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Referências encontradas : 111 [refinar]
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[PMID]:28497409
[Au] Autor:Lledías F; Hernández F; Rivas V; García-Mendoza A; Cassab GI; Nieto-Sotelo J
[Ad] Endereço:Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad # 2001, Col. Chamilpa C.P., 62210, Cuernavaca, Morelos, Mexico.
[Ti] Título:A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis.
[So] Source:Protein J;36(4):308-321, 2017 Aug.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Crassulacean acid metabolism plants have some morphological features, such as succulent and reduced leaves, thick cuticles, and sunken stomata that help them prevent excessive water loss and irradiation. As molecular constituents of these morphological adaptations to xeric environments, succulent plants produce a set of specific compounds such as complex polysaccharides, pigments, waxes, and terpenoids, to name a few, in addition to uncharacterized proteases. Since all these compounds interfere with the analysis of proteins by electrophoretic techniques, preparation of high quality samples from these sources represents a real challenge. The absence of adequate protocols for protein extraction has restrained the study of this class of plants at the molecular level. Here, we present a rapid and reliable protocol that could be accomplished in 1 h and applied to a broad range of plants with reproducible results. We were able to obtain well-resolved SDS/PAGE protein patterns in extracts from different members of the subfamilies Agavoideae (Agave, Yucca, Manfreda, and Furcraea), Nolinoideae (Dasylirion and Beucarnea), and the Cactaceae family. This method is based on the differential solubility of contaminants and proteins in the presence of acetone and pH-altered solutions. We speculate about the role of saponins and high molecular weight carbohydrates to produce electrophoretic-compatible samples. A modification of the basic protocol allowed the analysis of samples by bidimensional electrophoresis (2DE) for proteomic analysis. Furostanol glycoside 26-O-ß-glucosidase (an enzyme involved in steroid saponin synthesis) was successfully identified by mass spectrometry analysis and de novo sequencing of a 2DE spot from an Agave attenuata sample.
[Mh] Termos MeSH primário: Extração Líquido-Líquido/métodos
Folhas de Planta/química
Proteínas de Plantas/isolamento & purificação
Proteômica/métodos
beta-Glucosidase/isolamento & purificação
[Mh] Termos MeSH secundário: Acetona/química
Agave/química
Asparagaceae/química
Cactaceae/química
Eletroforese em Gel Bidimensional
Espectrometria de Massas
Solventes/química
Yucca/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Solvents); 1364PS73AF (Acetone); EC 3.2.1.- (furostanol glycoside 26-O-beta-glucosidase); EC 3.2.1.21 (beta-Glucosidase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-017-9720-3


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[PMID]:28089097
[Au] Autor:Sastre F; Ferreira F; Pedreschi F
[Ad] Endereço:Departamento de Ingeniería Química y Bioprocesos, Escuela de Ingeniería, Pontificia Universidad Católica de Chile (PUC), Av. Vicuña Mackenna 4860, Santiago de Chile, 7820436, Chile. Electronic address: fsastre@uc.cl.
[Ti] Título:A systematic approach for the chromatographic fractionation and purification of major steroid saponins in commercial extracts of Yucca schidigera Roezl.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1046:235-242, 2017 Mar 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Yucca schidigera Roezl. (yucca) is one of the major industrial sources of steroid saponins used as animal and human food additives. This work describes a new, systematic and reproducible three-step method by medium and high-pressure liquid chromatography (under RP, NP and RP conditions), for the isolation and purification of three groups of saponins, which were further purified in six sub-fractions, and finally into twelve individual steroid saponins previously reported in Y. schidigera. In accordance to the increasing applications of yucca extracts, further analytical, biological and physicochemical studies are still required. The presented method is applicable to the preparation of steroids saponins previously reported in commercial extracts of Y. schidigera, both as highly purified mixtures of defined composition, including twelve pure components.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Extratos Vegetais/química
Saponinas/análise
Saponinas/isolamento & purificação
Yucca/química
[Mh] Termos MeSH secundário: Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Saponins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE


  3 / 111 MEDLINE  
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[PMID]:27979085
[Au] Autor:Tenon M; Feuillère N; Roller M; Birtic S
[Ad] Endereço:Naturex SA, Site d'Agroparc BP 1218, 84911 Avignon Cedex 9, France.
[Ti] Título:Rapid, cost-effective and accurate quantification of Yucca schidigera Roezl. steroidal saponins using HPLC-ELSD method.
[So] Source:Food Chem;221:1245-1252, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Yucca GRAS-labelled saponins have been and are increasingly used in food/feed, pharmaceutical or cosmetic industries. Existing techniques presently used for Yucca steroidal saponin quantification remain either inaccurate and misleading or accurate but time consuming and cost prohibitive. The method reported here addresses all of the above challenges. HPLC/ELSD technique is an accurate and reliable method that yields results of appropriate repeatability and reproducibility. This method does not over- or under-estimate levels of steroidal saponins. HPLC/ELSD method does not require each and every pure standard of saponins, to quantify the group of steroidal saponins. The method is a time- and cost-effective technique that is suitable for routine industrial analyses. HPLC/ELSD methods yield a saponin fingerprints specific to the plant species. As the method is capable of distinguishing saponin profiles from taxonomically distant species, it can unravel plant adulteration issues.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Saponinas/análise
Yucca/química
[Mh] Termos MeSH secundário: Análise Custo-Benefício
Extratos Vegetais/análise
Espalhamento de Radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Saponins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  4 / 111 MEDLINE  
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[PMID]:27746005
[Au] Autor:Vlcková R; Sopková D; Andrejcáková Z; Valocký I; Kádasi A; Harrath AH; Petrilla V; Sirotkin AV
[Ad] Endereço:Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic. Electronic address: radkavl@seznam.cz.
[Ti] Título:Dietary supplementation of yucca (Yucca schidigera) affects ovine ovarian functions.
[So] Source:Theriogenology;88:158-165, 2017 Jan 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yucca (Yucca schidigera) is a popular medicinal plant due to its many positive effects on animal and human physiology, including their reproductive systems. To examine the effect of supplemental yucca feeding on sheep reproduction, including ovarian functions and their hormonal regulators, ewes were fed (or not fed, control) yucca powder (1.5 g/head/day, 30 days). Macromorphometric indexes of the oviduct, ovary, and ovarian folliculogenesis were measured. Reproductive hormone levels in the blood were measured using a radioimmunoassay. Granulosa cells were aspirated from the ovary, and their proliferation and apoptosis were detected using immunocytochemistry. To assess secretory activity and its response to gonadotropin, ovarian fragments of treated and control ewes were cultured with and without follicle-stimulating hormone (FSH; 0, 0.1, 1, 10, or 100 IU/mL), and the release of reproductive hormones into the culture medium was evaluated. Finally, to examine the direct action of yucca on the ovary, ovarian fragments from control ewes were cultured with and without yucca extract (1, 10, or 100 µg/mL), and the release of reproductive hormones was measured. Yucca supplementation significantly decreased the size of small antral follicles (2 to <5 mm in diameter), increased accumulation of the apoptosis marker bax, and decreased serum progesterone (P4) and estradiol (E2) levels. It inhibited the release of P4 (but not other hormones), to prevent the stimulatory action of FSH on P4 output and promoted insulin-like growth factor I (IGF-I) release by fragments cultured with FSH. However, yucca supplementation did not affect the size of larger follicles and number of follicles, volume and weight of ovaries, length and weight of oviducts, caspase 3 accumulation, cell proliferation, testosterone (T) or IGF-I serum levels, or T or E2 release by cultured ovarian fragments and their response to FSH. Yucca addition to culture medium inhibited P4 and IGF-I, but not T or E2 release at the lowest (1 µg/mL) dose, and stimulated P4, but not T, E2, or IGF-I release at the highest (100 µg/mL) dose. These data suggest that yucca supplementation can reduce small antral ovarian follicle development possibly via the stimulation of apoptosis of their granulosa cells, suppression of ovarian P4 and E2 release, and alteration of ovarian IGF-I output and ovarian response to gonadotropin. Thus, yucca can directly affect P4 and IGF-I release by ovine ovarian cells.
[Mh] Termos MeSH primário: Ração Animal/análise
Dieta/veterinária
Ovário/fisiologia
Ovinos/fisiologia
Yucca
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Suplementos Nutricionais
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27793858
[Au] Autor:McKain MR; McNeal JR; Kellar PR; Eguiarte LE; Pires JC; Leebens-Mack J
[Ad] Endereço:Donald Danforth Plant Science Center, 975 N. Warson Road, St. Louis, Missouri 63132 USA mrmckain@gmail.com.
[Ti] Título:Timing of rapid diversification and convergent origins of active pollination within Agavoideae (Asparagaceae).
[So] Source:Am J Bot;103(10):1717-1729, 2016 Oct.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Yucca species are ideal candidates for the study of coevolution due to the obligate mutualism they form with yucca moth pollinators (genera Tegeticula and Parategeticula). Yuccas are not the only species to exhibit a mutualism with yucca moths; the genus Hesperoyucca is pollinated by the California yucca moth (Tegeticula maculata). Relationships among yuccas, Hesperoyucca, and other members of subfamily Agavoideae are necessary to understand the evolution of this unique pollination syndrome. Here, we investigate evolutionary relationships of yuccas and closely related genera looking at the timing and origin of yucca moth pollination. METHODS: In this study, we sequenced the chloroplast genomes of 20 species in the subfamily Agavoideae (Asparagaceae) and three confamilial outgroup taxa to resolve intergeneric phylogenetic relationships of Agavoideae. We estimated divergence times using protein-coding genes from 67 chloroplast genomes sampled across monocots to determine the timing of the yucca moth pollination origin. KEY RESULTS: We confidently resolved intergeneric relationships in Agavoideae, demonstrating the origin of the yucca-yucca moth mutualism on two distinct lineages that diverged 27 million years ago. Comparisons of Yucca and Hesperoyucca divergence time to those of yucca moths (Tegeticula and Parategeticula, Prodoxidae) indicate overlapping ages for the origin of pollinating behavior in the moths and pollination by yucca moths in the two plant lineages. CONCLUSION: Whereas pollinating yucca moths have been shown to have a single origin within the Prodoxidae, there were independent acquisitions of active pollination on lineages leading to Yucca and Hesperoyucca within the Agavoideae.
[Mh] Termos MeSH primário: Asparagaceae/fisiologia
Biodiversidade
Evolução Biológica
Genoma de Cloroplastos/genética
Mariposas/fisiologia
Polinização
Simbiose
[Mh] Termos MeSH secundário: Animais
Asparagaceae/genética
Evolução Molecular
Sequenciamento de Nucleotídeos em Larga Escala
Filogenia
Análise de Sequência de DNA
Yucca/genética
Yucca/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:27671531
[Au] Autor:Royer AM; Streisfeld MA; Smith CI
[Ad] Endereço:Department of Biology, Willamette University, Salem, Oregon 97301 USA aroyer@willamette.edu.
[Ti] Título:Population genomics of divergence within an obligate pollination mutualism: Selection maintains differences between Joshua tree species.
[So] Source:Am J Bot;103(10):1730-1741, 2016 Oct.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Speciation is a complex process that can be shaped by many factors, from geographic isolation to interspecific interactions. In Joshua trees, selection from pollinators on style length has been hypothesized to contribute to the maintenance of differentiation between two hybridizing sister species. We used population genomics approaches to measure the extent of genetic differentiation between these species, test whether selection maintains differences between them, and determine whether genetic variants associated with style length show signatures of selection. METHODS: Using restriction-site-associated DNA (RAD)-sequencing, we identified 9516 single nucleotide polymorphisms (SNPs) across the Joshua tree genome. We characterized the genomic composition of trees in a narrow hybrid zone and used genomic scans to search for signatures of selection acting on these SNPs. We used a genome-wide association study to identify SNPs associated with variation in phenotypic traits, including style length, and asked whether those SNPs were overrepresented among the group under selection. KEY RESULTS: The two species were highly genetically differentiated (F = 0.25), and hybrids were relatively rare in the hybrid zone. Approximately 20% of SNPs showed evidence of selection maintaining divergence. While SNPs associated with style length were overrepresented among those under selection (P << 0.0001), the same was true for SNPs associated with highly differentiated vegetative traits. CONCLUSIONS: The two species of Joshua tree are clearly genetically distinct, and selection is maintaining differences between them. We found that loci associated with differentiated traits were likely to be under selection. However, many traits other than style length appeared to be under selection. Together with the dearth of intermediate hybrids, these findings reveal that these taxa are more strongly diverged than previously suspected and that selection, likely on many targets, is maintaining separation where the two species meet and hybridize.
[Mh] Termos MeSH primário: Genoma de Planta
Metagenômica
Mariposas/fisiologia
Polinização
Polimorfismo de Nucleotídeo Único
Simbiose
Yucca/fisiologia
[Mh] Termos MeSH secundário: Animais
Estudo de Associação Genômica Ampla
Nevada
Seleção Genética
Análise de Sequência de DNA
Especificidade da Espécie
Yucca/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160928
[St] Status:MEDLINE


  7 / 111 MEDLINE  
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[PMID]:27578627
[Au] Autor:Svensson GP; Raguso RA; Flatz R; Smith CI
[Ad] Endereço:Department of Biology, Lund University, Solvegatan 37, SE-22362 Lund Sweden glenn.svensson@biol.lu.se.
[Ti] Título:Floral scent of Joshua trees (Yucca brevifolia sensu lato): Divergence in scent profiles between species but breakdown of signal integrity in a narrow hybrid zone.
[So] Source:Am J Bot;103(10):1793-1802, 2016 Oct.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: The role of floral scent in facilitating reproductive isolation between closely related plants remains poorly understood. Yucca brevifolia and Yucca jaegeriana are pollinated by different moth species in allopatry, but in a narrow contact zone, pollinator-host specificity breaks down, resulting in hybridization between species. We explored the chemical basis for reproductive isolation and hybridization in these Joshua trees by characterizing the floral scent of each species in allopatry, analyzing scent profiles from trees in the contact zone, and matching these data with genotypic and phenotypic data. METHODS: We analyzed floral volatiles using gas chromatography-mass spectrometry, tested for species divergence of scent profiles and classified trees in the contact zone as hybrid or either parental species. We used floral and vegetative morphological data and genotypic data to classify trees and analyzed whether certain trait combinations were more correlated than others with respect to assignment of trees and whether frequencies of classified tree types differed depending on which data set was used. KEY RESULTS: The Joshua tree floral scent included oxygenated 8-carbon compounds not reported for other yuccas. The two species differed (P < 0.001) in scent profiles. In the contact zone, many hybrids were found, and phenotypic traits were generally weakly correlated, which may be explained by extensive gene flow between species or by exposure to different selection pressures. CONCLUSIONS: Although the two Joshua tree species produce distinct floral scent profiles, it is insufficient to prevent attraction of associated pollinators to both hosts. Instead, floral morphology may be the key trait mediating gene flow between species.
[Mh] Termos MeSH primário: Hibridização Genética
Isolamento Reprodutivo
Yucca/genética
[Mh] Termos MeSH secundário: Flores/anatomia & histologia
Flores/química
Flores/fisiologia
Repetições de Microssatélites
Nevada
Odorantes/análise
Feromônios/análise
Yucca/anatomia & histologia
Yucca/química
Yucca/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE


  8 / 111 MEDLINE  
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[PMID]:27555437
[Au] Autor:Althoff DM
[Ad] Endereço:Department of Biology, 107 College Place, Syracuse University, Syracuse, New York 13244 USA dmalthof@syr.edu.
[Ti] Título:Specialization in the yucca-yucca moth obligate pollination mutualism: A role for antagonism?
[So] Source:Am J Bot;103(10):1803-1809, 2016 Oct.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Specialized brood pollination systems involve both mutualism and antagonism in the overall interaction and have led to diversification in both plants and insects. Although largely known for mutualism, the role of the antagonistic side of the interaction in these systems has been overlooked. Specialization may be driven by plant defenses to feeding by the insect larvae that consume and kill developing plant ovules. The interaction among yuccas and yucca moths is cited as a classic example of the importance of mutualism in specialization and diversification. Pollinators moths are very host specific, but whether this specificity is due to adult pollination ability or larval feeding ability is unclear. Here, I test the potential role of antagonism in driving specialization among yuccas and yucca moths. METHODS: I examined the ability of the most-polyphagous yucca moth pollinator, Tegeticula yuccasella, to pollinate and develop on five Yucca species used across its range. Yucca species endemic to the Great Plains and Texas were transplanted to a common garden in Syracuse, New York and exposed to the local pollinator moth population over 3 years. KEY RESULTS: Local moths visited all but one of the Yucca species, but had drastically lower rates of successful larval development on non-natal Yucca species in comparison to the local host species. CONCLUSION: Specialization in many brood pollination systems may be strongly influenced by the antagonistic rather than the mutualistic side of the overall interaction, suggesting that antagonistic coevolution is a possible source of diversification.
[Mh] Termos MeSH primário: Mariposas/fisiologia
Polinização
Simbiose
Yucca/fisiologia
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Larva/crescimento & desenvolvimento
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
Oviposição
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE


  9 / 111 MEDLINE  
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[PMID]:27208351
[Au] Autor:Borchert MI; DeFalco LA
[Ad] Endereço:San Bernardino National Forest, P.O. Box 292, Fawnskin, California 92333 borchert1@charter.net.
[Ti] Título:Yucca brevifolia fruit production, predispersal seed predation, and fruit removal by rodents during two years of contrasting reproduction.
[So] Source:Am J Bot;103(5):830-6, 2016 05.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: The distribution of Yucca brevifolia, a keystone species of the Mojave Desert, may contract with climate change, yet reproduction and dispersal are poorly understood. We tracked reproduction, seed predation, and fruit dispersal for two years and discuss whether Y. brevifolia is a masting species. METHODS: Fruit maturation, seed predation (larval yucca moths), and fruit dispersal (rodents) were monitored on a random sample of panicles during 2013 and 2014, which were years of high and low reproduction, respectively. Fates of fruits placed on the ground and in canopies were also tracked. Rodents were live-trapped to assess abundance and species composition. KEY RESULTS: In 2013, 66% of inflorescences produced fruit of which 53% escaped larval predation; 19.5% of seeds were destroyed in infested fruits. Total seed production was estimated to be >100 times greater in 2013 than 2014. One-third of the fruit crop fell to the ground and was removed by rodents over the course of 120 d. After ground fruits became scarce, rodents exploited canopy fruits. Rodent numbers were low in 2013, so fruits remained in canopies for 370 d. In 2014, fruit production was approximately 20% lower. Larvae infested the majority of fruits, and almost twice the number of seeds were damaged. Fruits were exploited by rodents within 65 d. CONCLUSIONS: High fertilization, prolific seed production, and low predispersal predation in 2013 suggests that pollinator attraction and satiation of seed predators influence masting in Y. brevifolia. Abundant, prolonged fruit availability to seed-dispersing rodents likely extends recruitment opportunities during mast years.
[Mh] Termos MeSH primário: Frutas/crescimento & desenvolvimento
Comportamento Predatório/fisiologia
Dispersão de Sementes/fisiologia
Yucca/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Inflorescência/fisiologia
Larva/fisiologia
Reprodução
Roedores
Sementes/fisiologia
Especificidade da Espécie
Yucca/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1500516


  10 / 111 MEDLINE  
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[PMID]:27107534
[Au] Autor:Nakashima K; Abe N; Oyama M; Inoue M
[Ad] Endereço:Laboratory of Medicinal Resources, School of Pharmacy, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. Electronic address: nakasima@dpc.agu.ac.jp.
[Ti] Título:Yuccalides A-C, three new phenolic compounds with spiro-structures from the roots of Yucca gloriosa.
[So] Source:Fitoterapia;111:154-9, 2016 Jun.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three new phenolic compounds, yuccalides A-C (1-3), were isolated from the roots of Yucca gloriosa L., along with four known compounds (4-7). The structures of the new compounds were established by extensive NMR spectroscopic analyses. Inducible nitric oxide synthase (iNOS) mRNA levels induced by lipopolysaccharide (LPS) in mouse macrophage-like RAW 264.7 cells were effectively suppressed by compounds 2, 4, and 6, all of which had the (2R*, 3R*)-configuration. IL-1ß and IL-6 mRNA levels induced by LPS were significantly attenuated by compounds 4, 5, and 6, but not by 2.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Fenóis/química
Raízes de Plantas/química
Yucca/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Interleucina-1beta/metabolismo
Interleucina-6/metabolismo
Camundongos
Estrutura Molecular
Óxido Nítrico Sintase Tipo II/metabolismo
Fenóis/isolamento & purificação
Células RAW 264.7
Compostos de Espiro/química
Compostos de Espiro/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Phenols); 0 (Spiro Compounds); 0 (yuccalide B); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160425
[St] Status:MEDLINE



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