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Pesquisa : B01.650.940.800.575.912.250.583.520.647 [Categoria DeCS]
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[PMID]:28362991
[Au] Autor:Mohr FB; Lermen C; Gazim ZC; Gonçalves JE; Alberton O
[Ad] Endereço:Programa de Pós-Graduação em Biotecnologia Aplicada à Agricultura, Universidade Paranaense, Umuarama, PR, Brasil.
[Ti] Título:Antifungal activity, yield, and composition of Ocimum gratissimum essential oil.
[So] Source:Genet Mol Res;16(1), 2017 Mar 16.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Ocimum gratissimum L. or clove basil, belongs to the Lamiaceae family, has various desirable uses and applications. Beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. This study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (EO) of O. gratissimum. The species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. The EO was obtained by hydrodistillation of dried leaves in a modified Clevenger apparatus, followed by determination of its content. Chemical characterization was carried out by gas chromatography-mass spectrometry (GC-MS). Microbial activity was assessed using the broth microdilution method, by determining the minimum inhibitory concentration (MIC), in order to compare the antimicrobial effect of EO in 10 isolates-Fusarium oxysporum f. sp tracheiphilum (CMM-0033), F. oxysporum f. sp. cubense (CMM-0813 and CMM-2819), F. oxysporum f. sp lycopersici (CMM-1104), F. solani (CMM-3828), Rhizoctonia solani (CMM-3274), and Macrophomina phaseolina (CMM-2715, CMM-3875, CMM-3615, and CMM-3650). The EO was a highly effective inhibitor of the studied phytopathogenic fungi, with MICs varying from 31.25 to 125 µg/mL. F. oxysporum f. sp lycopersici and R. solani were the most sensitive; both were inhibited at an MIC of 31.25 µg/mL. The EO content in the plant extract was 0.18%. Thirty chemical compounds were detected via GC-MS, with linalool (32.9%) being the major compound followed by 1,8-cineole (21.9%), both oxygenated monoterpenes. It can be concluded that clove basil EO is a highly effective antifungal agent, and therefore, a potential alternative for the control of plant pathogenic diseases.
[Mh] Termos MeSH primário: Ascomicetos/efeitos dos fármacos
Fungos Mitospóricos/efeitos dos fármacos
Ocimum/química
Óleos Voláteis/química
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/farmacologia
Ascomicetos/classificação
Fusarium/classificação
Fusarium/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Testes de Sensibilidade Microbiana
Fungos Mitospóricos/classificação
Folhas de Planta/química
Óleos Vegetais/química
Óleos Vegetais/farmacologia
Rhizoctonia/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Oils, Volatile); 0 (Plant Oils)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16019542


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[PMID]:28103929
[Au] Autor:Avetisyan A; Markosian A; Petrosyan M; Sahakyan N; Babayan A; Aloyan S; Trchounian A
[Ad] Endereço:Nairian CJSC, Khorenatsi 15, Yerevan, Armenia.
[Ti] Título:Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars.
[So] Source:BMC Complement Altern Med;17(1):60, 2017 Jan 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The plants belonging to the Ocimum genus of the Lamiaceae family are considered to be a rich source of essential oils which have expressed biological activity and use in different area of human activity. There is a great variety of chemotypes within the same basil species. Essential oils from three different cultivars of basil, O. basilicum var. purpureum, O. basilicum var. thyrsiflora, and O. citriodorum Vis. were the subjects of our investigations. METHODS: The oils were obtained by steam distillation in a Clevenger-type apparatus. The gas chromatography mass selective analysis was used to determine their chemical composition. The antioxidant activities of these essential oils were measured using 1,1-diphenyl-2-picrylhydrazyl assays; the tyrosinase inhibition abilities of the given group of oils were also assessed spectophotometrically, and the antimicrobial activity of the essential oils was determined by the agar diffusion method, minimal inhibitory concentrations were expressed. RESULTS: According to the results, the qualitative and quantitative composition of essential oils was quite different: O. basilicum var. purpureum essential oil contained 57.3% methyl-chavicol (estragol); O. basilicum var. thyrsiflora oil had 68.0% linalool. The main constituents of O. citriodorum oil were nerol (23.0%) and citral (20.7%). The highest antioxidant activity was demonstrated by O. basilicum var. thyrsiflora essential oil. This oil has also exhibited the highest tyrosinase inhibition level, whereas the oil from O. citriodorum cultivar demonstrated the highest antimicrobial activity. CONCLUSIONS: The results obtained indicate that these essential oils have antioxidant, antibacterial and antifungal activity and can be used as natural antioxidant and antimicrobial agents in medicine, food industry and cosmetics.
[Mh] Termos MeSH primário: Ocimum/química
Óleos Voláteis/farmacologia
Óleos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Colorimetria
Depuradores de Radicais Livres/farmacologia
Monofenol Mono-Oxigenase/antagonistas & inibidores
Óleos Voláteis/química
Óleos Vegetais/química
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Free Radical Scavengers); 0 (Oils, Volatile); 0 (Plant Oils); EC 1.14.18.1 (Monophenol Monooxygenase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1587-5


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[PMID]:27914825
[Au] Autor:Singh V; Krishan P; Singh N; Kumar A; Shri R
[Ad] Endereço:Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, 147002 India.
[Ti] Título:Amelioration of ischemia-reperfusion induced functional and biochemical deficit in mice by Ocimum kilimandscharicum leaf extract.
[So] Source:Biomed Pharmacother;85:556-563, 2017 Jan.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The genus Ocimum (family Lamiaceae) has been revered for its diverse biological activities. Various species have been used traditionally to treat CNS disorders and are proven to have neuroprotective effect that is often attributed to their significant antioxidant activity. Ocimum kilimandscharicum (Karpoora Thulasi), a prominent member of this genus is reported to have marked antioxidant activity but its neuroprotective potential has not been explored. Thus, present study was designed to evaluate the cerebroprotective effect of O. kilimandscharicum leaf extract (OKLE) in mice against ischemia reperfusion (I-R) induced brain injury. Bilateral common carotid artery occlusion (BCCAO) for 15min followed by 24h reperfusion was used to induce brain damage in Swiss Albino mice. Animals were treated with OKLE (200 and 400mg/kg, po) once daily for 7days after I-R. Morris water maze and elevated plus maze tests were used to assess long and short term memory while neurological severity score was used to determine motor coordination. Histopathological evaluation (TTC staining) along with brain biochemical parameters (TBARS, reduced GSH and SOD activity) were determined to outline neuroprotective mechanism of OKLE. I-R resulted in marked cognitive impairments, motor incoordination in mice, significant brain damage and increased oxidative stress. Treatment with OKLE produced functional recovery in mice which is manifested by improved memory and motor coordination; reduced cerebral infarct size and brain oxidative stress (TBARS levels) and elevated endogenous antioxidants (reduced GSH and SOD activity). In addition, OKLE showed DPPH radical scavenging and reducing power in-vitro. These results show that O. kilimandscharicum mitigated the neurodegenerative changed induced by I-R in mice probably due to its antioxidant activity.
[Mh] Termos MeSH primário: Ocimum/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
Traumatismo por Reperfusão/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/farmacologia
Infarto Cerebral/etiologia
Infarto Cerebral/patologia
Relação Dose-Resposta a Droga
Aprendizagem em Labirinto
Memória/efeitos dos fármacos
Camundongos
Fitoterapia
Extratos Vegetais/química
Extratos Vegetais/toxicidade
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27982173
[Au] Autor:Pimenta MS; Lobo NS; Vieira VC; Costa ÂM; Costa FN; Pereira SL
[Ad] Endereço:Dental School, UNIFOR - University of Fortaleza, Fortaleza, CE, Brazil.
[Ti] Título:Effect of Ocimum gratissimum in Mouthrinses on De Novo Plaque Formation. A Randomized Clinical Trial..
[So] Source:Braz Dent J;27(6):646-651, 2016 Oct-Dec.
[Is] ISSN:1806-4760
[Cp] País de publicação:Brazil
[La] Idioma:por
[Ab] Resumo:The aim of this study was to evaluate the antiplaque effect of Ocimum gratissimum (Og) by in vivo investigation. Fifteen healthy volunteers participated in a crossover, double-blind clinical study, using a 3-day partial-mouth plaque accumulation model. The participants abolished any method of mechanical oral hygiene and they were randomly assigned to initially use just the following mouthrinses: distilled water (DW solution), 0.12% chlorhexidine digluconate (CLX solution) or 10% Og (Og solution). The plaque index (PLI) was recorded in all mandibular teeth at the end of the trial and the Kruskal-Wallis (α=0.05) and Mann-Whitney (α=0.05) tests were used to estimate the difference among groups. The clinical results showed statistically significant difference among the groups (p<0.05), favoring the CLX solution and Og solution, but the first was more effective (p<0.05). The mouthrinses containing 0.12% chlorhexidine digluconate and 10% Og were able to inhibit plaque re-growth, however Og showed more limited results in comparison to CLX.
[Mh] Termos MeSH primário: Placa Dentária/prevenção & controle
Antissépticos Bucais
Ocimum
[Mh] Termos MeSH secundário: Adolescente
Adulto
Clorexidina/administração & dosagem
Clorexidina/análogos & derivados
Estudos Cross-Over
Índice de Placa Dentária
Método Duplo-Cego
Feminino
Seres Humanos
Masculino
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Mouthwashes); MOR84MUD8E (chlorhexidine gluconate); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27877073
[Au] Autor:Chao PY; Lin JA; Ting WJ; Lee HH; Hsieh K; Chiu YW; Lai TJ; Hwang JM; Liu JR; Huang CY
[Ad] Endereço:Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
[Ti] Título: aqueous extract reduces plasma lipid in hypercholesterol-fed hamsters.
[So] Source:Int J Med Sci;13(11):819-824, 2016.
[Is] ISSN:1449-1907
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Hyperlipidemia is a significant risk factor in the development of atherosclerosis and related diseases which are major health problem in many developed and developing countries that can lead to fatality due to the changes in lifestyle and dietary habits in this modern age. In the present study, the aqueous extract (OGE) was tested for the lowering effect on the serum lipid level of male hamsters on a high-fat (12%) and high-cholesterol (0.2%) diet (HFCD). The results showed that the levels of serum high-density-lipoprotein-cholesterol (HDL-C) low-density-lipoprotein-cholesterol (LDL-C), total cholesterol (TC), and triglycerols (TG) were increased in the HFCD group (113±11, 259±87, 629±175 and 625±262, respectively), as compared to the control normal diet group (51±8, 19±5, 77±16 and 101±44, respectively). When co-treated with various doses (10 and 20 mg/kg) of the OGE or rosuvastatin, the rats exhibited the restoration of normal serum LDL-C, TC, and TG levels. Therefore, we suggest that the aqueous extract may have the potential function of lowering serum lipid in rats.
[Mh] Termos MeSH primário: Anticolesterolemiantes/uso terapêutico
HDL-Colesterol/efeitos dos fármacos
LDL-Colesterol/efeitos dos fármacos
Hiperlipidemias/tratamento farmacológico
Ocimum/química
Extratos Vegetais/uso terapêutico
Triglicerídeos/sangue
[Mh] Termos MeSH secundário: Animais
Anticolesterolemiantes/administração & dosagem
HDL-Colesterol/sangue
LDL-Colesterol/sangue
Cricetinae
Dieta Hiperlipídica/efeitos adversos
Modelos Animais de Doenças
Hiperlipidemias/sangue
Fígado/patologia
Masculino
Mesocricetus
Extratos Vegetais/administração & dosagem
Ratos
Rosuvastatina Cálcica/administração & dosagem
Rosuvastatina Cálcica/uso terapêutico
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticholesteremic Agents); 0 (Cholesterol, HDL); 0 (Cholesterol, LDL); 0 (Plant Extracts); 0 (Triglycerides); 059QF0KO0R (Water); 83MVU38M7Q (Rosuvastatin Calcium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


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[PMID]:27725240
[Au] Autor:Singh V; Kahol A; Singh IP; Saraf I; Shri R
[Ad] Endereço:Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India.
[Ti] Título:Evaluation of anti-amnesic effect of extracts of selected Ocimum species using in-vitro and in-vivo models.
[So] Source:J Ethnopharmacol;193:490-499, 2016 Dec 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Ocimum species are traditionally used for the treatment of anxiety, nerve pain, convulsions and a variety of neurodegenerative disorders. The present study was undertaken to evaluate the anti-amnesic effect of O. basilicum L., O. sanctum L. and O. gratissimum L. extracts using in-vitro and in-vivo models. MATERIALS AND METHODS: In-vitro acetylcholinesterase (AChE) inhibitory and antioxidant activities of hydro-methanol extracts of plants were evaluated using Ellman and DPPH and FRAP assays, respectively. The most active extract i.e. O. basilicum extract (OBE) was further explored for the possible anti-amnesic activity in mouse model of scopolamine induced amnesia using behavioral models (elevated plus maze and passive shock avoidance task). Brain AChE activity, oxidative profile and histopathological studies were assessed to outline the anti-amnesic mechanism of the extract. RESULTS: Significant antioxidant and AChE inhibition activity was observed with all prepared extracts and however, OBE showed most marked free radical scavenging, reducing power and AChE inhibition (IC 0.65±0.15mg/ml) activity. Basil leaves were standardized with respect to content of 7 phenolic acids using a HPLC-PDA method. A TLC densitometric method was employed to determine the quercetin content in the leaves. The in-vivo studies showed that OBE pre-treatment (200 and 400mg/kg, p.o.) reversed the memory deficit induced by scopolamine in mice, evident by significant (p<0.05) decrease in the transfer latency time and increase in step down latency in elevated plus maze and passive shock avoidance task, respectively. Moreover, OBE significantly reduced the brain AChE activity and oxidative stress. Further, histopathological examination of brain tissues displayed decrease in vacuolated cytoplasm and increase in pyramidal cells in hippocampal and cortical regions with OBE pre-treatment. CONCLUSION: OBE possesses antioxidant and AChE inhibitory activity. These biochemical changes are responsible for the anti-amnesic and neuroprotective activities of O. basilicum which may be attributed to the presence of phenolic and flavonoid compounds. This can be developed as an effective anti-amnesic drug.
[Mh] Termos MeSH primário: Amnésia/tratamento farmacológico
Comportamento Animal/efeitos dos fármacos
Córtex Cerebral/efeitos dos fármacos
Hipocampo/efeitos dos fármacos
Memória/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Ocimum basilicum/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Amnésia/induzido quimicamente
Amnésia/enzimologia
Amnésia/psicologia
Animais
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Aprendizagem da Esquiva/efeitos dos fármacos
Compostos de Bifenilo/química
Córtex Cerebral/enzimologia
Córtex Cerebral/patologia
Córtex Cerebral/fisiopatologia
Inibidores da Colinesterase/isolamento & purificação
Inibidores da Colinesterase/farmacologia
Cromatografia Líquida de Alta Pressão
Cognição/efeitos dos fármacos
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Feminino
Proteínas Ligadas por GPI/antagonistas & inibidores
Proteínas Ligadas por GPI/metabolismo
Hipocampo/enzimologia
Hipocampo/patologia
Hipocampo/fisiopatologia
Masculino
Aprendizagem em Labirinto/efeitos dos fármacos
Metanol/química
Camundongos Endogâmicos BALB C
Fármacos Neuroprotetores/química
Fármacos Neuroprotetores/isolamento & purificação
Ocimum/química
Ocimum sanctum/química
Fenóis/isolamento & purificação
Fenóis/farmacologia
Fitoterapia
Picratos/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Plantas Medicinais
Tempo de Reação
Hidrobrometo de Escopolamina
Solventes/química
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Cholinesterase Inhibitors); 0 (GPI-Linked Proteins); 0 (Neuroprotective Agents); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); 0 (Solvents); 451IFR0GXB (Scopolamine Hydrobromide); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); EC 3.1.1.7 (Acetylcholinesterase); EC 3.1.1.7 (Ache protein, mouse); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


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[PMID]:27519164
[Au] Autor:Anand A; Jayaramaiah RH; Beedkar SD; Singh PA; Joshi RS; Mulani FA; Dholakia BB; Punekar SA; Gade WN; Thulasiram HV; Giri AP
[Ad] Endereço:Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411008, Maharashtra, India; Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pune 411008, Maharashtra, India.
[Ti] Título:Comparative functional characterization of eugenol synthase from four different Ocimum species: Implications on eugenol accumulation.
[So] Source:Biochim Biophys Acta;1864(11):1539-47, 2016 11.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus. Though enzymes from phenylpropanoid pathway have been characterized from few plants, limited information exists on how they modulate levels of secondary metabolites. Here, we performed phenylpropanoid profiling in different tissues from five Ocimum species, which revealed significant variations in secondary metabolites including eugenol, eugenol methyl ether, estragole and methyl cinnamate levels. Expression analysis of eugenol synthase (EGS) gene showed higher transcript levels especially in young leaves and inflorescence; and were positively correlated with eugenol contents. Additionally, transcript levels of coniferyl alcohol acyl transferase, a key enzyme diverting pool of substrate to phenylpropanoids, were in accordance with their abundance in respective species. In particular, eugenol methyl transferase expression positively correlated with higher levels of eugenol methyl ether in Ocimum tenuiflorum. Further, EGSs were functionally characterized from four Ocimum species varying in their eugenol contents. Kinetic and expression analyses indicated, higher enzyme turnover and transcripts levels, in species accumulating more eugenol. Moreover, biochemical and bioinformatics studies demonstrated that coniferyl acetate was the preferred substrate over coumaryl acetate when used, individually or together, in the enzyme assay. Overall, this study revealed the preliminary evidence for varied accumulation of eugenol and its abundance over chavicol in these Ocimum species. Current findings could potentially provide novel insights for metabolic modulations in medicinal and aromatic plants.
[Mh] Termos MeSH primário: Eugenol/metabolismo
Regulação da Expressão Gênica de Plantas
Ocimum/enzimologia
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo
[Mh] Termos MeSH secundário: Compostos Alílicos/isolamento & purificação
Compostos Alílicos/metabolismo
Sequência de Aminoácidos
Anisóis/isolamento & purificação
Anisóis/metabolismo
Cinamatos/isolamento & purificação
Cinamatos/metabolismo
Sequência Conservada
Ensaios Enzimáticos
Eugenol/análogos & derivados
Eugenol/isolamento & purificação
Metiltransferases/genética
Metiltransferases/metabolismo
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Ocimum/genética
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/química
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética
Fenóis/isolamento & purificação
Fenóis/metabolismo
Filogenia
Folhas de Planta/enzimologia
Folhas de Planta/genética
Óleos Vegetais/química
Proteínas/genética
Proteínas/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Metabolismo Secundário
Alinhamento de Sequência
Especificidade por Substrato
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4-allylphenol); 0 (Allyl Compounds); 0 (Anisoles); 0 (Cinnamates); 0 (Phenols); 0 (Plant Oils); 0 (Proteins); 0 (RNA, Messenger); 29T9VA6R7M (methyleugenol); 3T8H1794QW (Eugenol); 533CV2ZCQL (methyl cinnamate); 9NIW07V3ET (estragole); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.1.- (eugenol synthase, Ocimum basilium); EC 2.1.1.- (Methyltransferases); EC 2.1.1.- (eugenol O-methyltransferase); EC 2.3.1.84 (alcohol O-acetyltransferase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


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[PMID]:27508961
[Au] Autor:Farag MA; Ezzat SM; Salama MM; Tadros MG; Serya RA
[Ti] Título:Anti-acetylcholinesterase activity of essential oils and their major constituents from four Ocimum species.
[So] Source:Z Naturforsch C;71(11-12):393-402, 2016 Nov 01.
[Is] ISSN:0939-5075
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Ocimum is a genus of considerable importance in traditional medicine worldwide. The goal of this study was to examine the anti-acetylcholinesterase activity of Ocimum essential oils and to correlate the activity with their chemical profiles using a metabolome based GC-MS approach coupled to chemometrics. Further, molecular docking was adopted to rationalize the activity of some essential oil isolates. Essential oil prepared from the four species O. basilicum, O. africanum, O. americanum, and O. minimum exhibited significant anti-acetylcholinesterase activity with (IC50 0.22, 0.175, 0.57 and 0.152 mg/mL, respectively) comparable to that of physostigmine (IC50 0.27 mg/mL). The phenylpropanoids (i.e. estragole) constituted the most dominant chemical group in O. basilicum (sweet basil) and O. minimum, whereas camphor (a ketone) was the most abundant in O. africanum and O. americanum. Supervised and unsupervised multivariate data analyses clearly separated O. africanum and O. americanum from other accessions, with estragole, camphor and, to less extent, ß-linalool contributing to species segregation. Estragole was found the most active AchE inhibitor (IC50 0.337 µM) followed by cineole (IC50 2.27 µM), camphor (IC50 21.43 µM) and eugenol (IC50 40.32 µM). Molecular docking revealed that these compounds bind to key amino acids in the catalytic domain of AchE, similar to standard drugs.
[Mh] Termos MeSH primário: Acetilcolinesterase/efeitos dos fármacos
Inibidores da Colinesterase/farmacologia
Ocimum/química
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Concentração Inibidora 50
Simulação de Acoplamento Molecular
Análise Multivariada
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Oils, Volatile); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE


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[PMID]:27417228
[Au] Autor:Sutili FJ; Velasquez A; Pinheiro CG; Heinzmann BM; Gatlin DM; Baldisserotto B
[Ad] Endereço:CAPES Foundation, Ministry of Education of Brazil, Brasília, DF, 70040-020, Brazil; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil.
[Ti] Título:Evaluation of Ocimum americanum essential oil as an additive in red drum (Sciaenops ocellatus) diets.
[So] Source:Fish Shellfish Immunol;56:155-161, 2016 Sep.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study evaluated productive parameters, whole-body composition, non-specific immune responses and pH and microbiota of digestive tract contents of red drum (Sciaenops ocellatus) fed diets supplemented with Ocimum americanum essential oil (OAEO) (0 - control, 0.25, 0.5, 1.0 and 2.0 g/kg diet). After 7 weeks no significant differences in productive parameters and whole-body composition were observed. Plasma and intestinal lysozyme measurements and pH of the stomach and intestine (6 h after feeding) did not show significant differences among groups. Intestinal microbial community in fish fed the basal and OAEO diets (all concentrations) were identical. However, red drum fed the diet with OAEO at 1.0 g/kg had significantly increased intraperitoneal fat deposition and stomach pH (2 h after feeding) and decreased superoxide ion production (NBT-test) compared to the control group. Hemolytic activity of the complement system increased in fish fed diets containing OAEO. Red blood cells from fish fed the lowest OAEO concentration (0.25 g/kg) showed significant lower fragility in erythrocyte osmotic fragility assay, but fish fed 0.5 and 1.0 g/kg showed significant higher erythrocyte fragility. Lysozyme measurement in the supernatant of stomach content was significantly higher in fish fed the diet supplemented at 0.5 g/kg. Based on these various results, OAEO at different supplementation levels did not influence growth performance and intestinal microbial community; however, the EO added to the diet showed effects on immunological responses of red drum.
[Mh] Termos MeSH primário: Dieta/veterinária
Suplementos Nutricionais
Imunidade Inata
Ocimum/química
Óleos Voláteis
Perciformes/fisiologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Relação Dose-Resposta a Droga
Microbioma Gastrointestinal
Trato Gastrointestinal/química
Trato Gastrointestinal/microbiologia
Concentração de Íons de Hidrogênio
Perciformes/imunologia
Perciformes/microbiologia
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE


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[PMID]:27270753
[Au] Autor:Sutili FJ; Murari AL; Silva LL; Gressler LT; Heinzmann BM; de Vargas AC; Schmidt D; Baldisserotto B
[Ad] Endereço:Department of Physiology and Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
[Ti] Título:The use of Ocimum americanum essential oil against the pathogens Aeromonas hydrophila and Gyrodactylus sp. in silver catfish (Rhamdia quelen).
[So] Source:Lett Appl Microbiol;63(2):82-8, 2016 Aug.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The bactericidal activity (minimum inhibitory concentration (MIC)-test) of Ocimum americanum (inflorescences) essential oil (OAEO) against Aeromonas hydrophila was determined in this study. Also investigated was the potential of OAEO and the main compound found in the oil (linalool) at subinhibitory concentrations to be inhibitors of haemolysis caused by Aer. hydrophila in fish erythrocytes. An in vivo experiment was conducted to evaluate the survival of fish (Rhamdia quelen) experimentally infected with Aer. hydrophila and exposed to OAEO. A second experiment was conducted to evaluate the in vitro and in vivo activity of OAEO (mix from inflorescences and leaves) against the parasite Gyrodactylus sp. The OAEO showed weak in vitro activity against Aer. hydrophila (6400 µg ml(-1) ). Subinhibitory concentrations of OAEO (100 µg ml(-1) ) inhibited haemolysis (90%) caused by Aer. hydrophila in fish erythrocytes, however, linalool did not inhibit haemolysis activity. At the low concentrations (10 and 20 mg l(-1) ) added to the water, OAEO promoted the survival of fish experimentally infected with Aer. hydrophila. Lastly, the OAEO mix (50 mg l(-1) ) was effective against Gyrodactylus sp., significantly reducing (60%) the number of parasites in the fish. SIGNIFICANCE AND IMPACT OF THE STUDY: Phytochemicals, such as essential oils (EOs) are a great source of new molecules and have shown potential to be used in aquaculture systems. However, additional studies focused on the in vivo efficacy, mode of action and identification of the active compounds are needed. This study determined the potential of Ocimum americanum EO for use against two important fish pathogens, Aeromonas hydrophila and Gyrodactylus sp., as well as providing preliminary information about the role of the main EO compound (linalool) against Aer. hydrophila virulence.
[Mh] Termos MeSH primário: Aeromonas hydrophila/efeitos dos fármacos
Peixes-Gato/microbiologia
Peixes-Gato/parasitologia
Infecções por Cestoides/veterinária
Doenças dos Peixes/tratamento farmacológico
Infecções por Bactérias Gram-Negativas/veterinária
Monoterpenos/farmacologia
Óleos Voláteis/farmacologia
Platelmintos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Aquicultura
Infecções por Cestoides/tratamento farmacológico
Eritrócitos/microbiologia
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Hemólise/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Ocimum/química
Compostos Fitoquímicos/farmacologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Oils, Volatile); 0 (Phytochemicals); D81QY6I88E (linalool)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12602



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