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Pesquisa : B01.650.940.800.575.912.250.583.750 [Categoria DeCS]
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[PMID]:28638862
[Au] Autor:Song M; Chen Y; Du H; Zhang S; Wang Y; Zeng L; Yang J; Shi J; Wu Y; Wang D; Hu Y; Liu J
[Ad] Endereço:Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P R China.
[Ti] Título:RAW REHMANNIA RADIX POLYSACCHARIDE CAN EFFECTIVELY RELEASE PEROXIDATIVE INJURY INDUCED BY DUCK HEPATITIS A VIRUS.
[So] Source:Afr J Tradit Complement Altern Med;14(4):8-21, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH. MATERIALS AND METHODS: Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment. RESULTS: RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference ( <0.05) appeared between any two groups, demonstrating that it can alleviate liver pathological change. RRRP could make the hepatic injury evaluation indexes similar to BC group while the levels of the VC group were higher than other two groups in general. The levels of SOD, GSH-Px, CAT of RRRP group showed significant higher than that of VC group while the levels of NOS and MDA showed the opposite tendency, thus, RRRP could release peroxidative injury. CONCLUSION: RRRP was the most effective against duck hepatitis A virus (DHAV). RRRP could reduce mortality, alleviate liver pathological change, down-regulate liver lesion score, release peroxidative injury and hepatic injury. The antiviral and peroxidative injury releasing activity of RRRP for DHAV provided a platform to test novel drug strategies for hepatitis A virus in human beings.
[Mh] Termos MeSH primário: Antivirais/administração & dosagem
Vírus da Hepatite do Pato/efeitos dos fármacos
Hepatite Viral Animal/tratamento farmacológico
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/administração & dosagem
Polissacarídeos/administração & dosagem
Doenças das Aves Domésticas/tratamento farmacológico
Rehmannia/química
[Mh] Termos MeSH secundário: Animais
Astrágalo (Planta)/química
Patos
Vírus da Hepatite do Pato/fisiologia
Hepatite Viral Animal/diagnóstico por imagem
Hepatite Viral Animal/metabolismo
Hepatite Viral Animal/virologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/virologia
Raízes de Plantas/química
Doenças das Aves Domésticas/metabolismo
Doenças das Aves Domésticas/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Plant Extracts); 0 (Polysaccharides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i4.2


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[PMID]:28367097
[Au] Autor:Liu Y; Tang Q; Shao S; Chen Y; Chen W; Xu X
[Ad] Endereço:College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing 400715, China; Institute of Chinese Medicine, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Centre for Pharmacological Evaluation, Chongqing 400715, China.
[Ti] Título:Lyophilized Powder of Catalpol and Puerarin Protected Cerebral Vessels from Ischemia by Its Anti-apoptosis on Endothelial Cells.
[So] Source:Int J Biol Sci;13(3):327-338, 2017.
[Is] ISSN:1449-2288
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Catalpol and puerarin are two monomers of and , which are two herbs commonly used together in ancient prescriptions of traditional Chinese medicine for cerebral ischemia. Our previous study shows that the lyophilized powder of the two monomers improved the outcome of cerebral ischemia excellently in rodents. However, if it protects vessels from ischemia is unknown. The present research studied the protection of lyophilized powder of catalpol and puerarin (CP) on endothelial cells and the relative mechanism and . Middle cerebral artery occlusion (MCAO) rats were used to study the improvement of CP on neurological deficiency, regional cerebral blood flow (rCBF), and infarct volume. The morphology of vessels and the apoptosis of brain vascular endothelial cells (BVECs) were observed and detected by immunohistochemistry approaches. To study how CP protected primary BVECs (pBVECs) from ischemic penumbra, oxygen glucose deprivation (OGD)-damaged pBVECs were cultured in the condition of insufficient nutrition and low oxygen which recapitulate the low perfusion of ischemic penumbra. Using the cell model, the mechanism by which CP protected pBVECs was studied by shRNA and pathway inhibitors. CP at the dose of 65.4 mg/kg increased regional cerebral blood flow (rCBF), reduced infarct volume, protected vessel integrity and inhibited endothelial cell apoptosis . But it only improved rCBF, vessel integrity and BVECs apoptosis at the dose of 32.7 mg/kg. , the protection of CP on pBVECs was proved to be ERK/HIF-1a- and PI3K/AKT/mTOR/HIF-1a-dependent. This study indicates a possibility of CP being a new drug for cerebral ischemia. Besides, this research provides an alternative cell model for penumbra ECs study.
[Mh] Termos MeSH primário: Isquemia Encefálica/tratamento farmacológico
Glucosídeos Iridoides/farmacologia
Glucosídeos Iridoides/uso terapêutico
Isoflavonas/farmacologia
Isoflavonas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Western Blotting
Isquemia Encefálica/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Células Endoteliais/citologia
Células Endoteliais/efeitos dos fármacos
Glucose/metabolismo
Infarto da Artéria Cerebral Média/tratamento farmacológico
Infarto da Artéria Cerebral Média/metabolismo
Glucosídeos Iridoides/química
Isoflavonas/química
Masculino
Fármacos Neuroprotetores/química
Fármacos Neuroprotetores/farmacologia
Fármacos Neuroprotetores/uso terapêutico
Oxigênio/metabolismo
RNA Interferente Pequeno/genética
Ratos
Rehmannia/citologia
Rehmannia/efeitos dos fármacos
Rehmannia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iridoid Glucosides); 0 (Isoflavones); 0 (Neuroprotective Agents); 0 (RNA, Small Interfering); 2415-24-9 (catalpol); IY9XDZ35W2 (Glucose); S88TT14065 (Oxygen); Z9W8997416 (puerarin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.7150/ijbs.17751


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[PMID]:28111216
[Au] Autor:Liu C; Ma R; Wang L; Zhu R; Liu H; Guo Y; Zhao B; Zhao S; Tang J; Li Y; Niu J; Fu M; Zhang D; Gao S
[Ad] Endereço:Chinese Material Medica School, Beijing University of Chinese Medicine, Beijing 100102, China.
[Ti] Título:Rehmanniae Radix in osteoporosis: A review of traditional Chinese medicinal uses, phytochemistry, pharmacokinetics and pharmacology.
[So] Source:J Ethnopharmacol;198:351-362, 2017 Feb 23.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Emerging clinical usage and pharmacological effects have been achieved in using Rehmanniae Radix either singly or in combination with other herbs to treat skeletal diseases in traditional Chinese medicine (TCM) in the recent years. This study is aimed to provide a comprehensive review about the historical TCM interpretation of the action of Rehmanniae Radix in osteoporosis, its usage in clinical trials and osteoporotic models, its main phytochemical constituents, and its pharmacokinetics. MATERIALS AND METHODS: Several databases included PubMed, China Knowledge Resource Integrated Database, China Science and Technology Journal Database, National Science and Technology Library and the Web of Science Database were consulted to locate the publications pertaining to Rehmanniae Radix. The initial inquiry was conducted for the presence of the following terms combinations in the abstracts: Rehmanniae Radix, Dihuang, phytochemistry, pharmacokinetics, osteoporosis, bone, osteoclast and osteoblast. About 330 research papers and reviews were consulted. RESULTS: In TCM, Rehmanniae Radix exerts the anti-osteoporotic effect via regulating the functions of kidney and liver as well as improving blood circulation. 107 clinical trials are identified that used Rehmanniae Radix in combination with other herbs to treat post-menopausal, senile and secondary osteoporosis. Most of the clinical trials are characterized by high efficacy and no obvious adverse effects. However, the efficacies of these clinical trials are limited because of small patient sample size, short treatment duration and poor clinical design. In addition, TCM herbs under the clinical study are not clear because of a lack of standardization and authentication. The pharmacokinetics data demonstrate that the ingredients of Rehmanniae Radix are widely distributed after administration, and that catalpol and ajugol as well as acetoside are supposed to be the active constituents. More than 140 individual compounds have been currently isolated from this plant and reported to show pleiotropic effects on various diseases. Rehmanniae Radix displays bone protecting features in the osteoporosis models via the delicate balance between osteoclastogenesis and osteoblastogenesis through single herb extracts and its isolated compounds. CONCLUSIONS: The successful inclusion of Rehmanniae Radix in clinical trials and preclinical studies for the management of osteoporosis has attracted rising attentions for identifying potential anti-osteoporotic candidates from this plant and clinical existing TCM formulas, which will further speed up anti-osteoporosis drug discovery processes. Properly designed and well controlled prospective studies are still needed to further demonstrate bone protective actions and safe use of this herb and its ingredients.
[Mh] Termos MeSH primário: Osteoporose/tratamento farmacológico
Extratos Vegetais/farmacologia
Rehmannia/química
[Mh] Termos MeSH secundário: Animais
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacocinética
Medicamentos de Ervas Chinesas/farmacologia
Seres Humanos
Medicina Tradicional Chinesa/métodos
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Osteogênese/efeitos dos fármacos
Fitoterapia/métodos
Extratos Vegetais/química
Extratos Vegetais/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Plant Extracts)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


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[PMID]:27902928
[Au] Autor:Xu P; Su S; Tan C; Lai RS; Min ZS
[Ad] Endereço:First Clinical College, Nanjing University of Chinese Medicine, Nanjing 210029, China; Department of Dermatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210009, China.
[Ti] Título:Effects of aqueous extracts of Ecliptae herba, Polygoni multiflori radix praeparata and Rehmanniae radix praeparata on melanogenesis and the migration of human melanocytes.
[So] Source:J Ethnopharmacol;195:89-95, 2017 Jan 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Polygoni multiflori radix praeparata (PMRP), Ecliptae herba (EH) and Rehmanniae radix praeparata (RRP) are the most frequently-used herbs by Traditional Chinese Medicine practitioners for the treatment of vitiligo. Their abilities to stimulate melanogenesis, melanocyte migration and MITF (microphthalmia associated transcription factor) protein expression were evaluated in this study. MATERIALS AND METHODS: The effects of aqueous extracts of PMRP, EH and RRP on human melanocytes in vitro were examined by MTT assay, tyrosinase activity, melanin synthesis, migration assay and Western blot. RESULTS: Treatment with EH (at 100µg/ml and 400µg/ml) significantly increased intracellular tyrosinase activity in accordance with the elevation of melanin content at the same concentrations. Treatment with RRP (at 100µg/ml and 400µg/ml) promoted melanin production but had no stimulatory effect on tyrosinase activity. Treatment with PMRP and EH (at 100µg/ml) promoted the migration of human melanocytes in a type IV collagen-coated transwell migration assay. Western blot analysis showed MITF protein expression was elevated by PMRP, EH and RRP (at 100µg/ml). CONCLUSION: An aqueous extract of EH has a synergistic effect on melanocytes by up-regulating tyrosinase activity, enhancing melanin synthesis and promoting melanocyte migration as well as elevating MITF protein expression. RRP exhibits a significant stimulating effect on melanogenesis and MITF protein expression. These results suggest that EH and RRP contain substances with direct enhancing effects on melanogenesis and migration, possibly via their effects on MITF protein expression.
[Mh] Termos MeSH primário: Movimento Celular/efeitos dos fármacos
Eclipta/química
Melaninas/biossíntese
Melanócitos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Polygonaceae/química
Rehmannia/química
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Seres Humanos
Masculino
Melanócitos/metabolismo
Fator de Transcrição Associado à Microftalmia/metabolismo
Monofenol Mono-Oxigenase/metabolismo
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MITF protein, human); 0 (Melanins); 0 (Microphthalmia-Associated Transcription Factor); 0 (Plant Extracts); EC 1.14.18.1 (Monophenol Monooxygenase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE


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[PMID]:27873030
[Au] Autor:Kaneko S; Matsuki Y; Qiu YX; Isagi Y
[Ad] Endereço:Faculty of Symbiotic Systems Science, Fukushima University, 1 Kanayagawa, Fukushima, Fukushima, 960-1296, Japan. kaneko.shingo@gmail.com.
[Ti] Título:Chloroplast DNA sequencing and detailed microsatellite genotyping of all remnant populations suggests that only a single genet survives of the critically endangered plant Rehmannia japonica.
[So] Source:J Plant Res;130(1):117-124, 2017 Jan.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Rehmannia japonica (Thunb.) Makino ex T. Yamaz. is an endangered perennial herb species in Japan. Although earlier the Japanese considered it a variety of R. glutinosa, recent Japanese taxonomists have consistently regarded it as an independent species. According to the historical literature, Rehmannia japonica seems to have been known in China and Japan in the past. However, Chinese taxonomists do not recognize R. japonica at present. In Japan, only two populations are known, and although these populations flower every year, seed reproduction has not been observed. In this study, we aimed to reveal the phylogenetic relationships and levels of genetic diversity of R. japonica. A haplotype network based on two chloroplast DNA regions (trnL-trnF and rps16) showed that the sequences of R. japonica were distinguishable by three or four sites of indels from the most closely related species, R. chingii, consistent with the separate species status of R. japonica. An analysis of genetic diversity using twelve microsatellite loci showed that all of the ramets of R. japonica collected from two geographically isolated populations had an identical multilocus genotype, including identical heterozygous genotypes at six loci. This result indicated asexual origin of all sampled ramets. This study also suggests that the absence of sexual reproduction of R. japonica is explained by self-incompatibility combined with only a single genet remaining in the R. japonica populations.
[Mh] Termos MeSH primário: Variação Genética
Repetições de Microssatélites/genética
Rehmannia/genética
[Mh] Termos MeSH secundário: Animais
Cloroplastos/genética
Conservação dos Recursos Naturais
DNA de Cloroplastos/química
DNA de Cloroplastos/genética
Espécies em Perigo de Extinção
Genética Populacional
Genótipo
Haplótipos
Japão
Filogenia
Rehmannia/crescimento & desenvolvimento
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-016-0873-5


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[PMID]:28010833
[Au] Autor:Kim YJ; Lee CH; Kim JU; Yook TH
[Ad] Endereço:Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Woosuk University, Jeonju, South Korea.
[Ti] Título:Effects of Distilled Cervi Pantotrichum Cornu and Rehmannia glutinosa Pharmacopuncture at GB21 (Jianjing) on Heart Rate Variability: A Randomized and Double-blind Clinical Trial.
[So] Source:J Acupunct Meridian Stud;9(6):311-318, 2016 Dec.
[Is] ISSN:2093-8152
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/PURPOSE: The purpose of this study was to use heart rate variability (HRV) to investigate the effects of distilled Cervi Pantotrichum Cornu pharmacopuncture and Rehmannia glutinosa pharmacopuncture on the autonomic nervous system. MATERIALS AND METHODS: Forty healthy male participants were divided into two groups: the participants of the C-group received distilled Cervi Pantotrichum Cornu pharmacopuncture and those of the R-group received Rehmannia glutinosa pharmacopuncture. The study design was a randomized, double-blind clinical trial. Each participant received one of the two solutions injected at GB21 (Jianjing). The changes in HRV were measured seven times using the QECG-3: LXC3203 system (LAXTHA Inc. Korea). Time-dependent changes in HRV for each group were analyzed using the paired t test (significance level: p < 0.05), and the difference in the HRV fluctuations between the two experimental groups was evaluated using the independent sample test (significance level: p < 0.05). RESULTS AND CONCLUSION: The results showed that Cervi Pantotrichum Cornu pharmacopuncture and Rehmannia glutinosa pharmacopuncture tended to activate the autonomic nervous system within the normal range. Cervi Pantotrichum Cornu pharmacopuncture tended to activate the sympathetic nervous system, whereas Rehmannia glutinosa pharmacopuncture tended to activate both the sympathetic and parasympathetic nervous systems.
[Mh] Termos MeSH primário: Pontos de Acupuntura
Sistema Nervoso Autônomo/efeitos dos fármacos
Cornus/química
Frequência Cardíaca/efeitos dos fármacos
Extratos Vegetais/administração & dosagem
Rehmannia/química
[Mh] Termos MeSH secundário: Acupuntura
Sistema Nervoso Autônomo/fisiologia
Feminino
Seres Humanos
Injeções
Masculino
República da Coreia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE


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[PMID]:28008254
[Au] Autor:Huang Y; Qin T; Huang Y; Liu Z; Bo R; Hu Y; Liu J; Wu Y; Wang D
[Ad] Endereço:College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
[Ti] Título: polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells.
[So] Source:Int J Nanomedicine;11:6795-6808, 2016.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Nanomedicine, the medical application of nanotechnology, promises a seemingly limitless range of applications from drug delivery to adjuvants and therapeutics. Our current research is focused on natural polymer-based liposome adjuvants. With the aim of inducing protective and long-lasting immunity, the immunological adjuvant activity of polysaccharide liposome (RGPL) was investigated. In vivo, the splenic lymphocyte proliferation ratios and ovalbumin-specific immunoglobulin G titers of ovalbumin-RGPL-vaccinated mice were significantly upregulated. In draining lymph nodes, the expression of MHC II CD11c and CD86 CD11c was increased by RGPL; in addition, the percentages of central memory cells (T ) and effector memory cells (T ) were also elevated. RGPL could effectively provide adequate antigen exposure in lymph nodes. In vitro, RGPL could promote dendritic cell maturation and enhance dendritic cell functions, such as the mixed lymphocyte reaction and antigen presentation. Overall, the results demonstrated that RGPL has the potential to act as an effective controlled release vaccine adjuvant.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Apresentação do Antígeno/efeitos dos fármacos
Células Dendríticas/imunologia
Lipossomos/farmacologia
Polissacarídeos/farmacologia
Rehmannia/química
[Mh] Termos MeSH secundário: Animais
Antígeno B7-2/química
Antígeno CD11c/química
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Citocinas/química
Células Dendríticas/efeitos dos fármacos
Feminino
Imunoglobulina G/química
Imuno-Histoquímica
Memória Imunológica
Linfonodos/efeitos dos fármacos
Linfócitos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Ovalbumina/imunologia
Baço/efeitos dos fármacos
Linfócitos T/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (B7-2 Antigen); 0 (CD11c Antigen); 0 (Cytokines); 0 (Immunoglobulin G); 0 (Liposomes); 0 (Polysaccharides); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S119108


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[PMID]:27813551
[Au] Autor:Wang XH; Du HW; Guo XH; Wang SW; Zhou RB; Li Y; Li ZB; Zhao YS; Zhu QL
[Ad] Endereço:Department of Emergency, Military General Hospital of Beijing PLA, Beijing, China.
[Ti] Título:Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
[So] Source:Genet Mol Res;15(4), 2016 Oct 17.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to observe the effect of Rehmannia glutinosa oligosaccharide (RGO) on differentiation of bone marrow mesenchymal stem cells (MSCs) into cardiomyocyte-like cells . Rat MSCs were isolated, treated, and grouped as follows: RGO treatment group, 5-azacytidine (5-aza) treatment group, RGO + 5-aza treatment group, and control group. Following a four-week induction period, cardiac troponin I (cTnI) levels in MSCs were quantified by chemiluminescence, and the levels of myocardial enzymes creatine kinase (CK) and creatine kinase isoenzyme-MB (CK-MB) were measured using a dry chemistry analyzer. The cTnI- and connexin 43 (Cx43)-positive MSC population was identified by immunofluorescence, and expression levels of cTnI and Cx43 were analyzed by western blots. Following induction, cTnI, CK, and CK-MB levels were significantly higher in the RGO + 5-aza group as compared with the RGO and 5-aza groups (P < 0.05). In addition, fluorescence intensity of cTnI and Cx43 was higher in the RGO + 5-aza group as compared with the RGO and 5-aza groups. No cTnI- or Cx43-positive cells were detected in the control group. Western blot analysis further confirmed that cTnI and Cx43 were not expressed in the control group, while cTnI and Cx43 was higher in the RGO + 5-aza group than in the RGO and 5-aza groups. These results suggest that MSCs can be induced by RGO to differentiate into cardiomyocyte-like cells in vitro, and that RGO in combination with 5-aza enhance differentiation of MSCs.
[Mh] Termos MeSH primário: Células da Medula Óssea/citologia
Diferenciação Celular/efeitos dos fármacos
Células Mesenquimais Estromais/citologia
Miócitos Cardíacos/citologia
Oligossacarídeos/farmacologia
Rehmannia/química
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Western Blotting
Células da Medula Óssea/efeitos dos fármacos
Forma Celular/efeitos dos fármacos
Imunofluorescência
Masculino
Células Mesenquimais Estromais/efeitos dos fármacos
Miocárdio/metabolismo
Miócitos Cardíacos/efeitos dos fármacos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Oligosaccharides)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE
[do] DOI:10.4238/gmr15047795


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[PMID]:27618888
[Au] Autor:Teng Z; Shen Y; Li J; Lin Z; Chen M; Wang M; Li M; Dong H; Huang L
[Ad] Endereço:State Key Laboratory Breeding Base of Dao-Di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zhongqiuteng@163.com.
[Ti] Título:Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein.
[So] Source:Molecules;21(9), 2016 Aug 27.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a "top grade" herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).
[Mh] Termos MeSH primário: Proteínas do Capsídeo
Cucumovirus/genética
Plantas Geneticamente Modificadas
Rehmannia
Vírus do Mosaico do Tabaco/genética
[Mh] Termos MeSH secundário: Proteínas do Capsídeo/biossíntese
Proteínas do Capsídeo/genética
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Rehmannia/genética
Rehmannia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  10 / 265 MEDLINE  
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[PMID]:27180436
[Au] Autor:Piatczak E; Dfbska M; Kontek B; Olas B; Wysokinskai H
[Ti] Título:ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACTS FROM THE SHOOTS AND ROOTS OF pRi-TRANSFORMED PLANTS OF REHMANNIA GLUTINOSA LIBOSCH.
[So] Source:Acta Pol Pharm;73(2):433-8, 2016 Mar-Apr.
[Is] ISSN:0001-6837
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The antioxidant activity of methanolic extracts derived from shoots (HR-shoots) and roots (HR-roots) of pRi-transformed Rehmannia glutinosa plants were determined. The activity was indicated by the ability of the plant extracts to inhibit superoxide anion (O2(-·)) generation and thiobarbituric acid reactive substances (TBARS) production in resting blood platelets and platelets activated by thrombin. The strongest activity was exhibited by the HR-shoot extract (50 µg/mL). The present study also examines the antioxidant properties of the plant extracts against human plasma lipid peroxidation induced by strong biological oxidants: hydrogen peroxide (H2O2) and H2O2/Fe. The study shows that extracts from transformed R. glutinosa plants may be a promising source of natural antioxidants, which would be valuable in various cardiovascular diseases. The extracts may also protect lipids against oxidative modifications.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Metanol/química
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
Plantas Geneticamente Modificadas/genética
Rehmannia/genética
Solventes/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
Plaquetas/efeitos dos fármacos
Plaquetas/metabolismo
Feminino
Regulação da Expressão Gênica de Plantas
Seres Humanos
Peróxido de Hidrogênio/farmacologia
Peroxidação de Lipídeos/efeitos dos fármacos
Masculino
Oxidantes/farmacologia
Fitoterapia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Raízes de Plantas
Brotos de Planta
Plantas Geneticamente Modificadas/química
Plantas Medicinais
Rehmannia/química
Superóxidos/metabolismo
Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
Trombina/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Oxidants); 0 (Plant Extracts); 0 (Solvents); 0 (Thiobarbituric Acid Reactive Substances); 11062-77-4 (Superoxides); BBX060AN9V (Hydrogen Peroxide); EC 3.4.21.5 (Thrombin); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160516
[Lr] Data última revisão:
160516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160517
[St] Status:MEDLINE



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