Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.618.100.060.032 [Categoria DeCS] Referências encontradas : 290 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28827111 [Au] Autor: Pereira GM; Ribeiro MG; da Silva BP; Parente JP [Ad] Endereço: Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, P.O. Box 68045, CEP 21941-971 Rio de Janeiro, Brazil. [Ti] Título: Structural characterization of a new steroidal saponin from Agave angustifolia var. Marginata and a preliminary investigation of its in vivo antiulcerogenic activity and in vitro membrane permeability property. [So] Source: Bioorg Med Chem Lett;27(18):4345-4349, 2017 09 15. [Is] ISSN: 1464-3405 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A new furostane steroidal saponin was isolated from the leaves of Agave angustifolia var. marginata. On the basis of chemical conversions and spectroscopic analyses, its structure was established as 3-[O-ß-d-glucopyranosyl-(1→3)-O-ß-d-glucopyranosyl-(1→3)-O]-[O-6-deoxy-α-l-mannopyranosyl-(1→4)-ß-d-xylopyranosyl-(1→2)-O-ß-d-glucopyranosyl-(1→4)-ß-d-galactopyranosyl)oxy]-(3ß,5α,22α,25R)-26-(ß-d-glucopyranosyloxy)-22-methoxy-furostane (1). Results of preliminary biological investigations indicated that compound 1 showed significant protective effects against induced gastric ulcers using in vivo experimental models and demonstrated negligible toxicity on membrane integrity in the in vitro assays. [Mh] Termos MeSH primário: Agave/química Antiulcerosos/farmacologia Saponinas/farmacologia Úlcera Gástrica/tratamento farmacológico [Mh] Termos MeSH secundário: Animais Antiulcerosos/química Antiulcerosos/isolamento & purificação Permeabilidade da Membrana Celular/efeitos dos fármacos Relação Dose-Resposta a Droga Seres Humanos Masculino Camundongos Estrutura Molecular Folhas de Planta/química Saponinas/química Saponinas/isolamento & purificação Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Ulcer Agents); 0 (Saponins) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171125 [Lr] Data última revisão: 171125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170823 [St] Status: MEDLINE

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[PMID]: 28764075 [Au] Autor: Moreno-Vilet L; Bostyn S; Flores-Montaño JL; Camacho-Ruiz RM [Ad] Endereço: CONACYT- Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Guadalajara, Jalisco, Mexico. [Ti] Título: Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans. [So] Source: Food Chem;237:833-840, 2017 Dec 15. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7°C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36mL/min. The exclusion range is from 1 to 49 of polymerization degree (180-7966Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity. [Mh] Termos MeSH primário: Agave Cromatografia Líquida de Alta Pressão [Mh] Termos MeSH secundário: Cromatografia em Gel Frutanos Peso Molecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fructans) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170803 [St] Status: MEDLINE

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[PMID]: 28497409 [Au] Autor: Lledías F; Hernández F; Rivas V; García-Mendoza A; Cassab GI; Nieto-Sotelo J [Ad] Endereço: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad # 2001, Col. Chamilpa C.P., 62210, Cuernavaca, Morelos, Mexico. [Ti] Título: A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis. [So] Source: Protein J;36(4):308-321, 2017 Aug. [Is] ISSN: 1875-8355 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Crassulacean acid metabolism plants have some morphological features, such as succulent and reduced leaves, thick cuticles, and sunken stomata that help them prevent excessive water loss and irradiation. As molecular constituents of these morphological adaptations to xeric environments, succulent plants produce a set of specific compounds such as complex polysaccharides, pigments, waxes, and terpenoids, to name a few, in addition to uncharacterized proteases. Since all these compounds interfere with the analysis of proteins by electrophoretic techniques, preparation of high quality samples from these sources represents a real challenge. The absence of adequate protocols for protein extraction has restrained the study of this class of plants at the molecular level. Here, we present a rapid and reliable protocol that could be accomplished in 1 h and applied to a broad range of plants with reproducible results. We were able to obtain well-resolved SDS/PAGE protein patterns in extracts from different members of the subfamilies Agavoideae (Agave, Yucca, Manfreda, and Furcraea), Nolinoideae (Dasylirion and Beucarnea), and the Cactaceae family. This method is based on the differential solubility of contaminants and proteins in the presence of acetone and pH-altered solutions. We speculate about the role of saponins and high molecular weight carbohydrates to produce electrophoretic-compatible samples. A modification of the basic protocol allowed the analysis of samples by bidimensional electrophoresis (2DE) for proteomic analysis. Furostanol glycoside 26-O-ß-glucosidase (an enzyme involved in steroid saponin synthesis) was successfully identified by mass spectrometry analysis and de novo sequencing of a 2DE spot from an Agave attenuata sample. [Mh] Termos MeSH primário: Extração Líquido-Líquido/métodos Folhas de Planta/química Proteínas de Plantas/isolamento & purificação Proteômica/métodos beta-Glucosidase/isolamento & purificação [Mh] Termos MeSH secundário: Acetona/química Agave/química Asparagaceae/química Cactaceae/química Eletroforese em Gel Bidimensional Espectrometria de Massas Solventes/química Yucca/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Proteins); 0 (Solvents); 1364PS73AF (Acetone); EC 3.2.1.- (furostanol glycoside 26-O-beta-glucosidase); EC 3.2.1.21 (beta-Glucosidase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 171020 [Lr] Data última revisão: 171020 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170513 [St] Status: MEDLINE [do] DOI: 10.1007/s10930-017-9720-3

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[PMID]: 28325555 [Au] Autor: Rios-González LJ; Morales-Martínez TK; Rodríguez-Flores MF; Rodríguez-De la Garza JA; Castillo-Quiroz D; Castro-Montoya AJ; Martinez A [Ad] Endereço: Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico. Electronic address: leopoldo.rios@uadec.edu.mx. [Ti] Título: Autohydrolysis pretreatment assessment in ethanol production from agave bagasse. [So] Source: Bioresour Technol;242:184-190, 2017 Oct. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6w/v ratio (solid:liquid) and 200rpm. The solids obtained under the selected autohydrolysis conditions were subjected to enzymatic hydrolysis with a commercial cellulase cocktail, and the enzymatic hydrolysate was fermented using Saccharomyces cerevisiae. The results obtained from the pretreatment process showed that the glucan content in the pretreated solid was mostly preserved, and an increase in the digestibility was observed for the case with a SF of 4.13 (190°C, 30min). Enzymatic hydrolysis of the pretreated solids showed a yield of 74.3%, with a glucose concentration of 126g/L, resulting in 65.26g/L of ethanol after 10h of fermentation, which represent a 98.4% conversion according to the theoretical ethanol yield value. [Mh] Termos MeSH primário: Agave Celulose [Mh] Termos MeSH secundário: Celulase Etanol Fermentação Hidrólise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 3K9958V90M (Ethanol); 9004-34-6 (Cellulose); 9006-97-7 (bagasse); EC 3.2.1.4 (Cellulase) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171106 [Lr] Data última revisão: 171106 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170323 [St] Status: MEDLINE

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[PMID]: 28293641 [Au] Autor: Rivera-Huerta M; Lizárraga-Grimes VL; Castro-Torres IG; Tinoco-Méndez M; Macías-Rosales L; Sánchez-Bartéz F; Tapia-Pérez GG; Romero-Romero L; Gracia-Mora MI [Ad] Endereço: Unidad de Investigación Preclínica (UNIPREC), Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. [Ti] Título: Functional Effects of Prebiotic Fructans in Colon Cancer and Calcium Metabolism in Animal Models. [So] Source: Biomed Res Int;2017:9758982, 2017. [Is] ISSN: 2314-6141 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Inulin-type fructans are polymers of fructose molecules and are known for their capacity to enhance absorption of calcium and magnesium, to modulate gut microbiota and energy metabolism, and to improve glycemia. We evaluated and compared the effects of Chicory inulin "Synergy 1®" and inulin from Mexican agave "Metlin®" in two experimental models of colon cancer and bone calcium metabolism in mice and rats. Inulins inhibited the development of dextran sulfate sodium-induced colitis and colon cancer in mice; these fructans reduced the concentration of tumor necrosis factor alpha and prevented the formation of intestinal polyps, villous atrophy, and lymphoid hyperplasia. On the other hand, inulin treatments significantly increased bone densitometry (femur and vertebra) in ovariectomized rats without altering the concentration of many serum biochemical parameters and urinary parameters. Histopathology results were compared between different experimental groups. There were no apparent histological changes in rats treated with inulins and a mixture of inulins-isoflavones. Our results showed that inulin-type fructans have health-promoting properties related to enhanced calcium absorption, potential anticancer properties, and anti-inflammatory effects. The use of inulin as a prebiotic can improve health and prevent development of chronic diseases such as cancer and osteoporosis. [Mh] Termos MeSH primário: Cálcio na Dieta/metabolismo Neoplasias do Colo/tratamento farmacológico Frutanos/química Prebióticos [Mh] Termos MeSH secundário: Agave Animais Osso e Ossos/efeitos dos fármacos Densitometria Suplementos Nutricionais Modelos Animais de Doenças Ensaio de Imunoadsorção Enzimática Feminino Absorção Intestinal Inulina/química Masculino Camundongos Camundongos Endogâmicos BALB C Fitoterapia Extratos Vegetais/química Ratos Ratos Wistar [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Calcium, Dietary); 0 (Fructans); 0 (Plant Extracts); 0 (Prebiotics); 9005-80-5 (Inulin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170417 [Lr] Data última revisão: 170417 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170316 [St] Status: MEDLINE [do] DOI: 10.1155/2017/9758982

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[PMID]: 28106207 [Au] Autor: Bihter Gürler E; Özbeyli D; Buzcu H; Bayraktar S; Carus I; Dag B; Geris Y; Jeral S; Yegen BÇ [Ad] Endereço: Marmara University, School of Medicine, Department of Physiology, Istanbul, Turkey. byegen@marmara.edu.tr. [Ti] Título: Natural sweetener agave inhibits gastric emptying in rats by a cholecystokinin-2- and glucagon like peptide-1 receptor-dependent mechanism. [So] Source: Food Funct;8(2):741-745, 2017 Feb 22. [Is] ISSN: 2042-650X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Low-calorie sweeteners are considered to be beneficial in calorie control, but the impact of these sweeteners on gastric emptying is not well described. The purpose of this study was to compare the gastric emptying rate of agave nectar with those of glucose and fructose, and to evaluate the interaction of cholecystokinin (CCK)-1, CCK-2 and glucagon-like peptide-1 (GLP-1) receptors in agave-induced alterations in gastric emptying. Female Sprague-Dawley rats were fitted with gastric cannulas. Following the recovery, the gastric emptying rates of glucose, fructose and agave at 12.5%, 15% or 50% concentrations were measured and compared with that of saline. GLP-1 receptor antagonist exendin fragment 9-39 (30 µg kg ), CCK-1 receptor antagonist devazepide (1 mg kg ) or gastrin/CCK-2 receptor antagonist YM022 (1 mg kg ) was injected subcutaneously 1 min before the emptying of glucose, fructose or agave at their 50% concentrations. When compared with saline emptying, gastric emptying of glucose was significantly delayed at its 25% and 50% concentrations, but the emptying of 12.5% glucose was not different from that of saline. Agave emptying, which was delayed with respect to saline emptying, was not altered by CCK-1 receptor blockade; but agave emptied from the stomach as rapidly as saline following the blockade of either CCK-2 or GLP-1 receptors. The findings demonstrate that the inhibitory effect of agave on gastric emptying is mediated by both CCK-2 and GLP-1 receptors, suggesting that natural sweeteners including agave may have satiating effects through the inhibition of gastric motility via enteroendocrine mechanisms. [Mh] Termos MeSH primário: Agave/metabolismo Colecistocinina/metabolismo Esvaziamento Gástrico Receptor do Peptídeo Semelhante ao Glucagon 1/metabolismo Extratos Vegetais/metabolismo Estômago/fisiologia Edulcorantes/metabolismo [Mh] Termos MeSH secundário: Agave/química Animais Feminino Frutose/metabolismo Glucose/metabolismo Ratos Ratos Sprague-Dawley [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Glucagon-Like Peptide-1 Receptor); 0 (Plant Extracts); 0 (Sweetening Agents); 30237-26-4 (Fructose); 9011-97-6 (Cholecystokinin); IY9XDZ35W2 (Glucose) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170327 [Lr] Data última revisão: 170327 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170121 [St] Status: MEDLINE [do] DOI: 10.1039/c6fo01438k

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[PMID]: 28005446 [Au] Autor: Contreras-Haro B; Robles-Cervantes JA; Gonzalez-Ortiz M; Martinez-Abundis E; Espinel-Bermudez C; Gallegos-Arreola MP; Morgado-Castillo KC [Ad] Endereço: 1 Medical Research Unit in Clinical Epidemiology, Specialties Hospital, Medical Unit of High Speciality, West National Medical Center, Mexican Institute of Social Security , Guadalajara. México. [Ti] Título: The Effect of Agave tequilana Weber Inulin on Postprandial Ghrelin Concentration in Obese Patients. [So] Source: J Med Food;20(2):197-199, 2017 Feb. [Is] ISSN: 1557-7600 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This study was performed to investigate the effect of Agave tequilana Weber inulin on postprandial ghrelin levels in obese patients. A randomized, double-blind, cross-over design was performed. A total of 14 patients were allocated into two groups: one group received a drink that contained 500 mL lemon water, 24 g of A. tequilana Weber inulin, and 75 g glucose and the other group received a placebo drink with 500 mL lemon drink and 75 g of glucose. After a 7-day washout period, the groups were crossed. The primary outcome measure was postprandial ghrelin levels between minute 240 and minute 270. A. tequilana Weber inulin did not change postprandial ghrelin concentration in obese patients. [Mh] Termos MeSH primário: Agave/química Grelina/sangue Inulina/administração & dosagem Obesidade/tratamento farmacológico Extratos Vegetais/administração & dosagem [Mh] Termos MeSH secundário: Feminino Seres Humanos Masculino Meia-Idade Obesidade/sangue Período Pós-Prandial Resultado do Tratamento [Pt] Tipo de publicação: JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL [Nm] Nome de substância: 0 (Ghrelin); 0 (Plant Extracts); 9005-80-5 (Inulin) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170522 [Lr] Data última revisão: 170522 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161223 [St] Status: MEDLINE [do] DOI: 10.1089/jmf.2016.0116

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[PMID]: 27889478 [Au] Autor: Pérez-Pimienta JA; Vargas-Tah A; López-Ortega KM; Medina-López YN; Mendoza-Pérez JA; Avila S; Singh S; Simmons BA; Loaces I; Martinez A [Ad] Endereço: Department of Chemical Engineering, Universidad Autónoma de Nayarit, Tepic, Mexico. Electronic address: japerez@uan.edu.mx. [Ti] Título: Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production. [So] Source: Bioresour Technol;225:191-198, 2017 Feb. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48h), 12.1 and 12.7kg of ethanol were produced per 100kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications. [Mh] Termos MeSH primário: Agave Biocombustíveis Celulose Etanol Líquidos Iônicos/química [Mh] Termos MeSH secundário: Agave/química Agave/metabolismo Celulose/química Celulose/metabolismo Etanol/análise Etanol/metabolismo Fermentação Lignina/análise Lignina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biofuels); 0 (Ionic Liquids); 3K9958V90M (Ethanol); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); 9006-97-7 (bagasse) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170131 [Lr] Data última revisão: 170131 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161128 [St] Status: MEDLINE

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[PMID]: 27730409 [Au] Autor: Mellado-Mojica E; González de la Vara LE; López MG [Ad] Endereço: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato. Km. 9.6 Lib. Norte Carretera Irapuato-León, Apartado Postal 629, 36821, Irapuato, Gto, Mexico. [Ti] Título: Fructan active enzymes (FAZY) activities and biosynthesis of fructooligosaccharides in the vacuoles of Agave tequilana Weber Blue variety plants of different age. [So] Source: Planta;245(2):265-281, 2017 Feb. [Is] ISSN: 1432-2048 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: MAIN CONCLUSION: Biosynthesis of agave fructans occurs in mesontle vacuoles which showed fluctuations in FAZY activities and synthesized a diverse spectrum of fructooligosaccharide isomers. Agave tequilana Weber Blue variety is an important agronomic crop in Mexico. Fructan metabolism in A. tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure. Specific activities of vacuolar fructan active enzymes (FAZY) in A. tequilana plants of different age and the biosynthesis of fructooligosaccharides (FOSs) were analyzed in this work. Vacuoles from mesontle (stem) protoplasts were isolated and collected from 2- to 7-year-old plants. For the first time, agave fructans were identified in the vacuolar content by HPAEC-PAD. Several FAZY activities (1-SST, 6-SFT, 6G-FFT, 1-FFT, and FEH) with fluctuations according to the plant age were found in protein vacuolar extracts. Among vacuolar FAZY, 1-SST activities appeared in all plant developmental stages, as well as 1-FFT and FEH activities. The enzymes 6G-FFT and 6-SST showed only minimal activities. Lowest and highest FAZY activities were found in 2- and 6-year-old plants, respectively. Synthesized products (FOS) were analyzed by TLC and HPAEC-PAD. Vacuolar FAZYs yielded large FOS isomers diversity, being 7-year-old plants the ones that synthesized a greater variety of fructans with different DP, linkages, and molecular structures. Based on the above, we are proposing a model for the FAZY activities constituting the FOS biosynthetic pathways in Agave tequilana Weber Blue variety. [Mh] Termos MeSH primário: Agave/fisiologia Oligossacarídeos/biossíntese Proteínas de Plantas/metabolismo Vacúolos/metabolismo [Mh] Termos MeSH secundário: Agave/metabolismo Configuração de Carboidratos Metabolismo dos Carboidratos Frutanos/metabolismo Glicosídeo Hidrolases/metabolismo Hexosiltransferases/metabolismo Oligossacarídeos/análise Oligossacarídeos/química Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fructans); 0 (Oligosaccharides); 0 (Plant Proteins); 0 (fructooligosaccharide); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.- (sucrose - fructan 6-fructosyltransferase); EC 2.4.1.100 (1,2-beta-fructan 1(F)-fructosyltransferase); EC 2.4.1.9 (inulosucrase); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.65 (levanase) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 171005 [Lr] Data última revisão: 171005 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161013 [St] Status: MEDLINE [do] DOI: 10.1007/s00425-016-2602-7

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[PMID]: 27534411 [Au] Autor: Sánchez-Ramírez J; Martínez-Hernández JL; Segura-Ceniceros P; López G; Saade H; Medina-Morales MA; Ramos-González R; Aguilar CN; Ilyina A [Ad] Endereço: Nanobioscience Group and Food Research Department, Chemistry Faculty, Universidad Autónoma de Coahuila, Blvd. V. Carranza e Ing. J. Cárdenas, 25280, Saltillo, Coahuila, Mexico. [Ti] Título: Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis. [So] Source: Bioprocess Biosyst Eng;40(1):9-22, 2017 Jan. [Is] ISSN: 1615-7605 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: In the present study, Trichoderma reesei cellulase was covalently immobilized on chitosan-coated magnetic nanoparticles using glutaraldehyde as a coupling agent. The average diameter of magnetic nanoparticles before and after enzyme immobilization was about 8 and 10 nm, respectively. The immobilized enzyme retained about 37 % of its initial activity, and also showed better thermal and storage stability than free enzyme. Immobilized cellulase retained about 80 % of its activity after 15 cycles of carboxymethylcellulose hydrolysis and was easily separated with the application of an external magnetic field. However, in this reaction, K was increased eight times. The immobilized enzyme was able to hydrolyze lignocellulosic material from Agave atrovirens leaves with yield close to the amount detected with free enzyme and it was re-used in vegetal material conversion up to four cycles with 50 % of activity decrease. This provides an opportunity to reduce the enzyme consumption during lignocellulosic material saccharification for bioethanol production. [Mh] Termos MeSH primário: Agave/química Biomassa Celulases/química Quitosana/química Proteínas Fúngicas/química Lignina/química Nanopartículas de Magnetita/química Trichoderma/enzimologia [Mh] Termos MeSH secundário: Enzimas Imobilizadas/química Hidrólise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzymes, Immobilized); 0 (Fungal Proteins); 0 (Magnetite Nanoparticles); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); 9012-76-4 (Chitosan); EC 3.2.1.- (Cellulases) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170221 [Lr] Data última revisão: 170221 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160819 [St] Status: MEDLINE [do] DOI: 10.1007/s00449-016-1670-1

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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