Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.100.060.600 [Categoria DeCS]
Referências encontradas : 149 [refinar]
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[PMID]:28598312
[Au] Autor:Mingma R; Duangmal K; Omura S; Takahashi Y; Matsumoto A
[Ad] Endereço:2​Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand 1​Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
[Ti] Título:Three novel species of the genus Kibdelosporangium; Kibdelosporangium kanagawaense sp. nov., Kibdelosporangiumrhizosphaerae sp. nov. and Kibdelosporangium rhizovicinum sp. nov.
[So] Source:Int J Syst Evol Microbiol;67(6):1758-1765, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The taxonomic positions of three actinomycete isolates, K08-0175T, K10-0543T and K12-0791T, which were isolated from plant root and rhizospheric soil samples were subjected to a polyphasic taxonomic study. On the basis of the results of phylogenetic analysis and morphological and chemotaxonomic characteristics, these three strains were classified as representing members of the genus Kibdelosporangium. These strains were observed to produce both long chains of rod-shaped spores and sporangium-like structures with well-defined walls on aerial hyphae. Phylogenetic position, DNA-DNA hybridization and comparison of the phylogenetically closest relatives revealed that these three strains were clearly distinguishable from each other and from their closest phylogenetic relatives. Therefore, three novel species are proposed as Kibdelosporangium kanagawaense sp. nov. [type strain K08-0175T (=NBRC 112388T=TBRC 6786T)], Kibdelosporangiumrhizosphaerae sp. nov. [type strain K10-0543T (=NBRC 112389T=TBRC 6787T)] and Kibdelosporangium rhizovicinum sp. nov. [type strain K12-0791T (=NBRC 112390T=TBRC 6788T)].
[Mh] Termos MeSH primário: Actinomycetales/classificação
Ophiopogon/microbiologia
Filogenia
Raízes de Plantas/microbiologia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Actinomycetales/genética
Actinomycetales/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Japão
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Rizosfera
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001861


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[PMID]:28598242
[Au] Autor:Zhang YL; Xi MZ; Choi YB; Lee BH
[Ad] Endereço:Department of Food and Nutrition, Chung-Ang University , Anseong-si, Gyeonggi-do, Korea.
[Ti] Título:Antithrombotic Effect of Fermented Ophiopogon japonicus in Thrombosis-Induced Rat Models.
[So] Source:J Med Food;20(7):637-645, 2017 Jul.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the antithrombotic and thrombolytic ability of second fermented extract of Ophiopogon japonicus (FEOJ) was verified in thrombosis-induced rats. Thrombosis was induced by oral administration of 2% carrageenan for 4 weeks. Five experimental groups (n = 9/group) involved in the study were control group, thrombosis group, low-dose FEOJ group (2 mL/kg, low-dose Ophiopogon japonicus [LOJ]), middle-dose FEOJ group (6 mL/kg, medium-dose Ophiopogon japonicus [MOJ]), and high-dose FEOJ group (12 mL/kg, high-dose Ophiopogon japonicus [HOJ]). The clotting time (CT), bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FBG) were assessed in blood samples, and histological studies were performed on liver and lung tissues. The results demonstrated delayed CT only in MOJ and HOJ groups and delayed BT in all FEOJ groups compared with those in thrombosis and control groups (P < .05). Similarly, APTT was significantly delayed only in MOJ and HOJ groups, and PT was significantly delayed in all FEOJ groups, compared with those in control and thrombosis groups (P < .05). Although concentrations of FBG were similar in control, thrombosis, and LOJ groups, the tendency for decreased concentration of FBG (statistically nonsignificant) in MOJ and HOJ groups has been observed. Histological examination of livers and lungs revealed that thrombosis was partially improved in FEOJ group compared with the thrombosis group. In conclusion, CT, BT, PT, and APTT were prolonged in FEOJ group more than in control and thrombosis groups, thereby, depicting antithrombotic and thrombolytic effects. However, concentration-dependent effects of FEOJ were more prominent in MOJ and HOJ groups than in the LOJ group.
[Mh] Termos MeSH primário: Anticoagulantes/administração & dosagem
Ophiopogon/química
Extratos Vegetais/administração & dosagem
Trombose/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Anticoagulantes/isolamento & purificação
Anticoagulantes/metabolismo
Tempo de Sangramento
Coagulação Sanguínea/efeitos dos fármacos
Carragenina/efeitos adversos
Fermentação
Seres Humanos
Lactobacillaceae/metabolismo
Masculino
Ophiopogon/microbiologia
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/metabolismo
Tempo de Protrombina
Ratos
Ratos Sprague-Dawley
Trombose/sangue
Trombose/induzido quimicamente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (Plant Extracts); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.3872


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[PMID]:28284426
[Au] Autor:Liu CH; Qi J; Zhou DZ; Ju AC; Yu BY
[Ad] Endereço:Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China; Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.
[Ti] Título:Influence of ultrafiltration membrane on ophiopogonins and homoisoflavonoids in Ophiopogon japonicus as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry.
[So] Source:Chin J Nat Med;15(2):121-141, 2017 Feb.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus (L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off (MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone (PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate (%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Isoflavonas/análise
Ophiopogon/química
Extratos Vegetais/química
Polímeros
Saponinas/análise
Sulfonas
[Mh] Termos MeSH secundário: Cromatografia Líquida/métodos
Medicamentos de Ervas Chinesas
Estrutura Molecular
Peso Molecular
Espectrometria de Massas por Ionização por Electrospray/métodos
Ultrafiltração/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Isoflavones); 0 (Plant Extracts); 0 (Polymers); 0 (Saponins); 0 (Sulfones); 25667-42-9 (polyether sulfone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  4 / 149 MEDLINE  
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[PMID]:28196345
[Au] Autor:Zhao M; Xu WF; Shen HY; Shen PQ; Zhang J; Wang DD; Xu H; Wang H; Yan TT; Wang L; Hao HP; Wang GJ; Cao LJ
[Ad] Endereço:State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Tongjiaxiang 24, Nanjing, 21009, China.
[Ti] Título:Comparison of bioactive components and pharmacological activities of ophiopogon japonicas extracts from different geographical origins.
[So] Source:J Pharm Biomed Anal;138:134-141, 2017 May 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ophiopogon japonicus (Linn. f.) Ker-Gawl (O. japonicas), mainly cultivated in Sichuan and Zhejiang province in China, has different bioactive components and therefore their pharmacological activities. To explain the different clinical efficacy of O. japonicas derived preparations, herein we report differences of pharmacological activities between Sichuan and Zhejiang O. japonicas and behind them the exact differences of bioactive components. Based on a LC/MS-IT-TOF method, the differences of bioactive components between Sichuan and Zhejiang O. japonicas extracts were analyzed and respective characteristic components were picked out. We determined 39 ophiopogonones and 71 ophiopogonins compounds in Sichuan and Zhejiang O. japonicas extracts and found the contents of these compositions have several times difference. Evidenced by experimental data of pharmacological activities in inhibiting cardiomyocyte damage induced by H O , mouse macrophage cell inflammation induced by lipopolysaccharide and cytotoxicity in vitro, Zhejiang O. japonicas extract had a stronger antioxidant and anti-inflammatory capacity than Sichuan O. japonicas extract, and the two O. japonicas extracts exhibited selective cytotoxicity on different cancer cell lines in vitro. These data shed light on the links between bioactive components and pharmacological activities of O. japonicas derived preparations. Thus, geographical origin of O. japonicas should be considered to be a key factor in efficacy studies and further clinical application.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Ophiopogon/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Células A549
Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Antioxidantes/química
Antioxidantes/farmacologia
Linhagem Celular
Linhagem Celular Tumoral
China
Células HCT116
Células HT29
Células Hep G2
Seres Humanos
Células MCF-7
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE


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[PMID]:28139298
[Au] Autor:Wang L; Jiang XL; Zhang WM; Li F; Khan AA; Liu X; Yu K; Wang MK
[Ad] Endereço:School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of S
[Ti] Título:Homo-aro-cholestane, furostane and spirostane saponins from the tubers of Ophiopogon japonicus.
[So] Source:Phytochemistry;136:125-132, 2017 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation of the tubers of Ophiopogon japonicus led to the isolation of five previously undescribed steroidal saponins, ophiojaponins A-E, together with twelve known ones. The structures of these isolated compounds were elucidated by detailed spectroscopic analyses and chemical methods. Ophiojaponins A-C are rare naturally occurring C steroidal glycosides possessing a homo-cholestane skeleton with an aromatized ring E. Ruscogenin 1-O-α-L-rhamnopyranosyl-(1 â†’ 2)-4-O-sulfo-ß-D-fucopyranosido-3-O-ß-D-glucopyranoside was isolated as single component and its full spectroscopic data was reported for the first time. The isolated steroidal saponins were evaluated for their cytotoxicities against two human tumor cell lines MG-63 and SNU387. Among them, five known spirostane-type glycosides showed cytotoxic activity against both MG-63 and SNU387 cell lines with IC values ranging from 0.76 to 27.0 µM.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Colestanos/isolamento & purificação
Medicamentos de Ervas Chinesas/isolamento & purificação
Ophiopogon/química
Tubérculos/química
Saponinas/isolamento & purificação
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Colestanos/química
Colestanos/farmacologia
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Estrutura Molecular
Saponinas/química
Saponinas/farmacologia
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Cholestanes); 0 (Drugs, Chinese Herbal); 0 (Saponins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE


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[PMID]:28056939
[Au] Autor:Zhao JW; Chen DS; Deng CS; Wang Q; Zhu W; Lin L
[Ad] Endereço:The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China.
[Ti] Título:Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas.
[So] Source:BMC Complement Altern Med;17(1):7, 2017 Jan 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ophiopogon japonicas (L.f) Ker-Gawl has been used as a traditional Chinese medicine to cure acute and chronic inflammation and cardiovascular diseases including thrombotic diseases for thousands of years. Previous phytochemical studies showed that O. japonicus contained compounds with anti-inflammatory activity. The aim of this study was to identify and isolate compounds with anti-inflammatory activity from the rhizome of O. japonicas. METHODS: Compounds were isolated by various column chromatography and their structures were identified in terms of nuclear magnetic resonance spectrum (NMR) and mass spectrum (MS). To measure the anti-inflammatory effects of thirteen compounds in LPS-induced RAW 264.7 macrophage cells, we used the following methods: cell viability assay, nitric oxide assay, enzyme-linked immunosorbent assay, quantitative real-time PCR analysis and western blotting analysis. RESULTS: One new and twelve known compounds (mainly homoisoflavonoids) were extracted from O. japonicas, in which 4'-O-Demethylophiopogonanone E (10) was considered as a new compound, additionally, compounds 4-O-(2-Hydroxy-1- hydroxymethylethyl)-dihydroconiferyl alcohol (2) and 5,7-dihydroxy-6-methyl-3-(2', 4'-dihydroxybenzyl) chroman-4-one (12) were isolated from the rhizome of O. japonicas for the first time. The isolated compounds Oleic acid (3), Palmitic acid (4), desmethylisoophiopogonone B [5,7-dihydroxy-3-(4'-hydroxybenzyl)-8- methyl- chromone] (5), 5,7-dihydroxy-6-methyl-3-(4'-hydroxybenzyl) chromone (7) and 10 significantly suppressed the production of NO in LPS-induced RAW 264.7 cells. Especially compound 10 showed the strongest effect against the production of the pro-inflammatory cytokine IL-1ß and IL-6 with the IC value of 32.5 ± 3.5 µg/mL and 13.4 ± 2.3 µg/mL, respectively. Further analysis elucidated that the anti-inflammatory activity of compound 10 might be exerted through inhibiting the phosphorylation of ERK1/2 and JNK in MAPK signaling pathways to decrease NO and pro-inflammatory cytokines production. CONCLUSIONS: Our results indicated that 4'-O-Demethylophiopogonanone E can be considered as a potential source of therapeutic medicine for inflammatory diseases.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Inflamação/imunologia
Ophiopogon/química
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Inflamação/tratamento farmacológico
Inflamação/genética
Inflamação/fisiopatologia
Interleucina-1beta/genética
Interleucina-1beta/imunologia
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Camundongos
Estrutura Molecular
NF-kappa B/genética
NF-kappa B/imunologia
Células RAW 264.7
Rizoma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Drugs, Chinese Herbal); 0 (Interleukin-1beta); 0 (NF-kappa B)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1539-5


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[PMID]:28049093
[Au] Autor:Chen J; Yuan J; Zhou L; Zhu M; Shi Z; Song J; Xu Q; Yin G; Lv Y; Luo Y; Jia X; Feng L
[Ad] Endereço:Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu 210028, China; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, China.
[Ti] Título:Regulation of different components from Ophiopogon japonicus on autophagy in human lung adenocarcinoma A549Cells through PI3K/Akt/mTOR signaling pathway.
[So] Source:Biomed Pharmacother;87:118-126, 2017 Mar.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Autophagy plays a dual role in the development of cancer, acting as both a tumor suppressor and a cell survival inducer. Ophiopogon japonicus (L.f) Ker-Gawl (OJ), as a traditional Chinese medicine, specially possesses remarkable anti-cancer activity in the clinical. Previously, studies have indicated that flavonoids (FOJ) and steroidal saponins (SSOJ) are the main active substances of OJ. However, the effects of FOJ and SSOJ on autophagy of A549 cells have not been fully elucidated. In this study, we found that the expressions of autophagy-related mediators (LC3-II/LC3-I ratio, Atg-3, Atg-7 and Beclin-1) were increased in A549 cells by the treatment with FOJ (7.9mg crude drug/mL) and SSOJ (12.2mg crude drug/mL). Meanwhile, FOJ or SSOJ could induce the up-regulation of LC3-II at both protein and mRNA levels. Moreover, we observed the cytoplasmic vaculoes which formed double-layered membranes and only some cytoplasmic organelles or myelin figures remained in FOJ or SSOJ-treated A549 cells for 24h by Transmission Electron Microscopy (TEM). Further detection about the PI3K/Akt/mTOR signaling pathway showed that the levels of PI3K, Akt and mTOR were significantly suppressed with the FOJ or SSOJ treatment. The 3-MA (an autophagy inhibitor) and LY294002 (a PI3K inhibitor) further confirmed the underlying mechanism in the FOJ or SSOJ-induced autophagy of A549 cells. Additionally, the pretreatment with FOJ and SSOJ increased the level of p53, whereas decreased the expression of Ki67. These findings suggested that FOJ or SSOJ could activate the autophagy of A549 cells, wherein the mechanism might be associated with their inhibition of PI3K/Akt/mTOR signaling pathway. Thus, FOJ or SSOJ could be a potential autophagy inducer to prevent the process of lung cancer.
[Mh] Termos MeSH primário: Adenocarcinoma/metabolismo
Autofagia/fisiologia
Medicamentos de Ervas Chinesas/farmacologia
Neoplasias Pulmonares/metabolismo
Ophiopogon
Fosfatidilinositol 3-Quinases/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Serina-Treonina Quinases TOR/metabolismo
[Mh] Termos MeSH secundário: Células A549
Autofagia/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/fisiologia
Relação Dose-Resposta a Droga
Medicamentos de Ervas Chinesas/isolamento & purificação
Flavonoides/isolamento & purificação
Flavonoides/farmacologia
Seres Humanos
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores
Saponinas/isolamento & purificação
Saponinas/farmacologia
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/fisiologia
Serina-Treonina Quinases TOR/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Saponins); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.1 (MTOR protein, human); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170710
[Lr] Data última revisão:
170710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


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[PMID]:27916782
[Au] Autor:Dang NH; Chung ND; Tuan HM; Hiep NT; Dat NT
[Ad] Endereço:Advanced Center for Bio-organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST).
[Ti] Título:Cytotoxic Homoisoflavonoids from Ophiopogon japonicus Tubers.
[So] Source:Chem Pharm Bull (Tokyo);65(2):204-207, 2017 Feb 01.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A phytochemical fractionation of a methanol extract of Ophiopogon japonicus tubers led to the isolation of a new homoisoflavanone, homoisopogon A (1), and three new homoisoflavanes, homoisopogon B-D (2-4). Their chemical structures were elucidated by mass, NMR, and circular dichroism (CD) spectroscopic methods. Homoisopogon A (1) exhibited potent cytotoxicity against human lung adenocarcinoma LU-1, human epidermoid carcinoma KB, and human melanoma SK-Mel-2 cancer cells with IC values ranging from 0.51 to 0.66 µM.
[Mh] Termos MeSH primário: Ensaios de Seleção de Medicamentos Antitumorais
Isoflavonas/isolamento & purificação
Isoflavonas/farmacologia
Ophiopogon/química
Extratos Vegetais/química
Tubérculos/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
Isoflavonas/química
Estrutura Molecular
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoflavones); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c16-00743


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[PMID]:27659001
[Au] Autor:Zhu N; Lv X; Wang Y; Li J; Liu Y; Lu W; Yang L; Zhao J; Wang F; Zhang LW
[Ad] Endereço:Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai 201210, China.
[Ti] Título:Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells.
[So] Source:Int J Biol Macromol;93(Pt A):940-951, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Polysaccharides from different types of natural herbs have not been compared with each other to determine their differential potencies on innate immune response, such as maturation of dendritic cells (DC). In addition, the role of endocytosis of polysaccharides in DC maturation has not been explored previously. Polysaccharides isolated from Astragalus membranaceus (APS), Ganoderma lucidum (GLP) and Radix ophiopogonis (OGP) were characterized and applied in bone marrow derived DC. Compared to immature DC, three polysaccharides with immunoactivities showed elongated dendrites, decreased phagocytic abilities, phenotypic changes (CD40/MHCII/CD80/CD86) and increased level of nitric oxide (NO) in a dose dependent manner. Interestingly, blockage of NO by iNOS inhibitor slightly decreased CD40 and MHCII but not CD80/CD86 expression induced by polysaccharides, indicating that NO was partially involved in DC maturation. In addition, GLP can enter cells in a dose and time dependent manner, shown as punctate distribution in the cytoplasm. Endocytic inhibitors sodium azide and brefeldinA that were demonstrated to inhibit cellular uptake of GLP can block phenotypic maturation of DC. Taken together, these results suggested that polysaccharides from natural herbs are effective immunostimulators with variable potencies ranking as GLP>APS>OGP, and the increase of NO level as well as the increase in polysaccharide endocytosis could be the novel strategies for improved innate response and immunotherapy.
[Mh] Termos MeSH primário: Células Dendríticas/imunologia
Fatores Imunológicos/farmacologia
Extratos Vegetais/farmacologia
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Astragalus membranaceus/química
Forma Celular/efeitos dos fármacos
Forma Celular/imunologia
Células Cultivadas
Células Dendríticas/efeitos dos fármacos
Células Dendríticas/metabolismo
Fatores Imunológicos/isolamento & purificação
Masculino
Camundongos Endogâmicos C57BL
Óxido Nítrico/metabolismo
Ophiopogon/química
Fagocitose/efeitos dos fármacos
Extratos Vegetais/isolamento & purificação
Polissacarídeos/isolamento & purificação
Reishi/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Plant Extracts); 0 (Polysaccharides); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE


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[PMID]:27589720
[Au] Autor:Cao G; Jiang N; Hu Y; Zhang Y; Wang G; Yin M; Ma X; Zhou K; Qi J; Yu B; Kou J
[Ad] Endereço:Jiangsu Key Laboratory of Traditional Chinese Medicine Evaluation and Translational Research, Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China. caoguosheng2006@163.com.
[Ti] Título:Ruscogenin Attenuates Cerebral Ischemia-Induced Blood-Brain Barrier Dysfunction by Suppressing TXNIP/NLRP3 Inflammasome Activation and the MAPK Pathway.
[So] Source:Int J Mol Sci;17(9), 2016 Aug 29.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, has been shown to inhibit cerebral ischemic injury. However, its potential molecular action on blood-brain barrier (BBB) dysfunction after stroke remains unclear. This study aimed to investigate the effects of ruscogenin on BBB dysfunction and the underlying mechanisms in middle cerebral artery occlusion/reperfusion (MCAO/R)-injured mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-injured mouse brain microvascular endothelial cells (bEnd.3). The results demonstrated that administration of ruscogenin (10 mg/kg) decreased the brain infarction and edema, improved neurological deficits, increased cerebral brain flow (CBF), ameliorated histopathological damage, reduced evans blue (EB) leakage and upregulated the expression of tight junctions (TJs) in MCAO/R-injured mice. Meanwhile, ruscogenin (0.1-10 µM) treatment increased cell viability and trans-endothelial electrical resistance (TEER) value, decreased sodium fluorescein leakage, and modulated the TJs expression in OGD/R-induced bEnd.3 cells. Moreover, ruscogenin also inhibited the expression of interleukin-1ß (IL-1ß) and caspase-1, and markedly suppressed the expression of Nucleotide-binding domain (NOD)-like receptor family, pyrin domain containing 3 (NLRP3) and thiredoxin-interactive protein (TXNIP) in vivo and in vitro. Furthermore, ruscogenin decreased reactive oxygen species (ROS) generation and inhibited the mitogen-activated protein kinase (MAPK) pathway in OGD/R-induced bEnd.3 cells. Our findings provide some new insights into its potential application for the prevention and treatment of ischemic stroke.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Barreira Hematoencefálica/efeitos dos fármacos
Infarto da Artéria Cerebral Média/tratamento farmacológico
Inflamassomos/metabolismo
Sistema de Sinalização das MAP Quinases
Espirostanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
Barreira Hematoencefálica/metabolismo
Proteínas de Transporte/metabolismo
Caspase 1/genética
Caspase 1/metabolismo
Hipóxia Celular
Linhagem Celular
Infarto da Artéria Cerebral Média/metabolismo
Inflamassomos/efeitos dos fármacos
Interleucina-1beta/genética
Interleucina-1beta/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo
Ophiopogon/química
Espécies Reativas de Oxigênio/metabolismo
Espirostanos/uso terapêutico
Tiorredoxinas/metabolismo
Junções Íntimas/efeitos dos fármacos
Junções Íntimas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Carrier Proteins); 0 (Inflammasomes); 0 (Interleukin-1beta); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (Nlrp3 protein, mouse); 0 (Reactive Oxygen Species); 0 (Spirostans); 0 (Txnip protein, mouse); 52500-60-4 (Thioredoxins); BXI92R2VUJ (ruscogenin); EC 3.4.22.36 (Caspase 1)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE



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