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Pesquisa : B01.650.940.800.575.912.250.618.100.060.810 [Categoria DeCS]
Referências encontradas : 23 [refinar]
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  1 / 23 MEDLINE  
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[PMID]:26140544
[Au] Autor:Hafez Ghoran S; Saeidnia S; Babaei E; Kiuchi F; Hussain H
[Ad] Endereço:a Faculty of Basic Sciences, Department of Chemistry , Golestan University , Gorgan 4913815739 , Iran.
[Ti] Título:Scillapersicene: a new homoisoflavonoid with cytotoxic activity from the bulbs of Scilla persica HAUSSKN.
[So] Source:Nat Prod Res;30(11):1309-14, 2016 Jun.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The phytochemical investigation of Scilla persica HAUSSKN bulbs led to the isolation of a novel homoisoflavonoid that named Scillapersicene (1) and identified as 3-(3',4'-dihydroxybenzylidene)-8-hydroxy-5,7-dimethoxychroman-4-one along with five known homoisoflavonoids 2-6, whose structures were elucidated using HRFAB-MS, 1D and 2D NMR spectroscopic data. The known compounds were identified as 3-(3',4'-dihydroxybenzyl)-5,8-dihydroxy-7-methoxychroman-4-one (2), 3,9-dihydro-autumnalin (3), autumnalin (4), 3-(3',4'-dihydroxybenzylidene)-5,8-dihydroxy-7-methoxychroman-4-one (5) and scillapersicone (6). All compounds obtained, expect 2 and 4, showed strong cytotoxic activity against AGS cell line. The toxicity on AGS cell line was measured by 1, 3, 5 and 6 with IC50 values of 8.4, 30.5, 10.7 and 24.2 µM, respectively. In addition, the physico-chemical properties of these natural compounds were optimised using density functional method (B3LYP) with standard 6-311+G* basis set. These natural products have low-energy gaps between the first ionisation potentials and highest occupied molecular orbital. In conclusion, the low-energy gap could cause reason for cytotoxic activity of homoisoflavonoids.
[Mh] Termos MeSH primário: Isoflavonas/toxicidade
Scilla/química
[Mh] Termos MeSH secundário: Produtos Biológicos/análise
Morte Celular/efeitos dos fármacos
Linhagem Celular
Elétrons
Seres Humanos
Concentração Inibidora 50
Isoflavonas/química
Isoflavonas/isolamento & purificação
Isoflavonas/farmacologia
Espectroscopia de Ressonância Magnética
Modelos Químicos
Estrutura Molecular
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Isoflavones); 0 (scillapersicene)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150704
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1054286


  2 / 23 MEDLINE  
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[PMID]:25612441
[Au] Autor:Wang YM; Fan MY; Li J; Wang ZM; Gao HM
[Ti] Título:[Homoisoflavanones and stilbenes from fresh bulb of Scilla scilloides].
[So] Source:Zhongguo Zhong Yao Za Zhi;39(19):3788-93, 2014 Oct.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Mian-Zao-Er was collected from the bulbs of Scilla scilloides (Lindl. ) Druce, belonging to the Hyacinthaceae family. 17 compounds were obtained using various column chromatographies on macroporus resin (HPD100), silica gel, Sephadex LH-20 and ODS, as well as semi-preparative HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral data as 2-hydroxy-7-methoxyscillascillin (1), scillascillin (2), 5,7-dihydroxy-3',4'-dimethoxyspiro 2H-1-benzopyran-7'-bicyclo[4.2.0 ] octa [1,3,5 ] -trien } -4-one (3), socialinone (4), 4-methylresveratrol (5), (E)-resveratrol (6), scillavoneA (7), 3,9-di- hydroeucomnalin (8), 3-(3-hydroxy-4-methoxybenzyl) -5,7-dihydroxychroman-4-one (9), (3R)-5,7,3'-trihydroxy-4'-methoxyspiro (2H-1-benzopyran-7'-bicyclo[4, 2, 0] octa [1, 3, 5]-trien} -4-one (10), scillabene A (11), 2-hydroxyscillascillin (12), 3-(4-hydroxybenzyl) -5,7-dihydroxychroman-4-one (13), 3-( 4-hydroxybenzylidene) -5, 7-dihydroxychroman-4-one (14), 3-( 4-hydroxybenzyl) -5-hydroxy-7,8-dimethoxychroman-4-one (15), 3-(4-hydroxybenzyl) -5-hydroxy-6, 7-dimethoxychroman-4-one (16), and 3-(4-hydroxybenzyl)-5,8-hydroxy-7-methoxychroman-4-one (17). Among them, compounds 3, 4, 6, 9, 13 and 15-17 were isolated from this plant for the first time.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/química
Flores/química
Isoflavonas/química
Scilla/química
Estilbenos/química
[Mh] Termos MeSH secundário: Espectrometria de Massas
Estrutura Molecular
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Isoflavones); 0 (Stilbenes); 4737-27-3 (isoflavanone)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:150123
[Lr] Data última revisão:
150123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150124
[St] Status:MEDLINE


  3 / 23 MEDLINE  
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[PMID]:25181677
[Au] Autor:Hafez Ghoran S; Saeidnia S; Babaei E; Kiuchi F; Dusek M; Eigner V; Dehno Khalaji A; Soltani A; Ebrahimi P; Mighani H
[Ad] Endereço:Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Chemistry, Faculty of Sciences, Golestan University, Gorgan 4913815759, Iran. Electronic address: S.Hafezghoran@gu.ac.ir.
[Ti] Título:Biochemical and biophysical properties of a novel homoisoflavonoid extracted from Scilla persica HAUSSKN.
[So] Source:Bioorg Chem;57:51-6, 2014 Dec.
[Is] ISSN:1090-2120
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study isolation and structural elucidation of a homoisoflavonoid, 3-(3',4'-dihydroxybenzyl)-8-hydroxy-5,7-dimethoxychroman-4-one (Scillapersicone 1), is reported from Scilla persica HAUSSKN. The structure was solved by a single crystal X-ray analysis. The unit cell parameters are a=11.7676 (2)Å, b=20.1174 (4)Å, c=7.8645 (9)Å, ß=93.544 (2)°, V=1858.23 (7)Å(3), monoclinic space group P21/c and four symmetry equivalent molecules in an unit cell. The structure was consistent with the UV, IR, 1D and 2D NMR, HRFAB-MS data. The optimized molecular geometry agrees closely that obtained from the single crystal X-ray crystallography. Furthermore, cytotoxicity of this compound was evaluated by MTT assay on AGS and WEHI-164 cancerous cell lines.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Flavonoides/química
Flavonoides/farmacologia
Scilla/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Cristalografia por Raios X
Flavonoides/isolamento & purificação
Seres Humanos
Modelos Moleculares
Neoplasias/tratamento farmacológico
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141203
[Lr] Data última revisão:
141203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140903
[St] Status:MEDLINE


  4 / 23 MEDLINE  
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[PMID]:25026344
[Au] Autor:Nishida Y; Sugahara S; Wada K; Toyohisa D; Tanaka T; Ono M; Yasuda S
[Ad] Endereço:Department of Bioscience, School of Agriculture, Tokai University , Kawayo, Minamiaso, Aso, Kumamoto , Japan and.
[Ti] Título:Inhibitory effects of the ethyl acetate extract from bulbs of Scilla scilloides on lipoxygenase and hyaluronidase activities.
[So] Source:Pharm Biol;52(10):1351-7, 2014 Oct.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal inflammation; however, the medicinal properties of this plant have not been completely established. OBJECTIVE: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the inflammation model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested. MATERIALS AND METHODS: Lipoxygenase and hyaluronidase inhibition of ethyl acetate extract from the bulbs of this plant within 2000 µg/mL or homoisoflavones within 1000 µM were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50 µM homoisoflavones plus lipopolysaccharide (LPS) for 24 h. The culture media were collected and analyzed for determination of the nitric oxide (NO) level by the colorimetric Griess method to measure the extent of inflammation. RESULTS: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC50 values 31.5 and 169 µg/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500 µM. Calculated IC50 values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8 µM as the IC50 value. In the hyaluronidase assay, all homoisoflavones tested at 1000 µM demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50 µM significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model. DISCUSSION AND CONCLUSION: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Hialuronoglucosaminidase/antagonistas & inibidores
Inibidores de Lipoxigenase/farmacologia
Extratos Vegetais/farmacologia
Raízes de Plantas
Scilla
[Mh] Termos MeSH secundário: Acetatos/isolamento & purificação
Animais
Bovinos
Relação Dose-Resposta a Droga
Seres Humanos
Hialuronoglucosaminidase/metabolismo
Lipoxigenase/metabolismo
Inibidores de Lipoxigenase/isolamento & purificação
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Lipoxygenase Inhibitors); 0 (Plant Extracts); 76845O8NMZ (ethyl acetate); EC 1.13.11.12 (Lipoxygenase); EC 3.2.1.35 (Hyaluronoglucosaminidase)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:140911
[Lr] Data última revisão:
140911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140716
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2014.891140


  5 / 23 MEDLINE  
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[PMID]:24761625
[Au] Autor:Fan MY; Wang YM; Wang ZM; Gao HM
[Ti] Título:[Advances on chemical constituents and pharmacological activity of genus Scilla].
[So] Source:Zhongguo Zhong Yao Za Zhi;39(2):162-70, 2014 Jan.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:The genus Scilla consists of 90 species widely distributed in Europe, Asia and Africa, one and its variant of which can be found in China Some species of the genus have been used in traditional medicine to treat various diseases related to inflammation and pain. Phytochemical studies have demonstrated the presence of triterpene and tritepenoid saponins derived from eucosterol, bufadienolides, alkaloids, stilbenoids and lignan in the plants of this genus. Various bioactivities such as antimicrobial, anti-inflammatory, antioxidant, anti-tumor and glycosidase inhibitory activities, have been reported. In this review, the advance of chemical constituents and pharmacological activities of the Scilla species are summarized for further development and utilization of the resource.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Scilla/química
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:140425
[Lr] Data última revisão:
140425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140426
[St] Status:MEDLINE


  6 / 23 MEDLINE  
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[PMID]:24047085
[Au] Autor:Nishida Y; Wada K; Toyohisa D; Tanaka T; Ono M; Yasuda S
[Ad] Endereço:a Department of Bioscience , School of Agriculture, Tokai University , Kawayo, Minamiaso, Aso, Kumamoto , 869-1404 , Japan.
[Ti] Título:Homoisoflavones as the antioxidants responsible from bulbs of Scilla scilloides.
[So] Source:Nat Prod Res;27(24):2360-2, 2013.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Scilla scilloides Druce has been used as a folk medicine to treat dermal inflammation; however, the medicinal property of this plant remains to be entirely clarified. The ethyl acetate extract prepared from bulbs of S. scilloides exhibited antioxidative activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydrogen peroxide (H2O2) and nitric oxide (NO) scavenging assays. Nine homoisoflavones (1-9) yielded from this extract were further examined for their antioxidative activities. Among these chemicals tested, five homoisoflavones (1-3, 5 and 7), six homoisoflavones (1-3 and 5-7) and two homoisoflavones (4 and 5) resulted in showing higher activities than the others in DPPH radical, H2O2 and NO scavenging assays, respectively. Calculated EC50 values indicate 3 as the strongest in the DPPH radical scavenging analysis. These results may indicate a potential role of S. scilloides for its medicinal use and homoisoflavones as the antioxidants responsible.
[Mh] Termos MeSH primário: Antioxidantes/química
Isoflavonas/química
Extratos Vegetais/química
Raízes de Plantas/química
Scilla/química
[Mh] Termos MeSH secundário: Compostos de Bifenilo/química
Estrutura Molecular
Picratos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Isoflavones); 0 (Picrates); 0 (Plant Extracts); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:161128
[Lr] Data última revisão:
161128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130920
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2013.830218


  7 / 23 MEDLINE  
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[PMID]:23877615
[Au] Autor:Yasuda S; Nishida Y; Wada K; Akiyama D; Toyohisa D; Tanaka T; Igoshi K; Ono M
[Ad] Endereço:Department of Bioscience, School of Agriculture, Tokai University, Kyoto 606-8502, Japan.
[Ti] Título:Anti-inflammatory and antioxidative effects of a methanol extract from bulbs of Scilla scilloides.
[So] Source:Biosci Biotechnol Biochem;77(7):1569-71, 2013.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The extract from bulbs of Scilla scilloides exhibited inhibitory effects in lipoxygenase and hyaluronidase assays and various oxidation models in vitro. Incubating the cells in the presence of this extract ameliorated t-butyl hydroperoxide-induced cytotoxicity from 27% to 57% in a macrophage model. The results may indicate the potential role of S. scilloides for its anti-inflammatory and antioxidative effects.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Metanol/química
Caules de Planta/química
Scilla/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/isolamento & purificação
Linhagem Celular
Macrófagos/metabolismo
Camundongos
Estresse Oxidativo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:130723
[Lr] Data última revisão:
130723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130724
[St] Status:MEDLINE


  8 / 23 MEDLINE  
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[PMID]:23649203
[Au] Autor:Ono M; Ochiai T; Yasuda S; Nishida Y; Tanaka T; Okawa M; Kinjo J; Yoshimitsu H; Nohara T
[Ad] Endereço:School of Agriculture, Tokai University, 5435 Minamiaso, Aso, Kumamoto 869-1404, Japan. mono@agri.u-tokai.ac.jp
[Ti] Título:Five new nortriterpenoid glycosides from the bulbs of Scilla scilloides.
[So] Source:Chem Pharm Bull (Tokyo);61(5):592-8, 2013.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Five new norlanostane-type triterpenoid glycosides were isolated from the bulbs of Scilla scilloides DRUCE (Liliaceae). Their chemical structures were determined on the basis of spectroscopic data as well as chemical evidence.
[Mh] Termos MeSH primário: Glicosídeos/isolamento & purificação
Raízes de Plantas/química
Scilla/química
Triterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Glicosídeos/química
Estrutura Molecular
Estereoisomerismo
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Triterpenes)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:130507
[Lr] Data última revisão:
130507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130508
[St] Status:MEDLINE


  9 / 23 MEDLINE  
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[PMID]:23621392
[Au] Autor:Maggiori E; Bartoletti E; Mammucari M
[Ad] Endereço:1 Clinical Research of the Service of Aesthetic Medicine , Ospedale Generale San Giovanni Calibita Fatebenefratelli, Isola Tiberina, Rome, Italy .
[Ti] Título:Intradermal therapy (mesotherapy) with lymdiaral in chronic venous insufficiency and associated fibrosclerotic edema damage: a pilot study.
[So] Source:J Altern Complement Med;19(9):777-81, 2013 Sep.
[Is] ISSN:1557-7708
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Chronic venous insufficiency (CVI) induces alterations that cause fibrosclerotic edema of the subcutaneous tissue. This study examined the effects of a complex naturopathic compound with vasoactive and antiedema activities (Lymdiaral®) administered intradermally. PATIENTS: 40 patients with signs and symptoms of CVI and associated fibrosclerotic edema of the subcutaneous tissue. OUTCOME MEASURES: Efficacy was assessed by using clinical investigation, subjective and objective measures, and ultrasonography performed at baseline and after treatment. RESULTS: Thirty-four patients completed the study; 6 of the original 40 (15%) had stopped for reasons unrelated to study treatment. The treatment was well tolerated. Fifteen adverse reactions were reported among a total of 378 doses administered (3.97%). None of these reactions were severe or required discontinuation of treatment. Subjective symptoms and objective measures improved, and ultrasonography showed statistically significant changes in hypodermal thickness of the medial aspect of the knees. CONCLUSIONS: Its open-label design and small sample size notwithstanding, this study indicates that intradermal therapy, according to the recommendation of the Italian Society of Mesotherapy, may provide a valuable contribution to the treatment of CVI and related fibrosclerotic edema of the subcutaneous tissue by prolonging the local effect of the pharmacologically active compounds. Comparative studies are needed to identify the broader clinical and economic benefits of local therapy compared with other systemic therapies.
[Mh] Termos MeSH primário: Edema/tratamento farmacológico
Mesoterapia/métodos
Fitoterapia/métodos
Preparações de Plantas/farmacologia
Insuficiência Venosa/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Doença Crônica
Conium
Feminino
Seres Humanos
Hydrastis
Perna (Membro)/irrigação sanguínea
Phytolacca
Projetos Piloto
Estudos Prospectivos
Scilla
Resultado do Tratamento
Viscum album
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lymdiaral); 0 (Plant Preparations)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:130909
[Lr] Data última revisão:
130909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130430
[St] Status:MEDLINE
[do] DOI:10.1089/acm.2012.0734


  10 / 23 MEDLINE  
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[PMID]:23127647
[Au] Autor:Pillay P; Phulukdaree A; Chuturgoon AA; Du Toit K; Bodenstein J
[Ad] Endereço:Discipline of Pharmaceutical Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
[Ti] Título:The cytotoxic effects of Scilla nervosa (Burch.) Jessop (Hyacinthaceae) aqueous extract on cultured HepG2 cells.
[So] Source:J Ethnopharmacol;145(1):200-4, 2013 Jan 09.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Bulbs of Scilla nervosa, a medicinal plant indigenous to Southern Africa, are traditionally used in aqueous decoctions to treat a diverse range of illnesses. The bulbs contain homoisoflavanones and stilbenoids. Little information is known about the plant's toxicity on the liver, a major detoxifying organ. This study investigated the effects of an aqueous extract of the bulbs in cultured HepG2 liver cells, a model system for investigating the toxicity of xenobiotics. MATERIALS AND METHODS: The concentration that reduced cell viability to 50% (IC(50)) after 24h treatment was derived. Potential mechanisms of toxicity using the IC(50) were investigated as changes in metabolic activity, apoptosis, oxidative damage and DNA fragmentation. In addition, cytochrome P450 3A4 (CYP3A4) activity, which is implicated in drug metabolism and interactions, was also assayed. RESULTS: Cell viability decreased in a concentration-dependent manner and the IC(50) was determined as 0.03 mg/mL. Treating the cells at the IC(50) for 24h resulted in increased intracellular ATP levels, no significant change in phosphatidylserine externalisation, increased caspase-8 activity, decreased caspase-9 activity, no significant change in mitochondrial membrane potential, increased lipid peroxidation, evidence for genotoxicity as demonstrated by DNA fragmentation, and slightly induced CYP3A4 activity. CONCLUSION: Results suggest that liver cells are sensitive to an aqueous extract of the bulbs and there is an increased potential to induce apoptosis, oxidative stress and genotoxicity in vitro.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Citotoxinas/farmacologia
Extratos Vegetais/toxicidade
Scilla/química
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Apoptose/efeitos dos fármacos
Caspase 8/metabolismo
Caspase 9/metabolismo
Células Cultivadas
Citocromo P-450 CYP3A/metabolismo
Citotoxinas/química
Fragmentação do DNA/efeitos dos fármacos
Relação Dose-Resposta a Droga
Células Hep G2
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Fosfatidilserinas/metabolismo
Raízes de Plantas/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytotoxins); 0 (Phosphatidylserines); 0 (Plant Extracts); 059QF0KO0R (Water); 8L70Q75FXE (Adenosine Triphosphate); EC 1.14.14.1 (Cytochrome P-450 CYP3A); EC 3.4.22.- (Caspase 8); EC 3.4.22.- (Caspase 9)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:140210
[Lr] Data última revisão:
140210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121107
[St] Status:MEDLINE



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