Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.875.625.860 [Categoria DeCS]
Referências encontradas : 51 [refinar]
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[PMID]:28454671
[Au] Autor:Qin XJ; Si YA; Chen Y; Liu H; Ni W; Yan H; Shu T; Ji YH; Liu HY
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, People's Republic of China.
[Ti] Título:Cytotoxic steroidal saponins from Trillium kamtschaticum.
[So] Source:Bioorg Med Chem Lett;27(11):2267-2273, 2017 06 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eight new steroidal saponins, trillikamtosides K-R (1-8), along with three known analogues, were isolated from the whole plants of Trillium kamtschaticum. Their structures were unambiguously established by interpretation of spectroscopic data (MS and NMR) and chemical methods. Compound 1 had a rare aglycone featuring a skeleton of 16-oxaandrost-5-en-3-ol-17-one, which was reported for the first time. The isolated saponins were tested for cytotoxicities against HCT116 cells, and trillikamtoside R (8) was found to show the most cytotoxic effect with an IC value of 4.92µM.
[Mh] Termos MeSH primário: Saponinas/isolamento & purificação
Trillium/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Células HCT116
Seres Humanos
Concentração Inibidora 50
Espectroscopia de Prótons por Ressonância Magnética
Saponinas/farmacologia
Espectrometria de Massas por Ionização por Electrospray
Esteroides/isolamento & purificação
Esteroides/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Saponins); 0 (Steroids)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:27693742
[Au] Autor:Yan T; Yu X; Sun X; Meng D; Jia JM
[Ad] Endereço:School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China.
[Ti] Título:A new steroidal saponin, furotrilliumoside from Trillium tschonoskii inhibits lipopolysaccharide-induced inflammation in Raw264.7 cells by targeting PI3K/Akt, MARK and Nrf2/HO-1 pathways.
[So] Source:Fitoterapia;115:37-45, 2016 Dec.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new steroidal saponin, furotrilliumoside (FT) was isolated from the roots and rhizomes of Trillium tschonoskii Maxim. Its structure was elucidated on the basis of 1D- and 2D-NMR spectroscopic data as well as HR-ESI-MS analysis. FT showed superior activity of inhibiting NO production of RAW264.7 cells induced by lipopolysaccharide (LPS) in the preliminary biological screening. In order to develop novel therapeutic drug for acute and chronic inflammatory disorders, the anti-inflammatory activity and underlying mechanism of FT were investigated in LPS-induced RAW264.7 cells. The results showed that FT could reduce LPS-induced expression of inducible nitric oxide synthase (iNOS) and then resulted in the decrement of NO production. More meaningful, FT could down-regulate the expression of cyclooxygenase-2 (COX-2) and decrease the expressions of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1ß (IL-1ß), in both gene and protein levels. In mechanism study, FT blocked the LPS-induced upregulation of phosphorylated phosphoinositide-3-kinase and Akt (PI3K/Akt). Furthermore, FT inhibited the translocation of nuclear factor-kappa B (NF-κB) through the prevention of inhibitory factor kappa B alpha (IκBα) phosphorylation and degradation and also suppressed the mitogen-activated protein kinases (MAPK) signaling pathway in LPS-stimulated RAW264.7 macrophages. In addition, FT upregulated heme oxygenase-1 (HO-1) expression via nuclear translocation of nuclear factor E2-related factor 2 (Nrf2). Taken together, FT might act as a natural agent to treat some inflammatory diseases by targeting PI3K/Akt, MARK and Nrf2/HO-1 pathways.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Saponinas/química
Transdução de Sinais/efeitos dos fármacos
Trillium/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Ciclo-Oxigenase 2/metabolismo
Heme Oxigenase-1/metabolismo
Interleucina-1beta/metabolismo
Interleucina-6/metabolismo
Lipopolissacarídeos
Proteínas de Membrana/metabolismo
Camundongos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Fator 2 Relacionado a NF-E2/metabolismo
NF-kappa B/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Raízes de Plantas/química
Proteínas Proto-Oncogênicas c-akt/metabolismo
Células RAW 264.7
Rizoma/química
Saponinas/isolamento & purificação
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Membrane Proteins); 0 (NF-E2-Related Factor 2); 0 (NF-kappa B); 0 (Nfe2l2 protein, mouse); 0 (Saponins); 0 (Tumor Necrosis Factor-alpha); 0 (furotrilliumoside); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse); EC 1.14.14.18 (Heme Oxygenase-1); EC 1.14.14.18 (Hmox1 protein, mouse); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27665149
[Au] Autor:Chen Y; Ni W; Yan H; Qin XJ; Khan A; Liu H; Shu T; Jin LY; Liu HY
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100039, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, 650201, China.
[Ti] Título:Spirostanol glycosides with hemostatic and antimicrobial activities from Trillium kamtschaticum.
[So] Source:Phytochemistry;131:165-173, 2016 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ten spirostanol glycosides, trillikamtosides A-J, together with eleven known analogues, were isolated from the hemostatic fraction of the 75% aqueous EtOH extract of the whole herbs of Trillium kamtschaticum. Their structures were established by extensive spectroscopic data analysis and chemical methods. The aglycones of three of these compounds had unique 3ß,17α-dihydroxy-spirostanes featuring a double bond between C-4 and C-5, while two others represent a rare class of spirostanol glycosides which possess a 5(6 â†’ 7) abeo-steroidal aglycone. All the compounds were evaluated for their hemostatic and antimicrobial activities. Three of the spirostanol glycosides exhibited induced-platelet aggregation at a concentration of 300 µg/mL with maximal induced-platelet aggregation rates of 72%, 71%, and 62% in rabbits, respectively, and their EC values were 492.7, 203.3, and 109.8 µM. Five of the spirostanol glycosides showed an anti-Candida albicans effect with MIC values of 21.1, 10.6, 8.8, 21.6, and 11.0 µM, respectively.
[Mh] Termos MeSH primário: Anti-Infecciosos/isolamento & purificação
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Hemostáticos/isolamento & purificação
Hemostáticos/farmacologia
Espirostanos/isolamento & purificação
Espirostanos/farmacologia
Trillium/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Candida albicans/efeitos dos fármacos
Relação Dose-Resposta a Droga
Glicosídeos/química
Hemostáticos/química
Agregação Plaquetária/efeitos dos fármacos
Espirostanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Glycosides); 0 (Hemostatics); 0 (Spirostans)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160926
[St] Status:MEDLINE


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[PMID]:27598942
[Au] Autor:Wu H; Qiu Y; Shu Z; Zhang X; Li R; Liu S; Chen L; Liu H; Chen N
[Ad] Endereço:a College of Science and Technology of Hubei University for Nationalities, Enshi 445000, China.
[Ti] Título:Protective effect of Trillium tschonoskii saponin on CCl -induced acute liver injury of rats through apoptosis inhibition.
[So] Source:Can J Physiol Pharmacol;94(12):1291-1297, 2016 Dec.
[Is] ISSN:1205-7541
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:To explore hepatoprotective role and underlying mechanisms of Trillium tschonoskii Maxim (TTM), 36 rats were randomly divided into control, CCl -induced liver injury model, and biphenyl dimethyl dicarboxylate (DDB) and low-, moderate-, and high-dose TTM treatment groups. After CCl -induced model establishment, the rats from DDB and TTM groups were administrated with DDB at 0.2 g/kg per day and TTM at 0.1, 0.5, and 1.0 g/kg per day, while the rats from control and model groups were administrated with saline. After 5 days of treatments, all rats were sacrificed for determining serum ALT and AST levels and liver index, examining histopathological changes in liver through HE and TUNEL staining, and evaluating TNF-α and IL-6 mRNA expression by real-time PCR, and caspase-3, Bcl-2, and Bax expression by Western blot. Results indicated that CCl could induce acute liver injury and abnormal liver function in rats with obvious hepatomegaly, increased liver index, high ALT and AST levels, up-regulated TNF-α and IL-6, and overexpressed Bax and caspase-3. However, DDB and TTM could execute protective role in CCl -induced liver injury in rats through reducing ALT and AST levels, rescuing hepatomegaly, down-regulating inflammatory factors and inhibiting hepatocyte apoptosis in a dose-dependent manner. Therefore, TTM has obvious protective role in CCl -induced liver injury of rats through inhibiting hepatocyte apoptosis.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Tetracloreto de Carbono/toxicidade
Doença Hepática Induzida por Substâncias e Drogas/patologia
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Saponinas/uso terapêutico
Trillium
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Masculino
Substâncias Protetoras/isolamento & purificação
Substâncias Protetoras/farmacologia
Substâncias Protetoras/uso terapêutico
Ratos
Saponinas/isolamento & purificação
Saponinas/farmacologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protective Agents); 0 (Saponins); CL2T97X0V0 (Carbon Tetrachloride)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170210
[Lr] Data última revisão:
170210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE


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[PMID]:27572907
[Au] Autor:Cheng G; Gao F; Sun X; Bi H; Zhu Y
[Ad] Endereço:Department of Sports Medicine, Yantaishan Hospital, Yantai, Shandong 264100, P.R. China.
[Ti] Título:Paris saponin VII suppresses osteosarcoma cell migration and invasion by inhibiting MMP­2/9 production via the p38 MAPK signaling pathway.
[So] Source:Mol Med Rep;14(4):3199-205, 2016 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Metastasis is the primary cause of mortality in osteosarcoma. Targeting metastasis is a major strategy in osteosarcoma treatment. As a traditional Chinese medicine, Trillium tschonoskii Maxim has been widely used in the therapy of various diseases, including cancer. However, currently there is no evidence regarding the anti­metastasic effect of Paris saponin VII (PS VII), which is extracted from Trillium tschonoskii Maxim, on osteosarcoma cells and its underling mechanisms. The present study aimed to examine the effect of PS VII on the migration and invasion of osteosarcoma cells. Viability and proliferation of osteosarcoma cells were examined by MTT assay. Migration and invasion of osteosarcoma cells was then detected using scratch wound healing assays and Transwell assays, respectively. Additionally, the expression of matrix metalloproteinase (MMP)­2 and ­9 was determined at the mRNA and protein level following treatment with PS VII. Mitogen­activated protein kinase (MAPK) expression was also detected by western blot analysis. Finally, an inhibitor of p38 MAPK was used to verify the effect of PS VII on the expression of MMP­2 and ­9, as well as the migration and invasion osteosarcoma cells. This demonstrated that the proliferation, migration and invasion of the osteosarcoma cells were suppressed following treatment with PS VII. PS VII downregulated the expression of MMP­2 and ­9 in a dose­ and time­dependent manner. PS VII also exerted its ability to downregulate the phosphorylation of p38 MAPKs. Furthermore, by using a p38 inhibitor, SB203580, the role of PS VII in MMP­2 and ­9 expression and osteosarcoma cell invasion was revealed. Taken together, these results demonstrated that PS VII suppresses the migration and invasion of osteosarcoma cells via the p38 MAPK signaling pathway.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Neoplasias Ósseas/tratamento farmacológico
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Inibidores de Metaloproteinases de Matriz/farmacologia
Invasividade Neoplásica/prevenção & controle
Osteossarcoma/tratamento farmacológico
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Neoplasias Ósseas/metabolismo
Neoplasias Ósseas/patologia
Osso e Ossos/efeitos dos fármacos
Osso e Ossos/metabolismo
Osso e Ossos/patologia
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Seres Humanos
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Invasividade Neoplásica/patologia
Osteossarcoma/metabolismo
Osteossarcoma/patologia
Saponinas/química
Trillium/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Matrix Metalloproteinase Inhibitors); 0 (Paris saponin VII); 0 (Saponins); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5663


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[PMID]:27556434
[Au] Autor:Ur Rahman S; Adhikari A; Ismail M; Raza Shah M; Khurram M; Shahid M; Ali F; Haseeb A; Akbar F; Iriti M
[Ad] Endereço:Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal, Dir (U)-18000, Pakistan. shafiq@sbbu.edu.pk.
[Ti] Título:Beneficial Effects of Trillium govanianum Rhizomes in Pain and Inflammation.
[So] Source:Molecules;21(8), 2016 Aug 20.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Trillium govanianum rhizome is used as an analgesic and anti-inflammatory remedy in traditional medicine in northern Pakistan. In an attempt to establish its medicinal value, the present research evaluated the analgesic and anti-inflammatory potential of T. govanianum. The in vivo anti-inflammatory activity of extract and fractions was investigated in the carrageenan induced paw edema assay. The in vitro suppression of oxidative burst of extract, fractions and isolated compounds was assessed through luminol-enhanced chemiluminescence assay. The in vivo analgesic activity was assayed in chemical and thermal induced nociceptive pain models. The crude methanol extract and its solvent fractions showed anti-inflammatory and analgesic responses, exhibited by significant amelioration of paw edema and relieve of the tonic visceral chemical and acute phasic thermal nociception. In the oxidative burst assay, based on IC50, the crude methanol extract and n-butanol soluble fraction produced a significant inhibition, followed by chloroform and hexane soluble fractions as compared to ibuprofen. Similarly, the isolated compounds pennogenin and borassoside E exhibited significant level of oxidative burst suppressive activity. The in vivo anti-inflammatory and analgesic activities as well as the in vitro inhibition of oxidative burst validated the traditional use of T. govanianum rhizomes as a phytotherapeutic remedy for both inflammatory conditions and pain. The observed activities might be attributed to the presence of steroids and steroid-based compounds. Therefore, the rhizomes of this plant species could serve as potential novel source of compounds effective for alleviating pain and inflammation.
[Mh] Termos MeSH primário: Analgésicos/administração & dosagem
Anti-Inflamatórios/administração & dosagem
Edema/tratamento farmacológico
Dor Nociceptiva/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Rizoma/química
Trillium/química
[Mh] Termos MeSH secundário: Analgésicos/química
Analgésicos/farmacologia
Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Carragenina/efeitos adversos
Modelos Animais de Doenças
Edema/induzido quimicamente
Etanol/administração & dosagem
Etanol/isolamento & purificação
Etanol/farmacologia
Medicina Tradicional
Camundongos
Células NIH 3T3
Estresse Oxidativo/efeitos dos fármacos
Paquistão
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Saponinas/administração & dosagem
Saponinas/isolamento & purificação
Saponinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 0 (Saponins); 3K9958V90M (Ethanol); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE


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[PMID]:27364153
[Au] Autor:Khan KM; Nahar L; Al-Groshi A; Zavoianu AG; Evans A; Dempster NM; Wansi JD; Ismail FM; Mannan A; Sarker SD
[Ad] Endereço:Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, UK.
[Ti] Título:Cytotoxicity of the Roots of Trillium govanianum Against Breast (MCF7), Liver (HepG2), Lung (A549) and Urinary Bladder (EJ138) Carcinoma Cells.
[So] Source:Phytother Res;30(10):1716-1720, 2016 Oct.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Trillium govanianum Wall. (Melanthiaceae alt. Trilliaceae), commonly known as 'nag chhatri' or 'teen patra', is a native species of the Himalayas. It is used in various traditional medicines containing both steroids and sex hormones. In folk medicine, the rhizomes of T. govanianum are used to treat boils, dysentery, inflammation, menstrual and sexual disorders, as an antiseptic and in wound healing. With the only exception of the recent report on the isolation of a new steroidal saponin, govanoside A, together with three known steroidal compounds with antifungal property from this plant, there has been no systematic pharmacological and phytochemical work performed on T. govanianum. This paper reports, for the first time, on the cytotoxicity of the methanol extract of the roots of T. govanianum and its solid-phase extraction (SPE) fractions against four human carcinoma cell lines: breast (MCF7), liver (HEPG2), lung (A549) and urinary bladder (EJ138), using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide cytotoxicity assay and liquid chromatography and electrospray ionization quadrupole time-of-flight mass spectrometry analysis of the SPE fractions. The methanol extract and all SPE fractions exhibited considerable levels of cytotoxicity against all cell lines, with the IC values ranging between 5 and 16 µg/mL. Like other Trillium species, presence of saponins and sapogenins in the SPE fractions was evident in the liquid chromatography mass spectrometry data. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Mama/efeitos dos fármacos
Fígado/efeitos dos fármacos
Pulmão/efeitos dos fármacos
Extratos Vegetais/química
Raízes de Plantas/química
Trillium/química
Bexiga Urinária/efeitos dos fármacos
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5672


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[PMID]:26706077
[Au] Autor:Kubota S; Kanno A
[Ad] Endereço:Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. Electronic address: skubota44@gmail.com.
[Ti] Título:Analysis of the floral MADS-box genes from monocotyledonous Trilliaceae species indicates the involvement of SEPALLATA3-like genes in sepal-petal differentiation.
[So] Source:Plant Sci;241:266-76, 2015 Dec.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The evolution of greenish sepals from petaloid outer tepals has occurred repeatedly in various lineages of non-grass monocots. Studies in distinct monocot species showed that the evolution of sepals could be explained by the ABC model; for example, the defect of B-class function in the outermost whorl was linked to the evolution of sepals. Here, floral MADS-box genes from three sepal-bearing monocotyledonous Trilliaceae species, Trillium camschatcense, Paris verticillata, and Kinugasa japonica were examined. Unexpectedly, expression of not only A- but also B-class genes was detected in the sepals of all three species. Although the E-class gene is generally expressed across all floral whorls, no expression was detected in sepals in the three species examined here. Overexpression of the E-class SEPALLATA3-like gene from T. camschatcense (TcamSEP) in Arabidopsis thaliana produced phenotypes identical to those reported for orthologs in other monocots. Additionally, yeast hybrid experiments indicated that TcamSEP could form a higher-order complex with an endogenous heterodimer of B-class APETALA3/DEFICIENS-like (TcamDEF) and PISTILLATA/GLOBOSA-like (TcamGLO) proteins. These results suggest a conserved role for Trilliaceae SEPALLATA3-like genes in functionalization of the B-class genes, and that a lack of SEPALLATA3-like gene expression in the outermost whorl may be related to the formation of greenish sepals.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
Liliaceae/genética
Proteínas de Domínio MADS/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Evolução Molecular
Flores/genética
Flores/crescimento & desenvolvimento
Liliaceae/crescimento & desenvolvimento
Liliaceae/metabolismo
Proteínas de Domínio MADS/metabolismo
Filogenia
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/metabolismo
Alinhamento de Sequência
Trillium/genética
Trillium/crescimento & desenvolvimento
Trillium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MADS Domain Proteins); 0 (Plant Proteins)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151227
[St] Status:MEDLINE


  9 / 51 MEDLINE  
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[PMID]:26697697
[Au] Autor:Zhou WB; Lin L; Li ZY; Bi T; Ye TY; Ma CQ; Bao Hong-juan; Wang HP; Zhang BX; Song KK; Li YW; Wang Y
[Ti] Título:Study on active ingredient and mechanism in preventing vascular dementia of Tianzhusan coming from Tujia medicine.
[So] Source:Zhongguo Zhong Yao Za Zhi;40(13):2668-73, 2015 Jul.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:To make clear of the absorbed components of Tianzhusan (TZS) and its possible mechanism in preventing vascular dementia (VD), the rats' models of VD were prepared by a permanent ligation of the bilateral common carotid arteries. After 60 days, rats were administrated with TZS for 0.1 g x kg(-1), and the volume is 0.02 mL x g(-1). After 3 days, the medicated serum was prepared and detected by UPLC, and then we predicted the possible chemical structure of the absorbed components of TZS. According to the absorbed components, the potential targets of TZS were found by ligand profiling of Discovery Studio 3.5. All of these target genes were submitted to DAVID onine for gene set enrichment analysis (GSEA). The 5 absorbed components of TZS have been predicted, and four of them have been identified as parishin B, parishin C, parishin, pennogenin-3-O-alpha-L-rhamnopyranosy-(1-->2)-beta-D-glucoside. Through reverse finding targets, we got 861 pharmacophore models and 9 pathways from KEGG, BIOCARTA after document verification. These results showed that the efficacy mechanism of TZS on VD perhaps were be related with these absorbed components and pathways. If the traditional herbs could be proved effective by efficacy tests, the serum pharmacochemistry, computer-aided drug design, system biology and other technologies can be used in the next experiments, which will be beneficial to fast discovery of material basis and mechanisms of traditional medicine coming form ethnic minorities.
[Mh] Termos MeSH primário: Demência Vascular/prevenção & controle
Medicamentos de Ervas Chinesas/uso terapêutico
Gastrodia/química
Medicina Tradicional Chinesa
Trillium/química
[Mh] Termos MeSH secundário: Animais
Descoberta de Drogas
Masculino
Ratos
Ratos Sprague-Dawley
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:151224
[Lr] Data última revisão:
151224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151225
[St] Status:MEDLINE


  10 / 51 MEDLINE  
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[PMID]:26505320
[Au] Autor:Shafiq-ur-Rahman; Ismail M; Shah MR; Adhikari A; Anis I; Ahmad MS; Khurram M
[Ad] Endereço:Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan; Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal, Dir (U) 18000, Pakistan; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi
[Ti] Título:Govanoside A, a new steroidal saponin from rhizomes of Trillium govanianum.
[So] Source:Steroids;104:270-5, 2015 Dec.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new spirostane steroidal saponin, govanoside A (1) along with three known compounds borassoside E (2) pennogenin (3) and diosgenin (4) were isolated from rhizomes of Trillium govanianum. Their structures were elucidated through 1D, 2D-NMR spectroscopic data analysis and acid hydrolysis. Compound (2) in genus Trillium and all compounds (1-4) in T. govanianum are reported herein for the first time. Furthermore, compounds 1 &2 exhibited good to moderate activities against Aspergillus niger ATCC 16888, Aspergillus flavus ATCC 9643, Candida albicans ATCC 18804, and Candida glabrata ATCC 90030. This is a significant finding keeping in view the limited antifungal drugs for aspergillosis and candidiasis.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Aspergillus/efeitos dos fármacos
Candida/efeitos dos fármacos
Rizoma/química
Saponinas/farmacologia
Trillium/química
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/isolamento & purificação
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Conformação Molecular
Saponinas/química
Saponinas/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Saponins); 0 (govanoside A)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151028
[St] Status:MEDLINE



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