effects of oridonin in vivo and in vitro and prove that oridonin anti-tumour activity is based on suppressing angiogenesis. METHODS: In vitro, the antiangiogenesis effect was studied by proliferation, apoptosis, migration, invasion, and tube formation experiments on human umbilical vascular endothelial cells (HUVECs). In vivo, using the Tg (fli1: GFP) zebrafish model, the embryonic vasculogenesis and postnatal regeneration were evaluated. The vascular endothelial growth factor (VEGF) signalling pathway gene expressions were assessed by reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the inhibition effects on tumour growth and metastasis were observed using a xenograft zebrafish tumour model and xenograft nude mouse tumour model. Angiogenesis was assayed by immunostaining with cluster of differentiation 31. Importantly, the proteins were identified as being differentially expressed in an in vivo model by two-dimensional electrophoresis-mass spectrometry (2D-MS) and western blot (WB). RESULTS: The results indicated that oridonin inhibited HUVEC proliferation, migration, invasion, and tube formation and induced cell apoptosis. Oridonin inhibited zebrafish angiogenesis during embryonic development and tail fin regeneration. RT-PCR showed that oridonin decreased the VEGFA, VEGFR2, and VEGFR3 expressions in zebrafish, while the TP53 expression increased. Moreover, oridonin had strong effects on tumour growth and metastasis in vivo. 2D-MS identified a total of 50 proteins differentially expressed (17 up-expressed, 28 down-expressed). Lastly, WB showed that Claudin 1, Claudin 4, and Claudin 7 were closely related to tumour growth and metastasis. CONCLUSION: This study demonstrated that oridonin could inhibit tumour growth and metastasis, which mainly based on oridonin antiangiogenic effects. Claudin 1, Claudin 4, and Claudin 7 were the main contributors to the mechanism. [Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia Diterpenos Caurânicos/farmacologia Isodon/química [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/isolamento & purificação Antineoplásicos Fitogênicos/farmacologia Ensaios de Seleção de Medicamentos Antitumorais Feminino Células Endoteliais da Veia Umbilical Humana Seres Humanos Masculino Camundongos Camundongos Nus Peixe-Zebra [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Angiogenesis Inhibitors); 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Kaurane); 0APJ98UCLQ (oridonin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170425 [Lr] Data última revisão: 170425 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170406 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1706-3

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[PMID]: 28300699 [Au] Autor: Liu HC; Xiang ZB; Wang Q; Li BY; Jin YS; Chen HS [Ad] Endereço: School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Department of Pharmacy, Beijing Chao-yang Hospital, Capital Medical University, Beijing 100020, China. [Ti] Título: Monomeric and dimeric ent-kauranoid-type diterpenoids from rabdosia japonica and their cytotoxicity and anti-HBV activities. [So] Source: Fitoterapia;118:94-100, 2017 Apr. [Is] ISSN: 1873-6971 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Two new ent-kauranoid-type diterpenoids (1 and 2) and one new rare dimer of ent-kauranoids (3) with a cyclobutane ring by a [2+2] cycloaddition, together with nine known diterpenoids (4-12) were obtained from the aerial parts of Rabdosia japonica. Their chemical structures were established by 1D and 2D NMR techniques and mass spectrometry and by comparison with spectroscopic data reported. All ent-kauranoids were test for their cytotoxic effects against A549, HCT116, CCRF-CEM and HL-60 tumor cell lines. Compounds 1, 2, 4, 5, 7, 10 and 12 showed potent and selective cytotoxicity. In addition, some selected ent-kauranoids were test for their anti-HBV activities, and the results showed compound 8 had inhibitory effect on HBsAg with a 59% inhibition ratio at the concentration of 20µg/mL. [Mh] Termos MeSH primário: Antivirais/química Diterpenos Caurânicos/química Isodon/química [Mh] Termos MeSH secundário: Antivirais/isolamento & purificação Linhagem Celular Tumoral Diterpenos Caurânicos/isolamento & purificação Ensaios de Seleção de Medicamentos Antitumorais Células HL-60 Vírus da Hepatite B/efeitos dos fármacos Seres Humanos Espectroscopia de Ressonância Magnética Espectrometria de Massas Estrutura Molecular Componentes Aéreos da Planta/química Extratos Vegetais/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Diterpenes, Kaurane); 0 (Plant Extracts) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170425 [Lr] Data última revisão: 170425 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170317 [St] Status: MEDLINE

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[PMID]: 28259901 [Au] Autor: Zhao J; Zhang M; He P; Zhao J; Chen Y; Qi J; Wang Y [Ad] Endereço: Department of Hematology, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China. [Ti] Título: Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells. [So] Source: Mol Med Rep;15(4):1807-1815, 2017 Apr. [Is] ISSN: 1791-3004 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: Oridonin is a diterpenoid compound isolated from the medicinal herb Rabdosia rubescens, and has shown marked antitumor effects against different types of cancer. However, the definitive systematic molecular mechanism underlying the antitumor activity of oridonin in multiple myeloma remains to be elucidated. In the present study, cell viability and cytotoxicity were examined to determine the appropriate concentration for proteomic investigation. In addition, cell apoptosis was evaluated using flow cytometry and transmission electron microscopy. A proteomic investigation using a two­dimensional electrophoresis system and mass spectrometry was performed to identify and characterize the global proteome of the apoptosis induced by oridonin. Of the proteins identiï¬�ed, seven were involved in the anticancer effects of oridonin. Regulation of the expression and function of target proteins, stathmin, dihydrofolate reductase and pyruvate dehydrogenase E1ß, may be potential, therapeutic strategies to effectively treat multiple myeloma. These ï¬�ndings provide novel information on the molecular mechanisms underlying the anticancer properties of oridonin in multiple myeloma. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Apoptose/efeitos dos fármacos Sobrevivência Celular/efeitos dos fármacos Diterpenos Caurânicos/farmacologia Mieloma Múltiplo/tratamento farmacológico [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química Linhagem Celular Tumoral Diterpenos Caurânicos/química Seres Humanos Isodon/química Mieloma Múltiplo/metabolismo Mieloma Múltiplo/patologia Proteoma/metabolismo Proteômica [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Kaurane); 0 (Proteome); 0APJ98UCLQ (oridonin) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170512 [Lr] Data última revisão: 170512 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170306 [St] Status: MEDLINE [do] DOI: 10.3892/mmr.2017.6213

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[PMID]: 28218684 [Au] Autor: Wan J; Jiang HY; Tang JW; Li XR; Du X; Li Y; Sun HD; Pu JX [Ad] Endereço: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. wanjun@imm.ac.cn. [Ti] Título: Ent-Abietanoids Isolated from Isodon serra. [So] Source: Molecules;22(2), 2017 Feb 17. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Four new -abietane diterpenoids, along with four known ones were isolated from the aerial parts of , a traditional Chinese folk medicine. The new diterpenoids were named as serrin K ( ), xerophilusin XVII ( ), and enanderianins Q and R ( and ), while the known ones were identified as rubescansin J ( ), (3α,14ß)-3,18-[(1-methylethane-1,1-diyl)dioxy]- -abieta-7,15(17)-diene-14,16-diol ( ), xerophilusin XIV ( ), and enanderianin P ( ), respectively. Their structures were elucidated by extensive spectroscopic analysis and comparison with the literature. Compound showed remarkable inhibitory activity towards NO production in LPS-stimulated RAW264.7 cells (IC = 1.8 µM) and weak cytotoxicity towards five human tumor cell lines (HL-60, SMMC-7721, A-549, MCF-7, SW480). [Mh] Termos MeSH primário: Diterpenos Abietanos/química Diterpenos Abietanos/isolamento & purificação Isodon/química Extratos Vegetais/química Extratos Vegetais/isolamento & purificação [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/farmacologia Linhagem Celular Linhagem Celular Tumoral Diterpenos Abietanos/farmacologia Seres Humanos Macrófagos/efeitos dos fármacos Macrófagos/metabolismo Espectroscopia de Ressonância Magnética Camundongos Modelos Moleculares Conformação Molecular Estrutura Molecular Óxido Nítrico/biossíntese Componentes Aéreos da Planta/química Extratos Vegetais/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Diterpenes, Abietane); 0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170509 [Lr] Data última revisão: 170509 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170221 [St] Status: MEDLINE

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