[PMID]: | 27730409 |
[Au] Autor: | Mellado-Mojica E; González de la Vara LE; López MG |
[Ad] Endereço: | Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato. Km. 9.6 Lib. Norte Carretera Irapuato-León, Apartado Postal 629, 36821, Irapuato, Gto, Mexico. |
[Ti] Título: | Fructan active enzymes (FAZY) activities and biosynthesis of fructooligosaccharides in the vacuoles of Agave tequilana Weber Blue variety plants of different age. |
[So] Source: | Planta;245(2):265-281, 2017 Feb. |
[Is] ISSN: | 1432-2048 |
[Cp] País de publicação: | Germany |
[La] Idioma: | eng |
[Ab] Resumo: | MAIN CONCLUSION: Biosynthesis of agave fructans occurs in mesontle vacuoles which showed fluctuations in FAZY activities and synthesized a diverse spectrum of fructooligosaccharide isomers. Agave tequilana Weber Blue variety is an important agronomic crop in Mexico. Fructan metabolism in A. tequilana exhibits changes in fructan content, type, degree of polymerization (DP), and molecular structure. Specific activities of vacuolar fructan active enzymes (FAZY) in A. tequilana plants of different age and the biosynthesis of fructooligosaccharides (FOSs) were analyzed in this work. Vacuoles from mesontle (stem) protoplasts were isolated and collected from 2- to 7-year-old plants. For the first time, agave fructans were identified in the vacuolar content by HPAEC-PAD. Several FAZY activities (1-SST, 6-SFT, 6G-FFT, 1-FFT, and FEH) with fluctuations according to the plant age were found in protein vacuolar extracts. Among vacuolar FAZY, 1-SST activities appeared in all plant developmental stages, as well as 1-FFT and FEH activities. The enzymes 6G-FFT and 6-SST showed only minimal activities. Lowest and highest FAZY activities were found in 2- and 6-year-old plants, respectively. Synthesized products (FOS) were analyzed by TLC and HPAEC-PAD. Vacuolar FAZYs yielded large FOS isomers diversity, being 7-year-old plants the ones that synthesized a greater variety of fructans with different DP, linkages, and molecular structures. Based on the above, we are proposing a model for the FAZY activities constituting the FOS biosynthetic pathways in Agave tequilana Weber Blue variety. |
[Mh] Termos MeSH primário: |
Agave/fisiologia Oligossacarídeos/biossíntese Proteínas de Plantas/metabolismo Vacúolos/metabolismo
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[Mh] Termos MeSH secundário: |
Agave/metabolismo Configuração de Carboidratos Metabolismo dos Carboidratos Frutanos/metabolismo Glicosídeo Hidrolases/metabolismo Hexosiltransferases/metabolismo Oligossacarídeos/análise Oligossacarídeos/química Fatores de Tempo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Fructans); 0 (Oligosaccharides); 0 (Plant Proteins); 0 (fructooligosaccharide); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.- (sucrose - fructan 6-fructosyltransferase); EC 2.4.1.100 (1,2-beta-fructan 1(F)-fructosyltransferase); EC 2.4.1.9 (inulosucrase); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.65 (levanase) |
[Em] Mês de entrada: | 1704 |
[Cu] Atualização por classe: | 171005 |
[Lr] Data última revisão:
| 171005 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161013 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1007/s00425-016-2602-7 |
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