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Pesquisa : B01.650.940.800.575.912.250.618.050.750.109 [Categoria DeCS]
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[PMID]:27469097
[Au] Autor:Zhang S; Chen T; Li W; Dong Q; Xiong Y
[Ad] Endereço:Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China.
[Ti] Título:Physicochemical properties and combustion behavior of duckweed during wet torrefaction.
[So] Source:Bioresour Technol;218:1157-62, 2016 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Wet torrefaction of duckweed was carried out in the temperature range of 130-250°C to evaluate the effects on physicochemical properties and combustion behavior. The physicochemical properties of duckweed samples were investigated by ultimate analysis, proximate analysis, FTIR, XRD and SEM techniques. It was found that wet torrefaction improved the fuel characteristics of duckweed samples resulting from the increase in fixed carbon content, HHVs and the decrease in nitrogen and sulfur content and atomic ratios of O/C and H/C. It can be seen from the results of FTIR, XRD and SEM analyses that the dehydration, decarboxylation, solid-solid conversion, and condensation polymerization reactions were underwent during wet torrefaction. In addition, the results of thermogravimetric analysis (TGA) in air indicated that wet torrefaction resulted in significant changes on combustion behavior and combustion kinetics parameters. Duckweed samples after wet torrefaction behaved more char-like and gave better combustion characteristics than raw sample.
[Mh] Termos MeSH primário: Arum/química
[Mh] Termos MeSH secundário: Biomassa
Cinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE


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[PMID]:26599199
[Au] Autor:Ben-Arye E; Samuels N; Goldstein LH; Mutafoglu K; Omran S; Schiff E; Charalambous H; Dweikat T; Ghrayeb I; Bar-Sela G; Turker I; Hassan A; Hassan E; Saad B; Nimri O; Kebudi R; Silbermann M
[Ad] Endereço:Integrative Oncology Program, The Oncology Service and Lin Medical Center, Clalit Health Services, Haifa and Western Galilee District, Israel.
[Ti] Título:Potential risks associated with traditional herbal medicine use in cancer care: A study of Middle Eastern oncology health care professionals.
[So] Source:Cancer;122(4):598-610, 2016 Feb 15.
[Is] ISSN:1097-0142
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The authors assessed the use of herbal medicine by Middle Eastern patients with cancer, as reported by their oncology health care professionals (HCPs). Herbal products identified by the study HCPs were evaluated for potential negative effects. METHODS: Oncology HCPs from 16 Middle Eastern countries received a 17-item questionnaire asking them to list 5 herbal products in use by their patients with cancer. A literature search (PubMed, Micromedex, AltMedDex, and the Natural Medicine Comprehensive Database) was conducted to identify safety-related concerns associated with the products listed. RESULTS: A total of 339 HCPs completed the study questionnaire (response rate of 80.3%), identifying 44 herbal and 3 nonherbal nutritional supplements. Safety-related concerns were associated with 29 products, including herb-drug interactions with altered pharmacodynamics (15 herbs), direct toxic effects (18 herbs), and increased in vitro response of cancer cells to chemotherapy (7 herbs). CONCLUSIONS: Herbal medicine use, which is prevalent in Middle Eastern countries, has several potentially negative effects that include direct toxic effects, negative interactions with anticancer drugs, and increased chemosensitivity of cancer cells, requiring a reduction in dose-density. Oncology HCPs working in countries in which herbal medicine use is prevalent need to better understand the implications of this practice. The presence of integrative physicians with training in complementary and traditional medicine can help patients and their HCPs reach an informed decision regarding the safety and effective use of these products.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Interações Ervas-Drogas
Oncologia
Neoplasias/tratamento farmacológico
Fitoterapia/utilização
Preparações de Plantas/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Pessoal Técnico de Saúde
Animais
Arum
Camelus
Curcuma
Daucus carota
Feminino
Alho
Medicina Herbária/estatística & dados numéricos
Mel
Seres Humanos
Masculino
Meia-Idade
Oriente Médio
Leite
Nigella sativa
Médicos
Preparações de Plantas/uso terapêutico
Pesquisadores
Inquéritos e Questionários
Urtica dioica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Preparations)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE
[do] DOI:10.1002/cncr.29796


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[PMID]:26552380
[Au] Autor:Chartier M; Liagre S; Weiss-Schneeweiss H; Kolano B; Bessière JM; Schönenberger J; Gibernau M
[Ad] Endereço:Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030, Vienna, Austria. chartier.marion@gmail.com.
[Ti] Título:Floral traits and pollination ecology of European Arum hybrids.
[So] Source:Oecologia;180(2):439-51, 2016 Feb.
[Is] ISSN:1432-1939
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Hybridisation is common in plants and can affect the genetic diversity and ecology of sympatric parental populations. Hybrids may resemble the parental species in their ecology, leading to competition and/or gene introgression; alternatively, they may diverge from the parental phenotypes, possibly leading to the colonisation of new ecological niches and to speciation. Here, we describe inflorescence morphology, ploidy levels, pollinator attractive scents, and pollinator guilds of natural hybrids of Arum italicum and A. maculatum (Araceae) from a site with sympatric parental populations in southern France to determine how these traits affect the hybrid pollination ecology. Hybrids were characterised by inflorescences with a size and a number of flowers more similar to A. italicum than to A. maculatum. In most cases, hybrid stamens were purple, as in A. maculatum, and spadix appendices yellow, as in A. italicum. Hybrid floral scent was closer to that of A. italicum, but shared some compounds with A. maculatum and comprised unique compounds. Also, the pollinator guild of the hybrids was similar to that of A. italicum. Nevertheless, the hybrids attracted a high proportion of individuals of the main pollinator of A. maculatum. We discuss the effects of hybridisation in sympatric parental zones in which hybrids exhibit low levels of reproductive success, the establishment of reproductive barriers between parental species, the role of the composition of floral attractive scents in the differential attraction of pollinators and in the competition between hybrids and their parental species, and the potential of hybridisation to give rise to new independent lineages.
[Mh] Termos MeSH primário: Arum/genética
Flores
Hibridização Genética
Fenótipo
Polinização
[Mh] Termos MeSH secundário: Animais
Ecologia
Flores/anatomia & histologia
França
Inflorescência
Insetos
Odorantes
Feromônios
Ploidias
Reprodução
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151111
[St] Status:MEDLINE
[do] DOI:10.1007/s00442-015-3498-9


  4 / 37 MEDLINE  
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[PMID]:26284613
[Au] Autor:Afifi FU; Kasabri V; Litescu SC; Abaza IM
[Ad] Endereço:a Faculty of Pharmacy , The University of Jordan , Amman , Jordan.
[Ti] Título:In vitro and in vivo comparison of the biological activities of two traditionally and widely used Arum species from Jordan: Arum dioscoridis Sibth & Sm. and Arum palaestinum Boiss.
[So] Source:Nat Prod Res;30(16):1777-86, 2016 Aug.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arum dioscoridis and A. palaestinum (Araceae) are indigenous plant species in Jordan. HPLC-MS analysis of A. dioscoridis revealed the presence of apigenin, luteolin, quercetin, quercetin-3-O-ß-glucoside, vitexin, isoorientin, esculin, and caffeic and ferulic acids. Both Arum spp., influenced gastrointestinal carbohydrate and lipid digestion and absorption. Orlistat inhibited dose dependently and highly substantially pancreatic lipase (PL) in vitro. Similar to orlistat, Arum species aqueous extracts (AEs), apigenin, caffeic acid and esculin exhibited a concentration related PL inhibition. Comparable to acarbose, dual inhibition of α-amylase/α-glucosidase was observed for both Arum species. Like guar gum, A. dioscoridis AE minimised substantially area under 24 h glucose curve. Acute starch-induced postprandial hyperglycaemia in overnight fasting rats was highly significantly (p < 0.001) decreased by A. dioscoridis AE. A. palaestinum could not perform effectively in either starch- or glucose-fed fasting rats. No antiproliferative effects against colorectal cancer cell lines HT29, HCT116 and SW620 were detected for tested Arum spp.
[Mh] Termos MeSH primário: Arum/química
[Mh] Termos MeSH secundário: Acarbose/farmacologia
Animais
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Neoplasias Colorretais
Inibidores de Glicosídeo Hidrolases/farmacologia
Células HT29
Seres Humanos
Hiperglicemia/tratamento farmacológico
Jordânia
Extratos Vegetais/farmacologia
Ratos
alfa-Amilases/antagonistas & inibidores
alfa-Glucosidases
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoside Hydrolase Inhibitors); 0 (Plant Extracts); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.20 (alpha-Glucosidases); T58MSI464G (Acarbose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150819
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1072713


  5 / 37 MEDLINE  
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[PMID]:26243305
[Au] Autor:Cole C; Burgoyne T; Lee A; Stehno-Bittel L; Zaid G
[Ad] Endereço:Likarda, LLC, 2002 W. 39th Ave, Kansas City, KS, 66103, USA. ccole@likarda.com.
[Ti] Título:Arum Palaestinum with isovanillin, linolenic acid and ß-sitosterol inhibits prostate cancer spheroids and reduces the growth rate of prostate tumors in mice.
[So] Source:BMC Complement Altern Med;15:264, 2015 Aug 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Arum palaestinum is a plant commonly found in the Middle East that is ingested as an herbal remedy to fight cancer. However, no studies have examined the direct effect of the plant/plant extract on tumor growth in an animal model. METHODS: Verified prostate cancer cells were plated as 3D spheroids to determine the effect of extract from boiled Arum Palaestinum Boiss roots. In addition, male NU/NU mice (8 weeks old) with xenograft tumors derived from the prostate cancer cell line were treated daily with 1000 mg/kg body weight gavage of the suspension GZ17. The tumor growth was measured repeatedly with calipers and the excised tumors were weighed at the termination of the 3 week study. Control mice (10 mice in each group) received vehicle in the same manner and volume. RESULTS: The number of live prostate cancer cells declined in a dose/dependent manner with a 24 h exposure to the extract at doses of 0.015 to 6.25 mg/mL. A fortified version of the extract (referred to as GZ17) that contained higher levels of isovanillin, linolenic acid and ß-sitosterol had a stronger effect on the cell death rate, shifting the percentage of dead cells from 30 % to 55 % at the highest dose while the vehicle control had no effect on cell numbers. When GZ17 was applied to non-cancer tissue, in this case, human islets, there was no cell death at doses that were toxic to treated cancer cells. Preliminary toxicity studies were conducted on rats using an up-down design, with no signs of toxic effect at the highest dose. NU/NU mice with xenograft prostate tumors treated with GZ17 had a dramatic inhibition of tumor progression, while tumors in the control group grew steadily through the 3 weeks. The rate of tumor volume increase was 73 mm(3)/day for the vehicle group and 24 mm(3)/day for the GZ17 treated mice. While there was a trend towards lower excised tumor weight at study termination in the GZ17 treatment group, there was no statistical difference. CONCLUSIONS: Fortified Arum palaestinum Boiss caused a reduction in live cells within prostate cancer spheroids and blocked tumor growth in xenografted prostate tumors in mice without signs of toxicity.
[Mh] Termos MeSH primário: Antineoplásicos
Arum/química
Benzaldeídos
Extratos Vegetais
Neoplasias da Próstata
Sitosteroides
Ácido alfa-Linolênico
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/farmacologia
Antineoplásicos/uso terapêutico
Benzaldeídos/química
Benzaldeídos/farmacologia
Benzaldeídos/uso terapêutico
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Masculino
Camundongos
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Neoplasias da Próstata/tratamento farmacológico
Neoplasias da Próstata/patologia
Ratos
Sitosteroides/química
Sitosteroides/farmacologia
Sitosteroides/uso terapêutico
Esferoides Celulares
Células Tumorais Cultivadas
Ensaios Antitumorais Modelo de Xenoenxerto
Ácido alfa-Linolênico/química
Ácido alfa-Linolênico/farmacologia
Ácido alfa-Linolênico/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Benzaldehydes); 0 (Plant Extracts); 0 (Sitosterols); 0RBV727H71 (alpha-Linolenic Acid); 4A9N90H9X6 (isovanillin); 5LI01C78DD (gamma-sitosterol)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150806
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-015-0774-5


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[PMID]:25736477
[Au] Autor:Onda Y; Mochida K; Yoshida T; Sakurai T; Seymour RS; Umekawa Y; Pirintsos SA; Shinozaki K; Ito K
[Ad] Endereço:1] Biomass Research Platform Team, Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan [2] Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan.
[Ti] Título:Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
[So] Source:Sci Rep;5:8753, 2015 Mar 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-D-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.
[Mh] Termos MeSH primário: Arum/genética
Flores/genética
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica de Plantas
[Mh] Termos MeSH secundário: Arum/metabolismo
Vias Biossintéticas/genética
Metabolismo Energético/genética
Flores/química
Flores/metabolismo
Ontologia Genética
Proteínas Mitocondriais/genética
Proteínas Mitocondriais/metabolismo
Odorantes/análise
Oxirredutases/genética
Oxirredutases/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Temperatura Ambiente
Terpenos/metabolismo
Transferases/genética
Transferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Mitochondrial Proteins); 0 (Plant Proteins); 0 (Terpenes); EC 1.- (Oxidoreductases); EC 1.- (alternative oxidase); EC 2.- (Transferases); EC 2.2.1.- (deoxyxylulose-5-phosphate synthase)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170416
[Lr] Data última revisão:
170416
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150305
[St] Status:MEDLINE
[do] DOI:10.1038/srep08753


  7 / 37 MEDLINE  
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[PMID]:25520041
[Au] Autor:Karahan F; Kulak M; Urlu E; Gözüacik HG; Böyümez T; Sekeroglu N; Doganturk IH
[Ad] Endereço:a Department of Biology , Faculty of Arts and Sciences, Kilis 7 Aralik University , 79000 Kilis , Turkey.
[Ti] Título:Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis.
[So] Source:Nat Prod Res;29(17):1678-83, 2015.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arum dioscoridis, locally called 'Gavur pancari', is a wild plant the leaves of which have been used as vegetable and for preparing special soup which has a sour taste. This study was set up to determine in vitro antioxidant activities and total phenolic and flavonoid contents of different extracts of A. dioscoridis. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing activity with different concentrations of ethanol, methanol, acetone and water extracts of the plant leaves. Total phenolic and flavonoid contents were widely variable depending on solvents. Ethanol and methanol extractions of the plant material showed better performances with respect to both phenolic and flavonoid contents, respectively. The highest phenolic and flavonoid contents of ethanol and methanol extracts were 100.890 mg/g GAE and 72.643 mg/g QE, respectively. The lower DPPH scavenging and ferric reducing activities were determined in comparison with previous reports and standard synthetic chemicals.
[Mh] Termos MeSH primário: Arum/química
Depuradores de Radicais Livres/química
Extratos Vegetais/química
Polifenóis/química
[Mh] Termos MeSH secundário: Compostos de Bifenilo
Flavonoides/química
Oxirredução
Picratos
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Flavonoids); 0 (Free Radical Scavengers); 0 (Picrates); 0 (Plant Extracts); 0 (Polyphenols); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141219
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2014.991320


  8 / 37 MEDLINE  
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[PMID]:25256124
[Au] Autor:Seymour RS; Ito K; Umekawa Y; Matthews PD; Pirintsos SA
[Ad] Endereço:School of Biological Sciences, University of Adelaide, Adelaide, South Australia, 5005, Australia.
[Ti] Título:The oxygen supply to thermogenic flowers.
[So] Source:Plant Cell Environ;38(4):827-37, 2015 Apr.
[Is] ISSN:1365-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thermogenic flowers produce heat by intense respiration, and the rates of O2 consumption (Mo2) in some species can exceed those of all other tissues of plants and most animals. By exposing intact flowers to a range of O2 pressures (Po2) and measuring Mo2, we demonstrate that the highest respiration rates exceed the capacity of the O2 diffusive pathway and become diffusion limited in atmospheric air. The male florets on the inflorescence of Arum concinnatum have the highest known mass-specific Mo2 and can be severely diffusion limited. Intact spadices of Japanese skunk cabbage Symplocarpus renifolius are diffusion limited in air only when Mo2 is maximal, but not at lower levels. True flowers of the sacred lotus Nelumbo nucifera and the appendix of Arum concinnatum are never diffusion limited in air. Mo2 - Po2 curves are evaluated quantitatively with the 'Regulation Index', a new tool to measure dependence of Mo2 on ambient Po2 , as well as the conventional 'Critical Po2 '. The study also includes measurements of Po2 within thermogenic tissues with O2-sensitive fibre optics, and reveals that the diffusion pathway is complicated and that O2 can be provided not only from the surface of the tissues but also from the pith of the flower's peduncle.
[Mh] Termos MeSH primário: Araceae/fisiologia
Arum/fisiologia
Flores/fisiologia
Nelumbo/fisiologia
Oxigênio/metabolismo
Transpiração Vegetal/fisiologia
[Mh] Termos MeSH secundário: Respiração Celular
Difusão
Temperatura Alta
Inflorescência/fisiologia
Oxigênio/análise
Análise de Regressão
Termogênese/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
S88TT14065 (Oxygen)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150316
[Lr] Data última revisão:
150316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140927
[St] Status:MEDLINE
[do] DOI:10.1111/pce.12454


  9 / 37 MEDLINE  
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[PMID]:24925357
[Au] Autor:Leguet A; Gibernau M; Shintu L; Caldarelli S; Moja S; Baudino S; Caissard JC
[Ad] Endereço:Laboratoire BVpam, EA 3061, Université Jean Monnet, Université de Lyon, Saint-Etienne, France.
[Ti] Título:Evidence for early intracellular accumulation of volatile compounds during spadix development in Arum italicum L. and preliminary data on some tropical Aroids.
[So] Source:Naturwissenschaften;101(8):623-35, 2014 Aug.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Staining and histochemistry of volatile organic compounds (VOCs) were performed at different inflorescence developmental stages on nine aroid species; one temperate, Arum italicum and eight tropical from the genera Caladium, Dieffenbachia and Philodendron. Moreover, a qualitative and quantitative analysis of VOCs constituting the scent of A. italicum, depending on the stage of development of inflorescences was also conducted. In all nine species, vesicles were observed in the conical cells of either the appendix or the stamens (thecae) and the staminodes. VOCs were localised in intracellular vesicles from the early stages of inflorescence development until their release during receptivity of gynoecium. This localisation was observed by the increase of both number and diameter of the vesicles during 1 week before receptivity. Afterwards, vesicles were fewer and smaller but rarely absent. In A. italicum, staining and gas chromatography analyses confirmed that the vesicles contained terpenes. The quantitatively most important ones were the sesquiterpenes, but monoterpenes were not negligible. Indeed, the quantities of terpenes matched the vesicles' size evolution during 1 week. Furthermore, VOCs from different biosynthetic pathways (sesquiterpenes and alkanes) were at their maximum quantity 2 days before gynoecium receptivity (sesquiterpenes and alkanes) or during receptivity (isobutylamine, monoterpenes, skatole and p-cresol). VOCs seemed to be emitted during gynoecium receptivity and/or during thermogenesis, and FADs are accumulated after thermogenesis in the spadix. These complex dynamics of the different VOCs could indicate specialisation of some VOCs and cell machinery to attract pollinators on the one hand and to repulse/protect against phytophagous organisms and pathogens after pollination on the other hand.
[Mh] Termos MeSH primário: Araceae/química
Arum/química
Polinização
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Araceae/crescimento & desenvolvimento
Arum/crescimento & desenvolvimento
Cromatografia Gasosa
Folhas de Planta/química
Terpenos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Terpenes); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140614
[St] Status:MEDLINE
[do] DOI:10.1007/s00114-014-1197-8


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[PMID]:23653140
[Au] Autor:Kakizaki Y; Ito K
[Ad] Endereço:The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. kakizakiyusuke@gmail.com
[Ti] Título:Engineering plant alternative oxidase function in mammalian cells: substitution of the motif-like sequence ENV for QDT diminishes catalytic activity of Arum concinnatum AOX1a expressed in HeLa cells.
[So] Source:Appl Biochem Biotechnol;170(5):1229-40, 2013 Jul.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alternative oxidase (AOX) is a nonproton motive quinol-oxygen oxidoreductase which is a component of the mitochondrial respiratory chain in higher plants. In this study, we have characterized the catalytic activity and regulatory behaviors of Arum concinnatum AOX isoforms, namely AcoAOX1a and AcoAOX1b, and their artificial mutants in HeLa cells. We demonstrated that substitution of the motif-like sequence ENV on the C-terminal half of AcoAOX1a for QDT diminishes its activity and proposed that the innate inactivity of AcoAOX1b in HeLa cells is, at least in part, attributable to its QDT motif. Furthermore, we show that introduction of F130L in the hydrophilic N-terminal extension of AcoAOX1a resulted in greater activity in the presence of pyruvate. This result indicates that functional significance of the N-terminal extension is not particular to the conventional regulatory cysteine. On the basis of these findings, we discuss new insights into the structural integrity of AOX in HeLa cells and the applicability of mammalian cells for functional analysis of this enzyme.
[Mh] Termos MeSH primário: Arum/química
Arum/fisiologia
Proteínas Mitocondriais/química
Proteínas Mitocondriais/metabolismo
Oxirredutases/química
Oxirredutases/metabolismo
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Engenharia de Proteínas/métodos
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Substituição de Aminoácidos
Catálise
Ativação Enzimática
Células HeLa
Seres Humanos
Proteínas Mitocondriais/genética
Oxirredutases/genética
Proteínas de Plantas/genética
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Mitochondrial Proteins); 0 (Plant Proteins); EC 1.- (Oxidoreductases); EC 1.- (alternative oxidase)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:130701
[Lr] Data última revisão:
130701
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130509
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-013-0235-x



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