Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.583.640.499 [Categoria DeCS] Referências encontradas : 152 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 29311515 [Au] Autor: Suh WS; Kwon OK; Lee TH; Subedi L; Kim SY; Lee KR [Ad] Endereço: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University. [Ti] Título: Secoiridoid Glycosides from the Twigs of Ligustrum obtusifolium Possess Anti-inflammatory and Neuroprotective Effects. [So] Source: Chem Pharm Bull (Tokyo);66(1):78-83, 2018. [Is] ISSN: 1347-5223 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Two new secoiridoid glycosides, obtusifolisides A and B (1, 2), together with 7 known secoiridoid glycosides (3-9) were isolated from the twigs of Ligustrum obtusifolium. The chemical structures of new compounds were determined by a spectroscopic data analysis, including one and two dimensional (1D-, 2D)-NMR, High resolution-MS, and experiments involving chemical reactions. The isolated secoiridoid glycosides were evaluated for their anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated BV-2 murine microglia cells. Compounds 2, 5, 6, 8, and 9 significantly reduced the production of nitric oxide (NO), with IC values of 5.45, 11.17, 14.62, 15.45, and 14.96 µM, respectively. None of the compounds were toxic to the cells. Additionally, we evaluated the neuroprotective effects of compounds 1-9 on nerve growth factor (NGF) induction in a C6 rat glioma cell line. Compounds 2 and 6 upregulated NGF secretion to 155.56±7.16%, and 139.35±11.65%, respectively, without significant cell toxicity. [Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia Glicosídeos Iridoides/farmacologia Ligustrum/química Fármacos Neuroprotetores/farmacologia Caules de Planta/química [Mh] Termos MeSH secundário: Animais Anti-Inflamatórios não Esteroides/química Anti-Inflamatórios não Esteroides/isolamento & purificação Linhagem Celular Sobrevivência Celular/efeitos dos fármacos Relação Dose-Resposta a Droga Glioma/tratamento farmacológico Glioma/metabolismo Glicosídeos Iridoides/química Glicosídeos Iridoides/isolamento & purificação Lipopolissacarídeos/antagonistas & inibidores Lipopolissacarídeos/farmacologia Camundongos Microglia/efeitos dos fármacos Microglia/metabolismo Conformação Molecular Fatores de Crescimento Neural/antagonistas & inibidores Fatores de Crescimento Neural/metabolismo Fármacos Neuroprotetores/química Fármacos Neuroprotetores/isolamento & purificação Óxido Nítrico/antagonistas & inibidores Óxido Nítrico/biossíntese Ratos Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Iridoid Glycosides); 0 (Lipopolysaccharides); 0 (Nerve Growth Factors); 0 (Neuroprotective Agents); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180213 [Lr] Data última revisão: 180213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180110 [St] Status: MEDLINE [do] DOI: 10.1248/cpb.c17-00720

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[PMID]: 28764781 [Au] Autor: Tang X; Nian H; Li X; Yang Y; Wang X; Xu L; Shi H; Yang X; Liu R [Ad] Endereço: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No.10 Xitoutiao, Youanmenwai, Fengtai District, Beijing, 100069, China. [Ti] Título: Effects of the combined extracts of Herba Epimedii and Fructus Ligustrilucidi on airway remodeling in the asthmatic rats with the treatment of budesonide. [So] Source: BMC Complement Altern Med;17(1):380, 2017 Aug 01. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Asthma is characterized by chronic airway inflammation, leading to structura1 changes in the airway, collectively termed airway remodeling. Airway remodeling is thought to contribute to airway hyper responsiveness and irreversible airflow limitation. The combination of Herba Epimedii (HE) and Fructus Ligustri Lucidi (FLL) decoction and the systemic administration of glucocorticoids (GC) had a synergistic inhibitory action on airway inflammation in the asthmatic model rats. However, the effects of the combination on airway remodeling have not been studied and compared. In the present study, we investigated the effects of the co-administration of combined extracts of HE and FLL with inhaled GC (budesonide) on airway remodeling in the rat asthmatic model induced by ovalbumin (OVA). METHODS: Male Sprague-Dawley rats were sensitized to intraperitoneal OVA followed by repetitive OVA challenge for 7 weeks. Treatments included extracts of HE and FLL (Extracts for short, 100 mg/kg by gastric perfusion), budesonide (1 mg budesonide suspension in 50 ml sterile physiological saline, 3 rats in an ultrasonic nebulizer by nebulized inhabation with a flow of 1.6 ml/min for 30 min), and co-administration of extracts of HE and FLL with budesonide (Co-administration for short) for 4 weeks. Lung histomorphometry and bronchoalveolar lavage fluid (BALF) cell count were assessed 24 h after the final OVA challenge. Levels of interleukin (IL)-4, IL-5 and IgE were measured by ELISA. Expressions of Collagen I and Collagen III were tested by immunohistology. Expressions of transforming growth factor (TGF) -ß1, TGF-ß2 and Smads mRNA were measured by quantitative real-time PCR. RESULTS: Extracts, budesonide and Co-administration significantly reduced allergen-induced increases in the serum levels of IL-4, IL-5 and IgE, the number of eosinophils in BALF, goblet cell hyperplasia, Collagen III integral optical density (IOD) and the mRNA expression of TGF-ß2 and Smad2. Extracts and Co-administration could depress the IOD level of Collagen I and the positive area of Collagen I and Collagen III. Budesonide and Co-administration significantly alleviated the thickening of airway wall. Only Co-administration significantly decreased collagen deposition according to the morphometry of Masson's-stained lung sections, the thickening of airway smooth muscle layer, the number of lymphocytes in BALF and the mRNA expression of TGF-ß1 and Smad3, and this was associated with a significant increase in levels of Smad7 mRNA. CONCLUSIONS: The findings suggested that the combination of budesonide and the herbal extracts had a better synergistic effect on airway remodeling in OVA-reduced asthma rats than the single use of budesonide. [Mh] Termos MeSH primário: Remodelação das Vias Aéreas/efeitos dos fármacos Asma/tratamento farmacológico Budesonida/uso terapêutico Medicamentos de Ervas Chinesas/uso terapêutico Epimedium Ligustrum Pulmão/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Anti-Inflamatórios/farmacologia Anti-Inflamatórios/uso terapêutico Asma/imunologia Asma/metabolismo Asma/patologia Budesonida/farmacologia Colágeno/metabolismo Sinergismo Farmacológico Medicamentos de Ervas Chinesas/farmacologia Imunoglobulina E/sangue Interleucinas/sangue Leucócitos/metabolismo Pulmão/metabolismo Pulmão/patologia Masculino Músculo Liso/patologia Ovalbumina Fitoterapia RNA Mensageiro/metabolismo Ratos Sprague-Dawley Reação em Cadeia da Polimerase em Tempo Real Proteínas Smad/genética Proteínas Smad/metabolismo Fator de Crescimento Transformador beta/genética Fator de Crescimento Transformador beta/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Drugs, Chinese Herbal); 0 (Interleukins); 0 (RNA, Messenger); 0 (Smad Proteins); 0 (Transforming Growth Factor beta); 37341-29-0 (Immunoglobulin E); 51333-22-3 (Budesonide); 9006-59-1 (Ovalbumin); 9007-34-5 (Collagen) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170901 [Lr] Data última revisão: 170901 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170803 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1891-0

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[PMID]: 28618355 [Au] Autor: Ngo QT; Lee HS; Nguyen VT; Kim JA; Woo MH; Min BS [Ad] Endereço: College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk, 38430, Republic of Korea. [Ti] Título: Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation. [So] Source: Phytochemistry;141:147-155, 2017 Sep. [Is] ISSN: 1873-3700 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A previously undescribed nor-dammarane, 3ß,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A-B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC value of 38 ± 2 µM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway. [Mh] Termos MeSH primário: Glicosídeos Iridoides/farmacologia Ligustrum/química Ativação Linfocitária/efeitos dos fármacos Linfócitos T/efeitos dos fármacos Triterpenos/farmacologia [Mh] Termos MeSH secundário: MAP Quinases Reguladas por Sinal Extracelular/metabolismo Frutas/química Seres Humanos Interleucina-2/metabolismo Glicosídeos Iridoides/isolamento & purificação Células Jurkat Proteínas Quinases Ativadas por Mitógeno/metabolismo Estrutura Molecular Fosforilação Triterpenos/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (IL2 protein, human); 0 (Interleukin-2); 0 (Iridoid Glycosides); 0 (Triterpenes); 545-22-2 (dammarane); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinases) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170721 [Lr] Data última revisão: 170721 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170616 [St] Status: MEDLINE

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[PMID]: 28486011 [Au] Autor: Lu Y; Zhou W; Feng Y; Li Y; Liu K; Liu L; Lin D; He Z; Wu X [Ad] Endereço: 1 School of Medicine, Shenzhen University , Shenzhen, P.R. China . [Ti] Título: Acteoside and Acyl-Migrated Acteoside, Compounds in Chinese Kudingcha Tea, Inhibit α-Amylase In Vitro. [So] Source: J Med Food;20(6):577-585, 2017 Jun. [Is] ISSN: 1557-7600 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Acteoside, the predominant polyphenol of small-leaved kudingcha, the Chinese tea, has various biological activities. In this study, we examined the acyl migration of acteoside to isoacteoside with high-temperature treatment of acteoside. The inhibitory effects of acyl-migrated acteoside and acteoside on α-amylase were investigated, as were their binding interaction with α-amylase. The binding of acteoside and isoacteoside to α-amylase was investigated by using the fluorescence spectra assay, circular dichroism, and protein-ligand docking studies. Acteoside was more effective than preheated acteoside and isoacteoside in inhibiting α-amylase activity. Acteoside and isoacteoside binding to α-amylase may induce conformational changes to α-amylase, and the binding site of acteoside and isoacteoside being near the active site pocket of α-amylase may explain the decreased activity of α-amylase. The different affinities and binding sites of acteoside and isoacteoside for α-amylase resulted in different inhibition rates, which may be due to structural differences between acteoside and isoacteoside. [Mh] Termos MeSH primário: Inibidores Enzimáticos/química Glucosídeos/química Ligustrum/química Fenóis/química alfa-Amilases/antagonistas & inibidores [Mh] Termos MeSH secundário: Cinética Folhas de Planta/química Preparações de Plantas Chá/química alfa-Amilases/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzyme Inhibitors); 0 (Glucosides); 0 (Phenols); 0 (Plant Preparations); 0 (Tea); 3TGX09BD5B (acteoside); EC 3.2.1.1 (alpha-Amylases) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170822 [Lr] Data última revisão: 170822 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170510 [St] Status: MEDLINE [do] DOI: 10.1089/jmf.2016.3910

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[PMID]: 28445407 [Au] Autor: Li H; Yao W; Liu Q; Xu J; Bao B; Shan M; Cao Y; Cheng F; Ding A; Zhang L [Ad] Endereço: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and St [Ti] Título: Application of UHPLC-ESI-Q-TOF-MS to Identify Multiple Constituents in Processed Products of the Herbal Medicine Ligustri Lucidi Fructus. [So] Source: Molecules;22(5), 2017 Apr 26. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Ligustri Lucidi Fructus (LLF), the fruit of Ait. (Oleaceae), has been used as a common herbal medicine in clinical practice in China for nearly 2000 years. In most cases, LLF is prescribed in decoctions in the form of processed products rather than crude drugs. In this study, an ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-ESI-Q-TOF-MS) method was established for rapid separation and identification of multiple constituents in the 80% methanol extract of processed-LLF. A total of 50 compounds (one phenylethanoid, seven phenylethanoid glycosides, seven flavonoids, 25 iridoids, nine triterpenoids and one cyclohexanecarboxylic acid) were either unambiguously identified or tentatively characterized with the aid of authentic standards or published data. Luteolin-7- -rutinoside, oleoside and secologanoside were detected in LLF for the first time. This study enriches the chemical profiling of processed-LLF and could provide valuable information for the quality control and further investigation of processed-LLF and crude LLF. [Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/isolamento & purificação Flavonoides/isolamento & purificação Frutas/química Iridoides/isolamento & purificação Ligustrum/química [Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão Espectrometria de Massas por Ionização por Electrospray Espectrometria de Massas em Tandem Triterpenos/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Iridoids); 0 (Triterpenes) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170808 [Lr] Data última revisão: 170808 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170427 [St] Status: MEDLINE

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[PMID]: 28254481 [Au] Autor: Yang Y; Nian H; Tang X; Wang X; Liu R [Ad] Endereço: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, No.10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, China. [Ti] Título: Effects of the combined Herba Epimedii and Fructus Ligustri Lucidi on bone turnover and TGF-ß1/Smads pathway in GIOP rats. [So] Source: J Ethnopharmacol;201:91-99, 2017 Apr 06. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Kidney deficiency is the main pathogenesis of osteoporosis based on the theory of "kidney governing bones" in traditional Chinese medicine (TCM). Combined Herba Epimedii and Fructus Ligustri Lucidi, based on traditional Chinese formula Er-Zhi pills, were frequently used in TCM formulas that were prescribed for kidney tonifying and bone strengthening. However, it is unclear whether the combination of the two herbs may have a protective influence on glucocorticoid-induced osteoporosis (GIOP). The objective of this study was to evaluate the therapeutic effects and the underlying molecular mechanism of the decoction and the active fractions of the combined herbs in GIOP rats. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into seven groups, including the normal control (NC), GIOP model (MO), active fractions low (100mg/kg, LAF), active fractions high (200mg/kg, HAF), decoction low (3.5g/kg, LD), decoction high (7g/kg, HD) and Calcium with Vitamin D3 (0.2773g/kg, CaD)-treated group. The GIOP model was established by intramuscular injection of dexamethasone (1mg/kg) twice a week for 8 weeks. Different kinds of indicators were measured, including bone mineral density (BMD), bone biomechanical properties, serum bone alkaline phosphatase (b-ALP), serum bone γ-carboxyglutamic acid-containing protein (BGP), serum bone morphogenetic protein-2 (BMP-2), serum tartrate-resistant acid phosphatase (TRACP) and serum carboxy terminal cross linked telopeptide of typeâ… collagen (ICTP), bone mineral content (BMC) and bone structured histomorphometry. The protein and mRNA expression of TGF-ß1, Smad2, Smad3, Smad4 and Smad7 were detected by Western blotting (WB) and quantitative real time polymerase chain reaction (qRT-PCR), respectively. RESULTS: Administration of combined Herba Epimedii and Fructus Ligustri Lucidi decoction and combined active fractions could significantly prevent GC-induced bone loss by increasing the contents of serum b-ALP, BGP and BMP-2 as the markers of bone formation, reducing the serum TRACP and ICTP contents to inhibit bone resorption and enhancing BMC. They could also attenuate biomechanical properties and BMD reduction, deterioration of trabecular architecture in MO rats. The mRNA and protein expressions of TGF-ß1, smad2, smad3 and smad4 were up-regulated, and the mRNA and protein expression of Smad7 was down-regulated following combined Herba Epimedii and Fructus Ligustri Lucidi treatment. CONCLUSION: Combination of Herba Epimedii and Fructus Ligustri Lucidi exhibited protective effects on promoting bone formation and precluding bone resorption. The underlying mechanism may be attributed to its regulations on TGF-ß1/Smads pathway. The substance bases of the combined herbs on anti-osteoporosis were total flavonoids of Herba Epimedii, total iridoids and flavonoids of Fructus Ligustri Lucidi. [Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/farmacologia Medicamentos de Ervas Chinesas/uso terapêutico Epimedium Ligustrum Osteoporose/tratamento farmacológico [Mh] Termos MeSH secundário: Animais Densidade Óssea/efeitos dos fármacos Remodelação Óssea/efeitos dos fármacos Osso e Ossos/efeitos dos fármacos Osso e Ossos/metabolismo Glucocorticoides Masculino Osteoporose/induzido quimicamente Osteoporose/metabolismo Fitoterapia RNA Mensageiro/metabolismo Ratos Sprague-Dawley Proteínas Smad/genética Proteínas Smad/metabolismo Fator de Crescimento Transformador beta1/genética Fator de Crescimento Transformador beta1/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Drugs, Chinese Herbal); 0 (Glucocorticoids); 0 (RNA, Messenger); 0 (Smad Proteins); 0 (Transforming Growth Factor beta1) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170606 [Lr] Data última revisão: 170606 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170304 [St] Status: MEDLINE

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[PMID]: 27916511 [Au] Autor: Seo HL; Baek SY; Lee EH; Lee JH; Lee SG; Kim KY; Jang MH; Park MH; Kim JH; Kim KJ; Lee HS; Ahn SC; Lee JR; Park SJ; Kim SC; Kim YW [Ad] Endereço: Medical Research Center (MRC-GHF), College of Oriental Medicine, Daegu Haany University, Gyeongsan, South Korea. [Ti] Título: Liqustri lucidi Fructus inhibits hepatic injury and functions as an antioxidant by activation of AMP-activated protein kinase in vivo and in vitro. [So] Source: Chem Biol Interact;262:57-68, 2017 Jan 25. [Is] ISSN: 1872-7786 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: Medicinal herbs are used to treat or prevent various diseases, and function to regulate protective mechanisms as nutraceuticals. Fructus Ligustri lucidi is the fruit of Ligustrum lucidum and has been used for its tonic effects on the liver. This study was designed to examine the effects of Fructus Ligustri lucidi water extract (FLL) against severe oxidative stress and mitochondrial impairment in vivo and in vitro and to elucidate its cellular mechanisms of action. Treatment of HepG2 cells with arachidonic acid (AA) + iron successfully induced oxidative stress and apoptosis, as indicated by depletion of glutathione, formation of ROS, decreses in mitochondrial membrane potential (Δψm), and altered expression of apoptosis-related proteins, such as procaspase-3 and Bcl-xL. FLL treatment significantly blocked these pathological changes and the mitochondrial dysfunction caused by AA + iron, which were similar with the effect of aminoimidazole-carboxamide-ß-d-ribofuranoside (AICAR). Moreover, FLL induced the activation of AMP-activated protein kinase (AMPK), which was mediated by its upstream kinase LKB1. Inhibition or activation of AMPK revealed the role of AMPK in cellular protection conferred by FLL in LKB1-deficient cells. In mice, oral administration of 100 mg/kg FLL activated AMPK in the liver, and protected against oxidative stress and liver injury induced by CCl injection. Among the components of FLL, chlorogenic acid was found to be responsible for the protection of hepatocytes against AA + iron-induced cellular damage. Overall, our results confirmed that FLL has the ability to protect hepatocytes against oxidative injury through regulation of the AMPK signaling pathway. [Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo Antioxidantes/farmacologia Ligustrum/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Antioxidantes/química Caspase 3/metabolismo Linhagem Celular Ativação Enzimática/efeitos dos fármacos Frutas/química Frutas/metabolismo Células Hep G2 Seres Humanos Ligustrum/metabolismo Fígado/efeitos dos fármacos Fígado/metabolismo Fígado/patologia Masculino Potencial da Membrana Mitocondrial/efeitos dos fármacos Camundongos Camundongos Endogâmicos C57BL Mitocôndrias/efeitos dos fármacos Mitocôndrias/metabolismo Estresse Oxidativo/efeitos dos fármacos Extratos Vegetais/química Plantas Medicinais/química Plantas Medicinais/metabolismo Proteínas Serina-Treonina Quinases/metabolismo Espécies Reativas de Oxigênio/metabolismo Proteína bcl-X/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 0 (bcl-X Protein); EC 2.7.1.- (STK11 protein, human); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 3.4.22.- (Caspase 3) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161206 [St] Status: MEDLINE

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[PMID]: 28009852 [Au] Autor: Wu J; Ke X; Fu W; Gao X; Zhang H; Wang W; Ma N; Zhao M; Hao X; Zhang Z [Ad] Endereço: Laboratory of Chinese Materia Medica, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, Sichuan, China. jianmingwu@swmu.edu.cn. [Ti] Título: Inhibition of Hypoxia-Induced Retinal Angiogenesis by Specnuezhenide, an Effective Constituent of Ligustrum lucidum Ait., through Suppression of the HIF-1α/VEGF Signaling Pathway. [So] Source: Molecules;21(12), 2016 Dec 21. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Specnuezhenide (SPN), one of the main ingredients of Chinese medicine "Nü-zhen-zi", has anti-angiogenic and vision improvement effects. However, studies of its effect on retinal neovascularization are limited so far. In the present study, we established a vascular endothelial growth factor A (VEGFA) secretion model of human acute retinal pigment epithelial-19 (ARPE-19) cells by exposure of 150 µM CoCl2 to the cells and determined the VEGFA concentrations, the mRNA expressions of VEGFA, hypoxia inducible factor-1α (HIF-1α) & prolyl hydroxylases 2 (PHD-2), and the protein expressions of HIF-1α and PHD-2 after treatment of 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1, 1.0 µg/mL) or SPN (0.2, 1.0 and 5.0 µg/mL). Furthermore, rat pups with retinopathy were treated with SPN (5.0 and 10.0 mg/kg) in an 80% oxygen atmosphere and the retinal avascular areas were assessed through visualization using infusion of ADPase and H&E stains. The results showed that SPN inhibited VEGFA secretion by ARPE-19 cells under hypoxia condition, down-regulated the mRNA expressions of VEGFA and PHD-2 slightly, and the protein expressions of VEGFA, HIF-1α and PHD-2 significantly in vitro. SPN also prevented hypoxia-induced retinal neovascularization in a rat model of oxygen-induced retinopathy in vivo. These results indicate that SPN ameliorates retinal neovascularization through inhibition of HIF-1α/VEGF signaling pathway. Therefore, SPN has the potential to be developed as an agent for the prevention and treatment of diabetic retinopathy. [Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia Glucosídeos/farmacologia Subunidade alfa do Fator 1 Induzível por Hipóxia/antagonistas & inibidores Hipóxia/tratamento farmacológico Ligustrum/química Piranos/farmacologia Neovascularização Retiniana/tratamento farmacológico Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores [Mh] Termos MeSH secundário: Inibidores da Angiogênese/isolamento & purificação Animais Hipóxia Celular Linhagem Celular Cobalto/farmacologia Retinopatia Diabética/tratamento farmacológico Retinopatia Diabética/genética Retinopatia Diabética/metabolismo Retinopatia Diabética/patologia Modelos Animais de Doenças Células Epiteliais/citologia Células Epiteliais/efeitos dos fármacos Células Epiteliais/metabolismo Regulação da Expressão Gênica Glucosídeos/isolamento & purificação Seres Humanos Hipóxia/complicações Hipóxia/genética Hipóxia/patologia Subunidade alfa do Fator 1 Induzível por Hipóxia/genética Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo Prolina Dioxigenases do Fator Induzível por Hipóxia/genética Prolina Dioxigenases do Fator Induzível por Hipóxia/metabolismo Indazóis/farmacologia Extratos Vegetais/química Piranos/isolamento & purificação Ratos Ratos Sprague-Dawley Neovascularização Retiniana/etiologia Neovascularização Retiniana/genética Neovascularização Retiniana/patologia Epitélio Pigmentado da Retina/citologia Epitélio Pigmentado da Retina/efeitos dos fármacos Epitélio Pigmentado da Retina/metabolismo Transdução de Sinais Fator A de Crescimento do Endotélio Vascular/genética Fator A de Crescimento do Endotélio Vascular/secreção [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Angiogenesis Inhibitors); 0 (Glucosides); 0 (HIF1A protein, human); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Indazoles); 0 (Plant Extracts); 0 (Pyrans); 0 (VEGFA protein, human); 0 (Vascular Endothelial Growth Factor A); 154453-18-6 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole); 39011-92-2 (nuezhenide); 3G0H8C9362 (Cobalt); EC 1.14.11.2 (EGLN1 protein, human); EC 1.14.11.29 (Hypoxia-Inducible Factor-Proline Dioxygenases); EVS87XF13W (cobaltous chloride) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170406 [Lr] Data última revisão: 170406 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161224 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 27748884 [Au] Autor: Xu D; Lyu Y; Chen X; Zhu X; Feng J; Xu Y [Ad] Endereço: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, P.R. China. [Ti] Título: Fructus Ligustri Lucidi ethanol extract inhibits osteoclastogenesis in RAW264.7 cells via the RANKL signaling pathway. [So] Source: Mol Med Rep;14(5):4767-4774, 2016 Nov. [Is] ISSN: 1791-3004 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: Fructus ligustri Lucidi (FLL) is the fruit of Ligustrum lucidum Ait and a traditional Chinese medicine, primarily known for its role in osteoporosis prevention and treatment. The present study aimed to elucidate the effect and underlying mechanism of action of ethanol extract of FLL on osteoclast differentiation and bone resorption, and to identify the active compounds within it. RAW264.7 murine monocyte/macrophage cells were stimulated with the receptor activator of nuclear factor κB ligand (RANKL) to induce osteoclast differentiation in vitro. The present study demosntrated that FLL extract and its two primary components, oleanolic acid (OA) and ursolic acid (UA), significantly suppressed RANKL­induced tartrate resistant acid phosphatase (TRAP) activity and multinucleate osteoclast formation without inducing cytotoxicity; however, no effect was observed on the apoptosis of mature osteoclasts. Additionally, RANKL­induced mRNA expression levels of the key transcription factors, tumor necrosis factor receptor associated factor­6, nuclear factor of activated T cell­c1 and c­Fos, and the osteoclast markers, TRAP, cathepsin K and matrix metalloproteinase­9 were suppressed by FLL, OA and UA. However, no effect was observed on RANKL­induced mRNA expression levels of Src. These results demonstrated that FLL may inhibit osteoclastogenesis in RAW264.7 cells via RANKL signaling pathways. OA and UA are active compounds in inducing this effect; however, their specific roles remain to be elucidated. [Mh] Termos MeSH primário: Reabsorção Óssea/metabolismo Frutas/química Ligustrum/química Osteoclastos/efeitos dos fármacos Osteoclastos/metabolismo Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Apoptose/efeitos dos fármacos Reabsorção Óssea/tratamento farmacológico Reabsorção Óssea/genética Linhagem Celular Regulação da Expressão Gênica/efeitos dos fármacos Macrófagos/efeitos dos fármacos Macrófagos/metabolismo Camundongos Ligante RANK/metabolismo Transdução de Sinais/efeitos dos fármacos Fosfatase Ácida Resistente a Tartarato/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Extracts); 0 (RANK Ligand); EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170406 [Lr] Data última revisão: 170406 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161026 [St] Status: MEDLINE [do] DOI: 10.3892/mmr.2016.5849

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[PMID]: 27627920 [Au] Autor: Dong XL; Cao SS; Zhou LP; Denney L; Wong MS; Feng HT [Ad] Endereço: * Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China. [Ti] Título: Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH) D Levels, but Did Not Improve Calcium Balance in Mature Ovariectomized Rats. [So] Source: Am J Chin Med;44(6):1237-1253, 2016. [Is] ISSN: 0192-415X [Cp] País de publicação: Singapore [La] Idioma: eng [Ab] Resumo: Our previous studies found that different extracts or fractions of Fructus ligustri lucidi (FLL) played different roles in altering the regulation of bone and mineral metabolism in different animal models. The present study was designed to compare the actions of FLL ethanol (EE) and water extracts (WE) on bone and mineral metabolism in a 6-month-old mature ovariectomized (OVX) rat model. Our results showed that FLL extracts did not significantly improve systematic Ca balance in mature OVX rats. However, EE, but not WE treatment, significantly increased serum 1,25(OH) D levels in mature OVX rats. An in vitro study using human proximal tubule (HKC-8) cells showed that EE, but not WE, significantly enhanced renal 25-dihydroxyvitamin D -1[Formula: see text]-hydroxylase (1-OHase) mRNA expressions and simultaneously repressed renal 25-dihydroxyvitamin D -24-hydroxylase (24-OHase) mRNA expressions. Further investigation indicated that EE could significantly induce the protein expression of 1-OHase, but did not alter 24-OHase expression in HKC-8 cells. Our results demonstrated that EE increased circulating 1,25(OH) D levels in OVX rats, possibly via upregulation of renal 1-OHase expressions in renal proximal tubule cells. Our study indicates that FLL is a natural oral agent that could directly regulate renal vitamin D metabolism in vivo and in vitro. [Mh] Termos MeSH primário: Calcitriol/metabolismo Cálcio/metabolismo Etanol Ligustrum/química Ovariectomia Extratos Vegetais/isolamento & purificação Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: 25-Hidroxivitamina D3 1-alfa-Hidroxilase/genética 25-Hidroxivitamina D3 1-alfa-Hidroxilase/metabolismo Animais Osso e Ossos/metabolismo Feminino Frutas/química Expressão Gênica/efeitos dos fármacos Seres Humanos Túbulos Renais Proximais/citologia Túbulos Renais Proximais/metabolismo Modelos Animais Ratos Sprague-Dawley Regulação para Cima/efeitos dos fármacos Vitamina D3 24-Hidroxilase/genética Vitamina D3 24-Hidroxilase/metabolismo Água [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Extracts); 059QF0KO0R (Water); 3K9958V90M (Ethanol); EC 1.14.13.13 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase); EC 1.14.15.16 (Cyp24a1 protein, rat); EC 1.14.15.16 (Vitamin D3 24-Hydroxylase); FXC9231JVH (Calcitriol); SY7Q814VUP (Calcium) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170119 [Lr] Data última revisão: 170119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160916 [St] Status: MEDLINE

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