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Pesquisa : B01.650.940.800.575.912.250.583.700.850 [Categoria DeCS]
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[PMID]:28214376
[Au] Autor:Ali A; Haq FU; Ul Arfeen Q; Sharma KR; Adhikari A; Musharraf SG
[Ad] Endereço:H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
[Ti] Título:Sensitive quantification of coixol, a potent insulin secretagogue, in Scoparia dulcis extract using high-performance liquid chromatography combined with tandem mass spectrometry and UV detection.
[So] Source:Biomed Chromatogr;31(10), 2017 Oct.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Diabetes is a major global health problem which requires new studies for its prevention and control. Scoparia dulcis, a herbal product, is widely used for treatment of diabetes. Recent studies demonstrate coixol as a potent and nontoxic insulin secretagog from S. dulcis. This study focuses on developing two quantitative methods of coixol in S. dulcis methanol-based extracts. Quantification of coixol was performed using high-performance liquid chromatography-tandem mass spectrometry (method 1) and high-performance liquid chromatography-ultraviolet detection (method 2) with limits of detection of 0.26 and 11.6 pg/µL, respectively, and limits of quantification of 0.78 and 35.5 pg/µL, respectively. S. dulcis is rich in coixol content with values of 255.5 ± 2.1 mg/kg (method 1) and 220.4 ± 2.9 mg/kg (method 2). Excellent linearity with determination coefficients >0.999 was achieved for calibration curves from 10 to 7500 ng/mL (method 1) and from 175 to 7500 ng/mL (method 2). Good accuracy (bias < -8.6%) and precision (RSD < 8.5%) were obtained for both methods. Thus, they can be employed to analyze coixol in plant extracts and herbal formulations.
[Mh] Termos MeSH primário: Benzoxazóis/análise
Cromatografia Líquida de Alta Pressão/métodos
Extratos Vegetais/química
Scoparia/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Insulina/análogos & derivados
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoxazoles); 0 (Insulin); 0 (Plant Extracts); 532-91-2 (6-methoxybenzoxazolinone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3964


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[PMID]:28012927
[Au] Autor:Wankhar W; Srinivasan S; Sundareswaran L; Wankhar D; Rajan R; Sheeladevi R
[Ad] Endereço:Department of Physiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
[Ti] Título:Role of Scoparia dulcis linn on noise-induced nitric oxide synthase (NOS) expression and neurotransmitter assessment on motor function in Wistar albino rats.
[So] Source:Biomed Pharmacother;86:475-481, 2017 Feb.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Noise pollution is one of the most widespread and fast growing environmental and occupational menaces in the modern era. Exposure to noise above 100dB is not adaptable through the brain homeostatic mechanism. Yet, the detrimental effects of noise have often been ignored. Developing reliable animal models to understand the neurobiology of noise stress and advance our research in the field of medicine to impede this growing stressor is needed. In this study experimental animals were divided into four groups, (i) Control and (ii) S. dulcis extract (200mg/kgbw) treated control group. (iii) To mimic the influence of noise, animals in this group were exposed to noise stress (100dB/4h/day) for 15days and finally, (iv) Noise exposed treated with S. dulcis extract (200mg/kgbw) group. Rota-rod and narrow beam performance results showed impaired motor co-ordination in noise exposed group on both 1st and 15th day when compared to controls. This impaired motor function on exposure to noise could be attributed to the altered norepinephrine, dopamine and serotonin levels in both the striatum and cerebellum. Moreover, the motor impaired associated changes could also be attributed to upregulated nNOS and iNOS protein expression in the cerebellum resulting in increased nitric oxide radical production. This increased reactive free radicals species can initiate lipid peroxidation mediated changes in the cerebellar Purkinje cells, which is responsible for initiating inhibitory motor response and ultimately leading to impaired motor co-ordination. Treatment with S. dulcis extract (200mg/kgbw) could control motor impairment and regulate neurotransmitter level as that of control groups when compared to noise exposed group. One key aspect of therapeutic efficacy of the plant could have resulted due to attenuated lipid peroxidation mediated damages on the cerebellar Purkinje cells thereby regulating motor impairment. Thus, targeting the antioxidant and free radicals scavenging properties of the plant could serve as a potential therapeutic to combat this environmental stressor.
[Mh] Termos MeSH primário: Atividade Motora/efeitos dos fármacos
Neurotransmissores/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Ruído/efeitos adversos
Extratos Vegetais/farmacologia
Scoparia/química
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Masculino
Óxido Nítrico/metabolismo
Células de Purkinje/efeitos dos fármacos
Células de Purkinje/metabolismo
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Neurotransmitter Agents); 0 (Plant Extracts); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


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[PMID]:26597857
[Au] Autor:Abere TA; Okoye CJ; Agoreyo FO; Eze GI; Jesuorobo RI; Egharevba CO; Aimator PO
[Ad] Endereço:Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria. eseabere@yahoo.com.
[Ti] Título:Antisickling and toxicological evaluation of the leaves of Scoparia dulcis Linn (Scrophulariaceae).
[So] Source:BMC Complement Altern Med;15:414, 2015 Nov 23.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Scoparia dulcis Linn (Scrophulariaceae) together with other medicinal plants serve as antisickling remedies in Africa. This study was aimed at investigating the antisickling activity of the leaves of the plant as well as establishing the toxicological profile. METHOD: Chemical tests were employed in phytochemical investigations. Evaluation of the antisickling activity involved the inhibition of sodium metabisulphite-induced sickling of the HbSS red blood cells obtained from confirmed sickle cell patients who were not in crises. Concentrations of the crude extract and its fractions were tested with normal saline and p-hydroxybenzoic acid serving as controls. Acute toxicological evaluation was carried out in mice while 30-day assessment was done in rats. RESULTS: Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids and saponins. Percentage sickling inhibitions of the aqueous methanol extracts of S. dulcis were significant all through the period of assay p < 0. 05 compared to normal saline, but not significant with PHBA. The fractions had less activity compared to the crude extracts. The LD 50 of the extract in mice was above 8000 mg/kg body weight when administered orally. Toxicological evaluations at 250 and 500 mg/kg showed mild congestion in virtually all the target organs. CONCLUSION: The antisickling results confirmed traditional usage of Scoparia dulcis in the management of Sickle cell disorders and a candidate for further investigations.
[Mh] Termos MeSH primário: Anemia Falciforme/tratamento farmacológico
Antidrepanocíticos/administração & dosagem
Avaliação Pré-Clínica de Medicamentos
Extratos Vegetais/administração & dosagem
Scoparia/química
[Mh] Termos MeSH secundário: Animais
Antidrepanocíticos/química
Antidrepanocíticos/toxicidade
Eritrócitos/efeitos dos fármacos
Feminino
Seres Humanos
Masculino
Camundongos
Extratos Vegetais/química
Extratos Vegetais/toxicidade
Ratos
Ratos Wistar
Scoparia/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antisickling Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151126
[Lr] Data última revisão:
151126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-015-0928-5


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[PMID]:26582144
[Au] Autor:Senadheera SP; Ekanayake S; Wanigatunge C
[Ad] Endereço:Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
[Ti] Título:Anti-hyperglycaemic effects of herbal porridge made of Scoparia dulcis leaf extract in diabetics - a randomized crossover clinical trial.
[So] Source:BMC Complement Altern Med;15:410, 2015 Nov 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leaf extracts of Scoparia dulcis, is used as a herbal remedy by diabetics worldwide. Fresh Scoparia dulcis porridge elicited a low glycaemic index (GI) and anti-hyperglycaemic effects when fed to diabetic Wistar rats. Commercially produced Scoparia dulcis porridge (SDC) elicited medium GI. Present study was aimed at studying the anti-diabetic effects of consumption of commercially produced S. dulcis porridge. METHOD: A randomized crossover clinical trial with type 2 diabetic patients (n = 35) on medication, with mild and moderate diabetes [fasting blood glucose (FBG) 126-300 mg/dL, age 35-70 years] was conducted. Within the first three months (study period 1) group 1 was the test and group 2 was the control. Following a wash-out period, the two groups were crossed over (study period 2: group 1 - control; group 2 - test). Test group consumed commercially produced SDC for 3 days/week for three months and the control group any other food. At the onset and end of each study period glucose measurements [Fasting Blood Glucose (FBG), HbA1c], lipid measurements (total cholesterol, HDL-C, LDL-C, triglycerides, cholesterol ratios), toxicity parameters (liver enzymes, creatinine, CRP, eGFR) were analyzed by enzyme assay kit methods using a KONELAB 20XT auto analyzer. Significances between groups were analyzed by one way ANOVA (normal distribution) and Mann Whitney test (if the values were not normally distributed). Within group comparisons were carried out by Bonferroni post hoc test. RESULTS: During the crossover clinical trial HbA1c of group 1 decreased from 7.9 ± 0.5 to 6.5 ± 0.3 (p = 0.003) while HbA1c of group 2 decreased from 7.0 ± 0.3to 6.7 ± 0.3 while in the test group. Therefore, both test groups (1 and 2) elicited a decrease in HbA1c compared to respective control groups. Both test groups elicited a non significant decrease in FBG following the intervention (group 1 - from 174 ± 14 to 160 ± 10 mg/dL; group 2 - from 183 ± 13 to 160 ± 7 mg/dL). No significant differences (p >0.05) in insulin, cholesterol measurements (total cholesterol, LDL-C, HDL-C, triglycerides and cholesterol ratios) and atherogenic index between or within groups were observed. All other measurements (AST, ALT, ALP, creatinine, CRP, eGFR) were normal and not significantly different between or within groups. CONCLUSION: Porridge made with SDC leaf extract decreased FBG and HbA1c (p >0.05) of type 2 diabetic patients. The porridge had no effect on cholesterol measurements and no toxicity was observed at the dose tested. Therefore, the SDC porridge can be recommended as a suitable meal for diabetic patients.
[Mh] Termos MeSH primário: Diabetes Mellitus/tratamento farmacológico
Hiperglicemia/tratamento farmacológico
Hipoglicemiantes/uso terapêutico
Fitoterapia
Scoparia
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Glicemia/efeitos dos fármacos
Estudos Cross-Over
Diabetes Mellitus/dietoterapia
Grãos Comestíveis
Feminino
Seres Humanos
Hiperglicemia/dietoterapia
Hipoglicemiantes/farmacologia
Masculino
Meia-Idade
Oryza
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
Plantas Medicinais
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151120
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-015-0935-6


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[PMID]:26345907
[Au] Autor:de Freitas PL; Dias AC; Moreira VR; Monteiro SG; Pereira SR
[Ad] Endereço:Laboratório de Genética e Biologia Molecular, Departamento de Biologia, Universidade Federal do Maranhão, São Luís, MA, Brasil.
[Ti] Título:Antimutagenic action of the triterpene betulinic acid isolated from Scoparia dulcis (Scrophulariaceae).
[So] Source:Genet Mol Res;14(3):9745-52, 2015 Aug 19.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The mutagenic and antimutagenic activities of triterpene betulinic acid {3b-3-hydroxy-lup-20(29)-en-28-oic} isolated from the roots of Scoparia dulcis (Scrophulariaceae) were analyzed using the somatic mutation and recombination test (SMART) in the wings of Drosophila melanogaster. The mutagenic potential of betulinic acid was evaluated at 3 different concentrations (1.64, 3.28, and 6.57 mM). Antimutagenic activity evaluation was performed by co-treatment trials in which the flies received betulinic acid at 3 different concentrations in addition to 10 mM pro-mutagenic urethane. The results demonstrated that betulinic acid was not capable of causing DNA damage. However, the frequency of small single spots, large spots, and twin spots was significantly reduced. In the high bioactivation cross, betulinic acid was significantly active and exerted enhanced antimutagenic activity, possibly as a desmutagen.
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Extratos Vegetais/farmacologia
Scoparia/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antimutagênicos/química
Drosophila melanogaster/efeitos dos fármacos
Drosophila melanogaster/genética
Feminino
Masculino
Testes de Mutagenicidade/métodos
Extratos Vegetais/química
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimutagenic Agents); 0 (Plant Extracts); 0 (Triterpenes); 4G6A18707N (betulinic acid)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150908
[Lr] Data última revisão:
150908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE
[do] DOI:0.4238/2015.August.19.7


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[PMID]:26178652
[Au] Autor:Sharma KR; Adhikari A; Hafizur RM; Hameed A; Raza SA; Kalauni SK; Miyazaki J; Choudhary MI
[Ad] Endereço:H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
[Ti] Título:Potent Insulin Secretagogue from Scoparia dulcis Linn of Nepalese Origin.
[So] Source:Phytother Res;29(10):1672-5, 2015 Oct.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ethno-botanical inspired isolation from plant Scoparia dulcis Linn. (Sweet Broomweed) yielded six compounds, coixol (1), glutinol (2), glutinone (3), friedelin (4), betulinic acid (5), and tetratriacontan-1-ol (6). There structures were identified using mass and 1D- and 2D-NMR spectroscopy techniques. Compounds 1-6 were evaluated for their insulin secretory activity on isolated mice islets and MIN-6 pancreatic ß-cell line, and compounds 1 and 2 were found to be potent and mildly active, respectively. Compound 1 was further evaluated for insulin secretory activity on MIN-6 cells. Compound 1 was subjected to in vitro cytotoxicity assay against MIN-6, 3T3 cell lines, and islet cells, and in vivo acute toxicity test in mice that was found to be non-toxic. The insulin secretory activity of compounds 1 and 2 supported the ethno-botanic uses of S. dulcis as an anti-diabetic agent.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/tratamento farmacológico
Hipoglicemiantes/farmacologia
Extratos Vegetais/uso terapêutico
Scoparia
[Mh] Termos MeSH secundário: Células 3T3
Animais
Insulina
Ilhotas Pancreáticas
Masculino
Camundongos
Nepal
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Insulin); 0 (Plant Extracts)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:151009
[Lr] Data última revisão:
151009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150717
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5412


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[PMID]:25885934
[Au] Autor:Saidi SA; Bourogâa E; Bouaziz A; Mongi S; Chaaben R; Jamoussi K; Mezghani-Jarraya R; van Pelt J; El-Feki A
[Ad] Endereço:Department of Life Sciences, Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Sfax , Sfax , Tunisia .
[Ti] Título:Protective effects of Hammada scoparia flavonoid-enriched fraction on liver injury induced by warm ischemia/reperfusion.
[So] Source:Pharm Biol;53(12):1810-7, 2015.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Hepatic ischemia/reperfusion injury (IRI) is a major cause of liver damage during liver surgery and transplantation. Plants have historically been used in treating liver damage, and Hammada scoparia (Pomel) (Chenopodiaceae) has been reported to possess a broad spectrum of pharmacological and therapeutic activities. OBJECTIVE: In this study, a flavonoid-enriched fraction was used before the warm ischemia (WI) process as pharmacological preconditioning and in combination with technical postconditioning to evaluate their protective effects. MATERIALS AND METHODS: The rats were divided into five groups: a sham group; a control group (Control-IR) that was submitted to 60 min WI; a Pharmacological Preconditioning group (PreC-IR) that received flavonoid-enriched fraction (200 mg/kg body weight); a Postconditioning group (PostC) and a PreC + PostC group. RESULTS: The use of the flavonoid-enriched fraction was noted to significantly (p < 0.05) reduce liver injury, as evidenced by the decrease in liver transaminase activities (AST and ALT) and lactic dehydrogenase (LDH), alkaline phosphatase (ALP), and lipid peroxidation (TBARS), levels as well as the enhancement of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) responses. The results also indicated that, compared with the separate application of pharmacological preconditioning and postconditioning, the combination of both treatments was more effective in reducing tissue oxidative stress levels through modulating SOD, GSH-PX, and CAT activities. Furthermore, the combined protocol further decreased the liver morphological score compared with solo treatment. DISCUSSION AND CONCLUSION: Overall, the results indicate that the H. scoparia flavonoid-enriched fraction could be a promising candidate for future application as a pharmacological preconditioning agent against hepatic IRI.
[Mh] Termos MeSH primário: Flavonoides/uso terapêutico
Fígado/irrigação sanguínea
Fígado/efeitos dos fármacos
Extratos Vegetais/uso terapêutico
Traumatismo por Reperfusão/prevenção & controle
Scoparia
Isquemia Quente/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Flavonoides/isolamento & purificação
Flavonoides/farmacologia
Fígado/metabolismo
Masculino
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Ratos
Ratos Wistar
Traumatismo por Reperfusão/metabolismo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150730
[Lr] Data última revisão:
150730
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150418
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2015.1010737


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[PMID]:25793965
[Au] Autor:Fuentes RG; Toume K; Arai MA; Sadhu SK; Ahmed F; Ishibashi M
[Ad] Endereço:†Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
[Ti] Título:Scopadulciol, Isolated from Scoparia dulcis, Induces ß-Catenin Degradation and Overcomes Tumor Necrosis Factor-Related Apoptosis Ligand Resistance in AGS Human Gastric Adenocarcinoma Cells.
[So] Source:J Nat Prod;78(4):864-72, 2015 Apr 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scopadulciol (1), a scopadulan-type diterpenoid, was isolated from Scoparia dulcis along with three other compounds (2-4) by an activity-guided approach using the TCF reporter (TOP) luciferase-based assay system. A fluorometric microculture cytotoxicity assay (FMCA) revealed that compound 1 was cytotoxic to AGS human gastric adenocarcinoma cells. The treatment of AGS cells with 1 decreased ß-catenin levels and also inhibited its nuclear localization. The pretreatment of AGS cells with a proteasome inhibitor, either MG132 or epoxomicin, protected against the degradation of ß-catenin induced by 1. The 1-induced degradation of ß-catenin was also abrogated in the presence of pifithrin-α, an inhibitor of p53 transcriptional activity. Compound 1 inhibited TOP activity in AGS cells and downregulated the protein levels of cyclin D1, c-myc, and survivin. Compound 1 also sensitized AGS cells to tumor necrosis factor-related apoptosis ligand (TRAIL)-induced apoptosis by increasing the levels of the death receptors, DR4 and DR5, and decreasing the level of the antiapoptotic protein Bcl-2. Collectively, our results demonstrated that 1 induced the p53- and proteasome-dependent degradation of ß-catenin, which resulted in the inhibition of TCF/ß-catenin transcription in AGS cells. Furthermore, 1 enhanced apoptosis in TRAIL-resistant AGS when combined with TRAIL.
[Mh] Termos MeSH primário: Diterpenos Abietanos/isolamento & purificação
Diterpenos Abietanos/farmacologia
Scoparia/química
[Mh] Termos MeSH secundário: Adenocarcinoma/tratamento farmacológico
Apoptose/efeitos dos fármacos
Benzotiazóis/farmacologia
Diterpenos Abietanos/química
Regulação para Baixo/efeitos dos fármacos
Seres Humanos
Leupeptinas/farmacologia
Estrutura Molecular
Receptores do Ligante Indutor de Apoptose Relacionado a TNF/efeitos dos fármacos
Receptores do Ligante Indutor de Apoptose Relacionado a TNF/genética
Neoplasias Gástricas/tratamento farmacológico
Ligante Indutor de Apoptose Relacionado a TNF/metabolismo
Tolueno/análogos & derivados
Tolueno/farmacologia
Fator de Necrose Tumoral alfa/metabolismo
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzothiazoles); 0 (Diterpenes, Abietane); 0 (Leupeptins); 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand); 0 (TNF-Related Apoptosis-Inducing Ligand); 0 (Tumor Necrosis Factor-alpha); 0 (beta Catenin); 136565-26-9 (scopadulciol); 3FPU23BG52 (Toluene); D213B92S1Y (pifithrin); RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150321
[St] Status:MEDLINE
[do] DOI:10.1021/np500933v


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[PMID]:25573588
[Au] Autor:Wankhar W; Srinivasan S; Rathinasamy S
[Ad] Endereço:a Department of Physiology , Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus , Chennai-600 113 , Tamilnadu , India.
[Ti] Título:HPTLC analysis of Scoparia dulcis Linn (Scrophulariaceae) and its larvicidal potential against dengue vector Aedes aegypti.
[So] Source:Nat Prod Res;29(18):1757-60, 2015.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study evaluates the larvicidal activity of Scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. The extract was tested at various concentrations ranging from 0.1 to 2 mg/mL against Aedes aegypti larvae. The extracts displayed significant larvicidal efficacy against Ae. aegypt species after 24 h exposure revealing LC50 of 3.3835 (mg/mL) and LC90 of 5.7578 (mg/mL). Finger printing profile carried out by CAMAG automatic TLC sample applicator programmed through WIN CATS software revealed peaks with different Rf values for three different volumes injected: 16, 15 and 18 peaks were spotted for 3, 6 and 9 µL, respectively. Ascending order of Rf values was also ascertained for each peak recorded. This study clearly signifies that S. dulcis extract contains numerous compounds that are known to have larvicidal properties which clearly substantiates its efficacy on Ae. aegypti larvae.
[Mh] Termos MeSH primário: Aedes
Inseticidas/química
Extratos Vegetais/química
Scoparia/química
[Mh] Termos MeSH secundário: Animais
Larva
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Plant Extracts)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150110
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2014.999060


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Yamamura, Y
Texto completo
[PMID]:25027024
[Au] Autor:Yamamura Y; Mizuguchi Y; Taura F; Kurosaki F
[Ad] Endereço:Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Sugitani, Toyama, 930-0194, Japan.
[Ti] Título:Transcriptional activation of a geranylgeranyl diphosphate synthase gene, GGPPS2, isolated from Scoparia dulcis by treatment with methyl jasmonate and yeast extract.
[So] Source:J Nat Med;68(4):748-53, 2014 Oct.
[Is] ISSN:1861-0293
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A cDNA clone, designated SdGGPPS2, was isolated from young seedlings of Scoparia dulcis. The putative amino acid sequence of the translate of the gene showed high homology with geranylgeranyl diphosphate synthase (GGPPS) from various plant sources, and the N-terminal residues exhibited the characteristics of chloroplast targeting sequence. An appreciable increase in the transcriptional level of SdGGPPS2 was observed by exposure of the leaf tissues of S. dulcis to methyl jasmonate, yeast extract or Ca(2+) ionophore A23187. In contrast, SdGGPPS1, a homologous GGPPS gene of the plant, showed no or only negligible change in the expression level upon treatment with these stimuli. The truncated protein heterologously expressed in Escherichia coli in which the putative targeting domain was deleted catalyzed the condensation of farnesyl diphosphate and isopentenyl diphosphate to liberate geranylgeranyl diphosphate. These results suggested that SdGGPPS2 plays physiological roles in methyl jasmonate and yeast extract-induced metabolism in the chloroplast of S. dulcis cells.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Ciclopentanos/farmacologia
Farnesiltranstransferase/genética
Oxilipinas/farmacologia
Scoparia/genética
Ativação Transcricional
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Farnesiltranstransferase/química
Farnesiltranstransferase/metabolismo
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Fosfatos de Poli-Isoprenil/metabolismo
Scoparia/efeitos dos fármacos
Scoparia/enzimologia
Alinhamento de Sequência
Sesquiterpenos/metabolismo
Leveduras
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (Oxylipins); 0 (Polyisoprenyl Phosphates); 0 (Sesquiterpenes); 79W6B01D07 (farnesyl pyrophosphate); 900N171A0F (methyl jasmonate); EC 2.5.1.29 (Farnesyltranstransferase); N21T0D88LX (geranylgeranyl pyrophosphate)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140717
[St] Status:MEDLINE
[do] DOI:10.1007/s11418-014-0855-7



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