Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.875.175 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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  1 / 4 MEDLINE  
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[PMID]:29078848
[Au] Autor:Budchart P; Khamwut A; Sinthuvanich C; Ratanapo S; Poovorawan Y; T-Thienprasert NP
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
[Ti] Título:Partially Purified Gloriosa superba Peptides Inhibit Colon Cancer Cell Viability by Inducing Apoptosis Through p53 Upregulation.
[So] Source:Am J Med Sci;354(4):423-429, 2017 Oct.
[Is] ISSN:1538-2990
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Colon cancer is a major health problem worldwide. Available treatments such as surgery, chemotherapy, radiation and anticancer drugs are limited due to stage of cancer, side effects and altered biodistribution. The use of peptides extracted from natural products has appeared as a potential therapy. Gloriosa superba is known to contain colchicine and other alkaloids with anticancer activity. However, these peptides contained within the extracts have not been studied. This study, therefore, focuses on an investigation of anti-colon cancer activity from a partially purified protein hydrolysate of G superba rhizome. METHODS: Dried G superba rhizome was extracted using 0.5% sodium dodecyl sulfate and digested with pepsin. The protein hydrolysates with molecular weight lesser than 3kDa were collected and subjected for cell viability assay. Then, the partial purification of the protein hydrolysate was performed using reverse-phase high-performance liquid chromatography. Fractions containing anticancer peptides were investigated, and their effects on apoptosis and protein expression using apoptosis test and Western blot, respectively. RESULTS: Partially purified peptides of G superba rhizome demonstrated anticolon activity in SW620 cells by inducing apoptosis through upregulation of p53 and downregulation of nuclear factor kappa B (NF-κB). CONCLUSIONS: Consequently, G superba peptides showed high potential for further purification and development of anticolon therapeutics.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Colchicaceae/química
Neoplasias do Colo/tratamento farmacológico
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Peptídeos/farmacologia
Proteínas de Plantas/farmacologia
Rizoma/química
Proteína Supressora de Tumor p53/biossíntese
Regulação para Cima/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Cercopithecus aethiops
Neoplasias do Colo/metabolismo
Neoplasias do Colo/patologia
Seres Humanos
Peptídeos/química
Proteínas de Plantas/química
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Peptides); 0 (Plant Proteins); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171029
[St] Status:MEDLINE


  2 / 4 MEDLINE  
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[PMID]:28211687
[Au] Autor:Zarev Y; Foubert K; Ionkova I; Apers S; Pieters L
[Ad] Endereço:Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
[Ti] Título:Isolation and Structure Elucidation of Glucosylated Colchicinoids from the Seeds of Gloriosa superba by LC-DAD-SPE-NMR.
[So] Source:J Nat Prod;80(4):1187-1191, 2017 Apr 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Four new colchicinoids were isolated from the seeds of Gloriosa superba together with the known compounds colchicoside (4) and 3-de-O-methylcolchicine-3-O-ß-d-glucopyranosyl-(1→4)-3-O-ß-d-glucopyranoside (6), by means of conventional column chromatography and LC-DAD-SPE-NMR. The new compounds were identified as N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-ß-d-glucopyranoside (1), 3-de-O-methylcolchicine-3-O-ß-d-glucopyranosyl-(1→6)-3-O-ß-d-glucopyranoside (2), N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-ß-d-glucopyranosyl-(1→6)-3-O-ß-d-glucopyranoside (3), and 3-de-O-methylcolchicine-3-O-ß-d-glucopyranosyl-(1→3)-3-O-ß-d-glucopyranoside (5). The structure elucidation was performed by means of NMR (COSY, HSQC, and HMBC), HRESIMS/MS, and GCMS data analysis.
[Mh] Termos MeSH primário: Colchicaceae/química
Colchicina/análogos & derivados
Colchicina/isolamento & purificação
Glicosídeos/isolamento & purificação
Liliaceae/química
Saponinas/isolamento & purificação
Sementes/química
[Mh] Termos MeSH secundário: Colchicina/química
Glicosídeos/química
Índia
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Saponins); SML2Y3J35T (Colchicine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01024


  3 / 4 MEDLINE  
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[PMID]:27765621
[Au] Autor:Gopinath K; Kumaraguru S; Bhakyaraj K; Mohan S; Venkatesh KS; Esakkirajan M; Kaleeswarran P; Alharbi NS; Kadaikunnan S; Govindarajan M; Benelli G; Arumugam A
[Ad] Endereço:Department of Nanoscience and Technology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
[Ti] Título:Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities.
[So] Source:Microb Pathog;101:1-11, 2016 Dec.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The green fabrication of metal nanoparticles using botanical extracts is gaining increasing research attention in nanotechnology, since it does not require high energy inputs or the production of highly toxic chemical byproducts. Here, silver (Ag), gold (Au) and their bimetallic (Ag/Au) nanoparticles (NPs) were green synthesized using the Gloriosa superba aqueous leaf extract. Metal NPs were studied by spectroscopic (UV-visible spectroscopy, fluorescence spectroscopy, FT-IR spectroscopy, XRD and EDX) and microscopic (AFM and TEM) analysis. AFM and TEM showed that Ag and Au NPs had triangular and spherical morphologies, with an average size of 20 nm. Bimetallic Ag/Au NPs showed spherical shapes with an average size of 10 nm. Ag and Ag/Au bimetallic NPs showed high antibacterial and antibiofilm activities towards Gram-positive and Gram-negative bacteria. Overall, the proposed synthesis route of Ag, Au and Ag/Au bimetallic NPs can be exploited by the pharmaceutical industry to develop drugs effective in the fight against microbic infections.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Colchicaceae/química
Ouro/metabolismo
Química Verde/métodos
Nanopartículas Metálicas/química
Extratos Vegetais/metabolismo
Prata/metabolismo
[Mh] Termos MeSH secundário: Bactérias/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Nanopartículas Metálicas/ultraestrutura
Microscopia de Força Atômica
Microscopia Eletrônica de Transmissão
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 3M4G523W1G (Silver); 7440-57-5 (Gold)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


  4 / 4 MEDLINE  
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[PMID]:27603689
[Au] Autor:Kumar R; Gupta YK; Singh S; Patil A
[Ad] Endereço:a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
[Ti] Título:Glorisa superba Hydroalcoholic Extract from Tubers Attenuates Experimental Arthritis by Downregulating Inflammatory Mediators, and Phosphorylation of ERK/JNK/p-38.
[So] Source:Immunol Invest;45(7):603-18, 2016 Oct.
[Is] ISSN:1532-4311
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glorisa superba (GS) is a medicinal plant that has been traditionally used in the treatment of joint pain and rheumatoid arthritis (RA). The present study was carried out to investigate the antiarthritic activity of Glorisa superba hydroalcoholic extract (GSHE) in an adjuvant-induced arthritis (AIA) rat model. Arthritis was induced by sub-plantar administration of complete Freund's adjuvant (CFA) and GSHE (25, 50, or 100 mg/kg/day) was administered orally for 21 consecutive days. Joint diameter was measured on Days 0, 3, 7, 14, and 21. GSHE dose dependently attenuates the increased joint diameter and serum tumor necrosis factor (TNF)-α level following induction of arthritis by adjuvant. This attenuation was well substantiated with reduced mRNA expression of interleukin (IL)-1ß, IL-6, TNF-α, and NF-κB. Additionally, GSHE inhibited phosphorylation of the mitogen-activated protein kinases (MAPK) signaling pathway as there was decreased protein expression of MAPK (p-p38/p38 and p-ERK/ERK p-JNK/JNK ratio). Moreover, GSHE in a dose-dependent fashion normalized the redox status of ankle joint (GSH, malonaldialdehyde [MDA], and NO levels and superoxide dismutase [SOD] and catalase [CAT] activities) and displayed decreased inflammatory cell infiltration in histopathological findings. Taken together, these findings indicate that GSHE protects against AIA by modulating MAPK.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Artrite Experimental/tratamento farmacológico
Artrite Reumatoide/tratamento farmacológico
Colchicaceae/imunologia
Articulações/efeitos dos fármacos
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Citocinas/genética
Citocinas/metabolismo
Regulação para Baixo
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Seres Humanos
Mediadores da Inflamação/metabolismo
Articulações/patologia
Articulações/fisiologia
MAP Quinase Quinase 4/metabolismo
Masculino
Modelos Animais
NF-kappa B/genética
NF-kappa B/metabolismo
Fosforilação Oxidativa/efeitos dos fármacos
Ratos
Ratos Wistar
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Inflammation Mediators); 0 (NF-kappa B); 0 (Plant Extracts); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.12.2 (MAP Kinase Kinase 4)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1080/08820139.2016.1195406



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