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Pesquisa : B01.650.940.800.575.912.250.618.050.750.088 [Categoria DeCS]
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[PMID]:27914529
[Au] Autor:Feng LX; Sun P; Mi T; Liu M; Liu W; Yao S; Cao YM; Yu XL; Wu WY; Jiang BH; Yang M; Guo DA; Liu X
[Ad] Endereço:Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
[Ti] Título:Agglutinin isolated from Arisema heterophyllum Blume induces apoptosis and autophagy in A549 cells through inhibiting PI3K/Akt pathway and inducing ER stress.
[So] Source:Chin J Nat Med;14(11):856-864, 2016 Nov.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis (RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the underlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume (AHA) and explore the possible mechanisms in human non-small-cell lung cancer A549 cells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction chromatography. AHA dose-dependently inhibited the proliferation of A549 cells and induced G phase cell cycle arrest. AHA induced apoptosis by up-regulating pro-apoptotic Bax, decreasing anti-apoptotic Bcl-2, and activating caspase-9 and caspase-3. In A549 cells treated with AHA, the PI3K/Akt pathway was inhibited. Furthermore, AHA induced increase in the levels of ER stress markers such as phosphorylated eukaryotic initiation factor 2α (p-eIF2α), C/EBP-homologous protein (CHOP), inositol-requiring enzyme 1α (IRE1α), and phosphorylated c-Jun NH -terminal kinase (p-JNK). AHA also induced autophagy in A549 cells. Staining of acidic vesicular organelles (AVOs) and increase in the levels of LC3II and ATG7 were observed in AHA-treated cells. These findings suggested that AHA might be one of the active components with anti-cancer effects in Arisaema heterophyllum Blume. In conclusion, cytotoxicity of AHA on cancer cells might be related to its effects on apoptosis and autophagy through inhibition of PI3K/Akt pathway and induction of ER stress.
[Mh] Termos MeSH primário: Aglutininas/farmacologia
Apoptose/efeitos dos fármacos
Arisaema/química
Autofagia/efeitos dos fármacos
Carcinoma Pulmonar de Células não Pequenas/fisiopatologia
Medicamentos de Ervas Chinesas/farmacologia
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
[Mh] Termos MeSH secundário: Células A549
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico
Carcinoma Pulmonar de Células não Pequenas/enzimologia
Carcinoma Pulmonar de Células não Pequenas/metabolismo
Linhagem Celular Tumoral
Seres Humanos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Fosfatidilinositol 3-Quinases/genética
Fosforilação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agglutinins); 0 (Drugs, Chinese Herbal); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27166544
[Au] Autor:Azam S; Saqib MS; Zar F; Ahmad B; Khan I; Zeb Z; Khan I
[Ad] Endereço:Centre of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
[Ti] Título:Antibacterial, antifungal, phytotoxic, antioxidant and hemagglutination activities of organic fractions of Arisaema tortuosum.
[So] Source:Pak J Pharm Sci;29(3):991-7, 2016 May.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:In the current study, the antimicrobial, phytotoxic, haemagglutination and antioxidant potential of crude methanolic extract (Crd. MeOH Ext.) and four organic fractions of Arisaema tortuosum was investigated. All fractions have been screened for antimicrobial properties against eight bacterial pathogens and six fungal pathogens using agar well diffusion and tube dilution method, respectively. Furthermore, the organic fractions were also screened for its phytotoxicity against Lemna minor. Haemagglutination was performed against all human blood groups while free radical scavenging activity was performed to investigate the antioxidant potential of A. tortuosum. Results obtained for antibacterial activity exhibited various degree of zone of inhibition and significant activity was observed for Pseudomonas aeruginosa (27.16±0.60) followed by Bacillus cereus (18.55±0.69) for Crd. MeOH Ext. and chloroform (CHCl3) fraction, respectively while some strains showed resistant at same concentration. Similarly, non-significant antifungal activity was observed for the plant extracts. However, the highest activity among the strains was observed for Alternaria alternata (22±1.24%) and Aspergillus niger (20±1.00%) for ethyl acetate (EtOAc) fraction and Crd. MeOH Ext., respectively. The plant extracts showed good phytotoxic activity with 77.06% inhibition for n-hexane fraction at 1000µg/mL. The result of Nitric Oxide (NO) reducing assay revealed that the plant has less antioxidant activity with 46.06% inhibition for CHCl(3) fraction at 900µg/mL. For haemagglutination assay, the result displayed no agglutination in all the testing concentration. Based on the current results, it can be concluded that A. tortuosum has significant antimicrobial and moderate phytotoxic potential and therefore can leads to antibiotics and herbicide production.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antifúngicos/farmacologia
Antioxidantes/farmacologia
Arisaema/química
Hemaglutinação/efeitos dos fármacos
Herbicidas/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/isolamento & purificação
Antifúngicos/isolamento & purificação
Antioxidantes/isolamento & purificação
Fracionamento Químico
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Farmacorresistência Bacteriana
Testes de Hemaglutinação
Herbicidas/isolamento & purificação
Seres Humanos
Óxido Nítrico/química
Oxirredução
Fitoterapia
Componentes Aéreos da Planta
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Rizoma
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Antioxidants); 0 (Herbicides); 0 (Plant Extracts); 0 (Solvents); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160512
[St] Status:MEDLINE


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[PMID]:26909979
[Au] Autor:Moon BC; Kim WJ; Ji Y; Lee YM; Kang YM; Choi G
[Ad] Endereço:K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
[Ti] Título:Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences.
[So] Source:Genet Mol Res;15(1), 2016 Feb 19.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional methods. Therefore, we analyzed DNA barcode sequences to identify each herbal medicine and the common adulterants at a species level. To verify the identity of these herbal medicines, we collected five authentic species (Pinellia ternata for Pinelliae Tuber, and Arisaema amurense, A. amurense var. serratum, A. erubescens, and A. heterophyllum for Arisaematis Rhizoma) and six common adulterant plant species. Maturase K (matK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) genes were then amplified using universal primers. In comparative analyses of two DNA barcode sequences, we obtained 45 species-specific nucleotides sufficient to identify each species (except A. erubescens with matK) and 28 marker nucleotides for each species (except P. pedatisecta with rbcL). Sequence differences at corresponding positions of the two combined DNA barcodes provided genetic marker nucleotides that could be used to identify specimens of the correct species among the analyzed medicinal plants. Furthermore, we generated a phylogenetic tree showing nine distinct groups depending on the species. These results can be used to authenticate Pinelliae Tuber and Arisaematis Rhizoma from their adulterants and to identify each species. Thus, comparative analyses of plant DNA barcode sequences identified useful genetic markers for the authentication of Pinelliae Tuber and Arisaematis Rhizoma from several adulterant herbal materials.
[Mh] Termos MeSH primário: Arisaema/genética
Código de Barras de DNA Taxonômico
Genes de Plantas
Pinellia/genética
Plantas Medicinais/genética
[Mh] Termos MeSH secundário: Arisaema/classificação
Sequência de Bases
Dados de Sequência Molecular
Filogenia
Pinellia/classificação
Plantas Medicinais/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15017064


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[PMID]:26556631
[Au] Autor:Thakur K; Kaur M; Rabbani G; Khan RH; Singh S; Singh J
[Ti] Título:Structural variations and molten globule state in Arisaema helliborifolium lectin under various treatments as monitored by spectroscopy.
[So] Source:Protein Pept Lett;23(2):107-19, 2016.
[Is] ISSN:1875-5305
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Solvent perturbation was used to study variations in structure of Arisaema helliborifolium lectin (AHL) with the help of circular dichroism (CD), intrinsic fluorescence (IF), extrinsic fluorescence, quenching and dynamic light scattering (DLS). AHL was studied under acidic, alkaline and 6 M guanidine hydrochloride (GuHCl) equilibrium states. Three structural states were identified for AHL at different conditions, that are native (N; pH 7.0), molten globule (MG; pH 2.0) and unfolded (U; pH 12.0). CD analysis revealed that 50% of secondary structure of AHL was ß-sheet component. A complete loss of secondary structure was observed at GuHCl treatment. The tertiary structural changes as studied by changes in microenvironment of trp residues also suggested a pH induced MG state as in case of CD. Parameter-A analysis pointed at the multi-step unfolding process of lectin under varying pH (pH 1-13). A comparision of CD and IF data further indicated that different pathways were followed for secondary and tertiary structure unfolding. Tryptophans of native AHL were only partially exposed to solvent belonging to Class II. Hydrodynamic diameter (Dh ) measurements of AHL via DLS also confirmed of a pH induced molten globule. A thermally induced molten globule was identified for AHL between 54-60 °C as monitored by DLS. An irreversible thermal denaturation was observed with the formation of a large aggregate. The Dh of AHL at neutral pH was confirmed by transmission electron microscopy (TEM).
[Mh] Termos MeSH primário: Arisaema/química
Lectinas/química
Estrutura Secundária de Proteína
Termodinâmica
[Mh] Termos MeSH secundário: Dicroísmo Circular
Difusão Dinâmica da Luz
Fluorescência
Concentração de Íons de Hidrogênio
Desnaturação Proteica
Dobramento de Proteína
Triptofano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151112
[St] Status:MEDLINE


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[PMID]:25407163
[Au] Autor:Dong G; Wang J; Guo P; Wei D; Yang M; Kong L
[Ad] Endereço:State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, P.R. China. cpu_lykong@126.com.
[Ti] Título:Toxicity assessment of Arisaematis Rhizoma in rats by a (1)H NMR-based metabolomics approach.
[So] Source:Mol Biosyst;11(2):407-17, 2015 Feb.
[Is] ISSN:1742-2051
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arisaematis Rhizoma (AR), a famous traditional Chinese medicine, has been widely used in Asia over thousands of years. Documented with noticeable toxicity in ancient books, AR has been used to treat various diseases in the clinic. Therefore, it is important to assess the toxicity of AR dynamically and holistically. In this study, a (1)H NMR-based metabolomics approach complemented with serum chemistry and histopathology has been applied to investigate the toxicity of AR. Rats were intragastrically administered with AR (0, 0.5 and 1 g kg(-1) body weight) for 30 days, and serum and urine samples were collected. Their (1)H NMR profiles were analyzed by multivariate pattern recognition techniques to denote metabolic variations induced by AR, and 13 metabolites in urine and 6 metabolites in serum were significantly altered, which suggested that disturbances in energy metabolism, perturbation of the gut microflora environment, membrane damage, folate deficiency and injury of kidneys are produced by AR. Histopathology showed a slight vacuolization of the glomerular matrix and edema of renal tubular epithelial cells in kidneys of AR administered rats, which were evidenced by increased levels of blood urea nitrogen and creatinine in serum chemistry. Our results indicated that oral administration of crude AR was found to induce slight renal toxicity. Therefore, precautions should be made to monitor the potential nephrotoxicity of AR in clinical use. The metabolomics approach provided a promising tool for the study and better understanding of TCM-induced toxicity dynamically and holistically.
[Mh] Termos MeSH primário: Arisaema/química
Medicamentos de Ervas Chinesas/toxicidade
Metabolômica/métodos
Espectroscopia de Prótons por Ressonância Magnética
Rizoma/química
Testes de Toxicidade
[Mh] Termos MeSH secundário: Animais
Peso Corporal/efeitos dos fármacos
Análise Discriminante
Rim/efeitos dos fármacos
Rim/patologia
Análise dos Mínimos Quadrados
Masculino
Metaboloma/efeitos dos fármacos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150121
[Lr] Data última revisão:
150121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141120
[St] Status:MEDLINE
[do] DOI:10.1039/c4mb00583j


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[PMID]:25902639
[Au] Autor:Wang X; Chi Y; Kang A
[Ti] Título:[Qualitative and quantitative analysis of the amino acids in Rhizoma Arisaematis by ultra high performance liquid chromatography-tandem mass spectrometry and high performance liquid chromatography].
[So] Source:Se Pu;32(12):1326-32, 2014 Dec.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:A method for the identification and determination of the polar amino components without ultraviolet activity in traditional Chinese medicines was developed. With Rhizoma Arisaematis as the object of this study, using pre-column derivatization with phenyl isothiocyanate (PITC) as the derivatization reagent, compounds were separated and identified on a C18 column (100 mm x 2.1 mm, 3.5 µm) by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A total of 20 components, including 18 amino acids and 2 amine compounds were identified. Furthermore, after the optimization of the derivatization conditions, 15 amino acids were determined by high performance liquid chromatography (HPLC) on Diamonsil C18 column (250 mm x 4.6 mm, 5 µm), detected at 254 nm and gradiently eluted by acetonitrile and 0. 05 mol/L ammonium acetate-acetic acid (pH 6. 5) as the mobile phases. The results of methodological study demonstrated that the method can meet the requirements of the determination. All calibration curves expressed good linearity: Glu, Try in the range of 2-100 mg/L, Arg in the range of 6-300 mg/L, others in the range of 0. 8-40 µg/L, with the correlation coefficients ≥ 0. 999 5. The average recovery of this method was among 95%-105% and the RSD was less than 3%. The developed method was successfully applied to quantitative determination of amino compounds in 12 batches of Rhizoma Arisaematis samples. The method is simple, sensitive, accurate, and can be used for rapid identification and determination of amino components in traditional Chinese medicines.
[Mh] Termos MeSH primário: Aminoácidos/análise
Arisaema/química
Cromatografia Líquida de Alta Pressão
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Medicamentos de Ervas Chinesas
Medicina Tradicional Chinesa
Rizoma/química
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150424
[St] Status:MEDLINE


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[PMID]:25244739
[Au] Autor:Shi LC; Chen J; Xiang L; Song JY; Yao H
[Ti] Título:[DNA barcoding identification between arisaematis rhizoma and its adulterants based on ITS2 sequences].
[So] Source:Zhongguo Zhong Yao Za Zhi;39(12):2176-9, 2014 Jun.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified, sequenced and assembled for all of the collected samples. Then, ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore, three origin species, Arisaema amurense, A. erubescens and A. heterophyllum, can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective.
[Mh] Termos MeSH primário: Arisaema/classificação
Código de Barras de DNA Taxonômico/métodos
DNA de Plantas/genética
DNA Espaçador Ribossômico/genética
Medicamentos de Ervas Chinesas/classificação
[Mh] Termos MeSH secundário: Arisaema/genética
Contaminação de Medicamentos/prevenção & controle
Medicamentos de Ervas Chinesas/química
Dados de Sequência Molecular
Filogenia
Controle de Qualidade
Rizoma/classificação
Rizoma/genética
[Pt] Tipo de publicação:ENGLISH ABSTRACT; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer); 0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140923
[Lr] Data última revisão:
140923
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140924
[St] Status:MEDLINE


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[PMID]:24895548
[Au] Autor:Sudan R; Bhagat M; Gupta S; Singh J; Koul A
[Ad] Endereço:School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir 180006, India.
[Ti] Título:Iron (FeII) chelation, ferric reducing antioxidant power, and immune modulating potential of Arisaema jacquemontii (Himalayan Cobra Lily).
[So] Source:Biomed Res Int;2014:179865, 2014.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study explored the antioxidant and immunomodulatory potential of ethnomedicinally valuable species, namely, Arisaema jacquemontii of north-western Himalayan region. The tubers, leaves, and fruits of this plant were subjected to extraction using different solvents. In vitro antioxidant studies were performed in terms of chelation power on ferrous ions and FRAP assay. The crude methanol extract of leaves was found to harbour better chelating capacity (58% at 100 µ g/mL) and reducing power (FRAP value 1085.4 ± 0.11 µ MFe(3+)/g dry wt.) than all the other extracts. The crude methanol extract was thus further partitioned with solvents to yield five fractions. Antioxidant study of fractions suggested that the methanol fraction possessed significant chelation capacity (49.7% at 100 µ g/mL) and reducing power with FRAP value of 1435.4 µ M/g dry wt. The fractions were also studied for immune modulating potential where it was observed that hexane fraction had significant suppressive effect on mitogen induced T-cell and B-cell proliferation and remarkable stimulating effect on humoral response by 141% and on DTH response by 168% in immune suppressed mice as compared to the controls. Therefore, it can be concluded that A. jacquemontii leaves hold considerable antioxidant and immunomodulating potential and they can be explored further for the identification of their chemical composition for a better understanding of their biological activities.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Arisaema/química
Fatores Imunológicos/farmacologia
Quelantes de Ferro/farmacologia
Ferro/metabolismo
Substâncias Redutoras/farmacologia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Concanavalina A/farmacologia
Compostos Ferrosos/metabolismo
Imunidade Celular/efeitos dos fármacos
Imunidade Humoral/efeitos dos fármacos
Lipopolissacarídeos/farmacologia
Linfócitos/citologia
Linfócitos/efeitos dos fármacos
Masculino
Metanol/química
Camundongos
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/farmacologia
Folhas de Planta/química
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Ferrous Compounds); 0 (Immunologic Factors); 0 (Iron Chelating Agents); 0 (Lipopolysaccharides); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Reducing Agents); 11028-71-0 (Concanavalin A); E1UOL152H7 (Iron); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140605
[St] Status:MEDLINE
[do] DOI:10.1155/2014/179865


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[PMID]:24093755
[Au] Autor:Nile SH; Park SW
[Ad] Endereço:Department of Molecular Biotechnology, College of Life and Environmental Sciences, Konkuk University , Seoul , South Korea.
[Ti] Título:HPTLC analysis, antioxidant, anti-inflammatory and antiproliferative activities of Arisaema tortuosum tuber extract.
[So] Source:Pharm Biol;52(2):221-7, 2014 Feb.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Oxidative stress and inflammation are related to several chronic diseases including cancer and atherosclerosis. Arisaema tortuosum (Wall.) Schott (Araceae) is an Indian folk medicinal herb traditionally used for treatment of various diseases related to inflammation and stress. OBJECTIVE: This study was carried out for HPTLC analysis and evaluation of antioxidant, anti-inflammatory and antiproliferative activities of a methanol extract of A. tortuosum tuber. MATERIALS AND METHODS: The antioxidant activities of methanol extract of A. tortuosum tuber (1 mg/mL) were evaluated by DPPH, ABTS and FRAP assays and anti-inflammatory effects by diene-conjugate and ß-glucuronidase assays, with in vitro tumor growth inhibition on HeLa cancer cells. The results for antioxidant and anti-inflammatory effects were compared using Trolox and salicylic acid as reference compounds, respectively. RESULTS: The TLC and HPTLC analysis showed the presence of quercetin, rutin, luteolin and lectin (Rf values 0.97, 0.53, 0.59 and 1.58, respectively). The methanol fraction of tuber exhibit higher activity in each antioxidant system with a special attention for DPPH (IC50 = 852 µg/mL), ABTS (IC50 = 532 µg/mL), and FRAP (IC50 = 458 µg/mL), as compared with Trolox as standard, with a remarkable amount of phenolics (86.2 mg/100 g) and flavonoids (175.5 mg/100 g), along with potent anti-inflammatory activity indicated by diene-conjugate (86.20%) and ß-glucuronidase (92.92%) inhibition, as compared with salicylic acid as reference compound. The antiproliferative activity at 100 mg/mL was 88% inhibition with HeLa cells. The inhibition of HeLa cell proliferation was greatest (p < 0.001) with the 100 mg/mL A. tortuosum tuber extract treatments and least with the 25 mg/mL dose. DISCUSSION AND CONCLUSION: Our results suggested that A. tortuosum tuber might be used as a promising and potent antioxidant, anti-inflammatory, and antiproliferative agent and might be used for standardization of potential drug after successful isolation and characterization of bioactive compounds.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/farmacologia
Arisaema/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/administração & dosagem
Anti-Inflamatórios/isolamento & purificação
Antineoplásicos Fitogênicos/administração & dosagem
Antineoplásicos Fitogênicos/isolamento & purificação
Antioxidantes/administração & dosagem
Antioxidantes/isolamento & purificação
Proliferação Celular/efeitos dos fármacos
Cromanos/farmacologia
Cromatografia em Camada Delgada/métodos
Células HeLa
Seres Humanos
Concentração Inibidora 50
Dose Máxima Tolerável
Extratos Vegetais/administração & dosagem
Extratos Vegetais/análise
Ácido Salicílico/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Chromans); 0 (Plant Extracts); O414PZ4LPZ (Salicylic Acid); S18UL9710X (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131008
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2013.831110


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[PMID]:23643241
[Au] Autor:Ryoo SM; Sohn CH; Oh BJ; Kim WY; Lim KS; Lee CC
[Ad] Endereço:Department of Emergency Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
[Ti] Título:Oropharyngeal airway obstruction after the accidental ingestion of Arisaema amurense.
[So] Source:J Emerg Med;45(3):352-4, 2013 Sep.
[Is] ISSN:0736-4679
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Arisaema amurense is widely known in Korean folklore as a poisonous plant, and its lethal toxicity has long been recognized. The toxicity of Arisaema amurense is due to its content of calcium oxalate, which causes painful oropharyngeal edema, hypersalivation, aphonia, oral ulceration, esophageal erosion, and hypocalcemia. OBJECTIVE: We report a case of accidental poisoning after ingestion of the rhizome of Arisaema amurense, resulting in airway obstruction that required endotracheal intubation. CASE REPORT: A 60-year-old man developed oral pain and swelling after accidentally ingesting a rhizome from the Arisaema amurense plant as a medicinal herb. His symptoms worsened upon his arrival in the Emergency Department, and he was unable to speak due to oral swelling and hypersalivation. The patient underwent endotracheal intubation to protect his airway and was treated with antihistamines and corticosteroids. Three days after treatment, he had improved and was extubated. CONCLUSION: We describe an emergent treatment course for a patient with acute airway obstruction resulting from the ingestion of Arisaema amurense.
[Mh] Termos MeSH primário: Obstrução das Vias Respiratórias/etiologia
Arisaema/envenenamento
Intoxicação por Plantas/complicações
[Mh] Termos MeSH secundário: Corticosteroides/uso terapêutico
Obstrução das Vias Respiratórias/terapia
Edema/etiologia
Antagonistas dos Receptores Histamínicos/uso terapêutico
Seres Humanos
Intubação Intratraqueal
Masculino
Meia-Idade
Orofaringe
Dor/etiologia
Intoxicação por Plantas/terapia
Sialorreia/etiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenal Cortex Hormones); 0 (Histamine Antagonists)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:130902
[Lr] Data última revisão:
130902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130507
[St] Status:MEDLINE



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