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Pesquisa : B01.650.940.800.575.912.250.618.100.640 [Categoria DeCS]
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  1 / 1447 MEDLINE  
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[PMID]:29176771
[Au] Autor:Zhang W; Gao J
[Ad] Endereço:Centre for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan, China.
[Ti] Título:Multiple factors contribute to reproductive isolation between two co-existing Habenaria species (Orchidaceae).
[So] Source:PLoS One;12(11):e0188594, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reproductive isolation is a key feature that forms barriers to gene flow between distinct plants. In orchids, prezygotic reproductive isolation has been considered to be strong, because their associations with highly specific pollinators. In this study, the reproductive ecology and reproductive isolation of two sympatric Habenaria species, H. davidii and H. fordii, was investigated by floral phenology and morphology, hand-pollination experiments and visitor observation in southwest China. The two species were dependent on insects for pollination and completely self-compatible. A number of factors have been identified to limit gene flow between the two species and achieved full reproductive isolation. Ecogeographic isolation was a weak barrier. H. fordii and H. davidii had completely overlapped flowering periods, and floral morphology plays an important role in floral isolation. The two species shared the same hawkmoth pollinator, Cechenena lineosa, but the pollinaria of the two orchids were attached on different body parts of pollinators. Prezygotic isolation was not complete, but the interspecific pollination treatments of each species resulted in no seed sets, indicating that unlike many other orchid species, in which the postzygotic reproductive isolation is very weak or complete absence, the post-zygotic isolation strongly acted in the stage of seed production between two species. The results illustrate the reproductive isolation between two species involves multiple plant life-history stages and a variety of reproductive barriers can contribute to overall isolation.
[Mh] Termos MeSH primário: Orchidaceae/fisiologia
Isolamento Reprodutivo
[Mh] Termos MeSH secundário: Animais
Ecossistema
Flores/anatomia & histologia
Frutas/fisiologia
Geografia
Inflorescência/anatomia & histologia
Insetos
Microscopia de Fluorescência
Tubo Polínico/crescimento & desenvolvimento
Polinização
Simpatria
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188594


  2 / 1447 MEDLINE  
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[PMID]:28797947
[Au] Autor:Ong JWL; Li H; Sivasithamparam K; Dixon KW; Jones MGK; Wylie SJ
[Ad] Endereço:Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
[Ti] Título:The challenges of using high-throughput sequencing to track multiple bipartite mycoviruses of wild orchid-fungus partnerships over consecutive years.
[So] Source:Virology;510:297-304, 2017 Oct.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bipartite alpha- and betapartitiviruses are recorded from a wide range of fungi and plants. Using a combination of dsRNA-enrichment, high-throughput shotgun sequencing and informatics, we report the occurrence of multiple new partitiviruses associated with mycorrhizal Ceratobasidium fungi, themselves symbiotically associated with a small wild population of Pterostylis sanguinea orchids in Australia, over two consecutive years. Twenty-one partial or near-complete sequences representing 16 definitive alpha- and betapartitivirus species, and further possible species, were detected from two fungal isolates. The majority of partitiviruses occurred in fungal isolates from both years. Two of the partitiviruses represent phylogenetically divergent forms of Alphapartitivirus, suggesting that they may have evolved under long geographical isolation there. We address the challenge of pairing the two genomic segments of partitiviruses to identify species when multiple partitiviruses co-infect a single host.
[Mh] Termos MeSH primário: Basidiomycota/virologia
Micovírus/classificação
Micovírus/isolamento & purificação
Orchidaceae/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Austrália
Biologia Computacional
Micovírus/genética
Sequenciamento de Nucleotídeos em Larga Escala
Estudos Longitudinais
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE


  3 / 1447 MEDLINE  
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[PMID]:28764749
[Au] Autor:Bhatnagar M; Sarkar N; Gandharv N; Apang O; Singh S; Ghosal S
[Ad] Endereço:Center for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, 201303, India.
[Ti] Título:Evaluation of antimycobacterial, leishmanicidal and antibacterial activity of three medicinal orchids of Arunachal Pradesh, India.
[So] Source:BMC Complement Altern Med;17(1):379, 2017 Aug 01.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The ethnic population of Arunachal Pradesh uses a number of orchids as such, or in decoction for various ailments. Three untapped orchids namely, Rhynchostylis retusa, Tropidia curculioides and Satyrium nepalense, traditionally used in tuberculosis, asthma and cold stage of malaria in folk medicine, were selected for the present study. METHODS: Dried material of each plant was divided into three parts. Solvent extraction and fractionation afforded altogether 30 extracts and fractions, which were evaluated against Mycobacterium tuberculosis (H37Rv and MDR strain) for antimycobacterial activity; promastigotes and amastigotes of Leishmania donovani for leishmanicidal activity and two gram positive and three gram negative clinical isolates for antibacterial activity. RESULTS: The most significant antimycobacterial activity was observed with n-hexane fraction of the flower of Satyrium nepalense with MIC of 15.7 µg/mL. The most promising leishmanicidal activity was observed with diethyl ether fraction of the roots of Rhynchostylis retusa with IC values of 56.04 and 18.4 µg/mL against promastigotes and intracellular amastigotes respectively. Evaluation of antibacterial activity identified S. nepalense flower n-hexane and R. retusa roots diethyl ether as potential fractions with MIC values of ≤100 µg/mL against selected clinical isolates. CONCLUSIONS: This is the first report of the plants possessing antimycobacterial and leishmanicidal activity. The investigation resulted in identification of S. nepalense as the most promising plant, which possessed all three activities in significant proportion. This laboratory outcome could be translated to marketable pharmaceutical products and also to produce maximum benefits to the local of nearby area. Antimycobacterial and leishmanicidal activity of medicinal orchids.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Leishmania donovani/efeitos dos fármacos
Mycobacterium tuberculosis/efeitos dos fármacos
Orchidaceae
Extratos Vegetais/farmacologia
Tripanossomicidas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antituberculosos/farmacologia
Linhagem Celular
Flores
Índia
Medicina Tradicional
Camundongos
Testes de Sensibilidade Microbiana
Raízes de Plantas
Plantas Medicinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antitubercular Agents); 0 (Plant Extracts); 0 (Trypanocidal Agents)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1884-z


  4 / 1447 MEDLINE  
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[PMID]:28704518
[Au] Autor:Zheng BQ; Zou LH; Li K; Wan X; Wang Y
[Ad] Endereço:State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
[Ti] Título:Photosynthetic, morphological, and reproductive variations in Cypripedium tibeticum in relation to different light regimes in a subalpine forest.
[So] Source:PLoS One;12(7):e0181274, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cypripedium tibeticum, a subalpine orchid species, inhabits various habitats of subalpine forests, mainly including the forest edge (FE), forest gap (FG), and understory (UST), which have significantly different light intensities (FE > FG > UST). However, the ecological and physiological influences caused by different light regimes in this species are still poorly understood. In the present study, photosynthetic, morphological, and reproductive characteristics were comprehensively studied in plants of C. tibeticum grown in three types of habitats. The photosynthetic capacities, such as the net photosynthetic rate, light-saturated photosynthesis (Pmax), and dry mass per unit leaf area (LMA), were higher in FE and FG than in UST according to light availability. Compared with FG, the populations in FE and UST suffer from excessively strong and inadequate radiation, respectively, which was further corroborated by the low Fv/Fm in FE and high apparent quantum yield (AQY) in FG. The leaves of the orchids had various proportions of constituents, such as the leaf area, thickness and (or) epidermal hair, to reduce damage from high radiation (including ultraviolet-b radiation) in FE and capture more light in FG and UST. Although the flower rate (FR) was positively correlated to both Pmax and the daily mean PAR, fruit-set only occurred in the populations in FG. The failures in FE and UST might be ascribed to changes in the floral functional structure and low biomass accumulation, respectively. Moreover, analysis of the demographic statistics showed that FG was an advantageous habitat for the orchid. Thus, C. tibeticum reacted to photosynthetic and morphological changes to adapt to different subalpine forest habitats, and neither full (under FE) nor low (UST) illumination was favorable for population expansion. These findings could serve as a guide for the protection and reintroduction of C. tibeticum and emphasize the importance of specific habitats for Cypripedium spp.
[Mh] Termos MeSH primário: Aclimatação
Variação Genética
Orchidaceae/genética
Fotossíntese
Luz Solar
[Mh] Termos MeSH secundário: Altitude
Biomassa
Flores/genética
Flores/crescimento & desenvolvimento
Florestas
Orchidaceae/crescimento & desenvolvimento
Orchidaceae/metabolismo
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181274


  5 / 1447 MEDLINE  
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[PMID]:28685577
[Au] Autor:Lin L; Zheng F; Zhou H; Li S
[Ad] Endereço:Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology and §College of Chemistry and Environmental Science, Minnan Normal University , Zhangzhou, Fujian 363000, People's Republic of China.
[Ti] Título:Biomimetic Gastrointestinal Tract Functions for Metal Absorption Assessment in Edible Plants: Comparison to In Vivo Absorption.
[So] Source:J Agric Food Chem;65(30):6282-6287, 2017 Aug 02.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A biomimetic gastrointestinal tract, including in vitro digestion and biomimetic biomembrane extraction, has been proposed for absorption assessment of metals from edible plants. However, its validity is still unknown. Herein, two species of edible plants, Anoectochilus roxburghii and Radix astragali, were selected and digested in a bionic mouth, stomach, and intestine, and then trace metals (Cr, Mn, Fe, Ni, Cu, Zn, Se, Sr, As, and Pb) were transformed to their final metal species. To check model predictability, in vitro and in vivo metal absorption were imitated and tested by monolayer liposome extraction and rat stomach or single-pass duodenal intestine, respectively. A strong correlation was established between in vivo and in vitro metal absorption ratios, with 0.89 > R > 0.66, and a significant relationship (p < 0.05) was exhibited for stomach, intestine, two plant species, and 10 metal species. Our biomimetic system could be used as low-cost alternatives to animal and clinical studies for multi-metal absorption.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/metabolismo
Trato Gastrointestinal/metabolismo
Metais/metabolismo
Orchidaceae/metabolismo
Plantas Comestíveis/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomimética
Digestão
Medicamentos de Ervas Chinesas/química
Trato Gastrointestinal/química
Seres Humanos
Absorção Intestinal
Metais/análise
Orchidaceae/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Metals); 922OP8YUPF (Huang Qi)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02054


  6 / 1447 MEDLINE  
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[PMID]:28541415
[Au] Autor:Valoroso MC; De Paolo S; Iazzetti G; Aceto S
[Ad] Endereço:Department of Biology, University of Naples Federico II, Naples, Italy.
[Ti] Título:Transcriptome-Wide Identification and Expression Analysis of DIVARICATA- and RADIALIS-Like Genes of the Mediterranean Orchid Orchis italica.
[So] Source:Genome Biol Evol;9(6), 2017 Jun 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bilateral symmetry of flowers is a relevant novelty that has occurred many times throughout the evolution of flowering plants. In Antirrhinum majus, establishment of flower dorso-ventral asymmetry is mainly due to interaction of TCP (CYC and DICH) and MYB (DIV, RAD, and DRIF) transcription factors. In the present study, we characterized 8 DIV-, 4 RAD-, and 2 DRIF-like genes from the transcriptome of Orchis italica, an orchid species with bilaterally symmetric and resupinate flowers. We found a similar number of DIV- and RAD-like genes within the genomes of Phalaenopsis equestris and Dendrobium catenatum orchids. Orchid DIV- and RAD-like proteins share conserved motifs whose distribution reflects their phylogeny and analysis of the genomic organization revealed a single intron containing many traces of transposable elements. Evolutionary analysis has shown that purifying selection acts on the DIV- and RAD-like coding regions in orchids, with relaxation of selective constraints in a branch of the DIV-like genes. Analysis of the expression patterns of DIV- and RAD-like genes in O. italica revealed possible redundant functions for some of them. In the perianth of O. italica, the ortholog of DIV and DRIF of A. majus are expressed in all tissues, whereas RAD is mainly expressed in the outer tepals and lip. These data allow for proposal of an evolutionary conserved model in which the expression of the orthologs of the DIV, RAD, and DRIF genes might be related to establishment of flower bilateral symmetry in the nonmodel orchid species O. italica.
[Mh] Termos MeSH primário: Flores/genética
Regulação da Expressão Gênica de Plantas
Orchidaceae/genética
Proteínas de Plantas/genética
Fatores de Transcrição/genética
Transcriptoma
[Mh] Termos MeSH secundário: Evolução Molecular
Orchidaceae/crescimento & desenvolvimento
Fenótipo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx101


  7 / 1447 MEDLINE  
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[PMID]:28536987
[Au] Autor:Cuervo M; Rakosy D; Martel C; Schulz S; Ayasse M
[Ad] Endereço:Institute of Evolutionary Ecology and Conservation Genomics, Ulm University, Helmholtzstraße 10-1, Containerstadt, 89081, Ulm, Germany. monica.cuervo@uni-ulm.de.
[Ti] Título:Sexual Deception in the Eucera-Pollinated Ophrys leochroma: A Chemical Intermediate between Wasp- and Andrena-Pollinated Species.
[So] Source:J Chem Ecol;43(5):469-479, 2017 May.
[Is] ISSN:1573-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ophrys flowers mimic sex pheromones of attractive females of their pollinators and attract males, which attempt to copulate with the flower and thereby pollinate it. Virgin females and orchid flowers are known to use the same chemical compounds in order to attract males. The composition of the sex pheromone and its floral analogue, however, vary between pollinator genera. Wasp-pollinated Ophrys species attract their pollinators by using polar hydroxy acids, whereas Andrena-pollinated species use a mixture of non-polar hydrocarbons. The phylogeny of Ophrys shows that its evolution was marked by episodes of rapid diversification coinciding with shifts to different pollinator groups: from wasps to Eucera and consequently to Andrena and other bees. To gain further insights, we studied pollinator attraction in O. leochroma in the context of intra- and inter-generic pollinator shifts, radiation, and diversification in the genus Ophrys. Our model species, O. leochroma, is pollinated by Eucera kullenbergi males and lies in the phylogeny between the wasp and Andrena-pollinated species; therefore, it is a remarkable point to understand pollinator shifts. We collected surface extracts of attractive E. kullenbergi females and labellum extracts of O. leochroma and analyzed them by using gas chromatography with electroantennographic detection (GC-EAD) and gas chromatography coupled with mass spectrometry (GC-MS). We also performed field bioassays. Our results show that O. leochroma mimics the sex pheromone of its pollinator's female by using aldehydes, alcohols, fatty acids, and non-polar compounds (hydrocarbons). Therefore, in terms of the chemistry of pollinator attraction, Eucera-pollinated Ophrys species might represent an intermediate stage between wasp- and Andrena-pollinated orchid species.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Orchidaceae/química
Pólen/química
Vespas/fisiologia
[Mh] Termos MeSH secundário: Animais
Cromatografia Gasosa
Decepção
Feminino
Flores/química
Flores/metabolismo
Cromatografia Gasosa-Espectrometria de Massas
Masculino
Orchidaceae/classificação
Orchidaceae/metabolismo
Filogenia
Pólen/metabolismo
Atrativos Sexuais/análise
Atrativos Sexuais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sex Attractants)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-017-0848-6


  8 / 1447 MEDLINE  
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[PMID]:28532402
[Au] Autor:Shi Y; Zhang B; Lu Y; Qian C; Feng Y; Fang L; Ding Z; Cheng D
[Ad] Endereço:College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China.
[Ti] Título:Antiviral activity of phenanthrenes from the medicinal plant Bletilla striata against influenza A virus.
[So] Source:BMC Complement Altern Med;17(1):273, 2017 May 22.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Influenza represents a serious public health concern. The emergence of resistance to anti-influenza drugs underlines the need to develop new drugs. This study aimed to evaluate the anti-influenza viral activity and possible mechanisms of 12 phenanthrenes from the medicinal plant Bletilla striata (Orchidaceae family). METHODS: Twelve phenanthrenes were isolated and identified from B. striata. Influenza virus A/Sydney/5/97 (H3N2) propagated in embryonated chicken eggs was used. Phenanthrenes mixed with the virus were incubated at 37 °C for 1 h and then inoculated into 9-day-old embryonated chicken eggs via the allantoic route to survey the antiviral activity in vivo. A (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS)-based assay was performed to evaluate the reduction of cytopathic effect induced by H3N2 on Madin-Darby canine kidney (MDCK) cells. The hemagglutination inhibition assay was used to study the blockage of virus receptors by the phenanthrenes, and the neuraminidase (NA) inhibition assay to evaluate the effects of the release of virus. The synthesis of influenza viral matrix protein mRNA in response to compound treatment was measured by real-time polymerase chain reaction. RESULTS: This study showed that phenanthrenes 1, 2, 3, 4, 6, 9, 10, 11, and 12 significantly inhibited the viruses in vivo, with inhibition rates of 20.7, 79.3, 17.2, 34.5, 34.5, 34.5, 44.8, 75.9, and 34.5%, respectively. In MDCK models, the phenanthrenes did not show significant antiviral activity when administered as pretreatment, while phenanthrenes 2, 3, 4, 6, 7 10, and 11 exhibited inhibitory activities as simultaneous treatment with 50% inhibition concentration (IC ) ranging from 14.6 ± 2.4 to 43.3 ± 5.3 µM. The IC ranged from 18.4 ± 3.1 to 42.3 ± 3.9 µM in the post-treatment assays. Compounds 1, 3, 4, 6, 10, and 11 exhibited an inhibitory effect on NA; and compounds 2, 3, 4 6, 7, 10, and 11 resulted in the reduced transcription of virus matrix protein mRNA. However, no compound could inhibit hemagglutination by the influenza virus. CONCLUSION: Phenanthrenes from B. striata had strong anti-influenza viral activity in both embryonated eggs and MDCK models, and diphenanthrenes seemed to have stronger inhibition activity compared with monophenanthrenes.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Vírus da Influenza A Subtipo H3N2/efeitos dos fármacos
Influenza Humana/virologia
Orchidaceae/química
Fenantrenos/farmacologia
Extratos Vegetais/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Antivirais/química
Antivirais/isolamento & purificação
Seres Humanos
Vírus da Influenza A Subtipo H3N2/fisiologia
Influenza Humana/tratamento farmacológico
Fenantrenos/química
Fenantrenos/isolamento & purificação
Extratos Vegetais/química
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Phenanthrenes); 0 (Plant Extracts)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1780-6


  9 / 1447 MEDLINE  
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[PMID]:28478311
[Au] Autor:Kondo H; Hirota K; Maruyama K; Andika IB; Suzuki N
[Ad] Endereço:Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan. Electronic address: hkondo@okayama-u.ac.jp.
[Ti] Título:A possible occurrence of genome reassortment among bipartite rhabdoviruses.
[So] Source:Virology;508:18-25, 2017 Aug.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Orchid fleck virus (OFV) represents a rhabdovirus with a unique bipartite genome. OFV genetic diversity at the whole genome level has not been described. Using the partial genome sequence of RNA1, we have determined that several OFV isolates derived from orchids in Japan belong to two genetically distant subgroups: subgroup I, the members of which are distributed worldwide but previously not known in Asia, and subgroup II, which is commonly distributed in Japan. However, complete genome sequence analysis of a novel Japanese subgroup I isolate revealed that although its RNA1 sequence differs considerably from those of subgroup II isolates, its RNA2 sequence is almost identical to them. Based on phylogenetic and recombination analyses, the genome reassortment events were predicted to occur between OFV subgroups including other unseen strains. Our data show that genome reassortment contributes to the genetic diversities of the bipartite rhabdoviruses and its occurrence may be geographically constrained.
[Mh] Termos MeSH primário: Genoma Viral
Orchidaceae/virologia
Doenças das Plantas/virologia
Vírus Reordenados/genética
Rhabdoviridae/genética
[Mh] Termos MeSH secundário: Ásia
Fases de Leitura Aberta
Filogenia
Vírus Reordenados/classificação
Vírus Reordenados/fisiologia
Recombinação Genética
Rhabdoviridae/classificação
Rhabdoviridae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE


  10 / 1447 MEDLINE  
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[PMID]:28411481
[Au] Autor:Yang H; Barros-Rios J; Kourteva G; Rao X; Chen F; Shen H; Liu C; Podstolski A; Belanger F; Havkin-Frenkel D; Dixon RA
[Ad] Endereço:BioDiscovery Institute, University of North Texas, Denton, TX 76203, USA.
[Ti] Título:A re-evaluation of the final step of vanillin biosynthesis in the orchid Vanilla planifolia.
[So] Source:Phytochemistry;139:33-46, 2017 Jul.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A recent publication describes an enzyme from the vanilla orchid Vanilla planifolia with the ability to convert ferulic acid directly to vanillin. The authors propose that this represents the final step in the biosynthesis of vanillin, which is then converted to its storage form, glucovanillin, by glycosylation. The existence of such a "vanillin synthase" could enable biotechnological production of vanillin from ferulic acid using a "natural" vanilla enzyme. The proposed vanillin synthase exhibits high identity to cysteine proteases, and is identical at the protein sequence level to a protein identified in 2003 as being associated with the conversion of 4-coumaric acid to 4-hydroxybenzaldehyde. We here demonstrate that the recombinant cysteine protease-like protein, whether expressed in an in vitro transcription-translation system, E. coli, yeast, or plants, is unable to convert ferulic acid to vanillin. Rather, the protein is a component of an enzyme complex that preferentially converts 4-coumaric acid to 4-hydroxybenzaldehyde, as demonstrated by the purification of this complex and peptide sequencing. Furthermore, RNA sequencing provides evidence that this protein is expressed in many tissues of V. planifolia irrespective of whether or not they produce vanillin. On the basis of our results, V. planifolia does not appear to contain a cysteine protease-like "vanillin synthase" that can, by itself, directly convert ferulic acid to vanillin. The pathway to vanillin in V. planifolia is yet to be conclusively determined.
[Mh] Termos MeSH primário: Benzaldeídos/metabolismo
Cisteína Proteases/metabolismo
Orchidaceae/química
Vanilla/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ácidos Cumáricos/metabolismo
Escherichia coli
Propionatos
Vanilla/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzaldehydes); 0 (Coumaric Acids); 0 (Propionates); AVM951ZWST (ferulic acid); CHI530446X (vanillin); EC 3.4.- (Cysteine Proteases); H81U1KBS6E (glucovanillin); IBS9D1EU3J (trans-3-(4'-hydroxyphenyl)-2-propenoic acid); O1738X3Y38 (4-hydroxybenzaldehyde)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE



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