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Pesquisa : B01.650.940.800.575.912.250.618.937.124 [Categoria DeCS]
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[PMID]:28480734
[Au] Autor:Kumar A; Chand G; Agnihotri VK
[Ad] Endereço:a Academy of Scientific and Innovative Research , CSIR-Institute of Himalayan Bioresource Technology , Palampur , India.
[Ti] Título:A new oxo-sterol derivative from the rhizomes of Costus speciosus.
[So] Source:Nat Prod Res;32(1):18-22, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemical investigation of the rhizomes of Costus speciosus led to the isolation of a new compound, 22-ketocholesteryl palmitate (1) along with four known compounds, 24-methylenecycloartanol (2), cycloartanol (3), stigmasterol (4) and linoleic acid (5). The structure of new compound was characterised by extensive 1D-, 2D-NMR and mass spectrometry (GC-MS and HR-ESI-MS) techniques.
[Mh] Termos MeSH primário: Costus/química
Cetosteroides/química
Rizoma/química
Esteróis/química
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Ácido Linoleico/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Espectrometria de Massas por Ionização por Electrospray/métodos
Estigmasterol/química
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (24-methylenecycloartenol); 0 (Ketosteroids); 0 (Sterols); 0 (Triterpenes); 99WUK5D0Y8 (Stigmasterol); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1324962


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[PMID]:29310671
[Au] Autor:Mohamad S; Ismail NN; Parumasivam T; Ibrahim P; Osman H; A Wahab H
[Ad] Endereço:School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia. suri@usm.my.
[Ti] Título:Antituberculosis activity, phytochemical identification of Costus speciosus (J. Koenig) Sm., Cymbopogon citratus (DC. Ex Nees) Stapf., and Tabernaemontana coronaria (L.) Willd. and their effects on the growth kinetics and cellular integrity of Mycobacterium tuberculosis H37Rv.
[So] Source:BMC Complement Altern Med;18(1):5, 2018 Jan 08.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Costus speciosus, Cymbopogon citratus, and Tabernaemontana coronaria are herbal plants traditionally used as remedies for symptoms of tuberculosis (TB) including cough. The aims of the present study were to evaluate the in vitro anti-TB activity of different solvent partitions of these plants, to identify the phytochemical compounds, and to assess the effects of the most active partitions on the growth kinetics and cellular integrity of the tubercle organism. METHODS: The in vitro anti-TB activity of different solvent partitions of the plant materials was determined against M. tuberculosis H37Rv using a tetrazolium colorimetric microdilution assay. The phytochemical compounds in the most active partition of each plant were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The effects of these partitions on the growth kinetics of the mycobacteria were evaluated over 7-day treatment period in a batch culture system. Their effects on the mycobacterial cellular integrity were observed under a scanning electron microscope (SEM). RESULTS: The respective n-hexane partition of C. speciosus, C. citratus, and T. coronaria exhibited the highest anti-TB activity with minimum inhibitory concentrations (MICs) of 100-200 µg/mL and minimum bactericidal concentration (MBC) of 200 µg/mL. GC-MS phytochemical analysis of these active partitions revealed that majority of the identified compounds belonged to lipophilic fatty acid groups. The active partitions of C. speciosus and T. coronaria exhibited high cidal activity in relation to time, killing more than 99% of the cell population. SEM observations showed that these active plant partitions caused multiple structural changes indicating massive cellular damages. CONCLUSIONS: The n-hexane partition of the plant materials exhibited promising in vitro anti-TB activity against M. tuberculosis H37Rv. Their anti-TB activity was supported by their destructive effects on the integrity of the mycobacterial cellular structure.
[Mh] Termos MeSH primário: Antituberculosos/farmacologia
Costus/química
Mycobacterium tuberculosis/efeitos dos fármacos
Extratos Vegetais/farmacologia
Tabernaemontana/química
[Mh] Termos MeSH secundário: Antituberculosos/química
Cromatografia Gasosa-Espectrometria de Massas
Cinética
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2077-5


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[PMID]:27515456
[Au] Autor:El-Far AH; Badria FA; Shaheen HM
[Ad] Endereço:Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt.
[Ti] Título:Possible Anticancer Mechanisms of Some Costus speciosus Active Ingredients Concerning Drug Discovery.
[So] Source:Curr Drug Discov Technol;13(3):123-143, 2016.
[Is] ISSN:1875-6220
[Cp] País de publicação:United Arab Emirates
[La] Idioma:eng
[Ab] Resumo:Costus speciosus is native to South East Asia, especially found in India, Srilanka, Indonesia and Malaysia. C. speciosus have numerous therapeutic potentials against a wide variety of complains. The therapeutic properties of C. speciosus are attributed to the presence of various ingredients such as alkaloids, flavonoids, glycosides, phenols, saponins, sterols and sesquiterpenes. This review presented the past, present, and the future status of C. speciosus active ingredients to propose a future use as a potential anticancer agent. All possible up-regulation of cellular apoptotic molecules as p53, p21, p27, caspases, reactive oxygen species (ROS) generation and others attribute to the anticancer activity of C. speciosus along the down-regulation of anti-apoptotic agents such as Akt, Bcl2, NFKB, STAT3, JAK, MMPs, actin, surviving and vimentin. Eventually, we recommend further investigation of different C. speciosus extracts, using some active ingredients and evaluate the anticancer effect of these chemicals against different cancers.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Costus
Neoplasias/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Descoberta de Drogas
Seres Humanos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE


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Carvalho, Jose Carlos Tavares
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[PMID]:27306958
[Au] Autor:Picanço LC; Bittencourt JA; Henriques SV; da Silva JS; Oliveira JM; Ribeiro JR; Sanjay AB; Carvalho JC; Stien D; Silva JO
[Ad] Endereço:a Toxicology Laboratory, Pharmaceutical Science Course , Federal University of Amapá , Macapa , Brazil ;
[Ti] Título:Pharmacological activity of Costus spicatus in experimental Bothrops atrox envenomation.
[So] Source:Pharm Biol;54(10):2103-10, 2016 Oct.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Medicinal plants encompass a rich source of active compounds that can neutralize snake venoms or toxins. Costus spicatus (Jacq.) Sw. (Costaceae) is used by the Amazonian population to treat inflammation, pain and other pathological manifestations. OBJECTIVE: To evaluate the influence of C. spicatus aqueous extract on edema, peritonitis, nociception, coagulation, haemorrhage and indirect haemolytic activity induced by Bothrops atrox venom (BAV). MATERIALS AND METHODS: Dried and pulverized leaves were extracted with distilled water. Envenoming was induced by administration of B. atrox snake venom in Swiss Webster mice. The experimental groups consisted of BAV (at the minimum dose to induce measurable biological responses) and C. spicatus extract (CSE, 1.25, 2.5, 5.0, 7.5 and 10 mg/kg/25 µl phosphate-buffered saline) administered individually and in combination (BAVCSE). PBS was used as a control. In vitro assays were also conducted in order to evaluate phospholipase A2 coagulant activities (indirect haemolytic method). RESULTS: CSE significantly reduced the venom-induced edema and nociception at all concentrations tested and inhibited migration of inflammatory cells at the three least concentrations (5.0, 7.5 and 10 mg/kg/25 µl PBS). CSE was not effective in inhibiting coagulant, haemorrhagic and indirect haemolytic activities of the venom. DISCUSSION AND CONCLUSION: The data suggest that CSE could exhibit a central mechanism for pain inhibition, and may also inhibit prostaglandin synthesis. These findings corroborate the traditional administration of C. spicatus decoction to treat inflammatory disorders, including those caused by B. atrox envenomation.
[Mh] Termos MeSH primário: Antídotos/farmacologia
Bothrops
Costus
Extratos Vegetais/farmacologia
Mordeduras de Serpentes/tratamento farmacológico
Venenos de Víboras
[Mh] Termos MeSH secundário: Analgésicos/isolamento & purificação
Analgésicos/farmacologia
Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Antídotos/isolamento & purificação
Coagulação Sanguínea/efeitos dos fármacos
Costus/química
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Edema/tratamento farmacológico
Hemólise/efeitos dos fármacos
Hemorragia/sangue
Hemorragia/tratamento farmacológico
Masculino
Nociceptividade/efeitos dos fármacos
Dor Nociceptiva/tratamento farmacológico
Dor Nociceptiva/fisiopatologia
Peritonite/tratamento farmacológico
Fosfolipases A2/metabolismo
Fitoterapia
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Plantas Medicinais
Mordeduras de Serpentes/sangue
Mordeduras de Serpentes/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Antidotes); 0 (Plant Extracts); 0 (Viper Venoms); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2016.1145703


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[PMID]:26854130
[Au] Autor:Hardikar MR; Varma ME; Kulkarni AA; Kulkarni PP; Joshi BN
[Ad] Endereço:Bioprospecting Group, Agharkar Research Institute, Pune 411 004, India.
[Ti] Título:Elucidation of hypoglycemic action and toxicity studies of insulin-like protein from Costus igneus.
[So] Source:Phytochemistry;124:99-107, 2016 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have reported earlier, an orally active insulin-like protein (ILP) from Costus igneus having potent hypoglycemic property in STZ-induced diabetic Swiss mice. The blood glucose level was reduced significantly within two hours after feeding ILP orally in an oral glucose tolerance test. The present study elucidates the mechanism underlying the hypoglycemic action of ILP. Mechanism of action of ILP was studied in differentiated L6 myotubes. 2-NBDG uptake stimulated by ILP was studied in differentiated L6 myotubes under normoglycemic, hyperglycemic and induced insulin resistant conditions. ILP treatment significantly increased 2-NBDG uptake in differentiated L6 myotubes. The levels of insulin signaling molecules IRS-1 and GLUT-4 were assessed in ILP treated L6 myotubes by immunoblot analysis of cytoplasmic and plasma membrane fractions respectively. Immunoblot analysis revealed an increase in cytoplasmic IRS-1 with a concomitant increase in GLUT-4 translocation to the plasma membrane in a time dependent manner. Toxicity studies of ILP were performed on normal as well as diabetic Swiss albino mice. ILP did not show any toxicity in the acute and sub-chronic toxicity studies in normal as well as diabetic Swiss albino mice. Mass spectrometry was carried out to identify ILP. MALDI TOF/TOF MS analysis of ILP revealed sequence homology with the predicted protein from Physcomitrella patens. Our study reveals that ILP acts via insulin signaling pathway and can be used as oral insulin mimetic.
[Mh] Termos MeSH primário: Costus/química
Diabetes Mellitus Experimental/tratamento farmacológico
Hipoglicemiantes/isolamento & purificação
Hipoglicemiantes/farmacologia
Insulina/farmacologia
[Mh] Termos MeSH secundário: 4-Cloro-7-nitrobenzofurazano/análogos & derivados
4-Cloro-7-nitrobenzofurazano/farmacologia
Animais
Glicemia/metabolismo
Desoxiglucose/análogos & derivados
Desoxiglucose/farmacologia
Diabetes Mellitus Experimental/sangue
Modelos Animais de Doenças
Teste de Tolerância a Glucose
Hipoglicemiantes/química
Camundongos
Músculo Esquelético/efeitos dos fármacos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Tumor Necrosis Factor-alpha); 9G2MP84A8W (Deoxyglucose); EQF2794IRE (4-Chloro-7-nitrobenzofurazan); JE4F4P486R (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


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[PMID]:26778604
[Au] Autor:Alonso-Castro AJ; Zapata-Morales JR; González-Chávez MM; Carranza-Álvarez C; Hernández-Benavides DM; Hernández-Morales A
[Ad] Endereço:Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, México. Electronic address: angeljosabad@ugto.mx.
[Ti] Título:Pharmacological effects and toxicity of Costus pulverulentus C. Presl (Costaceae).
[So] Source:J Ethnopharmacol;180:124-30, 2016 Mar 02.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Costus pulverulentus C. Presl (Costaceae), a species endemic to Mexico, is used for the empirical treatment of cancer, pain, and inflammation. AIM OF THE STUDY: The objective of this study was to evaluate the toxicity, as well as the cytotoxic, antinociceptive, anti-inflammatory and sedative effects of an ethanol extract from Costus pulverulentus stem (CPE). MATERIALS AND METHODS: The chemical characterization of CPE was performed by Gas chromatography-mass spectrometry (GC-MS). The toxicity of CPE was evaluated using the comet assay (10-1000 µg/ml during 5h) and the acute toxicity test (500-5000 mg/kg p.o. and i.p. during 14 days). The cytotoxic effect of CPE (1-250 µg/ml) on human cancer cells was evaluated using the MTT assay. The antinociceptive effects of CPE (50-200mg/kg p.o.) were evaluated using thermal-induced nociception tests (hot plate and tail flick) and the chemical-induced nociceptive tests (acetic acid and formalin). The sedative activity of CPE (50-200mg/kg p.o.) was evaluated using the ketamine-induced sleeping time test. RESULTS: CPE showed the presence of compounds such as campesterol, stigmasterol ß-sitosterol, vanillic acid, among others. In the comet assay, CPE at 200 µg/ml or higher concentrations induced DNA damage. In the acute toxicity test, the LD50 estimated for CPE was>5000 mg/kg p.o. or i.p. CEP showed moderate cytotoxic effects on prostate carcinoma cells PC-3 cells (IC50=179 ± 23.2 µg/ml). In the chemical-induced nociception models, CPE (100 and 200mg/kg p.o.) showed antinociceptive effects with similar activity to 100mg/kg naproxen. In the thermal-induced nociception tests, CPE tested at 200mg/kg showed moderate antinociceptive effects by 28% (hot plate test) and by 25% (tail flick test). In the ketamine-induced sleeping time test, CPE showed no sedative effects. CONCLUSIONS: C. pulverulents exerts moderate cytotoxic effects in human cancer cells, moderate anti-inflammatory and antinociceptive effects. C. pulverulentus induces antinociceptive effects without inducing sedation.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
Costus
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Ácido Acético
Analgésicos/uso terapêutico
Analgésicos/toxicidade
Anestésicos Dissociativos/farmacologia
Animais
Anti-Inflamatórios/uso terapêutico
Anti-Inflamatórios/toxicidade
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Ensaio Cometa
Edema/tratamento farmacológico
Formaldeído
Temperatura Alta
Seres Humanos
Ketamina/farmacologia
Dose Letal Mediana
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Masculino
Camundongos Endogâmicos BALB C
Dor/induzido quimicamente
Dor/tratamento farmacológico
Extratos Vegetais/uso terapêutico
Extratos Vegetais/toxicidade
Caules de Planta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Analgesics); 0 (Anesthetics, Dissociative); 0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 1HG84L3525 (Formaldehyde); 690G0D6V8H (Ketamine); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160119
[St] Status:MEDLINE


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[PMID]:26727889
[Au] Autor:Perera HK; Premadasa WK; Poongunran J
[Ad] Endereço:Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. kumudup@pdn.ac.lk.
[Ti] Título:α-glucosidase and glycation inhibitory effects of costus speciosus leaves.
[So] Source:BMC Complement Altern Med;16:2, 2016 Jan 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hyperglycaemia is a salient feature of poorly controlled diabetes mellitus. Rate of protein glycation is increased with hyperglycaemia leading to long term complications of diabetes. One approach of controlling blood glucose in diabetes targets at reducing the postprandial spikes of blood glucose. The objectives of this study were to assess the in vitro inhibitory effects of Costus speciosus (COS) leaves on α-amylase and α-glucosidase activities, fructosamine formation, protein glycation and glycation-induced protein cross-linking. METHODS: Methanol extracts of COS leaves were used. Inhibitory effects on enzyme activities were measured using porcine pancreatic α-amylase and α-glucosidase from Saccharomyces cerevisiae in the presence of COS extract. Percentage inhibition of the enzymes and the IC50 values were determined. In vitro protein glycation inhibitory effect of COS leaves on early and late glycation products were measured using bovine serum albumin or chicken egg lysozyme with fructose. Nitroblue tetrazolium was used to assess the relative concentration of fructosamine and polyacrylamide gel electrophoresis was used to assess the degree of glycation and protein cross-linking in the reaction mixtures. RESULTS: α-Glucosidase inhibitory activity was detected in COS leaves with a IC50 of 67.5 µg/ml which was significantly lower than the IC50 value of Acarbose (p < 0.01). Amylase inhibitory effects occurred at a comparatively higher concentration of extract with a IC50 of 5.88 mg/ml which was significantly higher than the IC50 value of Acarbose (p < 0.01). COS (250 µg/ml) demonstrated inhibitory effects on fructosamine formation and glycation induced protein cross-linking which were in par with 1 mg/ml aminoguanidine were detected. CONCLUSION: Methanol extracts of COS leaves demonstrated in vitro inhibitory activities on α-glucosidase, fructosamine formation, glycation and glycation induced protein cross-linking. These findings provide scientific evidence to support the use of COS leaves for hypoglycemic effects with an added advantage in slowing down protein glycation.
[Mh] Termos MeSH primário: Costus/química
Inibidores de Glicosídeo Hidrolases/farmacologia
Folhas de Planta
alfa-Amilases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Frutosamina/biossíntese
Produtos Finais de Glicação Avançada/metabolismo
Glicosilação/efeitos dos fármacos
alfa-Glucosidases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycation End Products, Advanced); 0 (Glycoside Hydrolase Inhibitors); 4429-04-3 (Fructosamine); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160106
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-015-0982-z


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[PMID]:26490377
[Au] Autor:Das A; Das P; Kalita MC; Mondal TK
[Ad] Endereço:Department of Bioengineering and Technology, Gauhati University-Institute of Science and Technology, Gopinath Bordoloi Nagar, Guwahati, Assam, 781014, India. dasakan@gmail.com.
[Ti] Título:Computational Identification, Target Prediction, and Validation of Conserved miRNAs in Insulin Plant (Costus pictus D. Don).
[So] Source:Appl Biochem Biotechnol;178(3):513-26, 2016 Feb.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insulin plant (Costus pictus D. Don) is an economically important medicinal plant for the content of its high value secondary metabolites, bioactive compounds, and remarkable flowering features. MicroRNAs are a class of short (∼21 nucleotides), endogenous, noncoding RNA molecules that play a vital role in regulating gene expression. Here, we used a computer-based homology approach to identify conserved miRNAs in Transcribed Sequence Assemblies (TSA) of C. pictus. It led us to identify 42 miRNAs of 13 different families in C. pictus for the first time. Using quantitative polymerase chain reaction (qPCR) assays, we further confirmed the expression of 8 miRNAs (miR394, miR159b, miR166k, miR172, miR159f, miR166, miR144, and miR858) in young and mature leaf tissues. A total of 109 potential target genes of the identified miRNAs were subsequently predicted in rice (Oryza sativa L.) genome. The target genes encode transcription factors, enzymes, and various functional proteins involved in the regulation of several metabolic pathways. The findings in the present study lay the foundation for further research on miRNAs and miRNA-mediated gene regulation in this important medicinal plant.
[Mh] Termos MeSH primário: Costus/genética
MicroRNAs/genética
[Mh] Termos MeSH secundário: Expressão Gênica
Folhas de Planta/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-015-1891-9


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[PMID]:26222585
[Au] Autor:Selim S; Al Jaouni S
[Ad] Endereço:a Department of Clinical Laboratory Sciences , College of Applied Medical Sciences, Aljouf University , Sakaka , Saudi Arabia.
[Ti] Título:Anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm.
[So] Source:Nat Prod Res;30(16):1830-3, 2016 Aug.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Costus speciosus is an important medicinal plant widely used in several indigenous medicinal formulations. The present study was conducted to evaluate the in vitro anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from C. speciosus. The diosgenin was isolated from C. speciosus by HPTLC and its biological activities were studied by different protocols. The results demonstrated that LPS stimulated TNF-α generation in RAW 264.7 macrophage culture supernatant up to 3.7-fold of the control and that sample treatment (50 µg/mL) resulted in a highly significant inhibitory effect on LPS-stimulated TNF-α (p < 0.01) in a similar manner to methotrexate inhibitory effect. The tested sample possessed an effective antioxidant scavenging affinity against DPPH radicals as compared with the standard antioxidant activity of vitamin C. The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer, apoptotic and inhibitory effects on cell proliferation.
[Mh] Termos MeSH primário: Costus/química
Diosgenina/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Inibidores da Angiogênese/isolamento & purificação
Inibidores da Angiogênese/farmacologia
Animais
Anti-Inflamatórios/farmacologia
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Apoptose/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Diosgenina/isolamento & purificação
Lipopolissacarídeos
Camundongos
Extratos Vegetais/farmacologia
Células RAW 264.7
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha); K49P2K8WLX (Diosgenin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150730
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1065493


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[PMID]:26593213
[Au] Autor:Al-Attas AA; El-Shaer NS; Mohamed GA; Ibrahim SR; Esmat A
[Ad] Endereço:Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
[Ti] Título:Anti-inflammatory sesquiterpenes from Costus speciosus rhizomes.
[So] Source:J Ethnopharmacol;176:365-74, 2015 Dec 24.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Costus speciosus (Koen ex. Retz.) Sm. (crepe ginger, family Costaceae) is an ornamental plant used in traditional medicine for the treatment of inflammation, rheumatism, bronchitis, fever, headache, asthma, flatulence, constipation, helminthiasis, leprosy, skin diseases, hiccough, anemia, as well as burning sensation on urination. AIM OF THE STUDY: The present study is designed to isolate and identify the active compounds from C. speciosus rhizomes and measure their anti-inflammatory activities. MATERIALS AND METHODS: The n-hexane-CHCl3 soluble fraction of the MeOH extract of C. speciosus rhizomes has been subjected to a repeated column chromatography, including normal silica gel and RP-18 column to give eight compounds. The structures of these compounds were established by UV, IR, 1D ((1)H and (13)C), and 2D ((1)H-(1)H COSY, NOESY, HSQC, and HMBC) NMR experiments and HRESIMS data. In addition, the anti-inflammatory activity of compounds 1-8 was evaluated by measuring the levels IL-6, IL-1ß, TNF-α, COX-2, lipoxgenase-5, and PGE2 using enzyme-linked immunosorbent assay. RESULTS: The n-hexane-CHCl3 soluble fraction afforded a new eudesmane acid, specioic acid (8), along with seven known compounds, 22,23-dihydrospinasterone (1), dehydrodihydrocostus lactone (mokko lactone) (2), dehydrocostus lactone (3), stigmasterol (4), arbusculin A (5), santamarine (douglanin) (6), and reynosin (7). Compounds 1, 4, and 5-7 were isolated for the first time C. speciosus. Compounds 1-4 displayed potent anti-inflammatory activity, while 7 and 8 showed moderate activity. Compounds 1-8 exhibited a concentration-related decrease in the levels of IL-1ß, IL-6, TNF-α, PGE2, lipoxgenase-5, and COX-2. Compounds 5 and 6 did not significantly decrease levels of different cytokines, PGE2, lipoxgenase-5, and COX-2 from PHA treatment at 1 µM. However, all tested compounds significantly decreased cytokines, PGE2, lipoxgenase-5, and COX-2 levels at concentration 100 µM. It is noteworthy that compounds 1-4 had the highest activity, where it lowered levels of cytokines, PGE2, lipoxgenase-5, and COX-2 to the extent that was no statistical difference from the control group. Thus, they decreased proinflammatory cytokines (IL-1ß, IL-6, and TNF-α) with decreased level of the target enzymes (COX-2 and lipoxgenase-5) and subsequent reduction of its inflammatory product (PGE2). CONCLUSION: Good anti-inflammatory activities exhibited of the isolated compounds from C. speciosus corroborate the usefulness of this plant in the traditional treatment of inflammation and related symptoms.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Costus
Sesquiterpenos/farmacologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/isolamento & purificação
Células Cultivadas
Ciclo-Oxigenase 2/metabolismo
Citocinas/metabolismo
Dinoprostona/metabolismo
Seres Humanos
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Lipoxigenases/metabolismo
Extratos Vegetais/química
Rizoma
Sesquiterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Plant Extracts); 0 (Sesquiterpenes); EC 1.13.11.- (Lipoxygenases); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151124
[St] Status:MEDLINE



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