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Pesquisa : B01.650.940.800.575.912.250.665.249 [Categoria DeCS]
Referências encontradas : 23 [refinar]
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  1 / 23 MEDLINE  
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[PMID]:27184430
[Au] Autor:Kirtz M; Klebensberger J; Otte KB; Richter SM; Hauer B
[Ad] Endereço:Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
[Ti] Título:Production of ω-hydroxy octanoic acid with Escherichia coli.
[So] Source:J Biotechnol;230:30-3, 2016 Jul 20.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like precursors. For this, we used the model organism Escherichia coli and increased primarily the release of the octanoic acid precursors by overexpressing the plant thioesterase FatB2 from Cuphea hookeriana in a ß-oxidation deficient strain, which lead to the production of 2.32mM (8.38mggcww(-1)) octanoic acid in 24h. In order to produce the corresponding ω-hydroxy derivative, we additionally expressed the engineered self-sufficient monooxygenase fusion protein CYP153AMaq(G307A)-CPRBM3 within the octanoic acid producing strain. With this, we finally produced 234µM (0.95mggcww(-1)) ω-hydroxy octanoic acid in a 20h fed-batch set-up.
[Mh] Termos MeSH primário: Caprilatos/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
[Mh] Termos MeSH secundário: Reatores Biológicos/microbiologia
Caprilatos/análise
Cuphea/enzimologia
Cuphea/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Tioléster Hidrolases/genética
Tioléster Hidrolases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caprylates); 0 (Plant Proteins); EC 3.1.2.- (Thiolester Hydrolases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE


  2 / 23 MEDLINE  
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[PMID]:27125593
[Au] Autor:Barboza LN; Lívero FA; Prando TB; Ribeiro Rde C; Lourenço EL; Budel JM; de Souza LM; Acco A; Dalsenter PR; Gasparotto A
[Ad] Endereço:Departamento de Farmacologia, Universidade Federal do Paraná, Curitiba, PR, Brazil.
[Ti] Título:Atheroprotective effects of Cuphea carthagenensis (Jacq.) J. F. Macbr. in New Zealand rabbits fed with cholesterol-rich diet.
[So] Source:J Ethnopharmacol;187:134-45, 2016 Jul 01.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Although Cuphea carthagenensis (Jacq.) J. F. Macbr. is used in Brazilian folk medicine in the treatment of atherosclerosis and circulatory disorders, no study evaluating these effects has been conducted. The aim of this study was to evaluate the possible hypolipemiant and antiatherogenic activity of the ethanol soluble fraction obtained from C. carthagenensis (ES-CC) in an experimental atherosclerosis model using New Zealand (NZ) rabbits undergoing cholesterol-rich diet (CRD). MATERIAL AND METHODS: Dyslipidemia and atherogenesis were induced by administration of standard commercial diet increased of 1% cholesterol (CRD) for 8 weeks. ES-CC was orally administered at doses of 10, 30 and 100mg/kg, once daily for four weeks, starting from the 4th week of CRD diet. Body weight measurements were weekly carried out from the beginning of experiments for 8 weeks. Serum levels of triglyceride (TG), total cholesterol (TC) and their fractions (LDL-C, VLDL-C and HDL-C) were measured at the beginning of experiments and at weeks four and eight. After euthanasia of rabbits, aorta segments (aortic arc, thoracic, abdominal and iliac segments) were macroscopically and microscopically evaluated and the intima and media layers of the arteries were measured. Additionally, the antioxidant activity of ES-CC and its influence on the functioning of hepatic antioxidant enzymes were also determined. RESULTS: CRD induced dyslipidemia and major structural changes in the aortic wall. In addition, an increase in lipid peroxidation and a reduction of hepatic glutathione and serum nitrite levels were observed. Treatment with ES-CC was able to prevent the increase in TC, LDL-C, VLDL-C levels and triglycerides and promoted an increase in HDL-C levels in NZ rabbits. These effects were accompanied by a significant reduction in oxidative stress and modulation of the catalase and superoxide dismutase function. Moreover, the intima and media layers of the arterial segments were significantly reduced by ES-CC treatment. CONCLUSIONS: This study demonstrated that ES-CC reduces serum lipids and hepatic oxidative stress when orally administered to NZ rabbits. In addition, it was able to prevent the development of CRD-induced atherosclerosis.
[Mh] Termos MeSH primário: Aterosclerose/tratamento farmacológico
Cardiotônicos/uso terapêutico
Cuphea
Hipolipemiantes/uso terapêutico
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Artérias/efeitos dos fármacos
Artérias/patologia
Aterosclerose/patologia
Cardiotônicos/farmacologia
Catalase/metabolismo
Colesterol na Dieta
Dieta Hiperlipídica
Glutationa/metabolismo
Glutationa Transferase/metabolismo
Hipolipemiantes/farmacologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Nitratos/sangue
Nitritos/sangue
Extratos Vegetais/farmacologia
Folhas de Planta
Coelhos
Superóxido Dismutase/metabolismo
Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cardiotonic Agents); 0 (Cholesterol, Dietary); 0 (Hypolipidemic Agents); 0 (Nitrates); 0 (Nitrites); 0 (Plant Extracts); 0 (Thiobarbituric Acid Reactive Substances); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160430
[St] Status:MEDLINE


  3 / 23 MEDLINE  
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[PMID]:26518537
[Au] Autor:Stefan A; Hochkoeppler A; Ugolini L; Lazzeri L; Conte E
[Ad] Endereço:Dept. of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
[Ti] Título:The expression of the Cuphea palustris thioesterase CpFatB2 in Yarrowia lipolytica triggers oleic acid accumulation.
[So] Source:Biotechnol Prog;32(1):26-35, 2016 Jan-Feb.
[Is] ISSN:1520-6033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The conversion of industrial by-products into high-value added compounds is a challenging issue. Crude glycerol, a by-product of the biodiesel production chain, could represent an alternative carbon source for the cultivation of oleaginous yeasts. Here, we developed five minimal synthetic glycerol-based media, with different C/N ratios, and we analyzed the production of biomass and fatty acids by Yarrowia lipolytica Po1g strain. We identified two media at the expense of which Y. lipolytica was able to accumulate ∼5 g L(-1) of biomass and 0.8 g L(-1) of fatty acids (0.16 g of fatty acids per g of dry weight). These optimized media contained 0.5 g L(-1) of urea or ammonium sulfate and 20 g L(-1) of glycerol, and were devoid of yeast extract. Moreover, Y. lipolytica was engineered by inserting the FatB2 gene, coding for the CpFatB2 thioesterase from Cuphea palustris, in order to modify the fatty acid composition towards the accumulation of medium-chain fatty acids. Contrary to the expected, the expression of the heterologous gene increased the production of oleic acid, and concomitantly decreased the level of saturated fatty acids.
[Mh] Termos MeSH primário: Engenharia Metabólica
Ácido Oleico/biossíntese
Proteínas de Plantas/biossíntese
Tioléster Hidrolases/biossíntese
[Mh] Termos MeSH secundário: Sulfato de Amônio/química
Biomassa
Reatores Biológicos
Carbono/metabolismo
Meios de Cultura
Cuphea/enzimologia
Glicerol/metabolismo
Ácido Oleico/metabolismo
Proteínas de Plantas/metabolismo
Tioléster Hidrolases/metabolismo
Yarrowia/enzimologia
Yarrowia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Plant Proteins); 2UMI9U37CP (Oleic Acid); 7440-44-0 (Carbon); EC 3.1.2.- (FatB2 protein, Cuphea palustris); EC 3.1.2.- (Thiolester Hydrolases); PDC6A3C0OX (Glycerol); SU46BAM238 (Ammonium Sulfate)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151101
[St] Status:MEDLINE
[do] DOI:10.1002/btpr.2189


  4 / 23 MEDLINE  
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[PMID]:26505880
[Au] Autor:Kim HJ; Silva JE; Iskandarov U; Andersson M; Cahoon RE; Mockaitis K; Cahoon EB
[Ad] Endereço:Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
[Ti] Título:Structurally divergent lysophosphatidic acid acyltransferases with high selectivity for saturated medium chain fatty acids from Cuphea seeds.
[So] Source:Plant J;84(5):1021-33, 2015 Dec.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lysophosphatidic acid acyltransferase (LPAT) catalyzes acylation of the sn-2 position on lysophosphatidic acid by an acyl CoA substrate to produce the phosphatidic acid precursor of polar glycerolipids and triacylglycerols (TAGs). In the case of TAGs, this reaction is typically catalyzed by an LPAT2 from microsomal LPAT class A that has high specificity for C18 fatty acids containing Δ9 unsaturation. Because of this specificity, the occurrence of saturated fatty acids in the TAG sn-2 position is infrequent in seed oils. To identify LPATs with variant substrate specificities, deep transcriptomic mining was performed on seeds of two Cuphea species producing TAGs that are highly enriched in saturated C8 and C10 fatty acids. From these analyses, cDNAs for seven previously unreported LPATs were identified, including cDNAs from Cuphea viscosissima (CvLPAT2) and Cuphea avigera var. pulcherrima (CpuLPAT2a) encoding microsomal, seed-specific class A LPAT2s and a cDNA from C. avigera var. pulcherrima (CpuLPATB) encoding a microsomal, seed-specific LPAT from the bacterial-type class B. The activities of these enzymes were characterized in Camelina sativa by seed-specific co-expression with cDNAs for various Cuphea FatB acyl-acyl carrier protein thioesterases (FatB) that produce a variety of saturated medium-chain fatty acids. CvLPAT2 and CpuLPAT2a expression resulted in accumulation of 10:0 fatty acids in the Camelina sativa TAG sn-2 position, indicating a 10:0 CoA specificity that has not been previously described for plant LPATs. CpuLPATB expression generated TAGs with 14:0 at the sn-2 position, but not 10:0. Identification of these LPATs provides tools for understanding the structural basis of LPAT substrate specificity and for generating altered oil functionalities.
[Mh] Termos MeSH primário: Aciltransferases/química
Cuphea/enzimologia
Ácidos Graxos/metabolismo
[Mh] Termos MeSH secundário: Aciltransferases/metabolismo
Cuphea/metabolismo
Mineração de Dados
Filogenia
Domínios Proteicos
Sementes/enzimologia
Sementes/metabolismo
Alinhamento de Sequência
Análise de Sequência de Proteína
Análise de Sequência de RNA
Especificidade por Substrato
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Fatty Acids); EC 2.3.- (Acyltransferases); EC 2.3.1.52 (2-acylglycerophosphate acyltransferase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151028
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13063


  5 / 23 MEDLINE  
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[PMID]:26319961
[Au] Autor:Lima Prando TB; Barboza LN; Gasparotto FM; Araújo Vde O; Slgnor Tirloni CA; de Souza LM; Lourenço EL; Gasparotto Junior A
[Ad] Endereço:Laboratório de Farmacologia e Toxicologia de Produtos Naturais, Universidade Paranaense, ,Curitiba, PR Brazil.
[Ti] Título:Ethnopharmacological investigation of the diuretic and hemodynamic properties of native species of the Brazilian biodiversity.
[So] Source:J Ethnopharmacol;174:369-78, 2015 Nov 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Although Echinodorus grandiflorus, Cuphea carthagenensis, and Phyllanthus tenellus infusions are used in Brazilian folk medicine due to their possible diuretic effect, none of these species was critically investigated as a diuretic drug. So, the aim of this study was to evaluate the possible acute diuretic activity of ethanol soluble fractions (ES) obtained from these species and assess the relationship between renal cortical blood flow and their antioxidant and hypotensive activity using normotensive Wistar rats. MATERIAL AND METHODS: The preparation obtained from E. grandiflorus (ES-EG), C. carthagenensis (ES-CC), and P. tenellus (ES-PT) infusions was orally administered in a single dose to rats. Urine excretion rate, pH, density, conductivity and Na(+), K(+), Cl(-) and HCO3(-) contents were measured in the urine of saline-loaded animals. Concentration of electrolytes, total protein, urea, creatinine, and angiotensin converting enzyme (ACE) activity were evaluated in collected serum. The involvement of the renal cortical blood flow and antioxidative activity in the hypotensive and diuretic effects was also determined. RESULTS: Water and Na(+), Cl(-) and Na(+) excretion rates were significantly increased by ES-EG, while urinary bicarbonate excretion was reduced. Moreover, ES obtained from E. grandiflorus was able to significantly increase renal blood flow and reduce mean arterial pressure and oxidative stress in "in vitro" and "in vivo" models. All other parameters evaluated were not affected by any treatment. CONCLUSION: The results presented here shown that the ES-EG obtained from E. grandiflorus leaves shown a significant diuretic and hypotensive activity and suggest that these effects could be related with an important renal and systemic vasodilator effect. In addition, it was shown for the first time that the pharmacological effects of ES obtained from P. tenellus and C. carthagenensis do not support its popular use as a diuretic agent.
[Mh] Termos MeSH primário: Biodiversidade
Diuréticos/farmacologia
Etnofarmacologia/métodos
Hemodinâmica/efeitos dos fármacos
Componentes Aéreos da Planta
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Alismataceae
Animais
Brasil/etnologia
Cuphea
Diuréticos/isolamento & purificação
Hemodinâmica/fisiologia
Masculino
Phyllanthus
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Diuretics); 0 (Plant Extracts)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151106
[Lr] Data última revisão:
151106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150831
[St] Status:MEDLINE


  6 / 23 MEDLINE  
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[PMID]:25969557
[Au] Autor:Kim HJ; Silva JE; Vu HS; Mockaitis K; Nam JW; Cahoon EB
[Ad] Endereço:Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
[Ti] Título:Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.
[So] Source:J Exp Bot;66(14):4251-65, 2015 Jul.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Seeds of members of the genus Cuphea accumulate medium-chain fatty acids (MCFAs; 8:0-14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from Cuphea, for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop Camelina sativa. Transcriptomes from Cuphea viscosissima and Cuphea pulcherrima developing seeds that accumulate >90% of C8 and C10 fatty acids revealed three FatB cDNAs (CpuFatB3, CvFatB1, and CpuFatB4) expressed predominantly in seeds and structurally divergent from typical FatB thioesterases that release 16:0 from acyl carrier protein (ACP). Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0. Co-expression of combinations of previously characterized Cuphea and California bay FatBs produced Camelina oils with mixtures of C8-C16 fatty acids, but amounts of each fatty acid were less than obtained by expression of individual FatB cDNAs. Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs. RNA interference (RNAi) suppression of Camelina ß-ketoacyl-ACP synthase II, however, reduced 12:0 in seeds expressing a 12:0-ACP-specific FatB. Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.
[Mh] Termos MeSH primário: Cuphea/metabolismo
Palmitoil-CoA Hidrolase/metabolismo
Sementes/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cuphea/embriologia
Cuphea/enzimologia
Ácidos Graxos/metabolismo
Dados de Sequência Molecular
Palmitoil-CoA Hidrolase/química
Folhas de Planta/metabolismo
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Fatty Acids); EC 3.1.2.2 (Palmitoyl-CoA Hydrolase)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erv225


  7 / 23 MEDLINE  
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[PMID]:25687972
[Au] Autor:Evans KO; Laszlo JA; Compton DL
[Ad] Endereço:Renewable Products Technology Research Unit, National Center of Agricultural Utilization Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA. Electronic address: Kervin.Evans@ars.usda.gov.
[Ti] Título:Hydroxytyrosol and tyrosol esters partitioning into, location within, and effect on DOPC liposome bilayer behavior.
[So] Source:Biochim Biophys Acta;1848(5):1175-82, 2015 May.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The phenols hydroxytyrosol and tyrosol made abundantly available through olive oil processing were enzymatically transesterified into effective lipophilic antioxidants with cuphea oil. The hydroxytyrosyl and tyrosyl esters made from cuphea oil were assessed for their ability to partition into, locate within and effect the bilayer behavior of 1,2-dioloeoylphosphatidylcholine liposomes and compared to their counterparts made from decanoic acid. Partitioning into liposomes was on the same scale for both hydroxytyrosyl derivatives and both tyrosyl derivatives. All were found to locate nearly at the same depth within the bilayer. Each was found to affect bilayer behavior in a distinct manner.
[Mh] Termos MeSH primário: Antioxidantes/química
Ésteres/química
Bicamadas Lipídicas
Álcool Feniletílico/análogos & derivados
Fosfatidilcolinas/química
[Mh] Termos MeSH secundário: Cuphea
Ácidos Decanoicos/química
Esterificação
Lipossomos
Modelos Químicos
Estrutura Molecular
Álcool Feniletílico/química
Óleos Vegetais/química
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Decanoic Acids); 0 (Esters); 0 (Lipid Bilayers); 0 (Liposomes); 0 (Phosphatidylcholines); 0 (Plant Oils); 10597-60-1 (3,4-dihydroxyphenylethanol); 1AK4MU3SNX (4-hydroxyphenylethanol); 4G9EDB6V73 (decanoic acid); EDS2L3ODLV (1,2-oleoylphosphatidylcholine); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150218
[St] Status:MEDLINE


  8 / 23 MEDLINE  
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[PMID]:24342782
[Au] Autor:Palacios-Espinosa JF; Arroyo-García O; García-Valencia G; Linares E; Bye R; Romero I
[Ad] Endereço:Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, C.P. 04510 México, D.F., México.
[Ti] Título:Evidence of the anti-Helicobacter pylori, gastroprotective and anti-inflammatory activities of Cuphea aequipetala infusion.
[So] Source:J Ethnopharmacol;151(2):990-8, 2014 Feb 03.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Cuphea aequipetala (Lythraceae) is a medicinal plant highly appreciated in Mexico to treat stomach ailments such as pain and burning sensation, stomach infections, ulcers, diarrhea, dysentery, and different types of tumors and bruises. In this work, the infusion of aerial parts of this plant (CAI) was investigated for its polypharmacological potential. MATERIALS AND METHODS: In vitro anti-Helicobacter pylori activity was assessed by broth dilution method. Pharmacological studies included acute toxicity in mice using Lorke´s model, anti-inflammatory activity by xylene and TPA induced ear edema assay, as well as gastroprotection with ethanol-induced gastric ulcer model. DPPH and ABTS assays were used to determine antioxidant capacity. Polyphenols and flavonoid contents were determined by Folin-Ciocalteu method and AlCl3 reaction, respectively. RESULTS: CAI showed good anti-Helicobacter pylori activity with a MIC of 125µg/mL. The infusion was not toxic according to Lorke's model with a LD50 greater than 5g/kg. CAI exhibited low anti-edematogenic action in the models assayed. Oral administration of 300mg/kg CAI significantly reduced gastric lesions by 87.9%. The effect was reversed only by indomethacin and N-ethylmaleimide demonstrating the role of endogenous prostaglandins and sulfhydryl compounds in gastroprotection. Total phenolic and flavonoid contents of CAI were 109.9mg GAE/g DW and 28.1mg QE/g DW, respectively, and the infusion exhibited a good antioxidant activity that is thought to play a role in its biological activity. The analysis of a preliminary fractionation of the infusion indicates that the complete extract conserves all its pharmacological activities in contrast to fractionated extracts. CONCLUSIONS: Cuphea aequipetala is a promising native herb in an integral therapy for the treatment of bacterial or non-bacterial gastric ulcer because it possesses some anti-inflammatory properties, as well as exhibits good gastroprotective and antibacterial effects. It represents an important source for the isolation of anti-Helicobacter pylori compounds. This work provides ethnopharmacological evidence that supports the traditional use of this species.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Antiulcerosos/uso terapêutico
Cuphea
Edema/tratamento farmacológico
Extratos Vegetais/uso terapêutico
Úlcera Gástrica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/farmacologia
Anti-Inflamatórios/toxicidade
Antiulcerosos/farmacologia
Antiulcerosos/toxicidade
Antioxidantes/farmacologia
Antioxidantes/uso terapêutico
Antioxidantes/toxicidade
Benzotiazóis/metabolismo
Edema/induzido quimicamente
Etanol
Flavonoides/análise
Helicobacter pylori/efeitos dos fármacos
Masculino
Camundongos
Componentes Aéreos da Planta
Extratos Vegetais/farmacologia
Extratos Vegetais/toxicidade
Polifenóis/análise
Úlcera Gástrica/induzido quimicamente
Ácidos Sulfônicos/metabolismo
Acetato de Tetradecanoilforbol
Testes de Toxicidade Aguda
Xilenos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Anti-Ulcer Agents); 0 (Antioxidants); 0 (Benzothiazoles); 0 (Flavonoids); 0 (Plant Extracts); 0 (Polyphenols); 0 (Sulfonic Acids); 0 (Xylenes); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); 3K9958V90M (Ethanol); NI40JAQ945 (Tetradecanoylphorbol Acetate)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140127
[Lr] Data última revisão:
140127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131218
[St] Status:MEDLINE


  9 / 23 MEDLINE  
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[PMID]:23454211
[Au] Autor:Tjellström H; Strawsine M; Silva J; Cahoon EB; Ohlrogge JB
[Ad] Endereço:Department of Plant Biology, Great Lakes Bioenergy Research Center Michigan State University, East Lansing, MI 48824, USA. henrik@msu.edu
[Ti] Título:Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis.
[So] Source:FEBS Lett;587(7):936-42, 2013 Apr 02.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Carbono-Enxofre Ligases/genética
Ácidos Graxos/metabolismo
Sementes/genética
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Carbono-Enxofre Ligases/metabolismo
Cuphea/enzimologia
Cuphea/genética
Ácidos Graxos/química
Mutação
Plantas Geneticamente Modificadas
Plastídeos/enzimologia
Plastídeos/genética
Sementes/metabolismo
Espectrometria de Massas por Ionização por Electrospray
Tioléster Hidrolases/genética
Tioléster Hidrolases/metabolismo
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Fatty Acids); 0 (Triglycerides); EC 3.1.2.- (Thiolester Hydrolases); EC 3.1.2.14 (oleoyl-(acyl-carrier-protein) hydrolase); EC 6.2.- (Carbon-Sulfur Ligases); EC 6.2.1.20 (long-chain-fatty-acid-(acyl-carrier-protein) ligase)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:130325
[Lr] Data última revisão:
130325
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130305
[St] Status:MEDLINE


  10 / 23 MEDLINE  
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[PMID]:22739415
[Au] Autor:Krepsky PB; Isidório RG; de Souza Filho JD; Côrtes SF; Braga FC
[Ad] Endereço:Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31.270-901 Belo Horizonte, Brazil.
[Ti] Título:Chemical composition and vasodilatation induced by Cuphea carthagenensis preparations.
[So] Source:Phytomedicine;19(11):953-7, 2012 Aug 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The aerial parts of Cuphea carthagenensis (Jacq.) J.F. Macbride (Lythraceae) are traditionally employed in Brazil to treat cardiovascular diseases. The aim of this study was to compare preparations of C. carthagenensis aerial parts (aqueous and ethanol extracts, together with derived fractions) with regard to their total phenolic contents and in vitro vasodilating activity. The main flavonoids found in the extracts were isolated and identified as quercetin derivatives. The extracts and fractions showed similar HPLC profiles with the presence of quercetin-5-O-ß-glucopyranoside, quercetin-3-O-α-arabinofuranoside and quercetin-3-sulfate in all of them, but marked differences in the contents of flavonoids, proanthocyanidins, tannis and total phenolics. Excepting the aqueous extract, all assayed preparations elicited vasodilatation on pre-contracted rat aortic rings in the range of pIC(50) 4.53±0.03 to 4.98±0.06. Polynomial regression analysis demonstrated the relationship between vasodilating activity and the contents of flavonoids (r(2)=0.5190), proanthocyanidins (r(2)=0.8016), tannins (r(2)=0.8041) and total phenolics (r(2)=0.6226), suggesting the participation of these compounds in the pharmacological effect and their potential use as chemical markers for the species.
[Mh] Termos MeSH primário: Cuphea/química
Flavonoides/farmacologia
Fenóis/farmacologia
Extratos Vegetais/farmacologia
Vasodilatação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Arabinose/análogos & derivados
Arabinose/química
Arabinose/farmacologia
Cromatografia Líquida de Alta Pressão
Sinergismo Farmacológico
Flavonoides/química
Flavonoides/isolamento & purificação
Glucosídeos/química
Glucosídeos/farmacologia
Masculino
Fenóis/isolamento & purificação
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Proantocianidinas/isolamento & purificação
Proantocianidinas/farmacologia
Quercetina/análogos & derivados
Quercetina/química
Quercetina/isolamento & purificação
Quercetina/farmacologia
Ratos
Ratos Wistar
Análise de Regressão
Taninos/isolamento & purificação
Taninos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Flavonoids); 0 (Glucosides); 0 (Phenols); 0 (Plant Extracts); 0 (Proanthocyanidins); 0 (Tannins); 0 (quercetin 3'-sulfate); 0 (quercetin-3-O-alpha-arabinofuranoside); 0 (quercetin-5-O-beta-glucopyranoside); 9IKM0I5T1E (Quercetin); B40ROO395Z (Arabinose)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:120822
[Lr] Data última revisão:
120822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120629
[St] Status:MEDLINE
[do] DOI:10.1016/j.phymed.2012.05.011



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