Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.100.060.650 [Categoria DeCS]
Referências encontradas : 123 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 13 ir para página                         

  1 / 123 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29262679
[Au] Autor:Liu Q; Li W; Nagata K; Fu H; Okada S; Tanabe I; Kobori Y; Higai K; Norie W; Sasaki T; Asada Y; Zhao H; Bai H; Koike K
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
[Ti] Título:Isolation, Structural Elucidation, and Liquid Chromatography-Mass Spectrometry Analysis of Steroidal Glycosides from Polygonatum odoratum.
[So] Source:J Agric Food Chem;66(2):521-531, 2018 Jan 17.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rhizomes of Polygonatum odoratum represent a traditional Chinese medicine and functional food. A phytochemical investigation resulted in the isolation of eight steroidal glycosides (1-8), including two new compounds, polygonatumosides F (1) and G (2). The structures were elucidated by spectroscopic data and chemical reactions. Compound 7 showed antiproliferation activity against human hepatocellular carcinoma cell line HepG2 (IC of 3.2 µM). The chemical profile and contents of steroidal glycosides of P. odoratum rhizomes collected at different dates and geographical locations were also investigated, indicating that the rational harvest of P. odoratum in spring and autumn is preferable to obtain higher levels of steroidal glycosides. Compounds 1 and 7 showed the highest contents in all P. odoratum samples and have potential to serve as chemotaxonomic and chemical markers for quality control of this important plant material. 14-Hydroxylation may be a key step for the biosynthesis of compounds 1-7.
[Mh] Termos MeSH primário: Glicosídeos/química
Glicosídeos/isolamento & purificação
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Polygonatum/química
Esteroides/química
Esteroides/isolamento & purificação
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Cromatografia Líquida
Glicosídeos/farmacologia
Células Hep G2
Seres Humanos
Espectrometria de Massas
Estrutura Molecular
Extratos Vegetais/farmacologia
Rizoma/química
Esteroides/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Plant Extracts); 0 (Steroids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04488


  2 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28257664
[Au] Autor:Yan H; Lu J; Wang Y; Gu W; Yang X; Yu J
[Ad] Endereço:Yunnan University of Traditional Chinese Medicine, 1076 Yuhua Road, Chenggong District, Kunming, Yunnan Province, China.
[Ti] Título:Intake of total saponins and polysaccharides from Polygonatum kingianum affects the gut microbiota in diabetic rats.
[So] Source:Phytomedicine;26:45-54, 2017 Mar 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The gut microbiota has been reported to play a critical role in metabolic diseases, including in diabetes. Polygonatum kingianum has been used in the treatment of diabetes and related diseases in China for centuries. Total saponins (TSPK) and total polysaccharides (PSPK) were reported to be major types of active constituents of P. kingianum. This research aims at investigation of their therapeutical mechanisms on diabetes based on the regulation of gut microbiota. STUDY DESIGN: Type 2 diabetes (T2D) rats were induced by high-fat diet (HFD) and streptozotocin-injection from male Sprague-Dawley (SD) rats. The blood biochemical indicators were measured. Intestinal microbial diversities and the overall structural changes in gut microbiota and the contents of the short chain fatty acids (SCFAs) were discussed. METHODS: T2D rats were treated with TSPK (0.025 and 0.1g/kg) and PSPK (0.1g/kg) for 56 days. Major biochemical indexes, such as fasting blood glucose (FBG), fasting insulin (FINS) and lipopolysaccharide (LPS), were measured. Intestinal microbial diversities and the overall structural changes in gut microbiota were discussed based on the sequencing results on V4 region of 16S rDNA. Moreover, the contents of the SCFAs in faeces, which were fermentation products produced from gut microbiota were determined by gas chromatography (GC). RESULTS: Oral administration of TSPK and PSPK prevented the increase of FBG. TSPK (0.025g/kg) enhancing the content of FINS at the end of research. Furthermore, TSPK and PSPK improved the intestinal microecology by decreasing the abundances of Bacteroidetes and Proteobacteria, and increasing that of Firmicutes. However, TSPK.L, PSPK and TSPK.H displayed discrepant regulation roles on Firmicutes. TSPK.L and PSPK significantly increased the abundance of Ruminococcaceae family and Ruminococcus genus in Firmicutes phylum, however, TSPK.H increased the abundances of Veillonellaceae family and Anaerovibrio genus. 57 Key variables, altered after treated by TSPK and PSPK, correlated to the alternations of FBG, FINS, LPS and body weight were identified. In addition, TSPK.L, PSPK and TSPK.H showed different adjustment on the contents of SCFAs. CONCLUSION: These results suggested that, compared to the normal rats, the structure of gut microbiota was significantly changed in diabetic rats. Oral administration with TSPK and PSPK could prevent T2D by its regulation role on the gut microbiota.
[Mh] Termos MeSH primário: Diabetes Mellitus/tratamento farmacológico
Diosgenina/farmacologia
Microbioma Gastrointestinal/efeitos dos fármacos
Hipoglicemiantes/farmacologia
Extratos Vegetais/farmacologia
Polissacarídeos/farmacologia
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Animais
China
Masculino
Polygonatum/química
Ratos
Ratos Sprague-Dawley
Saponinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Plant Extracts); 0 (Polysaccharides); 0 (Saponins); K49P2K8WLX (Diosgenin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  3 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28098871
[Au] Autor:Zhang H; Du X; Sun TT; Wang CL; Li Y; Wu SZ
[Ad] Endereço:Institute of Traditional Chinese Medicine, Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi 710003, P.R. China.
[Ti] Título:Lectin PCL inhibits the Warburg effect of PC3 cells by combining with EGFR and inhibiting HK2.
[So] Source:Oncol Rep;37(3):1765-1771, 2017 Mar.
[Is] ISSN:1791-2431
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Prostatic carcinoma is the most aggressive tumor in adult men. Warburg effect is an important characteristic of tumor cell metabolism including prostate cancer cells, in which hexokinase 2 (HK2), a major rate-limiting enzyme involved in Warburg effect, is selectively upregulated. The lectin PCL is a mannose binding lectin which induces tumor cell apoptosis and autophagy. In the present study, we report that PCL could lower glucose consumption and lactate production, shift the Warburg effect by inhibiting the expression of HK2 in PC3 cells and the suppression of HK2 by siRNA reversed the effect of PCL on glucose consumption and lactate production. The expression of HK2 is closely related to epidermal growth factor receptor (EGFR) and downstream signaling pathway activation, therefore, we investigated the interaction of PCL with EGFR by western blot analysis and found that PCL could suppress the binding of epidermal growth factor (EGF) with EGFR and HK2 expression. Also, we explored the binding mechanism between the PCL and EGFR through molecular docking and molecular dynamics simulations and found that PCL bocked the active site of EGFR which is also the binding site of the nature ligand EGF, the resulting conformation has higher stability than EGF in complex with EGFR. The results indicated that PCL could competitively bind to EGFR binding pocket and then prevent EGF from binding to EGFR, blocking the autophosphorylation of the EGFR tyrosine kinase, after that the EGFR activation is inhibited. Collectively, our studies concluded that PCL inhibits tumor cell glycolysis by combining with EGFR and reducing HK2 expression.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Glicólise/efeitos dos fármacos
Hexoquinase/antagonistas & inibidores
Lectinas/farmacologia
Consumo de Oxigênio/efeitos dos fármacos
Neoplasias da Próstata/tratamento farmacológico
Receptor do Fator de Crescimento Epidérmico/metabolismo
[Mh] Termos MeSH secundário: Adulto
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Western Blotting
Cromatografia Líquida de Alta Pressão
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Masculino
Simulação de Acoplamento Molecular
Polygonatum/química
Neoplasias da Próstata/metabolismo
Neoplasias da Próstata/patologia
Conformação Proteica/efeitos dos fármacos
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptor do Fator de Crescimento Epidérmico/química
Receptor do Fator de Crescimento Epidérmico/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais/efeitos dos fármacos
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Lectins); 0 (RNA, Messenger); EC 2.7.1.1 (Hexokinase); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.3892/or.2017.5367


  4 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27510582
[Au] Autor:Wang Y; Qin S; Pen G; Chen D; Han C; Miao C; Lu B; Su C; Feng S; Li W; Han J; Cho NC; Si Y
[Ad] Endereço:Department of Ophthalmology, Chonbuk National University, Jeollabuk-do 561-756, Republic of Korea (past position).
[Ti] Título:Original Research: Potential ocular protection and dynamic observation of Polygonatum sibiricum polysaccharide against streptozocin-induced diabetic rats' model.
[So] Source:Exp Biol Med (Maywood);242(1):92-101, 2017 Jan.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ocular complications associated with diabetes mellitus are progressive and becoming one of the most important causes of morbidity worldwide. The purpose of the study is to evaluate the protective effect of Polygonatum sibiricum polysaccharide, an important component of Polygonatum sibiricum, on ocular complications in streptozotocin-induced diabetes mellitus rats. Sprague Dawley rats were made diabetic with streptozotocin(60 mg/kg, i.v.) and then the rats were treated with Polygonatum sibiricum polysaccharide 200, 400 and 800 mg/kg.d by gavage for 12 weeks. Biochemical analysis indicated that Polygonatum sibiricum polysaccharide lowered the levels of fasting blood glucose and glycated hemoglobin in blood and elevated the levels of insulin and C-peptide in plasma of diabetes mellitus rats in a dose-dependent manner. Physical measurements revealed that Polygonatum sibiricum polysaccharide improved clinical symptoms of polydipsia, polyphagia, polyuria and weight loss in diabetes mellitus rats. The content of malondialdehyde and activity of superoxide dismutase in plasma were determined, and the data showed Polygonatum sibiricum polysaccharide suppressed oxidative stress reaction. Lens opacification was observed using slit lamp illumination, and the data showed Polygonatum sibiricum polysaccharide delayed cataract progression in a dose-dependent manner. Electroretinogram showed Polygonatum sibiricum polysaccharide treatment reversed the decrease of electroretinogram b and OPs2 waves' amplitudes. Flash-visual evoked potential test indicated that the peak time of P2 wave was prolonged, and the amplitude of N2-P2 was lowered in diabetes mellitus group, and Polygonatum sibiricum polysaccharide suppressed these changes. Fundus fluorescein angiography showed Polygonatum sibiricum polysaccharide alleviated the retinal vasculopathy in a dose-dependent manner. In conclusion, these results suggest that the administration of Polygonatum sibiricum polysaccharide slows the progression of diabetic retinopathy and cataract through alleviating hyperglycemia and reducing oxidative stress in streptozotocin-induced diabetes mellitus rats.
[Mh] Termos MeSH primário: Catarata/tratamento farmacológico
Diabetes Mellitus Experimental/complicações
Hipoglicemiantes/farmacologia
Polygonatum/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Catarata/etiologia
Diabetes Mellitus Experimental/tratamento farmacológico
Diabetes Mellitus Experimental/metabolismo
Retinopatia Diabética/tratamento farmacológico
Eletrorretinografia
Potenciais Evocados Visuais/efeitos dos fármacos
Glucose/metabolismo
Hemoglobina A Glicada/metabolismo
Masculino
Plantas Medicinais/química
Polidipsia/tratamento farmacológico
Polidipsia/etiologia
Poliúria/tratamento farmacológico
Ratos Sprague-Dawley
Estreptozocina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycated Hemoglobin A); 0 (Hypoglycemic Agents); 0 (Polysaccharides); 5W494URQ81 (Streptozocin); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE


  5 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27812188
[Au] Autor:Wang JJ; Yang YP; Sun H; Wen J; Deng T; Nie ZL; Meng Y
[Ad] Endereço:Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, China.
[Ti] Título:The Biogeographic South-North Divide of Polygonatum (Asparagaceae Tribe Polygonateae) within Eastern Asia and Its Recent Dispersals in the Northern Hemisphere.
[So] Source:PLoS One;11(11):e0166134, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eastern Asia (EA) is a key region for the diversification of flowering plants in the Northern Hemisphere, but few studies have focused on the biogeographic history within EA in the context of the other northern continents. Polygonatum is an important medicinal genus widely distributed in the Northern Hemisphere with its highest species richness in EA, and it represents an excellent model for studying the evolution of biogeographic patterns in this region. Divergence time estimation was used to examine the biogeographic history of Polygonatum based on nuclear ITS and four plastid sequences (rbcL, matK, psbA-trnH and trnC-petN) from 30 Polygonatum species and 35 outgroup taxa. The ancestral area of Polygonatum and subsequent dispersal routes were inferred using Bayes-Lagrange. Polygonatum was estimated to have originated in southern EA during the middle Miocene (14.34-13.57 Ma) with subsequent south-to-north expansion in the late Miocene. Multiple intercontinental dispersal events were inferred between EA and Europe or North America, and all of them have occurred recently in the late Miocene to Pliocene. The separation of Polygonatum into the south and north lineages and their subsequent diversifications in the late Miocene supports the existence of a biogeographic divide between the northern and southern parts of EA that also coincides with the retreat and redevelopment of the arid zone in EA in the Neogene. Our results demonstrate the complexity of biogeographic history of Polygonatum in the Northern Hemisphere including early vicariance followed by frequent and recent dispersals in the Neogene.
[Mh] Termos MeSH primário: Geografia
Dispersão Vegetal
Polygonatum/classificação
Polygonatum/fisiologia
[Mh] Termos MeSH secundário: Teorema de Bayes
Biodiversidade
Extremo Oriente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166134


  6 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27644624
[Au] Autor:Han SY; Hu MH; Qi GY; Ma CX; Wang YY; Ma FL; Tao N; Qin ZH
[Ad] Endereço:Institute of Biophysics, Chinese Academy of Science, Beijing, China E-mail : zhihai@ibp.ac.cn; tao@moon.ibp.ac.cn.
[Ti] Título:Polysaccharides from Polygonatum Inhibit the Proliferation of Prostate Cancer-Associated Fibroblasts.
[So] Source:Asian Pac J Cancer Prev;17(8):3829-33, 2016.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:Inhibition of cancer-associated broblasts (CAFs) may improve the efficacy of cancer therapy. Polysaccharide extracted from polygonatum can selectively inhibit the growth of prostate-CAFs (<.001) without inhibiting the growth of normal broblasts (NAFs). Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs. 3-methyl-adenine(3-MA) is an autophagy inhibitor. 3-MA was added to prostate-CAFs with polysaccharide from polygonatum to determine whether autophagy plays an important role in the restrained effect. Finally, polysaccharide from polygonatum treatment significantly increased the activation of Beclin-1 and LC3, key autophagy proteins. Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs and inhibits prostate-CAF growth, indicating that a novel anti-cancer strategy involves inhibiting the growth of prostate- CAFs.
[Mh] Termos MeSH primário: Fibroblastos Associados a Câncer/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Polygonatum/química
Polissacarídeos/farmacologia
Neoplasias da Próstata/tratamento farmacológico
[Mh] Termos MeSH secundário: Proteínas Reguladoras de Apoptose/metabolismo
Autofagia/efeitos dos fármacos
Fibroblastos Associados a Câncer/metabolismo
Linhagem Celular Tumoral
Seres Humanos
Masculino
Proteínas de Membrana/metabolismo
Extratos Vegetais/farmacologia
Próstata/efeitos dos fármacos
Neoplasias da Próstata/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Membrane Proteins); 0 (Plant Extracts); 0 (Polysaccharides)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


  7 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27324797
[Au] Autor:Lee JE; Kim EJ; Kim MH; Hong J; Yang WM
[Ad] Endereço:Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
[Ti] Título:Polygonatum stenophyllum improves menopausal obesity via regulation of lipolysis-related enzymes.
[So] Source:J Nat Med;70(4):789-96, 2016 Oct.
[Is] ISSN:1861-0293
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Menopausal women are associated with an increase in obesity accompanying changes in the body's condition and composition. Polygonatum stenophyllum (PS) rhizome, which is a traditional herbal remedy, has been used to treat various diseases including obesity. However, the effect of PS on menopausal obesity remains unclear. Female C57BL/6 mice were ovariectomized (OVX) and fed on high fat diet (HFD) to induce menopausal obesity. Aqueous extract of PS of 1, 10, and 100 mg/kg was orally administrated for 6 weeks after 7 weeks of induction. The weights of body, uterine, and ovarian fat were investigated. Histological analysis was performed to monitor the changes of liver and fat. In addition, lipid profiles were measured in serum and the expression of lipolysis-related enzymes was analyzed in uterine tissues. PS significantly decreased the weights of body and ovarian fat and increased the uterine weight compared to control group. Administration of PS significantly decreased the adipocyte diameter and the lipid droplets within the hepatocytes. In addition, PS-treated mice had lower levels of triglyceride (TG), total cholesterol, and LDL-cholesterol than control mice. The expression of lipolysis-related genes, including adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), was increased in PS-treated groups. Taken together, these results demonstrate that PS might have efficacy on menopausal obesity by activating ATGL and HSL.
[Mh] Termos MeSH primário: Tecido Adiposo/metabolismo
Fármacos Antiobesidade/farmacologia
Lipólise/efeitos dos fármacos
Obesidade/enzimologia
Fitoterapia
Extratos Vegetais/farmacologia
Polygonatum
[Mh] Termos MeSH secundário: Adipócitos/metabolismo
Animais
Fármacos Antiobesidade/uso terapêutico
Dieta Hiperlipídica
Feminino
Lipase/metabolismo
Menopausa
Camundongos
Camundongos Endogâmicos C57BL
Obesidade/tratamento farmacológico
Obesidade/etiologia
Ovariectomia
Extratos Vegetais/uso terapêutico
Rizoma
Esterol Esterase/metabolismo
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Obesity Agents); 0 (Plant Extracts); 0 (Triglycerides); EC 3.1.1.13 (Sterol Esterase); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1007/s11418-016-1018-9


  8 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27318358
[Au] Autor:Liu T; Wu L; Wang D; Wang H; Chen J; Yang C; Bao J; Wu C
[Ad] Endereço:School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610064, China.
[Ti] Título:Role of reactive oxygen species-mediated MAPK and NF-κB activation in polygonatum cyrtonema lectin-induced apoptosis and autophagy in human lung adenocarcinoma A549 cells.
[So] Source:J Biochem;160(6):315-324, 2016 Dec.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding lectin isolated from the rhizomes of Polygonatum cyrtonema Hua, has been reported to possess remarkable anti-tumour effects via inducing apoptosis and autophagy. The aim of this study was to investigate the molecular mechanisms mediating PCL-induced apoptosis and autophagy in A549 cells. Herein, we found that the treatment of A549 cells with PCL caused a remarkable generation of reactive oxygen species (ROS) and ROS scavenger N-acetyl-cysteine (NAC) inhibited PCL-induced apoptosis and autophagy. In addition, PCL treatment activated mitogen-activated protein kinase (MAPK) members extracellular signal-regulated kinase (ERK), JNK and p38, JNK inhibitor and p38 inhibitor partially reduced PCL-induced apoptosis and autophagy. Moreover, PCL administration activated NF-κB survival pathway in A549 cells, NF-κB inhibitor Bay11-7082 promoted PCL-induced apoptosis. Importantly, we found PCL may bind to the cell surface in a mannose-specific manner, and was then internalized and accumulated primarily onto the mitochondria. These findings may provide a new perspective of PCL as a potential anti-tumour drug targeting apoptosis and autophagy pathways for future cancer therapeutics.
[Mh] Termos MeSH primário: Adenocarcinoma/metabolismo
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Neoplasias Pulmonares/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
NF-kappa B/metabolismo
Lectinas de Plantas/farmacologia
Polygonatum/química
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
Lectinas de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (Plant Lectins); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


  9 / 123 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26841079
[Au] Autor:Wang J; Lu CS; Liu DY; Xu YT; Zhu Y; Wu HH
[Ad] Endereço:a Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis , Tianjin University of Traditional Chinese Medicine , Tianjin , 300457 , China.
[Ti] Título:Constituents from Polygonatum sibiricum and their inhibitions on the formation of advanced glycosylation end products.
[So] Source:J Asian Nat Prod Res;18(7):697-704, 2016 Jul.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new saponin, isonarthogenin 3-O-ß-d-glucopyranosyl-(1→4)-ß-d-galactopyranoside (1), together with twelve known compounds, were isolated from the rhizomes of Polygonatum sibiricum. The structures of these compounds were elucidated by analysis of their 1D/2D NMR and MS data. Among them, phenol compounds 4-7 and 9-10 showed significant inhibitions against the formation of advanced glycosylation end products, with IC50 values of 0.091 ± 0.0021, 0.10 ± 0.041, 0.014 ± 0.0027, 0.11 ± 0.011, 0.13 ± 0.045, and 0.055 ± 0.019 µM, respectively. The results will promote exploiting potential medicinal use of these compounds in the prevention of diabetic complications and supporting Polygonatum sibiricum as a functional food for healthy and medicinal diet.
[Mh] Termos MeSH primário: Produtos Finais de Glicação Avançada/antagonistas & inibidores
Polygonatum/química
Saponinas/isolamento & purificação
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Medicamentos de Ervas Chinesas/química
Seres Humanos
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Rizoma/química
Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Glycation End Products, Advanced); 0 (Saponins); 0 (isonarthogenin 3-O-beta-D-glucopyranosyl-(1-4)-beta-D-galactopyranoside)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2015.1135905


  10 / 123 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26743227
[Au] Autor:Lu JM; Wang YF; Yan HL; Lin P; Gu W; Yu J
[Ad] Endereço:Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Department of Pharmacy, Yunnan University of Traditional Chinese Medicine, 1076 Yuhua Road, Chenggong District, Kunming, Yunnan Province, China.
[Ti] Título:Antidiabetic effect of total saponins from Polygonatum kingianum in streptozotocin-induced daibetic rats.
[So] Source:J Ethnopharmacol;179:291-300, 2016 Feb 17.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Polygonatum kingianum has been used in the prevention and treatment of diabetes, hyperlipidemia and related metabolic syndrome in Asian counties for centuries. In this study, the blood glucose regulation activity and mechanism of total saponins from P. kingianum (TSPK) were investigated in streptozotocin (STZ)-induced diabetic rats in this research. METHODS: TSPK (0.025g/kg and 0.1mg/kg) was administrated by gavage to STZ-induced diabetic rats for 8 weeks. Changes of body weight, food intakes, blood glucose, serum insulin and lipid indexes were observed. Genome-wide expression profiling was applied to explore the gene expression alternation after treated with TSPK. Expressions of adenosine monophosphate activated protein kinase (AMPK), phosphoenolpyruvate carboxykinase (PEPCK), the relative transcript level of glucose kinase and glucose-6-phosphatase (GK/G6P) in the liver were investigated. Meanwhile, contents of AMPK, and glucose transporter subtype-4 (GLUT4) in skeletal muscle, and peroxysome proliferator-activated receptor (PPAR-γ) in adipose tissue were investigated. RESULTS: TSPK could effectively alleviate hyperglycemia and hyperlipidemia in diabetic rats. Genome-wide expression profiling showed that TSPK up-regulated the expression of GLUT4 while down-regulated the expression of G6P in insulin signal pathway. In the liver, the expression of AMPK and GK are increased. Further more, TSPK promoted the expressions of GLUT4 in skeletal muscle, and PPAR-γ in adipose tissue, respectively. CONCLUSION: These results provide possible mechanisms for the antidiabetic effects of TSPK. TSPK could promote not only glycogenesis but also glucose utilization in peripheral tissue. Our results suggested that TSPK may be used as adjuvant therapy to control blood glucose and insulin resistance in type 2 diabetic individuals.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/tratamento farmacológico
Polygonatum
Saponinas/isolamento & purificação
Saponinas/uso terapêutico
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/metabolismo
Tecido Adiposo/efeitos dos fármacos
Animais
Glicemia/metabolismo
Diosgenina
Relação Dose-Resposta a Droga
Expressão Gênica/efeitos dos fármacos
Transportador de Glucose Tipo 4/metabolismo
Glucose-6-Fosfatase/metabolismo
Hipoglicemiantes/isolamento & purificação
Hipoglicemiantes/farmacologia
Hipoglicemiantes/uso terapêutico
Insulina/sangue
Lipídeos/sangue
Fígado/metabolismo
Masculino
Músculo Esquelético/metabolismo
PPAR gama/metabolismo
Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo
Ratos
Saponinas/farmacologia
Transdução de Sinais/efeitos dos fármacos
Estreptozocina
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glucose Transporter Type 4); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Lipids); 0 (PPAR gamma); 0 (Saponins); 5W494URQ81 (Streptozocin); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 3.1.3.9 (Glucose-6-Phosphatase); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)); K49P2K8WLX (Diosgenin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE



página 1 de 13 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde