Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.583.990.433 [Categoria DeCS]
Referências encontradas : 247 [refinar]
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[PMID]:28655616
[Au] Autor:Hurd MC; Kwon M; Ro DK
[Ad] Endereço:University of Calgary, Department of Biological Sciences, Calgary, AB, T2N 1N4, Canada.
[Ti] Título:Functional identification of a Lippia dulcis bornyl diphosphate synthase that contains a duplicated, inhibitory arginine-rich motif.
[So] Source:Biochem Biophys Res Commun;490(3):963-968, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lippia dulcis (Aztec sweet herb) contains the potent natural sweetener hernandulcin, a sesquiterpene ketone found in the leaves and flowers. Utilizing the leaves for agricultural application is challenging due to the presence of the bitter-tasting and toxic monoterpene, camphor. To unlock the commercial potential of L. dulcis leaves, the first step of camphor biosynthesis by a bornyl diphosphate synthase needs to be elucidated. Two putative monoterpene synthases (LdTPS3 and LdTPS9) were isolated from L. dulcis leaf cDNA. To elucidate their catalytic functions, E. coli-produced recombinant enzymes with truncations of their chloroplast transit peptides were assayed with geranyl diphosphate (GPP). In vitro enzyme assays showed that LdTPS3 encodes bornyl diphosphate synthase (thus named LdBPPS) while LdTPS9 encodes linalool synthase. Interestingly, the N-terminus of LdBPPS possesses two arginine-rich (RRX W) motifs, and enzyme assays showed that the presence of both RRX W motifs completely inhibits the catalytic activity of LdBPPS. Only after the removal of the putative chloroplast transit peptide and the first RRX W, LdBPPS could react with GPP to produce bornyl diphosphate. LdBPPS is distantly related to the known bornyl diphosphate synthase from sage in a phylogenetic analysis, indicating a converged evolution of camphor biosynthesis in sage and L. dulcis. The discovery of LdBPPS opens up the possibility of engineering L. dulcis to remove the undesirable product, camphor.
[Mh] Termos MeSH primário: Cânfora/metabolismo
Liases Intramoleculares/metabolismo
Lippia/enzimologia
Sesquiterpenos/metabolismo
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Arginina/química
Arginina/metabolismo
Liases Intramoleculares/química
Liases Intramoleculares/genética
Lippia/química
Lippia/genética
Lippia/metabolismo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sesquiterpenes); 76-22-2 (Camphor); 7V22TJL7NX (hernandulcin); 94ZLA3W45F (Arginine); EC 5.5.- (Intramolecular Lyases); EC 5.5.1.8 (geranyl-diphosphate cyclase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


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[PMID]:28415554
[Au] Autor:Elemike EE; Onwudiwe DC; Ekennia AC; Ehiri RC; Nnaji NJ
[Ad] Endereço:Material Science Innovation and Modelling (MaSIM) Research Focus Area, Faculty of Agriculture, Science and Technology, North-West University (Mafikeng Campus), Private Bag X2046, Mmabatho, South Africa; Department of Chemistry, School of Mathematical and Physical Sciences, North-West University (Maf
[Ti] Título:Phytosynthesis of silver nanoparticles using aqueous leaf extracts of Lippia citriodora: Antimicrobial, larvicidal and photocatalytic evaluations.
[So] Source:Mater Sci Eng C Mater Biol Appl;75:980-989, 2017 Jun 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nanoscience and nanotechnology represent new and enabling platforms that promise to provide broad range of novel and improved technologies for environmental, biological and other scientific applications. This study reports the synthesis of silver nanoparticles mediated by aqueous leaf extract of Lippia citriodora at two different temperatures of 50°C and 90°C. The synthesis of colloidal silver nanoparticles (AgNPs) was monitored by the use of UV-visible spectroscopy at different temperatures and time intervals. The surface plasmon bands (SPBs) showed peaks between 417 and 421nm at 90°C and around 430nm at 50°C, indicating a red shift at lower temperature. Fourier transform infrared (FTIR) analysis of the nanoparticles showed the presence of similar peaks found in the spectra of the plant extract. The size of the AgNPs was confirmed by transmission electron microscopy (TEM) which indicated an average size of 23.8nm (90°C) and 25nm (50°C). The nanoparticles showed better antimicrobial activities when compared to the crude plant extract against several screened pathogens: Gram negative (Escherichia coli, and Salmonella typhi) and Gram positive (Bacillus subtilis and Staphylococcus aureus) strains and a fungi organism; Candida albicans. In addition, the AgNPs showed good larvicidal efficacy against early 4th instar of Culex quinquefasciatus (a vector of lymphatic filariasis). Finally, the nanoparticles exhibited photocatalytic properties on an industrial waste pollutant, methylene blue.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Lippia/química
Nanopartículas Metálicas/química
Extratos Vegetais/química
Folhas de Planta/química
Prata/química
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/farmacologia
Bacillus subtilis/efeitos dos fármacos
Culicidae/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Fotoquímica
Extratos Vegetais/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Plant Extracts); 3M4G523W1G (Silver)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE


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[PMID]:28385098
[Au] Autor:González-Trujano ME; Hernández-Sánchez LY; Muñoz Ocotero V; Dorazco-González A; Guevara Fefer P; Aguirre-Hernández E
[Ad] Endereço:a Laboratorio de Neurofarmacología de Productos Naturales, Dirección de Investigaciones en Neurociencias , Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz , Mexico City , Mexico.
[Ti] Título:Pharmacological evaluation of the anxiolytic-like effects of Lippia graveolens and bioactive compounds.
[So] Source:Pharm Biol;55(1):1569-1576, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Lippia species (Verbenaceae) are widely used in Latin America and Africa as folk medicine for their tranquilizing properties. OBJECTIVE: To evaluate the anxiolytic-like effects and safety of Lippia graveolens Kunth. by exploring its aqueous and organic leaf extracts and identifying the responsible chemical constituents. MATERIAL AND METHODS: Aqueous and organic extracts (hexane, ethyl acetate and methanol) were pharmacologically evaluated at several doses. Chemical constituents were identified using MS, NMR and GC-MS analysis. The isolated compounds (3 mg/kg, i.p.), extracts (1, 3, 10 and 30 mg/kg, i.p.), and the reference drug diazepam (0.1 mg/kg, i.p.) were assessed in CD-1 mice using experimental behavioural models: open-field, cylinder, hole-board, plus-maze and sodium pentobarbital-induced hypnosis, as well as their acute toxicity (LD ). RESULTS: After administration of the extracts and bioactive compounds, a significant anxiolytic-like response from 1 mg/kg, i.p. was observed, resembling the effect of diazepam. Major presence of thymol (33.40%) was observed in the hexane extract; whereas for the first time in this species a p-cymene + thymol mixture (9.78%), naringenin (0.18%) and cirsimaritin (1.16%) were obtained as bioactive constituents of the ethyl acetate crude extract. Acute toxicity was calculated to be LD = 1000 mg/kg for the crude hexane extract, lower in comparison to the other extracts analyzed (LD > 2000 mg/kg). DISCUSSION AND CONCLUSION: Our results suggest that L. graveolens exerts anxiolytic-like activity involving many kinds of constituents, mainly of the terpenoid and flavonoid nature. These results reinforce the potential use of this species in the therapy of anxiety.
[Mh] Termos MeSH primário: Ansiolíticos/farmacologia
Ansiedade/prevenção & controle
Lippia/química
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Ansiolíticos/isolamento & purificação
Ansiolíticos/toxicidade
Ansiedade/psicologia
Comportamento Animal/efeitos dos fármacos
Diazepam/farmacologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Cromatografia Gasosa-Espectrometria de Massas
Dose Letal Mediana
Espectroscopia de Ressonância Magnética
Masculino
Aprendizagem em Labirinto/efeitos dos fármacos
Camundongos
Compostos Fitoquímicos/isolamento & purificação
Compostos Fitoquímicos/toxicidade
Fitoterapia
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/toxicidade
Folhas de Planta/química
Plantas Medicinais
Sono/efeitos dos fármacos
Solventes/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Anxiety Agents); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Solvents); Q3JTX2Q7TU (Diazepam)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1310908


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[PMID]:28317320
[Au] Autor:Oukerrou MA; Tilaoui M; Mouse HA; Bouchmaa N; Zyad A
[Ad] Endereço:Laboratory of Biological Engineering, Natural Substances, Cellular and Molecular Immuno-Pharmacology, Immunobiology of Cancer Cells Cluster, Faculty of Science and Technology, Sultan Moulay Slimane University, P.B 523, 23000, Beni Mellal, Morocco.
[Ti] Título:Differential Cytotoxic Activity of Essential Oil of Lippia citriodora from Different Regions in Morocco.
[So] Source:Chem Biodivers;14(7), 2017 Jul.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to investigate the cytotoxic effect of the essential oil of dried leaves of Lippia citriodora (H.B. & K.) harvested in different regions of Morocco. This effect was evaluated against the P815 murine mastocytoma cell line using the MTT assay. Interestingly, this work demonstrated for the first time that these essential oils exhibited a strong cytotoxic activity against the P815 cell line, with IC values ranging from 7.75 to 13.25 µg/ml. This cytotoxicity began early and increased in a dose- and time-dependent manner. The chemical profile of these essential oils was analyzed by gas chromatography coupled to mass spectrometry. Importantly, the difference in terms of major components' contents was not significant suggesting probably that the differential cytotoxicity between these essential oils could be attributed to the difference in the content of these essential oils in minor compounds, which could interact with each other or with the main molecules. Finally, this study demonstrated for the first time that essential oils of L. citriodora from different regions in Morocco induced apoptosis against P815 tumor cell line.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Lippia/química
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Linhagem Celular Tumoral
Cromatografia Gasosa-Espectrometria de Massas
Concentração Inibidora 50
Camundongos
Marrocos
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Oils, Volatile)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201600497


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[PMID]:28115182
[Au] Autor:Soares BV; Neves LR; Ferreira DO; Oliveira MS; Chaves FC; Chagas EC; Gonçalves RA; Tavares-Dias M
[Ad] Endereço:Programa de Pós-Graduação em Biodiversidade Tropical (PPGBIO), Universidade Federal do Amapá (UNIFAP), Macapá, AP, Brazil.
[Ti] Título:Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae).
[So] Source:Vet Parasitol;234:49-56, 2017 Jan 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160mg/L were 100% effective against monogenoideans in 10min and 1h of exposure, respectively. However, the effectiveness of 100% concentrations of 80mg/L and 40mg/L occurred in 3 and 6h, respectively. In the in vivo tests, juvenile fish were submitted to 60min of baths with 10mg/L and 15min of baths with 20mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites.
[Mh] Termos MeSH primário: Caraciformes/parasitologia
Doenças dos Peixes/tratamento farmacológico
Lippia/química
Óleos Voláteis/farmacologia
Óleos Voláteis/uso terapêutico
Trematódeos/efeitos dos fármacos
Infecções por Trematódeos/veterinária
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/farmacologia
Anti-Helmínticos/uso terapêutico
Anti-Helmínticos/toxicidade
Eritrócitos/efeitos dos fármacos
Doenças dos Peixes/parasitologia
Brânquias/efeitos dos fármacos
Brânquias/parasitologia
Óleos Voláteis/toxicidade
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Extratos Vegetais/toxicidade
Infecções por Trematódeos/tratamento farmacológico
Infecções por Trematódeos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Oils, Volatile); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


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[PMID]:28055127
[Au] Autor:Hernandes C; Pina ES; Taleb-Contini SH; Bertoni BW; Cestari IM; Espanha LG; Varanda EA; Camilo KF; Martinez EZ; França SC; Pereira AM
[Ad] Endereço:Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
[Ti] Título:Lippia origanoides essential oil: an efficient and safe alternative to preserve food, cosmetic and pharmaceutical products.
[So] Source:J Appl Microbiol;122(4):900-910, 2017 Apr.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The aim of this work was to evaluate the efficacy and safety of Lippia origanoides essential oil as a preservative in industrial products. METHODS AND RESULTS: The composition, antimicrobial activity, mutagenic and toxic potential of L. origanoides were determined. Then, the effect of essential oil as a preservative in food, cosmetics and pharmaceutical products was evaluated. The essential oil of L. origanoides consisted mainly of oxygenated monoterpenes (38·13%); 26·28% corresponded to the compound carvacrol. At concentrations ranging from 0·312 to 1·25 µl ml and in association with polysorbate 80, the essential oil of L. origanoides inhibited the growth of all the tested micro-organisms. The medium lethal dose in mice was 3·5 g kg , which categorizes it as nontoxic according to the European Union criteria, and negative results in the Ames test indicated that this oil was not mutagenic. In combination with polysorbate 80, the essential oil exerted preservative action on orange juice, cosmetic and pharmaceutical compositions, especially in the case of aqueous-based products. CONCLUSIONS: Lippia origanoides essential oil is an effective and safe preservative for orange juice, pharmaceutical and cosmetic products. SIGNIFICANCE AND IMPACT OF THE STUDY: This study allowed for the complete understanding of the antimicrobial action and toxicological potential of L. origanoides essential oil. These results facilitate the development of a preservative system based on L. origanoides essential oil.
[Mh] Termos MeSH primário: Cosméticos
Conservantes de Alimentos/farmacologia
Lippia/química
Óleos Voláteis/farmacologia
Conservantes Farmacêuticos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Conservantes de Alimentos/química
Conservantes de Alimentos/toxicidade
Camundongos
Monoterpenos/química
Óleos Voláteis/química
Óleos Voláteis/toxicidade
Excipientes Farmacêuticos/química
Excipientes Farmacêuticos/farmacologia
Excipientes Farmacêuticos/toxicidade
Óleos Vegetais/química
Óleos Vegetais/farmacologia
Óleos Vegetais/toxicidade
Conservantes Farmacêuticos/química
Conservantes Farmacêuticos/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cosmetics); 0 (Food Preservatives); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Pharmaceutic Aids); 0 (Plant Oils); 0 (Preservatives, Pharmaceutical); 9B1J4V995Q (carvacrol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13398


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[PMID]:28039793
[Au] Autor:García-Bores AM; Espinosa-González AM; Reyna-Campos A; Cruz-Toscano S; Benítez-Flores JC; Hernández-Delgado CT; Flores-Maya S; Urzúa-Meza M; Peñalosa-Castro I; Céspedes-Acuña CL; Avila-Acevedo JG
[Ad] Endereço:Laboratorio de Fitoquímica, UBIPRO, FES-Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla, Edo. de México C.P. 54090, Mexico. Electronic address: boresana@yahoo.com.
[Ti] Título:Lippia graveolens photochemopreventive effect against UVB radiation-induced skin carcinogenesis.
[So] Source:J Photochem Photobiol B;167:72-81, 2017 Feb.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Lippia graveolens HBK (Mexican oregano) is a species that is regularly used as a condiment in Mexican cuisine. In traditional medicine, it is used for the treatment of respiratory and digestive illnesses, headaches, rheumatism and inflammation-related disorders. The main chemical components reported in this species include the following: terpenoids, iridoids and flavonoids. The aim of this study was to determine the potential photochemopreventive effect of the methanolic extract of Lippia graveolens (MELG) against ultraviolet B (UVB)-induced skin cancer in SKH-1 mice. The phenolic content, radical scavenger activity, penetration and genotoxicity of the MELG were also evaluated. The MELG exhibited scavenging activity against 1,1-diphenyl-2-picrylhydrazyl, superoxide and hydroxyl radicals, and it did not exhibit genotoxic activity in the micronucleus test. In addition, the MELG absorbed UVB (280nm) electromagnetic radiation. The main components detected in the plant extract were naringenin and galangin, and pinocembrin was also isolated and identified through spectroscopic analysis. The MELG demonstrated a photoprotective effect against UVB-induced cell death in Escherichia coli. In chronic challenge experiments, the MELG protected against UVB-induced skin cancer in SKH-1 mice. The MELG penetrated the skin of mice. Topical administration of the MELG protected against chronic UVB-induced damage in mouse SKH-1 skin. Our results suggest that the MELG has photochemopreventive activity and may potentially prevent photo-tumorigenesis.
[Mh] Termos MeSH primário: Lippia/química
Neoplasias Induzidas por Radiação/prevenção & controle
Extratos Vegetais/farmacologia
Protetores contra Radiação/farmacologia
Neoplasias Cutâneas/prevenção & controle
Raios Ultravioleta
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Feminino
Camundongos
Camundongos Pelados
Testes de Mutagenicidade
Análise Espectral/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Radiation-Protective Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE


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[PMID]:27905068
[Au] Autor:Montero-Villegas S; Polo M; Galle M; Rodenak-Kladniew B; Castro M; Ves-Losada A; Crespo R; García de Bravo M
[Ad] Endereço:INIBIOLP "Prof. Dr. Rodolfo R. Brenner" (CONICET-CCT La Plata), Facultad Ciencias Médicas, UNLP, Calles 60 y 120, La Plata, Argentina.
[Ti] Título:Inhibition of Mevalonate Pathway and Synthesis of the Storage Lipids in Human Liver-Derived and Non-liver Cell Lines by Lippia alba Essential Oils.
[So] Source:Lipids;52(1):37-49, 2017 Jan.
[Is] ISSN:1558-9307
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The essential oils (EOs) of Lippia alba, an herb extensively used as a folk medicine in Latin America, are today promoted as an effective means of eliminating problems caused by hyperlipemia. We hypothesized that L.alba EOs inhibited cholesterol and triacylglycerols synthesis and decreased the intracellular depots of those lipids (lipid droplets), mechanisms involving the induction of a hypolipidemic response. Our aim was, therefore, to evaluate the hypolipogenic capability of the EOs of four L. alba chemotypes on liver-derived (HepG2) and non-liver (A549) human cell lines and to identify the potential biochemical targets of those chemotypes, particularly within the mevalonate pathway (MP). [ C]Acetate was used as radioactive precursor for assays. Lipid analyses were performed by thin-layer and capillary gas chromatography, lipid droplets analyzed by fluorescence microscopy, and HMGCR levels determined by Western blot. In both cell lines, all four chemotypes exerted hypocholesterogenic effects within a concentration range of 3.2-32 µg/mL. Nonsaponifiable lipids manifested a decrease in incorporation of [ C]acetate into squalene, lanosterol, lathosterol, and cholesterol, but not into ubiquinone, thus suggesting an inhibition of enzymes in the MP downstream from farnesyl pyrophosphate. The tagetenone chemotype, the most efficacious hypocholesterogenic L. alba EO, lowered HMGCR protein levels; inhibited triacylglycerols, cholesteryl esters, and phospholipids synthesis; and diminished lipid droplets in size and volume. These results revealed that L. alba EOs inhibited different lipogenic pathways and such lipid-lowering effects could prove essential to prevent cardiovascular diseases.
[Mh] Termos MeSH primário: Vias Biossintéticas/efeitos dos fármacos
Lippia/química
Ácido Mevalônico/metabolismo
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Células A549
Linhagem Celular
Colesterol/biossíntese
Células Hep G2
Seres Humanos
Óleos Vegetais/farmacologia
Triglicerídeos/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (Plant Oils); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol); S5UOB36OCZ (Mevalonic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1007/s11745-016-4218-x


  9 / 247 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:27878214
[Au] Autor:Figueiredo MB; Gomes GA; Santangelo JM; Pontes EG; Azambuja P; Garcia ES; Carvalho MG
[Ad] Endereço:Universidade Federal Rural do Rio de Janeiro, Instituto de Ciências Exatas, Departamento de Química, Seropédica, RJ, Brasil.
[Ti] Título:Lethal and sublethal effects of essential oil of Lippia sidoides (Verbenaceae) and monoterpenes on Chagas' disease vector Rhodnius prolixus.
[So] Source:Mem Inst Oswaldo Cruz;112(1):63-69, 2017 Jan 01.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to identify the composition of the essential oil from leaves of Lippia sidoides (EOLS), a typical shrub commonly found in the dry northeast of Brazil, popularly known as "alecrim-pimenta". Additionally, we investigated the nymphicidal, ovicidal, phagoinhibitory and excretion effects of EOLS, its major constituent thymol and its isomer carvacrol, on fourth instar nymphs and eggs of Rhodnius prolixus, the Chagas' disease vector. The nymphicidal and ovicidal activity of thymol, carvacrol, and EOLS was assessed by tests using impregnated Petri dishes. The lethal concentration values (LC50) for EOLS, carvacrol, and thymol were 54.48, 32.98, and 9.38 mg/cm2, respectively. The ovicidal test showed that both carvacrol and thymol (50 mg/cm2) inhibited hatching (50% and 23.3%, respectively), while treatments with 10 mg/cm2 or 50 mg/cm2 EOLS did not affect the hatching rate at all (80% and 90%, respectively). We observed an anti-feeding effect in insects fed with blood containing natural products at the higher concentrations (100 µg/mL). Finally, excretion rate was affected by EOLS and carvacrol, but not by thymol. These findings offer novel insights into basic physiological processes that make the tested natural compounds interesting candidates for new types of insecticides.
[Mh] Termos MeSH primário: Lippia/química
Monoterpenos/administração & dosagem
Óleos Voláteis/administração & dosagem
Rhodnius/efeitos dos fármacos
Timol/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Doença de Chagas/transmissão
Relação Dose-Resposta a Droga
Dose Letal Mediana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Oils, Volatile); 3J50XA376E (Thymol); 9B1J4V995Q (carvacrol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


  10 / 247 MEDLINE  
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[PMID]:27653530
[Au] Autor:Nemati Kharajuo M; Piryaei M; Babashpour Asl M; Abolghasemi MM
[Ad] Endereço:a Department of Horticulture, College of Agriculture , Islamic Azad University , Maragheh , Iran.
[Ti] Título:Fast analysis of volatile compounds from Lippia citriodora with nanoporous aluminum wire as solid-phase microextraction fibres.
[So] Source:Nat Prod Res;31(3):351-354, 2017 Feb.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, the efficiency of nanoporous aluminium wires as fibres for headspace solid-phase microextraction (HS-SPME) of volatile compounds from Lippia citriodora was investigated and compared with two anodised methods. The prepared fibres are durable with very good chemical and thermal stability which can be coupled to GC and GC/MS. A one at-a-time optimisation strategy was applied for optimising the important extraction parameters such as extraction temperature, extraction time, sample mass and added water. Compared with hydrodistillation (HD), HS-SPME provide the advantages of a small amount of sample, time-saving, simplicity and cheapness.
[Mh] Termos MeSH primário: Alumínio/química
Lippia/química
Microextração em Fase Sólida/métodos
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas/métodos
Nanoporos
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds); CPD4NFA903 (Aluminum)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE



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