Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.618.100.640.500 [Categoria DeCS] Referências encontradas : 177 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 29305972 [Au] Autor: Zeng X; Ling H; Yang J; Li Y; Guo S [Ad] Endereço: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, PR China. [Ti] Título: LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli. [So] Source: Gene;646:136-142, 2018 Mar 10. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: BACKGROUND: Late embryogenesis abundant (LEA) proteins were initially discovered about 35years ago as accumulating late in embryogenesis of cotton seeds. Although abundant in seeds and pollens, these proteins have been found to protect cells against desiccation, cold, high temperature, and high salinity. OBJECTIVE: Here, we present the first comprehensive survey of LEA proteins and their encoding genes in Gastrodia elata, a well-known medicinal orchid in China. Moreover, we researched on LEA family evolutionary relationships and functional characteristics. METHODS: The LEA gene family in G. elata (GeLEAs) was cloned based on RNA-Seq data. In addition, all of GeLEA genes were introduced into Escherichia coli to assess the function of GeLEAs under low temperature stress. RESULTS: Based on the phylogenetic analysis with Arabidopsis and Oryza LEA proteins, we identified 8 LEA protein encoding genes in G. elata that could be classified into 6 distinct subgroups. The motif composition of these proteins was an important feature specific to LEA groups. Compared with control, the expressions of 5 GeLEAs in E. coli exhibited enhanced cold resistance and viability, indicating that GeLEAs protein could play a protective role in cells under low temperature stress. CONCLUSION: Our results suggest that LEAs from G. elata play an important role in responses to abiotic stress. [Mh] Termos MeSH primário: Escherichia coli/crescimento & desenvolvimento Gastrodia/metabolismo Proteínas de Plantas/genética Proteínas de Plantas/metabolismo [Mh] Termos MeSH secundário: Clonagem Molecular Temperatura Baixa Escherichia coli/genética Evolução Molecular Gastrodia/genética Regulação da Expressão Gênica de Plantas Família Multigênica Filogenia Estresse Fisiológico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Proteins); 0 (late embryogenesis abundant protein, plant) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180202 [Lr] Data última revisão: 180202 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180107 [St] Status: MEDLINE

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[PMID]: 28489907 [Au] Autor: Luo L; Kim SW; Lee HK; Kim ID; Lee H; Lee JK [Ad] Endereço: Department of Anatomy, Inha University School of Medicine, Inchon, Korea. [Ti] Título: Anti-oxidative effects of 4-hydroxybenzyl alcohol in astrocytes confer protective effects in autocrine and paracrine manners. [So] Source: PLoS One;12(5):e0177322, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: 4-Hydroxybenzyl alcohol (4-HBA) is an important phenolic constituent of Gastrodia elata Blume (GEB), a traditional herbal medicine used in East Asia. Many activities have been reported to underlie the beneficial effects of 4-HBA in the brain, and in particular, its anti-inflammatory, anti-oxidative, and anti-zinc-toxic effects have been implicated in the postischemic brain. Here, the authors investigated the anti-oxidative effect of 4-HBA on astrocytes and sought to identify the underlying molecular mechanisms involved. 4-HBA dose-dependently suppressed H2O2-induced astrocyte cell death. More specifically, pre-incubation of C6 cells (an astrocyte cell line) with 100 µM 4-HBA for 6 hrs increased survival when cells were treated with H2O2 (100 µM, 1 hr) from 54.2±0.7% to 85.9±1.5%. In addition, 4-HBA was found to up-regulate and activate Nrf2, and subsequently, to induce the expressions of several anti-oxidative genes, such as, HO-1, NQO1, and GCLM. Notably, HO-1 was induced by 3.4-fold in 4-HBA-treated C6 cells, and siRNA-mediated HO-1 knockdown demonstrated that Nrf2 activation and HO-1 induction were responsible for the observed cytoprotective effect of 4-HBA. ERK and Akt signaling pathways were activated by 4-HBA in C6 cells, suggesting their involvements in protective effect of 4-HBA. In addition, 4-HBA-conditioned astrocyte culture medium was found to have neuroprotective effects on primary neuronal cultures or fresh C6 cells exposed to oxidative stress, and these effects seemed to be mediated by glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), which both accumulated in 4-HBA-treated astrocyte culture media. Thus, the 4-HBA-mediated activation of Nrf2 and induction of HO-1 in astrocytes were found to act via autocrine and paracrine mechanisms to confer protective effects. Furthermore, given the pleiotropic effects of 4-HBA with respect to its targeting of various brain cell types and functions, it would appear that 4-HBA has therapeutic potential for the prevention and amelioration of various brain diseases. [Mh] Termos MeSH primário: Antioxidantes/farmacologia Astrócitos/efeitos dos fármacos Álcoois Benzílicos/farmacologia Morte Celular/efeitos dos fármacos Fármacos Neuroprotetores/farmacologia Estresse Oxidativo/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Antioxidantes/química Astrócitos/citologia Astrócitos/metabolismo Álcoois Benzílicos/química Células Cultivadas Feminino Gastrodia/química Fator Neurotrófico Derivado de Linhagem de Célula Glial/metabolismo Heme Oxigenase-1/metabolismo Peróxido de Hidrogênio/metabolismo Sistema de Sinalização das MAP Quinases/efeitos dos fármacos Camundongos Camundongos Endogâmicos ICR Fator 2 Relacionado a NF-E2/metabolismo Fármacos Neuroprotetores/química Proteínas Proto-Oncogênicas c-akt/metabolismo Regulação para Cima/efeitos dos fármacos Fator A de Crescimento do Endotélio Vascular/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Benzyl Alcohols); 0 (Glial Cell Line-Derived Neurotrophic Factor); 0 (NF-E2-Related Factor 2); 0 (Neuroprotective Agents); 0 (Vascular Endothelial Growth Factor A); 1A3AH1FP1B (4-hydroxybenzyl alcohol); BBX060AN9V (Hydrogen Peroxide); EC 1.14.14.18 (Heme Oxygenase-1); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170908 [Lr] Data última revisão: 170908 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170511 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0177322

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[PMID]: 28231745 [Au] Autor: Kim NH; Xin MJ; Cha JY; Ji SJ; Kwon SU; Jee HK; Park MR; Park YS; Kim CT; Kim DK; Lee YM [Ad] Endereço: * Department of Oriental Pharmacy, College of Pharmacy and Department of Food Industry Convergence, Republic of Korea. [Ti] Título: Antitumor and Immunomodulatory Effect of Gastrodia elata on Colon Cancer In Vitro and In Vivo. [So] Source: Am J Chin Med;45(2):319-335, 2017. [Is] ISSN: 0192-415X [Cp] País de publicação: Singapore [La] Idioma: eng [Ab] Resumo: Gastrodia elata Blume (GE) is a well-known kind of herb that has been used in traditional medicine for thousands of years. The extrusion of raw materials from it could improve flavor and enhance bioavailability in food and drug development. The purpose of this study is to investigate antitumor and immune boosting effects of extruded GE in human colon carcinoma cells, splenocytes, and mice-bearing CT26 colon carcinoma cell. Treatment with 100[Formula: see text][Formula: see text]g/mL of extruded GE decreased cell viability and induced the expression of Caspase-3 and Bax in HT29 cells ([Formula: see text]). When we performed DAPI staining, apoptotic bodies with condensed chromatin and fragmented nuclei, known as indicative of apoptotic morphology, increased 24[Formula: see text]h after treatment with 100[Formula: see text][Formula: see text]g/mL of extruded GE. Treatments with extruded GE significantly promoted splenocyte proliferation and IL-2 or IFN-[Formula: see text] secretion, compared with that of control cells ([Formula: see text]). The administration of extruded GE of 200 mg/kg/day decreased tumor growth and Ki-67 or [Formula: see text]-catenin expression in mice ([Formula: see text]). Additionally, we investigated the contents of compounds in extruded GE extracts using ultra performance liquid chromatography. The contents of p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde in extruded GE were 2.97[Formula: see text]mg/g and 0.04[Formula: see text]mg/g, respectively. It was supposed that antitumor and immunomodulatory effects of extruded GE might exert by the p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde of many compositions analyzed from extruded GE. These results suggest that extruded GE have the potential to be developed into a natural pharmaceutical and functional food as a cancer chemopreventive agent. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Neoplasias do Colo/tratamento farmacológico Neoplasias do Colo/patologia Gastrodia Fatores Imunológicos Extratos Vegetais/farmacologia Baço/imunologia [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/uso terapêutico Proliferação Celular/efeitos dos fármacos Neoplasias do Colo/genética Expressão Gênica/efeitos dos fármacos Células HT29 Seres Humanos Antígeno Ki-67/genética Antígeno Ki-67/metabolismo Masculino Camundongos Camundongos Endogâmicos BALB C Extratos Vegetais/uso terapêutico Baço/citologia beta Catenina/genética beta Catenina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Immunologic Factors); 0 (Ki-67 Antigen); 0 (Plant Extracts); 0 (beta Catenin) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170508 [Lr] Data última revisão: 170508 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170225 [St] Status: MEDLINE [do] DOI: 10.1142/S0192415X17500203

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[PMID]: 27936399 [Au] Autor: Li Y; Wang LM; Xu JZ; Tian K; Gu CX; Li ZF [Ad] Endereço: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China. [Ti] Título: Gastrodia elata attenuates inflammatory response by inhibiting the NF-κB pathway in rheumatoid arthritis fibroblast-like synoviocytes. [So] Source: Biomed Pharmacother;85:177-181, 2017 Jan. [Is] ISSN: 1950-6007 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS. GE also inhibited TNF-α-induced MMP-3 and MMP-13 expression in RA-FLS. Furthermore, pretreatment with GE significantly attenuated TNF-α-induced the expression of p-p65 and IκBα degradation in RA-FLS. In conclusion, this study demonstrated for the first time that GE attenuated inflammatory response by inhibiting the NF-κB pathway signaling in RA-FLS. Thus, GE might have a therapeutic potential towards the treatment of RA. [Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia Antirreumáticos/farmacologia Artrite Reumatoide/tratamento farmacológico Fibroblastos/efeitos dos fármacos Gastrodia Mediadores da Inflamação/metabolismo NF-kappa B/metabolismo Extratos Vegetais/farmacologia Membrana Sinovial/efeitos dos fármacos [Mh] Termos MeSH secundário: Anti-Inflamatórios/isolamento & purificação Antirreumáticos/isolamento & purificação Artrite Reumatoide/genética Artrite Reumatoide/metabolismo Artrite Reumatoide/patologia Relação Dose-Resposta a Droga Fibroblastos/metabolismo Fibroblastos/patologia Gastrodia/química Seres Humanos Interleucina-6/metabolismo Interleucina-8/metabolismo Metaloproteinase 13 da Matriz/genética Metaloproteinase 13 da Matriz/metabolismo Metaloproteinase 3 da Matriz/genética Metaloproteinase 3 da Matriz/metabolismo Inibidor de NF-kappaB alfa/metabolismo Fosforilação Fitoterapia Extratos Vegetais/isolamento & purificação Plantas Medicinais Proteólise Transdução de Sinais/efeitos dos fármacos Membrana Sinovial/metabolismo Membrana Sinovial/patologia Fatores de Tempo Fator de Transcrição RelA/metabolismo Fator de Necrose Tumoral alfa/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Antirheumatic Agents); 0 (IL6 protein, human); 0 (IL8 protein, human); 0 (Inflammation Mediators); 0 (Interleukin-6); 0 (Interleukin-8); 0 (NF-kappa B); 0 (Plant Extracts); 0 (RELA protein, human); 0 (Transcription Factor RelA); 0 (Tumor Necrosis Factor-alpha); 139874-52-5 (NF-KappaB Inhibitor alpha); EC 3.4.24.- (MMP13 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 13); EC 3.4.24.17 (MMP3 protein, human); EC 3.4.24.17 (Matrix Metalloproteinase 3) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170213 [Lr] Data última revisão: 170213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161210 [St] Status: MEDLINE

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[PMID]: 27925254 [Au] Autor: Jiang Z; Zheng X; Gong X; Zhao C; Zhou X; Zhao Y; Yan Y [Ad] Endereço: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China. [Ti] Título: Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC-ESI-MS/MS. [So] Source: Biomed Chromatogr;31(7), 2017 Jul. [Is] ISSN: 1099-0801 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: New research has indicated that Gastrodiae Rhizome (GR) has potential anti-diabetic and anti-asthmatic effects in mouse models. On the basis of our previous study of the relative bioavailability of gastrodin (GAS) and parishin (PA) from extract and powder of GR, we performed further research on the tissue distribution and excretion of the two analytes. A reliable bioanalytical method for the quantification of GAS and PA in rat tissues and excretion is required. Chromatographic separation was carried out on a gradient mobile phase of acetonitrile-water with 0.1% formic acid. Calibration curves (1/x weighted) offered satisfactory linearity (r > 0.9835) within 100-3000 ng mL for GAS and (r > 0.9862) within 10-1000 ng mL for PA. The relative standard deviations of the intra-day and inter-day precision were all <14.98%, whilst the relative errors of the intra-day and inter-day accuracy were all within ±14.71%. The matrix effect and recovery values were satisfactory in all of the biological matrices examination. The data of relative differences in tissue distribution and excretion of GAS and PA from powder and extract of GR indicated that higher bioavailabilities for GAS and PA were obtained when a dosage of 4 g kg GR powder was used. [Mh] Termos MeSH primário: Álcoois Benzílicos/farmacocinética Cromatografia Líquida de Alta Pressão/métodos Gastrodia/química Glucosídeos/farmacocinética Extratos Vegetais/farmacocinética Pós Espectrometria de Massas por Ionização por Electrospray/métodos [Mh] Termos MeSH secundário: Animais Disponibilidade Biológica Limite de Detecção Masculino Ratos Ratos Sprague-Dawley Reprodutibilidade dos Testes Distribuição Tecidual [Pt] Tipo de publicação: JOURNAL ARTICLE; VALIDATION STUDIES [Nm] Nome de substância: 0 (Benzyl Alcohols); 0 (Glucosides); 0 (Plant Extracts); 0 (Powders); 5YS9U2W3RQ (gastrodin) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170704 [Lr] Data última revisão: 170704 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161208 [St] Status: MEDLINE [do] DOI: 10.1002/bmc.3909

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[PMID]: 28852737 [Au] Autor: He F; Duan X; Dai R; Wang W; Yang C; Lin Q [Ad] Endereço: The Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. [Ti] Título: PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION. [So] Source: Afr J Tradit Complement Altern Med;13(4):199-209, 2016. [Is] ISSN: 2505-0044 [Cp] País de publicação: Nigeria [La] Idioma: eng [Ab] Resumo: BACKGROUND: Damage of the blood brain barrier (BBB) during the process of cerebral ischemic injury is a key factor which influences the therapeutic efficacy to the cerebral ischemic injury. The present study was designed to verify the mechanisms underlying the protective effects of the ethyl acetate (EtOAc) extraction from Gastrodia elata Blume (GEB) on the BBB by developing a model of cerebral ischemia-reperfusion in rats. MATERIAL AND METHODS: MCAO/R model in rats was developed through a thread embolism method. The neurological scales, the moisture and the evans blue (EB) contents of brains were detected. Meanwhile, the release of nitric oxide (NO) and activities of NO synthase (NOS) in brain tissues were measured. Western blotting analyses were also performed to assess the protein expressions of AQP-4, Occludin and Claudin-5 in brain tissue. RESULTS: After rats were pretreated with different concentrations of EtOAc extractions from GEB, the neurologic scores, the EB contents in the brain tissues and the moisture of the brains were significantly decreased. Meanwhile, the release of NO, the activities of nNOS and iNOS were notably inhibited. Furthemore, the protein expression of AQP-4 was markedly decreased, but the protein expressions of -5 and Occludin were significantly increased. CONCLUSION: the EtOAc extracts of GEB may decrease the permeability of BBB when focal cerebral ischemia occurs. The inhibition of the NOS pathways, the attenuation of the protein expression of AQP-4 and the enhancement of the expressions of the tight junction proteins may contribute to the protective effects of the EtOAc extracts from GEB on BBB. [Mh] Termos MeSH primário: Barreira Hematoencefálica/efeitos dos fármacos Medicamentos de Ervas Chinesas/administração & dosagem Gastrodia/química Substâncias Protetoras/administração & dosagem Traumatismo por Reperfusão/prevenção & controle [Mh] Termos MeSH secundário: Acetatos/química Animais Aquaporina 4/genética Aquaporina 4/metabolismo Barreira Hematoencefálica/metabolismo Isquemia Encefálica/cirurgia Seres Humanos Masculino Óxido Nítrico/metabolismo Óxido Nítrico Sintase/genética Óxido Nítrico Sintase/metabolismo Ocludina/genética Ocludina/metabolismo Ratos Ratos Sprague-Dawley Traumatismo por Reperfusão/genética Traumatismo por Reperfusão/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Acetates); 0 (Aqp4 protein, rat); 0 (Aquaporin 4); 0 (Drugs, Chinese Herbal); 0 (Occludin); 0 (Protective Agents); 31C4KY9ESH (Nitric Oxide); 76845O8NMZ (ethyl acetate); EC 1.14.13.39 (Nitric Oxide Synthase) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170908 [Lr] Data última revisão: 170908 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170831 [St] Status: MEDLINE [do] DOI: 10.21010/ajtcam.v13i4.26

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[PMID]: 28033308 [Au] Autor: Li HB; Wu F; Miao HC; Xiong KR [Ad] Endereço: Department of Anatomy, Wannan Medical College, Wuhu, Anhui, China (mainland). [Ti] Título: Effects of Polysaccharide of Gastrodia Elata Blume and Electro-Acupuncture on Expressions of Brain-Derived Neurotrophic Factor and Stem Cell Factor Protein in Caudate Putamen of Focal Cerebral Ischemia Rats. [So] Source: Med Sci Monit Basic Res;22:175-180, 2016 Dec 23. [Is] ISSN: 2325-4416 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND The aim of this study was to explore the neural protective effect of polysaccharide of Gastrodia elata Blume (PGB) and electro-acupuncture (EA) on focal cerebral ischemia rats. MATERIAL AND METHODS A total of 40 Sprague-Dawley rats were randomly divided into 5 groups (normal group, model group, PGB group, EA group and PGB+EA group). The model was prepared by middle cerebral artery occlusion (MCAO). Two week after modeling, rats were given PGB, EA, or a combination of the 2 in continuous treatment for 2 successive weeks. 14 days after modeling, expressions of BDNF and SCF protein in the caudate putamen (CPu) were detected by immunohistochemistry. RESULTS Positive expression of BDNF and SCF protein was found in the right caudate putamen of each group of rats. Expressions of BDNF and SCF in the CPu of the model group were higher than normal group (P<0.05). Compared with the model group, the expressions of BDNF and SCF in the CPu of the PGB group, the EA group, and the PGB plus EA group increased significantly (P<0.05). The expressions of BDNF and SCF obviously increased in the PGB plus EA group compared to those of the EA group and the PGB group (P<0.05). CONCLUSIONS PGB and EA up-regulated the expressions of BDNF and SCF protein in the CPu of focal cerebral ischemia rats, and the combination of PGB+EA has a synergistic effect on the recovery from cerebral ischemia. [Mh] Termos MeSH primário: Terapia por Acupuntura Isquemia Encefálica/tratamento farmacológico Fator Neurotrófico Derivado do Encéfalo/metabolismo Gastrodia/química Polissacarídeos/uso terapêutico Putamen/patologia Fator de Células-Tronco/metabolismo [Mh] Termos MeSH secundário: Animais Isquemia Encefálica/patologia Contagem de Células Masculino Polissacarídeos/farmacologia Putamen/efeitos dos fármacos Ratos Sprague-Dawley [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Brain-Derived Neurotrophic Factor); 0 (Polysaccharides); 0 (Stem Cell Factor) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170424 [Lr] Data última revisão: 170424 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161230 [St] Status: MEDLINE [do] DOI: 10.12659/MSMBR.901524

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[PMID]: 27845865 [Au] Autor: Song E; Chung H; Shim E; Jeong JK; Han BK; Choi HJ; Hwang J [Ad] Endereço: 1 Department of Food and Nutrition, College of Natural Sciences, Myongji University , Yongin, Korea. [Ti] Título: Gastrodia elata Blume Extract Modulates Antioxidant Activity and Ultraviolet A-Irradiated Skin Aging in Human Dermal Fibroblast Cells. [So] Source: J Med Food;19(11):1057-1064, 2016 Nov. [Is] ISSN: 1557-7600 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Gastrodia elata Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (e.g., paralysis and stroke) and skin problems (e.g., atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate the antioxidant ability of GEB and its antiaging effect on human dermal fibroblast cells (HDF). The total phenolic and flavonoid contents of GEB were 21.8 and 0.43 mg/g dry weight (DW), respectively. The ergothioneine content of GEB was 0.41 mg/mL DW. The DPPH and ABTS radical scavenging activities of GEB at 5 and 10 mg/mL approximately ranged between 31% and 44%. The superoxide dismutase activity of GEB at 10 and 25 mg/mL was 57% and 76%, respectively. GEB increased procollagen type 1 (PC1) production and inhibited matrix metalloproteinase-1 (MMP-1) production and elastase-1 activity in UVA-irradiated HDF. PC1 messenger RNA (mRNA) levels decreased upon UVA irradiation, but recovered in response to high doses of GEB in HDF. On the contrary, GEB significantly decreased MMP-1 and elastase-1 mRNA levels, which were markedly induced in UVA-irradiated HDF. Collectively, these results suggest that GEB has sufficient antioxidant ability to prevent the signs of skin aging in UVA-irradiated human skin cells, suggesting its potential as a natural antiaging product. [Mh] Termos MeSH primário: Antioxidantes/farmacologia Gastrodia/química Extratos Vegetais/farmacologia Envelhecimento da Pele/efeitos dos fármacos Pele/efeitos dos fármacos Pele/efeitos da radiação [Mh] Termos MeSH secundário: Linhagem Celular Sobrevivência Celular/efeitos dos fármacos Colágeno Tipo I/metabolismo Ativação Enzimática/efeitos dos fármacos Fibroblastos/efeitos dos fármacos Fibroblastos/enzimologia Fibroblastos/efeitos da radiação Seres Humanos Metaloproteinase 1 da Matriz/metabolismo Elastase Pancreática/metabolismo RNA Mensageiro/metabolismo Pele/citologia Pele/enzimologia Envelhecimento da Pele/patologia Superóxido Dismutase/metabolismo Raios Ultravioleta/efeitos adversos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Collagen Type I); 0 (Plant Extracts); 0 (RNA, Messenger); EC 1.15.1.1 (Superoxide Dismutase); EC 3.4.21.36 (Pancreatic Elastase); EC 3.4.24.7 (MMP1 protein, human); EC 3.4.24.7 (Matrix Metalloproteinase 1) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170526 [Lr] Data última revisão: 170526 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161116 [St] Status: MEDLINE

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[PMID]: 27765372 [Au] Autor: Matias M; Silvestre S; Falcão A; Alves G [Ad] Endereço: CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Rua Marquês d'Ávila e Bolama, 6201-001 Covilhã, Portugal; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal. [Ti] Título: Gastrodia elata and epilepsy: Rationale and therapeutic potential. [So] Source: Phytomedicine;23(12):1511-1526, 2016 Nov 15. [Is] ISSN: 1618-095X [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: BACKGROUND: Gastrodia elata Blume (G. elata) is a traditional Chinese herb used for centuries in folk medicine. Due to the claimed anticonvulsant properties of G. elata, it is expected that this herb continues to be a target of research, aiming to deepen the available knowledge on its biological activity and safety. PURPOSE: The current review aims to discuss the most recent advances on the elucidation of the phytochemical composition and anticonvulsant potential of G. elata. METHODS: Available literature was reviewed from PubMed, ISI Web of Knowledge and Science Direct, using combinations of the following keywords: Gastrodia elata, tianma, epilepsy, anticonvulsant and pharmacokinetics. Abstracts and full texts were evaluated for their clarity and scientific merit. RESULTS: G. elata rhizome, as well as specific phenolic compounds isolated from this herb, have demonstrated anticonvulsant potential in a variety of in vitro and in vivo models. The pharmacological mechanisms potentially involved in the anticonvulsant activity have been extensively studied, being similar to the known mechanisms claimed for the available antiepileptic drugs. In addition, the pharmacokinetics of the main bioactive component of G. elata (gastrodin) has also been studied. CONCLUSION: Due to its recognised therapeutic properties, G. elata has gained an increasing interest within the scientific community and, therefore, new medicinal preparations containing G. elata rhizome itself or its bioactive components are expected to be developed in the coming years. Moreover, specific phytochemical constituents isolated from G. elata may also be considered to integrate programs of discovery and development of new anticonvulsant drug candidates. [Mh] Termos MeSH primário: Anticonvulsivantes/farmacologia Álcoois Benzílicos/farmacologia Medicamentos de Ervas Chinesas/farmacologia Epilepsia Gastrodia/química Glucosídeos/farmacologia Fenóis/farmacologia Fitoterapia [Mh] Termos MeSH secundário: Anticonvulsivantes/uso terapêutico Álcoois Benzílicos/farmacocinética Álcoois Benzílicos/uso terapêutico Medicamentos de Ervas Chinesas/química Medicamentos de Ervas Chinesas/uso terapêutico Epilepsia/tratamento farmacológico Glucosídeos/farmacocinética Glucosídeos/uso terapêutico Seres Humanos Fenóis/uso terapêutico Rizoma/química [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Anticonvulsants); 0 (Benzyl Alcohols); 0 (Drugs, Chinese Herbal); 0 (Glucosides); 0 (Phenols); 5YS9U2W3RQ (gastrodin) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161022 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 27732148 [Au] Autor: Huan T; Xian JW; Leung WN; Li L; Chan CW [Ad] Endereço: 1 Department of Chemistry, University of Alberta , Edmonton, Alberta, Canada . [Ti] Título: Cerebrospinal Fluid Metabolomics After Natural Product Treatment in an Experimental Model of Cerebral Ischemia. [So] Source: OMICS;20(11):670-680, 2016 Nov. [Is] ISSN: 1557-8100 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Cerebrospinal fluid (CSF) is an important biofluid for diagnosis of and research on neurological diseases. However, in-depth metabolomic profiling of CSF remains an analytical challenge due to the small volume of samples, particularly in small animal models. In this work, we report the application of a high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) workflow for CSF metabolomics in Gastrodia elata and Uncaria rhynchophylla water extract (GUW)-treated experimental cerebral ischemia model of rat. The GUW is a commonly used Traditional Chinese Medicine (TCM) for hypertension and brain disease. This study investigated the amine- and phenol-containing biomarkers in the CSF metabolome. After GUW treatment for 7 days, the neurological deficit score was significantly improved with infarct volume reduction, while the integrity of brain histological structure was preserved. Over 1957 metabolites were quantified in CSF by dansylation LC-MS. The analysis of this comprehensive list of metabolites suggests that metabolites associated with oxidative stress, inflammatory response, and excitotoxicity change during GUW-induced alleviation of ischemic injury. This work is significant in that (1) it shows CIL LC-MS can be used for in-depth profiling of the CSF metabolome in experimental ischemic stroke and (2) identifies several potential molecular targets (that might mediate the central nervous system) and associate with pharmacodynamic effects of some frequently used TCMs. [Mh] Termos MeSH primário: Produtos Biológicos/uso terapêutico Isquemia Encefálica/líquido cefalorraquidiano Isquemia Encefálica/terapia Proteínas do Líquido Cefalorraquidiano/metabolismo Metabolômica/métodos Acidente Vascular Cerebral/líquido cefalorraquidiano Acidente Vascular Cerebral/terapia [Mh] Termos MeSH secundário: Aminas/análise Animais Biomarcadores/análise Biomarcadores/metabolismo Isquemia Encefálica/patologia Proteínas do Líquido Cefalorraquidiano/análise Cromatografia Líquida/métodos Modelos Animais de Doenças Medicamentos de Ervas Chinesas/uso terapêutico Gastrodia Masculino Espectrometria de Massas/métodos Medicina Tradicional Chinesa Fenóis/análise Ratos Ratos Sprague-Dawley Acidente Vascular Cerebral/patologia Uncaria [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amines); 0 (Biological Products); 0 (Biomarkers); 0 (Cerebrospinal Fluid Proteins); 0 (Drugs, Chinese Herbal); 0 (Phenols) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170929 [Lr] Data última revisão: 170929 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161013 [St] Status: MEDLINE

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