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[PMID]:29024882
[Au] Autor:Ko JA; Furuta N; Lim HB
[Ad] Endereço:Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do 448-701, South Korea; Future Environmental Research Center, Korea Institute of Toxicology, Jinju 660-844, South Korea.
[Ti] Título:Quantitative mapping of elements in basil leaves (Ocimum basilicum) based on cesium concentration and growth period using laser ablation ICP-MS.
[So] Source:Chemosphere;190:368-374, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Quantitative elemental mapping of metallic pollutants in sweet basil was studied by laser ablation (LA)-ICP-MS. For this, the sweet basil was cultivated in Hoagland nutrient solution spiked with 100 and 1000 ng mL of Cs for 10-60 days. Then, the Cs distribution in collected leaves was determined by LA-ICP-MS using lab-synthesized standard pellets based on NIST 1573a tomato leaves. For comparison, S, Ca, and K were also simultaneously determined in this measurement with a C signal from the leaves as an internal standard. The obtained calibration curves showed linear coefficient of determination (R ) of 0.991 for K and 0.999 for Cs. The concentration of Cs measured in the basil leaves increased with growth period and pollutant concentration, and accumulation followed the order of leaf margin, petiole, midrib, and veins. Although no visible symptom was detected, significant suppression of the growth rate was observed due to the presence of high-concentration Cs. The experimental model demonstrated herein showed potential for studying the influence of radioactive pollutants on plants and other organisms in the food chain.
[Mh] Termos MeSH primário: Ocimum basilicum/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Césio/análise
Césio/farmacocinética
Césio/farmacologia
Radioisótopos de Césio/farmacologia
Elementos
Alimentos
Espectrometria de Massas/métodos
Ocimum basilicum/efeitos dos fármacos
Ocimum basilicum/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cesium Radioisotopes); 0 (Elements); 1KSV9V4Y4I (Cesium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE


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[PMID]:28922359
[Au] Autor:Pyne R; Honig J; Vaiciunas J; Koroch A; Wyenandt C; Bonos S; Simon J
[Ad] Endereço:Department of Plant Biology, Rutgers University, New Brunswick, New Jersey, United States of America.
[Ti] Título:A first linkage map and downy mildew resistance QTL discovery for sweet basil (Ocimum basilicum) facilitated by double digestion restriction site associated DNA sequencing (ddRADseq).
[So] Source:PLoS One;12(9):e0184319, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Limited understanding of sweet basil (Ocimum basilicum L.) genetics and genome structure has reduced efficiency of breeding strategies. This is evidenced by the rapid, worldwide dissemination of basil downy mildew (Peronospora belbahrii) in the absence of resistant cultivars. In an effort to improve available genetic resources, expressed sequence tag simple sequence repeat (EST-SSR) and single nucleotide polymorphism (SNP) markers were developed and used to genotype the MRI x SB22 F2 mapping population, which segregates for response to downy mildew. SNP markers were generated from genomic sequences derived from double digestion restriction site associated DNA sequencing (ddRADseq). Disomic segregation was observed in both SNP and EST-SSR markers providing evidence of an O. basilicum allotetraploid genome structure and allowing for subsequent analysis of the mapping population as a diploid intercross. A dense linkage map was constructed using 42 EST-SSR and 1,847 SNP markers spanning 3,030.9 cM. Multiple quantitative trait loci (QTL) model (MQM) analysis identified three QTL that explained 37-55% of phenotypic variance associated with downy mildew response across three environments. A single major QTL, dm11.1 explained 21-28% of phenotypic variance and demonstrated dominant gene action. Two minor QTL dm9.1 and dm14.1 explained 5-16% and 4-18% of phenotypic variance, respectively. Evidence is provided for an additive effect between the two minor QTL and the major QTL dm11.1 increasing downy mildew susceptibility. Results indicate that ddRADseq-facilitated SNP and SSR marker genotyping is an effective approach for mapping the sweet basil genome.
[Mh] Termos MeSH primário: Resistência à Doença/genética
Ligação Genética
Sequenciamento de Nucleotídeos em Larga Escala
Ocimum basilicum/genética
Polimorfismo de Nucleotídeo Único
Característica Quantitativa Herdável
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184319


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[PMID]:28393527
[Au] Autor:Yang T; Zhao B; Kinchla AJ; Clark JM; He L
[Ti] Título:Investigation of Pesticide Penetration and Persistence on Harvested and Live Basil Leaves Using Surface-Enhanced Raman Scattering Mapping.
[So] Source:J Agric Food Chem;65(17):3541-3550, 2017 May 03.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding pesticide behavior in plants is important for effectively applying pesticides and in reducing pesticide exposures from ingestion. This study aimed to investigate the penetration and persistence of pesticides applied on harvested and live basil leaves. Surface-enhanced Raman scattering (SERS) mapping was applied for in situ and real-time tracking of pesticides over time using gold nanoparticles as probes. The results showed that, after surface exposure of 30 min to 48 h, pesticides (10 mg/L) penetrated more rapidly and deeply into the live leaves than the harvested leaves. The systemic pesticide thiabendazole and the nonsystemic pesticide ferbam can penetrate into the live leaves with depths of 225 and 130 µm, respectively, and the harvested leaves with depths of 180 and 18 µm, respectively, after 48 h of exposure. The effects of leaf integrity and age on thiabendazole penetration were also evaluated on live basil leaves after 24 h of exposure. Thiabendazole (10 mg/L) when applied onto intact leaves penetrated deeper (170 µm) than when applied onto damaged leaves (80 µm) prepared with 20 scrapes on the top surface of the leaves. Older leaves with a wet mass of 0.204 ± 0.019 g per leaf (45 days after leaf out) allowed more rapid and deeper penetration of pesticides (depth of 165 µm) than younger leaves with a wet mass of 0.053 ± 0.007 g per leaf (15 days after leaf out, depth of 95 µm). The degradation of thiabendazole on live leaves was detected after 1 week, whereas the apparent degradation of ferbam was detected after 2 weeks. In addition, the removal of pesticides from basil was more efficient when compared with other fresh produce possibly due to the specific gland structure of basil leaves. The information obtained here provides a better understanding of the behavior and biological fate of pesticides on plants.
[Mh] Termos MeSH primário: Ocimum basilicum/química
Resíduos de Praguicidas/análise
Folhas de Planta/química
Análise Espectral Raman/métodos
[Mh] Termos MeSH secundário: Ouro/química
Nanopartículas Metálicas/química
Tiabendazol/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticide Residues); 7440-57-5 (Gold); N1Q45E87DT (Thiabendazole)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00548


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[PMID]:28372241
[Au] Autor:Fratianni F; Cefola M; Pace B; Cozzolino R; De Giulio B; Cozzolino A; d'Acierno A; Coppola R; Logrieco AF; Nazzaro F
[Ad] Endereço:Institute of Food Science, National Council of Research, Via Roma, 64, 83100 Avellino, Italy. Electronic address: fratianni@isa.cnr.it.
[Ti] Título:Changes in visual quality, physiological and biochemical parameters assessed during the postharvest storage at chilling or non-chilling temperatures of three sweet basil (Ocimum basilicum L.) cultivars.
[So] Source:Food Chem;229:752-760, 2017 Aug 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Leaves of three different sweet basil (Ocimum basilicum L.) cultivars (Italico a foglia larga, Cammeo, and Italiano classico) packed in macro-perforated polyethylene bags were stored at chilling (4°C) or non-chilling temperature (12°C) for 9days. During storage, visual quality, physiological (respiration rate, ethylene production, ammonium content) and chemical (antioxidant activity, total polyphenols and polyphenol profile) parameters were measured. Detached leaves stored at chilling temperature showed visual symptoms related to chilling injury, while ethylene production and ammonium content resulted associated to cultivar sensibility to damage at low temperature. Storage at 4°C caused a depletion in polyphenols content and antioxidant capability, which was preserved at 12°C. Regarding the polyphenols profile, stressful storage conditions did not enhance the phenolic metabolism. However, leaves stored at 12°C did not loss a significant amount of metabolites respect to fresh leaves, suggesting the possibility to extend the storability after the expiration date, for a possible recovery of bioactive compounds.
[Mh] Termos MeSH primário: Temperatura Baixa/efeitos adversos
Qualidade dos Alimentos
Armazenamento de Alimentos/métodos
Ocimum basilicum/química
Ocimum basilicum/fisiologia
[Mh] Termos MeSH secundário: Antioxidantes/análise
Armazenamento de Alimentos/normas
Fenóis/análise
Folhas de Planta/química
Folhas de Planta/fisiologia
Polifenóis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Polyphenols)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28366608
[Au] Autor:Abdollahi Mandoulakani B; Eyvazpour E; Ghadimzadeh M
[Ad] Endereço:Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran; Department of Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran. Electronic address: b.abdollahi@urmia.ac.ir.
[Ti] Título:The effect of drought stress on the expression of key genes involved in the biosynthesis of phenylpropanoids and essential oil components in basil (Ocimum basilicum L.).
[So] Source:Phytochemistry;139:1-7, 2017 Jul.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Basil (Ocimum basilicum L.), a medicinal plant of the Lamiaceae family, is used in traditional medicine; its essential oil is a rich source of phenylpropanoids. Methylchavicol and methyleugenol are the most important constituents of basil essential oil. Drought stress is proposed to enhance the essential oil composition and expression levels of the genes involved in its biosynthesis. In the current investigation, an experiment based on a completely randomized design (CRD) with three replications was conducted in the greenhouse to study the effect of drought stress on the expression level of four genes involved in the phenylpropanoid biosynthesis pathway in O. basilicum c.v. Keshkeni luvelou. The genes studied were chavicol O-methyl transferase (CVOMT), eugenol O-methyl transferase (EOMT), cinnamate 4-hydroxylase (C4H), 4-coumarate coA ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD). The effect of drought stress on the essential oil compounds and their relationship with the expression levels of the studied genes were also investigated. Plants were subjected to levels of 100%, 75%, and 50% of field capacity (FC) at the 6-8 leaf stage. Essential oil compounds were identified by gas chromatography/mass spectrometry (GC-MS) at flowering stage and the levels of gene expression were determind by real time PCR in plant leaves at the same stage. Results showed that drought stress increased the amount of methylchavicol, methyleugenol, ß-Myrcene and α-bergamotene. The maximum amount of these compounds was observed at 50% FC. Real-time PCR analysis revealed that severe drought stress (50% FC) increased the expression level of CVOMT and EOMT by about 6.46 and 46.33 times, respectively, whereas those of CAD relatively remained unchanged. The expression level of 4CL and C4H reduced under drought stress conditions. Our results also demonstrated that changes in the expression levels of CVOMT and EOMT are significantly correlated with methylchavicol (r = 0.94, P ≤ 0.05) and methyleugenol (r = 0.98, P ≤ 0.05) content. Thus, drought stress probably increases the methylchavicol and methyleugenol content, in part, through increasing the expression levels of CVOMT and EOMT.
[Mh] Termos MeSH primário: Secas
Ocimum basilicum/química
Óleos Voláteis/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Anisóis/química
Compostos Bicíclicos com Pontes/química
Compostos Bicíclicos com Pontes/isolamento & purificação
Eugenol/análogos & derivados
Eugenol/química
Eugenol/isolamento & purificação
Cromatografia Gasosa-Espectrometria de Massas
Expressão Gênica
Interação Gene-Ambiente
Estrutura Molecular
Monoterpenos/química
Monoterpenos/isolamento & purificação
Ocimum basilicum/genética
Folhas de Planta/química
Óleos Vegetais/química
Plantas Medicinais/genética
Reação em Cadeia da Polimerase em Tempo Real
Sementes/química
Estresse Fisiológico/genética
Transciinamato 4-Mono-Oxigenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anisoles); 0 (Bridged Bicyclo Compounds); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Plant Oils); 123-35-3 (beta-myrcene); 17699-05-7 (alpha-bergamotene); 29T9VA6R7M (methyleugenol); 3T8H1794QW (Eugenol); 9NIW07V3ET (estragole); EC 1.14.13.11 (Trans-Cinnamate 4-Monooxygenase); Z129UMU8LE (basil oil)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


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[PMID]:28317465
[Au] Autor:Cardoso NN; Alviano CS; Blank AF; Arrigoni-Blank MF; Romanos MT; Cunha MM; da Silva AJ; Alviano DS
[Ad] Endereço:a Department of General Microbiology , Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro , Rio de Janeiro , Brazil.
[Ti] Título:Anti-cryptococcal activity of ethanol crude extract and hexane fraction from Ocimum basilicum var. Maria bonita: mechanisms of action and synergism with amphotericin B and Ocimum basilicum essential oil.
[So] Source:Pharm Biol;55(1):1380-1388, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Ocimum basilicum L. (Lamiaceae) has been used in folk medicine to treat headaches, kidney disorders, and intestinal worms. OBJECTIVE: This study evaluates the anti-cryptococcal activity of ethanol crude extract and hexane fraction obtained from O. basilicum var. Maria Bonita leaves. MATERIALS AND METHODS: The MIC values for Cryptococcus sp. were obtained according to Clinical and Laboratory Standards Institute in a range of 0.3-2500 µg/mL. The checkerboard assay evaluated the association of the substances tested (in a range of 0.099-2500 µg/mL) with amphotericin B and O. basilicum essential oil for 48 h. The ethanol extract, hexane fraction and associations in a range of 0.3-2500 µg/mL were tested for pigmentation inhibition after 7 days of treatment. The inhibition of ergosterol synthesis and reduction of capsule size were evaluated after the treatment with ethanol extract (312 µg/mL), hexane fraction (78 µg/mL) and the combinations of essential oil + ethanol extract (78 µg/mL + 19.5 µg/mL, respectively) and essential oil + hexane fraction (39.36 µg/mL + 10 µg/mL, respectively) for 24 and 48 h, respectively. RESULTS: The hexane fraction presented better results than the ethanol extract, with a low MIC (156 µg/mL against C. neoformans T and 312 µg/mL against C. neoformans H99 serotype A and C. gattii WM779 serotype C). The combination of the ethanol extract and hexane fraction with amphotericin B and essential oil enhanced their antifungal activity, reducing the concentration of each substance needed to kill 100% of the inoculum. The substances tested were able to reduce the pigmentation, capsule size and ergosterol synthesis, which suggest they have important mechanisms of action. CONCLUSIONS: These results provide further support for the use of ethanol extracts of O. basilicum as a potential source of antifungal agents.
[Mh] Termos MeSH primário: Anfotericina B/farmacologia
Antifúngicos/farmacologia
Cryptococcus neoformans/efeitos dos fármacos
Etanol/química
Hexanos/química
Ocimum basilicum/química
Extratos Vegetais/farmacologia
Óleos Vegetais/farmacologia
Solventes/química
[Mh] Termos MeSH secundário: Animais
Antifúngicos/isolamento & purificação
Cryptococcus neoformans/crescimento & desenvolvimento
Cryptococcus neoformans/metabolismo
Sinergismo Farmacológico
Ergosterol/biossíntese
Camundongos
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Fitoterapia
Pigmentação/efeitos dos fármacos
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Óleos Vegetais/isolamento & purificação
Plantas Medicinais
Células RAW 264.7
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Hexanes); 0 (Plant Extracts); 0 (Plant Oils); 0 (Solvents); 3K9958V90M (Ethanol); 7XU7A7DROE (Amphotericin B); 91770-38-6 (ocimum oil); Z129UMU8LE (basil oil); Z30RAY509F (Ergosterol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1302483


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[PMID]:28212934
[Au] Autor:Farahmandfar R; Asnaashari M; Salahi MR; Khosravi Rad T
[Ad] Endereço:Department of Food Science and Technology, Sari Agricultural Sciences & Natural Resources University (SANRU), Sari, Iran. Electronic address: r.farahmandfar@sanru.ac.ir.
[Ti] Título:Effects of basil seed gum, Cress seed gum and Quince seed gum on the physical, textural and rheological properties of whipped cream.
[So] Source:Int J Biol Macromol;98:820-828, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study focuses on the physical, textural and rheological properties of low fat (LF) whipped cream with 30% fat content developed with Basil seed gum (BSG), Cress seed gum (CSG) and Quince seed gum (QSG) at the concentration of 0.1 and 0.3% (w/w) and comparison with high fat (HF) whipped cream sample (55%) as control. Flow curves were analyzed using Herschel bulkey and Carreau models through a fitting procedure. The rheological investigations confirmed that all samples were shear thinning fluid exhibiting a yield stress and thixotropy properties. The frequency sweep test showed that at the same gum concentration, mixes containing BSG have higher G', G″ and η than those of mixes with QSG and CSG, and all mixes containing gum displayed weak gel-like behavior. Analysis showed that adding and increasing gums concentration caused to increased viscosity, hardness and overrun, leading to a better quality in the final products. Moreover, textural properties showed that the effect of BSG on hardness and adhesiveness was significantly greater than QSG and CSG at the same concentration. Based on obtained result, 0.3% concentration of added BSG had a much greater effects on the whipped cream properties than those of mixes with QSG and CSG.
[Mh] Termos MeSH primário: Aditivos Alimentares/química
Gomas Vegetais/química
Reologia
[Mh] Termos MeSH secundário: Brassicaceae/química
Aditivos Alimentares/farmacologia
Seres Humanos
Ocimum basilicum/química
Gomas Vegetais/farmacologia
Rosaceae/química
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Food Additives); 0 (Plant Gums)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


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[PMID]:28074741
[Au] Autor:Karoui R; Hassoun A
[Ad] Endereço:Université d'Artois, EA 7394, Institut Charles Viollette, Lens, F-62300, France.
[Ti] Título:Efficiency of Rosemary and Basil Essential Oils on the Shelf-Life Extension of Atlantic Mackerel ( ) Fillets Stored at 2°C.
[So] Source:J AOAC Int;100(2):335-344, 2017 Mar 01.
[Is] ISSN:1060-3271
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of the present study was to evaluate the impact of rosemary and basil essential oils (EOs) on the quality of Atlantic mackerel fillets stored at 2°C up to 15 days. Atlantic mackerel (Scomber scombrus) fillets were periodically evaluated to assess their textural, color, physicochemical, and spectral characteristics. The results indicated that rosemary and basil treatments were effective for inhibiting the formation of total volatile basic nitrogen (TVB-N) and lipid oxidation products during storage. Based on TVB-N values, the shelf life of Atlantic mackerel fillets treated with rosemary and basil EOs was extended by 2 and 5 days, respectively, compared to the control group. Similar results were obtained with thiobarbituric acid-reactive substance analysis, which demonstrated an extended shelf life of Atlantic mackerel immersed with rosemary and basil EOs of 2 and 3 days, respectively, compared to the control group. The factorial discriminant analysis applied on the concatenated first five principal components corresponding to the physicochemical, textural, color, and fluorescence measurements allowed clear discrimination of the three groups, because a correct classification rate of 93.3% was obtained. Therefore, treatment with basil and rosemary EOs, as natural biopreservative compounds, could present a high-potential application in the seafood industry.
[Mh] Termos MeSH primário: Conservantes de Alimentos/química
Armazenamento de Alimentos
Ocimum basilicum/química
Perciformes
Óleos Vegetais/química
Rosmarinus/química
[Mh] Termos MeSH secundário: Aminas/análise
Animais
Colorimetria
Concentração de Íons de Hidrogênio
Lipídeos/química
Malondialdeído/análise
NAD/análise
Oxirredução
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Food Preservatives); 0 (Lipids); 0 (Plant Oils); 0U46U6E8UK (NAD); 4Y8F71G49Q (Malondialdehyde)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.5740/jaoacint.16-0410


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[PMID]:28069370
[Au] Autor:Tan W; Du W; Barrios AC; Armendariz R; Zuverza-Mena N; Ji Z; Chang CH; Zink JI; Hernandez-Viezcas JA; Peralta-Videa JR; Gardea-Torresdey JL
[Ad] Endereço:Environmental Science and Engineering PhD Program, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, United States; University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, 500 W. University Ave., El
[Ti] Título:Surface coating changes the physiological and biochemical impacts of nano-TiO in basil (Ocimum basilicum) plants.
[So] Source:Environ Pollut;222:64-72, 2017 Mar.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Little is known about the effects of surface coating on the interaction of engineered nanoparticles (ENPs) with plants. In this study, basil (Ocimum basilicum) was cultivated for 65 days in soil amended with unmodified, hydrophobic (coated with aluminum oxide and dimethicone), and hydrophilic (coated with aluminum oxide and glycerol) titanium dioxide nanoparticles (nano-TiO ) at 125, 250, 500, and 750 mg nano-TiO kg soil. ICP-OES/MS, SPAD meter, and UV/Vis spectrometry were used to determine Ti and essential elements in tissues, relative chlorophyll content, carbohydrates, and antioxidant response, respectively. Compared with control, hydrophobic and hydrophilic nano-TiO significantly reduced seed germination by 41% and 59%, respectively, while unmodified and hydrophobic nano-TiO significantly decreased shoot biomass by 31% and 37%, respectively (p ≤ 0.05). Roots exposed to hydrophobic particles at 750 mg kg had 87% and 40% more Ti than the pristine and hydrophilic nano-TiO ; however, no differences were found in shoots. The three types of particles affected the homeostasis of essential elements: at 500 mg kg , unmodified particles increased Cu (104%) and Fe (90%); hydrophilic increased Fe (90%); while hydrophobic increased Mn (339%) but reduced Ca (71%), Cu (58%), and P (40%). However, only hydrophobic particles significantly reduced root elongation by 53%. Unmodified, hydrophobic, and hydrophilic particles significantly reduced total sugar by 39%, 38%, and 66%, respectively, compared with control. Moreover, unmodified particles significantly decreased reducing sugar (34%), while hydrophobic particles significantly reduced starch (35%). Although the three particles affected basil plants, coated particles impacted the most its nutritional quality, since they altered more essential elements, starch, and reducing sugars.
[Mh] Termos MeSH primário: Disponibilidade Biológica
Germinação/efeitos dos fármacos
Ocimum basilicum/efeitos dos fármacos
Ocimum basilicum/crescimento & desenvolvimento
Raízes de Plantas/efeitos dos fármacos
Brotos de Planta/efeitos dos fármacos
Titânio/química
[Mh] Termos MeSH secundário: Óxido de Alumínio/química
Biomassa
Dimetilpolisiloxanos/química
Interações Hidrofóbicas e Hidrofílicas/efeitos dos fármacos
Nanopartículas
Raízes de Plantas/crescimento & desenvolvimento
Brotos de Planta/crescimento & desenvolvimento
Solo/química
Titânio/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dimethylpolysiloxanes); 0 (Soil); 0 (dimethicone); D1JT611TNE (Titanium); LMI26O6933 (Aluminum Oxide)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


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[PMID]:28040159
[Au] Autor:Jacobson AP; Wang H; Gill VS; Duvall R; Arce G; Chirtel S; Hammack TS
[Ad] Endereço:U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5001 Campus Dr., College Park, MD 20740, USA. Electronic address: Andrew.Jacobson@fda.hhs.gov.
[Ti] Título:Relative effectiveness of selected preenrichment media for the detection of Salmonella from leafy green produce and herbs.
[So] Source:Food Microbiol;63:123-128, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four buffered preenrichment media (BAX System MP Media (BAX)), Universal Preenrichment Broth (UPB), modified Buffered Peptone Water (mBPW), and Buffered Peptone Water (BPW)) were compared with lactose broth (LB) in the Bacteriological Analytical Manual's (BAM) Salmonella culture method for the analysis of 9 leafy green produce and herb types. Artificially contaminated test portions were pre-enriched in each medium and the results were analyzed statistically using Fisher's Exact 2-tailed F test (p < 0.05) with pairwise comparisons. There was no difference in recovery of Salmonella from curly parsley and basil among the five media (p > 0.05). UPB was consistently among the most effective media for recovery of Salmonella from the nine produce types; however, S. Typhimurium and S. Newport were isolated from cabbage more frequently with mBPW than with UPB (p < 0.05). Comparisons of the results among the preenrichment media from all experimental trials, with leafy green produce and herbs, demonstrate that Salmonella is more effectively detected and isolated using buffered enrichments than with the currently recommended LB (p < 0.05). There were no significant differences among the buffered preenrichments for the detection of Salmonella-positive test portions of the produce tested (BAX (160 Salmonella-positive test portions/480 test portions), UPB (176/480), mBPW (184/480), BPW (169/480), LB (128/480))(p > 0.05).
[Mh] Termos MeSH primário: Meios de Cultura/química
Ocimum basilicum/microbiologia
Petroselinum/microbiologia
Folhas de Planta/microbiologia
Salmonella/isolamento & purificação
Verduras/microbiologia
[Mh] Termos MeSH secundário: Carga Bacteriana
Técnicas Bacteriológicas/métodos
Tampões (Química)
Meios de Cultura/análise
Microbiologia de Alimentos
Lactose/metabolismo
Alface/microbiologia
Salmonella/crescimento & desenvolvimento
Spinacia oleracea/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers); 0 (Culture Media); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE



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