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[PMID]:27922695
[Au] Autor:Kim JE; Koh EK; Song SH; Sung JE; Lee HA; Lee HG; Choi YW; Hwang DY
[Ad] Endereço:Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam­do 627­706, Republic of Korea.
[Ti] Título:Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon.
[So] Source:Mol Med Rep;15(1):431-441, 2017 Jan.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The laxative effects of aqueous extract of Liriope platyphylla (AEtLP) on loperamide (Lop)­induced constipation have been reported; however, the key compounds and the mechanism underlying these effects remain unclear. Therefore, the laxative effects of five candidates derived from L. platyphylla: Diosgenin (DG), 5-hydroxymethylfurfural (5-HMF), adenosine (AD), hydroxypropyl cellulose (HPC) and uridine (UD) were investigated by examining the alteration of G protein α (Gα) expression, protein kinase C (PKC) phosphorylation and inositol triphosphate (IP3) concentration levels in the 5-hydroxytryptamine (5­HT; serotonin) receptor signaling pathway. Primary rat intestine smooth muscle cells (pRISMCs), intestinal epithelial cells (IEC)­18 and B35 cells were cotreated with Lop and the five compounds in order to screen the candidates. AEtLP, prucalopride (PCP) and bisacodyl (BS) served as positive controls. In pRISMCs, Gα expression levels were recovered in the majority of candidate­treated groups, whereas PKC phosphorylation recovery was observed only in the DG, 5­HMF and AD treatment groups. In IEC­18 cells, the AD treatment group mimicked the effects of PCP on PKC phosphorylation levels, whereas the DG, 5­HMF, HPC and UD treatment groups mimicked the effects of AEtLP and BS. In B35 cells, a greater upregulation of PKC phosphorylation levels were observed in the UD treatment group compared with the PCP and BS treatment groups, whereas DG, 5­HMF and AD treatment reduced the PKC phosphorylation levels to a greater extent than AEtLP treatment. However, effects similar to AEtLP, PCP and BS on Gα expression levels were not detected in any treatment groups in IEC­18 and B35 cells. Furthermore, the level of IP3 was enhanced only in pRISMCs, in which all five candidates were effective, while the greatest concentration was observed in the UD treatment group. In conclusion, the results of the present study suggest that UD may be considered the compound with the greatest laxative activity, which may regulate the 5­HT receptor signaling pathway.
[Mh] Termos MeSH primário: Colo Transverso/efeitos dos fármacos
Laxantes/química
Laxantes/farmacologia
Liriope (Planta)/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Receptores de Serotonina/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Colo Transverso/citologia
Colo Transverso/metabolismo
Constipação Intestinal/tratamento farmacológico
Constipação Intestinal/metabolismo
Feminino
Laxantes/isolamento & purificação
Extratos Vegetais/isolamento & purificação
Ratos Sprague-Dawley
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Laxatives); 0 (Plant Extracts); 0 (Receptors, Serotonin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5983


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[PMID]:27627915
[Au] Autor:Kim MJ; Yoo YC; Sung NY; Lee J; Park SR; Shon EJ; Lee BD; Kim MR
[Ad] Endereço:* Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
[Ti] Título:Anti-Inflammatory Effects of Liriope platyphylla in LPS-Stimulated Macrophages and Endotoxemic Mice.
[So] Source:Am J Chin Med;44(6):1127-1143, 2016.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:In the present study, the anti-inflammatory and antisepticemic activities of a water extract of Liriope platyphylla (LP) were investigated. We first estimated the scavenging activity of DPPH and the hydroxyl radical and total phenolic contents of LP. Results indicated that LP, a rich source of phenolic compounds, showed a remarkable radical scavenging capacity. A MTT assay showed that LP treatment did not affect the toxicity against the RAW 264.7 macrophage cells, up to the concentration of 500[Formula: see text][Formula: see text]g/mL. Treatment of LP significantly attenuated the production of inflammatory mediators, such as nitric oxide (NO), interleukin-6 (IL-6), tumor-necrosis factor (TNF)-[Formula: see text] and prostaglandin (PG)E in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages cells. Moreover, LP contributed to the down-regulation of inducible NO synthase (iNOS) and TNF-[Formula: see text] mRNA expression, as well as cyclooxygenase-2 (COX-2) protein expression. A western blotting assay further showed that LP inhibited activation of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-[Formula: see text]B. In an animal experiment using an LPS-induced septicemia model in C57BL/6 mice, oral administration of LP (40[Formula: see text]mg/kg body weight) markedly reduced the level of TNF-[Formula: see text] and IL-6 in serum and protected against LPS-induced lethal shock in mice. Taken together, the results of treatments of LP on inhibited LPS-induced inflammatory responses in both in vitro and in vivo models and indicate it may be a promising neutraceutical or medicinal agent to prevent or cure inflammation-related disease.
[Mh] Termos MeSH primário: Antibacterianos
Anti-Inflamatórios
Endotoxemia/tratamento farmacológico
Lipopolissacarídeos/efeitos adversos
Liriope (Planta)/química
Macrófagos/efeitos dos fármacos
Fitoterapia
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Modelos Animais de Doenças
Endotoxemia/metabolismo
Depuradores de Radicais Livres/análise
Depuradores de Radicais Livres/isolamento & purificação
Mediadores da Inflamação/metabolismo
Interleucina-6/metabolismo
Macrófagos/metabolismo
Camundongos
Fenóis/análise
Fenóis/isolamento & purificação
Extratos Vegetais/administração & dosagem
Extratos Vegetais/isolamento & purificação
Células RAW 264.7
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Free Radical Scavengers); 0 (Inflammation Mediators); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Phenols); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


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[PMID]:27079642
[Au] Autor:Li H; Sun L; de Carvalho EL; Li X; Lv X; Khan GJ; Semukunzi H; Yuan S; Lin S
[Ad] Endereço:Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:DT-13, a saponin monomer of dwarf lilyturf tuber, induces autophagy and potentiates anti-cancer effect of nutrient deprivation.
[So] Source:Eur J Pharmacol;781:164-72, 2016 Jun 15.
[Is] ISSN:1879-0712
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Metabolic stress induces autophagy as a protective mechanism in tumorigenesis and development. Conversely, excessive autophagy in nutrient-deprived cancer cells would be beneficial for cancer therapy. DT-13, the saponin monomer 13 of the Dwarf lilyturf tuber, inhibited tumor metastasis and angiogenesis in previous studies. However, there is scarcity of data regarding the effect of DT-13 on autophagy process. Here, we demonstrated that DT-13 induced autophagy in human cancer cell lines and caused significant cell apoptosis under nutrient starvation. We firstly showed that DT-13 increased the accumulation of GFP-LC3 puncta and induced the expression of LC3-II in a dose- and time-dependent manner. DT-13 also upregulated the expression of Beclin-1, Atg-3 and Atg-7, and induced autophagic flux in human gastric cancer BGC-823 cells. We next found that low-toxic concentrations of DT-13 significantly induced apoptosis under nutrient deprivation. We finally demonstrated that the PI3K/Akt/mTOR signal pathway was involved in the cytotoxic effect of DT-13. Our data indicated that DT-13 was a novel autophagy inducer and might be considered in future treatment of cancer.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Liriope (Planta)/química
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Autofagossomos/efeitos dos fármacos
Autofagossomos/metabolismo
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Seres Humanos
Proteínas Associadas aos Microtúbulos/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Saponinas/química
Transdução de Sinais/efeitos dos fármacos
Serina-Treonina Quinases TOR/metabolismo
Fatores de Tempo
Regulação para Cima/efeitos dos fármacos
Vacúolos/efeitos dos fármacos
Vacúolos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MAP1LC3A protein, human); 0 (Microtubule-Associated Proteins); 0 (Saponins); 0 (ruscogenin-1-O-(glucopyranosyl-(1-2))(xylopyranosyl-(1-3))fucopyranoside); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160416
[St] Status:MEDLINE


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[PMID]:26969403
[Au] Autor:Kim HJ; Park SY; Kim DG; Park SH; Lee H; Hwang DY; Jung MH; Ha KT; Kim BJ
[Ad] Endereço:Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 50612, Republic of Korea; Healthy Aging Korean Medical Research Center, Pusan National University School of Korean Medicine, Yangsan 50612, Republic of Korea.
[Ti] Título:Effects of the roots of Liriope Platyphylla Wang Et tang on gastrointestinal motility function.
[So] Source:J Ethnopharmacol;184:144-53, 2016 May 26.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Liriope platyphylla Wang et Tang continues to be used in Korea as a traditional medicine for the treatment of gastrointestinal (GI) disorders related to constipation and abnormal GI motility. AIM OF THE STUDY: Because GI disorders, especially GI motility dysfunctions, are major lifelong problems, the authors investigated the effects of a water extract of the roots of L. platyphylla Wang et Tang (LPE) on the pacemaker potentials (PPTs) of interstitial cells of Cajal (ICCs) and on GI motility in male ICR mice. MATERIALS AND METHODS: Enzymatic digestions were used to dissociate ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record PPTs generated by cultured ICCs in vitro. In vivo effects of LPE on GI motility were investigated by measuring intestinal transit rates (ITRs) of Evans blue in normal mice and in acetic acid (AA) and streptozotocin (STZ)-induced diabetic mouse models of GI motility dysfunction. RESULTS: LPE dose-dependently depolarized PPTs in ICCs. Pretreatment with methoctramine (a muscarinic M2 receptor antagonist) did not block LPE-induced PPT depolarization. However, pretreatment with 4-DAMP (a muscarinic M3 receptor antagonist) blocked LPE-induced PPT depolarization. In addition, treatment with LY294002 (a phosphoinositide 3-kinase (PI3K) inhibitor) also blocked LPE-induced PPT depolarization. Intracellular GDPßS inhibited LPE-induced PPT depolarization, and LPE-induced PPT depolarization was found to occur in a phospholipase C (PLC)- and a protein kinase C (PKC)-dependent manner. Pretreatment with Ca(2+)free solution or thapsigargin (a Ca(2+)-ATPase inhibitor in endoplasmic reticulum) abolished PPTs, and under these conditions, LPE did not depolarize ICC PPTs. In normal mice, ITRs were significantly and dose-dependently increased by LPE (0.01-1g/kg administered intragastrically (i.g.)). In addition, LPE (i.g.) significantly recovered GI motility dysfunctions in both animal models. CONCLUSION: LPE dose-dependently depolarizes ICC PPTs through M3 receptors via external and internal Ca(2+)regulation and via G protein-, PI3K-, PLC- and PKC- dependent pathways in vitro. Also, in vivo, LPE increased ITRs in treatment naïve mice and our two mouse models of GI dysfunction. Therefore, this study shows that LPE offers a basis for the development of a prokinetic agent that prevents or alleviates GI motility dysfunctions.
[Mh] Termos MeSH primário: Motilidade Gastrointestinal/efeitos dos fármacos
Células Intersticiais de Cajal/efeitos dos fármacos
Liriope (Planta)
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Ácido Acético
Animais
Células Cultivadas
Diabetes Mellitus Experimental
Proteínas de Ligação ao GTP/fisiologia
Células Intersticiais de Cajal/fisiologia
Intestino Delgado/citologia
Intestino Delgado/fisiologia
Masculino
Camundongos Endogâmicos ICR
Fosfatidilinositol 3-Quinases/fisiologia
Raízes de Plantas
Proteína Quinase C/fisiologia
Fosfolipases Tipo C/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.13 (Protein Kinase C); EC 3.1.4.- (Type C Phospholipases); EC 3.6.1.- (GTP-Binding Proteins); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170106
[Lr] Data última revisão:
170106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE


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[PMID]:26411727
[Au] Autor:Mao C; Xie H; Chen S; Valverde BE; Qiang S
[Ad] Endereço:Weed Research Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
[Ti] Título:Multiple mechanism confers natural tolerance of three lilyturf species to glyphosate.
[So] Source:Planta;243(2):321-35, 2016 Feb.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: A combination of unique EPSPS structure and increased gene copy number and expression contribute to natural glyphosate tolerance in three lilyturf species. A few plants are naturally tolerant to glyphosate, the most widely used non-selective herbicide worldwide. Here, the basis for natural tolerance to glyphosate in three lilyturf species, Ophiopogon japonicus (OJ), Liriope spicata (LS), and Liriope platyphylla (LP), is characterized. These species tolerate glyphosate at about five times the commercially recommended field dose. They share three unique amino acids in their 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that affect glyphosate binding. These correspond to Asp71Met, Ala112Ile, and Val201Met amino acid variations compared to 231 other published plant EPSPS amino acid sequences. There was also a common deletion at 91 of a highly conserved glutamic acid. Glyphosate-treated lilyturf plants accumulated little shikimic acid but had significantly higher levels of EPSPS mRNA than initially expressed in the control. The IC50 of LsEPSPS was 14.0 µM compared to the 5.1 µM of Arabidopsis thaliana. The higher K m and K i values of LsEPSPS kinetics showed that LsEPSPS had lower substrate binding affinity to glyphosate. Overexpression of LsEPSPS in the recombinant E. coli BL21 (DE3) strain enhanced its tolerance to glyphosate. Both OJ and LS had two copies of the EPSPS gene, while LP had three copies. Therefore, a combination of unique EPSPS structure and increased gene copy number and expression contribute to natural glyphosate tolerance in the three lilyturf species.
[Mh] Termos MeSH primário: 3-Fosfoshikimato 1-Carboxiviniltransferase/química
Glicina/análogos & derivados
Liriope (Planta)/enzimologia
Ophiopogon/enzimologia
Proteínas de Plantas/química
[Mh] Termos MeSH secundário: 3-Fosfoshikimato 1-Carboxiviniltransferase/metabolismo
Sequência de Aminoácidos
Substituição de Aminoácidos
Sítios de Ligação
Clonagem Molecular
Glicina/farmacologia
Resistência a Herbicidas/genética
Liriope (Planta)/efeitos dos fármacos
Liriope (Planta)/genética
Modelos Moleculares
Dados de Sequência Molecular
Ophiopogon/efeitos dos fármacos
Ophiopogon/genética
Proteínas de Plantas/metabolismo
Estrutura Terciária de Proteína
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Análise de Sequência de Proteína
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Messenger); 4632WW1X5A (glyphosate); EC 2.5.1.19 (3-Phosphoshikimate 1-Carboxyvinyltransferase); TE7660XO1C (Glycine)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150929
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-015-2408-z


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[PMID]:26151867
[Au] Autor:Kim JE; Park SH; Kwak MH; Go J; Koh EK; Song SH; Sung JE; Lee HS; Hong JT; Hwang DY
[Ad] Endereço:Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, Korea.
[Ti] Título:Characterization of Changes in Global Genes Expression in the Distal Colon of Loperamide-Induced Constipation SD Rats in Response to the Laxative Effects of Liriope platyphylla.
[So] Source:PLoS One;10(7):e0129664, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To characterize the changes in global gene expression in the distal colon of constipated SD rats in response to the laxative effects of aqueous extracts of Liriope platyphylla (AEtLP), including isoflavone, saponin, oligosaccharide, succinic acid and hydroxyproline, the total RNA extracted from the distal colon of AEtLP-treated constipation rats was hybridized to oligonucleotide microarrays. The AEtLP treated rats showed an increase in the number of stools, mucosa thickness, flat luminal surface thickness, mucin secretion, and crypt number. Overall, compared to the controls, 581 genes were up-regulated and 216 genes were down-regulated by the constipation induced by loperamide in the constipated rats. After the AEtLP treatment, 67 genes were up-regulated and 421 genes were down-regulated. Among the transcripts up-regulated by constipation, 89 were significantly down-regulated and 22 were recovered to the normal levels by the AEtLP treatment. The major genes in the down-regulated categories included Slc9a5, klk10, Fgf15, and Alpi, whereas the major genes in the recovered categories were Cyp2b2, Ace, G6pc, and Setbp1. On the other hand, after the AEtLP treatment, ten of these genes down-regulated by constipation were up-regulated significantly and five were recovered to the normal levels. The major genes in the up-regulated categories included Serpina3n, Lcn2 and Slc5a8, whereas the major genes in the recovered categories were Tmem45a, Rerg and Rgc32. These results indicate that several gene functional groups and individual genes as constipation biomarkers respond to an AEtLP treatment in constipated model rats.
[Mh] Termos MeSH primário: Colo/metabolismo
Constipação Intestinal/tratamento farmacológico
Regulação para Baixo/efeitos dos fármacos
Laxantes/uso terapêutico
Liriope (Planta)/química
Extratos Vegetais/uso terapêutico
Regulação para Cima/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Colo/patologia
Constipação Intestinal/induzido quimicamente
Quinase 2 de Receptor Acoplado a Proteína G
Liriope (Planta)/metabolismo
Loperamida/toxicidade
Análise de Sequência com Séries de Oligonucleotídeos
Extratos Vegetais/química
Raízes de Plantas/química
Raízes de Plantas/metabolismo
Ratos
Ratos Sprague-Dawley
Receptores Proteína Tirosina Quinases/genética
Receptores Proteína Tirosina Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Laxatives); 0 (Plant Extracts); 6X9OC3H4II (Loperamide); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.11.15 (muscarinic receptor kinase); EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150708
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0129664


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[PMID]:26126769
[Au] Autor:Ito M; Sato-Masumoto N; Kobayashi F; Matsumura K
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan, shoyaku.shigen@gmail.com.
[Ti] Título:Distinguishing Ophiopogon and Liriope tubers based on DNA sequences.
[So] Source:J Nat Med;69(4):555-64, 2015 Oct.
[Is] ISSN:1861-0293
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Ophiopogon japonicus is a herbaceous perennial plant in Liliaceae, and its tubers are used in traditional Japanese medicine as Bakumondo, prescribed for treating cough, sputum, and thirst. Liriope is a genus of ornamental plants related to Ophiopogon, and its tubers are used in folk medicine as well. Although tubers from both genera are traded in Korean and Chinese markets, only O. japonicus is defined as the plant of origin for Bakumondo in the Japanese Pharmacopoeia [1], and Liriope tubers cannot legally be used as Bakumondo in Japan. Ophiopogon plants can be distinguished clearly from Liriope by their fruit color and by the morphological characteristics of their flowers. However, the tubers of both species are greatly similar, making it very difficult to differentiate the two genera by the appearance of their tubers. We, therefore, investigated the most appropriate DNA regions to use for practical and accurate identification of Ophiopogon and Liriope tubers. The sequence of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) was found to be suitable for discriminating Ophiopogon and Liriope tubers. The identification procedure was simplified using restriction enzyme digestion of the amplified rbcL fragment. The detection limit for Liriope contamination was estimated by performing the procedure using mixed samples of powdered Ophiopogon and Liriope tubers.
[Mh] Termos MeSH primário: Sequência de Bases/fisiologia
DNA/metabolismo
Liriope (Planta)/química
Ophiopogon/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150702
[St] Status:MEDLINE
[do] DOI:10.1007/s11418-015-0924-6


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[PMID]:26073343
[Au] Autor:Li YW; Qi J; Zhang YY; Huang Z; Kou JP; Zhou SP; Zhang Y; Yu BY
[Ad] Endereço:State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China.
[Ti] Título:Novel cytotoxic steroidal glycosides from the roots of Liriope muscari.
[So] Source:Chin J Nat Med;13(6):461-6, 2015 Jun.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The present study was designed to investigate the chemical constituents and bioactivities of the roots of Liriope muscari (Decne.) L.H. Bailey. The compounds were isolated through various chromatography techniques, including silica gel, Sephadex LH-20, and semi-preparative HPLC. The structures were elucidated by infrared (IR), mass spectrometric (MS), 1D- and 2D-NMR analyses in comparison with reference data. In addition, the cytotoxicity of these compounds against human breast cancer MDA-MB-435 cells was evaluated by the MTT assay. Two new steroidal glycosides, 25(R, S)-ruscogenin-1-O-[ß-D-fucopyranosyl (1→2)]-[ß-D-xylopyranosyl(1→3)]ß-D-glucopyranoside (Liriopem I, 1) and 25(R, S)- ruscogenin-1-O-[ß-D-fucopyranosyl (1→2)]-[ß-D-xylopyranosyl(1→4)]-ß-D-fucopyranoside (Liriopem II, 2 and two known compounds LM-S6 (3) and DT-13 (4) were isolated and identified. Liriopem I(1), liriopem II(2) and DT-13 (4) showed remarkable cytotoxicity with IC50 values being (0.58 ± 0.08), (0.05 ± 0.10), and (0.15 ± 0.09) µg·mL(-1), respectively. In summary, compounds 1 and 2 identified in the present study exerted cytotoxicity against breast cancer cells, providing a basis for future development of these compounds as novel anticancer agents.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/toxicidade
Glicosídeos/toxicidade
Liriope (Planta)/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Glicosídeos/química
Glicosídeos/isolamento & purificação
Seres Humanos
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Glycosides)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150615
[Lr] Data última revisão:
150615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150616
[St] Status:MEDLINE


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[PMID]:25967662
[Au] Autor:Kim KS; Cho DH; Yang HJ; Choi EK; Shin MH; Kim KH; Ahn KS; Ha IJ; Na YC; Um JY; Chung WS; Jung HJ; Jung SK; Jang HJ
[Ad] Endereço:College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
[Ti] Título:Effects of the inhaled treatment of liriope radix on an asthmatic mouse model.
[So] Source:Am J Chin Med;43(3):425-41, 2015.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:As a treatment for allergic asthma, inhaled treatments such as bronchodilators that contain ß2-agonists have an immediate effect, which attenuates airway obstructions and decreases airway hypersensitivity. However, bronchodilators only perform on a one off basis, but not consistently. Asthma is defined as a chronic inflammatory disease of the airways accompanying the overproduction of mucus, airway wall remodeling, bronchial hyperreactivity and airway obstruction. Liriope platyphylla radix extract (LPP), a traditional Korean medicine, has been thoroughly studied and found to be an effective anti-inflammatory medicine. Here, we demonstrate that an inhaled treatment of LPP can attenuate airway hyperresponsiveness (AHR) in an ovalbumin-induced asthmatic mouse model, compared to the saline-treated group (p < 0.01). Moreover, LPP decreases inflammatory cytokine levels, such as eotaxin (p < 0.05), IL-5 (p < 0.05), IL-13 (p < 0.001), RANTES (p < 0.01), and TNF-α (p < 0.05) in the bronchoalveolar lavage (BAL) fluid of asthmatic mice. A histopathological study was carried out to determine the effects of LPP inhalation on mice lung tissue. We performed UPLC/ESI-QTOF-MS, LC/MS, and GC/MS analyses to analyze the chemical constituents of LPP, finding that these are ophiopogonin D, spicatoside A, spicatoside B, benzyl alcohol, and 5-hydroxymethylfurfural. This study demonstrates the effect of an inhaled LPP treatment both on airway AHR and on the inflammatory response in an asthmatic mouse model. Hence, LPP holds significant promise as a nasal inhalant for the treatment of asthmatic airway disease.
[Mh] Termos MeSH primário: Asma/tratamento farmacológico
Liriope (Planta)
Medicina Tradicional Coreana
Fitoterapia
Extratos Vegetais/administração & dosagem
Hipersensibilidade Respiratória/tratamento farmacológico
[Mh] Termos MeSH secundário: Administração por Inalação
Animais
Asma/induzido quimicamente
Asma/metabolismo
Líquido da Lavagem Broncoalveolar/química
Citocinas/metabolismo
Depressão Química
Modelos Animais de Doenças
Feminino
Mediadores da Inflamação/metabolismo
Camundongos Endogâmicos BALB C
Ovalbumina
Extratos Vegetais/farmacologia
Hipersensibilidade Respiratória/induzido quimicamente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators); 0 (Plant Extracts); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150513
[Lr] Data última revisão:
150513
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X15500275


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[PMID]:25913925
[Au] Autor:Tsai YC; Hsu CC; El-Shazly M; Chiang SY; Wu CC; Wu CC; Lai WC; Yen MH; Wu YC; Chang FR
[Ad] Endereço:Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yuchi0713@gmail.com.
[Ti] Título:Phytochemicals and Estrogen-Receptor Agonists from the Aerial Parts of Liriope platyphylla.
[So] Source:Molecules;20(4):6844-55, 2015 Apr 16.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:One new benzofuran, (2R)-(2',4'-dihydroxybenzyl)-6,7-methylenedioxy-2,3-dihydrobenzofuran (1), one new phenylisocoumarin, 3-(2'-hydroxyphenyl)-6,8-dihydroxy-7-methoxy-isocoumarin (2), and one new benzofuroisocoumarin, platyphyllarin C (3), were isolated from the ethanolic extract of Liriope platyphylla aerial parts, along with seventeen known compounds. The structures of the isolates were established by spectroscopic analysis and comparison with the literature data. The results indicated that structures 1-3 are uncommon in Nature. Benzofuroisocoumarin 4, flavonoids 9, 10, and 13-15, and homoisoflavonoids 19 and 20 exhibited significant binding activity to estrogen-receptor α and/or ß as demonstrated by the SEAP reporter assay system in an MCF-7 cell-line.
[Mh] Termos MeSH primário: Estrogênios/farmacologia
Liriope (Planta)/química
Compostos Fitoquímicos/química
Componentes Aéreos da Planta/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Cromanos/química
Cromanos/isolamento & purificação
Cromanos/farmacologia
Receptor alfa de Estrogênio/metabolismo
Receptor beta de Estrogênio/metabolismo
Estrogênios/química
Estrogênios/isolamento & purificação
Seres Humanos
Isocumarinas/química
Isocumarinas/isolamento & purificação
Isocumarinas/farmacologia
Células MCF-7
Estrutura Molecular
Compostos Fitoquímicos/isolamento & purificação
Compostos Fitoquímicos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromans); 0 (Estrogen Receptor alpha); 0 (Estrogen Receptor beta); 0 (Estrogens); 0 (Isocoumarins); 0 (Phytochemicals); 0 (Plant Extracts)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150428
[Lr] Data última revisão:
150428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150428
[St] Status:MEDLINE
[do] DOI:10.3390/molecules20046844



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