Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.875.750.500 [Categoria DeCS]
Referências encontradas : 171 [refinar]
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  1 / 171 MEDLINE  
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[PMID]:28368360
[Au] Autor:Zhong C; Hu D; Hou LB; Song LY; Zhang YJ; Xie Y; Tian LW
[Ad] Endereço:School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. vanbenchat@126.com.
[Ti] Título:Phenolic Compounds from the Rhizomes of Smilax china L. and Their Anti-Inflammatory Activity.
[So] Source:Molecules;22(4), 2017 Apr 03.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A new triflavanoid, kandelin B-5 ( ), was isolated from the rhizomes of L., together with six known phenylpropanoid substituted flavan-3-ols ( - ), nine flavonoids ( - ), two stilbenoids ( , ), and two other compounds ( , ). The structure of compound was determined on the basis of 1D, 2D NMR and HR-ESI-MS data, as well as chemical method. Compounds - , - , , , and were evaluated for anti-inflammatory activity. Only compounds , and showed slightly IL-1ß expression inhibitory activities on LPS induced THP-1 cells, with inhibition rate of 15.8%, 37.3%, and 35.8%, respectively, at concentration of 50 µg/mL.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Flavonoides/farmacologia
Extratos Vegetais/farmacologia
Rizoma/química
Smilax/química
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Linhagem Celular
Citocinas/biossíntese
Flavonoides/química
Flavonoides/isolamento & purificação
Seres Humanos
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


  2 / 171 MEDLINE  
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[PMID]:28287485
[Au] Autor:Lee HE; Kim JA; Whang WK
[Ad] Endereço:Pharmaceutical Botany Laboratory, College of Pharmacy, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea. y-u-h-r214@hanmail.net.
[Ti] Título:Chemical Constituents of Smilax china L. Stems and Their Inhibitory Activities against Glycation, Aldose Reductase, α-Glucosidase, and Lipase.
[So] Source:Molecules;22(3), 2017 Mar 11.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The search for natural inhibitors with anti-diabetes properties has gained increasing attention. Among four selected Smilacaceae family plants, L. stems (SCS) showed significant in vitro anti-glycation and rat lens aldose reductase inhibitory activities. Bioactivity-guided isolation was performed with SCS and four solvent fractions were obtained, which in turn yielded 10 compounds, including one phenolic acid, three chlorogenic acids, four flavonoids, one stilbene, and one phenylpropanoid glycoside; their structures were elucidated using nuclear magnetic resonance and mass spectrometry. All solvent fractions, isolated compounds, and stem extracts from plants sourced from six different provinces of South Korea were next tested for their inhibitory effects against advanced glycation end products, as well as aldose reductase. α-Glucosidase, and lipase assays were also performed on the fractions and compounds. Since compounds , , , and appeared to be the superior inhibitors among the tested compounds, a comparative study was performed via high-performance liquid chromatography with photodiode array detection using a self-developed analysis method to confirm the relationship between the quantity and bioactivity of the compounds in each extract. The findings of this study demonstrate the potent therapeutic efficacy of SCS and its potential use as a cost-effective natural alternative medicine against type 2 diabetes and its complications.
[Mh] Termos MeSH primário: Aldeído Redutase/antagonistas & inibidores
Ácido Clorogênico/química
Produtos Finais de Glicação Avançada/antagonistas & inibidores
Glicosídeos/química
Hidroxibenzoatos/química
Smilax/química
Estilbenos/química
[Mh] Termos MeSH secundário: Aldeído Redutase/metabolismo
Animais
Ácido Clorogênico/isolamento & purificação
Ácido Clorogênico/farmacologia
Etanol/química
Flavonoides/química
Flavonoides/isolamento & purificação
Flavonoides/farmacologia
Produtos Finais de Glicação Avançada/metabolismo
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Glicosilação/efeitos dos fármacos
Hidroxibenzoatos/isolamento & purificação
Hidroxibenzoatos/farmacologia
Cristalino/química
Cristalino/enzimologia
Lipase/antagonistas & inibidores
Lipase/metabolismo
Metanol/química
Estrutura Molecular
Extratos Vegetais/química
Caules de Planta/química
Ratos
Ratos Sprague-Dawley
Solventes/química
Estilbenos/isolamento & purificação
Estilbenos/farmacologia
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Glycation End Products, Advanced); 0 (Glycosides); 0 (Hydroxybenzoates); 0 (Plant Extracts); 0 (Solvents); 0 (Stilbenes); 29656-58-4 (phenolic acid); 318ADP12RI (Chlorogenic Acid); 3K9958V90M (Ethanol); EC 1.1.1.21 (Aldehyde Reductase); EC 3.1.1.3 (Lipase); EC 3.2.1.20 (alpha-Glucosidases); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


  3 / 171 MEDLINE  
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[PMID]:28276765
[Au] Autor:Liu X; Liang J; Pan LL; Chen JY; Liu RH; Zhu GH; Huang HL; Shu JC; Shao F; Liang YH; Yu JL
[Ad] Endereço:a Key Laboratory of Modern Preparation of TCM , Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang 330004 , China.
[Ti] Título:Six new furostanol glycosides from Smilax glauco-china and their cytotoxic activity.
[So] Source:J Asian Nat Prod Res;19(8):754-765, 2017 Aug.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Six new steroidal saponins, namely glauco-chinaosides A-F, and one known compound were isolated from the tubers of Smilax glauco-china. Their structures were elucidated by a combination of spectroscopic analysis and hydrolysis followed by spectral and chromatographic analysis. Compounds 1-7 were tested in vitro for their cytotoxic activities against four human tumor cell lines (SH-SY5Y, SGC-7901, HCT-116, and Lovo). Compounds 1, 2, and 5 exhibited cytotoxic activity against SGC-7901, with IC values of 2.7, 11.5, and 6.8 µM, respectively.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Saponinas/isolamento & purificação
Saponinas/farmacologia
Smilax/química
Esteróis/isolamento & purificação
Esteróis/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Glicosídeos/química
Células HCT116
Seres Humanos
Concentração Inibidora 50
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Rizoma/química
Saponinas/química
Esteróis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (Glycosides); 0 (Saponins); 0 (Sterols); 0 (furostanol glycoside)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2017.1281913


  4 / 171 MEDLINE  
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[PMID]:28231749
[Au] Autor:Fu S; Yang Y; Liu D; Luo Y; Ye X; Liu Y; Chen X; Wang S; Wu H; Wang Y; Hu Q; You P
[Ad] Endereço:* Key Laboratory of Resources and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
[Ti] Título:Flavonoids and Tannins from Smilax china L. Rhizome Induce Apoptosis Via Mitochondrial Pathway and MDM2-p53 Signaling in Human Lung Adenocarcinoma Cells.
[So] Source:Am J Chin Med;45(2):369-384, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:In vitro evidence indicates that Smilax china L. rhizome (SCR) can inhibit cell proliferation. Therefore, in the present study, we analyzed the effects in vitro of SCR extracts on human lung adenocarcinoma A549 cells. Our results showed that A549 cell growth was inhibited in a dose- and time-dependent manner after treatment with SCR extracts. Total flavonoids and total tannins from SCR induced A549 apoptosis in a dose-dependent manner, as shown by our flow cytometry analysis, which was consistent with the alterations in nuclear morphology we observed. In addition, the total apoptotic rate induced by total tannins was higher than the rate induced by total flavonoids at the same dose. Cleaved-caspase-3 protein levels in A549 cells after treatment with total flavonoids or total tannins were increased in a dose-dependent manner, followed by the activation of caspase-8 and caspase-9, finally triggering to PARP cleavage. Furthermore, total flavonoids and total tannins increased the expression of Bax, decreased the expression of Bcl-2, and promoted cytochrome [Formula: see text] release. Moreover, MDM2 and p-MDM2 proteins were decreased, while p53 and p-p53 proteins were increased, both in a dose-dependent manner, after A549 treatment with total flavonoids and total tannins. Finally, cleaved-caspase-3 protein levels in the total flavonoids or total tannins-treated H1299 (p53 null) and p53-knockdown A549 cells were increased. Our results indicated that total flavonoids and total tannins from SCR exerted a remarkable effect in reducing A549 growth through their action on mitochondrial pathway and disruption of MDM2-p53 balance. Hence, our findings demonstrated a potential application of total flavonoids and total tannins from SCR in the treatment of human lung adenocarcinoma.
[Mh] Termos MeSH primário: Adenocarcinoma/patologia
Apoptose/efeitos dos fármacos
Flavonoides/farmacologia
Neoplasias Pulmonares/patologia
Mitocôndrias/metabolismo
Transdução de Sinais/efeitos dos fármacos
Smilax/química
Taninos/farmacologia
[Mh] Termos MeSH secundário: Adenocarcinoma/metabolismo
Caspases/metabolismo
Sobrevivência Celular
Relação Dose-Resposta a Droga
Flavonoides/isolamento & purificação
Seres Humanos
Neoplasias Pulmonares/metabolismo
Proteínas Proto-Oncogênicas c-mdm2/metabolismo
Taninos/isolamento & purificação
Células Tumorais Cultivadas
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (TP53 protein, human); 0 (Tannins); 0 (Tumor Suppressor Protein p53); EC 2.3.2.27 (MDM2 protein, human); EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500239


  5 / 171 MEDLINE  
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[PMID]:28094526
[Au] Autor:Chen T; Sheng J; Fu Y; Li M; Wang J; Jia AQ
[Ad] Endereço:School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Xiao Ling Wei No. 200, Nanjing 210094, China.
[Ti] Título:H NMR-Based Global Metabolic Studies of Pseudomonas aeruginosa upon Exposure of the Quorum Sensing Inhibitor Resveratrol.
[So] Source:J Proteome Res;16(2):824-830, 2017 Feb 03.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quorum sensing (QS) is a process of bacterial communication that has been a novel target for drug discovery. Pyocyanin quantification assay confirmed that resveratrol was an effective quorum sensing inhibitor (QSI) against Pseudomonas aeruginosa PAO1. In this study, the global metabolite changes of P. aeruginosa PAO1 exposed to QSI resveratrol were investigated by H NMR spectroscopy. A total of 40 metabolites containing amino acids, organic acid, organic amine, and energy storage compounds were identified. The changed metabolic profile indicated that resveratrol influenced pathways including oxidative stress, protein synthesis, and energy metabolism. Oxidative stress could upregulate the expression of genes related to QS in P. aeruginosa. It suggested that resveratrol could inhibit the QS systems in P. aeruginosa PAO1 by relieving oxidative stress due to its antioxidant activity. On the other hand, resveratrol could attenuate the pathogenicity of P. aeruginosa PAO1 by disturbing the TCA cycle so that anaerobic respiration could suppress the virulence because anaerobiosis could induce the loss of cytotoxicity regulated by QS in P. aeruginosa. These findings deepened our comprehending of the metabolic responses of P. aeruginosa PAO1 to resveratrol and pinpointed the possible underlying mechanism of resveratrol's inhibition effect on QS in P. aeruginosa PAO1.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Regulação Bacteriana da Expressão Gênica
Pseudomonas aeruginosa/efeitos dos fármacos
Percepção de Quorum/efeitos dos fármacos
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/isolamento & purificação
Ciclo do Ácido Cítrico/efeitos dos fármacos
Glicólise/efeitos dos fármacos
Redes e Vias Metabólicas/efeitos dos fármacos
Redes e Vias Metabólicas/genética
Metabolômica
Ressonância Magnética Nuclear Biomolecular
Estresse Oxidativo
Extratos Vegetais/química
Biossíntese de Proteínas/efeitos dos fármacos
Pseudomonas aeruginosa/genética
Pseudomonas aeruginosa/metabolismo
Piocianina/antagonistas & inibidores
Piocianina/biossíntese
Smilax/química
Estilbenos/isolamento & purificação
Fatores de Virulência/antagonistas & inibidores
Fatores de Virulência/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Stilbenes); 0 (Virulence Factors); 9OQM399341 (Pyocyanine); Q369O8926L (resveratrol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jproteome.6b00800


  6 / 171 MEDLINE  
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[PMID]:27370097
[Au] Autor:Li HG; Hou PY; Zhang X; He Y; Zhang J; Wang SQ; Anderson S; Zhang YW; Wu XH
[Ad] Endereço:Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
[Ti] Título:Hypouricemic effect of allopurinol are improved by Pallidifloside D based on the uric acid metabolism enzymes PRPS, HGPRT and PRPPAT.
[So] Source:Fitoterapia;113:1-5, 2016 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Allopurinol is a commonly used medication to treat hyperuricemia and its complications. Pallidifloside D, a saponin glycoside constituent from the total saponins of Smilax riparia, had been proved to enhanced hypouricemic effect of allopurinol based on uric acid metabolism enzyme XOD. In this study, we evaluated whether Pallidifloside D (5mg/kg) enhanced hypouricemic effect of allopurinol (5mg/kg) related to others uric acid metabolism enzymes such as PRPS, HGPRT and PRPPAT. We found that, compared with allopurinol alone, the combination of allopurinol and Pallidifloside D significantly up-regulated HGPRT mRNA expression and down-regulated the mRNA expression of PRPS and PRPPAT in PC12 cells (all P<0.01). These results strongly suggest that hypouricemic effect of allopurinol are improved by Pallidifloside D via numerous mechanisms and our data may have a potential value in clinical practice in the treatment of gout and other hyperuricemic conditions.
[Mh] Termos MeSH primário: Alopurinol/farmacologia
Hiperuricemia/tratamento farmacológico
Hipoxantina Fosforribosiltransferase/metabolismo
Ribose-Fosfato Pirofosfoquinase/metabolismo
Saponinas/farmacologia
Transaminases/metabolismo
[Mh] Termos MeSH secundário: Animais
Sinergismo Farmacológico
Regulação da Expressão Gênica/efeitos dos fármacos
Masculino
Camundongos
Células PC12
RNA Mensageiro/metabolismo
Ratos
Smilax/química
Ácido Úrico/sangue
Ácido Úrico/urina
Xantina Oxidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Saponins); 0 (pallidifloside D); 268B43MJ25 (Uric Acid); 63CZ7GJN5I (Allopurinol); EC 1.17.3.2 (Xanthine Oxidase); EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase); EC 2.6.1.- (Transaminases); EC 2.6.1.- (phosphoribosyl pyrophosphate aminotransferase); EC 2.7.6.1 (Ribose-Phosphate Pyrophosphokinase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE


  7 / 171 MEDLINE  
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[PMID]:27251500
[Au] Autor:Lincha VR; Zhao BT; Woo MH; Yang IJ; Shin HM
[Ad] Endereço:Department of Physiology, College of Korean Medicine, Dongguk University.
[Ti] Título:Effects of Constituent Compounds of Smilax china on Nicotine-Induced Endothelial Dysfunction in Human Umbilical Vein Endothelial Cells.
[So] Source:Biol Pharm Bull;39(6):984-92, 2016.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:This study investigated the effects of compounds isolated from 70% ethanol (EtOH) extraction of Smilax china L. (SCE), a plant belonging to the family Smilacaceae on nicotine-induced endothelial dysfunction (ED) in human umbilical vein endothelial cells. We isolated 10 compounds from ethyl acetate (EtOAc) fraction of 70% EtOH extract of SCE and investigated their inhibitory effect on nicotine-induced ED in endothelial cells. Kaempferol, kaempferol 7-O-α-L-rhamnopyranoside, puerarin and ferulic acid showed strong inhibition of nicotine-induced vascular cell adhesion molecule (VCAM-1) expression while kaempferol, kaempferin, and caffeic acid attenuated intercellular adhesion molecule (ICAM-1) expression. Lepidoside, caffeic acid and methylsuccinic acid caused the highest up-regulated expression of endothelial nitric oxide synthase at the protein level with caffeic acid and ferulic acid showing strong inhibitory effects on inducible nitric oxide synthase (iNOS) expression. In addition, ferulic acid and kaempferol showed inhibition against interleukin-8 (IL-8) and interleukin-1ß (IL-1ß) expression while ferulic acid and caffeic acid showed comparatively higher inhibition of ED associated tumor necrosis factor-α (TNF-α) expression. These results show the potential of the aforementioned compounds to reverse the toxic effects of nicotine on the endothelium.
[Mh] Termos MeSH primário: Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Nicotina/toxicidade
Extratos Vegetais/farmacologia
Smilax
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Citocinas/metabolismo
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Molécula 1 de Adesão Intercelular/metabolismo
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Óxido Nítrico Sintase Tipo III/metabolismo
Folhas de Planta
Molécula 1 de Adesão de Célula Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Plant Extracts); 0 (Vascular Cell Adhesion Molecule-1); 126547-89-5 (Intercellular Adhesion Molecule-1); 31C4KY9ESH (Nitric Oxide); 6M3C89ZY6R (Nicotine); EC 1.14.13.39 (NOS2 protein, human); EC 1.14.13.39 (NOS3 protein, human); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nitric Oxide Synthase Type III)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160603
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b15-00997


  8 / 171 MEDLINE  
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[PMID]:27111514
[Au] Autor:Kong G; Huang X; Wang L; Li Y; Sun T; Han S; Zhu W; Ma M; Xu H; Li J; Zhang X; Liu X; Wang X
[Ad] Endereço:Department of Intensive Care Unit Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China; Department of Biotechnology, Binzhou Medical University, Yantai 264003, Shandong Province, China.
[Ti] Título:Astilbin alleviates LPS-induced ARDS by suppressing MAPK signaling pathway and protecting pulmonary endothelial glycocalyx.
[So] Source:Int Immunopharmacol;36:51-58, 2016 Jul.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Acute respiratory distress syndrome (ARDS) is a devastating disorder that is characterized by increased vascular endothelial permeability and inflammation. Unfortunately, no effective treatment beyond supportive care is available for ARDS. Astilbin, a flavonoid compound isolated from Rhizoma Smilacis Glabrae, has been used for anti-hepatic, anti-arthritic, and anti-renal injury treatments. This study examined the effects of Astilbin on pulmonary inflammatory activation and endothelial cell barrier dysfunction caused by Gram-negative bacterial endotoxin lipopolysaccharide (LPS). Endothelial cells from human umbilical veins or male Kunming mice were pretreated with Astilbin 24h before LPS stimulation. Results showed that Astilbin significantly attenuated the pulmonary histopathological changes and neutrophil infiltration 6h after the LPS challenge. Astilbin suppressed the activities of myeloperoxidase and malondialdehyde, as well as the expression of tumor necrosis factor-α and interleukin-6 in vivo and in vitro. As indices of pulmonary edema, lung wet-to-dry weight ratios, were markedly decreased by Astilbin pretreatment. Western blot analysis also showed that Astilbin inhibited LPS-induced activation of mitogen-activated protein kinase (MAPK) pathways in lung tissues. Furthermore, Astilbin significantly inhibited the activity of heparanase and reduced the production of heparan sulfate in the blood serum as determined by ELISA. These findings indicated that Astilbin can alleviate LPS-induced ARDS, which potentially contributed to the suppression of MAPK pathway activation and the degradation of endothelial glycocalyx.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Células Endoteliais/efeitos dos fármacos
Flavonóis/uso terapêutico
Pulmão/efeitos dos fármacos
Síndrome do Desconforto Respiratório do Adulto/tratamento farmacológico
Smilax/imunologia
[Mh] Termos MeSH secundário: Animais
Células Endoteliais/metabolismo
Células Endoteliais/patologia
Glicocálix/metabolismo
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Interleucina-6/metabolismo
Lipopolissacarídeos/imunologia
Pulmão/patologia
Masculino
Camundongos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Infiltração de Neutrófilos/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Flavonols); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Tumor Necrosis Factor-alpha); 29838-67-3 (astilbin); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170805
[Lr] Data última revisão:
170805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160426
[St] Status:MEDLINE


  9 / 171 MEDLINE  
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[PMID]:27060210
[Au] Autor:Zhao BT; Le DD; Nguyen PH; Ali MY; Choi JS; Min BS; Shin HM; Rhee HI; Woo MH
[Ad] Endereço:College of Pharmacy, Catholic University of Daegu, Gyeongsan 38430, Republic of Korea.
[Ti] Título:PTP1B, α-glucosidase, and DPP-IV inhibitory effects for chromene derivatives from the leaves of Smilax china L.
[So] Source:Chem Biol Interact;253:27-37, 2016 Jun 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Two new flavonoids, bismilachinone (11) and smilachinin (14), were isolated from the leaves of Smilax china L. together with 14 known compounds. Their structures were elucidated using spectroscopic methods. The PTP1B, α-glucosidase, and DPP-IV inhibitory activities of compounds 1-16 were evaluated at the molecular level. Among them, compounds 4, 7, and 10 showed moderate DPP-IV inhibitory activities with IC50 values of 20.81, 33.12, and 32.93 µM, respectively. Compounds 3, 4, 6, 11, 12, and 16 showed strong PTP1B inhibitory activities, with respective IC50 values of 7.62, 10.80, 0.92, 2.68, 9.77, and 24.17 µM compared with the IC50 value for the positive control (ursolic acid: IC50 = 1.21 µM). Compounds 2-7, 11, 12, 15, and 16 showed potent α-glucosidase inhibitory activities, with respective IC50 values of 8.70, 81.66, 35.11, 35.92, 7.99, 26.28, 11.28, 62.68, 44.32, and 70.12 µM. The positive control, acarbose, displayed an IC50 value of 175.84 µM. In the kinetic study for the PTP1B enzyme, compounds 6, 11, and 12 displayed competitive inhibition with Ki values of 3.20, 8.56, and 5.86 µM, respectively. Compounds 3, 4, and 16 showed noncompetitive inhibition with Ki values of 18.75, 5.95, and 22.86 µM, respectively. Molecular docking study for the competitive inhibitors (6, 11, and 12) radically corroborates the binding affinities and inhibition of PTP1B enzymes. These results indicated that the leaves of Smilax china L. may contain compounds with anti-diabetic activity.
[Mh] Termos MeSH primário: Benzopiranos/química
Dipeptidil Peptidase 4/química
Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores
Smilax/química
[Mh] Termos MeSH secundário: Benzopiranos/metabolismo
Sítios de Ligação
Dipeptidil Peptidase 4/metabolismo
Cinética
Espectroscopia de Ressonância Magnética
Folhas de Planta/química
Folhas de Planta/metabolismo
Estrutura Terciária de Proteína
Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo
Smilax/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrans); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1); EC 3.4.14.5 (Dipeptidyl Peptidase 4)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160410
[St] Status:MEDLINE


  10 / 171 MEDLINE  
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[PMID]:26840656
[Au] Autor:Ki NY; Park EJ; Sung Is; Ju SA; Kim KU; Kim MR; Song DY; Lee MJ; Kim HS; Kang BH; Chung HJ; Choi EJ; Yoon KH; Lee MW; Yun S; Min B; Kwon SH; Shin HS
[Ad] Endereço:Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
[Ti] Título:The Hot-Water Extract of Smilacis Chinae Rhizome Suppresses 2,4-Dinitrochlorobenzene and House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions in Mice.
[So] Source:Phytother Res;30(4):636-45, 2016 04.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Smilacis Chinae Rhizome (SCR) has been used as an oriental folk medicine for various biological activities. However, its effect on atopic dermatitis (AD) remains undetermined to date. We assessed the effect of orally administered hot-water extract of SCR on AD-like skin lesions in mice and its underlying mechanisms. AD-like murine model was prepared by repeated alternate application of house dust mite (Dermatophagoides farinae) extract (DFE) and 2,4-dinitrochlorobenzene (DNCB) for 4 weeks, topically to the ears. Daily oral administration of SCR for 3 and 4 weeks significantly reduced inflammatory ear thickening, with the effect being enhanced at the earlier start and longer period of administration. This effect was accompanied by a significant decrease in both Th2 and Th1 serum antibodies (total IgE, DFE-specific IgE, and IgG2a). Histological analysis showed that SCR markedly decreased the epidermal/dermal ear thickening and the dermal infiltration of inflammatory cells. Furthermore, SCR suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-17, IL-18, TSLP, and IFN-γ genes in the ear tissue. Taken together, our observations demonstrate that chronic oral administration of SCR exerts beneficial effect in mouse AD model, suggesting that SCR has the therapeutic potential as an orally active treatment of AD by modulating both Th1 and Th2 responses.
[Mh] Termos MeSH primário: Dermatite Atópica/tratamento farmacológico
Extratos Vegetais/farmacologia
Pele/efeitos dos fármacos
Smilax/química
[Mh] Termos MeSH secundário: Animais
Dermatite Atópica/induzido quimicamente
Dermatophagoides farinae/imunologia
Dinitroclorobenzeno/efeitos adversos
Modelos Animais de Doenças
Feminino
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Interleucinas/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Rizoma/química
Pele/patologia
Células Th1/imunologia
Células Th2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Interleukins); 0 (Plant Extracts); 37341-29-0 (Immunoglobulin E); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5573



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