Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.583.670.175 [Categoria DeCS]
Referências encontradas : 175 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 18 ir para página                         

  1 / 175 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29360858
[Au] Autor:Zhang A; Yang X; Li Q; Yang Y; Zhao G; Wang B; Wu D
[Ad] Endereço:Xinjiang Key Lab of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China.
[Ti] Título:Immunostimulatory activity of water-extractable polysaccharides from Cistanche deserticola as a plant adjuvant in vitro and in vivo.
[So] Source:PLoS One;13(1):e0191356, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A safe and effective vaccine adjuvant is important in modern vaccines. Various Chinese herbal polysaccharides can activate the immune system. Cistanche deserticola (CD) is a traditional Chinese herb and an adjuvant candidate. Here, we confirmed that water-extractable polysaccharides of CD (WPCD) could modulate immune responses in vitro and in vivo. In a dose-dependent manner, WPCD significantly promoted the maturation and function of murine marrow-derived dendritic cells (BM-DCs) through up-regulating the expression levels of MHC-II, CD86, CD80, and CD40, allogenic T cell proliferation, and the yields of IL-12 and TNF-α via toll-like receptor4 (TLR4), as indicated by in vitro experiments. In addition, its immunomodulatory activity was also observed in mice. WPCD effectively improved the titers of IgG, IgG1 and IgG2a and markedly enhanced the proliferation of T and B cells, the production of IFN-γ and IL-4 in CD4+ T cells and the expression level of IFN-γ in CD8+ T cells better than Alum. Furthermore, WPCD could markedly up-regulate the expression levels of CD40 and CD80 on DCs in spleen and down-regulate the Treg frequency. The study suggests that polysaccharides of Cistanche deserticola are a safe and effective vaccine adjuvant for eliciting both humoral immunity and cellular immunity by activating DCs via TLR4 signaling pathway.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Cistanche/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Adjuvantes Imunológicos/isolamento & purificação
Animais
Diferenciação Celular/efeitos dos fármacos
Células Dendríticas/citologia
Células Dendríticas/efeitos dos fármacos
Células Dendríticas/imunologia
Medicamentos de Ervas Chinesas/administração & dosagem
Medicamentos de Ervas Chinesas/farmacologia
Feminino
Imunidade Celular/efeitos dos fármacos
Imunidade Humoral/efeitos dos fármacos
Imunogenicidade da Vacina/efeitos dos fármacos
Técnicas In Vitro
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos ICR
Ovalbumina/administração & dosagem
Ovalbumina/imunologia
Plantas Medicinais/química
Polissacarídeos/administração & dosagem
Polissacarídeos/isolamento & purificação
Solubilidade
Linfócitos T Reguladores/efeitos dos fármacos
Linfócitos T Reguladores/imunologia
Receptor 4 Toll-Like/metabolismo
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Drugs, Chinese Herbal); 0 (Polysaccharides); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 059QF0KO0R (Water); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191356


  2 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28771352
[Au] Autor:Li Y; Peng Y; Wang M; Tu P; Li X
[Ad] Endereço:School of Pharmacy, Shanghai Jiao Tong University , Shanghai 200240, China.
[Ti] Título:Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.
[So] Source:J Agric Food Chem;65(34):7447-7456, 2017 Aug 30.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cistanches Herba is taken orally as a health food supplement and medicinal plant in Asian countries. It consists of the stems of Cistanche deserticola (CD) and Cistanche tubulosa (CT). The gastrointestinal metabolism of the multiple components contained in Cistanches Herba is crucial for the discovery of bioactive constituents. This study aims to elucidate the comprehensive metabolic profile of the Cistanches Herba water extract by simulating human gastrointestinal metabolism in vitro independently and sequentially using four models: gastric juice, intestinal juice, human intestinal bacteria, and human intestinal microsomes. A total of 35 and 18 metabolites were characterized from CD and CT water extracts, respectively. These metabolites were formed through reduction, methylation, dimethylation, deglycosylation, decaffeoyl, derhamnose, dehydrogenation, and glucuronidation. The difference in metabolites of the Cistanches Herba water extract and single compounds and the difference in metabolites of CD and CT water extracts were caused by the oligosaccharides and polysaccharides in Cistanches Herba.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Cistanche/química
Medicamentos de Ervas Chinesas/metabolismo
Suco Gástrico/metabolismo
Microbioma Gastrointestinal
Intestinos/microbiologia
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/genética
Bactérias/isolamento & purificação
Seres Humanos
Intestinos/metabolismo
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02829


  3 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28476319
[Au] Autor:Yan Y; Song Q; Chen X; Li J; Li P; Wang Y; Liu T; Song Y; Tu P
[Ad] Endereço:Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China; Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
[Ti] Título:Simultaneous determination of components with wide polarity and content ranges in Cistanche tubulosa using serially coupled reverse phase-hydrophilic interaction chromatography-tandem mass spectrometry.
[So] Source:J Chromatogr A;1501:39-50, 2017 Jun 09.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To meet the demands from plant physiologists and pharmacognosists, sustainable efforts are being devoted by the analytical chemists from all over the world to search an approach being capable of simultaneously monitoring primary along with secondary metabolites. The key technical bottlenecks currently lie at affording satisfactory chromatographic and spectrometric performances for both hydrophilic and hydrophobic substances that span a great content range. Herein, reverse phase liquid chromatography was directly coupled with hydrophilic interaction chromatography, namely RPLC-HILIC, to integrate their merits, whereas dilution pumps were employed to tackle the mismatching for the mobile phase between them. On the other side, inferior parameters rather than the optimal ones were applied for those abundant ingredients to advance the upper limits of quantitation, such as echinacoside (1250.0µg/mL), mannitol (100.0µg/mL), and acteoside (125µg/mL), in mass spectrometer domain. As a desert parasitic plant as well as tonic materials, Cistanche tubulosa (CT) has drawn widely interests from plant physiologists and pharmacognosists regarding its quantitative metabolome. Simultaneous determination of 23 abundant and minor ingredients covering most chemical families in CT, i.e. amino acid, nucleoside, organic acid, phenylethanoid glycoside, lignan, and iridoid, was attempted to understand the physiologic patterns as well as pharmacological values of the crude materials. Although the analytes spanned wide polarity (-3.326≤cLogP≤1.421) and content (more than 5 orders of magnitude) ranges, satisfactory chromatographic and spectrometric behaviors were gained for all analytes. Reliable quantitation was demonstrated via various method validation assays, such as recovery, linearity, sensitivity, and precision. The contents of 23 hydrophilic and hydrophobic substances were quantified in 20 batches of CT. Significant variations occurred for the contents of those components. Echinacoside (1.35-387.50mg/g) was usually observed as the most abundant component, whereas ferulic acid (<0.0043mg/g) always exhibited trace distributions. Above all, the integrated equipment setup could serve as a fit-for-purpose tool for in-depth quality evaluation of CT and more importantly, for comprehensively understanding the metabolome of plants.
[Mh] Termos MeSH primário: Cromatografia de Fase Reversa/métodos
Cistanche/química
Extratos Vegetais/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida/métodos
Glucosídeos/análise
Glicosídeos/análise
Interações Hidrofóbicas e Hidrofílicas
Metaboloma
Nucleosídeos
Fenóis/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosides); 0 (Glycosides); 0 (Nucleosides); 0 (Phenols); 0 (Plant Extracts); 3TGX09BD5B (acteoside); I04O1DT48T (echinacoside)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE


  4 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28257663
[Au] Autor:Lin WY; Yao C; Cheng J; Kao ST; Tsai FJ; Liu HP
[Ad] Endereço:Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
[Ti] Título:Molecular pathways related to the longevity promotion and cognitive improvement of Cistanche tubulosa in Drosophila.
[So] Source:Phytomedicine;26:37-44, 2017 Mar 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The aging process, including physical dysfunction and age-related memory impairment (AMI), are considered to be correlated with cumulative oxidative damages and insulin/IGF-1 signaling pathway. PURPOSE: The present study was to elucidate the in vivo effects on delaying aging and ameliorating AMI and underlying molecular mechanisms of Cistanche tubulosa (CT), a herb used in traditional Chinese medicine to improve sexual function and treat kidney dysfunction. METHODS: The flies, treated and untreated with CT, were observed for lifespan, resistance to oxidative stress with H O or paraquat, starvation assay, cognitive behaviors with T-maze, and transcript levels of target genes with quantitative RT-PCR. RESULTS: Administering CT extended the mean and maximum lifespan and increased resistance to oxidative stress in flies. CT supplementation also enhanced memory formation in young flies and suppressed AMI upon aging. Several genes and signaling pathways, including the mechanistic target of rapamycin (mTOR) and Notch networks, have been identified as causing these pharmacological effects and alterations in the gene expression of glutamate receptors. CONCLUSION: Our results indicate that CT supplementation may contribute to slowing aging phenotypes and alleviating cognitive behavioral decline in flies, indicating potential applicability for enhancing human health and reducing susceptibility to age-related disorders.
[Mh] Termos MeSH primário: Envelhecimento/efeitos dos fármacos
Cistanche/química
Cognição/efeitos dos fármacos
Drosophila/efeitos dos fármacos
Medicamentos de Ervas Chinesas/farmacologia
Longevidade/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  5 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28138704
[Au] Autor:Yang J; Xu H; Wu S; Ju B; Zhu D; Yan Y; Wang M; Hu J
[Ad] Endereço:Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
[Ti] Título:Preparation and evaluation of microemulsion­based transdermal delivery of Cistanche tubulosa phenylethanoid glycosides.
[So] Source:Mol Med Rep;15(3):1109-1116, 2017 Mar.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The primary aim of the present study was to develop a novel microemulsion (ME) formulation to deliver phenylethanoid glycoside (PG) for use in skin lighteners and sunscreens. The oil phase was selected on the basis of drug solubility, while the surfactant and cosurfactant were screened and selected on the basis of their solubilizing capacity and the efficiency with which they formed MEs. Pseudoternary phase diagrams were constructed to evaluate ME regions and five formulations of oil­in­water MEs were selected as vehicles. In vitro skin permeation experiments were performed to optimize the ME formulation and to evaluate its permeability in comparison to that of saline solution. The physicochemical properties of the optimized ME and the permeating ability of PG delivered by this ME were also investigated. The optimized ME formulation was composed of isopropyl myristate (7%, w/w), Cremorphor EL (21%, w/w), propylene glycol (7%, w/w) and water (65%, w/w). The cumulative amount of PG that permeated through excised mouse skin when carried by ME was ~1.68 times that when PG was carried by saline solution only. The cumulative amount of PG in the microemulsion (4149.650±37.3 µg·cm­2) was significantly greater than that of PG in the saline solution (2288.63±20.9 µg·cm­2). Furthermore, the permeability coefficient indicated that optimized microemulsion was a more efficient carrier for transdermal delivery of PG than the control solution (8.87±0.49 cm/hx10­3 vs. 5.41±0.12 cm/hx10­3). Taken together, the permeating ability of ME­carried PG was significantly increased compared with saline solution.
[Mh] Termos MeSH primário: Cistanche/química
Emulsões
Glicosídeos/administração & dosagem
Glicosídeos/química
Álcool Feniletílico/química
[Mh] Termos MeSH secundário: Administração Cutânea
Animais
Sistemas de Liberação de Medicamentos
Estabilidade de Medicamentos
Masculino
Camundongos
Permeabilidade
Preparações de Plantas/administração & dosagem
Preparações de Plantas/química
Pele/efeitos dos fármacos
Pele/metabolismo
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Glycosides); 0 (Plant Preparations); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6147


  6 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27904049
[Au] Autor:Peng F; Xu R; Wang X; Xu C; Liu T; Chen J
[Ad] Endereço:Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College.
[Ti] Título:Effect of the Steaming Process on Quality of Postharvest Cistanche deserticola for Medicinal Use during Sun Drying.
[So] Source:Biol Pharm Bull;39(12):2066-2070, 2016.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Effect of steaming on postharvest stems of Cistanche deserticola Y. C. MA was evaluated periodically during sun drying in both years. Steamed stems exhibited oily black in color, slightly heavier and longer than untreated ones. The levels of five phenylethanoid glycosides and 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging activity in steamed stems remained relatively stable during sun drying. Steamed samples showed higher amounts of water-soluble extracts, dilute ethanol-soluble extracts, soluble sugars, and polysaccharides, while a lower level of total ashes than untreated samples. The overall results suggest that steaming is an effective processing for enhancing the appearance quality and the concentration of some bioactive compounds in Cistanches Herba.
[Mh] Termos MeSH primário: Cistanche
Extratos Vegetais/química
Caules de Planta/química
Vapor
[Mh] Termos MeSH secundário: Compostos de Bifenilo/química
Dessecação
Depuradores de Radicais Livres/química
Glicosídeos/análise
Picratos/química
Plantas Medicinais
Polissacarídeos/análise
Luz Solar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Glycosides); 0 (Picrates); 0 (Plant Extracts); 0 (Polysaccharides); 0 (Steam); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE


  7 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27639339
[Au] Autor:Li Y; Peng Y; Wang M; Zhou G; Zhang Y; Li X
[Ad] Endereço:School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
[Ti] Título:Rapid screening and identification of the differences between metabolites of Cistanche deserticola and C. tubulosa water extract in rats by UPLC-Q-TOF-MS combined pattern recognition analysis.
[So] Source:J Pharm Biomed Anal;131:364-372, 2016 Nov 30.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cistanches Herba is a famous traditional Chinese medicine that has been in use for treating kidney deficiency, impotence, female infertility, morbid leucorrhea, profuse metrorrhagia, and senile constipation. With the exception of studies available for a few single active ingredients such as echinacoside, acteoside, and poliumoside, comprehensive and systematic studies on in vivo metabolism of Cistanches Herba are lacking despite its widespread clinical application. There is no comparative study yet on the metabolites resulting from the traditional usage of Cistanche deserticola and C. tubulosa water extract - two species that are recorded in Chinese Pharmacopoeia. This further restricts research on the therapeutic effect of Cistanches Herba to a great extent. In this study, a robust and unbiased UPLC-Q-TOF-MS combined pattern recognition analysis (orthogonal partial least squared discriminant analysis, OPLS-DA) was employed to rapidly screen prototype components and metabolites of C. deserticola and C. tubulosa water extract in rat urine, feces, and serum. A total of 71 metabolites from C. deserticola including 25 prototype components and 46 metabolites, and 45 metabolites from C. tubulosa including 18 prototype components and 27 metabolites were tentatively identified. Out of these, 10 metabolites were characterized for the first time in these two species. Results of this study indicate that phenylethanoid glycosides (PhGs) are mainly metabolized into degradation products in the gastrointestinal tract of rats. The chemical components cistanoside B, C, D, and E exist only in C. deserticola and release methylated hydroxytyrosol (HT) following metabolism. This factor contributes to the difference between metabolites of C. deserticola and C. tubulosa water extract in rats and is responsible for the differential therapeutic effect that these two species of Cistanches Herba have on the same diseases.
[Mh] Termos MeSH primário: Cistanche
Medicamentos de Ervas Chinesas/análise
Medicamentos de Ervas Chinesas/metabolismo
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Masculino
Caules de Planta
Distribuição Aleatória
Ratos
Ratos Sprague-Dawley
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 059QF0KO0R (Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171008
[Lr] Data última revisão:
171008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160919
[St] Status:MEDLINE


  8 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27637449
[Au] Autor:Guo Y; Cao L; Zhao Q; Zhang L; Chen J; Liu B; Zhao B
[Ad] Endereço:Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing,100049, PR China.
[Ti] Título:Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola.
[So] Source:Int J Biol Macromol;93(Pt A):678-685, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To research the preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharides from Cistanche deserticola (CDPs), three polysaccharide fractions, CDP-A, CDP-B and CDP-C, were obtained by successively membrane filtration (microfiltration, ultrafiltration and nanofiltration). Molecular weights, monosaccharide compositions, purities and IR spectra of the three fractions were analyzed. Results showed that CDP-C contained higher proportion of galacturonic acid (GalUA) than CDP-B and CDP-A. Antioxidant activities were also analyzed and the results revealed that CDP-C possessed the highest activity. Thus, hepatoprotective activity of CDP-C was studied further. In vitro research, CDP-C promoted viability of HepG2 cells. In vivo research, CDP-C ameliorated the alterations induced by alcohol, including serological indexes (alanine transaminase, acid phosphatase, γ-glutamyl transpeptidase and triglyceride) and hepatic indicators (superoxide dismutase, malondialdehyde, glutathione S-transferase and triglyceride) in model animals. The prominent microvesicular steatosis and mild necrosis in hepatic histopathology of model animals were also attenuated by CDP-C administration. These findings indicated that CDP-C possessed hepatoprotective activity against chronic hepatic injury induced by alcohol. The underlying mechanism might be that CDP-C can reduce the contents of MDA and TG, and modulate the activities of the relative enzyme. This property might associate with GalUA in CDP-C.
[Mh] Termos MeSH primário: Antioxidantes/química
Antioxidantes/farmacologia
Cistanche/química
Citoproteção/efeitos dos fármacos
Fígado/efeitos dos fármacos
Polissacarídeos/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Etanol/toxicidade
Feminino
Células Hep G2
Seres Humanos
Fígado/citologia
Fígado/lesões
Fígado/metabolismo
Camundongos
Camundongos Endogâmicos ICR
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Polysaccharides); 11062-77-4 (Superoxides); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160918
[St] Status:MEDLINE


  9 / 175 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27593241
[Au] Autor:Zhang W; Huang J; Wang W; Li Q; Chen Y; Feng W; Zheng D; Zhao T; Mao G; Yang L; Wu X
[Ad] Endereço:School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
[Ti] Título:Extraction, purification, characterization and antioxidant activities of polysaccharides from Cistanche tubulosa.
[So] Source:Int J Biol Macromol;93(Pt A):448-458, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An efficient ultrasonic-cellulase-assisted extraction (UCE) of Cistanche tubulosa polysaccharide (CTP) was established. The response surface methodology based on Box-Behnken Design was employed to further optimize extraction conditions. After quaternary ammonium salt precipitation, the polysaccharide of C. tubulosa was characterized by different techniques. The results showed that a maximum polysaccharide yield of 22.31±0.45% was achieved at a pH of 5.2 for 31.5min at 54.1°C. Compared to hot water extraction, the yield of CTP in UCE and polysaccharide content increased to 44.96% and 70.13±2.19%, respectively. There was no marked difference among polysaccharides extracted using different methods from the infrared spectrum. Ultrasonic-cellulase-assisted extraction polysaccharide showed a fibrous structure from scanning electron microscopy and was composed of rhamnose, mannose, glucose, and galactose in a molar ratio of 2.18:1:28.29:1.43 by gas chromatography. The circular dichroism results indicated that polysaccharides had a maximum positive peak around 210nm with different peak values. The thermogravimetric analysis and differential scanning calorimetry were used to test the thermostability of CTP. Besides, CTP demonstrated appreciable antioxidant potential on antioxidant experiments in vitro. The results suggested that UCE is an effective method for CTP extraction and its polysaccharide showed appreciable antioxidant activity.
[Mh] Termos MeSH primário: Cistanche/química
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/isolamento & purificação
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radical Scavengers); 0 (Polysaccharides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE


  10 / 175 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27493021
[Au] Autor:Li M; Li Y; Liu W; Li R; Qin C; Liu N; Han J
[Ad] Endereço:School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, China.
[Ti] Título:The preparation of Cistanche phenylethanoid glycosides liquid proliposomes: Optimized formulation, characterization and proliposome dripping pills in vitro and in vivo evaluation.
[So] Source:Eur J Pharm Sci;93:224-32, 2016 Oct 10.
[Is] ISSN:1879-0720
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Water-soluble Cistanche phenylethanoid glycosides (CPhGs) have poor permeability and low bioavailability. However, liposomes can improve the permeability of such drugs and their poor stability, and proliposomes have been used to overcome these problems. Based on this, Cistanche phenylethanoid glycoside liquid proliposomes (CPhGsP) and dripping(?) pills were prepared and optimized using response surface methodology. The properties of CPhGsP were evaluated in terms of their encapsulation efficiency, particle size, zeta potential, and morphology. The results obtained showed that the optimal formulation was drug/soybean phospholipid/poloxamer-188/sodium deoxycholate/propylene glycol 1:22.38:3.52:0.84:80 (w/w/w/w/v). This resulted in an encapsulation efficiency, particle size, and zeta potential of hydrated proliposomes with phosphate buffer solution (pH7.4) of 51.97%, 671.7nm, and -25.49mV, respectively. Stability testing of CPhGsP and CPhGs ordinary liposomes was carried out for 3months at 4±2°C, 25±2°C, 40±2°C, 75±5% RH. The results obtained showed that the stability of the proliposomes was better than that of ordinary liposomes at the same temperature, while a lower temperature of 4°C is ideal for storage. Cistanche phenylethanoid glycoside liquid proliposomes dripping pills (CPhGsPD) are efficiently released in gastrointestinal solution as shown by in vitro release experiments and the structure of the liposomes does not destroy the proliposome dripping pills by hydration. In vivo experiments showed that the areas under the plasma level-time curves and peak concentrations of CPhGsPD and hydrated proliposomes were higher than those of CPhGs. Moreover, with CPhGsPD, the pharmacokinetic parameters were similar to those with hydrated proliposomes. These results showed that CPhGsPD offer a good way to improve the oral delivery of CPhGs.
[Mh] Termos MeSH primário: Cistanche
Glicosídeos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Disponibilidade Biológica
Ácido Desoxicólico/química
Composição de Medicamentos
Glicosídeos/química
Glicosídeos/farmacocinética
Lipossomos
Masculino
Fosfolipídeos/química
Poloxâmero/química
Propilenoglicol/química
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Liposomes); 0 (Phospholipids); 005990WHZZ (Deoxycholic Acid); 106392-12-5 (Poloxamer); 6DC9Q167V3 (Propylene Glycol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE



página 1 de 18 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde