Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.100.050.295 [Categoria DeCS]
Referências encontradas : 148 [refinar]
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[PMID]:27938939
[Au] Autor:Mi X; Liu X; Yan H; Liang L; Zhou X; Yang J; Si H; Zhang N
[Ad] Endereço:Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republi
[Ti] Título:Expression of the Galanthus nivalis agglutinin (GNA) gene in transgenic potato plants confers resistance to aphids.
[So] Source:C R Biol;340(1):7-12, 2017 Jan.
[Is] ISSN:1768-3238
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Aphids, the largest group of sap-sucking pests, cause significant yield losses in agricultural crops worldwide every year. The massive use of pesticides to combat this pest causes severe damage to the environment, putting in risk the human health. In this study, transgenic potato plants expressing Galanthus nivalis agglutinin (GNA) gene were developed using CaMV 35S and ST-LS1 promoters generating six transgenic lines (35S1-35S3 and ST1-ST3 corresponding to the first and second promoter, respectively). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the GNA gene was expressed in leaves, stems and roots of transgenic plants under the control of the CaMV 35S promoter, while it was only expressed in leaves and stems under the control of the ST-LS1 promoter. The levels of aphid mortality after 5 days of the inoculation in the assessed transgenic lines ranged from 20 to 53.3%. The range of the aphid population in transgenic plants 15 days after inoculation was between 17.0±1.43 (ST2) and 36.6±0.99 (35S3) aphids per plant, which corresponds to 24.9-53.5% of the aphid population in non-transformed plants. The results of our study suggest that GNA expressed in transgenic potato plants confers a potential tolerance to aphid attack, which appears to be an alternative against the use of pesticides in the future.
[Mh] Termos MeSH primário: Aglutininas/genética
Afídeos
Galanthus/genética
Controle Biológico de Vetores/métodos
Plantas Geneticamente Modificadas/genética
Solanum tuberosum/genética
[Mh] Termos MeSH secundário: Animais
Vetores Genéticos
Doenças das Plantas
Folhas de Planta/metabolismo
Caules de Planta/metabolismo
População
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agglutinins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27975363
[Au] Autor:Koch B; Büttner S; Geiger H
[Ti] Título:[Reducing viral load in life-threatening viral diseases using snowdrops].
[Ti] Título:Schneeglöckchen zur Senkung der Viruslast bei lebensbedrohlichen Viruserkrankungen..
[So] Source:Dtsch Med Wochenschr;141(25):1868-1871, 2016 Dec.
[Is] ISSN:1439-4413
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:In the case of life-threatening viral diseases, viral load is associated with mortality. A new and innovative therapeutic approach is the reduction of viral load by extracorporeal elimination without simultaneously weakening the immune system by removing specific antibodies. Basis of this therapy is a modified plasma filter coated with a lectin derived from the snowdrop.
[Mh] Termos MeSH primário: Galanthus/química
Lectinas/química
Plasmaferese/métodos
Carga Viral/efeitos dos fármacos
Viroses/prevenção & controle
Viroses/virologia
[Mh] Termos MeSH secundário: Anticorpos Antivirais/sangue
Anticorpos Antivirais/química
Anticorpos Antivirais/isolamento & purificação
Cuidados Críticos/métodos
Medicina Baseada em Evidências
Seres Humanos
Resultado do Tratamento
Viroses/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Lectins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE


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[PMID]:27252378
[Au] Autor:Kilgore MB; Holland CK; Jez JM; Kutchan TM
[Ad] Endereço:From the Donald Danforth Plant Science Center, St. Louis, Missouri 63132 and the Department of Biology, Washington University in St. Louis, St. Louis, Missouri 63130.
[Ti] Título:Identification of a Noroxomaritidine Reductase with Amaryllidaceae Alkaloid Biosynthesis Related Activities.
[So] Source:J Biol Chem;291(32):16740-52, 2016 08 05.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amaryllidaceae alkaloids are a large group of plant natural products with over 300 documented structures and diverse biological activities. Several groups of Amaryllidaceae alkaloids including the hemanthamine- and crinine-type alkaloids show promise as anticancer agents. Two reduction reactions are required for the production of these compounds: the reduction of norcraugsodine to norbelladine and the reduction of noroxomaritidine to normaritidine, with the enantiomer of noroxomaritidine dictating whether the derivatives will be the crinine-type or hemanthamine-type. It is also possible for the carbon-carbon double bond of noroxomaritidine to be reduced, forming the precursor for maritinamine or elwesine depending on the enantiomer reduced to an oxomaritinamine product. In this study, a short chain alcohol dehydrogenase/reductase that co-expresses with the previously discovered norbelladine 4'-O-methyltransferase from Narcissus sp. and Galanthus spp. was cloned and expressed in Escherichia coli Biochemical analyses and x-ray crystallography indicates that this protein functions as a noroxomaritidine reductase that forms oxomaritinamine from noroxomaritidine through a carbon-carbon double bond reduction. The enzyme also reduces norcraugsodine to norbelladine with a 400-fold lower specific activity. These studies identify a missing step in the biosynthesis of this pharmacologically important class of plant natural products.
[Mh] Termos MeSH primário: Alcaloides de Amaryllidaceae/química
Galanthus/enzimologia
Narcissus/enzimologia
Oxirredutases/química
Proteínas de Plantas/química
[Mh] Termos MeSH secundário: Alcaloides de Amaryllidaceae/metabolismo
Galanthus/genética
Narcissus/genética
Oxirredução
Oxirredutases/genética
Oxirredutases/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Amaryllidaceae Alkaloids); 0 (Plant Proteins); 0 (Recombinant Proteins); EC 1.- (Oxidoreductases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160603
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.717827


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[PMID]:25641865
[Au] Autor:Liu SM; Li J; Zhu JQ; Wang XW; Wang CS; Liu SS; Chen XX; Li S
[Ad] Endereço:Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Transgenic plants expressing the AaIT/GNA fusion protein show increased resistance and toxicity to both chewing and sucking pests.
[So] Source:Insect Sci;23(2):265-76, 2016 Apr.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:The adoption of pest-resistant transgenic plants to reduce yield losses and decrease pesticide use has been successful. To achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, Arabidopsis, and rice plants expressing the fusion protein, AaIT/GNA, in which an insecticidal scorpion venom neurotoxin (Androctonus australis toxin, AaIT) is fused to snowdrop lectin (Galanthus nivalis agglutinin, GNA). Compared with transgenic tobacco and Arabidopsis plants expressing AaIT or GNA, transgenic plants expressing AaIT/GNA exhibited increased resistance and toxicity to one chewing pest, the cotton bollworm, Helicoverpa armigera. Transgenic tobacco and rice plants expressing AaIT/GNA showed increased resistance and toxicity to two sucking pests, the whitefly, Bemisia tabaci, and the rice brown planthopper, Nilaparvata lugens, respectively. Moreover, in the field, transgenic rice plants expressing AaIT/GNA exhibited a significant improvement in grain yield when infested with N. lugens. This study shows that expressing the AaIT/GNA fusion protein in transgenic plants can be a useful approach for controlling pests, particularly sucking pests which are not susceptible to the toxin in Bt crops.
[Mh] Termos MeSH primário: Antibiose
Arabidopsis/fisiologia
Herbivoria/efeitos dos fármacos
Insetos/fisiologia
Oryza/fisiologia
Venenos de Escorpião/farmacologia
Tabaco/fisiologia
[Mh] Termos MeSH secundário: Animais
Arabidopsis/genética
Galanthus/química
Hemípteros/crescimento & desenvolvimento
Hemípteros/fisiologia
Insetos/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/fisiologia
Lectinas de Ligação a Manose/genética
Lectinas de Ligação a Manose/farmacologia
Mariposas/crescimento & desenvolvimento
Mariposas/fisiologia
Ninfa/crescimento & desenvolvimento
Ninfa/fisiologia
Oryza/genética
Lectinas de Plantas/genética
Lectinas de Plantas/farmacologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/fisiologia
Venenos de Escorpião/genética
Escorpiões/química
Tabaco/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Mannose-Binding Lectins); 0 (Plant Lectins); 0 (Scorpion Venoms); 0 (snowdrop lectin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150203
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12203


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[PMID]:26407826
[Au] Autor:Hu B; Du T; Li C; Luo S; Liu Y; Huang X; Hu Q
[Ad] Endereço:1​State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China 2​University of Chinese Academy of Sciences, Beijing 100049, PR China.
[Ti] Título:Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin.
[So] Source:J Gen Virol;96(12):3660-6, 2015 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human immunodeficiency virus type 1 (HIV-1) transmission often results from infection by a single transmitted/founder (T/F) virus. Here, we investigated the sensitivity of T/F HIV-1 envelope glycoproteins (Envs) to microbicide candidate carbohydrate-binding agents (CBAs) griffithsin (GRFT), cyanovirin-N (CV-N) and Galanthus nivalis agglutinin (GNA), showing that T/F Envs demonstrated different sensitivity to CBAs, with IC50 values ranging from 0.006 ± 0.0003 to >10 nM for GRFT, from 0.6 ± 0.2 to 28.9 ± 2.9 nM for CV-N and from 1.3 ± 0.2 to >500 nM for GNA. We further revealed that deglycosylation at position 295 or 448 decreased the sensitivity of T/F Env to GRFT, and at 339 to both CV-N and GNA. Mutation of all the three glcyans rendered a CBA-sensitive T/F Env largely resistant to GRFT, indicating that the sensitivity of T/F Env to GRFT is mainly determined by glycans at 295, 339 and 448. Our study identified specific T/F Env residues associated with CBA sensitivity.
[Mh] Termos MeSH primário: Aglutininas/farmacologia
Proteínas de Bactérias/farmacologia
Proteínas de Transporte/farmacologia
Galanthus/química
HIV-1/fisiologia
Lectinas de Plantas/farmacologia
Proteínas do Envelope Viral/metabolismo
[Mh] Termos MeSH secundário: Aglutininas/química
Sequência de Aminoácidos
Fármacos Anti-HIV/química
Fármacos Anti-HIV/farmacologia
Regulação Viral da Expressão Gênica
Proteína gp120 do Envelope de HIV
Seres Humanos
Dados de Sequência Molecular
Sensibilidade e Especificidade
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Agglutinins); 0 (Anti-HIV Agents); 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (HIV Envelope Protein gp120); 0 (Plant Lectins); 0 (Viral Envelope Proteins); 0 (griffithsin protein, Griffithsia); 184539-38-6 (cyanovirin N)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:151221
[Lr] Data última revisão:
151221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150927
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000299


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[PMID]:25764435
[Au] Autor:Turi CE; Axwik KE; Smith A; Jones AM; Saxena PK; Murch SJ
[Ad] Endereço:a Biology; University of British Columbia; Okanagan Campus; Kelowna, BC Canada.
[Ti] Título:Galanthamine, an anti-cholinesterase drug, effects plant growth and development in Artemisia tridentata Nutt. via modulation of auxin and neurotransmitter signaling.
[So] Source:Plant Signal Behav;9(4):e28645, 2014.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Galanthamine is a naturally occurring acetylcholinesterase (AchE) inhibitor that has been well established as a drug for treatment of mild to moderate Alzheimer disease, but the role of the compound in plant metabolism is not known. The current study was designed to investigate whether galanthamine could redirect morphogenesis of Artemisia tridentata Nutt. cultures by altering concentration of endogenous neurosignaling molecules acetylcholine (Ach), auxin (IAA), melatonin (Mel), and serotonin (5HT). Exposure of axenic A. tridentata cultures to 10 µM galanthamine decreased the concentration of endogenous Ach, IAA, MEL, and AchE, and altered plant growth in a manner reminiscent of 2-4D toxicity. Galanthamine itself demonstrated IAA activity in an oat coleoptile elongation bioassay, 20 µM galanthamine showed no significant difference compared with 5 µM IAA or 5 µM 1-Naphthaleneacetic acid (NAA). Metabolomic analysis detected between 20,921 to 27,891 compounds in A. tridentata plantlets and showed greater commonality between control and 5 µM treatments. Furthermore, metabolomic analysis putatively identified coumarins scopoletin/isoscopoletin, and scopolin in A. tridentata leaf extracts and these metabolites linearly increased in response to galanthamine treatments. Overall, these data indicate that galanthamine is an allelopathic phytochemical and support the hypothesis that neurologically active compounds in plants help ensure plant survival and adaptation to environmental challenges.
[Mh] Termos MeSH primário: Acetilcolina/metabolismo
Acetilcolinesterase/metabolismo
Artemisia/efeitos dos fármacos
Inibidores da Colinesterase/farmacologia
Galantamina/farmacologia
Ácidos Indolacéticos/metabolismo
Desenvolvimento Vegetal/efeitos dos fármacos
[Mh] Termos MeSH secundário: Artemisia/crescimento & desenvolvimento
Artemisia/metabolismo
Cumarínicos/metabolismo
Galanthus/química
Glucosídeos/metabolismo
Melatonina/metabolismo
Metaboloma
Metabolômica
Ácidos Naftalenoacéticos/farmacologia
Neurotransmissores/metabolismo
Oryza/efeitos dos fármacos
Oryza/crescimento & desenvolvimento
Extratos Vegetais/farmacologia
Reguladores de Crescimento de Planta/metabolismo
Reguladores de Crescimento de Planta/farmacologia
Folhas de Planta/metabolismo
Escopoletina/análogos & derivados
Escopoletina/metabolismo
Serotonina/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Coumarins); 0 (Glucosides); 0 (Indoleacetic Acids); 0 (Naphthaleneacetic Acids); 0 (Neurotransmitter Agents); 0 (Plant Extracts); 0 (Plant Growth Regulators); 0 (isoscopoletin); 0D3Q044KCA (Galantamine); 1Y49270PY8 (scopolin); 333DO1RDJY (Serotonin); 33T7G7757C (1-naphthaleneacetic acid); EC 3.1.1.7 (Acetylcholinesterase); JL5DK93RCL (Melatonin); KLF1HS0SXJ (Scopoletin); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150313
[St] Status:MEDLINE
[do] DOI:10.4161/psb.28645


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[PMID]:25233596
[Au] Autor:Kaya GI; Polat DC; Sarikaya B; Onur MA; Somer NU
[Ti] Título:Quantititative determination of lycorine and galanthamine in Galanthus trojanus and G. cilicicus by HPLC-DAD.
[So] Source:Nat Prod Commun;9(8):1157-8, 2014 Aug.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lycorine and galanthamine have various biological activities. A reliable HPLC method coupled with DAD detection was developed and validated for the determination of galanthamine and lycorine in Galanthus trojanus and G. cilicicus. A simple method for the extraction of the alkaloids in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut). This method was applied to the aerial parts and bulbs of G. trojanus and G. cilicicus (Amaryllidaceae) collected during the flowering season. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5) applied at a flow rate of 1 mL min(-1) and using a diode array detector. Validation procedures showed that the method was specific, accurate and precise. The highest amount of lycorine (0.012%) was detected in the bulbs of G. trojanus collected from Can (Canakkale), whereas the aerial parts of this species collected from Bayramiç (Canakkale) was not found to contain this alkaloid. In G. cilicicus samples, lycorine was only determined in the bulbs, giving yields of 0.004%; galanthamine yields were between 0.015-0.016%, but none of the G. trojanus samples contained this latter alkaloid.
[Mh] Termos MeSH primário: Alcaloides de Amaryllidaceae/análise
Galantamina/análise
Galanthus/química
Fenantridinas/análise
Extratos Vegetais/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Flores/química
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amaryllidaceae Alkaloids); 0 (Phenanthridines); 0 (Plant Extracts); 0D3Q044KCA (Galantamine); I9Q105R5BU (lycorine)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140919
[Lr] Data última revisão:
140919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140920
[St] Status:MEDLINE


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[PMID]:25050493
[Ti] Título:Holy Moly: Hermes' anticholinesterase antidote.
[So] Source:Anesthesiology;121(2):371, 2014 Aug.
[Is] ISSN:1528-1175
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antídotos/história
Inibidores da Colinesterase/envenenamento
Reativadores da Colinesterase/história
Galantamina/história
Galanthus/química
[Mh] Termos MeSH secundário: Antídotos/química
Antídotos/farmacologia
Reativadores da Colinesterase/química
Reativadores da Colinesterase/farmacologia
Flores/química
Galantamina/farmacologia
História do Século XIX
Seres Humanos
Raízes de Plantas/química
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidotes); 0 (Cholinesterase Inhibitors); 0 (Cholinesterase Reactivators); 0D3Q044KCA (Galantamine)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140723
[Lr] Data última revisão:
140723
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:140723
[St] Status:MEDLINE
[do] DOI:10.1097/01.anes.0000451816.76346.cd


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[PMID]:24898372
[Au] Autor:Nakasu EY; Williamson SM; Edwards MG; Fitches EC; Gatehouse JA; Wright GA; Gatehouse AM
[Ad] Endereço:School of Biology, Newcastle Institute for Research and Sustainability, Newcastle University, Newcastle upon Tyne NE1 7RU, UK Capes Foundation, Ministry of Education of Brazil, Caixa Postal 250, Brasília 70040-020, Brazil.
[Ti] Título:Novel biopesticide based on a spider venom peptide shows no adverse effects on honeybees.
[So] Source:Proc Biol Sci;281(1787), 2014 Jul 22.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Evidence is accumulating that commonly used pesticides are linked to decline of pollinator populations; adverse effects of three neonicotinoids on bees have led to bans on their use across the European Union. Developing insecticides that pose negligible risks to beneficial organisms such as honeybees is desirable and timely. One strategy is to use recombinant fusion proteins containing neuroactive peptides/proteins linked to a 'carrier' protein that confers oral toxicity. Hv1a/GNA (Galanthus nivalis agglutinin), containing an insect-specific spider venom calcium channel blocker (ω-hexatoxin-Hv1a) linked to snowdrop lectin (GNA) as a 'carrier', is an effective oral biopesticide towards various insect pests. Effects of Hv1a/GNA towards a non-target species, Apis mellifera, were assessed through a thorough early-tier risk assessment. Following feeding, honeybees internalized Hv1a/GNA, which reached the brain within 1 h after exposure. However, survival was only slightly affected by ingestion (LD50>100 µg bee(-1)) or injection of fusion protein. Bees fed acute (100 µg bee(-1)) or chronic (0.35 mg ml(-1)) doses of Hv1a/GNA and trained in an olfactory learning task had similar rates of learning and memory to no-pesticide controls. Larvae were unaffected, being able to degrade Hv1a/GNA. These tests suggest that Hv1a/GNA is unlikely to cause detrimental effects on honeybees, indicating that atracotoxins targeting calcium channels are potential alternatives to conventional pesticides.
[Mh] Termos MeSH primário: Abelhas/efeitos dos fármacos
Bloqueadores dos Canais de Cálcio/toxicidade
Inseticidas/toxicidade
Lectinas de Ligação a Manose/toxicidade
Lectinas de Plantas/toxicidade
Venenos de Aranha/toxicidade
[Mh] Termos MeSH secundário: Animais
Abelhas/crescimento & desenvolvimento
Bloqueadores dos Canais de Cálcio/metabolismo
Galanthus/química
Inseticidas/metabolismo
Larva/efeitos dos fármacos
Aprendizagem/efeitos dos fármacos
Lectinas de Ligação a Manose/genética
Lectinas de Ligação a Manose/metabolismo
Lectinas de Plantas/genética
Lectinas de Plantas/metabolismo
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes de Fusão/toxicidade
Venenos de Aranha/genética
Venenos de Aranha/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Calcium Channel Blockers); 0 (Insecticides); 0 (Mannose-Binding Lectins); 0 (Plant Lectins); 0 (Recombinant Fusion Proteins); 0 (Spider Venoms); 0 (snowdrop lectin)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140606
[St] Status:MEDLINE


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[PMID]:24873910
[Au] Autor:Resetár A; Demeter Z; Ficsor E; Balázs A; Mosolygó A; Szoke E; Gonda S; Papp L; Surányi G; Máthé C
[Ad] Endereço:University of Debrecen Department of Botany, Faculty of Science and Technology Egyetem tér 1 H-4032 Debrecen Hungary.
[Ti] Título:Growth regulator requirement for in vitro embryogenic cultures of snowdrop (Galanthus nivalis L.) suitable for germplasm preservation.
[So] Source:Acta Biol Hung;65(2):165-77, 2014 Jun.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:In this study, we report on the production of bulb scale-derived tissue cultures capable of efficient shoot and plant regeneration in three genotypes of snowdrop (Galanthus nivalis L., Amaryllidaceae), a protected ornamental plant. For culture line A, high auxin and low cytokinin concentration is required for callus production and plant regeneration. The type of auxin is of key importance: α-naphthaleneacetic acid (NAA) in combination with indole-3-acetic acid (IAA) at concentrations of 2 mg L-1 or 2-10 mg L-1 NAA with 1 mg L-1 N6-benzyladenine (BA), a cytokinin on full-strength media are required for regeneration. Cultures showing regeneration were embryogenic. When lines B and C were induced and maintained with 2 mg L-1 NAA and 1 mg L-1 BA, they produced mature bulblets with shoots, without roots. Line A produced immature bulblets with shoots under the above culture condition. Amplified Fragment Length Polymorphism (AFLP) analysis showed that (i) genetic differences between line A and its bulb explants were not significant, therefore these tissue cultures are suitable for germplasm preservation, and (ii) different morphogenetic responses of lines A, B and C originated from genetic differences. Culture line A is suitable for field-growing, cultivation and germplasm preservation of G. nivalis and for the production of Amaryllidaceae alkaloids.
[Mh] Termos MeSH primário: Galanthus/efeitos dos fármacos
Reguladores de Crescimento de Planta/farmacologia
Sementes/efeitos dos fármacos
[Mh] Termos MeSH secundário: Compostos de Benzil
Galanthus/genética
Galanthus/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Genótipo
Ácidos Indolacéticos/farmacologia
Cinetina/farmacologia
Ácidos Naftalenoacéticos/farmacologia
Fenótipo
Desenvolvimento Vegetal/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/crescimento & desenvolvimento
Purinas
Regeneração/efeitos dos fármacos
Sementes/crescimento & desenvolvimento
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzyl Compounds); 0 (Indoleacetic Acids); 0 (Naphthaleneacetic Acids); 0 (Plant Growth Regulators); 0 (Purines); 33T7G7757C (1-naphthaleneacetic acid); 6U1S09C61L (indoleacetic acid); KXG6A989PS (benzylaminopurine); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140531
[St] Status:MEDLINE
[do] DOI:10.1556/ABiol.65.2014.2.5



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