Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.875.088 [Categoria DeCS]
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  1 / 17 MEDLINE  
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[PMID]:23950788
[Au] Autor:Kim JS; Kim JH
[Ad] Endereço:Department of Life Science, Gacheon University, Seongnam, Gyeonggi-do, South Korea. kimjh2009@gachon.ac.kr
[Ti] Título:Comparative genome analysis and phylogenetic relationship of order Liliales insight from the complete plastid genome sequences of two Lilies (Lilium longiflorum and Alstroemeria aurea).
[So] Source:PLoS One;8(6):e68180, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Monocots are one of the most diverse, successful and economically important clades of angiosperms. We attempt to analyse the complete plastid genome sequences of two lilies and their lengths were 152,793bp in Lilium longiflorum (Liliaceae) and 155,510bp in Alstroemeria aurea (Alstroemeriaceae). Phylogenetic analyses were performed for 28 taxa including major lineages of monocots using the sequences of 79 plastid genes for clarifying the phylogenetic relationship of the order Liliales. The sister relationship of Liliales and Asparagales-commelinids was improved with high resolution. Comparative analyses of inter-familial and inter-specific sequence variation were also carried out among three families of Liliaceae, Smilacaceae, and Alstroemeriaceae, and between two Lilium species of L. longflorum and L. superbum. Gene content and order were conserved in the order Liliales except infA loss in Smilax and Alstroemeria. IR boundaries were similar in IRa, however, IRb showed different extension patterns as JLB of Smilax and JSB in Alstroemeria. Ka/Ks ratio was high in matK among the pair-wise comparison of three families and the most variable genes were psaJ, ycf1, rpl32, rpl22, matK, and ccsA among the three families and rps15, rpoA, matK, and ndhF between Lilium.
[Mh] Termos MeSH primário: Alstroemeria/genética
Genomas de Plastídeos
Lilium/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Filogenia
Plastídeos/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1404
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130817
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0068180


  2 / 17 MEDLINE  
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[PMID]:22956470
[Au] Autor:Chacón J; Sousa A; Baeza CM; Renner SS
[Ad] Endereço:Systematic Botany and Mycology, University of Munich, 80638 Munich, Germany. juliana.chacon@bio.lmu.de
[Ti] Título:Ribosomal DNA distribution and a genus-wide phylogeny reveal patterns of chromosomal evolution in Alstroemeria (Alstroemeriaceae).
[So] Source:Am J Bot;99(9):1501-12, 2012 Sep.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Understanding the flexibility of monocot genomes requires a phylogenetic framework, which so far is available for few of the ca. 2800 genera. Here we use a molecular tree for the South American genus Alstroemeria to place karyological information, including fluorescent in situ hybridization (FISH) signals, in an explicit evolutionary context. METHODS: From a phylogeny based on plastid, nuclear, and mitochondrial sequences for most species of Alstroemeria, we selected early-branching (Chilean) and derived (Brazilian) species for which we obtained 18S-25S and 5S rDNA FISH signals; we also analyzed chromosome numbers, 1C-values, and telomere FISH signals (in two species). KEY RESULTS: Chromosome counts for Alstroemeria cf. rupestris and A. pulchella confirm 2n = 16 as typical of the genus, which now has chromosomes counted for 29 of its 78 species. The rDNA sites are polymorphic both among and within species, and interstitial telomeric sites in Alstroemeria cf. rupestris suggest chromosome fusion. CONCLUSIONS: In spite of a constant chromosome number, closely related species of Alstroemeria differ drastically in their rDNA, indicating rapid increase, decrease, or translocations of these genes. Previously proposed Brazilian and Chilean karyotype groups are not natural, and the n = 8 chromosomes in Alstroemeria compared to n = 9 in its sister genus Bomarea may result from a Robertsonian fusion.
[Mh] Termos MeSH primário: Alstroemeria/genética
Cromossomos de Plantas/genética
DNA de Plantas/genética
DNA Ribossômico/genética
Evolução Molecular
Filogenia
[Mh] Termos MeSH secundário: Sequência de Bases
Hibridização in Situ Fluorescente
Cariotipagem
Funções Verossimilhança
Mitose/genética
Especificidade da Espécie
Telômero/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:120907
[Lr] Data última revisão:
120907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120908
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1200104


  3 / 17 MEDLINE  
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[PMID]:22325893
[Au] Autor:Hirai M; Yamagishi M; Kanno A
[Ad] Endereço:Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo 060-8589, Japan. a.ligtu@gmail.com
[Ti] Título:Reduced transcription of a LEAFY-like gene in Alstroemeria sp. cultivar Green Coral that cannot develop floral meristems.
[So] Source:Plant Sci;185-186:298-308, 2012 Apr.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Alstroemeria sp. cv. Green Coral has numerous bracts instead of flowers, and its cyme structures are repeated eternally. Observations of the development and morphology of inflorescence in cv. Green Coral revealed that transition from inflorescence to floral meristem was restricted. We isolated and characterized floral meristem identity genes LEAFY-like (AlsLFY) and SQUAMOSA-like (AlsSQa and AlsSQb) genes from Alstroemeria ligtu. In situ hybridization results indicated that AlsSQa and AlsSQb were expressed in the dome-shaped floral meristems and all floral organ primordia in A. ligtu. Transcripts of AlsLFY accumulated early in the dome-shaped floral meristems; the signals were restricted later to the outer region of the floral meristem. These results indicate that AlsLFY, AlsSQa, and AlsSQb function as floral meristem identity genes. Expression profiles of AlsLFY, AlsSQa, AlsSQb, and other MADS-box genes were compared between A. ligtu and cv. Green Coral. AlsLFY, AlsDEFa, and AlsAGL6 transcripts were not detected at the shoot apices of cv. Green Coral but were detected in A. ligtu. The early induction and accumulation of AlsLFY transcripts in the inflorescence meristem of A. ligtu prior to development of the floral meristem suggest that downregulation of AlsLFY is likely to restrict the inflorescence-to-floral meristem transition in cv. Green Coral.
[Mh] Termos MeSH primário: Alstroemeria/genética
Regulação da Expressão Gênica no Desenvolvimento/genética
Inflorescência/genética
Meristema/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Alstroemeria/citologia
Alstroemeria/crescimento & desenvolvimento
Sequência de Bases
Regulação para Baixo/genética
Regulação da Expressão Gênica de Plantas/genética
Hibridização In Situ
Inflorescência/crescimento & desenvolvimento
Proteínas de Domínio MADS/genética
Proteínas de Domínio MADS/metabolismo
Meristema/crescimento & desenvolvimento
Dados de Sequência Molecular
Mutação
Fenótipo
Filogenia
Folhas de Planta/citologia
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Proteínas de Plantas/metabolismo
Brotos de Planta/citologia
Brotos de Planta/genética
Brotos de Planta/crescimento & desenvolvimento
Análise de Sequência
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MADS Domain Proteins); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:120213
[Lr] Data última revisão:
120213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120214
[St] Status:MEDLINE
[do] DOI:10.1016/j.plantsci.2011.12.017


  4 / 17 MEDLINE  
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[PMID]:22268153
[Au] Autor:Aros D; Gonzalez V; Allemann RK; Müller CT; Rosati C; Rogers HJ
[Ad] Endereço:School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK.
[Ti] Título:Volatile emissions of scented Alstroemeria genotypes are dominated by terpenes, and a myrcene synthase gene is highly expressed in scented Alstroemeria flowers.
[So] Source:J Exp Bot;63(7):2739-52, 2012 Apr.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Native to South America, Alstroemeria flowers are known for their colourful tepals, and Alstroemeria hybrids are an important cut flower. However, in common with many commercial cut flowers, virtually all the commercial Alstroemeria hybrids are not scented. The cultivar 'Sweet Laura' is one of very few scented commercial Alstroemeria hybrids. Characterization of the volatile emission profile of these cut flowers revealed three major terpene compounds: (E)-caryophyllene, humulene (also known as α-caryophyllene), an ocimene-like compound, and several minor peaks, one of which was identified as myrcene. The profile is completely different from that of the parental scented species A. caryophyllaea. Volatile emission peaked at anthesis in both scented genotypes, coincident in cv. 'Sweet Laura' with the maximal expression of a putative terpene synthase gene AlstroTPS. This gene was preferentially expressed in floral tissues of both cv. 'Sweet Laura' and A. caryophyllaea. Characterization of the AlstroTPS gene structure from cv. 'Sweet Laura' placed it as a member of the class III terpene synthases, and the predicted 567 amino acid sequence placed it into the subfamily TPS-b. The conserved sequences R(28)(R)X(8)W and D(321)DXXD are the putative Mg(2+)-binding sites, and in vitro assay of AlstroTPS expressed in Escherichia coli revealed that the encoded enzyme possesses myrcene synthase activity, consistent with a role for AlstroTPS in scent production in Alstroemeria cv. 'Sweet Laura' flowers.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo
Alstroemeria/enzimologia
Flores/enzimologia
Liases Intramoleculares/metabolismo
Proteínas de Plantas/genética
Terpenos/metabolismo
[Mh] Termos MeSH secundário: Alcenos/química
Alcenos/metabolismo
Alquil e Aril Transferases/genética
Alstroemeria/classificação
Alstroemeria/genética
Alstroemeria/metabolismo
Sequência de Aminoácidos
Flores/genética
Flores/metabolismo
Regulação da Expressão Gênica de Plantas
Genótipo
Liases Intramoleculares/genética
Dados de Sequência Molecular
Monoterpenos/química
Monoterpenos/metabolismo
Filogenia
Proteínas de Plantas/metabolismo
Alinhamento de Sequência
Terpenos/química
Volatilização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkenes); 0 (Monoterpenes); 0 (Plant Proteins); 0 (Terpenes); 3M39CZS25B (myrcene); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase); EC 5.5.- (Intramolecular Lyases)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120124
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/err456


  5 / 17 MEDLINE  
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[PMID]:21169212
[Au] Autor:Koenig R; Lesemann DE; Pfeilstetter E; Winter S; Pleij CW
[Ad] Endereço:Julius Kühn-Institute, Federal Research Center for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, and DSMZ Plant Virus Department, Messeweg 11, D38104 Braunschweig, Germany. renate.koenig@jki.bund.de
[Ti] Título:Deletions and recombinations with the RNA1 3' ends of different tobraviruses have created a multitude of tobacco rattle virus TCM-related RNA2 species in Alstroemeria and tulip.
[So] Source:J Gen Virol;92(Pt 4):988-96, 2011 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In vegetatively propagated Alstroemeria plants that showed pronounced stunting and necrotic leaf spots, a tobravirus infection was diagnosed in which one tobacco rattle virus (TRV, strain AL) RNA1 species was associated with seven different RNA2 species. The latter differed considerably in size and in the types of their 3' RNA1-related sequences. The 5' RNA2-specific part of all these RNA2 molecules showed almost 100% sequence identity with that of RNA2 of the TRV isolate TCM from tulip, but in some of these RNA2 molecules it was shorter than in the TCM isolate, whereas in others it was longer. One of the TRV AL RNA2 molecules, i.e. TC3'PE-a, contained the full set of three full-length RNA2-specific ORFs (ORF2a, -2b and -2c), whereas the previously analysed TCM sequence contained only ORF2a and -2b. In four of these TRV AL RNA2 molecules, i.e. those that had a relatively short RNA2-specific part, the 3' end was identical to that of the cognate TRV AL RNA1, but in the other three, which had a long RNA2-specific part, it was closely related to that of pea early browning virus (PEBV) RNA1, which was not detected in the infected plants. A comparison with previously described TRV/PEBV RNA2 recombinants suggested that the various TRV AL RNA2 molecules may represent various steps and side steps in an evolutionary process, which is apt to open the wide host range of TRV also to PEBV-derived RNA2 species.
[Mh] Termos MeSH primário: Alstroemeria/virologia
Vírus de Plantas/genética
Vírus de RNA/genética
Recombinação Genética
Deleção de Sequência
Tulipa/virologia
[Mh] Termos MeSH secundário: Evolução Molecular
Dados de Sequência Molecular
Fases de Leitura Aberta
Doenças das Plantas/virologia
Vírus de Plantas/classificação
Vírus de RNA/classificação
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1105
[Cu] Atualização por classe:110317
[Lr] Data última revisão:
110317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101221
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.028803-0


  6 / 17 MEDLINE  
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[PMID]:20457576
[Au] Autor:Wagstaff C; Bramke I; Breeze E; Thornber S; Harrison E; Thomas B; Buchanan-Wollaston V; Stead T; Rogers H
[Ad] Endereço:Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3TL, UK.
[Ti] Título:A specific group of genes respond to cold dehydration stress in cut Alstroemeria flowers whereas ambient dehydration stress accelerates developmental senescence expression patterns.
[So] Source:J Exp Bot;61(11):2905-21, 2010 Jun.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Petal development and senescence entails a normally irreversible process. It starts with petal expansion and pigment production, and ends with nutrient remobilization and ultimately cell death. In many species this is accompanied by petal abscission. Post-harvest stress is an important factor in limiting petal longevity in cut flowers and accelerates some of the processes of senescence such as petal wilting and abscission. However, some of the effects of moderate stress in young flowers are reversible with appropriate treatments. Transcriptomic studies have shown that distinct gene sets are expressed during petal development and senescence. Despite this, the overlap in gene expression between developmental and stress-induced senescence in petals has not been fully investigated in any species. Here a custom-made cDNA microarray from Alstroemeria petals was used to investigate the overlap in gene expression between developmental changes (bud to first sign of senescence) and typical post-harvest stress treatments. Young flowers were stressed by cold or ambient temperatures without water followed by a recovery and rehydration period. Stressed flowers were still at the bud stage after stress treatments. Microarray analysis showed that ambient dehydration stress accelerates many of the changes in gene expression patterns that would normally occur during developmental senescence. However, a higher proportion of gene expression changes in response to cold stress were specific to this stimulus and not senescence related. The expression of 21 transcription factors was characterized, showing that overlapping sets of regulatory genes are activated during developmental senescence and by different stresses.
[Mh] Termos MeSH primário: Alstroemeria/crescimento & desenvolvimento
Alstroemeria/fisiologia
Regulação da Expressão Gênica no Desenvolvimento
Água/metabolismo
[Mh] Termos MeSH secundário: Alstroemeria/genética
Temperatura Baixa
Flores
Regulação da Expressão Gênica de Plantas
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1009
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100512
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erq113


  7 / 17 MEDLINE  
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[PMID]:20151164
[Au] Autor:Hassani-Mehraban A; Botermans M; Verhoeven JT; Meekes E; Saaijer J; Peters D; Goldbach R; Kormelink R
[Ad] Endereço:Laboratory of Virology, Department of Plant Sciences, Wageningen University, Wageningen, The Netherlands.
[Ti] Título:A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia.
[So] Source:Arch Virol;155(3):423-8, 2010 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.
[Mh] Termos MeSH primário: Alstroemeria/virologia
Doenças das Plantas/virologia
Tospovirus/classificação
Tospovirus/genética
[Mh] Termos MeSH secundário: Clonagem Molecular
Análise por Conglomerados
Colômbia
Dados de Sequência Molecular
Filogenia
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Sorotipagem
Tospovirus/imunologia
Tospovirus/isolamento & purificação
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1003
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100213
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-010-0590-7


  8 / 17 MEDLINE  
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[PMID]:19796365
[Au] Autor:Igawa T; Hoshino Y; Yanagawa Y
[Ad] Endereço:Plant Science Education Unit, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara, 630-0101 Japan.
[Ti] Título:Isolation and characterization of the plant glsA promoter from Alstroemeria.
[So] Source:Plant Biol (Stuttg);11(6):878-85, 2009 Nov.
[Is] ISSN:1435-8603
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The differentiation of a vegetative cell and a generative cell is a critical event during pollen development. The Lilium GlsA is known to localize in pollen and is considered to be involved in development of the generative cell. Here, we cloned a glsA ortholog from Alstroemeria, a commercially important cut flower. The expression of AaglsA (Alstroemeria aurea glsA) transcripts increased gradually after pollen mitosis I (PMI) and reached a significant level when the generative cell started to elongate. Analysis of the promoter of AaglsA suggests that AaglsA expression is controlled by several cis-regulatory elements during pollen development. This is the first investigation of reproductive factors regulating male gametogenesis in Alstroemeria.
[Mh] Termos MeSH primário: Alstroemeria/genética
Proteínas de Plantas/genética
Regiões Promotoras Genéticas
[Mh] Termos MeSH secundário: Alstroemeria/crescimento & desenvolvimento
Alstroemeria/metabolismo
Sequência de Bases
Clonagem Molecular
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Mitose
Dados de Sequência Molecular
Proteínas de Plantas/metabolismo
Pólen/metabolismo
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:0912
[Cu] Atualização por classe:091002
[Lr] Data última revisão:
091002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091003
[St] Status:MEDLINE
[do] DOI:10.1111/j.1438-8677.2008.00177.x


  9 / 17 MEDLINE  
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[PMID]:19151916
[Au] Autor:Hirano T; Hoshino Y
[Ad] Endereço:Division of Innovative Research, Creative Research Initiative Sousei, Hokkaido University, Sapporo 001-0021, Japan. t-hirano@cris.hokudai.ac.jp
[Ti] Título:Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea.
[So] Source:J Plant Res;122(2):225-34, 2009 Mar.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry at different developmental stages of pollen tubes cultured in vitro revealed changes in the nuclear phase at 9 and 18 h after culture. Sperm cell formation occurred at 6-9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cells showed a tendency to increase. This association was suggested as the male germ unit (MGU). When sperm cells, vegetative nuclei, and partial MGUs were collected separately from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial MGUs was counted as 2C. Therefore, the second change in the nuclear phase, which results in an increase in 2C nuclei, is possibly related to the formation of MGUs.
[Mh] Termos MeSH primário: Alstroemeria/genética
Núcleo Celular/metabolismo
Citometria de Fluxo/métodos
Tubo Polínico/crescimento & desenvolvimento
Pólen/citologia
Pólen/metabolismo
[Mh] Termos MeSH secundário: Diferenciação Celular
DNA de Plantas/química
Genes de Plantas
Tubo Polínico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1001
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090120
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-008-0208-2


  10 / 17 MEDLINE  
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[PMID]:17205968
[Au] Autor:Hirai M; Kamimura T; Kanno A
[Ad] Endereço:Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi, Japan.
[Ti] Título:The expression patterns of three class B genes in two distinctive whorls of petaloid tepals in Alstroemeria ligtu.
[So] Source:Plant Cell Physiol;48(2):310-21, 2007 Feb.
[Is] ISSN:0032-0781
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Alstroemeria (Liliales) has two layers of petaloid tepals, in which the often spotted narrow inner tepals can be distinguished easily from the wider outer tepals. In order to explore this floral morphology in Alstroemeria, we investigated the tepal morphology and the expression patterns of three class B genes, whose homologs in eudicots have been shown previously to be involved in petal and stamen development. The two DEF-like genes (AlsDEFa and AlsDEFb) and the one GLO-like gene (AlsGLO) of Alstroemeria ligtu were isolated by rapid amplification of cDNA ends (RACE). Northern hybridization, reverse transcription-PCR (RT-PCR) and in situ hybridization analyses indicated that AlsDEFb and AlsGLO were expressed in whorls 1, 2 and 3 (outer tepals, inner tepals and stamens, respectively), whereas AlsDEFa expression was detected only in whorls 2 and 3. These results suggest that in A. ligtu, AlsDEFb and AlsGLO would participate in determining the organ identity of the two-layered petaloid tepals and stamens, which is in support of the modified ABC model. Additionally, the distinctive expression patterns of AlsDEFa and AlsDEFb might be related to morphological differences between the two-layered tepals.
[Mh] Termos MeSH primário: Alstroemeria/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
[Mh] Termos MeSH secundário: Northern Blotting
Flores
Hibridização In Situ
Dados de Sequência Molecular
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0705
[Cu] Atualização por classe:070214
[Lr] Data última revisão:
070214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070109
[St] Status:MEDLINE



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