Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.618.937.900.444 [Categoria DeCS]
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  1 / 1204 MEDLINE  
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[PMID]:28554232
[Au] Autor:Guo T; Tan SB; Wang Y; Chang J
[Ad] Endereço:a School of Life Science and Engineering , Lanzhou University of Technology , Lanzhou , China.
[Ti] Título:Two new monoterpenoid glycosides from the fresh rhizome of Tongling White Ginger (Zingiber officinale).
[So] Source:Nat Prod Res;32(1):71-76, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new monoterpenoid glycosides, trans-1,8-cineole-3,6-dihydroxy-3-O-ß-D-glucopyranoside (1), and 5,9-dihydroxy borneol 2-O-ß-D-glucopyranoside (2), together with four known monoterpenoid glycosides (3-6), were isolated from the water-soluble constituents of the fresh rhizome of Tongling White Ginger (Zingiber officinale). Their structures were decisively elucidated by spectroscopic analysis. In vitro tests for antimicrobial activity showed that compounds 1 and 3 possess significant activity against two Gram-positive organisms, Staphylococcus aureus and Staphylococcus epidermidis.
[Mh] Termos MeSH primário: Antibacterianos/química
Gengibre/química
Glicosídeos/química
Monoterpenos/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Glicosídeos/farmacologia
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Estrutura Molecular
Monoterpenos/farmacologia
Rizoma/química
Solubilidade
Espectrometria de Massas por Ionização por Electrospray
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Glycosides); 0 (Monoterpenes)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1333994


  2 / 1204 MEDLINE  
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[PMID]:28745358
[Au] Autor:Zhang F; Thakur K; Hu F; Zhang JG; Wei ZJ
[Ad] Endereço:School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China. china_zhangfang@163.com kumarikiran@hfut.edu.cn hufei@hfut.edu.cn zhangjianguo@hfut.edu.cn zjwei@hfut.edu.cn.
[Ti] Título:Cross-talk between 10-gingerol and its anti-cancerous potential: a recent update.
[So] Source:Food Funct;8(8):2635-2649, 2017 Aug 01.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Since time immortal, ginger, as an ancient herb, has been used throughout the world in foods and beverages due to its typical strong and pungent flavor. Besides its use as a spice, it also serves as an excellent source of several bioactive phenolics, including nonvolatile pungent compounds, such as gingerols, paradols, shogaols, and gingerones. Gingerols constitute key ingredients in fresh ginger, with the most abundant being 6-gingerol (6-G), 8-gingerol (8-G), and 10-gingerol (10-G). Many studies have investigated the various valuable pharmacological properties of these ingredients and experimentally verified the mechanistic aspects of their health effects; however, to date, most research on the anti-cancerous activities of gingerols have focused largely on 6-G. Thus, the present article deals with the number of recent studies that have indicated and highlighted the role of 10-G with respect to its cancer prevention attributes in particular and its anti-inflammatory, anti-oxidant, anti-microbial, and gastrointestinal tract protective potential in general. The purpose of this review is to provide an overview of all the experimentally validated health benefits of 10-G for nutraceutical applications. The various findings have warranted the further investigation of 10-G and its possible use in various cancer treatments as well as its promising role as a chemo-preventive agent.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Catecóis/farmacologia
Álcoois Graxos/farmacologia
Gengibre/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Catecóis/química
Suplementos Nutricionais/análise
Álcoois Graxos/química
Seres Humanos
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Catechols); 0 (Fatty Alcohols); 0 (Plant Extracts); 925QK2Z900 (gingerol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00844a


  3 / 1204 MEDLINE  
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[PMID]:28873658
[Au] Autor:Wu CY; Kong M; Zhang W; Long F; Zhou J; Zhou SS; Xu JD; Xu J; Li SL
[Ad] Endereço:Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, People's Republic of China.
[Ti] Título:Impact of sulphur fumigation on the chemistry of ginger.
[So] Source:Food Chem;239:953-963, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ginger (Zingiberis Rhizoma), a commonly-consumed food supplement, is often sulphur-fumigated during post-harvest handling, but it remains unknown if sulphur fumigation induces chemical transformations in ginger. In this study, the effects of sulphur fumigation on ginger chemicals were investigated by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS)-based metabolomics. The results showed that sulphur fumigation significantly altered the holistic chemical profile of ginger by triggering chemical transformations of certain original components. 6-Gingesulphonic acid, previously reported as a naturally-occurring component in ginger, was revealed to be a sulphur fumigation-induced artificial derivative, which was deduced to be generated by electrophilic addition of 6-shogaol to sulphurous acid. Using UHPLC-QTOF-MS/MS extracting ion analysis with 6-gingesulphonic acid as a characteristic chemical marker, all the commercial ginger samples inspected were determined to be sulphur-fumigated. The research outcomes provide a chemical basis for further comprehensive safety and efficacy evaluations of sulphur-fumigated ginger.
[Mh] Termos MeSH primário: Gengibre
[Mh] Termos MeSH secundário: Catecóis
Cromatografia Líquida de Alta Pressão
Fumigação
Enxofre
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 70FD1KFU70 (Sulfur); 83DNB5FIRF (shogaol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  4 / 1204 MEDLINE  
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[PMID]:28873530
[Au] Autor:Si W; Chen YP; Zhang J; Chen ZY; Chung HY
[Ad] Endereço:Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China; Suzhou Polytechnic Institute of Agriculture, Suzhou, Jiangsu, China. Electronic address: swh8614@163.com.
[Ti] Título:Antioxidant activities of ginger extract and its constituents toward lipids.
[So] Source:Food Chem;239:1117-1125, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lipid oxidation-a major cause of food product deterioration-necessitates the use of food additives to inhibit food oxidation. Ginger extract (GE) has been reported to possess antioxidant properties. However, components isolated from ginger have been rarely reported to inhibit fat oxidation. Herein, antioxidant properties of GE and four pure components derived from it (6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol) were examined and their properties were compared to those of butylated hydroxytoluene. GE and the constituent components exhibited antioxidant properties that might be attributed to their hydroxyl groups and suitable solubilizing side chains. 6-Shogaol and 10-gingerol exhibited higher activity at 60°C than 6-gingerol and 8-gingerol. Low antioxidant activity was detected at high temperatures (120/180°C). Overall, GE displayed the strongest dose-dependent antioxidant properties, especially at high temperatures, thereby demonstrating that GE can be employed as a natural antioxidant in lipid-containing processed foods.
[Mh] Termos MeSH primário: Gengibre
[Mh] Termos MeSH secundário: Catecóis
Álcoois Graxos
Lipídeos
Extratos Vegetais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 0 (Fatty Alcohols); 0 (Lipids); 0 (Plant Extracts)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  5 / 1204 MEDLINE  
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[PMID]:28793909
[Au] Autor:Samad MB; Mohsin MNAB; Razu BA; Hossain MT; Mahzabeen S; Unnoor N; Muna IA; Akhter F; Kabir AU; Hannan JMA
[Ad] Endereço:Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA. mehdi.samad@unmc.edu.
[Ti] Título:[6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic ß-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Lepr type 2 diabetic mice.
[So] Source:BMC Complement Altern Med;17(1):395, 2017 Aug 09.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: [6]-Gingerol, a major component of Zingiber officinale, was previously reported to ameliorate hyperglycemia in type 2 diabetic mice. Endocrine signaling is involved in insulin secretion and is perturbed in db/db Type-2 diabetic mice. [6]-Gingerol was reported to restore the disrupted endocrine signaling in rodents. In this current study on Lepr diabetic mice, we investigated the involvement of endocrine pathway in the insulin secretagogue activity of [6]-Gingerol and the mechanism(s) through which [6]-Gingerol ameliorates hyperglycemia. METHODS: Lepr type 2 diabetic mice were orally administered a daily dose of [6]-Gingerol (200 mg/kg) for 28 days. We measured the plasma levels of different endocrine hormones in fasting and fed conditions. GLP-1 levels were modulated using pharmacological approaches, and cAMP/PKA pathway for insulin secretion was assessed by qRT-PCR and ELISA in isolated pancreatic islets. Total skeletal muscle and its membrane fractions were used to measure glycogen synthase 1 level and Glut4 expression and protein levels. RESULTS: 4-weeks treatment of [6]-Gingerol dramatically increased glucose-stimulated insulin secretion and improved glucose tolerance. Plasma GLP-1 was found to be significantly elevated in the treated mice. Pharmacological intervention of GLP-1 levels regulated the effect of [6]-Gingerol on insulin secretion. Mechanistically, [6]-Gingerol treatment upregulated and activated cAMP, PKA, and CREB in the pancreatic islets, which are critical components of GLP-1-mediated insulin secretion pathway. [6]-Gingerol upregulated both Rab27a GTPase and its effector protein Slp4-a expression in isolated islets, which regulates the exocytosis of insulin-containing dense-core granules. [6]-Gingerol treatment improved skeletal glycogen storage by increased glycogen synthase 1 activity. Additionally, GLUT4 transporters were highly abundant in the membrane of the skeletal myocytes, which could be explained by the increased expression of Rab8 and Rab10 GTPases that are responsible for GLUT4 vesicle fusion to the membrane. CONCLUSIONS: Collectively, our study reports that GLP-1 mediates the insulinotropic activity of [6]-Gingerol, and [6]-Gingerol treatment facilitates glucose disposal in skeletal muscles through increased activity of glycogen synthase 1 and enhanced cell surface presentation of GLUT4 transporters.
[Mh] Termos MeSH primário: Catecóis/uso terapêutico
Álcoois Graxos/uso terapêutico
Gengibre/química
Transportador de Glucose Tipo 4/metabolismo
Hiperglicemia/tratamento farmacológico
Células Secretoras de Insulina/efeitos dos fármacos
Insulina/secreção
Músculo Esquelético/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Glicemia/metabolismo
Catecóis/farmacologia
Diabetes Mellitus Tipo 2/sangue
Diabetes Mellitus Tipo 2/tratamento farmacológico
Álcoois Graxos/farmacologia
Peptídeo 1 Semelhante ao Glucagon/sangue
Glicogênio/metabolismo
Glicogênio Sintase/metabolismo
Hiperglicemia/etiologia
Hiperglicemia/metabolismo
Células Secretoras de Insulina/metabolismo
Proteínas de Membrana/metabolismo
Camundongos
Camundongos Endogâmicos NOD
Camundongos Knockout
Músculo Esquelético/metabolismo
Fitoterapia
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Via Secretória/efeitos dos fármacos
Proteínas de Transporte Vesicular/metabolismo
Proteínas rab de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Catechols); 0 (Fatty Alcohols); 0 (Glucose Transporter Type 4); 0 (Insulin); 0 (Membrane Proteins); 0 (Plant Extracts); 0 (Rab8 protein, mouse); 0 (Vesicular Transport Proteins); 89750-14-1 (Glucagon-Like Peptide 1); 9005-79-2 (Glycogen); 925QK2Z900 (gingerol); EC 2.4.1.11 (Glycogen Synthase); EC 3.6.1.- (Rab10 protein, mouse); EC 3.6.5.2 (rab GTP-Binding Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1903-0


  6 / 1204 MEDLINE  
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[PMID]:28789800
[Au] Autor:Peng LQ; Cao J; Du LJ; Zhang QD; Xu JJ; Chen YB; Shi YT; Li RR
[Ad] Endereço:College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
[Ti] Título:Rapid ultrasonic and microwave-assisted micellar extraction of zingiberone, shogaol and gingerols from gingers using biosurfactants.
[So] Source:J Chromatogr A;1515:37-44, 2017 Sep 15.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Two kinds of extraction methods ultrasonic-assisted micellar extraction (UAME) and microwave-assisted micellar extraction (MAME) coupled with ultra-high performance liquid chromatography with ultraviolet detector (UHPLC-UV) were developed and evaluated for extraction and determination of zingerone, 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Rhizoma Zingiberis and Rhizoma Zingiberis Preparata. A biosurfactant, hyodeoxycholic acid sodium salt, was used in micellar extraction. Several experimental parameters were studied separately by a univariate method. The result indicated that the MAME was more efficient than UAME. The optimal conditions of MAME were as follows: 100mM of hyodeoxycholic acid sodium salt was used as surfactant, the irradiation time was set at 10s and the extraction temperature was set at 60°C. The validation results indicated that the limits of detection were in the range of 3.80-8.11ng/mL. The average recoveries were in the range of 87.32-103.12% for the two samples at two spiking levels. Compared with other reported methods, the proposed MAME-UHPLC-UV method was more effective, quicker (10s) and more eco-friendly.
[Mh] Termos MeSH primário: Catecóis/isolamento & purificação
Técnicas de Química Analítica/métodos
Cromatografia Líquida de Alta Pressão
Álcoois Graxos/isolamento & purificação
Gengibre/química
[Mh] Termos MeSH secundário: Limite de Detecção
Micelas
Micro-Ondas
Rizoma/química
Tensoativos/química
Temperatura Ambiente
Ultrassom
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 0 (Fatty Alcohols); 0 (Micelles); 0 (Surface-Active Agents); 83DNB5FIRF (shogaol); 925QK2Z900 (gingerol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE


  7 / 1204 MEDLINE  
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[PMID]:28771369
[Au] Autor:Xiao L; Jia HF; Jeong IH; Ahn YJ; Zhu YZ
[Ad] Endereço:College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Changcheng Road, Chengyang District, Qingdao, Shandong 266-109, China.
[Ti] Título:Isolation and Characterization of 2,4-D Butyl Ester Degrading Acinetobacter sp. ZX02 from a Chinese Ginger Cultivated Soil.
[So] Source:J Agric Food Chem;65(34):7345-7351, 2017 Aug 30.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strain ZX02 was isolated from Chinese ginger cultivated soil contaminated with various pesticides, which could utilize 2,4-dichlorophenoxyacetic acid butyl ester (2,4-D butyl ester) as the sole carbon source. On the basis of the sequence analysis of 16S rRNA gene as well as the morphological, biochemical, and physiological characteristics of strain ZX02, the organism belonged to Gram-negative bacterium and was identified as Acinetobacter sp. ZX02. The strain ZX02 showed a remarkable performance in 2,4-D butyl ester degradation (100% removal in <96 h) in pure culture. Strain ZX02 was sensitive to tetracycline and resistant to amoxicillin and chloramphenicol in an antibiotic sensitivity test. The curing study indicates that the gene for degradation of 2,4-D butyl ester was encoded on a single plasmid of 23 kb. The gene encoding resistance to polymixin B sulfate was also located on this plasmid. On the basis of its greater biodegradation activity, this bacterium is a potential candidate as a bioremediation agent in soils contaminated with 2,4-D butyl ester.
[Mh] Termos MeSH primário: Acinetobacter/isolamento & purificação
Acinetobacter/metabolismo
Praguicidas/metabolismo
Microbiologia do Solo
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético
Acinetobacter/classificação
Acinetobacter/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biodegradação Ambiental
Gengibre/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Pesticides); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02140


  8 / 1204 MEDLINE  
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[PMID]:28675098
[Au] Autor:Taga Y; Hayashida O; Kusubata M; Ogawa-Goto K; Hattori S
[Ad] Endereço:a Nippi Research Institute of Biomatrix , Toride , Japan.
[Ti] Título:Production of a novel wheat gluten hydrolysate containing dipeptidyl peptidase-IV inhibitory tripeptides using ginger protease.
[So] Source:Biosci Biotechnol Biochem;81(9):1823-1828, 2017 Sep.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Wheat gluten is a Pro-rich protein complex comprising glutenins and gliadins. Previous studies have reported that oral intake of enzymatic hydrolysates of gluten has beneficial effects, such as suppression of muscle injury and improvement of hepatitis. Here, we utilized ginger protease that preferentially cleaves peptide bonds with Pro at the P position to produce a novel type of wheat gluten hydrolysate. Ginger protease efficiently hydrolyzed gluten, particularly under weak acidic conditions, to peptides with an average molecular weight of <600 Da. In addition, the gluten hydrolysate contained substantial amounts of tripeptides, including Gln-Pro-Gln, Gln-Pro-Gly, Gln-Pro-Phe, Leu-Pro-Gln, and Ser-Pro-Gln (e.g. 40.7 mg/g at pH 5.2). These gluten-derived tripeptides showed high inhibitory activity on dipeptidyl peptidase-IV with IC values of 79.8, 70.9, 71.7, 56.7, and 78.9 µM, respectively, suggesting that the novel gluten hydrolysate prepared using ginger protease can be used as a functional food for patients with type 2 diabetes.
[Mh] Termos MeSH primário: Dipeptidil Peptidase 4/metabolismo
Inibidores da Dipeptidil Peptidase IV/metabolismo
Gengibre/enzimologia
Glutens/metabolismo
Oligopeptídeos/metabolismo
Peptídeo Hidrolases/metabolismo
Triticum/química
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Hidrólise
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dipeptidyl-Peptidase IV Inhibitors); 0 (Oligopeptides); 8002-80-0 (Glutens); EC 3.4.- (Peptide Hydrolases); EC 3.4.14.5 (Dipeptidyl Peptidase 4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1345615


  9 / 1204 MEDLINE  
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[PMID]:28653916
[Au] Autor:Awan UA; Ali S; Shahnawaz AM; Shafique I; Zafar A; Khan MAR; Ghous T; Saleem A; Andleeb S
[Ad] Endereço:Biotechnology laboratory, Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
[Ti] Título:Biological activities of Allium sativum and Zingiber officinale extracts on clinically important bacterial pathogens, their phytochemical and FT-IR spectroscopic analysis.
[So] Source:Pak J Pharm Sci;30(3):729-745, 2017 May.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:The spread of bacterial infectious diseases is a major public threat. Herbs and spices have offered an excellent, important and useful source of antimicrobial agents against many pathological infections. In the current study, the antimicrobial potency of fresh, naturally and commercial dried Allium sativum and Zingiber officinale extracts had been investigated against seven local clinical bacterial isolates such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus pyogenes, Staphylococcus epidermidis, and Serratia marcesnces by the agar disc diffusion method. All tested pathogens except P. aeruginosa and E. coli were most susceptible to ethanolic and methanolic extracts of A. sativum. Similarly, chloroform and diethyl ether extracts of Z. officinale showed a greater zone of inhibition of tested pathogens except for P. aeruginosa and E. coli. We found that all extracts of A. sativum and Z. officinale have a strong antibacterial effect compared to recommended standard antibiotics through activity index. All results were evaluated statistically and a significant difference was recorded at P< 0.05. Antioxidant activity of extracts showed that 10 out of 13 extracts have high scavenging potential. Thin layer chromatography profiling of all extracts of A. sativum and Z. officinale proposed the presence of various phytochemicals such as tannins, phenols, alkaloids, steroids and saponins. Retention factor of diverse phytochemicals provides a valuable clue regarding their polarity and the selection of solvents for separation of phytochemicals. Significant inhibition of S. aureus was also observed through TLC-Bioautography. FT-IR Spectrometry was also performed to characterize both natural and commercial extracts of A. sativum and Z. officinale to evaluate bioactive compounds. These findings provide new insights to use A. sativum and Z. officinale as potential plant sources for controlling pathogenic bacteria and potentially considered as cost-effective in the management of diseases and to the threat of drug resistance phenomenon.
[Mh] Termos MeSH primário: Allium/química
Antibacterianos/farmacologia
Gengibre/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Cromatografia em Camada Delgada
Testes de Sensibilidade Microbiana
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE


  10 / 1204 MEDLINE  
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[PMID]:28646880
[Au] Autor:Seow SLS; Hong SL; Lee GS; Malek SNA; Sabaratnam V
[Ad] Endereço:Mushroom Research Centre, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
[Ti] Título:6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells.
[So] Source:BMC Complement Altern Med;17(1):334, 2017 Jun 24.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ginger is a popular spice and food preservative. The rhizomes of the common ginger have been used as traditional medicine to treat various ailments. 6-Shogaol, a pungent compound isolated from the rhizomes of jahe gajah (Zingiber officinale var officinale) has shown numerous pharmacological activities, including neuroprotective and anti-neuroinflammatory activities. The aim of this study was to investigate the potential of 6-shogaol to mimic the neuritogenic activity of nerve growth factor (NGF) in rat pheochromocytoma (PC-12) cells. METHODS: The cytotoxic effect of 6-shogaol was determined by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The neuritogenic activity was assessed by neurite outgrowth stimulation assay while the concentration of extracellular NGF in cell culture supernatant was assessed by enzyme-linked immunosorbent assay (ELISA). Involvement of cellular signaling pathways, mitogen-activated protein kinase kinase/extracellular signal-regulated kinase1/2 (MEK/ERK1/2) and phosphoinositide-3-kinase/protein kinase B (PI3K/AKT) in 6-shogaol-stimulated neuritogenesis were examined by using specific pharmacological inhibitors. RESULTS: 6-Shogaol (500 ng/ml) induced neuritogenesis that was comparable to NGF (50 ng/ml) and was not cytotoxic towards PC-12 cells. 6-Shogaol induced low level of NGF biosynthesis in PC-12 cells, showing that 6-shogaol stimulated neuritogenesis possibly by inducing NGF biosynthesis, and also acting as a substitute for NGF (NGF mimic) in PC-12 cells. The inhibitors of Trk receptor (K252a), MEK/ERK1/2 (U0126 and PD98059) and PI3K/AKT (LY294002) attenuated the neuritogenic activity of both NGF and 6-shogaol, respectively. CONCLUSIONS: The present findings demonstrated that 6-shogaol induced neuritogenic activity in PC-12 cells via the activation MEK/ERK1/2 and PI3K/AKT signaling pathways. This study suggests that 6-shogaol could act as an NGF mimic, which may be beneficial for preventive and therapeutic uses in neurodegenerative diseases.
[Mh] Termos MeSH primário: Catecóis/farmacologia
Gengibre/química
Fator de Crescimento Neural/metabolismo
Neuritos/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Catecóis/química
Catecóis/isolamento & purificação
Neuritos/metabolismo
Células PC12
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Ratos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 0 (Plant Extracts); 83DNB5FIRF (shogaol); 9061-61-4 (Nerve Growth Factor)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1837-6



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