Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.100.050.060.800 [Categoria DeCS]
Referências encontradas : 36 [refinar]
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[PMID]:27749248
[Au] Autor:Poblocka-Olech L; Glód D; Zebrowska ME; Sznitowska M; Krauze-Baranowska M
[Ti] Título:TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum.
[So] Source:Acta Pharm;66(4):543-554, 2016 Dec 01.
[Is] ISSN:1846-9558
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4'-O-glucoside and quercetin-3,4'-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 ('Exihibition') to 1079 mg kg-1 fresh mass (fm) ('Hybing') in edible parts, and from 1727 ('Hyline') to 28949 mg kg-1 fm ('Red Baron') in outer scales. The bulbs of two shallot cultivars contained 209 ('Ambition') and 523 mg kg-1 fm ('Matador') of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively.
[Mh] Termos MeSH primário: Antioxidantes/análise
Química Farmacêutica/métodos
Produtos Agrícolas/química
Flavonoides/análise
Cebolas/química
Raízes de Plantas/química
Cebolinha Branca/química
[Mh] Termos MeSH secundário: Antioxidantes/isolamento & purificação
Calibragem
Cromatografia de Fase Reversa
Cromatografia em Camada Delgada
Densitometria
Flavonoides/isolamento & purificação
Glucosídeos/análise
Glucosídeos/isolamento & purificação
Limite de Detecção
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Polônia
Controle de Qualidade
Quercetina/análogos & derivados
Quercetina/análise
Quercetina/isolamento & purificação
Reprodutibilidade dos Testes
Especificidade da Espécie
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids); 0 (Glucosides); 0 (Plant Extracts); 0 (quercetin-3,4'-O-diglucoside); 9IKM0I5T1E (Quercetin); K2B74751XI (spiraeoside)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27187504
[Au] Autor:Kim SW; Rahman MM; Abd El-Aty AM; Truong LT; Choi JH; Park JS; Kim MR; Shin HC; Shim JH
[Ad] Endereço:Biotechnology Research Institute, College of Agriculture and Life Science, Chonnam National University, Yongbong-ro 77, Buk-gu, Gwangju, 500-757, Republic of Korea.
[Ti] Título:Residue level and dissipation pattern of lepimectin in shallots using high-performance liquid chromatography coupled with photodiode array detection.
[So] Source:Biomed Chromatogr;30(11):1835-1842, 2016 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lepimectin, as an emulsifiable concentrate, was sprayed on shallots at the recommended dose rate (10 mL/20 L) to determine its residue levels, dissipation pattern, pre-harvest residue limits (PHRLs), and health risk. Samples were randomly collected over 10 days, extracted with acetonitrile, purified using an amino solid-phase extraction (NH -SPE) cartridge and analyzed using a high-performance liquid chromatography-photodiode array detection method. Field-incurred samples were confirmed using ultra-performance liquid chromatography-tandem mass spectrometry. The linearity was excellent, with a determination coefficient (R ) of ≥0.9991. The recoveries at two spiking levels (0.2 and 1.0 mg/kg) ranged from 84.49 to 87.64% with relative standard deviations of ≤7.04%. The developed method was applied to field samples grown in separate greenhouses, one located in Naju and one in Muan, in the Republic of Korea. The dissipation pattern was described by first-order kinetics with half-lives of 1.9 (Naju) and 1.7 days (Muan). The PHRL curves indicated that, if the lepimectin residues are <0.18 (Naju) and <0.13 mg/kg (Muan) 5 days before harvest, the residue levels will be lower than the maximum residue limit (0.05 mg/kg) upon harvesting. The risk assessment data indicated that lepimectin is safe for use in the cultivation of shallots, with no risk of detrimental effects to the consumer.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Aditivos Alimentares/análise
Lactonas/análise
Compostos Macrocíclicos/análise
Resíduos de Praguicidas/análise
Cebolinha Branca/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Análise de Alimentos/métodos
Limite de Detecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Food Additives); 0 (Lactones); 0 (Macrocyclic Compounds); 0 (Pesticide Residues); 0 (lepimectin A3); 0 (lepimectin A4)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3759


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[PMID]:26218854
[Au] Autor:Wako T; Yamashita K; Tsukazaki H; Ohara T; Kojima A; Yaguchi S; Shimazaki S; Midorikawa N; Sakai T; Yamauchi N; Shigyo M
[Ad] Endereço:a NARO Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, 360 Ano-Kusawa, Tsu, Mie 514-2392, Japan.
[Ti] Título:Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition.
[So] Source:Genome;58(4):135-42, 2015 Apr.
[Is] ISSN:1480-3321
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum - shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.
[Mh] Termos MeSH primário: Basidiomycota/fisiologia
Cromossomos de Plantas/genética
Resistência à Doença/genética
Cebolas/genética
Doenças das Plantas/imunologia
Cebolinha Branca/genética
[Mh] Termos MeSH secundário: Basidiomycota/imunologia
Cruzamento
Cebolas/imunologia
Cebolas/microbiologia
Doenças das Plantas/microbiologia
Folhas de Planta/genética
Folhas de Planta/imunologia
Folhas de Planta/microbiologia
Plântulas/genética
Plântulas/imunologia
Plântulas/microbiologia
Cebolinha Branca/imunologia
Cebolinha Branca/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150729
[St] Status:MEDLINE
[do] DOI:10.1139/gen-2015-0026


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[PMID]:26211100
[Au] Autor:Saenthaweesuk S; Jitvaropas R; Somparn N; Thuppia A
[Ti] Título:An investigation of antimicrobial and wound healing potential of Allium ascalonicum Linn.
[So] Source:J Med Assoc Thai;98 Suppl 2:S22-7, 2015 Mar.
[Is] ISSN:0125-2208
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The pharmacological properties of Allium ascalonicum Linn., commonly called shallot, have been reported as including those that are antibacterial and antioxidant. OBJECTIVE: The present study aimed to evaluate the antimicrobial effect and wound-healing activity ofthe ethanolic extracts of Allium ascalonicum Linn. (AAE). MATERIAL AND METHOD: The antimicrobial activity of AAE was tested in vitro against using the disc diffusion method and a broth micro-dilution technique to determine the minimal inhibition concentrations (MIC) and the minimal microbicidal concentrations (MMC). Wound-healing activity of the extract was performed on rat test subjects. RESULTS: The AAE showed potential antimicrobial activity by inhibiting gram-positive bacteria Staphylococcus epidermidis and Bacillus subtilis ATCC 6633. MIC and MMC varied from 25-50 mg/ml and 25-200 mg/ml, respectively. After surgery 14 days, wound contractions oftreated groups and standard group were 78.61 +/- 1.20%, 78.55 +/- 1.93% and 100%, respectively; but, in the control group, wound contraction was 64.90 +/- 3.55%. Histological studies showed the complete epidermis and found the collagen fibers and fibroblasts as similar appearance as standard group in dermis. The results of histological evaluation have confirmed remarkable wound-healing activities of AAE. CONCLUSION: Taken together the present study provides evidence that AAE extract processes antimicrobial and wound-healing activities.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Extratos Vegetais/farmacologia
Cebolinha Branca/química
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bactérias/efeitos dos fármacos
Masculino
Testes de Sensibilidade Microbiana
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150727
[Lr] Data última revisão:
150727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150728
[St] Status:MEDLINE


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[PMID]:25652946
[Au] Autor:Kongstad KT; Özdemir C; Barzak A; Wubshet SG; Staerk D
[Ad] Endereço:Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, DK-2100 Copenhagen, Denmark.
[Ti] Título:Combined use of high-resolution α-glucosidase inhibition profiling and high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for investigation of antidiabetic principles in crude plant extracts.
[So] Source:J Agric Food Chem;63(8):2257-63, 2015 Mar 04.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Type 2 diabetes is a metabolic disorder affecting millions of people worldwide, and new drug leads or functional foods containing selective α-glucosidase inhibitors are needed. Crude extract of 24 plants were assessed for α-glucosidase inhibitory activity. Methanol extracts of Cinnamomum zeylanicum bark, Rheum rhabarbarum peel, and Rheum palmatum root and ethyl acetate extracts of C. zeylanicum bark, Allium ascalonicum peel, and R. palmatum root showed IC50 values below 20 µg/mL. Subsequently, high-resolution α-glucosidase profiling was used in combination with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for identification of metabolites responsible for the α-glucosidase inhibitory activity. Quercetin (1) and its dimer (2), trimer (3), and tetramer (4) were identified as main α-glucosidase inhibitors in A. ascalonicum peel, whereas (E)-piceatannol 3'-O-ß-D-glucopyranoside (5), (E)-rhapontigenin 3'-O-ß-D-glucopyranoside (6), (E)-piceatannol (8), and emodin (12) were identified as main α-glucosidase inhibitors in R. palmatum root.
[Mh] Termos MeSH primário: Cinnamomum/química
Hipoglicemiantes/química
Hipoglicemiantes/isolamento & purificação
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Rheum/química
Cebolinha Branca/química
[Mh] Termos MeSH secundário: Diabetes Mellitus Tipo 2/enzimologia
Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Seres Humanos
Espectroscopia de Ressonância Magnética/métodos
Espectrometria de Massas/métodos
Extração em Fase Sólida/métodos
alfa-Glucosidases/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycoside Hydrolase Inhibitors); 0 (Hypoglycemic Agents); 0 (Plant Extracts); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150304
[Lr] Data última revisão:
150304
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150206
[St] Status:MEDLINE
[do] DOI:10.1021/jf506297k


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[PMID]:24840922
[Au] Autor:Tocmo R; Lin Y; Huang D
[Ad] Endereço:Food Science and Technology Programme, Department of Chemistry, National University of Singapore , 117543 Singapore.
[Ti] Título:Effect of processing conditions on the organosulfides of shallot (Allium cepa L. Aggregatum group).
[So] Source:J Agric Food Chem;62(23):5296-304, 2014 Jun 11.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is a growing account of the health benefits of H2S as an endogenous cell-signaling molecule. H2S from organic polysulfides, in particular, is increasingly gaining attention for their beneficial effects to cardiovascular health. Here, we studied shallot as a potential dietary source of organic polysulfides and examined the effects of processing conditions on its polysulfide profiles. Boiling, autoclaving, and freeze-drying were tested on whole and crushed shallot bulbs, analyzing their effect on the yield of organosulfides. Seventeen organosulfides, including disulfides, trisulfides, and cyclic polysulfides, were identified. Significant differences in the quantitative and qualitative profiles of organosulfides in the hydrodistilled and solvent extracted oils were observed. Freeze-drying retained the majority of the organosulfides, but the whole-autoclaved and whole-boiled shallots lost more than 95% of their organic polysulfides. Crushed-boiled and crushed-autoclaved shallot lost 76-80% of their organosulfides, likely due to the thermal sensitivity of these compounds. The organosulfide profiles are sensitive to the pH values of the processing media. In general, disulfides increased at basic pH (pH 9.0) while trisulfides and cyclic organosulfides are much higher at the acidic to neutral pH values (pH 3.0-5.0). Our results provide important information on the effects of processing conditions that are relevant for optimizing extraction of organosulfides from shallot for further studies evaluating their H2S-releasing activity.
[Mh] Termos MeSH primário: Manipulação de Alimentos/métodos
Extratos Vegetais/química
Cebolinha Branca/química
Sulfetos/química
[Mh] Termos MeSH secundário: Culinária
Raízes de Plantas/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Sulfides)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140611
[Lr] Data última revisão:
140611
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140521
[St] Status:MEDLINE
[do] DOI:10.1021/jf500739n


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[PMID]:24721027
[Au] Autor:Krejcová P; Kucerová P; Stafford GI; Jäger AK; Kubec R
[Ad] Endereço:Department of Applied Chemistry, University of South Bohemia, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
[Ti] Título:Antiinflammatory and neurological activity of pyrithione and related sulfur-containing pyridine N-oxides from Persian shallot (Allium stipitatum).
[So] Source:J Ethnopharmacol;154(1):176-82, 2014 May 28.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Persian shallot (Allium stipitatum) is a bulbous plant native to Turkey, Iran and Central Asia. It is frequently used in folk medicine for the treatment of a variety of disorders, including inflammation and stress. Antiinflammatory and neurological activities of pyrithione and four related sulfur-containing pyridine N-oxides which are prominent constituents of Allium stipitatum were tested. METHODS: The antiinflammatory activity was tested by the ability of the compounds to inhibit cyclooxygenase (COX-1 and COX-2), whereas the neurological activities were evaluated by assessing the compounds ability to inhibit monoamine oxidase-A (MAO-A) and acetylcholinesterase (AChE). The compounds׳ affinity for the serotonin transport protein (SERT) and the GABAA-benzodiazepine receptor were also investigated. RESULTS: 2-[(Methylthio)methyldithio]pyridine N-oxide showed very high antiinflammatory effects which are comparable with those of common pharmaceuticals (IC50 of 7.8 and 15.4 µM for COX-1 and COX-2, respectively). On the other hand, neurological activities of the compounds were rather modest. Some compounds moderately inhibited AChE (IC50 of 104-1041 µM) and MAO-A (IC50 of 98-241 µM) and exhibited an affinity for the SERT and GABAA-benzodiazepine receptor. CONCLUSIONS: Our findings may help to rationalize the wide use of Persian shallot for the treatment of inflammatory disorders.
[Mh] Termos MeSH primário: Inibidores da Colinesterase/farmacologia
Inibidores de Ciclo-Oxigenase/farmacologia
Inibidores da Monoaminoxidase/farmacologia
Piridinas/farmacologia
Cebolinha Branca
Tionas/farmacologia
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Animais
Encéfalo/efeitos dos fármacos
Encéfalo/metabolismo
Ciclo-Oxigenase 1/metabolismo
Ciclo-Oxigenase 2/metabolismo
Peroxidase do Rábano Silvestre
Monoaminoxidase/metabolismo
Proteínas de Ligação a RNA/metabolismo
Ratos
Receptores de GABA-A/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Cyclooxygenase Inhibitors); 0 (Monoamine Oxidase Inhibitors); 0 (Pyridines); 0 (RNA-Binding Proteins); 0 (Receptors, GABA-A); 0 (SERT protein, rat); 0 (Thiones); 6GK82EC25D (pyrithione); 91F12JJJ4H (pyridine N-oxide); EC 1.11.1.- (Horseradish Peroxidase); EC 1.14.99.1 (Cyclooxygenase 1); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); EC 1.4.3.4 (Monoamine Oxidase); EC 1.4.3.4. (monoamine oxidase A, human); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140513
[Lr] Data última revisão:
140513
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140412
[St] Status:MEDLINE


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[PMID]:24150581
[Au] Autor:Arkhipov AV; Gushchin VA; Vishnichenko VK; Solovyev AG
[Ad] Endereço:Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, Moscow, 127550, Russia.
[Ti] Título:Accumulation of changes in the genome of shallot virus X persisting in vegetatively reproduced plants.
[So] Source:Dokl Biochem Biophys;452(1):237-40, 2013 Sep.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Mh] Termos MeSH primário: Flexiviridae/genética
Genoma Viral
Polimorfismo Genético
Cebolinha Branca/virologia
[Mh] Termos MeSH secundário: DNA Viral
Reprodução Assexuada
Cebolinha Branca/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131024
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672913050049


  9 / 36 MEDLINE  
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[PMID]:24138065
[Au] Autor:Teshima Y; Ikeda T; Imada K; Sasaki K; El-Sayed MA; Shigyo M; Tanaka S; Ito S
[Ad] Endereço:Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Yamaguchi 753-8515, Japan.
[Ti] Título:Identification and biological activity of antifungal saponins from shallot ( Allium cepa L. Aggregatum group).
[So] Source:J Agric Food Chem;61(31):7440-5, 2013 Aug 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The n-butanol extract of shallot basal plates and roots showed antifungal activity against plant pathogenic fungi. The purified compounds from the extract were examined for antifungal activity to determine the predominant antifungal compounds in the extract. Two major antifungal compounds purified were determined to be alliospiroside A (ALA) and alliospiroside B. ALA had prominent antifungal activity against a wide range of fungi. The products of acid hydrolysis of ALA showed a reduced antifungal activity, suggesting that the compound's sugar chain is essential for its antifungal activity. Fungal cells treated with ALA showed rapid production of reactive oxygen species. The fungicidal action of ALA was partially inhibited by a superoxide scavenger, Tiron, suggesting that superoxide anion generation in the fungal cells may be related to the compound's action. Inoculation experiments showed that ALA protected strawberry plants against Colletotrichum gloeosporioides , indicating that ALA has the potential to control anthracnose of the plant.
[Mh] Termos MeSH primário: Fungicidas Industriais/química
Fungicidas Industriais/farmacologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Saponinas/química
Saponinas/farmacologia
Cebolinha Branca/química
[Mh] Termos MeSH secundário: Colletotrichum/efeitos dos fármacos
Colletotrichum/fisiologia
Fragaria/microbiologia
Fungos/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Doenças das Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Plant Extracts); 0 (Saponins)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:131021
[Lr] Data última revisão:
131021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131022
[St] Status:MEDLINE
[do] DOI:10.1021/jf401720q


  10 / 36 MEDLINE  
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[PMID]:22987571
[Au] Autor:Rahman MM; Park JH; Abd El-Aty AM; Choi JH; Bae HR; Yang A; Park KH; Shim JH
[Ad] Endereço:Natural Products Chemistry Laboratory, Biotechnology Research Institute, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, Republic of Korea.
[Ti] Título:Single-step modified QuEChERS for determination of chlorothalonil in shallot (Allium ascalonicum) using GC-µECD and confirmation via mass spectrometry.
[So] Source:Biomed Chromatogr;27(4):416-21, 2013 Apr.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A single extraction method was developed for chlorothalonil in shallot using gas chromatography with an electron capture detector (GC-µECD). Samples were extracted with single-step modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method using ethyl acetate as an extraction solvent. Significant matrix effects were observed, and the calibration curve was constructed from the matrix. The linearity of the analytical response across the studied range of concentrations (0.01-1.00 mg/L) was excellent, obtaining a correlation coefficient (r(2) ) of 0.996. >0.996. Recovery studies were carried out on spiked shallot blank samples, at two concentration levels (0.4 and 2.0 mg/kg) with three replicates performed at each level. Mean recoveries of 97.2-104.9% with RSDs of 1.3-2.7% were obtained. The method is demonstrated to be suitable for the determination of chlorothalonil in shallot. The dissipation rates of chlorothalonil were described using first-order kinetics, and its half-life was 2.8 days. Based on the dissipation pattern of the pesticide residues, the pre-harvest residue limit (PHRL) was also calculated. Residues were confirmed via mass spectrometry.
[Mh] Termos MeSH primário: Fungicidas Industriais/análise
Cromatografia Gasosa-Espectrometria de Massas/métodos
Nitrilos/análise
Resíduos de Praguicidas/análise
Cebolinha Branca/química
[Mh] Termos MeSH secundário: Acetatos/química
Fungicidas Industriais/isolamento & purificação
Nitrilos/isolamento & purificação
Resíduos de Praguicidas/isolamento & purificação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetates); 0 (Fungicides, Industrial); 0 (Nitriles); 0 (Pesticide Residues); 76845O8NMZ (ethyl acetate); J718M71A7A (tetrachloroisophthalonitrile)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120919
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.2808



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